阅读说明：本技术 一种快速培养自养-异养生物脱氮颗粒污泥的装置及方法 (Device and method for rapidly culturing autotrophic-heterotrophic organism denitrification granular sludge ) 是由 王朝朝 高鹏 李思敏 闫立娜 赵丹 武新娟 殷春雨 于 2019-11-30 设计创作，主要内容包括：本发明公开了一种快速培养自养-异养生物脱氮颗粒污泥的装置及方法,该装置包括：配水箱通过设有提升泵的管路与高位水箱相连,高位水箱设出水口,与UASB-MBR反应器进水口相连,UASB反应器内设有上端分别与产水箱和化学反洗箱相连通的MBR膜组件；培养方法：一、接种颗粒和絮状厌氧氨氧化污泥通入人工合成废水；二、改变进水底物浓度,并进行间歇式微氧曝气培养,得到AOB-ANAMMOX颗粒污泥；三、人工合成废水中引入有机碳源,使底物浓度C/N比为1,间歇式微氧曝气培养,得到自养-异养生物脱氮颗粒污泥；本发明有效防止了絮状功能菌群培养过程中的流失,提供良好的微生物生长所需环境,可以快速培养并使AOB、ANAMMOX和DNB高效耦合,快速培养出自养-异养生物脱氮颗粒污泥。(The invention discloses a device and a method for rapidly culturing autotrophic-heterotrophic organism denitrification granular sludge, wherein the device comprises: the water distribution tank is connected with a high-level water tank through a pipeline provided with a lift pump, the high-level water tank is provided with a water outlet and is connected with a water inlet of the UASB-MBR reactor, and an MBR membrane component with the upper end respectively communicated with the water production tank and the chemical backwashing tank is arranged in the UASB reactor; the culture method comprises the following steps: firstly, introducing the inoculated particles and flocculent anaerobic ammonium oxidation sludge into artificially synthesized wastewater; changing the concentration of a water inlet substrate, and carrying out intermittent micro-aerobic aeration culture to obtain AOB-ANAMMOX granular sludge; introducing an organic carbon source into the artificially synthesized wastewater to enable the concentration C/N ratio of a substrate to be 1, and carrying out intermittent micro-aerobic aeration culture to obtain autotrophic-heterotrophic organism denitrification granular sludge; the invention effectively prevents loss in the process of culturing flocculent functional flora, provides good environment for the growth of microorganisms, can be quickly cultured, enables AOB, ANAMMOX and DNB to be efficiently coupled, and quickly cultures the autotrophic-heterotrophic organism denitrification granular sludge.)

1. The utility model provides a cultivate autotrophy-heterotrophic biological denitrogenation granule mud device fast, includes water distribution tank (1), overhead tank (2), UASB-MBR reactor (3), produces water tank/produces water backwash box (5) and chemical backwash water tank (6), its characterized in that: the water distribution tank (1) is connected with the high-level water tank (2) through a pipeline provided with a high-level water tank lifting pump (12), a water outlet arranged at the bottom of the high-level water tank (2) is connected with a water inlet (26) arranged at the bottom of the UASB-MBR reactor (3), an MBR membrane component (8) is arranged at the middle part of the UASB-MBR reactor (3), the upper end of the MBR membrane component (8) is respectively connected with a produced water/produced water backwashing tank (5) and a chemical backwashing tank (6) through pipelines provided with a produced water/produced water backwashing pump (18) and a chemical backwashing pump (17), an internal circulation port a (28) arranged at the upper end of the UASB-MBR reactor (3) is connected with an internal circulation port b (28) at the bottom of the UASB-MBR reactor (3) through a pipeline provided with an internal circulation pump (16), a water area (9) is arranged outside the UASB-MBR reactor (3), the water area sleeve (9) is connected with the water area circulating water tank (4) through a pipeline provided with a water area circulating pump (15), and a heating rod (22) is arranged in the water area circulating water tank (4).

2. The apparatus for rapid culture of granular sludge for denitrification of autotrophic-heterotrophic organisms according to claim 1, wherein: the high-level water tank lift pump (12) continuously introduces the artificial synthetic wastewater in the water distribution tank (1) into the high-level water tank (2), the UASB-MBR reactor (3) produces water through an MBR membrane component (8) provided with a water production/water production backwashing pump (18) and a chemical backwashing pump (17), and the artificial synthetic wastewater in the high-level water tank (2) is continuously supplemented into the UASB-MBR reactor (3).

3. The apparatus for rapid culture of granular sludge for denitrification of autotrophic-heterotrophic organisms according to claim 1, wherein: the effective volume of the UASB-MBR reactor (3) is 4.5L; the front surface of the UASB-MBR reactor (3) is provided with a sampling port (25) at intervals of 25cm, 3 sampling ports are arranged, a pipe is arranged on the 3# (25) of the sampling port, a pH/DO value probe (10) is arranged in a vertical pipe, and the signal output end of the pH/DO value probe (10) is electrically connected with the signal input end of a WTW water quality analyzer (11); the bottom of the UASB-MBR reactor (3) is in an inverted cone shape, the bottom is provided with a water inlet (26), an aeration head (27), an internal circulation port b (28) and a sludge taking port (29), and the aeration head (27) is connected with an aeration pump (13) through a pipeline provided with a gas flowmeter (14).

4. The apparatus for rapid culture of granular sludge for denitrification of autotrophic-heterotrophic organisms according to claim 1, wherein: the effective membrane filament area of the MBR membrane component (8) is 0.075 m²The material is PVDF and is positioned one third away from the top of the UASB-MBR reactor (3); and a water production pressure gauge (21) is arranged on a water production pipeline of the MBR membrane module (8) and the water production/water production backwashing pump (18).

5. The apparatus for rapid culture of granular sludge for denitrification of autotrophic-heterotrophic organisms according to claim 1, wherein: produce water tank/produce water backwash case (5) and set up the water inlet, produce water backwash mouth and overflow mouth, produce water/produce water backwash pump (18) and be two-way pump, link to each other with product water pipeline and product water backwash pipeline to produce the water direction and be forward, produce water backwash direction and be reverse, produce water operating mode, open and produce water solenoid valve (20), close and produce water backwash solenoid valve (19), produce water backwash operating mode, open and produce water backwash solenoid valve (19), close and produce water solenoid valve (20).

6. The apparatus for rapid culture of granular sludge for denitrification of autotrophic-heterotrophic organisms according to claim 1, wherein: the output ends of the high-level water tank lifting pump (12), the aeration pump (13), the water area circulating pump (15), the internal circulating pump (16), the chemical backwashing pump (17) and the water production/water production backwashing pump (18) are all in electric signal connection with the input end of the PLC control device (23), and the output end of the PLC control device (23) is in electric signal connection with the input end of the PC computer (24).

7. The apparatus for rapid culture of granular sludge for autotrophic-heterotrophic biological denitrification according to any one of claims 1 to 6, wherein the granular sludge for autotrophic-heterotrophic biological denitrification is cultured by the following method:

step one, inoculating 1000 ml of anaerobic ammonia oxidation flocculent sludge and 500 ml of anaerobic ammonia oxidation granular sludge in a UASB-MBR reactor, and then introducing NH₄ ⁺-N concentration 70 + -10 mg/L, NO₂ ^-Artificial synthetic wastewater with the N concentration of 90 +/-10 mg/L, controlling the temperature of the reactor to be 33 +/-2.0 ℃ in a water bath circulation manner, and controlling the rising flow rate to be 7.5 m/h; firstly, the hydraulic retention time is 24 h, and the water flux produced by the membrane is 2.5L/(m)²H) running for 20 d, then shortening the hydraulic retention time to 12 h, and ensuring that the water flux produced by the membrane is 5.0L/(m)²H) running for 20 d, then continuing to shorten the hydraulic retention time to 6.0 h, and the water flux produced by the membrane is 10L/(m)²H) running for 20 d, the final hydraulic retention time is 4.0h, and the water flux produced by the membrane is 15L/(m)²H) run 20 d;

step two, carrying out intermittent aeration in a UASB-MBR reactor, wherein the aeration stop ratio is 4.0: 10, aeration amount is 50 mL/min, dissolved oxygen concentration is controlled to be 0.2-0.5mg/L, water bath circulation control reactor temperature is 33 +/-2.0 ℃, rising flow rate is 8.0 m/h, hydraulic retention time is 6.0 h, and membrane water production flux is 10L/(m/m)²H); first with NH₄ ⁺N concentration of 200. + -.10 mg/L, NO₂ ^-Running the artificial synthetic wastewater with the N concentration of 100 +/-10 mg/L for 15 days; then with NH₄ ⁺N concentration of 200. + -.10 mg/L, NO₂ ^-The artificial synthetic wastewater with the N concentration kept at 60 +/-10 mg/L runs for 15 days; then with NH₄ ⁺N concentration of 200. + -.10 mg/L, NO₂ ^-The artificial synthetic wastewater with the N concentration kept at 20 +/-5.0 mg/L runs for 15 days; obtaining AOB-ANAMMOX granular sludge;

step three, introducing an organic carbon source into the artificial synthetic wastewater, wherein the substrate concentrations of the artificial synthetic wastewater are respectively NH₄ ⁺N concentration of 200. + -.10 mg/L, NO₂ ^-Keeping the N concentration at 20 +/-5.0 mg/L, keeping the COD concentration at 200 +/-15 mg/L, continuously carrying out intermittent aeration on a UASB-MBR reactor, and keeping the aeration stop ratio at 4.0: 10, the aeration rate is 50 mL/min, and the dissolved oxygen concentration is controlled to be 0.2-0.5mg/L, the temperature of the reactor is controlled by water bath circulation to be 33 +/-2.0 ℃, the rising flow rate is 8.5 m/h, the hydraulic retention time is 6.0 h, and the water flux produced by the membrane is 10L/(m)²H) culturing for 37 d under the condition of the sludge, thereby obtaining autotrophic-heterotrophic organism denitrification granular sludge;

detecting the concentrations of nitrate nitrogen, nitrite nitrogen, ammonia nitrogen and COD in inlet water and outlet water every day in the culture process, and calculating the removal rate of each nitrogen-containing index; recording the transmembrane pressure difference of the produced water; along with the stage change, the particle size of the granular sludge is gradually increased, and the ascending flow velocity is gradually increased.

8. The method for rapid culture of granular sludge for denitrification of autotrophic-heterotrophic organisms according to claim 7, wherein: the main component of the artificial synthetic wastewater contains NH₄Cl、NaNO₂、NaHCO₃、KH2PO₄、MgSO₄·7H₂O、CaCl₂·2H₂O、FeSO₄、C₂H₃NaO₂(ii) a NaHCO with different concentrations in different stages of ammonia nitrogen and nitrite concentration₃The concentration is 500mg/L, KH₂PO₄The concentration is 27.2 mg/L, CaCl₂·2H₂O concentration of 180 mg/L, MgSO₄·7H₂The concentration of O is 300 mg/L; trace element i (g/L): 1.25 KHCO₃，0.025 KH₂PO₄，0.3 CaCl₂·2H₂O，0.2 MgSO₄·7H₂O，0.00625FeSO₄(ii) a Microelement II (g/L): 15 EDTA, 0.43 ZnSO₄·7H₂O，0.24 CoCl₂·6H₂O，0.99 MnCl₂·4H₂O，0.25 CuSO₄·5H₂O，0.22 NaMoO₄·2H₂O，0.19 NiCl₂·6H₂O，0.21 NaSeO₄·10H₂O，0.014 H₃BO₄，0.05 NaWO₄·2H₂O; the dosage of the trace elements I and II is 1.0 mL/L.

9. The method for rapid culture of granular sludge for denitrification of autotrophic-heterotrophic organisms according to claim 7, wherein: the organic carbon source is anhydrous sodium acetate.

10. The method for rapid culture of granular sludge for denitrification of autotrophic-heterotrophic organisms according to claim 7, wherein: the water production/water production backwashing pump (18) and the chemical backwashing pump (17) take 10 min as a period, the water production/water production backwashing pump (18) and the chemical backwashing pump (17) alternately operate, and the water production flux of the operation of the MBR membrane component (8) is 2.5-15L/(m & lt m & gt)²H) backwash flux of 3.5-22.5L/(m)²H), the solution in the chemical backwashing water tank (6) is a sodium hypochlorite solution, the concentration of the solution is 0.5%, the water production/water production backwashing pump (18) operates for 9.0 min under the water production working condition, the water production/water production backwashing pump (18) stops, the chemical backwashing pump (17) operates for 30 s, and one period is completed.

Technical Field

The invention relates to the technical field of biological denitrification of sewage, in particular to a device and a method for quickly culturing autotrophic-heterotrophic biological denitrification granular sludge.

Background

At present, the problem of slow-flow water eutrophication caused by nitrogen is increasingly serious, global attention is paid, and how to economically and efficiently remove the nitrogen becomes a research hotspot in the industry. The traditional biological denitrification process mainly depends on aerobic Ammonia Oxidizing Bacteria (AOB) and Nitrobacteria (NOB) to complete nitration reaction, and then depends on denitrifying bacteria (DNB) to complete denitrification reaction, thereby realizing the transfer of nitrogen from a water phase to a gas phase and achieving the purpose of removing ammonia nitrogen in sewage/wastewater. Because the denitrification process needs to provide a carbon source as an electron donor, the traditional biological denitrification process is greatly limited in the field of low C/N ratio sewage treatment.

ANaerobic AMMonium OXidation (ANAMMOX) is the most economical new biological autotrophic denitrification technology known at present, and compared with the traditional biological denitrification process, the autotrophic denitrification process has the advantages of low energy consumption, no need of additional carbon source, low sludge yield and the like. However, anaerobic ammonia oxidation can only remove 90% of nitrogen in the form of ammonia and nitrous acid, and about 10% of nitrogen is converted into nitrate nitrogen and cannot be removed; most practical sewage contains both organic carbon sources and nitrogen sources, and organic matters with different concentrations in the sewage can inhibit the anammox bacteria to different degrees. In recent years, researches show that anaerobic ammonium oxidation bacteria and denitrifying bacteria can coexist in the same reactor and form a certain synergistic effect, and a new method for synchronous denitrification and decarbonization of nitrogen-containing organic wastewater is provided. The method is an effective method for improving the nitrogen removal efficiency of nitrogen-containing organic sewage by combining autotrophic bacteria and heterotrophic bacteria by realizing synchronous nitrosation and anaerobic ammonia oxidation coupled heterotrophic Denitrification (SNAD) in a single-stage reactor.

The autotrophic-heterotrophic organism denitrified granular sludge is formed by the coexistence and coupling of anaerobic ammonium oxidation bacteria, ammonia oxidation bacteria and denitrifying bacteria, each functional bacteria has different optimal growth environments, and the phenomena of substrate competition and activity inhibition can occur in the culture process. In addition, the anaerobic ammonium oxidation bacteria belong to autotrophic anaerobes, and have slow growth rate, long generation time and easy loss. The effective retention of the anaerobic ammonium oxidation bacteria becomes the key of the success of the culture of the autotrophic-heterotrophic organism denitrified granular sludge. The Membrane Bioreactor (MBR) process depends on the high-efficiency solid-liquid separation effect of a membrane component, and is concerned in the field of sewage treatment. In order to realize the effective retention of autotrophic-heterotrophic biological denitrification granular sludge functional bacteria, an upflow anaerobic granular sludge blanket (UASB) -MBR reactor device is designed, and a functional bacteria dual-retention system of granular sludge and membrane components is constructed.

The invention uses a self-designed UASB-MBR reactor device to realize the rapid culture and formation of the autotrophic-heterotrophic organism denitrification granular sludge by optimizing and adjusting the operation parameters of a water inlet substrate, the dissolved oxygen content, the reflux control ascending flow rate and the like.

Disclosure of Invention

Aiming at the problems of the prior art, the device and the method for quickly culturing the autotrophic-heterotrophic organism denitrification granular sludge efficiently and quickly couple functional bacteria to form the autotrophic-heterotrophic organism denitrification granular sludge.

The invention provides the following technical scheme: a device for quickly culturing autotrophic-heterotrophic biological denitrification granular sludge comprises a water distribution tank, a high-level water tank, a UASB-MBR, a water production tank/water production backwashing tank and a chemical backwashing water tank, wherein the water distribution tank is connected with the high-level water tank through a pipeline provided with a high-level water tank lifting pump, a water outlet arranged at the bottom of the high-level water tank is connected with a water inlet arranged at the bottom of the UASB-MBR, an MBR membrane module is arranged at the middle part of the UASB-MBR, the upper end of the MBR membrane module is respectively connected with the water production tank/water production backwashing tank and the chemical backwashing tank through a pipeline provided with a water production/water production backwashing pump and a chemical backwashing pump, an internal circulation port a arranged at the upper end of the UASB-MBR is connected with an internal circulation port b at the bottom of the UASB-MBR through a pipeline provided with an internal circulation pump, a water area sleeve is arranged outside the UASB-MBR, the water area sleeve is connected with the water area circulating water tank through a pipeline provided with a water area circulating pump, and a heating rod is arranged in the water area circulating water tank.

Preferably, the high-level water tank lift pump continuously introduces the synthetic wastewater in the water distribution tank into the high-level water tank, the UASB-MBR reactor produces water through an MBR membrane component provided with a water production/water production backwash pump and a chemical backwash pump, and the synthetic wastewater in the high-level water tank is continuously supplemented into the UASB-MBR reactor.

Preferably, the UASB-MBR reactor has an effective volume of 4.5L.

Preferably, the number of the sampling ports is 3, the number of the sampling ports is 25cm at the front of the UASB-MBR reactor, a vertical pipe is arranged on the 3# sampling port, a pH/DO value probe is arranged in the vertical pipe, and the signal output ends of the pH/DO value probes are electrically connected with the signal input end of the WTW water quality analyzer.

Preferably, the bottom of the UASB-MBR is in an inverted cone shape, a water inlet, an aeration head, an internal circulation port b and a sludge taking port are arranged at the bottom of the UASB-MBR, and the aeration head is connected with an aeration pump through a pipeline provided with a gas flow meter.

Preferably, the effective membrane filament area of the MBR membrane component is 0.075 m²The material is PVDF and is positioned one third away from the top of the UASB-MBR reactor.

Preferably, a water production pressure gauge is arranged on the MBR membrane module and a water production pipeline of the water production/water production backwashing pump.

Preferably, the water production tank/water production backwashing tank is provided with a water inlet, a water production backwashing port and an overflow port, the water production/water production backwashing pump is a bidirectional pump and is connected with a water production pipeline and a water production backwashing pipeline, the water production direction is a forward direction, the water production backwashing direction is a reverse direction, the water production working condition is realized by opening a water production electromagnetic valve, closing the water production backwashing electromagnetic valve, the water production backwashing working condition is realized by opening the water production backwashing electromagnetic valve, and the water production electromagnetic valve is closed.

Preferably, the output ends of the high-level water tank lifting pump, the aeration pump, the water area circulating pump, the internal circulating pump, the chemical backwashing pump and the water production/water production backwashing pump are all in electric signal connection with the input end of the PLC control device, and the output end of the PLC control device is in electric signal connection with the input end of the PC computer.

A device for rapidly culturing autotrophic-heterotrophic organism denitrification granular sludge comprises the following culture methods of the autotrophic-heterotrophic organism denitrification granular sludge:

step one, inoculating 1000 ml of anaerobic ammonia oxidation flocculent sludge and 500 ml of anaerobic ammonia oxidation granular sludge in a UASB-MBR reactor, and then introducing NH₄ ⁺-N concentration 70 + -10 mg/L, NO₂ ^-Artificial synthetic wastewater with the N concentration of 90 +/-10 mg/L, controlling the temperature of the reactor to be 33 +/-2.0 ℃ in a water bath circulation manner, and controlling the rising flow rate to be 7.5 m/h; firstly, the hydraulic retention time is 24 h, and the water flux produced by the membrane is 2.5L/(m)²H) running for 20 d, then shortening the hydraulic retention time to 12 h, and ensuring that the water flux produced by the membrane is 5.0L/(m)²H) running for 20 d, then continuing to shorten the hydraulic retention time to 6.0 h, and the water flux produced by the membrane is 10L/(m)²H) running for 20 d, the final hydraulic retention time is 4.0h, and the water flux produced by the membrane is 15L/(m)²H) run 20 d;

step two, carrying out intermittent aeration in a UASB-MBR reactor, wherein the aeration stop ratio is 4.0: 10, aeration amount is 50 mL/min, dissolved oxygen concentration is controlled to be 0.2-0.5mg/L, water bath circulation control reactor temperature is 33 +/-2.0 ℃, rising flow rate is 8.0 m/h, hydraulic retention time is 6.0 h, and membrane water production flux is 10L/(m/m)²H); first with NH₄ ⁺N concentration of 200. + -.10 mg/L, NO₂ ^-Running the artificial synthetic wastewater with the N concentration of 100 +/-10 mg/L for 15 days; then with NH₄ ⁺N concentration of 200. + -.10 mg/L, NO₂ ^-The artificial synthetic wastewater with the N concentration kept at 60 +/-10 mg/L runs for 15 days; then with NH₄ ⁺N concentration of 200. + -.10 mg/L, NO₂ ^-The artificial synthetic wastewater with the N concentration kept at 20 +/-5.0 mg/L runs for 15 days; obtaining AOB-ANAMMOX granular sludge;

step three, introducing an organic carbon source into the artificial synthetic wastewater, wherein the substrate concentrations of the artificial synthetic wastewater are respectively NH₄ ⁺N concentration of 200. + -.10 mg/L, NO₂ ^-The N concentration is kept at 20 +/-5.0 mg/L, the COD concentration is 200 +/-15 mg/L, and the UASB-MBR reactor of the reactor continues to carry out intermittent aeration with the aeration stop ratioIs 4.0: 10, aeration amount is 50 mL/min, dissolved oxygen concentration is controlled to be 0.2-0.5mg/L, water bath circulation control reactor temperature is 33 +/-2.0 ℃, rising flow rate is 8.5 m/h, hydraulic retention time is 6.0 h, and membrane water production flux is 10L/(m/m)²H) culturing for 37 d under the condition of the sludge, thereby obtaining autotrophic-heterotrophic organism denitrification granular sludge;

detecting the concentrations of nitrate nitrogen, nitrite nitrogen, ammonia nitrogen and COD in inlet water and outlet water every day in the culture process, and calculating the removal rate of each nitrogen-containing index; recording the transmembrane pressure difference of the produced water; along with the stage change, the particle size of the granular sludge is gradually increased, and the ascending flow velocity is gradually increased.

Preferably, the artificial synthetic wastewater contains NH as a main component₄Cl、NaNO₂、NaHCO₃、KH2PO₄、MgSO₄·7H₂O、CaCl₂·2H₂O、FeSO₄、C₂H₃NaO₂(ii) a NaHCO with different concentrations in different stages of ammonia nitrogen and nitrite concentration₃The concentration is 500mg/L, KH₂PO₄The concentration is 27.2 mg/L, CaCl₂·2H₂O concentration of 180 mg/L, MgSO₄·7H₂The concentration of O is 300 mg/L; trace element i (g/L): 1.25 KHCO₃，0.025 KH₂PO₄，0.3 CaCl₂·2H₂O，0.2 MgSO₄·7H₂O，0.00625 FeSO₄(ii) a Microelement II (g/L): 15 EDTA, 0.43 ZnSO₄·7H₂O，0.24 CoCl₂·6H₂O，0.99MnCl₂·4H₂O，0.25 CuSO₄·5H₂O，0.22 NaMoO₄·2H₂O，0.19 NiCl₂·6H₂O，0.21 NaSeO₄·10H₂O，0.014 H₃BO₄，0.05 NaWO₄·2H₂O; the dosage of the trace elements I and II is 1.0 mL/L.

Preferably, the organic carbon source is anhydrous sodium acetate.

Preferably, the water production/water production backwashing pump and the chemical backwashing pump take 10 min as a period, the water production/water production backwashing pump and the chemical backwashing pump alternately operate, the water production flux of the operation of the MBR membrane component is 2.5-15L/(m 2 h), the backwashing flux is 3.5-22.5L/(m 2 h), the solution in the chemical backwashing water tank is sodium hypochlorite solution, the solution concentration is 0.5%, the water production/water production backwashing pump operates for 9.0 min under the water production working condition, then the water production/water production backwashing pump is stopped, the chemical backwashing pump operates for 30 s, and the period is completed.

Compared with the prior art, the invention has the following advantages:

(1) the anaerobic ammonia oxidation granular sludge and the anaerobic ammonia oxidation flocculent sludge are combined to be used as inoculated sludge, the sludge inoculation amount is high, the center of the autotrophic-heterotrophic organism denitrification granular sludge mainly uses anaerobic ammonia oxidation bacteria as a main body, the problems of slow growth rate and long generation time of the anaerobic ammonia oxidation bacteria are solved, the ammonia oxidation bacteria and the denitrifying bacteria are cultured on the basis of the anaerobic ammonia oxidation bacteria, and finally, the three main functional bacteria are efficiently coupled to form the autotrophic-heterotrophic organism denitrification granular sludge.

(2) On the basis of the UASB reactor, the MBR hollow fiber membrane is used for producing water, so that the loss of flocculent functional bacteria in the culture process of the autotrophic-heterotrophic organism denitrification granular sludge is effectively solved, and the flocculent functional bacteria are well kept in the reactor.

(3) The culture process adopts step-by-step continuous culture, the temperature is kept at 33 +/-2 ℃, dissolved oxygen is controlled, the reactor adopts internal reflux, the ascending flow speed is controlled through hydraulic conditions, and the shearing force is increased, so that the autotrophic-heterotrophic organism denitrification granular sludge is quickly formed.

Drawings

FIG. 1 is a schematic structural view of a device for rapidly culturing autotrophic-heterotrophic organism denitrified granular sludge.

In the figure: 1. a water distribution tank; 2. a high-level water tank; 3. a UASB-MBR reactor; 4. a water area circulating water tank; 5. a produced water tank/produced water backwashing tank; 6. a chemical backwash tank; 7. a three-phase separator; 8. an MBR membrane module; 9. a water area sleeve; 10. a pH/DO probe; 11. a WTW water quality detector; 12. a high level water tank lift pump; 13. an aeration pump; 14. a gas flow meter; 15. a water area circulating pump; 16. an internal circulation pump; 17. a chemical backwash pump; 18. water production/water production backwash pump; 19. a water production backwashing electromagnetic valve; 20. a water production solenoid valve; 21. a water production pressure gauge; 22. a heating rod; 23. a PLC control device; 24. a PC computer; 25. sampling ports (1#, 2#, and 3 #); 26. a water inlet; 27. an aeration head; 28. internal circulation ports (a, b); 29. and a mud taking port.

FIG. 2 is a graph showing the substrate concentration and removal rate of inlet and outlet water in different steps of a method for rapidly culturing autotrophic-heterotrophic organism denitrified granular sludge.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Referring to fig. 1, the apparatus for rapidly culturing autotrophic-heterotrophic biological denitrification granular sludge comprises a water distribution tank 1, a head tank 2, a UASB-MBR reactor 3, a product water tank/product water backwash tank 5 and a chemical backwash water tank 6; the water distribution tank 1 is connected with the high-level water tank 2 through a pipeline provided with a high-level water tank lifting pump 12, and the bottom of the high-level water tank 2 is provided with a water outlet which is connected with a water inlet 26 arranged at the bottom of the UASB-MBR reactor 3; the middle part of the UASB-MBR reactor 3 is provided with an MBR membrane component 8, and the upper end of the MBR membrane component 8 is respectively connected with a water production tank/water production backwashing tank 5 and a chemical backwashing tank 6 through pipelines provided with a water production/water production backwashing pump 18 and a chemical backwashing pump 17; the upper end of the UASB-MBR reactor 3 is provided with an internal circulation port a28 which is connected with an internal circulation port b28 at the bottom of the UASB-MBR reactor 3 through a pipeline provided with an internal circulation pump 16; a water area sleeve 9 is arranged outside the UASB-MBR reactor 3, the water area sleeve 9 is connected with the water area circulating water tank 4 through a pipeline provided with a water area circulating pump 15, and a heating rod 22 is arranged in the water area circulating water tank 4.

The operation flow is that the high-level water tank lift pump 12 continuously leads the artificial synthetic wastewater in the water distribution tank 1 into the high-level water tank 2, the UASB-MBR reactor 3 produces water through an MBR membrane component 8 provided with a water production/water production backwashing pump 18 and a chemical backwashing pump 17, and the artificial synthetic wastewater in the high-level water tank 2 is continuously supplemented into the UASB-MBR reactor 3.

Wherein, the inverted cone at the bottom of the UASB-MBR 3 is 10cm in height and 8.0 cm in diameter; the middle column part is 90 cm high, 8.0 cm in diameter and 4.5L in effective volume; the upper diameter of the upper inverted circular truncated part is 20 cm, the lower diameter is 8.0 cm, the height is 10cm, and the inclination angle is 60 degrees.

Wherein, the sampling ports 25 are arranged one at the front of the UASB-MBR reactor 3 at intervals of 25cm, and 3 sampling ports are arranged in total for collecting samples with different reaction heights; a pipe is arranged on the sampling port 3#25, a pH/DO value probe 10 is arranged in the vertical pipe, the signal output end of the pH/DO value probe 10 is in electric signal connection with the signal input end of the WTW water quality analyzer 11, the change of pH and DO in the UASB-MBR 3 is monitored in real time through the WTW water quality analyzer 11, and a basis is provided for real-time regulation and control.

Wherein, the bottom of UASB-MBR reactor 3 is equipped with water inlet 26, aeration head 27, internal circulation mouth b28 and gets mud mouth 29, and will intake water, aeration, circulation and sample and concentrate on the back taper fill part of bottom, and aeration head 27 links to each other through the pipeline that is equipped with gas flowmeter 14 with aeration pump 13, provides oxygen for UASB-MBR reactor 3, and through the dissolved oxygen content in the intermittent aeration control reactor.

Wherein, MBR membrane module 8 is restrainted form membrane module, and upper portion is the part of catchmenting, and the part size of catchmenting: the diameter is 7 cm, and the height is 10 cm; the lower part is membrane silk, the length of the membrane silk is 15 cm, and the effective membrane silk area is 0.075 m²The material is PVDF and is positioned one third away from the top of the UASB-MBR reactor 3.

Wherein, a water production pressure gauge 21 is arranged on a water production pipeline of the MBR membrane module 8 and the water production/water production backwashing pump 18, the transmembrane pressure difference of the MBR membrane module 8 is represented by the water production pressure gauge 21, and when the transmembrane pressure difference reaches 50Kpa, the MBR membrane module 8 needs to be taken out from the reactor for offline backwashing.

Wherein, produce water case/produce water backwash case 5 and set up the water inlet, produce water backwash mouth and overflow mouth, produce water/produce water backwash pump 18 and be two-way pump, link to each other with product water pipeline and product water backwash pipeline to produce the water direction and be forward, produce water backwash direction and be the reversal, produce the water operating mode, open and produce water solenoid valve 20, close and produce water backwash solenoid valve 19, produce water backwash operating mode, open and produce water backwash solenoid valve 19, close and produce water solenoid valve 20.

Wherein, the output ends of the high-level water tank lifting pump 12, the aeration pump 13, the water area circulating pump 15, the internal circulating pump 16, the chemical backwashing pump 17 and the water production/water production backwashing pump 18 are all connected with the input end of the PLC control device 23 through electric signals, the output end of the PLC control device 23 is connected with the input end of the PC computer 24 through electric signals, and the automatic operation of the UASB-MBR reactor 3 is realized through the cooperation of the PLC control device 23 and the PC computer 24.

A device for rapidly culturing autotrophic-heterotrophic organism denitrification granular sludge comprises the following culture methods:

step one, inoculating 1000 ml of anaerobic ammonia oxidation flocculent sludge and 500 ml of anaerobic ammonia oxidation granular sludge in a UASB-MBR reactor, and then introducing NH₄ ⁺-N concentration 70 + -10 mg/L, NO₂ ^-Artificial synthetic wastewater with the N concentration of 90 +/-10 mg/L, controlling the temperature of the reactor to be 33 +/-2.0 ℃ in a water bath circulation manner, and controlling the rising flow rate to be 7.5 m/h; firstly, the hydraulic retention time is 24 h, and the water flux produced by the membrane is 2.5L/(m)²H) running for 20 d, then shortening the hydraulic retention time to 12 h, and ensuring that the water flux produced by the membrane is 5.0L/(m)²H) running for 20 d, then continuing to shorten the hydraulic retention time to 6.0 h, and the water flux produced by the membrane is 10L/(m)²H) running for 20 d, the final hydraulic retention time is 4.0h, and the water flux produced by the membrane is 15L/(m)²H) run 20 d;

step two, carrying out intermittent aeration in a UASB-MBR reactor, wherein the aeration stop ratio is 4.0: 10, aeration amount is 50 mL/min, dissolved oxygen concentration is controlled to be 0.2-0.5mg/L, water bath circulation is controlled to control the temperature of the reactor to be 33 +/-2.0 ℃, the rising flow rate is 8.0 m/h, and water is addedThe force retention time is 6.0 h, and the water flux produced by the membrane is 10L/(m)²H); first with NH₄ ⁺N concentration of 200. + -.10 mg/L, NO₂ ^-Running the artificial synthetic wastewater with the N concentration of 100 +/-10 mg/L for 15 days; then with NH₄ ⁺N concentration of 200. + -.10 mg/L, NO₂ ^-The artificial synthetic wastewater with the N concentration kept at 60 +/-10 mg/L runs for 15 days; then with NH₄ ⁺N concentration of 200. + -.10 mg/L, NO₂ ^-The artificial synthetic wastewater with the N concentration kept at 20 +/-5.0 mg/L runs for 15 days; obtaining AOB-ANAMMOX granular sludge;

step three, introducing an organic carbon source into the artificial synthetic wastewater, wherein the substrate concentrations of the artificial synthetic wastewater are respectively NH₄ ⁺N concentration of 200. + -.10 mg/L, NO₂ ^-Keeping the N concentration at 20 +/-5.0 mg/L, keeping the COD concentration at 200 +/-15 mg/L, continuously carrying out intermittent aeration on a UASB-MBR reactor, and keeping the aeration stop ratio at 4.0: 10, aeration amount is 50 mL/min, dissolved oxygen concentration is controlled to be 0.2-0.5mg/L, water bath circulation control reactor temperature is 33 +/-2.0 ℃, rising flow rate is 8.5 m/h, hydraulic retention time is 6.0 h, and membrane water production flux is 10L/(m/m)²H) culturing for 37 d under the condition of the sludge, thereby obtaining autotrophic-heterotrophic organism denitrification granular sludge;

detecting the concentrations of nitrate nitrogen, nitrite nitrogen, ammonia nitrogen and COD in inlet water and outlet water every day in the culture process, and calculating the removal rate of each nitrogen-containing index; recording the transmembrane pressure difference of the produced water; along with the stage change, the particle size of the granular sludge is gradually increased, and the ascending flow velocity is gradually increased.

Wherein the main component of the artificial synthetic wastewater contains NH₄Cl、NaNO₂、NaHCO₃、KH2PO₄、MgSO₄·7H₂O、CaCl₂·2H₂O、FeSO₄、C₂H₃NaO₂(ii) a NaHCO with different concentrations in different stages of ammonia nitrogen and nitrite concentration₃The concentration is 500mg/L, KH₂PO₄The concentration is 27.2 mg/L, CaCl₂·2H₂O concentration of 180 mg/L, MgSO₄·7H₂The concentration of O is 300 mg/L; trace element i (g/L): 1.25 KHCO₃，0.025 KH₂PO₄，0.3 CaCl₂·2H₂O，0.2 MgSO₄·7H₂O，0.00625 FeSO₄(ii) a Microelement II (g/L): 15 EDTA, 0.43 ZnSO₄·7H₂O，0.24 CoCl₂·6H₂O，0.99MnCl₂·4H₂O，0.25 CuSO₄·5H₂O，0.22 NaMoO₄·2H₂O，0.19 NiCl₂·6H₂O，0.21 NaSeO₄·10H₂O，0.014 H₃BO₄，0.05 NaWO₄·2H₂O; the dosage of the trace elements I and II is 1.0 mL/L.

Wherein the organic carbon source is anhydrous sodium acetate.

Wherein, the water production/water production backwashing pump 18 and the chemical backwashing pump 17 take 10 min as a period, the water production/water production backwashing pump 18 and the chemical backwashing pump 17 alternately operate, and the water production flux of the operation of the MBR membrane component 8 is 2.5-15L/(m)²H) backwash flux of 3.5-22.5L/(m)²H), the solution in the chemical backwashing water tank 6 is a sodium hypochlorite solution, the concentration of the solution is 0.5%, the water production/water production backwashing pump 18 operates for 9.0 min under the water production working condition, then the water production/water production backwashing pump 18 stops, the chemical backwashing pump 17 operates for 30 s, and one period is completed.

As shown in FIG. 2, in the first step 1-80 d, the ammonia nitrogen removal rate and the total nitrogen removal rate at the initial stage of inoculation are 29.1% and 22.4%, respectively, and the total nitrogen removal load is 0.019 kg/(m)³D) anaerobic ammonium oxidation bacteria inoculated in the new environment have lower activity. The activity of anaerobic ammonia oxidizing bacteria is enhanced by shortening the hydraulic retention time (gradually from 24 h to 4.0 h), increasing the total nitrogen load of the inlet water, and when the hydraulic retention time is shortened to 4.0h, the ammonia nitrogen removal rate and the total nitrogen removal rate are respectively 97.9 percent and 81.7 percent, and the total nitrogen removal load is 0.485 kg/(m & lt/m & gt)³D), indicating that the activity of the anammox bacteria is restored; step two 81-126 d, carrying out intermittent micro-aerobic aeration culture on the enriched ammonia oxidizing bacteria on the basis of the step one, wherein the final ammonia nitrogen removal rate and the total nitrogen removal rate are respectively 92.3 percent and 78.6 percent, and the total nitrogen removal load is 0.547 kg/(m m.³D), indicating that AOB and ANAMMOX bacteria are well coupled to form AOB-ANAMMOX granular sludge under the culture of step twoThe combination is used for high-efficiency denitrification. Step three 127-₃ ^-The concentration of N is continuously reduced to 5.3 mg/L, the ammonia nitrogen removal rate and the total nitrogen removal rate are respectively 91.7 percent and 86.4 percent, and the total nitrogen removal load is 0.593 kg/(m)³D), further increase of total nitrogen removal and total nitrogen load, indicating that the three populations AOB, amammox and DNB in the reactor combine with each other to promote denitrification, enhance denitrification performance, and couple to form autotrophic-heterotrophic biological denitrification granular sludge.

After the culture in the first step 80 d, the particle size of the anaerobic ammonia oxidation granular sludge is greatly increased compared with that during inoculation, and the anaerobic ammonia oxidation granular sludge in the reactor is mainly flocculent sludge as an auxiliary; after the culture in the second step, the particle size of the granular sludge is further increased, the center color of the granules is dark red, the edge color is yellowish brown, the ammonia oxidizing bacteria mainly grow on the surfaces of the sludge granules and in floc sludge, the anaerobic ammonia oxidizing bacteria are located inside the granular sludge, and the ammonia oxidizing bacteria attach to the surfaces of the granules while the anaerobic ammonia oxidizing bacteria self-polymerize, so that the particle size of the sludge is increased, and the AOB-ANAMMOX granular sludge is formed; after the culture in the third step, the surface color of the granular sludge is changed into light gray, the interior of the granules is still red, and the addition of the carbon source promotes the mass propagation of the heterotrophic denitrifying bacteria to be attached to the surface of the granular sludge and present light gray, so that the grain diameter is further increased, and finally the autotrophic-heterotrophic organism denitrification granular sludge is formed

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.