阅读说明：本技术 植物酶活测定用前处理装置 (Pretreatment device for measuring plant enzyme activity ) 是由 戴军 汪玉娟 叶梦娟 宋程 宋向文 卫培培 于 2020-03-25 设计创作，主要内容包括：本发明公开了植物酶活测定用前处理装置,包括具有一容纳空间的壳体,所述壳体上端开有与所述容纳空间连通的进料口,所述壳体的容纳空间内还还包括从上至下依次设置的粉碎腔、暂存腔、安装腔和离心腔,所述暂存腔与所述安装腔之间固定设有第一分隔板,所述安装腔与所述分离腔之间固定设有第二分隔板；所述粉碎腔内固定设有对称设置的第一粉碎辊和第二粉碎辊,所述第一粉碎辊和第二粉碎辊上均设有粉碎刀和刮料板；所述安装腔用于安装定量分配机构,用于向离心腔中的离心管中定量添加溶液。本发明实现了自动化处理,提高了酶活测定前处理的效率,且植物酶的提取效果好。(The invention discloses a pretreatment device for measuring the activity of a plant enzyme, which comprises a shell with a containing space, wherein a feed inlet communicated with the containing space is formed in the upper end of the shell, the containing space of the shell also comprises a crushing cavity, a temporary storage cavity, an installation cavity and a centrifugal cavity which are sequentially arranged from top to bottom, a first partition plate is fixedly arranged between the temporary storage cavity and the installation cavity, and a second partition plate is fixedly arranged between the installation cavity and the separation cavity; a first crushing roller and a second crushing roller which are symmetrically arranged are fixedly arranged in the crushing cavity, and a crushing knife and a scraping plate are arranged on each of the first crushing roller and the second crushing roller; the installation cavity is used for installing a quantitative distribution mechanism and is used for quantitatively adding solution into a centrifugal tube in the centrifugal cavity. The invention realizes automatic treatment, improves the efficiency of enzyme activity determination pretreatment, and has good extraction effect of the plant enzyme.)

1. The pretreatment device for measuring the activity of the plant enzyme is characterized by comprising a shell with a containing space, wherein a feed inlet communicated with the containing space is formed in the upper end of the shell, the containing space of the shell also comprises a crushing cavity, a temporary storage cavity, a mounting cavity and a centrifugal cavity which are sequentially arranged from top to bottom, a first partition plate is fixedly arranged between the temporary storage cavity and the mounting cavity, and a second partition plate is fixedly arranged between the mounting cavity and the separation cavity;

a first crushing roller and a second crushing roller which are symmetrically arranged are fixedly arranged in the crushing cavity, crushing knives and scraping plates are arranged on the first crushing roller and the second crushing roller respectively, and the crushing cavity is used for crushing plant materials;

the temporary storage cavity is used for collecting the filtered solution in the crushing cavity;

the mounting cavity is used for mounting a quantitative distribution mechanism, the liquid inlet end of the quantitative distribution mechanism is communicated with the temporary storage cavity, and the liquid outlet end of the quantitative distribution mechanism is positioned in the centrifugal cavity and is used for quantitatively adding a solution into a centrifugal tube in the centrifugal cavity;

the centrifugal mechanism is arranged in the centrifugal cavity, a plurality of centrifugal tubes are inserted into the centrifugal mechanism, and the centrifugal tubes can receive the solution conveyed by the quantitative distribution mechanism.

2. The pretreatment device for measuring plant enzyme activity according to claim 1, wherein a filter plate is disposed between the pulverization chamber and the temporary storage chamber, the filter plate comprises a support block located in the middle and filter screens fixedly connected with the support block and located on both sides of the support block, the filter plate is detachably connected with the inner wall of the housing, a sliding plate is slidably disposed on the upper end of the filter plate, a sliding groove is disposed on the lower end of the middle of the sliding plate, a protruding strip extends upwards from the upper end of the support frame, the protruding strip is inserted into the sliding groove and slidably connected with the sliding groove, one end of the sliding plate penetrates through the side wall of the housing and is fixedly connected with a transmission mechanism located outside the housing, and the sliding plate is slidably connected with the side wall of the housing.

3. The pretreatment device for measuring plant enzyme activity according to claim 2, wherein squeezing plates are hinged to both sides of the inner wall of the crushing chamber, the squeezing plates are rotatably connected with the inner wall of the crushing chamber, and a torsion spring is sleeved on a connecting portion between the squeezing plates and the crushing chamber.

4. The pretreatment device for measuring plant enzyme activity according to claim 3, wherein the pressing plate, the central axis of the first pulverizing roller, and the central axis of the second pulverizing roller are positioned on the same horizontal line.

5. The pretreatment device for measuring plant enzyme activity according to claim 2, wherein the filter screens located on both sides of the support frame are arc-shaped, and the centers of the arcs are located on the axes of the first crushing roller and the second crushing roller, respectively.

6. The pretreatment device for measuring plant enzyme activity according to claim 1, wherein the quantitative distribution mechanism comprises a distribution chamber, one end of the distribution chamber is communicated with a liquid inlet pipe and a liquid outlet pipe, one end of the liquid inlet pipe, which is far away from the quantitative distribution mechanism, penetrates through the first distribution plate and is communicated with the temporary storage chamber, one end of the liquid outlet pipe, which is far away from the quantitative distribution mechanism, penetrates through the second distribution plate and is located in the centrifugal chamber, a first check valve is inserted into the liquid inlet pipe, a second check valve is inserted into the liquid outlet pipe, the distribution chamber further comprises a piston in interference fit with the inner wall of the distribution chamber, one end of the piston, which is far away from the liquid inlet pipe, is fixedly provided with a pull rod, one end of the pull rod, which is far away from the piston, penetrates through the side wall of one end of the quantitative distribution mechanism and is fixedly connected with a, the driving component is used for driving the pull rod to reciprocate along the horizontal direction.

7. The pretreatment device for measuring plant enzyme activity according to claim 5, wherein the drive assembly comprises a drive block, a transmission cavity is formed in the drive block, racks are fixedly arranged at the upper end and the lower end of the transmission cavity, an incomplete gear is further included in the transmission cavity, the incomplete gear is meshed with the racks, and the incomplete gear is driven to rotate by a drive motor.

8. The pretreatment device for measuring plant enzyme activity according to claim 1, wherein the centrifugation mechanism comprises a rotating disc and a motor fixed at the lower end of the rotating disc and used for driving the rotating disc to rotate, a placing disc is arranged at the upper end of the rotating disc, an electric telescopic rod is fixedly arranged between the placing disc and the rotating disc and used for driving the placing disc to move up and down relative to the rotating disc, a plurality of placing grooves which are uniformly distributed are formed in the upper end of the placing disc, the circle centers of the plurality of placing grooves are located on the same circumference, and the placing grooves are used for placing centrifuge tubes.

9. The pretreatment device for measuring plant enzyme activity according to claim 7, wherein a limit post is further fixedly provided at an upper end of the rotary disk, a limit groove matched with the limit post is provided on the placing disk, and a height of the limit post is smaller than a maximum range of the electric telescopic rod and larger than a minimum range of the electric telescopic rod.

10. The pretreatment device for measuring the activity of the plant enzyme is characterized by comprising an upper shell and a lower shell which are provided with a containing space, wherein the lower end of the upper shell is detachably connected with the upper end of the lower shell;

the upper end of the upper shell is provided with a feed inlet communicated with the containing space, and the containing space of the upper shell also comprises a crushing cavity and a temporary storage cavity which are sequentially arranged from top to bottom;

a first crushing roller and a second crushing roller which are symmetrically arranged are fixedly arranged in the crushing cavity, crushing knives and scraping plates are arranged on the first crushing roller and the second crushing roller respectively, and the crushing cavity is used for crushing plant materials;

the temporary storage cavity is used for collecting the filtered solution in the crushing cavity, and a liquid discharge port is formed in the lower end of the temporary storage cavity;

the lower shell is sequentially provided with an installation cavity and a centrifugal cavity from top to bottom, a first partition plate is fixedly arranged on one side, away from the centrifugal cavity, of the installation cavity, and a second partition plate is fixedly arranged between the installation cavity and the separation cavity;

the mounting cavity is used for mounting a quantitative distribution mechanism, the liquid inlet end of the quantitative distribution mechanism is communicated with the liquid outlet at the lower end of the temporary storage cavity, and the liquid outlet end of the quantitative distribution mechanism is positioned in the centrifugal cavity and used for quantitatively adding a solution into a centrifugal tube in the centrifugal cavity;

the centrifugal mechanism is arranged in the centrifugal cavity, a plurality of centrifugal tubes are inserted into the centrifugal mechanism, and the centrifugal tubes can receive the solution conveyed by the quantitative distribution mechanism.

Technical Field

The invention relates to the technical field of enzyme activity determination, in particular to a pretreatment device for determining plant enzyme activity.

Background

At present, the pretreatment of plant enzyme activity detection mainly comprises the steps of low-temperature treatment, tissue disruption, centrifugation and the like, and specifically comprises the steps of adding a cleaned plant material into a mortar, adding liquid nitrogen into the mortar, carrying out manual grinding, adding a proper amount of buffer solution after the grinding is finished, dissolving, filtering by using filter paper, centrifuging by using a centrifuge, and taking supernatant. The method not only wastes time and labor, but also has the problem of poor extraction effect.

Disclosure of Invention

Based on the technical problems in the background art, the invention provides the pretreatment device for the plant enzyme activity determination, which realizes automatic treatment, improves the efficiency of pretreatment for the enzyme activity determination, and has good extraction effect of plant enzyme.

The pretreatment device for measuring the activity of the plant enzyme comprises a shell with a containing space, wherein a feed inlet communicated with the containing space is formed in the upper end of the shell, a crushing cavity, a temporary storage cavity, an installation cavity and a centrifugal cavity are sequentially arranged in the containing space of the shell from top to bottom, a first partition plate is fixedly arranged between the temporary storage cavity and the installation cavity, and a second partition plate is fixedly arranged between the installation cavity and the separation cavity;

a first crushing roller and a second crushing roller which are symmetrically arranged are fixedly arranged in the crushing cavity, crushing knives and scraping plates are arranged on the first crushing roller and the second crushing roller respectively, and the crushing cavity is used for crushing plant materials;

the temporary storage cavity is used for collecting the filtered solution in the crushing cavity;

the mounting cavity is used for mounting a quantitative distribution mechanism, the liquid inlet end of the quantitative distribution mechanism is communicated with the temporary storage cavity, and the liquid outlet end of the quantitative distribution mechanism is positioned in the centrifugal cavity and is used for quantitatively adding a solution into a centrifugal tube in the centrifugal cavity;

the centrifugal mechanism is arranged in the centrifugal cavity, a plurality of centrifugal tubes are inserted into the centrifugal mechanism, and the centrifugal tubes can receive the solution conveyed by the quantitative distribution mechanism.

Preferably, smash the chamber with be equipped with the filter between the chamber of keeping in, the filter including be located the middle part the supporting shoe and with supporting shoe fixed connection just is located the filter screen of supporting shoe both sides, the filter with the connection can be dismantled to the inner wall of casing, the filter upper end still slides and is equipped with the sliding plate, sliding plate middle part lower extreme is equipped with the spout, support frame upper end upwards extends there is the sand grip, the sand grip peg graft in the spout and with spout sliding connection, sliding plate one end is run through the lateral wall of casing and with be located the drive mechanism fixed connection in the casing outside, just the sliding plate with the lateral wall sliding connection of casing.

Preferably, the inner wall both sides in crushing chamber still articulate there is the stripper plate, the stripper plate with the inner wall in crushing chamber rotates to be connected, just the stripper plate with the connecting portion cover in crushing chamber is equipped with the torsional spring.

Preferably, the pressing plate, the central axis of the first crushing roller and the central axis of the second crushing roller are located on the same horizontal line.

Preferably, the filter screens that are located the support frame both sides all are circular-arc, and the centre of a circle of circular arc is located the axis that is located first crushing roller and second crushing roller respectively.

Preferably, the quantitative distribution mechanism comprises a distribution cavity, one end of the distribution cavity is communicated with a liquid inlet pipe and a liquid outlet pipe, one end of the liquid inlet pipe, which is far away from the quantitative distribution mechanism, penetrates through the first distribution plate and is communicated with the temporary storage cavity, one end of the liquid outlet pipe, which is far away from the quantitative distribution mechanism, penetrates through the second distribution plate and is positioned in the centrifugal cavity, a first one-way valve is inserted on the liquid inlet pipe, a second one-way valve is inserted on the liquid outlet pipe, a piston in interference fit with the inner wall of the distribution cavity is further arranged in the distribution cavity, the piston is kept away from the fixed pull rod that is equipped with of one end of feed liquor pipe, the one end that the pull rod was kept away from the piston runs through quantitative distribution mechanism's one end lateral wall and with be located the drive assembly fixed connection in the quantitative distribution mechanism outside, drive assembly is used for driving the pull rod along the reciprocating displacement of horizontal direction.

Preferably, the drive assembly includes the drive block, the inside of drive block is opened there is the transmission chamber, both ends are fixed and are equipped with the rack about the transmission chamber, still include incomplete gear in the transmission chamber, incomplete gear with rack toothing, incomplete gear drives its rotation through a driving motor.

Preferably, centrifugal mechanism includes the rolling disc and fixes at the rolling disc lower extreme and be used for driving rolling disc pivoted motor, the rolling disc upper end is equipped with places the dish, place the dish with fixed electric telescopic handle that is equipped with between the rolling disc, electric telescopic handle is used for driving and places the relative rolling disc of dish and produce displacement from top to bottom, place the dish upper end and open the standing groove that has a plurality of evenly distributed, a plurality of the centre of a circle of standing groove is located same circumference, the standing groove is used for placing the centrifuging tube.

Preferably, the upper end of the rotating disc is further fixedly provided with a limiting column, the placing disc is provided with a limiting groove matched with the limiting column, and the height of the limiting column is smaller than the maximum range of the electric telescopic rod and larger than the minimum range of the electric telescopic rod.

The pretreatment device for measuring the activity of the plant enzyme comprises an upper shell and a lower shell, wherein the upper shell is provided with a containing space;

the upper end of the upper shell is provided with a feed inlet communicated with the containing space, and the containing space of the upper shell also comprises a crushing cavity and a temporary storage cavity which are sequentially arranged from top to bottom;

a first crushing roller and a second crushing roller which are symmetrically arranged are fixedly arranged in the crushing cavity, crushing knives and scraping plates are arranged on the first crushing roller and the second crushing roller respectively, and the crushing cavity is used for crushing plant materials;

the temporary storage cavity is used for collecting the filtered solution in the crushing cavity, and a liquid discharge port is formed in the lower end of the temporary storage cavity;

the lower shell is sequentially provided with an installation cavity and a centrifugal cavity from top to bottom, a first partition plate is fixedly arranged on one side, away from the centrifugal cavity, of the installation cavity, and a second partition plate is fixedly arranged between the installation cavity and the separation cavity;

the mounting cavity is used for mounting a quantitative distribution mechanism, the liquid inlet end of the quantitative distribution mechanism is communicated with the liquid outlet at the lower end of the temporary storage cavity, and the liquid outlet end of the quantitative distribution mechanism is positioned in the centrifugal cavity and used for quantitatively adding a solution into a centrifugal tube in the centrifugal cavity;

the centrifugal mechanism is arranged in the centrifugal cavity, a plurality of centrifugal tubes are inserted into the centrifugal mechanism, and the centrifugal tubes can receive the solution conveyed by the quantitative distribution mechanism.

Compared with the prior art, the invention has the beneficial technical effects that:

(1) the pretreatment device for measuring the activity of the plant enzyme can realize the automatic treatment of crushing, filtering and centrifuging of plant materials, improves the working efficiency and has good extraction effect.

(2) The quantitative distribution mechanism is arranged in the installation cavity, so that automatic quantitative addition in the centrifugal tube is realized, and the structure is simple and convenient.

(3) The centrifugal mechanism and the quantitative distribution mechanism are matched for use, so that the volume of the solution added into the centrifugal tube can be ensured to be the same, the problem of unbalanced mass distribution of the rotating disc during centrifugation is avoided, the centrifugal tube can be subjected to centrifugal treatment, and the clarity of the solution is further improved.

Drawings

FIG. 1 is a schematic view showing the structure of a pretreatment apparatus for measuring plant enzyme activity according to the present invention;

fig. 2 is a schematic structural diagram of a quantitative dispensing mechanism according to the present invention;

FIG. 3 is a schematic structural diagram of a tray according to the present invention;

FIG. 4 is a partial enlarged view A-A of the present invention;

FIG. 5 is a schematic view showing the structure of a pretreatment device for measuring plant enzyme activity according to another embodiment of the present invention.

In the figure: 1-shell, 2-sliding plate, 3-extrusion plate, 4-first crushing roller, 5-feed inlet, 6-crushing cutter, 7-second crushing roller, 8-crushing chamber, 9-filter plate, 10-temporary storage chamber, 11-installation chamber, 12-rotating disc, 13-electric telescopic rod, 14-motor, 15-limit column, 16-placing disc, 17-scraping plate, 18-limit groove, 19-placing groove, 20-convex strip, 21-sliding groove, 22-supporting block, 23-filter screen, 24-liquid inlet pipe, 25-first one-way valve, 26-second one-way valve, 27-liquid outlet pipe, 28-piston, 29-pull rod, 30-pressure relief hole, 31-transmission chamber, 32-incomplete gear, 33-a driving block, 34-a rack, 35-a distribution cavity, 36-a quantitative distribution mechanism, 37-a first partition plate, 38-a centrifugal cavity, 39-a second partition plate, 40-an upper shell and 41-a lower shell.

Detailed Description

The present invention will be further illustrated with reference to the following specific examples.