阅读说明：本技术 用于微流控芯片的液流导向阀及微流控芯片 (Liquid flow guide valve for micro-fluidic chip and micro-fluidic chip ) 是由 闵小平 葛胜祥 苏晓崧 张师音 张东旭 赵巍 郭清顺 张军 夏宁邵 于 2018-08-22 设计创作，主要内容包括：本发明涉及一种用于微流控芯片的液流导向阀及微流控芯片。其中,用于微流控芯片的液流导向阀包括：转动件,其内设有导流通道,且设有与所述导流通道对应的第一导流孔和第二导流孔；所述第一导流孔用于将所述导流通道与所述转动件外部的第一液流通道连通；所述第二导流孔用于将所述导流通道与所述转动件外部的第二液流通道连通；以及定位件,用于将所述转动件限位于芯片本体,且允许所述转动件相对于所述芯片本体转动,以使所述第一导流孔可选择性地与所述芯片本体内的不同的第一液流通道连通,所述第二导流孔始终与所述芯片本体内的同一第二液流通道连通。本发明通过简单的旋转转动体即可实现液流的导向功能,操作方便,能够提高工作效率。(The invention relates to a liquid flow guide valve for a micro-fluidic chip and the micro-fluidic chip. Wherein, a liquid flow guide valve for a microfluidic chip comprises: the rotating piece is internally provided with a flow guide channel and a first flow guide hole and a second flow guide hole which correspond to the flow guide channel; the first flow guide hole is used for communicating the flow guide channel with a first liquid flow channel outside the rotating piece; the second diversion hole is used for communicating the diversion channel with a second liquid flow channel outside the rotating piece; and the positioning piece is used for limiting the rotating piece on the chip body and allowing the rotating piece to rotate relative to the chip body, so that the first flow guide holes can be selectively communicated with different first liquid flow channels in the chip body, and the second flow guide holes are always communicated with the same second liquid flow channel in the chip body. The invention can realize the liquid flow guiding function through a simple rotary rotating body, is convenient to operate and can improve the working efficiency.)

1. A fluid flow directing valve for a microfluidic chip, comprising:

the rotating part (1) is internally provided with a flow guide channel (11) and is provided with a first flow guide hole (111) and a second flow guide hole (112) corresponding to the flow guide channel (11); the first flow guide hole (111) is used for communicating the flow guide channel (11) with a first liquid flow channel (31) outside the rotating piece (1); the second diversion hole (112) is used for communicating the diversion channel (11) with a second liquid channel (32) outside the rotating piece (1); and

the positioning piece (2) is used for limiting the rotating piece (1) to the chip body (3) and allowing the rotating piece (1) to rotate relative to the chip body (3), so that the first flow guide hole (111) can be selectively communicated with different first liquid flow channels (31) in the chip body (3), and the second flow guide hole (112) is always communicated with the same second liquid flow channel (32) in the chip body (3).

2. The liquid flow directing valve for microfluidic chips according to claim 1, wherein the rotating member (1) comprises:

a main body (12) provided with a groove (121) for forming a flow guide channel (11); and

the back plate (13) is arranged on the main body (12) and matched with the groove (121) to form the flow guide channel (11), and the first flow guide hole (111) and the second flow guide hole (112) are arranged on the back plate (13).

3. The liquid flow directing valve for a microfluidic chip according to claim 1,

a through hole (21) is formed in the positioning piece (2), the through hole (21) comprises a first through hole part and a second through hole part, and the size of the first through hole part is smaller than that of the second through hole part;

the rotor (1) comprises a first portion (14) and a second portion (15); the first portion (14) having a size smaller than the first through-hole portion, the first portion (14) passing through the second through-hole portion and the first through-hole portion in this order; the second portion (15) is larger than the first through hole portion and smaller than the second through hole portion, and the second portion (15) is located in the second through hole portion.

4. The liquid flow guide valve for microfluidic chip according to claim 1, wherein the rotating member (1) is circular, and the second guiding hole (112) is disposed at the center of the rotating member (1).

5. The liquid flow directing valve for microfluidic chips according to claim 4, wherein the flow guiding channel (11) extends from the second flow guiding hole (112) to a radial direction of the rotation member (1).

6. The fluid flow directing valve for a microfluidic chip according to claim 1, wherein the second flow guiding hole (112) is located at the center of the rotation member (1).

7. The fluid flow directing valve for a microfluidic chip according to claim 6, wherein the first flow guiding hole (111) is located below a portion of the positioning member (2) for pressing the rotation member (1) toward the chip body (3).

8. The liquid flow directing valve for microfluidic chips according to claim 1, wherein a spacer (4) is provided between the rotating member (1) and the positioning member (2).

9. The liquid flow directing valve for microfluidic chip according to claim 1, wherein the rotating member (1) is provided with a first light-transmitting hole (17), and the first light-transmitting hole (17) is located at one side of the first flow-guiding hole (111).

10. The liquid flow directing valve for microfluidic chips according to claim 1, wherein the rotating member (1) is provided with an instrument engagement portion (16), the instrument engagement portion (16) being adapted to engage with an instrument for rotating the rotating member (1) to facilitate rotation of the rotating member (1).

11. The liquid flow guide valve for microfluidic chip according to claim 1, wherein the rotating member (1) is provided with a plurality of sliding blocks (18) at intervals along the circumference, and the sliding blocks (18) slide in contact with the positioning members (2) to reduce friction between the rotating member (1) and the positioning members (2) during rotation.

12. A microfluidic chip, comprising a chip body (3) and a liquid flow guide valve for a microfluidic chip according to any one of claims 1 to 11, wherein the positioning member (2) is fixed to the chip body (3).

13. The microfluidic chip according to claim 12, wherein at least two first flow channels (31) are disposed in the chip body (3), each of the first flow channels (31) is configured with a first flow hole (311) and a second flow hole (312), the first flow hole (311) is used for communicating the first flow channel (31) with the flow guide channel (11), and the second flow hole (312) is used for communicating the first flow channel (31) with a reagent storage bin, a waste liquid bin or a secondary reagent reaction bin.

14. The microfluidic chip according to claim 13, wherein the first flow guiding hole (111) of the flow guiding channel (11) is selectively aligned with the first flow hole (311) of one of the first flow channels (31) during rotation of the rotating member (1).

15. The microfluidic chip according to claim 13, wherein the first flow hole (311) and the second flow hole (312) are both provided on the same surface of the chip body (3).

16. The microfluidic chip according to claim 13, wherein a second fluid channel (32) is disposed in the chip body (3), the chip body (3) is provided with a third fluid hole (321) and a fourth fluid hole (322), the third fluid hole (321) is used for communicating the second fluid channel (32) with the flow guide channel (11), and the fourth fluid hole (322) is used for communicating the second fluid channel (32) with a primary reagent reaction chamber.

17. The microfluidic chip of claim 16, wherein the third flow hole (321) is aligned with the second flow guiding hole (112).

18. The microfluidic chip according to claim 16, wherein the first flow hole (311) of each of the first flow channels (31) is disposed around the third flow hole (321) of the second flow channel (32), and each of the first flow holes (311) is equidistant from the third flow hole (321).

19. The microfluidic chip according to claim 16, wherein the third flow hole (321) and the fourth flow hole (322) are both disposed on the same surface of the chip body (3).

20. The microfluidic chip according to claim 13, wherein one side of the first flow guiding hole (111) of the flow guiding channel (11) is provided with a first light transmitting hole (17), one side of the first flow hole (311) of each of the first flow channels (31) is provided with a second light transmitting hole, and in a state where the first flow guiding hole (111) is aligned with one of the first flow holes (311), the first light transmitting hole (17) is aligned with the second light transmitting hole on one side of the first flow hole (311) and is light-transmitting, and can be detected by a light sensor, so as to monitor and calibrate the position of the rotating member (1).

21. The microfluidic chip according to claim 12, wherein the positioning element (2) is fixed to the chip body (3) by gluing, snap-fit connection or screw-locking.

22. The microfluidic chip according to claim 12, comprising a positioning ring (5), wherein the positioning ring (5) is integrally formed with the chip body (3), and the positioning member (2) is in threaded connection with the positioning ring (5).

23. The microfluidic chip according to claim 12, wherein the center of the flow directing valve and the valve area of the chip body (3) are provided with positioning structures (6) that cooperate with each other to position the flow directing valve mounted on the chip body (3).

Technical Field

The invention relates to the field of microfluidic detection, in particular to a liquid flow guide valve for a microfluidic chip and the microfluidic chip.

Background

Due to its high integration and strong automation, the microfluidic chip technology is increasingly applied to point-of-care testing (POCT) in clinical testing projects. However, if a certain detection performance is required for a general complete biochemical reaction, especially for nucleic acid and immunoassay, a complete reaction process needs to be reproduced on a chip. On the other hand, the complete reaction flow is formed by the participation of various liquids including the sample, and the whole reaction process involves a large amount of liquid directional and sequential flow control. Therefore, for the microfluidic chip for nucleic acid and immune reaction, a valve assembly for controlling the flow of the sample, the reagent and the waste liquid according to the requirement is required to be introduced. The valve assembly should meet the following requirements: 1) the processing is simple, and large-scale industrial mass production can be realized; 2) easy integration and small difficulty in combination with a chip; 3) the accurate release and flow guide of different reagents can be realized; 4) the operation is simple and the realization is easy.

Disclosure of Invention

One of the objectives of the present invention is to provide a liquid flow guiding valve for a microfluidic chip and a microfluidic chip with convenient operation.

Some embodiments of the present invention provide a liquid flow directing valve for a microfluidic chip, comprising: the rotating piece is internally provided with a flow guide channel and a first flow guide hole and a second flow guide hole which correspond to the flow guide channel; the first flow guide hole is used for communicating the flow guide channel with a first liquid flow channel outside the rotating piece; the second diversion hole is used for communicating the diversion channel with a second liquid flow channel outside the rotating piece; and the positioning piece is used for limiting the rotating piece on the chip body and allowing the rotating piece to rotate relative to the chip body, so that the first flow guide holes can be selectively communicated with different first liquid flow channels in the chip body, and the second flow guide holes are always communicated with the same second liquid flow channel in the chip body.

Optionally, the rotating member comprises: the main body is provided with a groove for forming a flow guide channel; and the back plate is arranged on the main body and matched with the groove to form the flow guide channel, and the first flow guide hole and the second flow guide hole are arranged on the back plate.

Optionally, a through hole is arranged in the positioning element, the through hole includes a first through hole portion and a second through hole portion, and the size of the first through hole portion is smaller than that of the second through hole portion; the rotating member includes a first portion and a second portion; the size of the first part is smaller than that of the first through hole part, and the first part sequentially penetrates through the second through hole part and the first through hole part; the second portion is larger than the first through hole portion and smaller than the second through hole portion, and the second portion is located in the second through hole portion.

Optionally, the rotating member is circular, and the second diversion hole is formed in the center of the rotating member.

Optionally, the flow guide channel extends from the second flow guide hole to a radial direction of the rotating member.

Optionally, the second pilot hole is located at a center of the rotating member.

Optionally, the first diversion hole is located below a portion of the positioning element, where the positioning element is used to press the rotating element towards the chip body.

Optionally, a gasket is disposed between the rotating member and the positioning member.

Optionally, the rotating member is provided with a first light hole, and the first light hole is located on one side of the first diversion hole.

Optionally, the rotating member is provided with an instrument mating portion for mating with an instrument rotating the rotating member to facilitate rotation of the rotating member.

Optionally, a plurality of sliding blocks are arranged at intervals in the circumferential direction of the rotating part, and the sliding blocks slide in contact with the positioning parts to reduce friction between the rotating part and the positioning parts in the rotating process.

Some embodiments of the present invention provide a microfluidic chip, which includes a chip body and the above-mentioned liquid flow guiding valve for a microfluidic chip, wherein the positioning element is fixed on the chip body.

Optionally, at least two first liquid flow channels are arranged in the chip body, each first liquid flow channel is provided with a first liquid flow hole and a second liquid flow hole, the first liquid flow hole is used for communicating the first liquid flow channel with the flow guide channel, and the second liquid flow hole is used for communicating the first liquid flow channel with a reagent storage bin, a waste liquid bin or a secondary reagent reaction bin.

Optionally, during rotation of the rotating member, the first flow guiding hole of the flow guiding channel can be selectively aligned with the first flow hole of one of the first flow channels.

Optionally, the first flow hole and the second flow hole are both disposed on the same surface of the chip body.

Optionally, a second liquid flow channel is arranged in the chip body, the chip body is provided with a third liquid flow hole and a fourth liquid flow hole, the third liquid flow hole is used for communicating the second liquid flow channel with the flow guide channel, and the fourth liquid flow hole is used for communicating the second liquid flow channel with the primary reagent reaction bin.

Optionally, the third flow aperture is aligned with the second diversion aperture.

Optionally, the first flow hole of each first flow channel is arranged around the third flow hole of the second flow channel, and each first flow hole is equidistant from the third flow hole.

Optionally, the third flow hole and the fourth flow hole are both disposed on the same surface of the chip body.

Optionally, a first light hole is formed in one side of a first flow guiding hole of the flow guiding channel, a second light hole is formed in one side of a first liquid flow hole of each first liquid flow channel, and when the first flow guiding hole is aligned with one of the first liquid flow holes, the first light hole is aligned with the second light hole in one side of the first liquid flow hole and is transparent, and the first light hole and the second light hole can be detected by an optical sensor to monitor and calibrate the position of the rotating member.

Optionally, the fixing mode of the positioning element and the chip body is gluing, buckling connection or thread locking.

Optionally, the microfluidic chip comprises a positioning ring, the positioning ring and the chip body are integrally formed, and the positioning piece is in threaded connection with the positioning ring.

Optionally, a positioning structure that is matched with the valve area of the chip body is arranged at the center of the liquid flow guide valve, so as to position the liquid flow guide valve installed on the chip body.

Based on the technical scheme, the invention at least has the following beneficial effects:

in some embodiments, the liquid flow guiding valve for the microfluidic chip includes a rotating member and a positioning member, wherein the positioning member is used for limiting the rotating member to the chip body and allowing the rotating member to rotate relative to the chip body, so that the first flow guiding holes can be selectively communicated with different first liquid flow channels in the chip body, and the second flow guiding holes are always communicated with the same second liquid flow channel in the chip body. The guide function of liquid flow can be realized through a simple rotary rotating body, the operation is convenient, and the working efficiency can be improved.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention without limiting the invention. In the drawings:

fig. 1(a) is a schematic top view of a rotor according to some embodiments of the present invention;

FIG. 1(b) is a schematic bottom view of a rotatable member according to some embodiments of the present invention;

fig. 2 is a schematic diagram of a liquid flow directing valve for a microfluidic chip according to some embodiments of the present invention assembled with a chip body;

fig. 3 is an exploded view of a liquid flow directing valve and a chip body for a microfluidic chip according to some embodiments of the present invention;

fig. 4(a) is a schematic top view of a liquid flow directing valve for a microfluidic chip according to some embodiments of the present invention in a closed state after being assembled with a chip body;

fig. 4(b) is a schematic top view of an assembled liquid flow directing valve and chip body for a microfluidic chip according to some embodiments of the present invention;

fig. 5(a) is a schematic cross-sectional view of a closed state of a liquid flow directing valve for a microfluidic chip according to some embodiments of the present invention after being assembled with a chip body;

fig. 5(b) is a schematic cross-sectional view of an assembled open state of a liquid flow guide valve for a microfluidic chip and a chip body according to some embodiments of the present invention;

fig. 6 is a schematic diagram of a rotor rotatably connecting different liquid flow channels after a liquid flow guide valve for a microfluidic chip according to some embodiments of the present invention is assembled with a chip body;

fig. 7 is an exploded view of a liquid flow directing valve for a microfluidic chip according to some embodiments of the present invention assembled with a chip body after being provided with a gasket;

fig. 8 is a schematic cross-sectional view of a liquid flow directing valve for a microfluidic chip according to some embodiments of the present invention assembled with a chip body after being provided with a gasket;

fig. 9 is a schematic cross-sectional view of a positioning structure disposed between a liquid flow guiding valve and a chip body for a microfluidic chip according to some embodiments of the present invention;

fig. 10 is an exploded view of a liquid flow directing valve for a microfluidic chip and a chip body provided with a positioning ring according to some embodiments of the present invention;

fig. 11 is a schematic cross-sectional view of an assembled liquid flow directing valve for a microfluidic chip and a chip body provided with a positioning ring according to some embodiments of the present invention;

fig. 12(a) is a schematic diagram of a closed state of a liquid flow guiding valve and a chip body provided with light-transmitting holes for a microfluidic chip according to some embodiments of the present invention;

fig. 12(b) is a schematic diagram illustrating an open state of a liquid flow guiding valve and a chip body for a microfluidic chip according to some embodiments of the present invention after a light hole is formed;

fig. 13 is a schematic diagram of a slider disposed on a rotor of a liquid flow directing valve for a microfluidic chip according to some embodiments of the present invention.

The reference numbers in the drawings:

1-a rotating member; 11-a flow guide channel; 111-a first flow guiding hole; 112-second flow guiding holes; 12-a body; 121-grooves; 122-positioning holes; 13-a back plate; 14-first site; 15-second site; 16-an instrument engagement portion; 17-a first light-transmitting hole; 18-a slide block;

2-a positioning element; 21-a through hole;

3-a chip body; 31-a first flow channel; 311-first flow orifice; 312 — a second flow orifice; 32-a second flow channel; 321-a third flow aperture; 322-fourth flowbore;

4-a gasket;

5-a positioning ring;

6-a positioning structure;

a-off state; b-the communication state of the first liquid flow channel; c-the communication state of the second first liquid flow channel; d-the communication state of the third first liquid flow channel; e-the connected state of the fourth first liquid flow channel; f-the communication state of the fifth first liquid flow channel.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments. It is to be understood that the described embodiments are merely a few embodiments of the invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without any inventive step, are within the scope of the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate orientations or positional relationships based on those shown in the drawings, and are used merely for convenience in describing the present invention and for simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be taken as limiting the scope of the present invention.

The liquid flow guide valve for the micro-fluidic chip has the functions of pipeline communication and liquid flow guide.

In some embodiments, as shown in fig. 1(a), 1(b), a liquid flow directing valve for a microfluidic chip comprises a rotor 1. A flow guide channel 11 (shown in fig. 5 (b)) is provided in the rotating member 1. The rotating member 1 is further provided with a first guiding hole 111 and a second guiding hole 112 (as shown in fig. 3) corresponding to the guiding channel 11.

The first guide hole 111 is used to communicate the guide passage 11 with the first flow passage 31 outside the rotation member 1. Namely: a first end of the first guide hole 111 communicates with the guide passage 11, and a second end of the first guide hole 111 is adapted to communicate with the first flow passage 31 outside the rotation member 1.

The second guide hole 112 is used to communicate the guide passage 11 with the second flow passage 32 outside the rotation member 1. Namely: the first end of the second guiding hole 112 is communicated with the guiding passage 11, and the second end of the second guiding hole 112 is used for communicating with the second fluid passage 32 outside the rotating member 1.

In some embodiments, as shown in fig. 2, the liquid guiding valve for a microfluidic chip includes a positioning member 2, the positioning member 2 is used for limiting the rotating member 1 to the chip body 3 and allowing the rotating member 1 to rotate relative to the chip body 3, so that the first guiding holes 111 can be selectively communicated with different first liquid flow channels 31 in the chip body 3, and the second guiding holes 112 are always communicated with the same second liquid flow channel 32 in the chip body 3.

In the above embodiment, the positioning element 2 is fixed relative to the chip body 3, and the rotating element 1 can rotate freely between the positioning element 2 and the chip body 3.

In some embodiments, the rotating member 1 is combined with the positioning member 2, and the liquid flow guiding function can be realized by simply rotating the rotating member 1, so that the operation is convenient, and the working efficiency can be improved. The liquid flow guide valve is matched with the chip body 3, so that a plurality of reagent storage bins, a waste liquid bin and a secondary reagent reaction bin of the micro-fluidic chip can be selectively communicated with the primary reagent reaction bin, and the micro-fluidic chip is easy to control.

In some embodiments, as shown in fig. 13, a plurality of sliding blocks 18 are disposed at intervals in the circumferential direction of the rotating member 1, and the sliding blocks 18 slide in contact with the positioning members 2 to reduce friction between the rotating member 1 and the positioning members 2 during rotation.

Alternatively, two or more sliders 18 may be provided in the circumferential direction of the rotating member 1.

Optionally, the rotating member 1 is circular, the positioning member 2 is a circular ring, the diameter of the rotating member 1 may be slightly smaller than the inner diameter of the positioning member 2, and three or more protrusions, i.e., sliding blocks 18, extend outward in the circumferential direction of the rotating member 1. The distance from the center of the rotating member 1 to the edge of the slider 18 is equal to the inner radius of the positioning member 2. With this arrangement, the frictional resistance caused by the friction between the outer edge of the rotating member 1 and the inner edge of the positioning member 2 when the rotating member 1 rotates can be significantly reduced.

In some embodiments, as shown in fig. 3, the rotating member 1 includes a main body 12, and the main body 12 is provided with a groove 121 (shown in fig. 1 (b)) for forming the flow guide channel 11.

In some embodiments, as shown in fig. 3, the rotating member 1 includes a back plate 13, and the back plate 13 is disposed on the main body 12. Optionally, the back plate 13 is bonded to the body 12 in a snug manner. The back plate 13 and the groove 121 cooperate to form a flow guide channel 11, and the first flow guide hole 111 and the second flow guide hole 112 are disposed on the back plate 13.

In some embodiments, the back plate 13 may be a gasket. Optionally, the back plate 13 is a circular sheet rubber structure. The material of the back plate 13 may be an organic polymer synthetic material with low friction, wear resistance and corrosion resistance, such as polytetrafluoroethylene.

In some embodiments, the main body 12 and the back plate 13 are bonded by gluing, the back plate 13 is provided with positioning holes 122 (as shown in fig. 1(b) and fig. 3), and the positioning of the back plate 13 is completed by the cooperation of the positioning pins and the positioning holes 122 to ensure the smoothness of the liquid path.

In some embodiments, as shown in fig. 3, a through hole 21 is formed in the positioning member 2, and the through hole 21 includes a first through hole portion and a second through hole portion, and the size of the first through hole portion is smaller than that of the second through hole portion.

As shown in fig. 1(a), the rotating member 1 includes a first portion 14 and a second portion 15; the size of the first portion 14 is smaller than the size of the first through hole portion. The first portion 14 passes through the second through-hole portion and the first through-hole portion in this order; the second portion 15 has a size larger than the first through hole portion and smaller than the second through hole portion, the second portion 15 being located within the second through hole portion.

As shown in fig. 1(a), the rotation member 1 is provided with an instrument engagement portion 16, and the instrument engagement portion 16 is used to engage with an instrument for rotating the rotation member 1 to facilitate rotation of the rotation member 1.

In some embodiments, the instrument engagement portion 16 is configured as a female or male configuration to engage a male or female configuration on an instrument used to rotate the rotational member 1. Alternatively, the concave structure is in the shape of a straight line, a cross star, a star, etc., which can achieve a secure fit.

As shown in fig. 7 and 8, a gasket 4 may be provided between the rotating member 1 and the positioning member 2 in order to ensure smooth rotation of the rotating member 1 and sealing of the fluid flow guide valve in various orientations.

Optionally, the gasket 4 is a circular ring and is made of materials such as polytetrafluoroethylene, the polytetrafluoroethylene has a self-lubricating property, the low friction force and the deformation property of the polytetrafluoroethylene can greatly reduce the friction coefficient between the rotating part 1 and the positioning part 2, and the sealing property between the rotating part 1 and the positioning part 2 is improved.

In some embodiments, the rotating member 1 is circular, and the second guiding hole 112 is disposed at the center of the rotating member 1. Optionally, the positioning element 2 associated therewith is also circular.

In some embodiments, the guide passage 11 extends from the second guide hole 112 toward the radial direction of the rotation member 1.

In some embodiments, the rotation member 1 is not limited to a circular shape, and the second pilot hole 112 is located at the center of the rotation member 1.

In some embodiments, the first guide hole 111 is located below a portion of the positioning member 2 for pressing the rotation member 1 toward the chip body 3, so as to improve the sealing between the fluid flow guide valve and the chip body 3.

In some embodiments, the rotating member 1 is provided with a first light hole 17, and the first light hole 17 is located at one side of the first guiding hole 111.

The microfluidic chip system in the related prior art lacks a liquid flow guide valve with high efficiency, low cost and simple structure, and the liquid flow guide valve disclosed by the invention can simply realize the respective connection and the rapid switching of one reaction bin and a plurality of reagent bins. Meanwhile, the liquid flow guide valve is simple in working principle, can be integrated with any type of chip body through simple pipeline design, and is high in universality. On the other hand, the liquid flow guide valve disclosed by the invention is simple in structure, can be quickly formed through die sinking, can be produced in batches, and greatly reduces the production and application cost of chips.

Some embodiments provide a microfluidic chip, which includes a chip body 3 and a liquid flow guide valve for the microfluidic chip in any of the above embodiments, wherein a positioning member 2 of the liquid flow guide valve is fixed to the chip body 3. The liquid flow guide valve which is designed independently is integrated with the chip body 3 of the micro-fluidic chip, so that the functions of liquid flow conduction and flow direction guide of the liquid flow guide valve are exerted. The liquid flow guide valve can realize the connection and disconnection of liquid flow through simple rotation.

In some embodiments, at least two first flow channels 31 are disposed in the chip body 3, each first flow channel 31 is provided with a first flow hole 311 and a second flow hole 312, the first flow hole 311 is used for communicating the first flow channel 31 with the flow guide channel 11, and the second flow hole 312 is used for communicating the first flow channel 31 with a reagent storage bin, a waste liquid bin or a secondary reagent reaction bin.

In some embodiments, the first guiding hole 111 of the guiding passage 11 can be selectively aligned with the first flow hole 311 of one of the first flow passages 31 during the rotation of the rotating member 1. The switching among the first liquid flow channels 31 is completed through the rotation of the rotating piece 1 of the liquid flow guide valve, so that the communication between the primary reagent reaction bin and the plurality of reagent storage bins, or the waste liquid bin or the secondary reagent reaction bin can be realized, the operation is convenient, and the working efficiency is improved. And the diversion channel 11 and the main-level reagent reaction bin can be repeatedly washed by subsequent reagents, so that the residue of the pre-reagents can be greatly reduced, and the accuracy and reliability of the reaction result are ensured.

As shown in fig. 3, the first and second flow holes 311 and 312 are provided on the same surface of the chip body 3.

As shown in fig. 5(a) and 5(b), a second flow channel 32 is provided in the chip body 3, the chip body 3 is provided with a third flow hole 321 and a fourth flow hole 322, the third flow hole 321 is used for communicating the second flow channel 32 with the flow guide channel 11, and the fourth flow hole 322 is used for communicating the second flow channel 32 with the primary reagent reaction chamber.

It should be noted that, the liquid flow guide valve in the present disclosure may guide the reagent in the reagent storage bin to the primary reagent reaction bin, may also guide the reagent in the primary reagent reaction bin to the secondary reagent reaction bin, and may also guide the reagent in the primary reagent reaction bin to the waste liquid bin, but is not limited to the above-mentioned drainage connection relationship.

In some embodiments, third flow aperture 321 communicates with second flow aperture 112. Further, the third flow hole 321 is aligned with the second guide hole 112.

As shown in fig. 4(a) and 5(a), in the closed state of the flow guide valve, the first guide hole 111 of the guide passage 11 is not communicated with the first flow hole 311 of any of the first flow passages 31, and the second guide hole 112 of the guide passage 11 is always communicated with the third flow hole 321 of the second flow passage 32.

As shown in fig. 4(b) and 5(b), in the open state of the flow guide valve, the first guide hole 111 of the guide passage 11 is in communication with the first flow hole 311 of one first flow passage 31 of the plurality of first flow passages 31, and the second guide hole 112 of the guide passage 11 is always in communication with the third flow hole 321 of the second flow passage 32.

In some embodiments, as shown in fig. 6, five first flow channels 31 are provided in the chip body 3, and the first flow channels 31 can be communicated by rotating the flow guide valve to the corresponding first flow channels 31. The method can be realized specifically as follows: closing the state A; a communicating state B of the first liquid flow channel; a communicating state C of a second first liquid flow channel, a communicating state D of a third first liquid flow channel; a communicating state E of the fourth first liquid flow channel; and a fifth first fluid flow path communication state F.

In some embodiments, the first flow hole 311 of each first flow channel 31 is disposed around the third flow hole 321 of the second flow channel 32, and each first flow hole 311 is equidistant from the third flow hole 321.

In some embodiments, the third and fourth flow holes 321 and 322 are provided on the same surface of the chip body 3.

In some embodiments, the fixing manner of the positioning element 2 and the chip body 3 is gluing. Optionally, the positioning element 2 is bonded to the surface of the chip body 3 by ultraviolet glue, AB glue or super glue.

As shown in fig. 12(a) and 12(b), a first light hole 17 is formed at one side of the first flow hole 111 of the flow guide channel 11, a second light hole is formed at one side of the first flow hole 311 of each first flow channel 31, and in a state where the first flow hole 111 is aligned with one of the first flow holes 311, the first light hole 17 is aligned with the second light hole at one side of the first flow hole 311 and transmits light, which can be detected by an optical sensor, so as to monitor and calibrate the position of the valve.

As shown in fig. 12(a), in the closed state of the liquid flow guide valve, the first guide hole 111 is not aligned with any of the first liquid flow holes 311, and the first light-transmitting hole 17 is not aligned with any of the second light-transmitting holes, and does not transmit light.

As shown in fig. 12(b), when the liquid flow guiding valve is in the open state, the first guiding hole 111 is aligned with one of the first liquid flow holes 311, and the first light transmission hole 17 is aligned with the second light transmission hole on one side of the first liquid flow hole 311, so that light transmission from the bottom to the upper part of the whole chip can be realized.

In some embodiments, only these light-transmitting holes may be exposed by providing the flow guide valve and the chip body 3 with a housing and a light-shielding cover. Then, a series of photoelectric sensors or photoelectric positioning devices are arranged in the instrument, so that when the liquid flow guide valve rotates, the light holes in the liquid flow guide valve are aligned or deviated with the light holes in the chip body 3, and the opening position of the liquid flow guide valve is monitored or calibrated in real time.

The opening position of the liquid flow guide valve is positioned by photoelectric induction, the requirement on an instrument motor is lower, and the positioning precision is higher than that of the rotary positioning of the valve through a servo motor.

In some embodiments, the fixing manner of the positioning member 2 and the chip body 3 is a snap connection.

Optionally, a set of buckles is arranged on the chip body 3, and after the liquid flow guide valve is pressed in, the liquid flow guide valve is clamped on the chip body 3 through elastic deformation of the buckles.

In some embodiments, the fixing manner of the positioning member 2 and the chip body 3 is a screw locking manner.

As shown in fig. 10 and 11, the microfluidic chip includes a positioning ring 5, the positioning ring 5 is integrally formed with the chip body 3, and the positioning member 2 is in threaded connection with the positioning ring 5.

The tightness degree of the liquid flow guide valve is quantitatively installed through the number of threads, the whole installation stability is superior to that of gluing, an automatic assembly line is formed by the whole installation stability, and automatic assembly of chips in the later period is facilitated.

Optionally, on the chip body 3, a circular ring-shaped protrusion not higher than the rotating member 1 is provided as the positioning ring 5, with the third liquid flow hole 321 as a center and the diameter of the rotating member 1 as an inner diameter.

As shown in FIG. 9, in order to facilitate the positioning of the liquid flow guide valve in cooperation with the chip body 3, a positioning structure 6 is provided at the center of the liquid flow guide valve and the valve area of the chip body 3 for mounting the liquid flow guide valve, wherein the positioning structure is engaged with each other to position the liquid flow guide valve mounted on the chip body 3.

In some embodiments, the positioning structure 6 may be a male-female mating structure.

Alternatively, the center of the flow guide valve is provided with a downwardly convex curved surface, and correspondingly, the chip body 3 is provided with a downwardly concave curved surface. The positioning of the liquid flow guide valve and the chip body 3 is realized through the matching of the downward convex curved surface and the downward concave curved surface. The liquid flow guide valve and the chip body 3 are matched with each other in a high-precision matching mode, so that the precision of subsequent assembly, the rate of finished products of chips and the production efficiency of the chips are greatly improved. Simultaneously, pressure and deformation between little structure more help the sealed of central main pipeline, possess huge promotion to the reagent testing performance of chip.

In some prior art, the design of the rotary valve has more internal pipeline branches due to more complex structure, and the corresponding connecting pipeline of the matched chip is also very complex. This has two negative effects. Firstly, the complex three-dimensional pipeline design increases the difficulty for processing and manufacturing, reduces the yield and greatly improves the manufacturing cost; and secondly, excessive corners and connections increase the dead volume of the liquid reagent during flowing, enhance the carrying pollution, and cause difficulty in effective cleaning, and finally cause false negative of nucleic acid detection due to protein pollution or false positive of an immune detection result due to incomplete cleaning.

The liquid flow guide valve greatly reduces the number of pipelines and improves the operation efficiency of the valve, so that the number of guide channels is reduced to one. In the whole reaction process, the flow guide channel is repeatedly washed by subsequent washing liquor, and simultaneously, because the pipeline is simple, a large amount of reagent residues can not occur, so that the phenomenon that the pollution of the residual reactant influences the subsequent experiment is greatly reduced. The reaction efficiency is high, the detection result is accurate, and the negative background value is lower than that of other rotary valve designs.

It should be noted that the reagent storage bin, the primary reagent reaction bin, the secondary reagent reaction bin, the waste liquid bin and the like of the microfluidic chip can be adjusted and increased automatically according to the experiment and design requirements.

Some specific examples of flow directing valves and microfluidic chips are listed below.

In a particular embodiment, the rotational member 1 includes a first portion 14 that is circular and a second portion 15 that is circular. The first portion 14 has a diameter D of 17mm and a thickness of 4 mm. A trapezoidal slot is arranged at the center of the upper part and is used as an appliance matching part 16. One end of the trapezoidal clamping groove is 2.3mm wide, and the other end of the trapezoidal clamping groove is 1.7mm wide. The first portion 14 has a diameter of 23mm and a thickness of 2.4 mm.

The rotor 1 comprises a body 12 and a back plate 13. a counter bore of 0.4mm depth and 20mm diameter is bored inwardly from the side of the body 12 adjacent the back plate 13. A groove 121 with the depth of 0.5mm is designed from the center of the counter bore to the part close to the circumference, one end of the groove 121 is located at the center of the counter bore, the radius is 0.5mm, and the total length of the groove 121 is 8.5 mm. The other end of the groove 121 is also designed as a semicircle with a radius of 0.5 mm. In the diameter direction perpendicular to the extending direction of the groove 121, a positioning hole 122 is respectively disposed at a half radius of the center of the circle, and the positioning hole 122 is used for penetrating a positioning pin to position the back plate 13.

The back plate 13 is a polytetrafluoroethylene sheet with a diameter of 20mm and a thickness of 0.5 mm. Through holes with the diameter of 1mm are respectively arranged at the circle center and the position 7.5mm away from the circle center to be used as liquid flow holes. Two through holes are also respectively formed at the half radius positions on the two sides of the circle center in the direction vertical to the connecting line of the liquid flow hole to be used as positioning holes.

The overall diameter of the spacer 2 is 26mm and the thickness is 5 mm. A through hole is arranged in the center, and the diameter of the first through hole part is 17 mm. The diameter of the second through hole part is 23mm, and the depth is 2.5 mm.

In one embodiment, the overall size of the chip body 3 is 26mm 42mm 4 mm. A second flow path 32 directly communicating with the flow guide valve and a first flow path 31 communicating with the second flow path 32 through the flow guide valve are provided. The first flow hole 311 is spaced 7.5mm from the third flow hole 321.

The chip body 3 with the positioning structure 6 shown in fig. 9 has a radius of curvature of 4mm and a curvature angle of 82 °.

The chip body 3 with the positioning ring 5 is shown in fig. 10, the inner diameter of the positioning ring 5 is 23mm, the outer diameter is 26mm, the thickness is 2.3mm, and the outer side surface is provided with threads. Correspondingly, the positioning element 2 has an inner diameter of 26mm and an outer diameter of 29mm, and is provided on the inner side with a thread corresponding to the outer thread of the positioning ring 5 for locking.

In another embodiment, the overall size of the chip body 3 is 38mm 42mm 4 mm. Provided with a second flow path 32 directly communicating with the flow guide valve, and five first flow paths 31 communicating with the second flow path 32 through the flow guide valve. The five first flow holes 311 of the first flow channel 31 are distributed on a circumference 7.5mm from the third flow hole of the second flow channel 32.

In the chip body 3 with the light transmitting hole shown in fig. 12(a) and 12(b), one light transmitting hole is provided at a position 12 ° lateral to the first liquid flow hole 311 of the original first liquid flow channel 31 on the same circumference. Correspondingly, a light hole is correspondingly arranged on one side of the first diversion hole 111 of the diversion channel 11 on the rotating member 1.

The gasket 4 shown in FIG. 7 has an inner diameter of 17mm, an outer diameter of 23mm, and a thickness of 0.5 mm.

In the description of the present invention, it should be understood that the terms "first", "second", "third", etc. are used to define the components, and are used only for the convenience of distinguishing the components, and if not otherwise stated, the terms have no special meaning, and thus, should not be construed as limiting the scope of the present invention.

Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present invention and not to limit the same; although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art will understand that: modifications to the specific embodiments of the invention or equivalent substitutions for parts of the technical features may be made; without departing from the spirit of the present invention, it is intended to cover all aspects of the invention as defined by the appended claims.