阅读说明：本技术 一种全植物蛋白罗氏沼虾育成配合饲料及其制备方法 (Compound feed bred from full-plant protein macrobrachium rosenbergii and preparation method of compound feed ) 是由 徐宏琴 刘波 王亚君 周和齐 万厚荣 于 2020-04-24 设计创作，主要内容包括：本发明公开了一种全植物蛋白罗氏沼虾育成配合饲料及其制备方法,属于水产动物配合饲料技术领域。该配合饲料的制备方法为：先将豆粕、花生粕、菜粕、普通面粉粉碎,然后与豆油、大豆磷脂油、大豆浓缩蛋白、谷朊粉、DDGS、膨润土、氯化钠、防霉剂、氯化胆碱、山道喹、辣木叶发酵产物、茶树油、鱿鱼膏、脱壳素、维生素、矿物元素、L-蛋氨酸、L-赖氨酸、诱食剂及磷酸二氢钙搅拌混合混匀；搅拌混匀后经过粉碎机粉碎为混合物料,最后利用颗粒饲料的常规方法制成颗粒饲料。本发明采用全植物蛋白原料不仅绿色环保,而且大大降低了饲料成本,并通过补充必需氨基酸及多种诱食剂,提高了饲料的营养价值,促进了罗氏沼虾生长。(The invention discloses a compound feed bred by using a full plant protein Macrobrachium rosenbergii and a preparation method thereof, belonging to the technical field of compound feeds for aquatic animals.)

1. The compound feed is characterized by comprising, by weight, 20.0-25.0 parts of soybean meal, 20.0-22.0 parts of soybean protein concentrate, 19.0-21.5 parts of common flour, 6.0-7.4 parts of peanut meal, 6.0-7.4 parts of rapeseed meal, 5.0-5.5 parts of gluten meal, 1.0-1.2 parts of soybean oil, 3.0-3.4 parts of soybean phospholipid oil, 3.5-4.4 parts of DDGS, 0.9-1.2 parts of bentonite, 1.8-2.2 parts of monocalcium phosphate, 0.15-0.17 part of sodium chloride, 0.18-0.22 part of choline chloride, 0.08-0.12 part of chomaquina, 0.08-0.12 part of a mildew preventive, 3.8-4.4 parts of squid extract, 0.08-0.22 part of ecdysone, 0.08-0.22 part of mineral, 0.84-0.18.18-0.18 part of mineral, 0.18-0.18 parts of lysine, 0.18-22 parts of a fermented oil, 0.9-3.2 parts of vitamin E, 0.3-3 parts of vitamin E, 0.3-0.2 parts of fermented tea plant protein concentrate and 0.3-3.3-3 parts of vitamin E.

2. The whole plant protein macrobrachium rosenbergii compound feed as claimed in claim 1, wherein the choline chloride content is 50%.

3. The whole plant protein macrobrachium rosenbergii compound feed as claimed in claim 1, wherein the phagostimulant is trimethylamine oxide.

4. A method for preparing the whole plant protein macrobrachium rosenbergii compound feed as claimed in claim 1, which comprises the following steps:

(1) primary mixing and crushing: crushing the bean pulp, the peanut pulp, the rapeseed meal and the common flour for the first time by a crusher at the crushing speed of 400-500r/min, passing through a 60-80 mesh sieve, and mixing with the soybean protein concentrate to obtain a mixed crude material;

(2) secondary material mixing: stirring the mixed crude material obtained in the step (1) with soybean oil, soybean phospholipid oil, tea tree oil, wheat gluten, DDGS, choline chloride, monocalcium phosphate, ecdysone, sodium chloride, squid liver paste, bentonite and the like, and uniformly mixing in a mixer to obtain a secondary mixed material; the feeding speed of the mixer is 18-20 r/min;

(3) and (3) secondary crushing: the secondary mixed material is crushed for the second time by a crusher and passes through a 60-80 mesh screen; the crushing speed of the crusher is 400-;

(4) mixing the materials for the third time, namely adding the mildew preventive, the behenic acid, the trimethylamine oxide phagostimulant, the vitamin additive, the mineral additive, the L-methionine, the L-lysine and the moringa oleifera leaf leavening into the mixture obtained by the secondary crushing in the step (3), uniformly mixing the mixture in a mixer to obtain a third mixed material, wherein the rotating speed of the mixer during feeding is 18-20r/min

(5) And (3) crushing for three times: pulverizing the third mixed material with a pulverizer for the third time, and sieving with a 60-80 mesh sieve; the crushing speed of the crusher is 400-

(6) And (3) post-treatment: preparing the mixture obtained by the three-time crushing in the step (5) into granulated feed by using an aquatic feed granulator; drying the granulated feed in a dryer at the temperature of 90-100 ℃; and cooling the dried feed in a cooler and packaging to obtain the compound feed bred by the whole plant protein Macrobrachium rosenbergii.

5. The whole plant protein macrobrachium rosenbergii compound feed as claimed in claim 4, wherein in step (6), the particle size of the pellet feed is 2.0mm-3.0 mm.

6. The method for preparing the complete plant protein macrobrachium rosenbergii compound feed according to claim 4, wherein in the step (4), the preparation process of the moringa oleifera leaf fermentation product comprises the following steps: crushing moringa leaves to 60-80 meshes to obtain moringa leaf powder; adding into water solution with pH of 4.0-6.0 at a weight ratio of Moringa oleifera leaf to water of 8-12: 1, placing in a fermentation tank, adding Bacillus amyloliquefaciens FFRC-S24 into the fermentation tank, setting the temperature of the fermentation tank at 30-40 deg.C, stirring at 4-8r/min, and culturing for 16-32 h; pouring the obtained fermentation liquor into a volumetric flask of a rotary evaporator, setting the temperature at 50-55 ℃ and the air pressure at 0.001-0.003MPa, repeatedly extracting for 2-4 times, concentrating the spin-out liquor obtained by 2-4 times in the rotary evaporator at the concentration temperature of 70-80 ℃ and the air pressure at 0.001-0.003MPa, and concentrating to obtain thick paste, namely obtaining the moringa oleifera leaf fermentation product.

7. The method for preparing the complete plant protein Macrobrachium rosenbergii breeding compound feed according to claim 6, wherein the aqueous solution with pH of 4.0-6.0 adopts acetic acid-sodium acetate as pH regulator.

8. The method for preparing the complete plant protein macrobrachium rosenbergii compound feed according to claim 6, wherein the bacillus amyloliquefaciens FFRC-S24 is preserved in the China center for type culture Collection in 2013, 5 and 9 months, with the preservation numbers as follows: CCTCC NO: and M2013191.

9. The method for preparing a complete plant protein Macrobrachium rosenbergii compound feed according to claim 6, wherein the concentration of Bacillus amyloliquefaciens FFRC-S24 is 100 hundred million CFU/g.

Technical Field

The invention belongs to the technical field of aquatic animal compound feeds, and particularly relates to a compound feed bred by using a full-plant protein macrobrachium rosenbergii and a preparation method thereof.

Background

Macrobrachium rosenbergii (Macrobrachium rosenbergii) belongs to the family brachialidae, also known as Macrobrachium malabaricum, Macrobrachium nipponensis, pseudobrachium amansii, Macrobrachium nipponensis, and the like, is native to the entire southern and southeast asia region, and northern oceania and western pacific islands, and lives in fresh or brackish water waters of these regions throughout the year. Compared with other domestic cultured shrimps, the macrobrachium rosenbergii has the advantages of large individual, wide eating habits, less diseases, easy survival, fast growth, good nutrition and the like, thereby having important economic value.

Proteins are essential nutrients for the structure and function of all organisms including shrimps, and are important in various nutrients, not only as constituents of various tissues and organs for reproduction and repair, but also as constituents of active substances such as enzymes, hormones, and antibodies. At the same time, protein is also the most important and expensive nutrient component in feed. The cost of the protein material in the compound feed often determines the feed price, and in the aquaculture process, the feed cost accounts for about 60% of the total operation cost of aquaculture, so that the increase of the aquaculture benefit benefits from the development of lower-cost aquaculture feed. The fish meal is always the high-quality preferred protein source of the aquatic feed due to the advantages of high protein content, complete amino acid types, high conversion utilization rate and the like. However, the rapid development of intensive aquaculture and the annual reduction of fishing resources for fish meal production lead to a shortage of fish meal supply and demand and a high price, thereby greatly increasing the cost of the compound feed. In addition, the fish meal contains higher non-digestible phosphorus content, and excessive use of the fish meal can increase phosphorus discharge of aquatic animals and cause water body pollution. Therefore, in view of the sustainable development of aquaculture, and in order to reduce feed cost, people are continuously looking for other novel protein sources with high quality and low price to replace animal proteins such as fish meal, and the plant protein source is the first choice of replacing proteins due to the low price and wide source. Aiming at the problems, the patent (201010608349.5) applied by Liyulan et al (2010) discloses a compound feed for breeding carp with full-plant protein and a preparation method thereof, wherein plant protein sources such as corn protein powder, pea protein powder, bean pulp and rapeseed meal are mainly used for replacing fish meal. Disclosed in patent application 201410285108.X of Zhonglan et al (2014), a megalobrama amblycephala puffed feed containing whole plant protein and a preparation method thereof. Liuwei et al (2019) applied for a patent (201910134141.5) of a compound feed with a whole plant protein source for procambarus clarkii and application thereof. However, no report is found about the compound feed of the macrobrachium rosenbergii total plant protein for replacing fish meal and the preparation method thereof.

Disclosure of Invention

Aiming at the problems in the prior art, the invention aims to provide a compound feed bred by using the whole plant protein Macrobrachium rosenbergii; the invention also provides a preparation method of the compound feed bred by the whole plant protein macrobrachium rosenbergii.

In order to solve the technical problems, the technical scheme adopted by the invention is as follows:

a compound feed for culturing macrobrachium rosenbergii is prepared from soybean cake 20.0-25.0 parts, soybean protein concentrate 20.0-22.0 parts, common flour 19.0-21.5 parts, peanut cake 6.0-7.4 parts, rapeseed cake 6.0-7.4 parts, wheat gluten 5.0-5.5 parts, soybean oil 1.0-1.2 parts, soybean phosphatide oil 3.0-3.4 parts, DDGS 3.5-4.4 parts, bentonite 0.9-1.2 parts, calcium dihydrogen phosphate 1.8-2.2 parts, sodium chloride 0.15-0.17 part, choline chloride 0.18-0.22 part, chololine 0.08-0.12 part, antimildew agent 0.08-0.12 part, squid liver paste 3.8-4.4 parts, capsaicine 0.08-0.12 part, molting element 0.18-0.84 part, mineral molting element 0.18-0.22 part, lysine 0.18-0.18.18.18-0.18.18.18.18.18-0.18.18.18 parts, lysine element 0.8528 parts, and methionine 0.07-0.3-2 parts.

Further, the choline chloride content was 50%.

Further, the phagostimulant is trimethylamine oxide.

A preparation method of a compound feed bred by using a whole plant protein Macrobrachium rosenbergii comprises the following steps:

(1) primary mixing and crushing: crushing the bean pulp, the peanut pulp, the rapeseed meal and the common flour for the first time by a crusher at the crushing speed of 400-500r/min, passing through a 60-80 mesh screen, and mixing with the soybean protein concentrate to obtain a mixed crude material;

(2) secondary material mixing: stirring the mixed crude material obtained in the step (1) with soybean oil, soybean lecithin oil, tea tree oil, wheat gluten, DDGS, choline chloride, monocalcium phosphate, ecdysone, sodium chloride, squid liver paste, bentonite and the like, and uniformly mixing in a mixer to obtain a secondary mixed material; the feeding speed of the mixer is 18-20 r/min;

(3) and (3) secondary crushing: the secondary mixed material is crushed for the second time by a crusher and passes through a 60-80 mesh screen; the crushing speed of the crusher is 400-;

(4) mixing the materials for the third time, namely adding the mildew preventive, the behenic acid, the trimethylamine oxide phagostimulant, the vitamin additive, the mineral additive, the L-methionine, the L-lysine and the moringa oleifera leaf leavening into the mixture obtained by the secondary crushing in the step (3), and uniformly mixing the mixture in a mixer to obtain a third mixed material, wherein the rotating speed of the mixer is 18-20r/min during feeding;

(5) and (3) crushing for three times: pulverizing the third mixed material with a pulverizer for the third time, and sieving with a 60-80 mesh sieve; the crushing speed of the crusher is 400-;

(6) and (3) post-treatment: preparing the mixture obtained after the three-time crushing in the step (5) into granulated feed by using an aquatic feed granulating machine; drying the granulated feed in a dryer at the temperature of 90-100 ℃; and cooling the dried feed in a cooler and packaging to obtain the compound feed bred by the whole plant protein Macrobrachium rosenbergii.

Further, in the step (6), the particle size of the pellet feed is 2.0mm-3.0 mm.

Further, in the step (4), the preparation process of the moringa oleifera leaf fermentation product comprises the following steps: crushing moringa leaves to 60-80 meshes to obtain moringa leaf powder; adding into water solution with pH of 4.0-6.0, the weight ratio of Moringa oleifera leaf to water is 8-12: 1, placing into a fermentation tank, adding Bacillus amyloliquefaciens FFRC-S24 into the fermentation tank, setting the temperature of the fermentation tank at 30-40 deg.C, stirring at 4-8r/min, and culturing for 16-32 h; pouring the obtained fermentation liquor into a volumetric flask of a rotary evaporator, setting the temperature at 50-55 ℃ and the air pressure at 0.001-0.003MPa, repeatedly extracting for 2-4 times, concentrating the spin-out liquor obtained by 2-4 times in the rotary evaporator at the concentration temperature of 70-80 ℃ and the air pressure at 0.001-0.003MPa, and concentrating to obtain thick paste, namely obtaining the moringa oleifera leaf fermentation product.

Furthermore, the aqueous solution with the pH of 4.0-6.0 adopts acetic acid-sodium acetate as a pH regulator.

Further, bacillus amyloliquefaciens FFRC-S24 is deposited in the chinese type culture collection center at 2013, 5, 9 months, with the deposit numbers: CCTCC NO: and M2013191.

Further, the concentration of Bacillus amyloliquefaciens FFRC-S24 is 100 hundred million CFU/g.

Has the advantages that: compared with the prior art, the invention has the advantages that:

1) the feed adopts pure plant protein raw materials, is low in price and environment-friendly, and is added with the essential amino acids of L-lysine and L-methionine, so that the content deficiency of the lysine and the methionine in the plant protein is improved, and the quality of the protein and the amino acid of the feed is guaranteed;

2) the feed is added with the moringa oleifera leaf fermentation product and the tea tree oil immunopotentiator, so that the anti-stress and disease-resistant capability of shrimps can be enhanced, and the stress hazard can be reduced;

3) the selected raw materials source of this application fodder is sufficient, has adopted cubic mixing and cubic kibbling technology, guarantees feed ingredient's crushing granularity and mixing uniformity, increases the stability of shrimp feed in aqueous, improves the fodder rate of utilization.

Detailed Description

The present invention will be further described with reference to the following examples, but the present invention is not limited to the following examples.

L-methionine and L-lysine in the following examples were provided by Zhengzhou Furun bioengineering, Inc., choline chloride content of 50% was provided by Hebei Anhua feed additives factory, ecdysone was provided by Shandong Huawei Biotech Co., Ltd, fungicide was provided by Ningbo Wanglong Biotech Co., Ltd, trimethylamine oxide was provided by Ningbo Huayuan Biotech Co., Ltd, and vitamins and mineral elements were provided by Wuxi Huanwei animal health care Limited.