阅读说明：本技术 一种基于甲基丙烯酸化水凝胶/纳米粘土/脱细胞基质的复合生物墨水及其制备方法和应用 (Composite biological ink based on methacrylated hydrogel/nanoclay/acellular matrix and preparation method and application thereof ) 是由 张秀莉 庄甜甜 罗勇 于 2020-04-03 设计创作，主要内容包括：本发明公开了一种基于甲基丙烯酸化水凝胶/纳米粘土/脱细胞基质的复合生物墨水,所述复合生物墨水的组分包括脱细胞基质溶液、光交联甲基丙烯酸化水凝胶以及纳米黏土。本发明还公开了所述的基于甲基丙烯酸化水凝胶/纳米粘土/脱细胞基质的复合生物墨水的制备方法及应用。本发明的基于甲基丙烯酸化水凝胶/纳米粘土/脱细胞基质的复合生物墨水,表征效果好,可打印性强,生物相容性好。(The invention discloses a composite biological ink based on methacrylated hydrogel/nanoclay/acellular matrix, which comprises the components of an acellular matrix solution, photo-crosslinked methacrylated hydrogel and nanoclay. The invention also discloses a preparation method and application of the methacrylic acid hydrogel/nano clay/acellular matrix-based composite biological ink. The composite biological ink based on the methacrylated hydrogel/nanoclay/acellular matrix has the advantages of good representation effect, strong printability and good biocompatibility.)

1. The composite biological ink based on the methacrylated hydrogel/nanoclay/acellular matrix is characterized by comprising the components of photo-crosslinked methacrylated hydrogel, an acellular matrix solution and nanoclay.

2. The methacrylated hydrogel/nanoclay/acellular matrix-based composite bio-ink of claim 1, wherein said photocrosslinked methacrylated hydrogel is present at a concentration of 1% to 50%.

3. The methacrylated hydrogel/nanoclay/acellular matrix-based composite bio-ink of claim 1, wherein said acellular matrix solution is an acellular matrix pepsin solution of the liver, kidneys, nerves, brain, intestine, gallbladder, pancreas, heart, lung, stomach, spleen, blood vessels, muscles, lymph, ears, nose, eyes of an animal or human.

4. The methacrylated hydrogel/nanoclay/acellular matrix-based composite bio-ink of claim 1, wherein the nanoclay is any one or more of the following classes: bentonite, montmorillonite, kaolinite, hectorite and halloysite.

5. The methacrylated hydrogel/nanoclay/acellular matrix-based composite bio-ink of claim 1, wherein the concentration of the acellular matrix solution is 1-20 mg/ml.

6. The methacrylated hydrogel/nanoclay/acellular matrix-based composite bio-ink of claim 1, wherein the volume ratio of the photo-crosslinked methacrylated hydrogel to the acellular matrix solution is 5:1 to 1: 5.

7. The methacrylated hydrogel/nanoclay/acellular matrix-based composite bio-ink of claim 1, wherein the nanoclay is added in an amount of 1% to 10% of the total mass of the acellular matrix solution and the photo-crosslinked methacrylated hydrogel.

8. The methacrylated hydrogel/nanoclay/acellular matrix-based composite bio-ink of claim 1, wherein the cells for printing the composite bio-ink include cells of the liver, kidney, nerves, brain, intestine, pancreas, heart, lung, stomach, spleen, gallbladder, blood vessels, muscle, lymph, ear, nose, eye.

9. The method for preparing a methacrylated hydrogel/nanoclay/acellular matrix-based composite bio-ink according to any one of claims 1 to 8, comprising:

s1, freeze-drying and grinding the acellular matrix, and adding a pepsin solution for dissolving to prepare an acellular matrix solution;

and S2, mixing the obtained acellular matrix solution with the photo-crosslinking methacrylic acid hydrogel, adding nano clay, and uniformly mixing to obtain the composite biological ink.

10. The method for preparing a composite bio-ink based on methacrylated hydrogel/nanoclay/acellular matrix according to claim 9, wherein in step S1, the acellular matrix is obtained by performing acellular perfusion by a thawed tissue connecting peristaltic pump, and the acellular perfusion comprises in sequence:

(1)0.01 mol/L PBS perfusion for 1 h;

(2) 1% Triton X-100 perfuse for 1 h;

(3) after washing with PBS for 15min, 0.1% SDS was perfused for 30 min;

(4) after washing with PBS for 15min, pouring with DNase of 80U/ml and RNase of 5U/ml for 30 min;

(5) 2% penicillin-streptomycin, 2.5. mu.g/ml amphotericin B in PBS for 30 min.

11. The method of claim 9, wherein the pepsin solution is obtained by dissolving pepsin in hydrochloric acid, and the concentration of the pepsin solution is 0.5-10mg/m L in the step S1.

12. Use of the composite bio-ink according to any one of claims 1 to 8 for the construction of tissue engineering scaffolds.