阅读说明：本技术 一种草鱼超级免疫方法 (Super immunization method for grass carps ) 是由 谢金燕 于 2018-08-25 设计创作，主要内容包括：本发明属于水产养殖技术领域,具体涉及一种草鱼超级免疫方法。本发明使用免疫刺激剂聚氨基酸钠,壳聚糖树突细胞进行刺激,使树突细胞发育和增值,捕获机体内致病细菌,提呈给免疫T细胞,使免疫T细胞对机体内存在的多种致病细菌产生免疫效果,对防治出血、赤皮、烂鳃、肠炎等草鱼疾病有良好的预防作用,使鱼体能产生更长效、更光谱的细菌性疾病的预防效果,对部分的细菌产生终生免疫效果。超级免疫技术操作简便,无需人工注射操作,对鱼的机械损伤大幅降低,减小养殖过程的病害防治压力,可满足绿色、环保、高效、安全等养殖要求。(The invention belongs to the technical field of aquaculture, and particularly relates to a grass carp super-immune method. The invention uses the immunostimulant sodium polyamino acid and chitosan dendritic cells for stimulation, so that the dendritic cells develop and proliferate, pathogenic bacteria in an organism are captured, and the pathogenic bacteria are presented to immune T cells, so that the immune T cells have an immune effect on various pathogenic bacteria in the organism, and have a good prevention effect on grass carp diseases such as hemorrhage, red skin, gill rot, enteritis and the like, so that the fish body can generate a longer-acting and more-spectral prevention effect on bacterial diseases, and a lifelong immune effect on part of bacteria. The super-immune technology is simple and convenient to operate, manual injection operation is not needed, mechanical damage to the fish is greatly reduced, the disease control pressure in the culture process is reduced, and the culture requirements of environmental protection, high efficiency, safety and the like can be met.)

1. A grass carp super-immunization method is characterized by comprising the following steps: A. cleaning the body surface of grass carp fry by using fish body surface treatment liquid, wherein each 1L of the surface treatment liquid contains 0.05-2mL of 38-40 wt% formaldehyde solution, 0.001-0.0035g of benzalkonium chloride, 0.04-0.08g of glycolic acid and 10-20mL of glycerol;

B. adding immunostimulant into clean water; the concentration is 20-50mml/kg according to the weight of grass carp, and 20-40ml/m according to the volume of water³；

C. Soaking grass carp fry in clean water with immunostimulant for 20-160min for 1 time.

2. The grass carp hyperimmunization method according to claim 1, wherein the grass carp hyperimmunization method comprises the following steps:

A. cleaning the body surface of grass carp fry by using fish body surface treatment liquid, wherein each 1L of the surface treatment liquid contains 0.1-2mL of 38-40 wt% formaldehyde solution, 0.002-0.0035g of benzalkonium chloride, 0.05-0.07g of glycolic acid and 10-20mL of glycerol; soaking grass carp fry in the fish body surface treatment solution for 1-3 min;

B. washing: transferring to clear water tank for washing for 1-2 min;

C. adding immunostimulant into clean water; the concentration is 30-40mml/kg according to the weight of grass carp, and 30ml/m according to the volume of water³；

D. Soaking grass carp fry in clean water with immunostimulant for 60-120min for 1 time.

3. The method for immunizing fish according to claim 1 or 2, wherein in step A, the whole course is oxygenated and the concentration of immunostimulant is 30mml/kg based on the weight of the fish.

4. The method for hyperimmunizing grass carp according to any one of claims 1-3, wherein the immunostimulant is selected from the group consisting of sodium polyamino acid and chitosan.

Technical Field

The invention belongs to the technical field of aquaculture, and particularly relates to a grass carp super-immune method.

Background

Grass carp is a common food for residents in China, is an important source for protein intake of human bodies, and people put forward higher requirements on nutrition and taste of grass carp along with improvement of living standard, and grass carp has fishy smell during eating, so that the taste is influenced, the quality of grass carp is reduced, and a large-scale fishery breeding mode is modernized.

The Nobel medical awarded leading Canadian scientist Lalfstein man discovers and reveals the action of dendritic cells in an immune system, and the grass carp super-immune technology is characterized in that an immunostimulant is used for stimulating the dendritic cells to enable the dendritic cells to develop and proliferate, capturing pathogenic bacteria in a body and presenting the pathogenic bacteria to immune T cells, so that the immune T cells have an immune effect on various pathogenic bacteria in the body, and the grass carp super-immune technology has a good prevention effect on grass carp diseases such as bleeding, red skin, gill rot, enteritis and the like, so that the fish body can generate a longer-acting and more-spectral prevention effect on bacterial diseases, and can generate a life-long immune effect on part of bacteria. The super-immune technology is simple and convenient to operate, manual injection operation is not needed, mechanical damage to the fish is greatly reduced, the disease control pressure in the culture process is reduced, and the culture requirements of environmental protection, high efficiency, safety and the like can be met.

Disclosure of Invention

The invention aims to provide a grass carp super-immunization method aiming at the existing problems.

The invention is realized by the following technical scheme:

a grass carp super-immunization method is characterized by comprising the following steps:

A. cleaning the body surface of grass carp fry by using fish body surface treatment liquid, wherein each 1L of the surface treatment liquid contains 0.05-2mL of 38-40 wt% formaldehyde solution, 0.001-0.0035g of benzalkonium chloride, 0.04-0.08g of glycolic acid and 10-20mL of glycerol;

B. adding immunostimulant into clean water; the concentration is 20-50mml/kg according to the weight of grass carp, and 20-40ml/m according to the volume of water³；

C. Soaking grass carp fry in clean water with immunostimulant for 20-160min for 1 time.

Further, the grass carp hyperimmunization method comprises the following steps:

A. cleaning the body surface of grass carp fry by using fish body surface treatment liquid, wherein each 1L of the surface treatment liquid contains 0.1-2mL of 38-40 wt% formaldehyde solution, 0.002-0.0035g of benzalkonium chloride, 0.05-0.07g of glycolic acid and 10-20mL of glycerol; soaking grass carp fry in the fish body surface treatment solution for 1-3 min;

B. washing: transferring to clear water tank for washing for 1-2 min;

C. adding immunostimulant into clean water; the concentration is 30-40mml/kg according to the weight of grass carp, and 30ml/m according to the volume of water³；

D. Soaking grass carp fry in clean water with immunostimulant for 60-120min for 1 time.

Further, in the step A, the whole process is oxygenated, and the concentration of the immunostimulant is 30mml/kg based on the weight of the fish.

Further, the immunostimulant is sodium polyamino acid and chitosan.

The beneficial technical effects are as follows:

compared with the prior art, the invention has the following advantages: the invention provides an environment-friendly and sustainable-development grass carp breeding method, the bred grass carp is accelerated in growth speed, soft and delicious in meat quality, rich in nutrition and not easy to get ill, and the yield of the grass carp is obviously increased. The invention adopts a super-immune technology, namely, an immunostimulant is used for stimulating dendritic cells, so that the dendritic cells develop and proliferate, pathogenic bacteria in a body are captured, and the pathogenic bacteria are presented to immune T cells, so that the immune T cells have an immune effect on various pathogenic bacteria in the body, and have a good prevention effect on grass carp diseases such as hemorrhage, red skin, gill rot, enteritis and the like, so that the fish body can generate a longer-acting and more-spectral prevention effect on bacterial diseases, and a lifelong immune effect on part of bacteria is generated. The super-immune technology is simple and convenient to operate, manual injection operation is not needed, mechanical damage to the fish is greatly reduced, the disease control pressure in the culture process is reduced, and the culture requirements of environmental protection, high efficiency, safety and the like can be met.

Description of the drawings:

FIG. 1: without using super immunization technique, the melanocytes are in round dot shape

FIG. 2: the melanocytes are dendritic using hyperimmunization technology

Detailed Description

The present invention will be further described with reference to specific examples, which are by no means intended to limit the scope of the invention

With any limitations evident.

Comparative example 1: without using superimmunization techniques

Soaking the grass carp fry in clean water for two hours.

And (3) detection reference of immune effect: after soaking, the macroscopic microscope checks whether the fish scale black cells of the fish appear to extend in a circular shape, and the result is shown in figure 1.