阅读说明：本技术 一种猪增生性肠炎的综合控制方法及系统 (Comprehensive control method and system for porcine proliferative enteritis ) 是由 赵建东 卫陆梅 项朝荣 李静 王路 解松林 李春贤 李建功 卫一新 聂慧琳 孙冰 于 2019-12-10 设计创作，主要内容包括：本发明提供了一种猪增生性肠炎的综合控制方法及系统,所述方法执行以下步骤：获取若干猪群的样本,包括种猪群和商品猪群；对样本中的猪进行猪增生性肠炎抗体的检测,获取检测数据；根据检测数据,将若干样本分为净化场猪群、稳定场猪群和流行场猪群,其中稳定场猪群的抗体阳性率大于净化场的抗体阳性率,小于流行场猪群的抗体阳性率；针对净化场猪群制定第一防控方案,针对稳定场猪群制定第二防控方案,并且针对流行场猪群制定第三防控方案。根据本发明的方法,分别针对净化场猪群、稳定场猪群和流行场猪群制定不同的防控方案,便于采取针对性的防控措施,减少此类疾病的发生,大大地减少经济损失,保护生猪饲养业发展,促进农民增收。(The invention provides a comprehensive control method and a comprehensive control system for porcine proliferative enteritis, wherein the method comprises the following steps: obtaining samples of a plurality of swineries, including breeding swineries and commercial swineries; detecting porcine proliferative enteritis antibodies in the sample to obtain detection data; according to the detection data, dividing a plurality of samples into a pigsty in a clean farm, a pigsty in a stable farm and a pigsty in an epidemic farm, wherein the antibody positive rate of the pigsty in the stable farm is greater than that of the pigsty in the clean farm and is less than that of the pigsty in the epidemic farm; a first prevention and control scheme is formulated for a pig farm in a clean farm, a second prevention and control scheme is formulated for a pig farm in a stable farm, and a third prevention and control scheme is formulated for a pig farm in a popular farm. According to the method, different prevention and control schemes are respectively formulated for the pig herd in the clean farm, the pig herd in the stable farm and the pig herd in the epidemic farm, so that targeted prevention and control measures are conveniently taken, the occurrence of diseases is reduced, the economic loss is greatly reduced, the development of the live pig breeding industry is protected, and the income increase of farmers is promoted.)

1. The comprehensive control method for the porcine proliferative enteritis is characterized by comprising the following steps:

step 1: obtaining a plurality of pig shoal samples, wherein the pig samples comprise a breeding shoal and a commercial shoal;

step 2: detecting the porcine proliferative enteritis antibody in the sample to obtain detection data;

and step 3: dividing a plurality of samples into a clean-field swinery, a stable-field swinery and an epidemic-field swinery according to the detection data, wherein the antibody positive rate of the stable-field swinery is greater than that of the clean field, and the antibody positive rate of the stable-field swinery is less than that of the epidemic-field swinery;

and 4, step 4: a first prevention and control scheme is formulated for the clean farm herd, a second prevention and control scheme is formulated for the stable farm herd, and a third prevention and control scheme is formulated for the epidemic farm herd.

2. The method of claim 1, wherein in step 4, establishing a first prevention and control plan for the clean-spot herd comprises:

one or more of a provenance control technique, a wild boar contact control technique, an environmental control technique, a field rodent control technique, a personnel tool control technique, a disinfection technique, wherein:

the seed source control technique includes: carrying out sample detection on preselected boar groups one by one at a called place, taking negative antibodies as candidate pigs to be transported back, putting the candidate pigs in an isolated observation field to carry out isolated observation for a preset number of days after the candidate pigs are transported back, collecting excrement samples to carry out sample detection during the isolated period, directly carrying out fattening treatment on positive pigs, and transferring the negative pigs to a back-up field for feeding;

the wild boar contact control technology comprises the following steps: seamless enclosing walls or epidemic prevention ditches are built in the field area, the field area gate and each pigsty gate are closed, and the wild boars are prevented from contacting with the swinery in the field area;

the environment control technology comprises the steps of separating a clean channel from a sewage channel, and establishing sound lighting, ventilation, heat preservation, heat insulation, mosquito and fly prevention and sewage harmless treatment facilities.

3. The method of claim 1, wherein in step 4, developing a second prevention scheme for the stable farm herd comprises: a breeding herd purification technique and/or a commercial herd control technique, wherein:

the swine herd purification technology comprises the steps of carrying out isolation feeding on all swine with positive antibodies, collecting excrement samples during the isolation feeding period, monitoring by a PCR method, monitoring for 2 times at intervals of 1 month, eliminating the swine with positive antibodies once, using the medicament aile fresh dressing for 7 days after 2 times of negative animals are used, and returning to a large herd after the body surface is disinfected;

the commercial swinery control technology comprises the steps of feeding all commercial swinery samples in and out, and feeding the commercial swinery samples in stages and at different points to prevent cross contamination.

4. The method of claim 1, wherein in step 4, developing a third prevention program for the pandemic herd comprises: the breeding herd grouping breeding technology comprises the steps of strictly distinguishing breeding personnel and breeding tools, making a elimination plan, updating 30% of positive pigs every year, and immunizing commercial herds with proliferative enteritis according to an immunization program.

5. The method of claim 1, wherein in step 2, detecting porcine proliferative enteritis antibodies in the sample comprises: and (3) detecting the porcine proliferative enteritis antibody of each pig in the breeding pig group, sampling and detecting the commercial pig group according to a proportion, and covering pigs at different days of age.

6. The method of claim 1, wherein in step 2, detecting porcine proliferative enteritis antibodies in the sample comprises: detecting porcine proliferative enteritis antibodies using one or more of a serological method, a smear microscopy method, or a molecular biological fluorescent PCR method, wherein the serological method employs Boringer Vargham porcine proliferative enteritis ELISA detection reagents.

7. The method of claim 1, wherein in step 3, classifying a number of the samples into a clean-site swinery, a stable-site swinery, and a popular-site swinery based on the detection data comprises:

samples with negative antibody detection of breeding swinery and commercial pigs are purification fields; a sample with the positive rate of the breeding pig group lower than 5 percent and the positive rate of the commercial pig group lower than 10 percent is a stable field; and the sample with the swinery positive rate of more than 5 percent and the commercial swinery positive rate of more than 10 percent is a flow field.

8. The method of claim 1, wherein after said step 4, said method further comprises the steps of:

acquiring physiological information of a plurality of healthy pigs, and establishing a physical condition identification model corresponding to each age group of the pigs according to the physiological information;

acquiring physiological information of the pig suspected to suffer from the hyperplastic enteritis, and transmitting the physiological information to the physical condition recognition model; identifying whether the physical condition of the pig is abnormal or not according to the physiological information, and alarming to a worker when the physiological information is identified to be abnormal;

the physiological information of the pig comprises one or more of weight information, feeding information and age information of the pig.

9. The method of claim 1, wherein in the step 2, in the step of detecting the porcine proliferative enteritis antibody in the sample to obtain the detection data, the temperature and the pH value of the liquid to be detected in the detection process are adjusted to improve the accuracy of the detection data, wherein the specific steps are as follows:

step A1, determining the maximum activity survival rate of the porcine proliferative enteritis antibody;

wherein f is the OD value in the liquid to be detected at the current time, f₀Is the initial OD value in the liquid to be detected, namely the OD value, K, in the liquid to be detected before the detection of the porcine proliferative enteritis antibody_maxIn order to obtain the maximum growth rate by using the formula, T is the standing time length of the liquid to be detected, e is a natural constant, Ts is a preset standing time length, f_maxThe maximum OD value in the standard liquid to be detected;

step A2, determining the adjusting temperature of the liquid to be detected;

wherein Te_bzFor said regulation of temperature, Te_dqIs the current temperature of the liquid to be detected, Te_minIs a preset minimum temperature, Te, of the liquid to be detected_maxThe preset highest temperature of the liquid to be detected;

step A3, determining the adjusted PH value of the liquid to be detected;

wherein the pH is_bzFor said adjustment of pH, PH_dqIs the current pH value, PH, of the liquid to be detected_minIs a preset minimum pH value, PH, of the liquid to be detected_maxThe pH value is the preset maximum pH value of the liquid to be detected;

and A4, adjusting the temperature of the liquid to be detected to be the adjusting temperature in real time, and adjusting the PH value of the liquid to be detected to be the adjusting PH value.

10. An integrated control system for porcine proliferative enteritis, comprising:

the sample acquisition module is used for acquiring samples of a plurality of swinery groups, wherein the samples of the pigs comprise breeding swinery groups and commercial swinery groups;

the detection module is used for detecting the porcine proliferative enteritis antibody in the sample to obtain detection data;

the sample classification module is used for classifying a plurality of samples into a clean farm swinery, a stable farm swinery and an epidemic farm swinery according to the detection data, wherein the antibody positive rate of the stable farm swinery is greater than that of the clean farm, and the antibody positive rate of the stable farm swinery is less than that of the epidemic farm;

and the prevention and control scheme making module is used for making a first prevention and control scheme aiming at the pig herd in the purification farm, making a second prevention and control scheme aiming at the pig herd in the stabilization farm and making a third prevention and control scheme aiming at the pig herd in the epidemic farm.

Technical Field

The invention relates to the technical field of livestock breeding, in particular to a comprehensive control method and system for porcine proliferative enteritis.

Background

Porcine proliferative enteritis is a contact infectious disease caused by Lawsonia intracellularis and is characterized by high proliferation of colon and cecum near ileum and blind node. Clinically, four major types are presented: porcine adenomatosis, Crohn's disease, hyperplastic hemorrhagic enteritis, necrotic enteritis. The infection rate of the disease is high, and the infection rates of pigs at different ages are 10-100%. Although the mortality rate of the disease is not high, about 5 percent, the disease seriously affects the growth of pigs, influences the feed conversion rate and often causes serious economic loss.

The disease is reported by Biester and Schware for the first time abroad, is distributed in all main pig-raising countries in the world at present, and is a common pig disease which is increasingly emphasized in pig-raising areas of all countries in the world in recent years. Epidemiological investigations of pig farms in many countries of europe, asia and north america have shown that pig farms investigated 1/4 and 1/2 among them have severe ileitis. Serological investigations showed that: the pathogen is present in more than 90% of pig farms in most countries. Bance D et al, 1997, investigated 184 swine herds in the United states, with a seropositive swine herd for proliferative enteritis of 96.2%. Therefore, the disease is considered to be one of the key etiological agents of porcine intestinal disease syndrome, and the swine industry in the europe and the united states suffers from huge economic losses, which amount to $ 2000 million per year in the united states alone.

At present, China has no corresponding systemic control scheme and technology for the porcine proliferative enteritis, so that a comprehensive control method and system for the porcine proliferative enteritis are provided.

Disclosure of Invention

In order to solve the technical problems, the invention provides a comprehensive control method and a comprehensive control system for porcine proliferative enteritis, which are used for preventing and treating the porcine proliferative enteritis.

The embodiment of the invention provides a comprehensive control method for porcine proliferative enteritis, which comprises the following steps:

step 1: obtaining a plurality of pig shoal samples, wherein the pig samples comprise a breeding shoal and a commercial shoal;

step 2: detecting the porcine proliferative enteritis antibody in the sample to obtain detection data;

and step 3: dividing a plurality of samples into a clean-field swinery, a stable-field swinery and an epidemic-field swinery according to the detection data, wherein the antibody positive rate of the stable-field swinery is greater than that of the clean field, and the antibody positive rate of the stable-field swinery is less than that of the epidemic-field swinery;

and 4, step 4: a first prevention and control scheme is formulated for the clean farm herd, a second prevention and control scheme is formulated for the stable farm herd, and a third prevention and control scheme is formulated for the epidemic farm herd.

Further, in the step 4, the making of the first prevention and control scheme for the pig farm swinery comprises: one or more of a provenance control technique, a wild boar contact control technique, an environmental control technique, a field rodent control technique, a personnel tool control technique, a disinfection technique, wherein:

the seed source control technique includes: carrying out sample detection on preselected boar groups one by one at a called place, taking negative antibodies as candidate pigs to be transported back, putting the candidate pigs in an isolated observation field to carry out isolated observation for a preset number of days after the candidate pigs are transported back, collecting excrement samples to carry out sample detection during the isolated period, directly carrying out fattening treatment on positive pigs, and transferring the negative pigs to a back-up field for feeding;

the wild boar contact control technology comprises the following steps: seamless enclosing walls or epidemic prevention ditches are built in the field area, the field area gate and each pigsty gate are closed, and the wild boars are prevented from contacting with the swinery in the field area;

the environment control technology comprises the steps of separating a clean channel from a sewage channel, and establishing sound lighting, ventilation, heat preservation, heat insulation, mosquito and fly prevention and sewage harmless treatment facilities.

Further, in the step 4, the making of the second prevention and control scheme for the stable pig farm comprises: a breeding herd purification technique and/or a commercial herd control technique, wherein:

the swine herd purification technology comprises the steps of carrying out isolation feeding on all swine with positive antibodies, collecting excrement samples during the isolation feeding period, monitoring by a PCR method, monitoring for 2 times at intervals of 1 month, eliminating the swine with positive antibodies once, using the medicament aile fresh dressing for 7 days after 2 times of negative animals are used, and returning to a large herd after the body surface is disinfected;

the commercial swinery control technology comprises the steps of feeding all commercial swinery samples in and out, and feeding the commercial swinery samples in stages and at different points to prevent cross contamination.

Further, in the step 4, the making of a third prevention and control plan for the popular farm swinery comprises: the breeding herd grouping breeding technology comprises the steps of strictly distinguishing breeding personnel and breeding tools, making a elimination plan, updating 30% of positive pigs every year, and immunizing commercial herds with proliferative enteritis according to an immunization program.

Further, in step 2, the detecting porcine proliferative enteritis antibodies in the sample comprises: and (3) detecting the porcine proliferative enteritis antibody of each pig in the breeding pig group, sampling and detecting the commercial pig group according to a proportion, and covering pigs at different days of age.

Further, in step 2, the detecting porcine proliferative enteritis antibodies in the sample comprises: detecting porcine proliferative enteritis antibodies using one or more of a serological method, a smear microscopy method, or a molecular biological fluorescent PCR method, wherein the serological method employs Boringer Vargham porcine proliferative enteritis ELISA detection reagents.

Further, in the step 3, dividing the plurality of samples into a clean-spot swinery, a stable-spot swinery and a popular-spot swinery according to the detection data includes:

samples with negative antibody detection of breeding swinery and commercial pigs are purification fields; a sample with the positive rate of the breeding pig group lower than 5 percent and the positive rate of the commercial pig group lower than 10 percent is a stable field; and the sample with the swinery positive rate of more than 5 percent and the commercial swinery positive rate of more than 10 percent is a flow field.

Further, after the step 4, the method further comprises the steps of:

acquiring physiological information of a plurality of healthy pigs, and establishing a physical condition identification model corresponding to each age group of the pigs according to the physiological information;

acquiring physiological information of the pig suspected to suffer from the hyperplastic enteritis, and transmitting the physiological information to the physical condition recognition model; identifying whether the physical condition of the pig is abnormal or not according to the physiological information, and alarming to a worker when the physiological information is identified to be abnormal;

the physiological information of the pig comprises one or more of weight information, feeding information and age information of the pig.

According to the comprehensive control method, different prevention and control schemes are respectively formulated for pig groups in a clean farm, stable farm and epidemic farm, so that targeted prevention and control measures can be conveniently taken, pig proliferative enteritis diseases of the pig groups can be discovered, prevented and treated early, the occurrence of the diseases can be reduced, economic loss can be greatly reduced, the development of the pig breeding industry can be protected, and income increase of farmers can be promoted.

Further, in the step 2, in the process of detecting the porcine proliferative enteritis antibody of the pig in the sample and acquiring the detection data, in order to improve the accuracy of the detection data, the temperature and the PH value of the liquid to be detected in the detection process need to be adjusted, wherein the specific steps are as follows:

step A1, determining the maximum activity survival rate of the porcine proliferative enteritis antibody;

wherein f is the OD value in the liquid to be detected at the current time, f₀Is the initial OD value in the liquid to be detected, namely the OD value, K, in the liquid to be detected before the detection of the porcine proliferative enteritis antibody_maxIn order to obtain the maximum growth rate by using the formula, T is the standing time length of the liquid to be detected, e is a natural constant, Ts is a preset standing time length, f_maxThe maximum OD value in the standard liquid to be detected;

step A2, determining the adjusting temperature of the liquid to be detected;

wherein Te_bzFor said regulation of temperature, Te_dqIs the current temperature of the liquid to be detected, Te_minIs a preset minimum temperature, Te, of the liquid to be detected_maxThe preset highest temperature of the liquid to be detected;

step A3, determining the adjusted PH value of the liquid to be detected;

wherein the pH is_bzFor said adjustment of pH, PH_dqIs the current pH value, PH, of the liquid to be detected_minIs a preset minimum pH value, PH, of the liquid to be detected_maxThe pH value is the preset maximum pH value of the liquid to be detected;

and A4, adjusting the temperature of the liquid to be detected to be the adjusting temperature in real time, and adjusting the PH value of the liquid to be detected to be the adjusting PH value.

The invention also provides a comprehensive control system for the porcine proliferative enteritis, which comprises the following components:

the sample acquisition module is used for acquiring samples of a plurality of swinery groups, wherein the samples of the pigs comprise breeding swinery groups and commercial swinery groups;

the detection module is used for detecting the porcine proliferative enteritis antibody in the sample to obtain detection data;

the sample classification module is used for classifying a plurality of samples into a clean farm swinery, a stable farm swinery and an epidemic farm swinery according to the detection data, wherein the antibody positive rate of the stable farm swinery is greater than that of the clean farm, and the antibody positive rate of the stable farm swinery is less than that of the epidemic farm;

and the prevention and control scheme making module is used for making a first prevention and control scheme aiming at the pig herd in the purification farm, making a second prevention and control scheme aiming at the pig herd in the stabilization farm and making a third prevention and control scheme aiming at the pig herd in the epidemic farm.

According to the comprehensive control system, the prevention and control scheme making module makes different prevention and control schemes for the pig herd in the clean farm, the stable farm and the popular farm respectively, so that targeted prevention and control measures can be conveniently taken, pig proliferative enteritis diseases of the pig herd can be discovered, prevented and treated early, the occurrence of the diseases can be reduced, economic loss can be greatly reduced, the development of the pig raising industry can be protected, and income increase of farmers can be promoted.

Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and claims hereof as well as the appended drawings.

The technical solution of the present invention is further described in detail by the accompanying drawings and embodiments.

Drawings

FIG. 1 is a schematic view of the comprehensive control method for porcine proliferative enteritis provided by the invention;

fig. 2 is a schematic diagram of a comprehensive control system for porcine proliferative enteritis provided by the invention.

Detailed Description

The preferred embodiments of the present invention will be described in conjunction with the accompanying drawings, and it will be understood that they are described herein for the purpose of illustration and explanation and not limitation.

The embodiment of the invention provides a comprehensive control method for porcine proliferative enteritis, which comprises the following steps as shown in figure 1:

step 1: obtaining a plurality of pig shoal samples, wherein the pig samples comprise a breeding shoal and a commercial shoal;

step 2: detecting the porcine proliferative enteritis antibody in the sample to obtain detection data;

and step 3: dividing a plurality of samples into a clean-field swinery, a stable-field swinery and an epidemic-field swinery according to the detection data, wherein the antibody positive rate of the stable-field swinery is greater than that of the clean field, and the antibody positive rate of the stable-field swinery is less than that of the epidemic-field swinery;

and 4, step 4: a first prevention and control scheme is formulated for the clean farm herd, a second prevention and control scheme is formulated for the stable farm herd, and a third prevention and control scheme is formulated for the epidemic farm herd.

The working principle of the method is as follows: after a sample of a swinery is obtained, detecting a porcine proliferative enteritis antibody of a pig in the sample to obtain detection data; dividing the sample into a pigsty in a clean farm, a pigsty in a stable farm and a pigsty in a popular farm according to the detection data; and then different prevention and control schemes are formulated for pig groups in clean farms, pig groups in stable farms and pig groups in epidemic farms.

The antibody positive rate of the purification field is smaller than that of the stable field swinery, and the antibody positive rate of the stable field swinery is smaller than that of the epidemic field swinery.

In step 2, the pig in the sample is subjected to detection of the pig proliferative enteritis antibody, and the detection comprises one or more of blood sample, feces sample and intestinal mucosa sample of the pig in the sample.

The method has the beneficial effects that: different prevention and control schemes are respectively formulated for pig groups in clean farms, stable farms and popular farms, so that targeted prevention and control measures can be conveniently taken, the pig proliferative enteritis diseases of the pig groups can be discovered, prevented and treated early, the occurrence of the diseases is reduced, the economic loss is greatly reduced, the development of the live pig breeding industry is protected, and the income increase of farmers is promoted.

In one embodiment, in the step 4, the establishing of the first prevention and control plan for the pig farm comprises:

one or more of a provenance control technique, a wild boar contact control technique, an environmental control technique, a field rodent control technique, a personnel tool control technique, a disinfection technique, wherein:

the seed source control technique includes: carrying out sample detection on preselected boar groups one by one at a called place, taking negative antibodies as candidate pigs to be transported back, putting the candidate pigs in an isolated observation field to carry out isolated observation for a preset number of days after the candidate pigs are transported back, collecting excrement samples to carry out sample detection during the isolated period, directly carrying out fattening treatment on positive pigs, and transferring the negative pigs to a back-up field for feeding;

the wild boar contact control technology comprises the following steps: seamless enclosing walls or epidemic prevention ditches are built in the field area, the field area gate and each pigsty gate are closed, and the wild boars are prevented from contacting with the swinery in the field area;

the environment control technology comprises the steps of separating a clean channel from a sewage channel, and establishing sound lighting, ventilation, heat preservation, heat insulation, mosquito and fly prevention and sewage harmless treatment facilities.

The working principle of the technical scheme is as follows: the provenance control technology uses colloidal gold test paper or a fluorescent PCR method to detect the fecal samples of preselected pigs one by one, and negative pigs can be transported back as candidate pigs. After being transported back, the manure is placed in an isolation observation field for isolation observation for 30 days, during the isolation period, a manure sample is collected for PCR detection, positive manure is directly fattened, and negative manure is transferred to a reserve field for feeding.

The wild boar contact control technology builds a seamless enclosing wall with the height of 2 meters in a field area or uses a closed type in an epidemic prevention ditch with the depth of 1.5 meters and the width of 2 meters, a field area gate and each pigsty gate, thereby preventing the wild boars from contacting with any contact in the field.

The disinfection technology is mainly divided into daily disinfection and terminal disinfection. Wherein, daily disinfection can set up four barriers in gate, production place, pig house entry and the pig house.

The beneficial effects of the above technical scheme are that: provides a prevention and control scheme aiming at the pig herd in the purification field.

In one embodiment, in the step 4, the making of the second prevention and control plan for the stable farm herd comprises: a breeding herd purification technique and/or a commercial herd control technique, wherein:

the swine herd purification technology comprises the steps of carrying out isolation feeding on all swine with positive antibodies, collecting excrement samples during the isolation feeding period, monitoring by a PCR method, monitoring for 2 times at intervals of 1 month, eliminating the swine with positive antibodies once, using the medicament aile fresh dressing for 7 days after 2 times of negative animals are used, and returning to a large herd after the body surface is disinfected;

the commercial swinery control technology comprises the steps of feeding all commercial swinery samples in and out, and feeding the commercial swinery samples in stages and at different points to prevent cross contamination.

The working principle of the technical scheme is as follows: the use of the drug aileron mix for 7 days includes the use of aileron mix at 500ppm for 7 days.

Commercial swinery is fed all in all out, cross contamination is prevented by feeding the commercial swinery in stages and in points, all in all out refers to that the pigs in the same batch enter a piggery (pig farm) at the same time, one piggery (pig farm) is discharged at the same time, and after all the pigs are taken out, the pigs are thoroughly cleaned and disinfected and then enter the next batch of pigs after being idle for a period of time, such as one week. Therefore, pathogens left by the pigs in the previous batch can be eliminated, a clean environment is provided for the newly-entered pigs, and the cyclic infection and the cross infection can be avoided.

The beneficial effects of the above technical scheme are that: provides a prevention and control scheme aiming at stable-field swinery.

In one embodiment, in the step 4, the making of a third prevention and control plan for the epidemic swinery includes: the breeding herd grouping breeding technology comprises the steps of strictly distinguishing breeding personnel and breeding tools, making a elimination plan, updating 30% of positive pigs every year, and immunizing commercial herds with proliferative enteritis according to an immunization program.

The working principle of the technical scheme is as follows: the commercial herd immunising against proliferative enteritis according to an immunisation programme comprises: the one-time immunization is carried out by using the proliferative enteritis attenuated live vaccine at the age of 30 days, or the booster immunization is carried out by using the proliferative enteritis attenuated inactivated vaccine at the age of 30 days for first immunization and at the age of 50 days for boosting immunization.

The beneficial effects of the above technical scheme are that: provides a prevention and control scheme aiming at the swinery in the epidemic farm.

In one embodiment, in step 2, the detecting porcine proliferative enteritis antibodies in the sample comprises: and (3) detecting the porcine proliferative enteritis antibody of each pig in the breeding pig group, sampling and detecting the commercial pig group according to a proportion, and covering pigs at different days of age.

The working principle of the technical scheme is as follows: sampling and inspecting the commercial swinery in proportion, and covering pigs at different days of age; 3-5% of commercial pigs are selected and inspected, and at least the herds with the ages of 0-30 days, 31-60 days, 60-90 days and more than 90 days are covered.

The beneficial effects of the above technical scheme are that: sample ranges for detecting porcine proliferative enteritis antibodies are provided.

In one embodiment, in step 2, the detecting porcine proliferative enteritis antibodies in the sample comprises: detecting porcine proliferative enteritis antibodies using one or more of a serological method, a smear microscopy method, or a molecular biological fluorescent PCR method, wherein the serological method employs Boringer Vargham porcine proliferative enteritis ELISA detection reagents.

The working principle of the technical scheme is as follows: pigs with ELISA value less than or equal to 0.50 are negative for porcine proliferative enteritis. Pigs with ELISA values greater than 0.50 were seropositive for porcine proliferative enteritis.

The beneficial effects of the above technical scheme are that: methods for detecting porcine proliferative enteritis antibodies in a pig in a sample are provided.

In one embodiment, in the step 3, the dividing the plurality of samples into a clean-spot swinery, a stable-spot swinery and a popular-spot swinery according to the detection data comprises:

samples with negative antibody detection of breeding swinery and commercial pigs are purification fields; a sample with the positive rate of the breeding pig group lower than 5 percent and the positive rate of the commercial pig group lower than 10 percent is a stable field; and the sample with the swinery positive rate of more than 5 percent and the commercial swinery positive rate of more than 10 percent is a flow field.

The beneficial effects of the above technical scheme are that: provides the classification basis of pig groups in clean farms, stable farms and popular farms.

In one embodiment, after the step 4, the method further comprises the steps of:

acquiring physiological information of a plurality of healthy pigs, and establishing a physical condition identification model corresponding to each age group of the pigs according to the physiological information;

acquiring physiological information of the pig suspected to suffer from the hyperplastic enteritis, and transmitting the physiological information to the physical condition recognition model; identifying whether the physical condition of the pig is abnormal or not according to the physiological information, and alarming to a worker when the physiological information is identified to be abnormal;

the physiological information of the pig comprises one or more of weight information, feeding information and age information of the pig.

The beneficial effects of the above technical scheme are that: according to the physiological information of the pigs, whether the physical conditions of the pigs are abnormal or not is identified, and when the physiological information is identified to be abnormal, an alarm is given to a worker to remind the worker to process in time.

The invention also provides a comprehensive control system for porcine proliferative enteritis, as shown in figure 2, comprising:

the sample acquisition module 201 is used for acquiring samples of a plurality of pig groups, wherein the samples of the pigs comprise breeding pig groups and commercial pig groups;

the detection module 202 is used for detecting the porcine proliferative enteritis antibody in the sample to obtain detection data;

a sample classification module 203, configured to classify a plurality of the samples into a clean farm swinery, a stable farm swinery and an epidemic farm swinery according to the detection data, where an antibody positive rate of the stable farm swinery is greater than that of the clean farm, and an antibody positive rate of the stable farm swinery is less than that of the epidemic farm;

and the prevention and control scheme making module 204 is used for making a first prevention and control scheme aiming at the pig herd in the purification farm, making a second prevention and control scheme aiming at the pig herd in the stabilization farm and making a third prevention and control scheme aiming at the pig herd in the epidemic farm.

The working principle of the technical scheme is as follows: the sample obtaining module 201 obtains samples of a plurality of swineries; the detection module 202 detects the porcine proliferative enteritis antibody in the sample to obtain detection data; the sample classification module 203 classifies a plurality of samples into a purification farm swinery, a stabilization farm swinery and a epidemic farm swinery according to the detection data; the prevention and control scheme making module 204 makes different prevention and control schemes for pig groups in clean farms, stable farms, and popular farms.

Wherein the antibody positive rate of the purification field is less than that of the stable field swinery, and the antibody positive rate of the stable field swinery is less than that of the epidemic field swinery.

The detection module 202 detects porcine proliferative enteritis antibodies in the pig in the sample, including one or more of blood samples, fecal samples, and intestinal mucosa samples of the pig in the sample.

The beneficial effects of the above technical scheme are that: the prevention and control scheme making module respectively makes different prevention and control schemes for pig groups in a clean farm, stable farm and popular farm, so that targeted prevention and control measures can be conveniently taken, pig proliferative enteritis diseases of the pig groups can be found, prevented and treated early, the occurrence of the diseases is reduced, economic loss is greatly reduced, the development of the live pig breeding industry is protected, and income increase of farmers is promoted.

In a specific embodiment, in the step 2, in the process of detecting the porcine proliferative enteritis antibody in the pig in the sample and acquiring the detection data, in order to improve the accuracy of the detection data, the temperature and the PH value of the liquid to be detected in the detection process need to be adjusted, wherein the specific steps are as follows:

step A1, determining the maximum activity survival rate of the porcine proliferative enteritis antibody;

wherein f is the OD value in the liquid to be detected at the current time, f₀Is the initial OD value in the liquid to be detected, namely the OD value, K, in the liquid to be detected before the detection of the porcine proliferative enteritis antibody_maxIn order to obtain the maximum growth rate by using the formula, T is the standing time length of the liquid to be detected, e is a natural constant, Ts is a preset standing time length, f_maxThe maximum OD value in the standard liquid to be detected;

step A2, determining the adjusting temperature of the liquid to be detected;

wherein Te_bzFor said regulation of temperature, Te_dqIs the current temperature of the liquid to be detected, Te_minIs a preset minimum temperature, Te, of the liquid to be detected_maxIs a preset maximum of the liquid to be detected(ii) temperature;

Te_minpreset value of (2) is 25 ℃, Te_maxThe preset value of (2) is 45 ℃.

Step A3, determining the adjusted PH value of the liquid to be detected;

wherein the pH is_bzFor said adjustment of pH, PH_dqIs the current pH value, PH, of the liquid to be detected_minIs a preset minimum pH value, PH, of the liquid to be detected_maxThe pH value is the preset maximum pH value of the liquid to be detected;

PH_minis set at a preset value of 6.5, pH_maxThe preset value of (2) is 8.2.

And A4, adjusting the temperature of the liquid to be detected to be the adjusting temperature in real time, and adjusting the PH value of the liquid to be detected to be the adjusting PH value.

Has the advantages that: by utilizing the technology, the PH value and the temperature of the liquid to be detected are adjusted before detection, so that the activity of the porcine proliferative enteritis antibody can be improved, the phenomenon that the activity of the porcine proliferative enteritis antibody is insufficient or the quantity of the porcine proliferative enteritis antibody is inaccurate due to environmental factors in the process of detecting the porcine proliferative enteritis antibody in a sample and acquiring detection data is avoided, and certain influence is caused on a detection result, so that the detection accuracy is improved.

Meanwhile, the temperature and the PH of the culture solution are controlled in real time by utilizing the technology, so that the best activity of the porcine proliferative enteritis antibody can be ensured in the process of culturing the porcine proliferative enteritis antibody in the culture solution.

It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.