阅读说明：本技术 一种地黄块根的诱导营养液及诱导培养方法 (Induction nutrient solution and induction culture method for rehmannia root tuber ) 是由 薛涛 薛建平 段永波 戈圆圆 刘鑫 刘嫚 江珊珊 姜雪云 施江 熊雨婕 于 2020-05-07 设计创作，主要内容包括：本发明涉及组织培养技术领域,具体涉及一种地黄块根的诱导营养液及诱导培养方法。每L所述营养液中包括如下组分：黄腐酸12-13mg、硝酸铵115-125mg、磷酐22-26mg、氧化钾155-160mg、氧化钙80-83mg、硫酸镁23-26mg、EDTA-Mn0.25-0.26mg、EDTA-Fe0.25-0.26mg、EDTA-Zn0.033-0.036mg、EDTA-Cu 0.005mg、钼酸铵0.005mg,硼酸0.005mg；以水定容。本发明还提供了利用该营养液诱导培养地黄块根的方法,二者配合能够促进地黄块根的诱导,提高地黄块根的移栽萌发率和移栽萌发率,突破解决了试管地黄块根易愈伤化,后期难以萌发的技术难点。(The invention relates to the technical field of tissue culture, in particular to an induction nutrient solution and an induction culture method for a rehmannia root tuber. Each L of the nutrient solution comprises the following components: 12-13mg of fulvic acid, 125mg of ammonium nitrate 115-160 mg of phosphoric anhydride, 22-26mg of potassium oxide 155-160mg of calcium oxide, 80-83mg of magnesium sulfate, 23-26mg of EDTA-Mn0.25-0.26mg of EDTA-Fe0.25-0.26mg of EDTA-Zn0.033-0.036mg of EDTA-Cu0.005mg, 0.005mg of ammonium molybdate and 0.005mg of boric acid; the volume is determined by water. The invention also provides a method for inducing and culturing the rehmannia root tuber by using the nutrient solution, and the two are matched to promote the induction of the rehmannia root tuber, improve the transplanting germination rate and the transplanting germination rate of the rehmannia root tuber, and break through the technical difficulties that the rehmannia root tuber in a test tube is easy to callus and difficult to germinate in the later period.)

1. The nutrient solution for inducing the rehmannia root tuber is characterized in that each L of the nutrient solution comprises the following components: 12-13mg of fulvic acid, 125mg of ammonium nitrate 115-26 mg, 22-26mg of phosphoric anhydride, 160mg of potassium oxide 155-83 mg, 80-83mg of calcium oxide, 23-26mg of magnesium sulfate, 0.25-0.26mg of EDTA-Mn0.25-0.26mg, 0.25-0.26mg of EDTA-Fe0.033-0.036 mg, 0.005mg of EDTA-Cu0.005mg, 0.005mg of ammonium molybdate and 0.005mg of boric acid;

the volume is determined by water.

2. The nutrient solution for inducing root tuber of rehmannia according to claim 1, wherein the pH value of the nutrient solution is in the range of 5.5 to 6.0.

3. The method for inducing and culturing the rehmannia root tuber by using the nutrient solution as claimed in claim 2, which comprises the following steps:

detoxifying and rapidly propagating rehmannia test-tube plantlets, then performing rooting and strong seedling culture on the obtained detoxified rehmannia seedlings, transferring the rehmannia seedlings after rooting to an aeroponic system for culture, wherein the culture illumination intensity is 12000lux, the culture temperature is 25 ℃, nutrient solution is regularly sprayed to roots of the rehmannia seedlings during culture, calculation is started from transferring to the aeroponic system, the early spraying period is set to be 20 min/time, 15s is sprayed each time, the spraying rate is 2mL/s, the spraying period is adjusted to be 40 min/time after 7-8d of culture is performed, new roots are generated, 15s is sprayed each time, and after 80-90d of culture, the generated root tubers are cut and transferred to soil for germination.

Technical Field

The invention relates to the technical field of tissue culture, in particular to an induction nutrient solution and an induction culture method for a rehmannia root tuber.

Background

Rehmannia is a medicinal herbaceous plant of the Scrophulariaceae family, and root tuber of rehmannia is used as a medicine, is one of the bulk medicinal materials commonly used in China, and has the effects of resisting tumor, resisting aging, reducing blood sugar, enhancing immunity and the like. Rehmannia is widely distributed in China, mainly field cultivation, and is mostly distributed in Shanxi, Henan, Anhui, Sichuan and other places, wherein Henan jiao serves as a genuine producing area with rehmannia. For a long time, the rhizome asexual propagation method is mostly adopted for rehmannia, which causes virus diseases to flood, causes severe degeneration of rehmannia varieties, and affects the yield and quality of the rehmannia varieties. Aiming at plant virus diseases, no safe and effective drug treatment way exists at present, and the detoxification culture mainly depends on the detoxification culture of plant stem tips to further culture detoxification seedlings, but the detoxification seedlings have the defects of inconvenient transportation, low transplanting survival rate and the like, and the popularization and the application of the detoxification seedlings are influenced to a certain degree.

Based on the defect of popularization of detoxified test-tube plantlets, research reports the induction of test-tube radix rehmanniae tuberous roots, and the application prospect of producing artificial seeds by the test-tube radix rehmanniae tuberous roots is expected, but the induced test-tube radix rehmanniae tuberous roots are easy to callus and difficult to germinate in the later period, so that the popularization and application in the later period are limited. Therefore, developing a convenient and easy-to-popularize rehmannia glutinosa seedling cultivation method becomes a scientific problem to be solved urgently in rehmannia glutinosa planting production.

Disclosure of Invention

In order to solve the technical problems, the invention provides an induction nutrient solution and an induction culture method for a rehmannia root tuber.

In order to achieve the purpose, the invention adopts the following technical scheme:

an induction nutrient solution for radix rehmanniae tuberous roots, wherein each L of the nutrient solution comprises the following components: 12-13mg of fulvic acid, 125mg of ammonium nitrate 115-22 mg of phosphoric anhydride, 160mg of potassium oxide 155-160mg of calcium oxide, 80-83mg of calcium oxide, 23-26mg of magnesium sulfate, 0.25-0.26mg of EDTA-Mn0.25-0.26mg of EDTA-Fe0.25-0.26mg, 0.033-0.036mg of EDTA-Cu0.005mg of ammonium molybdate and 0.005mg of boric acid;

the volume is determined by water.

Furthermore, the pH value of the nutrient solution is within the range of 5.5-6.0.

The invention also provides a method for inducing and culturing the rehmannia root tuber by using the nutrient solution, which comprises the following steps:

detoxifying and rapidly propagating rehmannia test-tube plantlets, then performing rooting and seedling strengthening culture on the obtained detoxified rehmannia seedlings, transferring the rehmannia seedlings after rooting to an aeroponic culture system for culture, wherein the culture illumination intensity is 12000lux, the culture temperature is 25 ℃, nutrient solution is sprayed regularly during the culture period, the early spraying period is set to be 20 min/time, spraying is performed for 15s each time, after new roots are generated after culture for 7-8d, the spraying period is adjusted to be 40 min/time, spraying is performed for 15s each time, after culture for 80-90d, the generated root tubers are cut and transferred to soil for germination.

Compared with the prior art, the invention has the following technical effects:

the nutrient solution takes fulvic acid and boric acid as main effect factors to play an inducing role, and is matched with other components to provide nutrient elements, wherein the element C is derived from fulvic acid, the element N is derived from ammonium nitrate and ammonium molybdate, the element P is derived from phosphoric anhydride, the element K is derived from potassium oxide, the element Ca is derived from calcium oxide, the element Mg is derived from magnesium sulfate, the element Mn is derived from EDTA-Mn, the element Fe is derived from EDTA-Fe, the element Zn is derived from EDTA-Zn, the element Cu is derived from EDTA-Cu, the element Mo is derived from ammonium molybdate, and the element B is derived from boric acid.

The nutrient solution is matched with the induction method, so that the induction of the radix rehmanniae tuberous root can be promoted, the transplanting germination rate and the transplanting germination rate of the radix rehmanniae tuberous root are improved, and the technical difficulties that the radix rehmanniae tuberous root in a test tube is easy to heal and difficult to germinate in the later period are solved.

Drawings

FIG. 1 is a rooting culture of detoxified rehmannia seedlings.

FIG. 2A shows the atomization culture of virus-free rehmannia seedlings, and B shows the root tuber induction.

FIG. 3A is the root tuber after induction of detoxified rehmannia seedlings, and B is the germination test result.

Detailed Description

The invention is described in detail below with reference to the figures and the specific embodiments, but the invention should not be construed as being limited thereto. The technical means used in the following examples are conventional means well known to those skilled in the art, and materials, reagents and the like used in the following examples can be commercially available unless otherwise specified.