阅读说明：本技术 环境压力诱发物质测定装置 (Ambient pressure-induced substance measuring device ) 是由 崔允硕 张须净 李承玟 许承 于 2019-12-06 设计创作，主要内容包括：根据本发明的一实施例,公开一种能够分析水产养殖场的水质状态的环境压力诱发物质测定装置,其特征在于,包括：试剂供应部,通过试剂供应路径向传感器单元定量供应发光试剂；样品定量供应部,通过样品供应路径接收样品试料而向传感器单元定量供应样品试料；以及传感器单元,从所述试剂供应部和所述样品定量供应部接收所述发光试剂和所述样品试料,所述传感器单元包括：流通单元(flow cell),提供能够使所述发光试剂和所述样品试料彼此混合而进行化学发光反应的空间；以及光检测部,能够感测通过所述化学发光反应发出的光。(According to an embodiment of the present invention, there is provided an environmental pressure-inducing substance measurement device capable of analyzing a water quality state of an aquaculture farm, the device including: a reagent supply section for quantitatively supplying a light-emitting reagent to the sensor unit through the reagent supply path; a sample quantitative supply unit that receives a sample through the sample supply path and quantitatively supplies the sample to the sensor unit; and a sensor unit that receives the luminescent reagent and the sample from the reagent supply unit and the sample quantitative supply unit, the sensor unit including: a flow cell (flow cell) that provides a space in which the luminescent reagent and the sample specimen can be mixed with each other to perform a chemiluminescent reaction; and a light detection part capable of sensing light emitted by the chemiluminescent reaction.)

1. An environmental pressure-inducing substance measurement device (150) capable of analyzing a water quality state of an aquaculture farm, comprising:

a reagent supply unit (151) for quantitatively supplying a luminescent reagent to the sensor unit (159) through the reagent supply path;

a sample quantitative supply unit (157) that receives the sample through the sample supply path and quantitatively supplies the sample to the sensor unit (159); and

a sensor unit (159) that receives the luminescent reagent and the sample from the reagent supply unit (151) and the sample quantitative supply unit (157),

the sensor unit (159) includes:

a flow cell (152) that provides a space in which the luminescent reagent and the sample specimen can be mixed with each other to cause a chemiluminescent reaction; and

and a light detection section (154) capable of sensing light emitted by the chemiluminescent reaction.

2. The environmental pressure-inducing substance measuring apparatus according to claim 1,

the reagent supply section (151) includes:

a first reagent supply unit (151a) for supplying a first reagent;

a second reagent supply unit (151b) for supplying a second reagent; and

a third reagent supply part (151c) for supplying a third reagent,

wherein the first agent is a compound capable of chemiluminescence, the second agent or the third agent is an active agent that activates chemiluminescence of the first agent,

the first reagent supply section supplies the first reagent to the sensor unit (159) at a first fixed amount, the second reagent supply section supplies the second reagent to the sensor unit (159) at a second fixed amount, and the third reagent supply section supplies the third reagent to the sample fixed amount supply section (157) at a third fixed amount.

3. The environmental pressure-inducing substance measuring apparatus according to claim 2,

the second reagent is one of an oxidizing agent and a reducing agent,

the third agent is one of a basic compound and an acidic compound.

4. The environmental pressure-inducing substance measuring apparatus according to claim 1,

the light detection section (154) includes a converter that converts the intensity of the sensed light into an electric signal and outputs the electric signal,

the converter is a photomultiplier tube, a photodiode, or a phototransistor.

5. The environmental pressure-inducing substance measuring device according to claim 1, further comprising:

a filter section (155) located upstream of the sample quantitative supply section (157) on the sample supply path,

the filter unit (155) receives the sample and filters the sample, and the filtered sample is supplied to the sample quantitative supply unit (157).

6. The environmental pressure-inducing substance measuring apparatus according to claim 5,

the filter section (155) is composed of an adsorptive resin column containing a masking agent,

an interfering substance that interferes with a chemiluminescent reaction between the luminescent reagent and the sample is filtered by the masking agent.

7. The environmental pressure-inducing substance measuring apparatus according to claim 1,

the flow cell (152) is composed of a reaction chamber (152a), the reaction chamber (152a) has an internal space capable of accommodating a luminescent reagent and a sample and maintaining airtightness,

the reaction chamber (152a) is formed by using a material, and the other part except for a part of the reaction chamber (152a) as a light transmission part which is not light-transmitted is surrounded by an opaque material, so that the light emitted from the internal space can be transmitted only through the part,

the light detection part (154) is formed with a light inlet hole capable of receiving the light transmitted through the light transmission part of the reaction chamber (152a),

the light inlet hole is formed in such a size as to completely cover the light transmitting portion of the reaction chamber (152a) so that the light flowing out of the light transmitting portion is transmitted only to the light detecting portion (154),

the light inlet hole and the light transmitting portion are configured so that the flow cell (152) and the light detecting portion (154) are joined in close air-tight contact at corresponding positions.

8. The environmental pressure-inducing substance measuring apparatus according to claim 1,

the sample quantitative supply section (157) is constituted by a six-way valve (157) formed so as to be able to change a flow path.

9. The environmental pressure-inducing substance measuring apparatus according to claim 1,

the ambient pressure-inducing substance is selected from the group consisting of,

manganese oxide, ferric oxide, uric acid, cobalt, glutamic acid, ascorbic acid, lactam antibiotics and marine phaeocystis.

10. The environmental pressure-inducing substance measuring apparatus according to claim 2,

the first reagent is any one of luminol, lucigenin, luciferin, acridine, oxalic acid and ruthenium.

11. The environmental pressure-inducing substance measuring apparatus according to claim 6,

the masking agent is any one of ferric oxide, ascorbic acid and perylene.

12. The environmental pressure-inducing substance measuring device according to claim 1, further comprising:

an optical wavelength separator (156) capable of separating light emitted from the flow cell (152) by the chemiluminescence reaction into light of different wavelengths from each other, an

A plurality of light detection units (154),

each of the plurality of light detection units (154) individually receives the light of each wavelength separated by the optical wavelength separator (156) and detects the light.

13. The environmental pressure-inducing substance measuring device according to claim 5, further comprising:

a plurality of tube-type linkage pumps (153),

the plurality of tube-type pumps (153) are configured to be arranged upstream of the filter section (155) on the sample supply path and downstream of the reagent supply section (151) on the reagent supply path,

a tube-type interlock pump (153a) disposed upstream of the filter unit (155) on the sample supply path is connected to a sample storage unit in which a sample is stored to supply a sample to the filter unit (155) or the sample quantitative supply unit (157),

tube-type pumps (153b, 153d) arranged downstream of the reagent supply section (151) on the reagent supply path are configured to supply the luminescence reagent stored in the reagent supply section (151) to the sensor unit (159).

Technical Field

The present invention relates to an ambient pressure-inducing substance measuring apparatus, and more particularly, to an ambient pressure-inducing substance measuring apparatus capable of analyzing a water quality state of an aquaculture farm.

Background

The environmental stress represents a stress due to a lost feed or a stress due to a secreted substance of fish and shellfish, etc., as a factor that hinders the growth of farmed organisms in an aquaculture farm and may further destroy the ecological environment.

Such an increase in the environmental pressure-inducing substances in the aquaculture farm leads to a large number of deaths of the cultured organisms in the farm, and causes problems in that the ecological environment is destroyed by the water discharged from the farm to the outside.

Recently, in order to prepare a timely countermeasure against water pollution while minimizing water pollution, the settings of water pollution measuring and monitoring systems for measuring the state of water quality in real time are increasing.

As disclosed in patent document 1, which is a prior art, there is a system for monitoring water pollution using an optical sensor. However, the water quality information that can be measured by the optical sensor used for this technique is only of a degree that is easy to measure by domestic techniques such as hydrogen ion concentration (pH), oxidation-reduction potential (ORP), oxygen dissolved amount (DO), or Sodium (Sodium).

Due to, for example, NH₄、NO^2-Or NO^3-The water quality information is generally obtained by using products to which expensive foreign technologies are applied, resulting in complicated purchase and flow processes and requiring a large amount of maintenance costs.

The prior art in patent document 1 also has a problem that contaminants accumulate around the sensor to degrade the accuracy and precision of the sensor, and in order to solve the problem, a physical cleaning method is disclosed in which periodic cleaning is performed using a brush or the like arranged around the sensor. However, the periodic cleaning device may not be sufficiently cleaned during a period of serious contamination, so that there may be a problem in that the accuracy and precision of the water quality measurement are lowered.

[ Prior art documents ]

[ patent document ]

Patent document 1: korean laid-open patent No. 2005-108734

Disclosure of Invention

According to an embodiment of the present invention, there can be provided an environmental pressure-inducing substance measuring apparatus capable of measuring organic contaminants and red tide phytoplankton (Chattonlella marina) causing red tide, which are contained in seawater of fish or shellfish farms, such as manganese oxide (e.g., Mn (II)), iron oxide (e.g., Fe (II)), uric acid (uric acid), cobalt (Co), Glutamic acid (Glutamic acid), Ascorbic acid (Ascorbic acid), lactam antibiotics (L actam antibiotics), or seawater, and the like, so as to be suitable for analyzing the water quality state of aquaculture farms.

According to an embodiment of the present invention, it is possible to provide an environmental pressure-inducing substance measuring apparatus which is easy to use and maintain and does not incur a cost burden.

Also, according to an embodiment of the present invention, it is possible to provide an environmental pressure-induced substance measurement apparatus capable of improving accuracy and precision of a sensor cell by filtering a sample before the sample is supplied to the sensor cell.

According to an embodiment of the present invention, there is provided an environmental pressure-inducing substance measuring apparatus capable of analyzing a water quality state of an aquaculture farm, including: a reagent supply section for quantitatively supplying a light-emitting reagent to the sensor unit through the reagent supply path; a sample quantitative supply unit that receives a sample through the sample supply path and quantitatively supplies the sample to the sensor unit; and a sensor unit that receives the luminescent reagent and the sample from a reagent supply unit and a sample quantitative supply unit, the sensor unit including: a flow cell (flow cell) that provides a space in which the luminescent reagent and the sample specimen can be mixed with each other to perform a chemiluminescent reaction; and a light detection part capable of sensing light emitted by the chemiluminescent reaction.

In the above embodiment, the reagent supply part may include: a first reagent supply unit for supplying a first reagent; a second reagent supply unit for supplying a second reagent; and a third reagent supply unit for supplying a third reagent.

In the above embodiment, the first reagent may be a compound capable of chemiluminescence, and the second reagent or the third reagent may be an active agent that activates chemiluminescence of the first reagent.

In the above embodiment, the first reagent supplying portion may supply the first reagent to the sensor unit at a first fixed amount, the second reagent supplying portion may supply the second reagent to the sensor unit at a second fixed amount, and the third reagent supplying portion may supply the third reagent to the sample fixed amount supplying portion at a third fixed amount.

The environmental pressure-inducing substance measurement apparatus according to an embodiment of the present invention may further include: a filter section located upstream of the sample quantitative supply section on the sample supply path.

In the above embodiment, the filter unit may receive the sample and filter the sample, and the filtered treated water may be supplied to the sample quantitative supply unit.

Also, in the above-described embodiment, the filter part may be constituted by an absorbent resin column (resin column) including a Masking agent (Masking agent) by which an interfering substance that interferes with a chemiluminescent reaction of the luminescent reagent and the sample specimen may be filtered.

The sensor unit constituting the environmental pressure-inducing substance measurement device of the present invention has an effect of being easy to use and manage with a simple configuration.

The sensor unit according to an embodiment of the present invention is composed of a chemiluminescence sensor (chemiluminiscence sensor), and can provide an apparatus for measuring an environmental pressure-inducing substance, which is used for measuring manganese oxide (for example, mn (ii)), iron oxide (for example, fe (ii)), Uric acid (Uric acid), cobalt (Co), Glutamic acid (Glutamic acid), Ascorbic acid (Ascorbic acid), lactam antibiotics (L actam antibiotics), organic pollutants in seawater, red tide phytoplankton (Chatton Phaeophyta) causing red tide, and the like, included in seawater of a fish or shellfish farm.

In particular, since the substance that interferes with the measurement of the environmental pressure-inducing substance is marked (targeting) and removed by the Masking agent (Masking agent) included in the filter unit before being supplied to the sensor unit, the measurement can be performed more accurately.

Drawings

FIG. 1 is a view for explaining the configuration of an ambient pressure-inducing substance measurement apparatus according to an embodiment of the present invention,

FIG. 2 is a view for explaining a sample quantitative supply portion according to an embodiment of the present invention,

fig. 3 is a diagram for explaining an exemplary structure of a sensor unit according to an embodiment of the present invention,

FIG. 4 is a diagram for explaining a structure of a sensor unit according to another embodiment of the present invention, and

fig. 5 is a table for explaining Masking agents (Masking agents) according to light emitting agents and interfering substances according to an embodiment of the present invention.

Description of the symbols

150: ambient pressure-inducing substance measurement device 151: reagent supply part

152: flow cell (flow cell) 153: pipe type linkage pump

154: light detection unit 155: filter part

157: sample quantitative supply section 159: sensor unit

161: t-pipe (T-piece) 200: computer with a memory card

201: amplifying section 203: AD converter

205: computer processor

Detailed Description

The above objects, other objects, features and advantages of the present invention can be easily understood by the accompanying drawings and the related preferred embodiments below. However, the present invention is not limited to the embodiments described herein, and may be embodied in other forms. Rather, the embodiments described herein are provided so that this disclosure will be thorough and complete, and will fully convey the concept of the invention to those skilled in the art.

In the present specification, when a component is referred to as being located above another component, it means that the component may be directly formed on the another component or a third component may be interposed therebetween. In the drawings, the thicknesses of the constituent elements are exaggerated to effectively explain the technical contents.

In the case where the terms first, second, etc. are used in the present specification to describe the constituent elements, these constituent elements should not be limited by these terms. These terms are only used to distinguish one constituent element from another constituent element. The embodiments illustrated and described herein include complementary embodiments thereto.

In this specification, the singular forms include plural unless specifically mentioned herein. The constituent element referred to as "comprising" and/or "including" used in the specification does not exclude the presence or addition of one or more other constituent elements.

In the present specification, "and/or" means "and" or ", for example," including component a and/or component B "is used to mean including component a, component B, or including component a and component B (that is, including at least one of component a and component B).

Definition of terms

In the present embodiment, the phrase that the component a is located "upstream" of the component B in the path in which the fluid flows means that the fluid passes through the component B after passing through the component a first by arranging the component a and the component B in the path or by constituting in the path.

In the present specification, the reference that the component B is located "downstream" of the component a on a path in which the fluid flows means that the fluid passes through the component B after passing through the component a first by arranging the component a and the component B on the path or constituting the path.

In the present specification, the reference that the component a is located on a certain "path" means that the component a and the path are organically arranged or organically constructed with each other so that the component a receives the fluid flowing in the path or the component a causes the fluid flowing in the path to flow out.

In the present specification, a "path" or a "pipe" provides a space to allow a fluid to flow, and for example, may be a pipe or the like having an inner space closed in such a manner as not to move the fluid by leakage.

In this specification, "communication" means a case where paths or pipes are connected in such a manner that a fluid can move, and "non-communication" means a case where paths or pipes are not connected to each other in such a manner that a fluid cannot move.

In the present specification, the "environmental stress-inducing substance" may include, as contaminants that may destroy the ecological environment of an aquaculture farm, manganese oxide (e.g., mn (ii)), iron oxide (e.g., fe (ii)), Uric acid (Uric acid), cobalt (Co), Glutamic acid (Glutamic acid), Ascorbic acid (Ascorbic acid), lactam antibiotics (L actam antibiotics), and/or organic contaminants in seawater, red tide phytoplankton (Chatton) (Chattonella marina), and the like, which are contained in seawater of the aquaculture farm.

The present invention is described in detail below with reference to the accompanying drawings. In describing the following specific embodiments, numerous specific details are set forth in order to provide a more thorough understanding of the invention. However, those skilled in the art, having the degree of knowledge that the present invention can be understood, will recognize that many of the specific details described above may be employed without one skilled in the art. It is previously stated that in some cases, in order to prevent confusion in describing the present invention, no description is given of parts which are already known and which are not too much relevant to the invention in describing the present invention.

As shown in fig. 1, an ambient pressure-inducing substance measurement apparatus 150 according to an embodiment of the present invention includes: a reagent supply unit 151 for quantitatively supplying a luminescent reagent to the sensor unit 159; the sample quantitative supply unit 157 quantitatively supplies the sample to the sensor unit 159.

When the sample contains an environmental pressure-inducing substance detectable by the luminescent reagent, the presence or absence of the environmental pressure-inducing substance can be known by luminescence from a chemiluminescent reaction between the luminescent reagent and the environmental pressure-inducing substance. Further, according to embodiments, the amount of the ambient pressure inducing substance may also be known.

The chemiluminescence reaction between the luminescent reagent and the detection target is a phenomenon in which an electrically excited product is generated, and the product directly emits light while returning from an excited state to a ground state, or emits light by transferring energy of the excited state to another molecule.

Such a chemiluminescent reaction is usually slow, and thus is difficult to visually confirm in a short time. Therefore, the reaction can be promoted by using an appropriate catalyst in the luminescent reagent, and in the present invention, the detectable environmental pressure-inducing substance plays such a catalytic role, and the "detection object" in the present invention means the environmental pressure-inducing substance included in the sample.

In the following embodiments of the present invention, a specific configuration of the environmental pressure-inducing substance measuring device 150 capable of detecting the above-described environmental pressure-inducing substance will be described.

In the present specification, the "luminescent reagent" that emits light when reacting with a detection target is a compound that can undergo a chemiluminescent reaction, such as luminol (L luminol), and is used in a meaning including both "luminescent reagent in a narrow sense" and "luminescent reagent in a broad sense" that is a mixture that can undergo a chemiluminescent reaction by mixing a plurality of compounds.

Fig. 1 is a diagram for explaining the configuration of an ambient pressure-inducing substance measurement apparatus according to an embodiment of the present invention.

Referring to fig. 1, an ambient pressure-inducing substance measurement device 150 according to an embodiment may include a reagent supply unit 151, a plurality of tube-type pumps 153, a filter unit 155, a sample quantitative supply unit 157, and a sensor unit 159. Here, the reagent supply unit 151 and a part of the tube-type interlock pump 153 are located on the reagent supply path, and the reagent supply unit 151 is located upstream of the tube-type interlock pump 153. Also, the filter section 155 and the sample quantitative supply section 157 may be located on the sample supply path, with the filter section 155 located upstream of the sample quantitative supply section 157. Further, the sensor unit 159 is located at a point where the sample supply path and the reagent supply path meet each other.

The sensor unit 159 receives a quantitative amount of the luminescent reagent from the reagent supply portion 151 and a quantitative amount of the sample from the sample quantitative supply portion 157, thereby having a configuration in which such luminescent reagent and sample are mixed, and senses light from the mixed luminescent reagent and sample (hereinafter, referred to as "mixture"), thereby making it possible to sense an object of detection included in the sample.

According to an embodiment, the reagent supply portion 151 stores a luminescent reagent and quantitatively supplies the luminescent reagent to the sensor unit 159 through a reagent supply path.

In the embodiment of the present invention, the "reagent supply path" represents a component providing a space in which the luminescent reagent stored in the reagent supply portion 151 can move. For example, the fluid may be a pipe or the like having an inner space closed so as not to move the fluid.

According to an embodiment, the tube-type interlock pumps 153a, 153b, 153c may be disposed downstream of the reagent supply portion 151 on the reagent supply path, so that the reagent may be sucked from the reagent supply portion 151 to be supplied to the sensor unit 159 side, and the supply amount of the supplied luminescence reagent may be controlled.

For example, the tube-type interlock pumps 153a, 153b, 153c may supply a predetermined amount (hereinafter, referred to as "fixed amount") of the luminescent reagent from the reagent supply portion 151 to the sensor unit 159 for a predetermined time.

According to the present embodiment, the reagent supply unit 151 may be configured in plural. As shown in fig. 2, the reagent supply part 151 may include: a first reagent supply unit 151a for supplying a first reagent R1; a second reagent supply unit 151b for supplying a second reagent R2; and a third reagent supply unit 151c for supplying a third reagent R3. The first reagent supply portion 151a may be configured to supply the first reagent R1 at a first amount to the sensor unit 159; the second reagent supply unit 151b may be configured to supply the second reagent R2 at a second constant amount to the sensor unit 159; the third reagent supplying portion 151c may be configured to supply the third reagent R3 to the sample quantitative supply portion 157 at a third quantitative amount.

According to the present embodiment, the respective tube-type interlock pumps 153a, 153b, 153c are disposed on the respective reagent supply paths connected to the respective reagent supply portions 151a, 151b, 151c to control the amounts of reagents supplied from the respective reagent supply portions 151a, 151b, 151 c.

According to the present embodiment, the environmental pressure-inducing substance measurement device 150 according to an embodiment may further include a T-shaped pipe 161 located on the reagent supply path. The T-pipe 161 has a configuration that at least two fluids can be input and the received two fluids are mixed and output. T-pipe 161 is located downstream of tubing gang pumps 153a, 153b and upstream of sensor unit 159.

Further, the T-shaped pipe 161 may receive the output of the pipe-type interlock pump 151a and the output of the pipe-type interlock pump 151b to mix and output them with each other. The output of the T-pipe 161 is supplied to a sensor unit 159.

For example, as shown in fig. 1, the respective tube-type pumps 153a and 153b connected to the respective reagent supply units 151a and 151b via the T-shaped pipe 161 are connected, so that the reagents R1 and R2 output by the tube-type pumps 153a and 153b can be supplied to the sensor unit 159 in a mixed state. The reagent R3 output by the tube-type pump 151c can be supplied to the sample quantitative supply portion 157.

However, it is merely exemplary, and may be configured to mix only necessary reagents.

According to an embodiment, the first reagent R1, the second reagent R2, and the third reagent R3 are reagents having different functions, respectively, and the reagent supplied to the sensor unit 159 may be configured to include a mixture of one or more of the first reagent R1, the second reagent R2, or the third reagent R3. For example, the luminescence reagent supplied from the reagent supplying part 151 to the sensor unit 159 may be composed of a mixture including one or more of the first reagent R1 or the second reagent R2, and the luminescence reagent supplied from the reagent supplying part 151 to the sample quantifying part 157 may be composed of the third reagent R3.

According to an embodiment, the first reagent R1 is a luminescent reagent in a narrow sense as a compound that can be chemiluminescent, and the second reagent R2 and the third reagent R3 are composed of an activator that activates chemiluminescence of the first reagent R1.

According to an embodiment, the second reagent R2 may be composed of one of an oxidizing agent or a reducing agent that produces a chemiluminescent reaction with the first reagent R1 to make the first reagent R1 chemiluminescent.

According to an embodiment, the third reagent R3 may be composed of a basic compound or an acidic compound that produces a chemiluminescent reaction with the first reagent R1 to make the first reagent R1 chemiluminescent. For efficient reaction, it is preferably composed of a strongly basic compound or a strongly acidic compound. Further, the third reagent R3 may be supplied to the sample quantitative supply portion 157 to function as a carrier within the sample quantitative supply portion 157.

For example, any one of luminol (L uinol), lucigenin (L ucigenin), luciferin (L uciferin), Acridinium (Acridinium), oxalic acid (Oxalate), or ruthenium (ruthenium) may be used.

The sample quantitative supply portion 157 may receive a sample through a sample supply path and quantitatively supply the sample to the sensor unit 159.

The "sample supply path" is a component providing a space in which the sample received by the sample quantitative supply unit 157 can move. For example, the fluid may be a pipe or the like having an inner space closed so that the fluid can move without leaking.

According to an embodiment, the sample quantitative supply portion 157 may receive the sample specimen from the sample storage portion R disposed on the sample supply path. The tube-type interlock pump 153d may be disposed on the sample supply path in such a manner as to be connected to the sample storage portion R to suck the sample stored in the sample storage portion R to be supplied to the filter portion 155, and may control the supply amount of the sample supplied from the sample storage portion R to the filter portion 155.

According to the present embodiment, the ambient pressure-inducing substance measurement device 150 according to an embodiment may further include a filter unit 155 located on the sample supply path.

The filter portion 155 may be located upstream of the sample quantitative supply portion 157 on the sample supply path. The filter unit 155 filters the sample received from the sample storage unit R, and supplies the filtered sample (treated water) to the sample quantitative supply unit 157.

According to an embodiment, the filter part 155 may be composed of an absorbent resin column (resin column) including a Masking agent (Masking agent) or an extraction coil (extraction coil). The Masking agent (Masking agent) is a substance for filtering an interfering substance in the sample, which interferes with the chemiluminescent reaction between the substance to be detected and the luminescent reagent.

The filter unit 155 may remove an interfering substance in the sample with a Masking agent (Masking agent) or may perform filtering so as to extract only a substance to be detected.

A plurality of luminescent reagents may be used according to the kind of the environmental pressure-inducing substance to be detected, and the kind of the interfering substance that interferes with the reaction may be different depending on the luminescent reagent. The Masking agent (Masking agent) can be variously configured to be capable of labeling (targeting) and removing an interfering substance by the luminescent reagent, and thus more accurate and precise detection can be performed.

FIG. 5 is a table for explaining a luminescent reagent, an interfering substance, and a masking agent (Maskingagent) according to an object of detection. A specific example is explained below with reference to fig. 5.

Example 1) case where the object to be detected is Uric acid (Uric acid)

In order to detect Uric acid (Uric acid) corresponding to an environmental pressure-inducing substance in a sample, Octyl phenyl Polyglycol ether (Octyl phenyl Polyglycol ether) may be used as the first reagent R1, and potassium permanganate (KMnO) may be used as the luminescent reagent₄) As a second reagent R2, nitric acid (HNO)₃) Used as the third reagent R3. Interfere the chemiluminescent reaction of the luminescent reagent and uric acid (Uricacid)The substance of (a) is Ascorbic acid (Ascorbic acid), and ferric ion (fe (iii)) which is one kind of iron oxide can be used as a Masking agent (Masking agent) therefor.

Example 2) case where the object to be detected is divalent cobalt ion (Co (II))

In order to detect divalent cobalt ions (co (ii)) corresponding to the environmental pressure-inducing substance in the sample, luminol (L mininol) may be used as the first reagent R1, and hydrogen peroxide (H) may be used as the luminescent reagent₂O₂) As the second reagent R2, sodium hydroxide (NaOH) was used as the third reagent R3. The substance interfering with the chemiluminescent reaction of the luminescent reagent with the divalent cobalt ion (Co (II)) is ferric ion (Fe (III)), and Ascorbic acid (Ascorbic acid) can be used as a Masking agent (Masking agent) for this.

Example 3) case where the object to be detected was 1-glutamic acid (1-glutamic acid)

In order to detect 1-glutamic acid (1-glutamic acid) corresponding to an environmental stress-inducing substance in a sample, peroxyoxalate (peroxixlate) can be used as the first reagent R1 and hydrogen peroxide (H) can be used as the luminescent reagent₂O₂) As a second reagent R2, nitric acid (HNO)₃) Used as the third reagent R3. Substances interfering with the chemiluminescent reaction of the luminescent reagent with 1-glutamic acid (1-glutamic acid) are monovalent potassium ions (K (I)) and divalent magnesium ions (Mg (II)), for which Perylene (Perylene) can be used as a Masking agent (Masking agent).

The operation of the sample quantitative supply portion 157 will be described in detail below with reference to fig. 2.

Referring to fig. 2, the sample quantitative supply portion 157 is configured to supply a quantitative amount of a sample. For example, the six-way valve may be configured to be capable of quantitatively supplying the sample by changing the position a (position a) and the position b (position b). Hereinafter, the operation of the sample constant-volume supply unit 157 will be described assuming that the sample constant-volume supply unit 157 is constituted by a six-way valve.

The sample quantitative supply portion 157 includes a body B equipped with six ports P1, P2, P3, P4, P5, P6, into or out of which fluid can be input or output, and conduits L1, L2, L3, L4 here, the conduits L1, L2, L3, L4 are located outside the body B for communication between the ports or with an external path (or conduit).

The first tube L1 is a path for communicating the first port (or, also referred to as "sample-side port") P1 with a sample reagent path, specifically, an output of the filter section 155, the second tube L2 is a path for communicating the second port P2 with the fifth port P5, the third tube L3 is a path for communicating the third port (or, referred to as "sensor-side port") P3 with a sample reagent path, specifically, an input of the sensor unit 159, the fourth tube L4 is a path for communicating the fourth port with a carrier storage section (not shown), and the fifth tube L5 is a path for communicating the sixth port P6 with an exhaust section (not shown).

That is, the first tube L1 is used to input the sample output from the filter unit 155 to the first port P1, and the third tube L3 is used to input the sample output from the third port P3 to the sensor unit 159.

The pipes L1, L2, L3 and L4 do not always flow fluid, but are connected to ports in the body B such that a part of the pipes L1, L2, L3 and L4 flows fluid and the remaining part is blocked by no fluid flow.

In Position a, the body B is internally connected in such a manner that the first port P1 and the second port P2 communicate with each other, and is internally connected in such a manner that the fifth port P5 and the sixth port P6 communicate with each other. Here, the third port P3 and the second port P2 are not internally communicated with each other, and the fourth port P4 and the fifth port P5 are also not internally communicated with each other.

Further, for the purpose of explanation of the present invention, a case where the valve (not shown) is located at the Position that becomes Position A will be referred to as a case where the quantitative filling operation of the sample quantitative supply section 157 is performing the quantitative filling operation, which is an operation of quantitatively filling the sample, and in this embodiment, the sample may be filled into, for example, the second pipe L.

In Position B, the main body B is internally connected in such a manner that the first port P1 and the sixth port P6 communicate with each other, and the second port P2 and the third port P3 communicate with each other, and the fourth port P4 and the fifth port P5 communicate with each other. Here, the third port P3 and the fourth port P4 are not internally communicated with each other, and the fifth port P5 and the sixth port P6 are also not internally communicated with each other.

Further, for the purpose of explanation of the present invention, the case where the valve (not shown) is located at the Position that becomes Position B is referred to as the case where the sample constant-volume supply portion 157 is performing the constant-volume supply operation. That is, the constant-volume supply operation is an operation of supplying the sample that is quantitatively filled in the constant-volume filling operation to the sensor unit 159.

In the Position a state (i.e., the constant-volume filling state), the sample supplied to the first pipe L1 by the tube-type gang pump 153 is discharged to the outside through the first port P1, the second port P2, the second pipe L2, the fifth port P5, the sixth port P6, and the fifth pipe L5, and the carrier is supplied to the sensor unit 159 through the fourth pipe L4, the fourth port P4, the third port P3, and the third pipe L3.

Immediately after the sample is discharged to the outside through the fifth duct L5, the state is switched to the Position B state (i.e., the constant-volume-supply operation state).

If the Position a is changed to the Position B, the sample supplied to the first conduit L1 by the tube-type interlock pump 153 is discharged to the outside through the first port P1, the sixth port P6, and the fifth conduit L5, and if the carrier is supplied to the fourth conduit L4, the sample filled in the second conduit by the carrier is supplied to the sensor unit 159 through the third port P3, specifically, if the carrier is supplied to the fourth conduit L4, the carrier is supplied to the sensor unit 159 through the fourth port P4, the fifth port P5, the second conduit L2, the second port P2, the third port P3, and the third conduit L3.

Here, if all the sample stored in the third duct L3 is supplied to the sensor unit 159, it is thereafter switched to the Position a state (i.e., the quantitative filling operation state).

In the manner described above, the switching operation is alternately performed as the Position a and the Position B to supply the sample to the sensor unit 159 in a fixed amount.

Fig. 3 is a diagram for explaining an exemplary structure of a sensor unit according to an embodiment of the present invention.

Referring to fig. 3, the sensor unit 159 includes: a flow cell (flow cell)152 that provides a space in which a luminescent reagent and a sample can be mixed with each other to perform a chemiluminescent reaction; the light detecting part 154 may sense light emitted by a chemiluminescent reaction.

According to an embodiment, the sensor unit 159 may be manufactured in a form of mounting the flow cell (flow cell)152 and the light detecting part 154 on a substrate of a flexible material such as an aluminum plate (aluminum plate).

The flow cell (flow cell)152 may be constituted by a reaction chamber 152a capable of accommodating a luminescent reagent and a sample specimen and having an internal space maintained airtight. The reaction chamber 152a is formed using a transparent material that can transmit light, and the other portion (hereinafter, referred to as a "light-opaque portion") than a portion through which light emitted from the internal space can be transmitted only through a portion of the reaction chamber 152a (hereinafter, referred to as a "light-transmitting portion") is surrounded by an opaque material.

The reaction chamber 152a may be formed of transparent glass, such as borosilicate, or transparent plastic, such as acrylic plastic, which is excellent in durability and transmittance.

According to an embodiment, the reaction chamber 152a may be surrounded by a material such as black PVC, which is opaque, to form a portion other than the light transmitting portion allowing light to transmit therethrough so that light cannot leak.

The light detecting section 154 is coupled to the flow cell (flow cell)152 in such a manner as to completely cover the light transmitting section allowing light to transmit in the reaction chamber 152 a.

According to an embodiment, a light inflow hole PMT hole capable of receiving light transmitted through a light transmitting portion of the reaction chamber 152a may be formed at the light detecting portion 154, and hermetically coupled to the flow cell 152 at a position corresponding to the light transmitting portion of the reaction chamber 152 a. Here, the light inlet hole is preferably configured as a light transmitting portion closely attached and joined to the reaction chamber 152a in a size that can completely cover the light transmitting portion of the reaction chamber 152a so that light flowing out of the light transmitting portion of the reaction chamber 152a is transmitted only to the light detecting portion 154.

According to an embodiment, the light detecting part 154 may include a converter that converts the intensity of the sensed light into an electrical signal and outputs it if the light flowing out is detected at the light transmitting part of the reaction chamber 152 a. The converter may be constituted by, for example, a photomultiplier Tube (PMT), a photomultiplier, a photodiode, or a phototransistor (Photo transistor).

That is, the light detection section 154 is a device that can receive light, i.e., light rays, and convert the light rays into an electrical signal, and may further include an element that amplifies the light and/or the electrical signal.

The electric signal output from the light detection unit 154 is transmitted to the computer 200, and the computer 200 can analyze the characteristics of the electric signal to determine the degree of contamination of the sample.

For example, the computer 200 can know whether or not the sample contains the environmental pressure-inducing substance to be detected by comparing the intensity of the electric signal with a reference value (a value predetermined for the environmental pressure-inducing substance). The computer 200 can also determine the amount of the environmental pressure-inducing substance desired to be detected by analyzing how much the intensity of the electric signal is greater than the reference value.

The light-emitting reagent may be used in a variety of forms depending on the type of the environmental stress-inducing substance to be detected. Thus, the reference value may represent a value predetermined in accordance with the luminescent agent of the environmental pressure inducing substance.

Fig. 4 is a diagram for explaining the structure of a sensor unit according to another embodiment of the present invention.

Referring to fig. 4, the sensor unit 159 includes: a flow cell (flow cell)152 that provides a space in which a luminescent reagent and a sample can be mixed with each other to perform a chemiluminescent reaction; a plurality of light detecting portions 154a, 154b, 154c that can sense light emitted by a chemiluminescent reaction; and an optical wavelength separator 156.

Comparing the embodiment of fig. 4 with the embodiment described with reference to fig. 1 to 3, the embodiment of fig. 4 further includes an optical wavelength separator 156, and there is a difference in that the sensor unit 159 includes a plurality of optical monitoring portions. Hereinafter, the embodiment of fig. 4 will be described mainly in terms of differences.

According to the embodiment of fig. 4, the sensor unit 159 shown in fig. 4 is configured such that, when one or more compounds capable of chemiluminescence (a luminescence reagent in a narrow sense) is included in the luminescence reagent supplied from the reagent supply portion 151, a detection target according to the kind of the luminescence reagent can be confirmed.

Since the wavelength of the emitted light differs depending on the kind of the luminescent reagent, when a plurality of luminescent reagents are reacted in the flow cell (flowcell)152, light having different wavelengths is emitted.

The optical wavelength separator 156 is an optical element that receives optical signals including light having different wavelengths from each other and separates the light according to the wavelengths.

According to one embodiment, the optical wavelength separator 156 receives light emitted from the flow cell 152 and separates the light into wavelengths different from each other.

The optical wavelength separator 156 and the flow cell 152 are coupled so that the optical wavelength separator 156 does not allow the light emitted from the flow cell 152 to flow out to the outside but allows the light to flow into the optical wavelength separator 156.

The light having different wavelengths separated from the optical wavelength separator 156 is supplied to the plurality of light detectors 154a, 154b, and 154c, respectively.

Each of the light detectors 154a, 154b, and 154c is configured to be capable of independently receiving light of each wavelength separated by the optical wavelength separator 156 and detecting the light, and thereby can check a detection target according to the type of the luminescent reagent.

The operation and configuration of each of the plurality of light detection units 154a, 154b, and 154c in fig. 4 can be described with reference to fig. 1 to 3.

As described above, since it is understood that various modifications and variations can be made from the description in the above description by those having ordinary knowledge in the field to which the present invention pertains, the scope of the present invention should not be limited to the described embodiments, but should be defined not only by the scope of the claims but also by the scope equivalent to the scope of the claims.