阅读说明：本技术 脱氧雪腐镰刀菌烯醇及其衍生物的消减方法 (Method for reducing deoxynivalenol and derivatives thereof ) 是由 方海琴 章程 刘爱东 梁春来 徐伟东 刘飒娜 刘玉洁 邓陶陶 支媛 杨辉 于 2020-03-20 设计创作，主要内容包括：本发明涉及食品科学领域,具体而言,涉及一种脱氧雪腐镰刀菌烯醇及其衍生物的消减方法,该方法利用高压气体放电产生低温等离子体对脱氧雪腐镰刀菌烯醇和/或其衍生物进行消减。该方法具有消减率高、无需高温、保留食物营养与风味、无残留等诸多优势。(The invention relates to the field of food science, in particular to a method for reducing deoxynivalenol and derivatives thereof. The method has the advantages of high reduction rate, no need of high temperature, retained nutrition and flavor of food, no residue, etc.)

1. The method for reducing the deoxynivalenol and the derivatives thereof is characterized in that the deoxynivalenol and/or the derivatives thereof are reduced by using low-temperature plasma generated by high-pressure gas discharge.

2. The method of claim 1, wherein the derivative is an acetylated derivative of deoxynivalenol.

3. The method according to claim 2, wherein the derivative is selected from the group consisting of 3-acetyl deoxynivalenol and 15-acetyl deoxynivalenol.

4. The method according to any one of claims 1 to 3, wherein the atmosphere of the high-pressure gas discharge is any one or more of air, an oxygen-containing gas, nitrogen, and a rare gas.

5. A method according to any one of claims 1 to 3, wherein the power output for the high pressure gas discharge is a sinusoidal voltage.

6. The method of claim 5, wherein the power supply has a voltage amplitude of 3 to 8kV and a frequency of 10 to 50kHz when performing the subtraction.

7. The method of claim 6, wherein the time of the subtraction is ≧ 1 min.

8. The method of claim 7, wherein the time for the abatement is from 4min to 6 min.

9. Use of a method according to any one of claims 1 to 8 for the abatement of deoxynivalenol and/or a derivative thereof in a grain, a grain processing by-product or a grain product.

10. Use according to claim 9, characterized in that the grains are selected from:

any one or combination of corn, wheat, barley, oat, rye, highland barley, rice, millet, sorghum, coix seed, teff, tassel, quinoa, buckwheat and quinoa.

Technical Field

The invention relates to the field of food science, in particular to a reduction method of deoxynivalenol and derivatives thereof.

Background

Deoxynivalenol (DON) is a deoxyderivative of nivalenol, is a water-soluble trichothecene mycotoxin produced by secondary metabolism of fusarium, has a chemical name of 3,7, 15-trihydroxy-12, 13-epoxy trichothecene-9-ene-8-one and a molecular formula of C₁₅H₂₀O₆And the molecular weight is 296.3. Deoxynivalenol, also known as vomitoxin, widely exists in corn, wheat, barley, oat, rye, rice and other grain crops in the natural world, and enters a food chain through grains and products thereof, such as bread and the like, so that the pollution condition is serious. 3-acetyl deoxynivalenol (3-ADON) and 15-acetyl deoxynivalenol (15-ADON) are the most common DON derivatives, and have been detected in cereals and products thereof in recent years.

DON causes a series of toxic reactions in humans and animals, such as dizziness, vomiting, diarrhea, unresponsiveness, decreased appetite, central nervous system disorders, and the like. The world health organization food additives Joint Experts Committee (JECFA) conference 56 defined the PMTDI (tentative maximum daily tolerance) of DON as 1. mu.g/kgbw/d. After this, JECFA modified it again to a limit of 1. mu.g/kgbw/d for the DON, 3-ADON, 15-ADON compound group at 72 th conference in 2010. In 2017, 10 and 27, after a carcinogen list published by international cancer research institution of world health organization is preliminarily collated, the deoxynivalenol is in the three carcinogen lists.

The existing DON reduction and control methods mainly comprise physical, chemical and biological methods, however, the methods have the defects of energy consumption, easy introduction of secondary pollution, limited large-scale application and the like, and no effective method is found at home and abroad for the reduction of 3-ADON and 15-ADON. Therefore, it is important to develop efficient, safe and green DON reduction technology research.

Disclosure of Invention

The invention aims to solve the problems of complex operation on Deoxynivalenol (DON), generation of additional pollutants, low reduction efficiency, poor applicability and the like in the prior art, break through the dilemma that the DON derivatives (3-ADON, 15-ADON) are not effectively attenuated at home and abroad at present, and simultaneously, the DON and the derivatives thereof treated by the method have low cytotoxicity and can effectively ensure the national food safety.

In order to achieve the above purpose of the present invention, the following technical solutions are adopted:

the invention relates to a method for reducing deoxynivalenol and derivatives thereof, which utilizes low-temperature plasma generated by high-pressure gas discharge to reduce the deoxynivalenol and/or the derivatives thereof.

Optionally, the derivative is an acetylated derivative of deoxynivalenol.

Optionally, the derivative is selected from 3-acetyl deoxynivalenol (3-ADON) and 15-acetyl deoxynivalenol (15-ADON).

Optionally, the atmosphere of the high-voltage gas discharge is any one or more of air, oxygen-containing gas, nitrogen and rare gas.

Optionally, the output of the power supply used for the high-voltage gas discharge is a sinusoidal voltage.

Optionally, when the power supply is reduced, the voltage amplitude of the power supply is 3-8 kV, and the frequency is 10-50 kHz.

Optionally, the reduction time is more than or equal to 1 min.

Optionally, the reduction time is 4min to 6 min.

The invention also relates to the use of a method as described above for the reduction of deoxynivalenol and/or derivatives thereof in cereals or cereal products.

Optionally, the grain is selected from:

any one or combination of corn, wheat, barley, oat, rye, highland barley, rice, millet, sorghum, coix seed, teff, tassel, quinoa (kaniwa), buckwheat and quinoa.

Compared with the prior art, the method provided by the invention has the advantages that various components such as ultraviolet rays, high-energy charged particles, active substances (molecules, excited state atoms, metastable state atoms and free radicals) and the like generated in the low-temperature plasma generating process can comprehensively act on DON and derivatives thereof, so that the effect of reducing toxins is achieved. Compared with the traditional attenuation technology, the method has the advantages of good effect, no need of high temperature, food nutrition and flavor conservation, no residue and the like.

The invention selects low-temperature plasma treatment as a core technology, utilizes high-pressure gas discharge to generate low-temperature plasma, develops experimental research for reducing mycotoxin, degrades DON concentration and reduces DON toxicity. A brand-new path and scientific basis are provided for constructing an efficient, safe and green DON reduction technology.

The method has a DON reduction rate of more than 50%, and has good effect on 3-ADON and 15-ADON which are difficult to reduce by other methods. Wherein, the reduction rate of 3-ADON can reach about 24-26 percent, and the reduction rate of 15-ADON can reach about 20 percent.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a comparison of the growth state of DON 120 μ g/ml cells after plasma treatment in one embodiment of the present invention;

FIG. 2 is a graph showing the cytotoxic dose response of DON 120 μ g/ml after plasma treatment in accordance with one embodiment of the present invention;

FIG. 3 is a comparison of the growth state of 3-ADON cells treated with plasma at different concentrations in one embodiment of the present invention;

FIG. 4 is a graph of the cytotoxic dose-response of 3-ADON after plasma treatment in accordance with one embodiment of the present invention;

FIG. 5 is a comparison of the growth state of cells treated with plasma at different concentrations of 15-ADON in one embodiment of the present invention;

FIG. 6 is a graph of the cytotoxic dose-response of 15-ADON after plasma treatment in accordance with one embodiment of the present invention.

Detailed Description

Reference will now be made in detail to embodiments of the invention, one or more examples of which are described below. Each example is provided by way of explanation, not limitation, of the invention. In fact, it will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the scope or spirit of the invention. For instance, features illustrated or described as part of one embodiment, can be used on another embodiment to yield a still further embodiment.

It is therefore intended that the present invention cover the modifications and variations of this invention provided they come within the scope of the appended claims and their equivalents. Other objects, features and aspects of the present invention are disclosed in or are apparent from the following detailed description. It is to be understood by one of ordinary skill in the art that the present discussion is a description of exemplary embodiments only, and is not intended as limiting the broader aspects of the present invention.

The invention relates to a method for reducing deoxynivalenol and derivatives thereof, which utilizes low-temperature plasma generated by high-pressure gas discharge to reduce the deoxynivalenol and/or the derivatives thereof.

The existing state of the substance generally presents a solid, liquid and gaseous conversion process along with the rise of the temperature, and the basic forms of the three substances are called three states of the substance. In the case of a substance in a gaseous state, if the temperature rises to several thousand degrees centigrade or higher, the molecules of the substance are accelerated by thermal motion, and the gas molecules are ionized by the violent collision with each other, and then the substance becomes a mixture of free electrons and positive ions, and this state of the substance is called a fourth state of the substance, i.e., a plasma state.

According to the invention, a high-voltage power supply is adopted to connect a plasma electrode for micro-discharge, and various components such as ultraviolet rays, high-energy charged particles, active substances (molecules, excited atoms, metastable atoms and free radicals) and the like generated in the process of generating low-temperature plasma can comprehensively act on DON and derivatives thereof, so that the effect of reducing toxins is achieved. Compared with the traditional DON reduction technology, the low-temperature plasma technology has the obvious advantages of low cost, no waste, no pollution and the like; and has the advantages of high safety, no drug residue, short treatment process and time, no environmental pollution and the like. For 3-ADON and 15-ADON which are difficult to effectively reduce by physical, chemical and biological digestion methods adopted in the prior art, the invention can also achieve a more ideal reduction rate.

In conclusion, the method provided by the invention has a good application prospect.

In some embodiments, the derivative is an acetylated derivative of deoxynivalenol.

In some embodiments, the derivative is selected from the group consisting of 3-acetyl deoxynivalenol and 15-acetyl deoxynivalenol.

The gas used in the high-pressure gas discharge plasma-generating treatment process may be, for example, an oxygen-containing gas such as O₂、H₂O、NO₂Nitrogen and air, or inert gases (e.g., helium, neon, argon, krypton, xenon); or a mixture of the above gases. Preference is given to oxygen-containing gases, in particular O₂And air. The gas pressure may be atmospheric pressure or lower.

In some embodiments, the reducing is performed at ambient temperature, e.g., 10 ℃ to 40 ℃, or 18 ℃,20 ℃,25 ℃.

In some embodiments, the output of the power source used for the high-voltage gas discharge is a high-frequency alternating voltage, preferably a sinusoidal voltage, and a pulsed voltage and a pulsed direct voltage can also be used.

In some embodiments, the voltage amplitude of the power supply is 3 to 8kV and the frequency is 10 to 50kHz when the subtraction is performed.

In some embodiments, the voltage amplitude of the power supply can be selected to be 2, 5, 6 or 7kV and the frequency can be selected to be 15, 20, 25, 30, 35, 40 or 45kHz, preferably 25-35 kHz, when the subtraction is performed.

In some embodiments, the discharge electrode adopts a flat plate type dielectric barrier discharge structure, the upper electrode and the lower electrode adopt circular aluminum flat plate electrodes, the diameter of the electrode is 60 mm-80 mm, the edge radius of the electrode is 8 mm-12 mm, and the effective area of the electrode during discharge is 18cm²～21cm²(about 19.6 cm)²)。

In some embodiments, a container for holding the object to be treated is placed on the lower electrode, and the container can be a glass plate.

In some embodiments, the time of the subtraction is ≧ 1min, e.g., 2min, 3min, 4min, 5min, 6min, 7min, 8min, 9min, 10min, 11min, 12min, 13min, 14min, 15min or more.

Although the low-temperature plasma technology does not need harsh operating conditions such as high temperature, high pressure, chemical reagent addition and the like, the low-temperature plasma technology has higher power consumption because of being generated under the high-pressure discharge condition, so that the cost for treating the wastewater by the technology is relatively higher; the invention finds that when DON and the derivative thereof are reduced, when the reduction time is about 5min, the reduction effect is not obviously strengthened by increasing the treatment time. Therefore, in order to improve the energy utilization rate, reduce the energy consumption and reduce the treatment cost, in a more preferred embodiment, the reduction time is 4min to 6min, and most preferably about 5 min.

According to a further aspect of the invention, the invention relates to the use of a method as described above for the abatement of deoxynivalenol and/or a derivative thereof in cereals, cereal processing by-products or cereal products.

In some embodiments, the grain is selected from:

any one or combination of corn, wheat, barley, oat, rye, highland barley, rice, millet, sorghum, coix seed, teff, tassel, quinoa (kaniwa), buckwheat and quinoa.

The grain may be ground granular or powdered.

Before the reduction, the grains may be further processed by impurity removal, such as vibrating screen, stone remover, self-circulation air separator, etc.

In some embodiments, the cereal product is a feed.

Embodiments of the present invention will be described in detail with reference to examples.