阅读说明：本技术 一种中草药抑菌剂及其制备方法 (Chinese herbal medicine bacteriostatic agent and preparation method thereof ) 是由 杨祥鹏 许长剑 于 2021-06-22 设计创作，主要内容包括：本发明提供了一种中草药抑菌剂及其制备方法,包括以下重量份组分,中草药提取液20-30份、增稠剂0.8-1.2份、甘油0.5-1.5份、氯化钠1-3份、香精0.03-0.08份、纯水65-80份；所述中草药提取液包括以下重量份组分,丁香1-2份,大黄0.5-0.8份,肉桂0.2-0.6份,黄连0.8-1.2份,紫草0.1-0.3份,麻黄0.2-0.5份,细叶杜香0.1-0.3份,乙醇35-45份,纯水270-290份。本发明制备得到的中药草抑菌剂去污能力强,抑菌性能好,温和无刺激。(The invention provides a Chinese herbal medicine bacteriostatic agent and a preparation method thereof, wherein the bacteriostatic agent comprises the following components, by weight, 20-30 parts of a Chinese herbal medicine extracting solution, 0.8-1.2 parts of a thickening agent, 0.5-1.5 parts of glycerol, 1-3 parts of sodium chloride, 0.03-0.08 part of essence and 65-80 parts of pure water; the Chinese herbal medicine extracting solution comprises, by weight, 1-2 parts of clove, 0.5-0.8 part of rhubarb, 0.2-0.6 part of cinnamon, 0.8-1.2 parts of coptis chinensis, 0.1-0.3 part of lithospermum, 0.2-0.5 part of ephedra, 0.1-0.3 part of ledum palustre, 35-45 parts of ethanol and 290 parts of pure water. The traditional Chinese medicine grass bacteriostatic agent prepared by the invention has the advantages of strong decontamination capability, good bacteriostatic performance, mildness and no stimulation.)

1. A Chinese herbal medicine bacteriostatic agent is characterized by comprising the following components, by weight, 20-30 parts of a Chinese herbal medicine extracting solution, 0.8-1.2 parts of a thickening agent, 0.5-1.5 parts of glycerol, 1-3 parts of sodium chloride, 0.03-0.08 part of essence and 65-80 parts of pure water;

the Chinese herbal medicine extracting solution comprises, by weight, 1-2 parts of clove, 0.5-0.8 part of rhubarb, 0.2-0.6 part of cinnamon, 0.8-1.2 parts of coptis chinensis, 0.1-0.3 part of lithospermum, 0.2-0.5 part of ephedra, 0.1-0.3 part of ledum palustre, 35-45 parts of ethanol and 290 parts of pure water.

2. The Chinese herbal medicine bacteriostatic agent according to claim 1, which is characterized by comprising the following components, by weight, 25 parts of a Chinese herbal medicine extracting solution, 1 part of a thickening agent, 1 part of glycerol, 2 parts of sodium chloride, 0.05 part of essence and 72 parts of pure water.

3. The Chinese herbal medicine bacteriostatic agent according to claim 1, wherein the Chinese herbal medicine extract comprises, by weight, 1.5 parts of clove, 0.6 parts of rhubarb, 0.4 parts of cinnamon, 1 part of coptis chinensis, 0.2 parts of lithospermum, 0.3 parts of ephedra, 0.2 parts of ledum palustre, 41 parts of ethanol and 280 parts of pure water.

4. The herbal bacteriostatic agent as claimed in claim 1, wherein the thickener is one or more selected from RH-300, RH-960 and SDS.

5. The method for preparing the herbal bacteriostatic agent of claim 1, which comprises the following steps:

(1) pretreatment of Chinese herbal medicines: removing impurities from various Chinese herbal medicines, cutting into sections, then crushing and sieving with a 60-mesh sieve, and taking undersize materials for later use;

(2) preparing single-medicine extracting solutions of clove, rhubarb, cinnamon, coptis, lithospermum, ephedra and ledum palustre respectively;

(3) compounding the Chinese herbal medicine extracting solution: mixing the single Chinese herbal medicine extracting solutions in equal proportion, and uniformly stirring to obtain a Chinese herbal medicine extracting solution;

(4) preparing a hand sanitizer: adding the Chinese herbal medicine extracting solution obtained in the step (3) and pure water into a container according to a proportion, stirring and mixing uniformly, then adding a thickening agent and glycerol, and stirring until the mixture is uniformly dispersed; and dissolving sodium chloride by using pure water, adding the solution into a container while stirring, adjusting the pH of the solution to 4.5-6.5 by using citric acid, finally adding essence, and uniformly stirring and mixing to obtain the Chinese herbal medicine bacteriostatic hand sanitizer.

6. The method for preparing herbal bacteriostat according to claim 5, wherein the preparation method of each herbal extract is as follows:

firstly, clove: adding 65-80% ethanol solution into crushed clove according to the ratio of material to liquid of 1:10-1:20, performing ultrasonic extraction for 40-50min at the temperature of 35-50 ℃, filtering after extraction, taking supernatant, performing rotary evaporation to dryness, adding 20-30 parts of ethanol for redissolution, and diluting with 180 parts of pure water 160-180 parts to prepare a clove extracting solution;

② rhubarb: adding 70-90% ethanol solution into the crushed rhubarb according to the material-liquid ratio of 1:40-1:50, performing ultrasonic extraction for 20-30min at the temperature of 85-95 ℃, filtering after extraction, taking supernatant, performing rotary evaporation to dryness, adding 20-30 parts of ethanol for redissolution, and diluting by using 160-180 parts of pure water to prepare rhubarb extracting solution;

③ cinnamon: adding acetone solution into crushed cinnamon according to the material-liquid ratio of 1:35-1:45, performing ultrasonic extraction for 15-30min at the temperature of 30-50 ℃, filtering after extraction, taking supernate, performing rotary evaporation to dryness, adding 20-30 parts of ethanol for redissolution, and diluting with 180 parts of pure water with 160-180 parts of acetone to prepare cinnamon extracting solution;

fourthly, coptis: taking the crushed coptis, adding 60-70% ethanol solution according to the material-liquid ratio of 1:20-1:25, carrying out ultrasonic extraction for 70-90min at the temperature of 70-80 ℃, filtering after extraction, taking supernatant, carrying out rotary evaporation to dryness, adding 20-30 parts of ethanol for redissolution, and diluting with 180 parts of pure water 160-180 parts to prepare rhubarb extracting solution;

gromwell: taking the crushed lithospermum, adding 85-95% ethanol solution according to the material-liquid ratio of 1:10-1:15, performing ultrasonic extraction for 7-12min at the temperature of 75-85 ℃, filtering after the extraction is finished, taking the supernatant, performing rotary evaporation to dryness, adding 20-30 parts of ethanol for redissolution, and diluting by using 160-180 parts of pure water to prepare lithospermum extracting solution;

sixthly, the Chinese ephedra: adding 0.55-0.70mol/L hydrochloric acid solution into the crushed ephedra according to the material-liquid ratio of 1:12-1:20, stirring and leaching for 1.5-2.5h at the temperature of 70-80 ℃, repeatedly extracting for 2-4 times, filtering after extraction is finished, taking the supernatant, rotating and evaporating to dryness, adding 20-30 parts of ethanol for redissolution, and diluting by using 180 parts of pure water to prepare an ephedra extract;

seventhly, fine leaf ledum palustre: adding the crushed ledum fine-leaf into an absolute ethanol solution according to the material-liquid ratio of 1:20-1:25, stirring and leaching for 3-4h at the temperature of 65-75 ℃, filtering after extraction, taking supernate, performing rotary evaporation to dryness, adding 20-30 parts of ethanol for redissolution, and diluting with 180 parts of pure water 160-180 parts to prepare the ledum fine-leaf extracting solution.

7. The method of claim 5, wherein in the process for preparing the herbal extract, the ratio of the herbal extract to the liquid is respectively: 1:15 of clove, 1:45 of rhubarb, 1:40 of cinnamon, 1:22 of coptis chinensis, 1:13 of lithospermum, 1:15 of ephedra and 1:23 of ledum palustre.

8. The method of claim 5, wherein in the process for preparing the herbal extract, the extraction solutions are: flos Caryophylli is 75% ethanol solution, radix et rhizoma Rhei is 80% ethanol solution, cortex Cinnamomi is acetone solution, Coptidis rhizoma is 65% ethanol solution, radix Arnebiae is 90% ethanol solution, herba Ephedrae is 0.65mol/L hydrochloric acid solution, and Ledum Palustre L is anhydrous ethanol.

9. The method for preparing Chinese herbal medicine bacteriostatic agent according to claim 5, wherein in the preparation process of the Chinese herbal medicine extracting solution, the extracting temperatures are respectively as follows: flos Caryophylli of 45 deg.C, radix et rhizoma Rhei of 90 deg.C, cortex Cinnamomi of 40 deg.C, Coptidis rhizoma of 75 deg.C, radix Arnebiae of 80 deg.C, herba Ephedrae of 75 deg.C, and Ledum Palustre of 70 deg.C.

10. The method for preparing Chinese herbal medicine bacteriostatic agent according to claim 5, wherein in the preparation process of the Chinese herbal medicine extracting solution, the extracting time is respectively as follows: flos Caryophylli for 45min, radix et rhizoma Rhei for 25min, cortex Cinnamomi for 23min, Coptidis rhizoma for 80min, radix Arnebiae for 9min, herba Ephedrae for 2h, and Ledum Palustre for 3.5 h.

Technical Field

The invention belongs to the technical field of bacteriostasis, and particularly relates to a Chinese herbal medicine bacteriostat and a preparation method thereof.

Background

Pathogenic microorganisms existing in the environment have great threat to human health, and especially if the human body is contacted with objects containing the pathogenic microorganisms and cannot be disinfected in time, the pathogenic microorganisms can invade the human body again to cause health problems. Among them, the hands and arms of a human body are one of important body parts that are easily exposed to the external environment. In order to better achieve the purpose of disinfecting and inhibiting bacteria on hands and arms, the hand sanitizer is more and more widely applied.

At present, most of hand sanitizer products are added with chemical synthetic bacteriostatic agents and preservatives in the production process, although the purpose of disinfecting and bacteriostasis of hands and arms can be achieved, the hand sanitizer containing the chemical synthetic bacteriostatic agents and the preservatives has strong irritation to human bodies after long-term use, and even a part of the chemical synthetic bacteriostatic agents have the risk of carcinogenesis. Therefore, the development of a hand sanitizer product which does not contain chemically synthesized bacteriostat and preservative, has good bacteriostasis performance and is mild and non-irritating to skin is urgently needed

Disclosure of Invention

Aiming at the defects in the prior art, the invention provides the bacteriostatic agent prepared by extracting natural Chinese herbal medicines, and the hand sanitizer product prepared by using the bacteriostatic agent has the advantages of strong bacteriostatic ability, mild and non-irritating skin and strong bacteriostatic ability.

In order to achieve the purpose, the invention adopts the following technical scheme:

a Chinese herbal medicine bacteriostatic agent comprises the following components, by weight, 20-30 parts of a Chinese herbal medicine extracting solution, 0.8-1.2 parts of a thickening agent, 0.5-1.5 parts of glycerol, 1-3 parts of sodium chloride, 0.03-0.08 part of essence and 65-80 parts of pure water;

the Chinese herbal medicine extracting solution comprises, by weight, 1-2 parts of clove, 0.5-0.8 part of rhubarb, 0.2-0.6 part of cinnamon, 0.8-1.2 parts of coptis chinensis, 0.1-0.3 part of lithospermum, 0.2-0.5 part of ephedra, 0.1-0.3 part of ledum palustre, 35-45 parts of ethanol and 290 parts of pure water.

Preferably, the traditional Chinese medicine composition comprises, by weight, 25 parts of a Chinese herbal medicine extracting solution, 1 part of a thickening agent, 1 part of glycerol, 2 parts of sodium chloride, 0.05 part of essence and 72 parts of pure water.

Preferably, the Chinese herbal medicine extract comprises, by weight, 1.5 parts of clove, 0.6 part of rhubarb, 0.4 part of cinnamon, 1 part of coptis chinensis, 0.2 part of lithospermum, 0.3 part of ephedra, 0.2 part of ledum palustre, 41 parts of ethanol and 280 parts of pure water.

Preferably, the thickening agent is one or a mixture of RH-300, RH-960 and SDS.

A preparation method of a Chinese herbal medicine bacteriostatic agent comprises the following steps:

(1) pretreatment of Chinese herbal medicines: removing impurities from various Chinese herbal medicines, cutting into 1-2cm segments, pulverizing, sieving with 60 mesh sieve, and collecting undersize;

(2) preparing a single Chinese herbal medicine extracting solution: preparing various Chinese herbal medicines into extracting solutions according to the following extracting processes respectively;

firstly, clove: adding 65-80% ethanol solution into crushed clove according to the ratio of material to liquid of 1:10-1:20, performing ultrasonic extraction for 40-50min at the temperature of 35-50 ℃, filtering after extraction, taking supernatant, performing rotary evaporation to dryness, adding 20-30 parts of ethanol for redissolution, and diluting with 180 parts of pure water 160-180 parts to prepare a clove extracting solution;

② rhubarb: adding 70-90% ethanol solution into the crushed rhubarb according to the material-liquid ratio of 1:40-1:50, performing ultrasonic extraction for 20-30min at the temperature of 85-95 ℃, filtering after extraction, taking supernatant, performing rotary evaporation to dryness, adding 20-30 parts of ethanol for redissolution, and diluting by using 160-180 parts of pure water to prepare rhubarb extracting solution;

③ cinnamon: adding acetone solution into crushed cinnamon according to the material-liquid ratio of 1:35-1:45, performing ultrasonic extraction for 15-30min at the temperature of 30-50 ℃, filtering after extraction, taking supernate, performing rotary evaporation to dryness, adding 20-30 parts of ethanol for redissolution, and diluting with 180 parts of pure water with 160-180 parts of acetone to prepare cinnamon extracting solution;

fourthly, coptis: taking the crushed coptis, adding 60-70% ethanol solution according to the material-liquid ratio of 1:20-1:25, carrying out ultrasonic extraction for 70-90min at the temperature of 70-80 ℃, filtering after extraction, taking supernatant, carrying out rotary evaporation to dryness, adding 20-30 parts of ethanol for redissolution, and diluting with 180 parts of pure water 160-180 parts to prepare rhubarb extracting solution;

gromwell: taking the crushed lithospermum, adding 85-95% ethanol solution according to the material-liquid ratio of 1:10-1:15, performing ultrasonic extraction for 7-12min at the temperature of 75-85 ℃, filtering after the extraction is finished, taking the supernatant, performing rotary evaporation to dryness, adding 20-30 parts of ethanol for redissolution, and diluting by using 160-180 parts of pure water to prepare lithospermum extracting solution;

sixthly, the Chinese ephedra: adding 0.55-0.70mol/L hydrochloric acid solution into the crushed ephedra according to the material-liquid ratio of 1:12-1:20, stirring and leaching for 1.5-2.5h at the temperature of 70-80 ℃, repeatedly extracting for 2-4 times, filtering after extraction is finished, taking the supernatant, rotating and evaporating to dryness, adding 20-30 parts of ethanol for redissolution, and diluting by using 180 parts of pure water to prepare an ephedra extract;

seventhly, fine leaf ledum palustre: adding the crushed ledum fine-leaf into an absolute ethanol solution according to the material-liquid ratio of 1:20-1:25, stirring and leaching for 3-4h at the temperature of 65-75 ℃, filtering after extraction, taking supernate, performing rotary evaporation to dryness, adding 20-30 parts of ethanol for redissolution, and diluting with 180 parts of pure water 160-180 parts to prepare the ledum fine-leaf extracting solution.

(3) Compounding the Chinese herbal medicine extracting solution: mixing the single Chinese herbal medicine extracting solutions in equal proportion, uniformly stirring, and preparing a mixed solution to obtain a Chinese herbal medicine extracting solution;

(4) preparing a hand sanitizer: adding the Chinese herbal medicine extracting solution obtained in the step (3) and pure water into a container according to a proportion, stirring and mixing uniformly, then adding a thickening agent and glycerol, and stirring until the mixture is uniformly dispersed; and dissolving sodium chloride with a small amount of pure water, adding into a container while stirring, adjusting the pH of the solution to 4.5-6.5 with citric acid, adding essence, and stirring and mixing uniformly to obtain the Chinese herbal medicine bacteriostatic hand sanitizer.

Preferably, in the preparation process of the Chinese herbal medicine extracting solution, the material-liquid ratio is respectively as follows: 1:15 of clove, 1:45 of rhubarb, 1:40 of cinnamon, 1:22 of coptis chinensis, 1:13 of lithospermum, 1:15 of ephedra and 1:23 of ledum palustre.

Preferably, in the preparation process of the Chinese herbal medicine extracting solution, the extracting solutions are respectively: flos Caryophylli is 75% ethanol solution, radix et rhizoma Rhei is 80% ethanol solution, cortex Cinnamomi is acetone solution, Coptidis rhizoma is 65% ethanol solution, radix Arnebiae is 90% ethanol solution, herba Ephedrae is 0.65mol/L hydrochloric acid solution, and Ledum Palustre L is anhydrous ethanol.

Preferably, in the preparation process of the Chinese herbal medicine extracting solution, the extracting temperatures are respectively as follows: flos Caryophylli of 45 deg.C, radix et rhizoma Rhei of 90 deg.C, cortex Cinnamomi of 40 deg.C, Coptidis rhizoma of 75 deg.C, radix Arnebiae of 80 deg.C, herba Ephedrae of 75 deg.C, and Ledum Palustre of 70 deg.C.

Preferably, in the preparation process of the Chinese herbal medicine extracting solution, the extracting time is respectively as follows: flos Caryophylli for 45min, radix et rhizoma Rhei for 25min, cortex Cinnamomi for 23min, Coptidis rhizoma for 80min, radix Arnebiae for 9min, herba Ephedrae for 2h, and Ledum Palustre for 3.5 h.

Compared with the prior art, the invention has the beneficial effects that:

(1) the bacteriostatic agent is prepared by extracting natural Chinese herbal medicines, does not contain chemical synthetic bacteriostatic agents and preservatives, and is high in safety and mild and non-irritating to skin;

(2) according to the invention, multiple Chinese herbal medicines are compounded, and different extraction processes are adopted according to the characteristics of different Chinese herbal medicines, so that the antibacterial range is wide, the antibacterial capacity is strong, and the disinfection and antibacterial effects are good;

(3) the invention adopts natural Chinese herbal medicine extracts, thickening agents, glycerin and other substances to be compounded, and has excellent cleaning and bacteriostatic effects through the synergistic effect of various materials.

Detailed Description

The present invention is further described in detail below with reference to specific examples so that those skilled in the art can more clearly understand the present invention.

Example 1

A Chinese herbal medicine bacteriostatic agent is prepared by the following steps:

(1) pretreatment of Chinese herbal medicines: removing impurities from flos Caryophylli, radix et rhizoma Rhei, cortex Cinnamomi, Coptidis rhizoma, radix Arnebiae, herba Ephedrae, and Ledum Palustre L, cutting into 1-2cm segments, pulverizing, sieving with 60 mesh sieve, and collecting the undersize product;

(2) preparing a Chinese herbal medicine extracting solution: preparing various Chinese herbal medicines into extracting solutions according to the following extracting processes respectively;

firstly, clove: taking 1 part of crushed clove, adding 65% ethanol solution according to the material-liquid ratio of-1: 20, carrying out ultrasonic extraction for 50min at the temperature of 35 ℃, filtering after extraction, taking supernate, carrying out rotary evaporation to dryness, then adding 22 parts of ethanol for redissolving, and diluting with 176 parts of pure water to prepare a clove extracting solution;

② rhubarb: taking 0.8 part of the crushed rhubarb, adding 90% ethanol solution according to the material-to-liquid ratio of 1:45, carrying out ultrasonic extraction for 30min at the temperature of 95 ℃, filtering after the extraction is finished, taking supernate, carrying out rotary evaporation to dryness, then adding 22 parts of ethanol for redissolving, and diluting with 176 parts of pure water to prepare rhubarb extract;

③ cinnamon: taking 0.2 part of crushed cinnamon, adding an acetone solution according to the material-to-liquid ratio of 1:45, carrying out ultrasonic extraction for 18min at the temperature of 40 ℃, filtering after extraction is finished, taking supernate, carrying out rotary evaporation to dryness, then adding 22 parts of ethanol for redissolving, and diluting with 176 parts of pure water to prepare a cinnamon extracting solution;

fourthly, coptis: taking 1 part of the crushed coptis chinensis, adding 70% ethanol solution according to the ratio of material to liquid of 1:20, carrying out ultrasonic extraction for 70min at the temperature of 80 ℃, filtering after extraction, taking supernate, carrying out rotary evaporation to dryness, then adding 22 parts of ethanol for redissolution, and diluting with 176 parts of pure water to prepare coptis chinensis extract;

gromwell: taking 0.3 part of crushed lithospermum, adding 95% ethanol solution according to the material-liquid ratio of 1:10, performing ultrasonic extraction for 8min at the temperature of 80 ℃, filtering after extraction, taking supernate, performing rotary evaporation to dryness, then adding 22 parts of ethanol for redissolving, and diluting with 176 parts of pure water to prepare lithospermum extracting solution;

sixthly, the Chinese ephedra: taking 0.5 part of the crushed ephedra, adding 0.55mol/L hydrochloric acid solution according to the ratio of material to liquid of 1:18, stirring and leaching for 2 hours at the temperature of 77 ℃, repeatedly extracting for 2 times, filtering after the extraction is finished, taking supernate, rotating and evaporating to dryness, then adding 22 parts of ethanol for redissolution, and diluting with 176 parts of pure water to prepare an ephedra extract;

seventhly, fine leaf ledum palustre: taking 0.3 part of the crushed ledum minutissima, adding an absolute ethanol solution according to the material-liquid ratio of 1:25, stirring and extracting for 3 hours at the temperature of 70 ℃, filtering after extraction, taking supernate, carrying out rotary evaporation to dryness, then adding 22 parts of ethanol for redissolving, and diluting with 176 parts of pure water to prepare the ledum minutissima extract.

(3) Compounding the Chinese herbal medicine extracting solution: mixing the single Chinese herbal medicine extracting solutions in equal proportion, uniformly stirring, and preparing a mixed solution to obtain a Chinese herbal medicine extracting solution;

(4) preparing a hand sanitizer: adding 20 parts of the Chinese herbal medicine extract obtained in the step (3) and 80 parts of pure water into a container, stirring and mixing uniformly, then adding 1.2 parts of RH-300 and 0.5 part of glycerol, and stirring until the mixture is uniformly dispersed; and dissolving 1 part of sodium chloride by using a small amount of pure water, adding the solution into a container while stirring, adjusting the pH of the solution to 4.5-6.5 by using citric acid, finally adding 0.08 part of essence, and uniformly stirring and mixing to obtain the Chinese herbal medicine bacteriostatic hand sanitizer.

Example 2

A Chinese herbal medicine bacteriostatic agent is prepared by the following steps:

(1) pretreatment of Chinese herbal medicines: removing impurities from flos Caryophylli, radix et rhizoma Rhei, cortex Cinnamomi, Coptidis rhizoma, radix Arnebiae, herba Ephedrae, and Ledum Palustre L, cutting into 1-2cm segments, pulverizing, sieving with 60 mesh sieve, and collecting the undersize product;

(2) preparing a Chinese herbal medicine extracting solution: preparing various Chinese herbal medicines into extracting solutions according to the following extracting processes respectively;

firstly, clove: taking 2 parts of crushed clove, adding 80% ethanol solution according to the ratio of material to liquid of 1:20, carrying out ultrasonic extraction for 40min at the temperature of 35 ℃, filtering after extraction is finished, taking supernate, carrying out rotary evaporation to dryness, then adding 30 parts of ethanol for redissolving, and diluting with 165 parts of pure water to prepare a clove extracting solution;

② rhubarb: taking 0.5 part of crushed rhubarb, adding 80% ethanol solution according to the ratio of material to liquid of 1:50, carrying out ultrasonic extraction for 20min at the temperature of 85 ℃, filtering after extraction, taking supernate, carrying out rotary evaporation to dryness, then adding 30 parts of ethanol for redissolution, and diluting with 165 parts of pure water to prepare rhubarb extract;

③ cinnamon: taking 0.2 part of crushed cinnamon, adding an acetone solution according to a material-to-liquid ratio of 1:40, carrying out ultrasonic extraction for 25min at the temperature of 30 ℃, filtering after extraction is finished, taking supernate, carrying out rotary evaporation to dryness, then adding 30 parts of ethanol for redissolving, and diluting with 165 parts of pure water to prepare a cinnamon extracting solution;

fourthly, coptis: taking 1.2 parts of crushed coptis chinensis, adding 70% ethanol solution according to the ratio of material to liquid of 1:25, carrying out ultrasonic extraction for 90min at the temperature of 72 ℃, filtering after extraction, taking supernate, carrying out rotary evaporation to dryness, then adding 30 parts of ethanol for redissolution, and diluting with 165 parts of pure water to prepare rhubarb extract;

gromwell: taking 0.2 part of crushed lithospermum, adding 85% ethanol solution according to the material-liquid ratio of 1:14, carrying out ultrasonic extraction for 10min at the temperature of 85 ℃, filtering after extraction, taking supernate, carrying out rotary evaporation to dryness, then adding 30 parts of ethanol for redissolving, and diluting with 165 parts of pure water to prepare lithospermum extracting solution;

sixthly, the Chinese ephedra: taking 0.2 part of the crushed ephedra, adding 0.70mol/L hydrochloric acid solution according to the ratio of material to liquid of 1:20, stirring and leaching for 2.5h at the temperature of 80 ℃, repeatedly extracting for 4 times, filtering after extraction is finished, taking supernate, rotating and evaporating to dryness, then adding 30 parts of ethanol for redissolution, and diluting by using 165 parts of pure water to prepare an ephedra extract;

seventhly, fine leaf ledum palustre: taking 0.2 part of the crushed ledum minutissima, adding an absolute ethanol solution according to the material-liquid ratio of 1:25, stirring and extracting for 3 hours at the temperature of 70 ℃, filtering after extraction, taking supernate, carrying out rotary evaporation to dryness, then adding 30 parts of ethanol for redissolving, and diluting by using 165 parts of pure water to prepare the ledum minutissima extract.

(3) Compounding the Chinese herbal medicine extracting solution: mixing the single Chinese herbal medicine extracting solutions in equal proportion, uniformly stirring, and preparing a mixed solution to obtain a Chinese herbal medicine extracting solution;

(4) preparing a hand sanitizer: adding 30 parts of the Chinese herbal medicine extracting solution obtained in the step (3) and 70 parts of pure water into a container, stirring and mixing uniformly, then adding 0.8 part of SDS and 1.5 parts of glycerol, and stirring until the mixture is uniformly dispersed; and dissolving 3 parts of sodium chloride by using a small amount of pure water, adding into a container while stirring, adjusting the pH of the solution to 4.5-6.5 by using citric acid, finally adding 0.05 part of essence, and uniformly stirring and mixing to obtain the Chinese herbal medicine bacteriostatic agent hand sanitizer.

Example 3

A Chinese herbal medicine bacteriostatic agent is prepared by the following steps:

(1) pretreatment of Chinese herbal medicines: removing impurities from flos Caryophylli, radix et rhizoma Rhei, cortex Cinnamomi, Coptidis rhizoma, radix Arnebiae, herba Ephedrae, and Ledum Palustre L, cutting into 1-2cm segments, pulverizing, sieving with 60 mesh sieve, and collecting the undersize product;

(2) preparing a Chinese herbal medicine extracting solution: preparing various Chinese herbal medicines into extracting solutions according to the following extracting processes respectively;

firstly, clove: taking 1.5 parts of crushed clove, adding 75% ethanol solution according to the ratio of material to liquid of 1:15, carrying out ultrasonic extraction for 45min at the temperature of 45 ℃, filtering after extraction, taking supernate, carrying out rotary evaporation to dryness, then adding 25 parts of ethanol for redissolving, and diluting with 168 parts of pure water to prepare a clove extracting solution;

② rhubarb: taking 0.6 part of crushed rhubarb, adding 80% ethanol solution according to the ratio of material to liquid of 1:45, carrying out ultrasonic extraction for 25min at the temperature of 90 ℃, filtering after the extraction is finished, taking supernate, carrying out rotary evaporation to dryness, then adding 25 parts of ethanol for redissolving, and diluting with 168 parts of pure water to prepare rhubarb extract;

③ cinnamon: taking 0.4 part of crushed cinnamon, adding an acetone solution according to the material-to-liquid ratio of 1:40, carrying out ultrasonic extraction for 23min at the temperature of 40 ℃, filtering after extraction is finished, taking supernate, carrying out rotary evaporation to dryness, then adding 25 parts of ethanol for redissolving, and diluting with 168 parts of pure water to prepare a cinnamon extracting solution;

fourthly, coptis: taking 1 part of the crushed coptis, adding 65% ethanol solution according to the material-liquid ratio of 1:22, carrying out ultrasonic extraction for 80min at the temperature of 75 ℃, filtering after extraction, taking supernate, carrying out rotary evaporation to dryness, then adding 25 parts of ethanol for redissolving, and diluting with 168 parts of pure water to prepare rhubarb extract;

gromwell: taking 0.2 part of crushed lithospermum, adding 90% ethanol solution according to the material-liquid ratio of 1:13, carrying out ultrasonic extraction for 9min at the temperature of 80 ℃, filtering after extraction, taking supernate, carrying out rotary evaporation to dryness, then adding 25 parts of ethanol for redissolving, and diluting with 168 parts of pure water to prepare lithospermum extracting solution;

sixthly, the Chinese ephedra: taking 0.3 part of the crushed ephedra, adding 0.65mol/L hydrochloric acid solution according to the ratio of material to liquid of 1:15, stirring and leaching for 2h at the temperature of 75 ℃, repeatedly extracting for 3 times, filtering after the extraction is finished, taking supernate, rotating and evaporating to dryness, then adding 25 parts of ethanol for redissolution, and diluting with 168 parts of pure water to prepare an ephedra extract;

seventhly, fine leaf ledum palustre: taking 0.2 part of the crushed ledum minutissima, adding an absolute ethanol solution according to the material-liquid ratio of 1:23, stirring and extracting for 3.5 hours at the temperature of 70 ℃, filtering after extraction is finished, taking supernate, carrying out rotary evaporation to dryness, then adding 25 parts of ethanol for redissolution, and diluting with 168 parts of pure water to prepare the ledum minutissima extracting solution.

(3) Compounding the Chinese herbal medicine extracting solution: mixing the extractive solutions according to a certain proportion, stirring uniformly, and preparing into mixed solution to obtain Chinese herbal medicine extractive solution;

(4) preparing a hand sanitizer: adding 25 parts of the Chinese herbal medicine extracting solution obtained in the step (3) and 72 parts of pure water into a container, stirring and mixing uniformly, then adding 1 part of SDS and 1 part of glycerol, and stirring until the mixture is uniformly dispersed; and dissolving 2 parts of sodium chloride by using a small amount of pure water, adding the solution into a container while stirring, adjusting the pH of the solution to 4.5-6.5 by using citric acid, finally adding 0.05 part of essence, and uniformly stirring and mixing to obtain the Chinese herbal medicine bacteriostatic hand sanitizer.

Comparative example 1

The method of example 3 is followed to prepare a herbal bacteriostatic agent, except that the herbal is extracted by a mixed extraction method to obtain an extract, the mixed extraction steps are as follows:

taking 1.5 parts of crushed clove, 0.6 part of rhubarb, 0.4 part of cinnamon, 1 part of coptis chinensis, 0.2 part of lithospermum, 0.3 part of ephedra and 0.2 part of ledum tenuifolia, adding 50% ethanol solution according to the ratio of material to liquid of 1:40, carrying out ultrasonic extraction for 30min under the condition of 50 ℃, filtering after the extraction is finished, taking supernate, carrying out rotary evaporation to dryness, then adding 105 parts of ethanol for redissolution, and diluting with 706 parts of pure water to prepare mixed extracting solution.

Comparative example 2

The herbal bacteriostatic agent was prepared according to the method of example 3, except that the bacteriostatic agent was not added with the herbal extract, but was replaced with pure water.

Comparative example 3

The method of example 3 is followed to prepare a herbal bacteriostatic agent, except that the same amount of forsythia suspense extract is added to the coptis chinensis extract in the bacteriostatic agent, and the extraction process of the forsythia suspense extract is the same as that of coptis chinensis.

Examples of the experiments

The traditional Chinese medicine bacteriostatic hand sanitizer in the examples 1-3 and the comparative examples 1-3 is subjected to detection and analysis, and four indexes of decontamination rate, bacteriostasis, toxicology experiment and skin irritation are respectively detected, wherein the decontamination rate is measured according to a method for measuring the decontamination in a hand dishwashing detergent (GB 9985 & lt 2000 & gt), the bacteriostasis is measured according to a method specified in sanitation supervision and administration No. 2002 No. 229 & technical Specification for Disinfection (2002 edition), and the toxicology experiment and the skin irritation are measured according to a method specified in sanitation supervision and administration No. 2007 & No. 1 & lt sanitation Specification for cosmetics (2007 edition), and the measurement results are as follows:

TABLE 1 index test results of Chinese herbal medicine bacteriostatic agent

According to the above examples and experimental examples, it can be seen that the Chinese herbal medicine bacteriostatic agent in examples 1-3 has a good bacteriostatic effect on various pathogenic bacteria, and is high in decontamination rate, mild and non-irritant, wherein the Chinese herbal medicine bacteriostatic agent in example 3 has stronger decontamination capability and bacteriostatic performance, and is the best example of the invention, and when the components in the formula are changed, the bacteriostatic effect of the Chinese herbal medicine bacteriostatic agent is significantly affected.

The above description is only for the preferred embodiment of the present invention, and is not intended to limit the scope of the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.