Seed germination soil jacking force monitoring technical equipment and use method thereof
阅读说明:本技术 一种种子萌发顶土力监测技术装备及其用法 (Seed germination soil jacking force monitoring technical equipment and use method thereof ) 是由 江绪文 李贺勤 于 2018-12-17 设计创作,主要内容包括:本发明涉及种子科学领域,特别是关于一种种子萌发顶土力监测技术装备及其用法。它包括水浴恒温器、置管底板、置管板、测力盖、测力盖固定架、安放底板、数据信息采集箱、传感器单元、发芽管、恒温循环水浴器、电脑(含软件),其中各传感器单元对应目标发芽管,传感器单元可更换探头并具有伸缩性;它通过监测技术装备调试、种子处理与置床、基础参数设定、多通道顶土力信号集成采集与处理、目标数据提取与分析等作业完成子叶留(出)土型种子萌发顶土力的监测。本发明可控制种子萌发关键环境因素,并实现子叶留(出)土型种子萌发顶土力信号实时监测、量化存储。本发明可广泛应用于种子生物学研究、种子质量管理、实现良种化和种子质量标准化等方面。(The invention relates to the field of seed science, in particular to a seed germination soil jacking force monitoring technical device and a use method thereof. The device comprises a water bath thermostat, a tube placing bottom plate, a tube placing plate, a force measuring cover fixing frame, a placing bottom plate, a data information acquisition box, sensor units, a germination pipe, a constant-temperature circulating water bath and a computer (including software), wherein each sensor unit corresponds to a target germination pipe, and the sensor units can replace probes and have elasticity; the method completes the monitoring of the germination and soil jacking force of cotyledon remaining (grown) soil type seeds through the operations of debugging monitoring technical equipment, seed treatment and bed setting, basic parameter setting, multi-channel soil jacking force signal integrated acquisition and processing, target data extraction and analysis and the like. The invention can control the key environmental factors of seed germination and realize the real-time monitoring and quantitative storage of the germination soil jacking force signals of the cotyledon soil remaining (yielding) type seeds. The invention can be widely applied to the aspects of seed biological research, seed quality management, improved variety realization, seed quality standardization and the like.)
1. A technical device for monitoring the soil jacking force of seed germination comprises a water bath thermostat (1), a pipe placing bottom plate (11), a pipe placing plate (13), a force measuring cover (19), a force measuring cover fixing frame (26), a placing bottom plate (33), a data information acquisition box (44), a sensor unit (51), a germination pipe (17), a constant-temperature circulating water bath (68) and a computer (77) (containing software);
the water bath thermostat (1) is provided with a thermostat front logo (2), a temperature adjusting knob (3), a thermostat switch (4), a water level observation port (5), a tube placing plate placing groove (6), an airflow hole (7), a thermostat water outlet (8), a thermostat water inlet (9) and a thermostat power plug (10);
the tube placing bottom plate (11) is provided with a tube placing bottom plate hole (12);
the tube placing plate (13) is provided with an identification angle (16), a tube placing hole (14) and a tube position number (15);
the force measuring cover (19) is provided with a force measuring cover handle (20), a force measuring cover dismounting opening (21), a force measuring cover top surface logo (22), an LED lamp switch (30), a force measuring cover power plug (23), a sensor numerical control line (32), a sensor mounting plate (36), a power line through hole (37), a sensor fixer (38), a sensor number (39), a force measuring cover bottom plate mounting hole (40), an LED lamp panel (41), an LED lamp (42) and a sensor probe (43);
the force measuring cover fixing frame (26) is provided with a force measuring cover lifting beam (29), a force measuring cover rotating shaft (31), a lifting screw (27), a fixing button (28), a frame angle (24) and a frame angle fixing hole (25);
the placing bottom plate (33) is provided with a placing bottom plate groove (34) and a bottom plate fixing hole (35);
the data information acquisition box (44) is provided with a liquid crystal operation screen (45), a data interface (46), a storage battery switch (47), an acquisition box switch (48), a radiator (49) and an acquisition box power plug (50);
the sensor unit (51) is provided with a sensor connecting rod (52), a connector (53), a spring column (54), a chuck (58), a chuck sleeve (57), a probe rod (59), a probe (60), a sensor connecting rod thread (61), a connector thread (62), a spring column thread (63), a spring column ring (64), a spring column extending hole (65), a spring (55), a chuck thread (66), a chuck sleeve thread (67) and a chuck sleeve anti-slip thread (56);
the germination pipe (17) is provided with a depth scale (18);
the constant-temperature circulating water bath (68) is provided with a water injection tank (69), a liquid crystal display screen (70), a circulating switch (71), a temperature adjusting switch (72), a power switch (73), a water outlet (74), a water inlet (75) and a circulating water bath power plug (76);
the method completes the germination and soil jacking force monitoring of cotyledon remaining (grown) soil type seeds through the operations of debugging monitoring technical equipment, seed treatment and bed setting, basic parameter setting, multi-channel soil jacking force signal integrated acquisition and processing, target data extraction and analysis and the like.
2. The technical equipment for monitoring the germination soil jacking force of seeds and the use method thereof as claimed in claim 1, wherein: the monitoring technical equipment debugging operation comprises that a frame angle fixing hole (25) on a frame angle (24) of a force measuring cover fixing frame (26) corresponds to and is fixed with a tube placing bottom plate hole (12) on a tube placing bottom plate (11); the water bath thermostat (1) is placed between the placing bottom plate (33) and the placing bottom plate groove (34), a thermostat water inlet (9) and a thermostat water outlet (8) are respectively connected with a water outlet (74) and a water inlet (75) on the constant-temperature circulating water bath (68) through leather hoses (or the thermostat water outlet (8) is closed), the thermostat water inlet (9) is filled with water, and the water level is observed through the water level observation port (5); opening a data information collection box (44), a collection box switch (48) and a storage battery switch (47); the sensor numerical control line (32) is connected with the force measuring cover (19) and the data information acquisition box (44), and the data line connects the data information acquisition box (44) with the computer (77) through the data interface (46); selecting the specifications of the probe (60) and the germination pipe (17) according to the top soil extension characteristics of the germination tissue organs of the cotyledon-reserved (out) soil type seeds and the like; and finally, checking the working state of the technical equipment, the data signal transmission condition and the like, and debugging and solving in time if problems exist.
3. The technical equipment for monitoring the germination soil jacking force of seeds and the use method thereof as claimed in claim 1, wherein: the seed treatment and bed setting operation comprises the steps of selecting different solid germination matrixes (soil, sand, glass sand and the like) according to detection requirements, determining the particle size, the water content and the like of the matrixes, performing pre-sowing treatment on seed batches, selecting healthy and plump seeds with uniform sizes, disinfecting the seeds for 10 minutes by using a 1% sodium hypochlorite solution, cleaning the disinfected seeds for 3 times by using deionized water, and wiping off floating water on the surfaces of the seeds for later use; the seed placing depth is controlled according to the depth scales (18) on the germination pipes (17), a certain number (such as 1 seed) of seeds are placed in each germination pipe (17), and the seed placing mode is consistent (such as consistent direction of seed holes); the thickness of the seed covering of the substrate is consistent, and the surface of the substrate is ensured to be smooth.
4. The technical equipment for monitoring the germination soil jacking force of seeds and the use method thereof as claimed in claim 1, wherein: the basic parameter setting operation comprises the steps of injecting water into the water bath thermostat (1) through the thermostat water outlet (8) and the thermostat water inlet (9), exhausting air through the airflow hole (7), observing the water injection height through the water level observation port (5), opening the thermostat switch (4) of the water bath thermostat (1), setting the temperature (room temperature to 60 ℃ with a heating function) by using the temperature adjusting knob (3), or externally connecting the thermostat water outlet (8) and the thermostat water inlet (9) with a constant temperature circulating water bath (68); turning on a power switch (73), a circulation switch (71) and a temperature adjusting switch (72) of the constant-temperature circulating water bath (68), and adjusting the temperature, the circulation time and the like of circulating water through a liquid crystal display screen (70); opening the force measuring cover (19) and an LED lamp switch (30) to adjust the light intensity; the number of the monitored samples and the range of the area to be detected, the monitoring time length, the measuring range of the biological force and other parameters are set through a liquid crystal operation screen (45) of a data information acquisition box (44) or system software of a computer (77).
5. The technical equipment for monitoring the germination soil jacking force of seeds and the use method thereof as claimed in claim 1, wherein: the multichannel soil jacking force signal integrated acquisition and processing operation comprises the steps that a pipe placing bottom plate (11) is placed at the bottom of a water bath thermostat (1), a pipe placing plate (13) is correctly placed on a pipe placing plate placing groove (6) according to an identification angle (16), and a germination pipe (17) is placed according to a pipe position number (15) of the pipe placing plate (13); the force measuring cover (19) is vertically erected or horizontally placed through a force measuring cover handle (20), a force measuring cover lifting beam (29) and a force measuring cover rotating shaft (31), the force measuring cover (19) is controlled to move up and down through a lifting screw (27), and a fixed button (28) is used for braking; monitoring and controlling system software in a liquid crystal operation screen (45) or a computer (77); the sensor probe (60) is vertically aligned with a seed germination soil jacking area, a certain distance (about 1 cm) is reserved between the cotyledon soil retention type seed probe (60) and the bed surface, a smaller distance is reserved between the cotyledon soil output type seed probe (60) and the bed surface until the cotyledon soil output type seed probe and the bed surface are in flexible contact (the sensor unit (51) can bear certain longitudinal compression), and after an initial numerical value is set to zero before monitoring, real-time monitoring and quantitative storage of a seed germination soil jacking force signal are carried out.
6. The technical equipment for monitoring the germination soil jacking force of seeds and the use method thereof as claimed in claim 1, wherein: the target data extraction and analysis operation comprises the steps of opening a target storage file (containing information of soil jacking force of different channels at different time points and the like), selecting proper evaluation indexes and models to analyze and evaluate seed vitality and the like according to different crop seeds, and storing and printing analysis results.
Technical Field
The invention relates to the field of seed science, in particular to a seed germination soil jacking force monitoring technical device and a use method thereof.
Background
Throughout the entire growth period of the seeds, from seed germination to generation of new generation seeds, there is a constant concomitant action of various biological forces. The biological material signals are collected and utilized, so that the germination and growth rules of the seeds can be better understood, and a basis is provided for the research of related mechanisms. In Zhoudeman (1984), when the seeds germinate, there is a force that pushes the sprouts upward, and this force is referred to as germination capacity. In addition, according to the introduction of relevant contents in books such as the principle and method for measuring seed vigor (the introduction of the face, etc., 2006), the handbook for measuring seed vigor edited and published by the international seed inspection society, the handbook for measuring seed vigor edited and published by AOSA, and the like, and in combination with the previous research results, all the biological forces for promoting the tissue organ enlargement and the elongation growth of seedlings in the seed germination are collectively called as the seed germination force. It includes 4 kinds of biological force types, such as swelling force, germination force and seedling morphogenetic force. Wherein, in the germination stage, the seeds grow by the elongation of tissues and organs (such as root system, bud axis and coleoptile) and finally break through the soil layer to complete the emergence of seedlings, and the form of the biological power breaking through the soil layer is called as the topsoil power, which is an important form of the germination power. Based on previous research reports and previous test results, the seeds with high activity in seed germination have strong soil jacking force and high emergence rate; the seeds with low vigor have weak top soil force and can not normally emerge and even rot in soil. Therefore, the vigor of the seeds can be evaluated by comparing the germination and soil jacking force indexes of the seeds.
Based on a biological force sensing technology, more work is carried out on the aspect of measuring and evaluating the biological force of the seeds, and particularly certain progress is made on the aspect of monitoring and evaluating the germination soil jacking force of the seeds, including 2 patent authorizations (a corn seed germination soil jacking force measuring method 2014107631438; a seed germination soil jacking force detecting method 2014107945793) and the like, related work is also recognized and paid attention by the same lines, but some key technical problems still exist in the technical popularization and application, and need to be solved: the existing equipment lacks control over key factors of a seed germination environment; secondly, different plant seeds germinate in different soil-bearing forms, and the germination soil-bearing forms mainly comprise two types: the first type is cotyledon soil-retention type plants represented by corn, wherein the soil-retention type plants complete the soil jacking force signal acquisition and subsequent analysis work (which is solved earlier) through the elongation of a mesocotyl, the emergence of coleoptile and the contact of a coleoptile tip and a sensor probe; the second type is a cotyledon soil-out type plant represented by cotton, cotyledons are pushed out of the soil surface through elongation and bending of an embryonic axis and an embryo, and the soil jacking force measurement of seed germination of the cotyledon soil-out type plant relates to a series of problems of large soil jacking surface, effective contact between a tissue organ + a solid matrix and a sensor probe and the like, and directly influences the monitoring implementation effect of the soil jacking force and the correct evaluation of related indexes. Aiming at the existing main problems, the control of environmental factors of seed germination is enhanced, such as: temperature, illumination and the like, continuously improve and develop a flexible contact sensor unit suitable for monitoring the germination soil jacking force of cotyledon remaining (yielding) soil type seeds, and are very important for upgrading seed germination soil jacking force monitoring technical equipment and developing matched technical popularization and application.
Disclosure of Invention
Aiming at the problems, the invention aims to provide a technical device for monitoring the germination soil jacking force of seeds and a use method thereof, wherein the technical device can control key environmental factors of seed germination and can monitor and quantitatively store the soil jacking force signals of the germination soil-left (or soil-out) seeds of the cotyledons in real time on the basis of previous work. The popularization and application of the new technical equipment and the matching technology play an important role in the aspects of seed biological research, seed quality management, improved seed production, seed quality standardization and the like, and meanwhile, a reference is provided for monitoring and evaluating the germination capacity of seeds in other forms.
In order to achieve the purpose, the invention provides a technical device for monitoring the germination soil jacking force of seeds and a use method thereof by combining the soil jacking extension characteristic of germination tissue organs of cotyledon soil-remaining seeds and earlier research work. The device comprises a water bath thermostat, a tube placing bottom plate, a tube placing plate, a force measuring cover fixing frame, a placing bottom plate, a data information collecting box, sensor units, a germinating tube, a constant-temperature circulating water bath and a computer (containing software).
The water bath thermostat is 80cm long, 80cm wide and 20cm high, and is provided with a thermostat front logo, a temperature adjusting knob, a thermostat switch, a water level observation port, a tube plate placing groove, an airflow hole, a thermostat water outlet, a thermostat water inlet and a thermostat power plug.
The tube placing bottom plate is provided with 100 tube placing bottom plate holes.
The tube placing plate is provided with 1 identification angle and 100 tube placing holes, and each tube placing hole is provided with a corresponding tube position number.
The force measuring cover is provided with a force measuring cover handle, a force measuring cover dismounting opening, a force measuring cover top surface logo, an LED lamp switch, a force measuring cover power plug, a sensor numerical control wire, a sensor mounting plate, a power wire perforation, a sensor fixer, a sensor serial number, a force measuring cover bottom plate mounting hole, an LED lamp plate, an LED lamp and a sensor probe.
The force measuring cover fixing frame is provided with a force measuring cover lifting beam, a force measuring cover rotating shaft, a lifting screw, a fixing button, 2 frame angles and 4 frame angle fixing holes.
The placing bottom plate is provided with a placing bottom plate groove and 4 bottom plate fixing holes.
The data information acquisition box is provided with a liquid crystal operation screen, a data interface, a storage battery switch, an acquisition box switch, a radiator and an acquisition box power plug.
The sensor unit comprises a sensor connecting rod, a connector, a spring column, a chuck sleeve, a probe rod, a probe, sensor connecting rod threads, connecting head threads, spring column threads, a spring column ring, a spring column extending hole, a spring, chuck threads, chuck sleeve threads and chuck sleeve anti-skid threads.
The germination tubes are provided with depth scales.
The constant-temperature circulating water bath device is provided with a water injection tank, a liquid crystal display screen, a circulating switch, a temperature adjusting switch, a power switch, a water outlet, a water inlet and a power plug of the circulating water bath device.
The seed germination soil jacking force monitoring technical equipment and the using method thereof are mainly used for completing monitoring of seed leaf remaining (out) soil type seed germination soil jacking force through operations of debugging of the monitoring technical equipment, seed treatment and bed setting, basic parameter setting, multi-channel soil jacking force signal integrated acquisition and processing, target data extraction and analysis and the like.
The invention has the beneficial effects that: on the basis of earlier stage work, the technical equipment for monitoring the germination soil jacking force of the seeds and the use method thereof are provided, wherein the technical equipment can control key environmental factors (such as temperature and illumination) of seed germination, and can monitor and quantitatively store soil jacking force signals of germination of the soil-left seed (or soil-left seed) on the cotyledon in real time. The method plays an important role in the aspects of seed biological research, seed quality management, improved seed production, seed quality standardization and the like, and provides reference for monitoring and evaluating the germination capacity of seeds in other forms.
The present invention will be described in detail below with reference to the accompanying drawings and examples.
Drawings
FIG. 1 is a schematic view of the water bath thermostat of the present invention.
FIG. 2 is a schematic view of the placement and structure of the tube-placing bottom plate of the present invention.
FIG. 3 is a schematic diagram of the tube placing plate placement and its structure of the present invention.
FIG. 4 is a schematic view of the force-measuring lid, force-measuring lid mount, and mounting base of the present invention.
FIG. 5 is a schematic view of the internal and bottom structures of the force measuring lid of the present invention.
FIG. 6 is a schematic view of a data information collection box according to the present invention.
Fig. 7 is a schematic structural diagram of a sensor unit according to the present invention.
FIG. 8 is a schematic view of the structure of the constant temperature circulating water bath and the computer according to the present invention.
FIG. 9 is a flowchart illustrating the operation of the present invention.
In the figure, a
Detailed Description
As shown in fig. 1 to 8, a seed germination soil jacking force monitoring technical device and a use method thereof comprise a
The front face of the
The
A force
The force measuring cover fixing frame 26 is provided with a force measuring cover lifting beam 29, a force measuring cover rotating shaft 31, a screw 27 and a fixing button 28 on the side surface for adjusting the lifting of the
Two sides of the placing bottom plate 33 are respectively provided with 2 bottom plate fixing holes 35 for fixing the force measuring cover fixing frame 26, and two sides are provided with placing bottom plate grooves 34 for placing the
The data
The
The
The constant-temperature circulating
The
The use method of the invention comprises the following steps:
as shown in figure 9, the soil-pushing force signal of the cotyledon-reserved (out) soil type seed germination is monitored in real time and quantitatively stored based on the biological force sensing technology, so that the monitoring and evaluation of the soil-pushing force index of the seed germination are realized. The invention relates to the operations of debugging monitoring technical equipment, seed treatment and bed setting, basic parameter setting, multi-channel soil jacking force signal integrated acquisition and processing, target data extraction and analysis and the like.
The monitoring technical equipment debugging operation comprises that a force measuring cover fixing frame 26, frame angle fixing holes 25 on frame angles 24 correspond to and are fixed with pipe placing
The seed treatment and bed setting operation comprises the steps of selecting different solid germination matrixes (soil, sand, glass sand and the like) according to detection requirements, determining the particle size, the water content and the like of the matrixes, performing pre-sowing treatment on seed batches, selecting healthy and plump seeds with uniform sizes, disinfecting the seeds for 10 minutes by using a 1% sodium hypochlorite solution, cleaning the disinfected seeds for 3 times by using deionized water, and wiping off floating water on the surfaces of the seeds for later use; the seed placing depth is controlled according to the
The basic parameter setting operation comprises the steps of injecting water into the
The multichannel soil jacking force signal integrated acquisition and processing operation comprises the steps of placing a tube placing
The target data extraction and analysis operation comprises the steps of opening a target storage file (containing information of soil jacking force of different channels at different time points and the like), selecting proper evaluation indexes and models to analyze and evaluate seed vitality and the like according to different crop seeds, and storing and printing analysis results.
In summary, the invention is based on the acting characteristics of the germination soil jacking force of the cotyledon soil-left (out) type seeds and the control of the key environmental factors of the seed germination, and is based on the biological force sensing technology, a seed germination soil jacking force monitoring technical device and a use method thereof can control the key environmental factors of the seed germination, and realize the real-time monitoring, quantitative storage and the like of the germination soil jacking force signals of the cotyledon soil-left (out) type seeds.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting, and although the present invention is described in detail with reference to the embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions implemented by the present invention without departing from the design spirit and scope of the present invention, which should be covered by the claims of the present invention.
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