Micropore blockage detection device and method and blood cell analyzer
阅读说明：本技术 微孔堵孔检测装置及方法、血液细胞分析仪 (Micropore blockage detection device and method and blood cell analyzer ) 是由 习武佳 李国军 于 2018-08-24 设计创作，主要内容包括：本发明公开一种微孔堵孔检测装置及检测方法、血液细胞分析仪。分析仪包括前池、后池、微孔、电压控制单元、压力源单元、流量监控单元、控制单元；压力源单元驱动前池含有血液细胞的液体经过微孔流入后池,引起电路单元电压信号变化获得脉冲信号进行血液细胞计数,同时流量监控单元进行监控,将经过流量监控单元的稳定流量设为预设阈值,在计数过程中实时检测流量并与预设阈值对比并根据对比结果判断是否发生了堵孔和堵孔时刻,本发明能够准确地判断堵孔发生时刻,直观反应堵孔严重程度,有效减少堵孔误判率。(The invention discloses a micropore blockage detection device and a detection method and a blood cell analyzer. The analyzer comprises a front pool, a rear pool, micropores, a voltage control unit, a pressure source unit, a flow monitoring unit and a control unit; the pressure source unit drives the liquid containing blood cells in the front pool to flow into the rear pool through the micropores, voltage signal changes of the circuit unit are caused to obtain pulse signals for blood cell counting, meanwhile, the flow monitoring unit monitors, stable flow passing through the flow monitoring unit is set as a preset threshold, the flow is detected in real time in the counting process and compared with the preset threshold, and whether hole plugging and hole plugging occur or not is judged according to a comparison result.)
1. A micropore plugging detection device, comprising:
the voltage control unit is connected with a positive electrode in the front cell and a negative electrode in the rear cell on two sides of the micropore and used for acquiring a voltage signal between the positive electrode and the negative electrode and converting the voltage signal into a pulse signal;
the pressure source unit is connected with the rear pool through a pipeline and provides suction force to enable liquid in the rear pool to flow to the pressure source unit so as to enable liquid containing blood cells in the front pool to flow to the rear pool;
the flow monitoring unit is arranged in the pipeline and used for measuring the liquid flow value passing through the pipeline when the liquid in the rear pool flows to the pressure source unit;
the control unit is connected with the flow monitoring unit and the voltage control unit and used for receiving pulse signals from the voltage control unit and calculating the number of blood cells according to the pulse signals, receiving a measured liquid flow value from the flow monitoring unit and comparing the measured liquid flow value with a preset threshold value, and sending an alarm signal when the measured liquid flow value is smaller than the preset threshold value; and
and the alarm unit is connected with the control unit and used for receiving the alarm signal from the control unit and giving an alarm to prompt the micropores to be blocked.
2. The micropore blockage detection device according to claim 1, wherein the preset threshold is a liquid flow value measured by the flow monitoring unit when no pore blockage occurs in the micropore.
3. The microporous plugging detection device of claim 1, wherein the pressure source unit comprises a pressure cell and a pressure pump, the pressure pump provides suction to the pressure cell to make the liquid in the rear cell flow to the pressure cell and further make the liquid containing blood cells in the front cell flow to the rear cell, so as to measure the liquid flow through the flow monitoring unit to indicate plugging.
4. The micropore blockage detection device according to claim 1, wherein the pressure source unit comprises a pressure cell and a pressure pump, the control unit outputs a first control signal to the voltage control unit and outputs a second control signal to the pressure pump, the voltage control unit burns the micropores through the positive electrode and the negative electrode according to the first control signal, and the pressure pump provides pressure to the pressure cell according to the second control signal so that the liquid in the pressure cell flows to the rear cell and further flows to the front cell through the micropores to remove the blockage.
5. A blood cell analyzer, comprising:
the forebay, contain conducting liquid and positive electrode containing blood cell;
the rear pool is connected with the front pool through micropores and is filled with conductive liquid and a negative electrode;
the voltage control unit is connected with the positive electrode and the negative electrode and used for acquiring a voltage signal between the positive electrode and the negative electrode and converting the voltage signal into a pulse signal;
the pressure source unit is connected with the rear pool through a pipeline and provides suction force to enable liquid in the rear pool to flow to the pressure source unit so as to enable liquid containing blood cells in the front pool to flow to the rear pool;
the flow monitoring unit is arranged in the pipeline and used for measuring the liquid flow value passing through the pipeline when the liquid in the rear pool flows to the pressure source unit;
the control unit is connected with the flow monitoring unit and the voltage control unit and used for receiving pulse signals from the voltage control unit and calculating the number of blood cells according to the pulse signals, receiving a measured liquid flow value from the flow monitoring unit and comparing the measured liquid flow value with a preset threshold value, and sending an alarm signal when the measured liquid flow value is smaller than the preset threshold value; and
and the alarm unit is connected with the control unit and used for receiving the alarm signal from the control unit and giving an alarm to prompt the micropores to be blocked.
6. The hematology analyzer of claim 5, wherein the predetermined threshold is a liquid flow value measured by the flow monitoring unit when the micro-hole is not plugged, the front cell and the rear cell are both non-conductive containers, and the conductive liquid is a cell suspension diluted by an isotonic electrolyte solution.
7. The blood cell analyzer of claim 5, wherein the pressure source unit comprises a pressure cell and a pressure pump, the pressure pump provides suction to the pressure cell to make the liquid in the rear cell flow to the pressure cell and further make the liquid containing blood cells in the front cell flow to the rear cell, so as to measure the liquid flow through the flow monitoring unit to indicate the hole blockage.
8. The blood cell analyzer of claim 5, wherein the pressure source unit comprises a pressure cell and a pressure pump, the control unit outputs a first control signal to the voltage control unit and a second control signal to the pressure pump, the voltage control unit burns the micropores through the positive electrode and the negative electrode according to the first control signal, and the pressure pump provides pressure to the pressure cell according to the second control signal to enable the liquid in the pressure cell to flow to the rear cell and further to enable the liquid in the rear cell to flow to the front cell through the micropores to remove the blocked pores.
9. A method for detecting pore blockage in a micropore, the method comprising:
acquiring a voltage signal between a positive electrode in the front cell and a negative electrode in the rear cell through a voltage control unit and converting the voltage signal into a pulse signal;
providing suction through a pressure source unit to enable the liquid in the rear pool to flow to the pressure source unit so as to enable the liquid containing the blood cells in the front pool to flow to the rear pool;
measuring, by a flow monitoring unit, a liquid flow value through the pipeline as liquid in the rear sump flows to the pressure source unit;
receiving, by a control unit, a pulse signal from the voltage control unit and calculating a blood cell count from the pulse signal;
receiving, by a control unit, a measured liquid flow value from the flow monitoring unit and comparing the measured liquid flow value to a preset threshold value; and
when the measured liquid flow value is smaller than the preset threshold value, an alarm signal is sent out through the control unit;
and alarming through an alarm unit to prompt the micropores to be blocked.
10. The microporous plugging detection method of claim 9, wherein said receiving, by a control unit, a measured liquid flow value from the flow monitoring unit and comparing the measured liquid flow value to a preset threshold value comprises:
when the measured liquid flow value received by the control unit is smaller than the preset threshold value, the micropores are subjected to micro-plugging; and
when the measured liquid flow value received by the control unit is equal to zero, the micropores are subjected to full pore blocking;
when the measured liquid flow value is smaller than the preset threshold value, alarming to prompt the micropores to block the pores, and then further comprising:
outputting a first control signal to the voltage control unit through the control unit;
firing the micropores through the voltage control unit, the positive electrode and the negative electrode;
outputting a second control signal to the pressure pump of the pressure source unit through the control unit;
providing pressure to the pressure cell by the pressure pump so that liquid in the pressure cell flows to the rear cell through the pipeline; and
and controlling the liquid in the rear pool to flow to the front pool to remove the blocked holes.
Technical Field
The invention relates to the technical field of medical treatment, in particular to a micropore plugging detection method and device for medical equipment and a blood cell analyzer.
Background
Blood cell analysis has been widely used in the medical field of in vitro diagnosis, and becomes a clinical routine detection device. The blood cell count in the blood cell analyzer generally adopts an electrical impedance method, utilizes an electric pulse signal generated by the flow of blood cells from a front pool to a gem micropore between back pools, and counts the blood cells in a pulse identification mode. In the working process, because the pore diameter of the gem micropore is small, and some protein and cell debris in blood can be adhered to the periphery of the gem micropore, the pore blocking phenomenon of the gem micropore is difficult to avoid. Abnormal plugging of the gemstone's micropores can affect the stability of the results and the reliability of the instrument. The hole blocking phenomenon can be divided into two conditions according to the time point of occurrence, the first hole blocking condition is that the hole blocking occurs in the counting process, the second hole blocking condition is that the hole blocking occurs before counting, and the hole blocking can be divided into complete blocking and micro blocking according to the severity of the hole blocking.
In the prior art, three methods are mainly used for judging the hole blockage of the micropore of the gem. The first method is a volumetric method, in which a liquid volumetric sensor is added to a pipeline, and whether a hole is blocked during measurement is determined by the arrival of liquid at a position sensor within a predetermined time. The second method is to judge whether the hole blockage occurs or not through the stability of the particle flow in the counting process, and the method has certain judgment capability on the hole blockage problem in the counting process, but the abnormal hole blockage condition that micro-blockage occurs before counting and the particle flow is in a stable state in the counting process is difficult to judge, so the method also needs to be improved. The third method is to identify whether the voltage signal has sudden change or not by presetting the threshold value of the differential voltage signal and carrying out characteristic analysis on the voltage signal of the micropore of the gemstone, and detect whether the micropore has hole blockage or not by combining the moment when the sudden change occurs in the voltage signal is identified, and the method can identify the severity of the hole blockage and the time point when the hole blockage occurs to a certain extent, but has obvious defects: firstly, the setting of the threshold of the differential voltage signal is greatly influenced by the concentration of the sample and whether the sample is an abnormal sample, namely, the voltage signal mutation characteristics of the samples with different concentrations under the condition that the hole blocking actually occurs are different, at the moment, the accuracy is difficult to evaluate by using the threshold of the differential voltage signal, and secondly, the voltage signal generated by the actual blood cell sample through the micropore is easily influenced by factors such as the conductivity, the temperature, the liquid flow and the physical parameters of the micropore itself of the solution at the two ends of the micropore, and the above defects can cause certain hole blocking misjudgment rate.
Disclosure of Invention
The invention mainly solves the technical problem of providing a micropore plugging detection device and method and a blood cell analyzer, so as to achieve the purposes of accurately judging the occurrence time of plugging holes, visually reflecting the severity of the plugging holes and effectively reducing the misjudgment rate of the plugging holes.
In order to solve the technical problems, the invention adopts a technical scheme that: provided is a micropore plugging detection device, comprising:
the voltage control unit is connected with a positive electrode in the front cell and a negative electrode in the rear cell on two sides of the micropore and used for acquiring a voltage signal between the positive electrode and the negative electrode and converting the voltage signal into a pulse signal;
the pressure source unit is connected with the rear pool through a pipeline and provides suction force to enable liquid in the rear pool to flow to the pressure source unit so as to enable liquid containing blood cells in the front pool to flow to the rear pool;
the flow monitoring unit is arranged in the pipeline and used for measuring the liquid flow value passing through the pipeline when the liquid in the rear pool flows to the pressure source unit;
the control unit is connected with the flow monitoring unit and the voltage control unit and used for receiving pulse signals from the voltage control unit and calculating the number of blood cells according to the pulse signals, receiving a measured liquid flow value from the flow monitoring unit and comparing the measured liquid flow value with a preset threshold value, and sending an alarm signal when the measured liquid flow value is smaller than the preset threshold value; and
and the alarm unit is connected with the control unit and used for receiving the alarm signal from the control unit and giving an alarm to prompt the micropores to be blocked.
In order to solve the technical problem, the invention adopts another technical scheme that: provided is a micropore plugging detection method, comprising the following steps:
acquiring a voltage signal between a positive electrode in the front cell and a negative electrode in the rear cell through a voltage control unit and converting the voltage signal into a pulse signal;
providing suction through a pressure source unit to enable the liquid in the rear pool to flow to the pressure source unit so as to enable the liquid containing the blood cells in the front pool to flow to the rear pool;
measuring, by a flow monitoring unit, a liquid flow value through the pipeline as liquid in the rear sump flows to the pressure source unit;
receiving, by a control unit, a pulse signal from the voltage control unit and calculating a blood cell count from the pulse signal;
receiving, by a control unit, a measured liquid flow value from the flow monitoring unit and comparing the measured liquid flow value to a preset threshold value; and
when the measured liquid flow value is smaller than the preset threshold value, an alarm signal is sent out through the control unit;
and alarming through an alarm unit to prompt the micropores to be blocked.
In addition, the present invention also provides a blood cell analyzer comprising:
the forebay, contain conducting liquid and positive electrode containing blood cell;
the rear pool is connected with the front pool through micropores and is filled with conductive liquid and a negative electrode;
the voltage control unit is connected with the positive electrode and the negative electrode and used for acquiring a voltage signal between the positive electrode and the negative electrode and converting the voltage signal into a pulse signal;
the pressure source unit is connected with the rear pool through a pipeline and provides suction force to enable liquid in the rear pool to flow to the pressure source unit so as to enable liquid containing blood cells in the front pool to flow to the rear pool;
the flow monitoring unit is arranged in the pipeline and used for measuring the liquid flow value passing through the pipeline when the liquid in the front pool flows to the rear pool through the micro-holes and the liquid in the rear pool flows to the pressure source unit;
the control unit is connected with the flow monitoring unit and the voltage control unit and used for receiving pulse signals from the voltage control unit and calculating the number of blood cells according to the pulse signals, receiving a measured liquid flow value from the flow monitoring unit and comparing the measured liquid flow value with a preset threshold value, and sending an alarm signal when the measured liquid flow value is smaller than the preset threshold value; and
and the alarm unit is connected with the control unit and used for receiving the alarm signal from the control unit and giving an alarm to prompt the micropores to be blocked.
The invention has the beneficial effects that: the invention provides a micropore plugging detection device and a method which are different from the prior art.A blood cell analyzer comprises a voltage control unit which is connected with a positive electrode in a front pool and a negative electrode in a rear pool at two sides of a micropore; the pressure source unit is connected with the rear pool through a pipeline and provides suction force to enable the cell liquid in the front pool to flow to the rear pool; the flow monitoring unit is arranged in the pipeline and is used for detecting the flow value in the pipeline when the cell sap flows to the pressure source unit from the rear pool; and the control unit is connected with the flow monitoring unit and the voltage control unit and used for receiving pulse signals from the voltage control unit, calculating the number of blood cells according to the pulse signals, receiving a measured liquid flow value from the flow monitoring unit, comparing the measured liquid flow value with a preset threshold value, and sending an alarm signal to an alarm unit when the measured liquid flow value is smaller than the preset threshold value so as to alarm and prompt the micropores to be blocked, so that the time point of the occurrence of the blocked micropores can be accurately judged, the severity of the blocked pores can be intuitively reflected, and the misjudgment rate of the blocked pores is effectively reduced.
Drawings
FIG. 1 is a schematic structural view of a microporous clogging detection apparatus and a blood cell analyzer according to the present invention;
FIGS. 2 and 3 are schematic views showing the flow of the method for detecting the clogging of the micropores according to the present invention;
FIG. 4a is a schematic diagram illustrating the variation of the liquid flow rate detected by the flow rate monitoring unit in the microporous plugging detection device according to the present invention;
FIG. 4b is a schematic diagram of a waveform of a sample particle flow corresponding to a preset threshold in the micropore blockage detecting device according to the present invention;
fig. 4 c-4 e are schematic waveforms of the liquid flow rate detected by the flow rate monitoring unit and the sample particle flow corresponding to the liquid flow rate in the microporous plugging detection device according to the present invention.
Detailed Description
The present invention will be described in detail below with reference to the accompanying drawings and examples.
Fig. 1 is a schematic structural diagram of a micropore blockage detection device according to the present invention. The micropore
the
a
a flow
a
and the
Wherein the preset threshold is a liquid flow value measured by the
The
The
The pressure source unit includes a
When the power is turned on, the
After the
Fig. 1 is a schematic structural diagram of the blood cell analyzer of the present invention. The
a
the rear pool 2 is connected with the
micropore plugging
a
a
a flow
a
and the
The preset threshold is a liquid flow value measured by the
The
Wherein the diameter of the
In this embodiment, the
The blood cell analyzer adopts the Coulter impedance method counting principle, namely, cell sap diluted by isotonic electrolyte solution is placed in a non-conductive
After the
Referring to fig. 2, a schematic flow chart of the method for detecting the pore blockage of the micropores according to the present invention is shown. The method comprises the following steps:
step S1: and acquiring a voltage signal between a positive electrode in the front cell and a negative electrode in the rear cell through a voltage control unit and converting the voltage signal into a pulse signal.
Step S2: and providing suction through a pressure source unit to enable the liquid in the rear pool to flow to the pressure source unit so as to enable the liquid containing the blood cells in the front pool to flow to the rear pool.
Step S3: measuring, by a flow monitoring unit, a liquid flow value through the conduit as liquid in the rear sump flows to the pressure source unit.
The flow monitoring unit can be a micro-liter flow meter or a flow digital display device with high precision.
Step S4: receiving, by a control unit, a pulse signal from the voltage control unit and calculating a blood cell count from the pulse signal.
Step S5: receiving, by a control unit, a measured liquid flow value from the flow monitoring unit and comparing the measured liquid flow value to a preset threshold value.
Wherein the preset threshold is a liquid flow value measured by the flow monitoring unit when the micropore is not blocked.
Step S6: and when the measured liquid flow value is smaller than the preset threshold value, sending an alarm signal through a control unit.
Step S7: and receiving the alarm signal through an alarm unit and giving an alarm to prompt the micropores to block the pores.
The alarm unit may be a voice alarm device, such as a warning tone, or a display alarm device, such as a red display, for alarming.
Wherein, step S6 includes: when the measured liquid flow value received by the control unit is smaller than the preset threshold value, the micropores are subjected to micro-plugging;
and when the measured liquid flow value received by the control unit is equal to zero, the micropores are subjected to full pore blocking.
Referring to fig. 3, after step S7, the method further includes:
step S8: and outputting a first control signal to the voltage control unit through the control unit.
Step S9: and burning the micropores through the voltage control unit, the positive electrode and the negative electrode.
The positive electrode and the negative electrode are positioned at two sides of the micropore, and voltage is provided for the positive electrode and the negative electrode through the voltage control unit so as to charge the positive electrode and the negative electrode, so that the micropore is burned, and impurities, such as protein and the like, positioned around the micropore are dispersed.
Step 10: and outputting a second control signal to the pressure pump of the pressure source unit through the control unit.
Step S11: and providing pressure to the pressure pool through the pressure pump so that the liquid in the pressure pool flows to the rear pool through the pipeline.
Step S12: and controlling the liquid in the rear pool to flow to the front pool to remove the blocked holes.
Please refer to fig. 4a and fig. 4b, which are schematic diagrams illustrating a predetermined threshold Q1 and a corresponding sample particle flow, wherein Q1 is a predetermined threshold, i.e., a stable flow value;
referring to fig. 4a and 4c, in order to monitor the flow rate Q2 and the corresponding sample particle flow in real time, the flow rate Q2 is smaller than the preset threshold Q1 at the time T2, so that it can be determined that the micro plugging of the
Referring to fig. 4a and 4d, in order to monitor the flow rate Q3 and the corresponding sample particle flow in real time, the flow rate Q3 is smaller than the preset threshold Q1 at the time T0, so that it can be determined that the micro-plugging occurs before the time T0.
Referring to fig. 4a and 4e, in order to monitor the flow rate Q4 and the corresponding sample particle flow in real time, the flow rate Q4 is zero at the time T1, so that it can be determined that the micro-hole 3 is completely blocked at the time T1.
In this embodiment, the micropore blockage detection device only describes a part of related functional units, and other functional units are the same as the functional units of the micropore blockage detection device in the prior art, and are not described herein again.
The micropore blockage detection device and method and the blood cell analyzer acquire a voltage signal between a positive electrode in a front cell and a negative electrode in a rear cell through a voltage control unit and convert the voltage signal into a pulse signal; providing suction through a pressure source unit to enable the liquid in the rear pool to flow to the pressure source unit so as to enable the liquid containing the blood cells in the front pool to flow to the rear pool; measuring, by a flow monitoring unit, a liquid flow value through the pipeline as liquid in the rear sump flows to the pressure source unit; receiving, by a control unit, a pulse signal from the voltage control unit and calculating a blood cell count from the pulse signal; receiving, by a control unit, a measured liquid flow value from the flow monitoring unit and comparing the measured liquid flow value to a preset threshold value; when the measured liquid flow value is smaller than the preset threshold value, an alarm is given to prompt the micropores to block the holes, so that the time point of the occurrence of the hole blocking can be accurately judged, the severity of the hole blocking can be intuitively reflected, and the misjudgment rate of the hole blocking is effectively reduced.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes performed by the present specification and drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
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