阅读说明：本技术 红车轴草提取物用作酪氨酸酶抑制剂的用途 (Use of red clover extract as tyrosinase inhibitor ) 是由 熊登科 黄志军 吴木琴 向阳 熊莉 曾庆恢 刘�英 吴保明 宋畅 姚润 卢敏 于 2019-12-09 设计创作，主要内容包括：本发明公开了红车轴草提取物用作酪氨酸酶抑制剂的用途。体外酶活试验结果表明：红车轴草水提取物和不同浓度乙醇提取物对酪氨酸酶均有一定的抑制活性,因此,红车轴草提取物是一种优良的酪氨酸酶天然抑制剂,有进一步开发成美容化妆品或皮肤用药的潜力。(The invention discloses an application of an red clover extract as a tyrosinase inhibitor. The in vitro enzyme activity test result shows that: the red clover water extract and the ethanol extracts with different concentrations have certain inhibitory activity on tyrosinase, so the red clover extract is an excellent tyrosinase natural inhibitor and has the potential of further developing into beauty cosmetics or skin medicaments.)

1. Use of Trifolium pratense extract as tyrosinase inhibitor is provided.

2. A tyrosinase inhibitor contains Trifolium Pratense L extract as active ingredient.

3. The tyrosinase inhibitor of claim 2, wherein: the red clover extract is an alcohol extract of red clover.

4. The tyrosinase inhibitor of claim 3, wherein: the alcohol is 20-80% ethanol.

5. The tyrosinase inhibitor of claim 4, wherein: the alcohol is 40% ethanol.

6. A cosmetic containing a whitening agent, characterized in that: the whitening agent is red clover extract.

7. A medicament for treating diseases caused by melanin pigmentation, which is characterized in that: the active component of the medicine is red clover extract.

8. The medicament of claim 7, wherein: the diseases caused by melanin pigmentation include freckle, chloasma, and melanoma.

9. The cosmetic according to claim 6, or the pharmaceutical according to claim 7 or 8, characterized in that: the red clover extract is an alcohol extract of red clover, especially 20-80% ethanol extract, most preferably 40% ethanol extract.

Technical Field

The invention relates to a new application of an red clover extract, in particular to a new application of the red clover extract as a tyrosinase inhibitor.

Background

Trifolium pratense L is a Trifolium plant of Trifolium of Papilionaceae of Leguminosae, and is a perennial herb, also called clover, red jojoba seesaw, etc. The red clover is distributed all over the world, is mainly scattered in the areas of Hubei, Xinjiang, Yunnan and the like in China, and has abundant resources. The red clover inflorescence is used as a medicine in China, is mainly used for relieving cough, relieving asthma, relieving spasm and the like, and can be used for treating local ulcer after being prepared into ointment. The plant mainly contains isoflavone, flavone, coumarin, organic acid, etc., and the isoflavone has estrogen-like effect and can regulate hormone level in human body, so as to treat gynecological diseases related to estrogen level disorder such as climacteric syndrome and osteoporosis. With the continuous and intensive research on the chemical components and pharmacological activity of red clover, the red clover has certain effects of resisting cancer, resisting malaria, eliminating phlegm, relieving spasm and the like, and can be used for treating diseases such as pertussis, bronchitis and the like.

Tyrosinase (Tyrosinase) is an oxidase, is a rate-limiting enzyme for regulating melanin generation, plays a vital role in the melanin generation process, can catalyze L-tyrosine to synthesize L-dopa, then the dopa is oxidized to generate dopaquinone, and further pigments are formed, the overexpression of the dopaquinone in a human body is a main reason for pigmentation such as freckles, chloasma and melanoma, and among the currently used decolouring agents, hydroquinone, kojic acid, a mercury-containing preparation, hydrogen peroxide and the like are substances with high toxicity, so that the search for a natural and effective Tyrosinase inhibitor is a research hotspot of whitening cosmetics and skin drugs.

Disclosure of Invention

An object of the present invention is to provide a novel use of red clover extract as tyrosinase inhibitor.

The in vitro enzyme activity test result shows that: the red clover water extract and the ethanol extracts with different concentrations have certain inhibitory activity on tyrosinase, wherein the activity of the ethanol extract is higher than that of the water extract, and the activity of the 40% ethanol extract is close to that of chamomile.

The above results show that: the red clover extract is an excellent tyrosinase natural inhibitor and has the potential of further developing into cosmetics or skin medicaments.

It is another object of the present invention to provide a tyrosinase inhibitor.

The red clover extract can be used as an active ingredient for a tyrosinase inhibitor independently, and can also be used as an active ingredient for the tyrosinase inhibitor together with other ingredients with the same activity, such as chamomile extract, arbutin and the like, so that the tyrosinase inhibitory activity is further improved. The tyrosinase inhibitor contains active ingredients and pharmaceutically acceptable carriers.

Preferably, the red clover extract is a red clover alcohol extract, and the alcohol includes, but is not limited to, ethanol, methanol, propylene glycol, n-butanol, isobutanol and other monohydric or polyhydric alcohols.

The present invention is preferably 20-80% ethanol.

Further preferred according to the invention is 40% ethanol.

Based on the invention, the invention further provides a cosmetic or daily chemical product, wherein the cosmetic or daily chemical product contains the whitening agent, and the whitening agent is the red clover extract. The cosmetics comprise dosage forms of facial masks, milky lotions, creams and the like, and the daily chemical products comprise dosage forms of perfumed soaps, shower gels and the like. The cosmetic or daily chemical product has a main function of whitening.

Based on the above invention, the present invention still further provides a medicament for treating diseases caused by melanin pigmentation, the active ingredient of which is red clover extract. The diseases caused by the melanin pigmentation include but are not limited to freckles, chloasma and melanoma.

Of course, the application of the invention is not limited to this, and in view of the wide existence and important role of tyrosinase in bacteria, microorganisms, plants and animals, the inhibitor provided by the invention also has great application potential in the fields of agriculture, animal husbandry, food additives and the like, and the application related to the tyrosinase inhibitor is within the protection scope of the invention.

For a more detailed description, reference is made to the examples.

Detailed Description

The present invention will be described in detail below with reference to specific examples.