阅读说明：本技术 饲料添加剂及包含其的饲料 (Feed additive and feed containing same ) 是由 廖亭彰 于 2018-09-10 设计创作，主要内容包括：一种饲料添加剂及包含其的饲料,增强动物免疫力,提升疫苗免疫效果,得以对抗病原菌的感染以及肥胖症。本发明的饲料添加剂包括：一菌体萃取物,包含多个菌株内含物,其中该些菌株内含物包括一多肽,该多肽包括：一转位肽,其用于转位；以及一结合表位。(A feed additive and feed containing the same can enhance animal immunity, improve vaccine immunity effect, and resist infection of pathogenic bacteria and obesity. The feed additive of the invention comprises: a bacterial extract comprising a plurality of bacterial strain contents, wherein the bacterial strain contents comprise a polypeptide comprising: a translocation peptide for translocation; and a binding epitope.)

1. A feed additive comprising:

a bacterial extract comprising a plurality of bacterial strain contents, wherein the bacterial strain contents comprise a polypeptide comprising:

a translocation peptide for translocation; and

a binding epitope.

2. A feed additive according to claim 1 wherein the translocation peptide is located at the N-terminus of the polypeptide and the binding epitope is located at the C-terminus of the polypeptide.

3. The feed additive of claim 1, wherein the polypeptide further comprises: a linker polypeptide, said linker polypeptide being positioned between said translocation peptide and said binding site.

4. The feed additive of claim 3, wherein the linker polypeptide is an M cell-targeted polypeptide or an intestinal epithelium-targeted polypeptide.

5. The feed additive of claim 3, wherein the linker polypeptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 1 to 4.

6. The feed additive according to claim 1, wherein the translocation peptide is from pseudomonas aeruginosa exotoxin.

7. The feed additive of claim 1, wherein the translocation peptide comprises a pseudomonas exotoxin a fragment with only functional region III removed.

8. The feed additive of claim 7, wherein the translocation peptide comprises a fragment of Pseudomonas aeruginosa exotoxin A that excludes domains Ib and III.

9. The feed additive of claim 8, wherein the translocation peptide comprises an amino acid sequence as set forth in SEQ ID NO: 5, respectively.

10. The feed additive according to claim 1, wherein the binding epitope is a newcastle disease virus epitope, an avian influenza virus epitope, a myostatin protein binding epitope, or a porcine reproductive and respiratory syndrome virus epitope.

11. The feed additive according to claim 1, wherein the strain is an escherichia coli strain, a lactobacillus strain, or an enterococcus strain.

12. The feed additive of claim 1, further comprising:

0.1 to 1% by weight of the microbial extract;

67 to 87 weight percent talc; and

10.5 to 18 weight percent of ethylcellulose.

13. The feed additive of claim 1, further comprising: 1.5 to 15 weight percent of resistant starch.

14. A feed, comprising:

0.03 to 5 weight percent of the feed additive of any one of claims 1 to 13; and

95 to 99.97 percent by weight of a feed matrix.

Technical Field

The invention relates to a feed additive and a feed containing the feed additive, in particular to a feed additive which can enhance the immunity of animals, improve the immune effect of vaccines and resist the infection of pathogenic bacteria and obesity and a feed containing the feed additive.

Background

In the case of raising livestock, in order to make livestock grow more lean meat and less intramuscular fat, and sell the livestock at a good price, poor breeders will add clenbuterol to increase the lean meat percentage of the livestock. However, clenbuterol is a B sympathetic receptor agonist, and when people eat meat containing clenbuterol, adverse effects such as heartbeat acceleration can be caused, and particularly, the effect is more remarkable for patients with hypertension or heart diseases; so that the addition of clenbuterol as a feed additive is forbidden nowadays. Therefore, if a feed additive could be developed which could replace clenbuterol without the aforementioned adverse effects, it would significantly contribute to the livestock breeding.

Newcastle disease (commonly known as fowl plague) and avian influenza (abbreviated as avian influenza) are viral diseases with high infectivity and high pathogenicity in avian birds. Once the infection of the birds is diagnosed, a large amount of killing is needed to prevent the birds from subsequently infecting other areas, and thus, the serious economic loss is caused. Therefore, if a feed additive capable of improving the resistance of the birds to the fowl plague or the avian influenza can be developed, so as to improve the antibody of the birds to the fowl plague or the avian influenza while feeding the feed, the serious economic loss caused by the disease can be avoided.

In addition, the porcine reproductive and respiratory syndrome is a porcine reproductive and respiratory syndrome which is an infectious disease with high infectivity, high lethality rate and high contact, and has the characteristics of wide disease area, high transmission speed and the like. In addition, once the piglet is infected by the mixed infection of the classical swine fever virus, the infection rate of the piglet to the respiratory diseases is more obvious, and particularly, the death rate of the piglets can be almost as high as 80 percent under the condition of poor pig farm management. Therefore, once the pig farm has the blue-ear virus invasion and the hog cholera epidemic prevention work is sparse, the herding infection of respiratory diseases is easily caused, and the great economic loss is caused to the cultured objects. Therefore, if a feed additive capable of improving the immunity of pigs against the blue-ear disease and the swine fever is developed to feed sows and piglets and improve the antibody of the pigs against the blue-ear disease, the major economic loss caused by the viral infection can be avoided.

Disclosure of Invention

The invention mainly aims to provide a feed additive and a feed containing the same, which can enhance the immunity of animals, improve the immune effect of vaccines and resist the infection of pathogenic bacteria and obesity. The feed additive of the invention comprises: an inactivated bacterial extract comprising a plurality of bacterial strain contents, wherein the bacterial strain contents comprise a polypeptide comprising: a translocation peptide for translocation; and a binding epitope. Wherein the translocation peptide may be located at the N-terminus of the polypeptide and the binding epitope may be located at the C-terminus of the polypeptide; and vice versa.

In the feed additive of the present invention, the polypeptide may further optionally include: a linker polypeptide. Wherein the linker polypeptide may be located between the translocation peptide and the binding site; or a translocation peptide is located between the linker polypeptide and the binding site. However, the present invention is not limited thereto; the relative positions of the translocation peptide, binding epitope and linker polypeptide can be varied depending on the desired objective.

In the feed additive of the present invention, the polypeptide formulation may be an M cell-targeted polypeptide or an intestinal epithelium-targeted polypeptide. For example, the linking polypeptide can comprise an amino acid sequence selected from the group consisting of SEQ ID NOs: 1 to 4.

In the feed additive of the invention, the translocation peptide is mainly derived from pseudomonas aeruginosa exotoxin. In one embodiment of the invention, the translocation peptide may comprise a fragment of pseudomonas aeruginosa exotoxin a with only functional region III removed. In another embodiment of the invention, the translocation peptide may comprise a fragment of pseudomonas aeruginosa exotoxin a that excludes domains Ib and III. In yet another embodiment of the invention, the translocation peptide may comprise a fragment of pseudomonas aeruginosa exotoxin a that excludes domains II, Ib, and III. In a further embodiment of the invention, the translocation peptide may comprise a partial N-terminal amino acid sequence of a pseudomonas aeruginosa exotoxin a fragment, e.g., as set forth in SEQ ID NO: 5.

In the feed additives of the invention, the binding epitopes may be designed to combat specific diseases. For example, the binding epitope can be a Newcastle Disease (ND) virus epitope, an Avian Influenza (AI) virus epitope, a myostatin protein binding epitope, a Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) epitope, or a swine fever (CSFV) epitope.

In the feed additive of the present invention, the strain to be used is not particularly limited as long as it can express the polypeptide, and preferably, a strain that expresses the polypeptide in a large amount can be used in the feed additive of the present invention. For example, the strain may be an escherichia coli strain, a bacillus subtilis strain, a lactobacillus strain, or an enterococcus strain.

The feed additive of the present invention may further include talc, ethyl cellulose, resistant starch, and the like, in addition to the deactivated microbial cell extract containing the polypeptide extract. Wherein the content of the deactivated mycopeptide extract can be 0.1 to 1 weight percent, the content of the talcum powder can be 67 to 87 weight percent, and the content of the ethyl cellulose can be 10.5 to 18 weight percent; and resistant starch can be added in order to enhance the slow release effect, and the content of the resistant starch can be 1.5 to 15 weight percent.

The preparation method of the feed additive of the invention can comprise the following steps: culturing the strain as described above; lysing the cultured strain, isolating Inclusion bodies (Inclusion bodies), and optionally lysing the strain to obtain a polypeptide extract; the polypeptide extract is mixed with talcum powder and ethyl cellulose, and resistant starch and the like can be added to obtain the feed additive.

In addition, the present invention further provides a feed comprising: a feed additive and a feed matrix as described above. Wherein, the content of the feed additive can be 0.03 weight percent to 5 weight percent, and the content of the feed matrix can be 95 weight percent to 99.97 weight percent. Alternatively, the feed additive may be present in an amount of 0.05 to 1.5 weight percent and the feed base may be present in an amount of 98.5 to 99.95 weight percent. The feed substrate may be any one selected from the group consisting of feeds commonly used in the art, depending on the animal to be fed (e.g., chicken, pig, sheep, cow, human, etc.).

The feed additive of the invention is mixed with a feed substrate to obtain the feed of the invention. When the feed is provided for an animal, the injection titer of the vaccine can be effectively improved, the efficacy of the animal for resisting specific diseases (such as chicken newcastle disease, chicken avian influenza or pig obesity, pig reproduction and respiration syndrome, swine fever and the like) is enhanced, and the immunity and the productivity of the animal are further improved.

Drawings

FIGS. 1 and 2 are graphs showing the results of the immunological efficacy of the chicken receiving the feed containing the feed additive according to example 2 of the present invention against newcastle disease virus only;

FIG. 3 is a graph showing the results of the immune efficacy against avian influenza virus of the chickens fed with the feed containing the feed additive of example 3 of the present invention.

Detailed Description

The following embodiments of the present invention are described by way of examples, and other advantages and effects of the present invention will be readily apparent to those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and its several details are capable of modification in various other respects, all without departing from the spirit and scope of the present invention.