阅读说明：本技术 春桑叶在制备抗衰老药物中的应用 (Application of spring mulberry leaf in preparing anti-aging medicine ) 是由 支德娟 赵成木 王欣 李洋 于 2019-12-18 设计创作，主要内容包括：本发明属于医药领域,具体涉及春桑叶在制备抗衰老药物中的应用。本发明发现春初采摘的春桑,能够显著提高早衰模型动物的寿命,延缓其衰老进程,能够被用于制备抗衰老药物和线粒体功能紊乱相关的疾病。(The invention belongs to the field of medicines, and particularly relates to application of spring mulberry leaves in preparation of an anti-aging medicine. According to the invention, the mulberry leaves picked in early spring can obviously prolong the service life of the premature senility model animal and delay the aging process, and can be used for preparing anti-aging medicaments and diseases related to mitochondrial dysfunction.)

1. The application of the spring mulberry leaves in preparing the anti-aging medicine is that the spring mulberry leaves are picked in the early spring.

2. The application of the mulberry leaf in spring according to claim 1, wherein the medicament is in the form of one of tablet, capsule, powder, syrup, granule, pill, mixture or drop pill.

3. The application of the spring mulberry leaves in the preparation of the medicament for delaying the premature senility is that the spring mulberry leaves are picked in the early spring.

4. The application of the mulberry leaf in spring as claimed in claim 3, wherein the dosage form of the medicine is one of tablet, capsule, powder, syrup, granule, pill, mixture or drop pill.

5. Application of spring mulberry leaves in preparation of medicines for treating diseases related to mitochondrial dysfunction is disclosed, wherein the spring mulberry leaves are mulberry leaves picked in the early spring.

6. The application of the mulberry leaf in spring as claimed in claim 5, wherein the dosage form of the medicine is one of tablet, capsule, powder, syrup, granule, pill, mixture or drop pill.

Technical Field

The invention belongs to the field of medicines, and particularly relates to application of spring mulberry leaves in preparation of an anti-aging medicine.

Background

Aging is a natural process of life, and with the increase of age, organisms have hypofunction, and the internal environment stability and the stress ability are reduced. Cells, tissues and organs in an aging body often show various degenerations in structure and function, and are accompanied by the generation of aging-related diseases such as diabetes, neurodegenerative diseases, cancer and the like, so that the quality of life is reduced, and a heavy burden is imposed on families and society (Collins et al, 2006; Muller et al, 2007).

The 21 st century is a century of aging of the population, which has brought about a series of very serious medical and social problems. In order to fundamentally improve the living quality of the elderly, a method for improving physical functions and delaying aging needs to be found to ensure that the elderly can be aged healthily. This goal has motivated modern scientists to screen for anti-aging drugs.

The application history of Chinese herbs in China has been 5000 years (Yu et al, 2010) so far. The traditional Chinese medicine also plays a role in improving the life quality of the old and promoting the healthy aging of the human. Folium Mori is derived from dried leaves of Morus albal. Generally, people divide mulberry leaves into spring mulberry, frost mulberry and mulberry leaves picked between the two. Spring mulberry is mostly used for eating, spring mulberry is mostly used for breeding silkworms after being picked, and frost mulberry is collected after being frosted for the first time and is used as a medicine. The frosted mulberry is obtained by removing impurities after picking and drying in the sun, is a wind-heat dispersing medicine and can be taken orally or applied externally. It is sweet and bitter in taste and cold in nature, enters lung and liver meridians, and has the functions of dispelling wind and heat, clearing lung-heat and moistening dryness, and clearing liver and improving vision. At present, effective components such as flavone, polyphenol, alkaloid, polysaccharide and the like are separated and extracted from downy mulberry, and the downy mulberry has various pharmacological activities such as oxidation resistance, inflammation resistance, blood sugar reduction, blood pressure reduction, blood fat reduction, immunity improvement, aging resistance, digestive system and excretory system health maintenance, tumor resistance and the like, and becomes a raw material for developing functional foods such as mulberry leaf tea, mulberry leaf juice beverage, mulberry leaf noodles, mulberry leaf powder and the like. Caenorhabditis elegans (Caenorhabditis elegans) has been used for life analysis abroad, and has been in progress for 30 years to date (Johnson and Wood, 1982). It is a good model organism for aging and anti-aging research. It is cheap and easy to culture; the generation period is short, the number of offspring is large, a large number of individuals with consistent backgrounds can be obtained, the experimental repeatability is ensured, and the experiment is carried out by adopting a large sample amount, so that the influence of individual difference is eliminated; conserved in gene and molecular pathways with higher organisms (Chua Seiko et al, 2008); nematodes live through the same aging process as humans, with progressive accumulation of lipofuscin in the gut, progressive loss of muscle motor capacity, and progressive loss of reproductive capacity until death (Vayndorf, 2009). Thus, nematodes became the first model organism for senescence studies (Johnson, 2008). Nematodes are also increasingly being used in the research and development of anti-aging traditional Chinese medicines in China (Chua Seijie et al, 2008; Konhongjie et al, 2011; Wang Xiaoyan et al, 2010; Kan et al; 2011).

The invention provides application of spring mulberry leaves in preparation of a medicine for resisting aging and treating diseases related to mitochondrial disorder. A large number of documents show that frosted mulberry leaves are the best to be used as medicines, although frosted mulberry leaves are frosted. Unexpectedly, compared with the mulberry leaves as frost, the mulberry leaves as picked in the beginning of spring have more obvious anti-aging effect, and are particularly suitable for preparing the medicament for delaying the premature senility.

Reference to the literature

Chua's outer jiao, Zhang Xinmin, Huangjianhua, Shenyi.2008. Experimental study of epimedium total flavonoids for delaying caenorhabditis elegans senescence. Chinese and Western medicine J28: 522-doped 525.

Korea Hongjie, Wangchang, Cheng reluctant, Li Fengjuan, Wang Yurong, Korea and Liangning 2011 initial research on the effect and mechanism of quercetin on nematode aging, amino acids and biological resources 33:35-38.

Wangxing, Wangxiang, Wangdan, Lilianda, Daihong, 2010, the effect of the Chuan Xiong extract on the life of nematodes and the study of the molecular mechanism, China Chinese materia medica 35:1042-1045.

Kaur, morning and morning, guo xi, gugui xiong 2011 influence of resveratrol on nematode longevity and reproduction by 60Co gamma irradiation 31: 162-.

Zhang Qiang, Sun Long wave, Zhang Jing, content determination of polysaccharide and total flavone in mulberry leaf, Jilin agriculture, 2011(3):73-74.

Johnson T.E.,Wood W.B.Genetic analysis of life-span in Caenorhabditiselegans.PNAS 79:6603-6607,1982.

Johnson T.E.,de Castro E.,Hegi de Castro S.,Cypser J.,Henderson S.,Tedesco P.Relationship between increased longevity and stress resistance asassessed through gerontogene mutations in Caenorhabditis elegans.Exp Gerontol36:1609-1617,2001.

Johnson T.E.Caenorhabditis elegans 2007:The premier model for thestudy of aging.Exp Gerontol 43:1-4,2008.

Muller F.L.,Lustgarten M.S.,Jang Y.,Richardson A.,Van Remmen H.Trendsin oxidative aging theories.Free Radic Biol Med 43: 477-503,2007.

Vayndorf E.Characterization of the antiaging effects of applephytochemicals using the C.elegans model.Theses and Dissertations. CornellUniversity.pp37,2009.

Yu Y.,Dosanjh L.,Lao L.,Tan M.,Shim B.S.,Luo Y.Cinnamomum cassia Barkin two herbal formulas increase life span in Carnohabditis elegans viainsulin signaling and stress response pathways.Plos One 5:e9339.doi:10.1371/journal.pone.0009339, 2010.

Disclosure of Invention

The invention aims to provide application of spring mulberry leaves in preparation of anti-aging medicaments.

The folium mori is picked in the early spring.

The invention also aims to provide application of the mulberry leaves in spring in preparing the medicine for delaying the premature senility, wherein the mulberry leaves are picked in the early spring.

The invention also aims to provide application of the mulberry leaves in preparing the medicines for treating diseases related to mitochondrial dysfunction, wherein the mulberry leaves are picked in the early spring.

The dosage form of the mulberry leaf spring of the invention is one of tablets, capsules, powder, syrup, granules, medicinal granules, pills, mixture or dropping pills.

The experiment is carried out by taking caenorhabditis elegans as model organisms, the caenorhabditis elegans is a mitochondrial respiratory chain complex II large subunit mev-1 mutant, a mitochondrial respiratory chain complex III iron-sulfur protein isp-1 mutant and an N2 wild type nematode respectively, and water extracts of different mulberry products are adopted to treat the tested caenorhabditis elegans. The results show that: the mulberry leaf water extract has a remarkable treatment effect on mitochondrial dysfunction, namely senilism caenorhabditis elegans, and tends to prolong the life of the nematode with mitochondrial dysfunction. However, the longevity of the N2 wild-type nematode was not affected. Meanwhile, the mulberry leaf water extract obviously improves the movement capacity of the senilism nematodes without influencing the reproduction of the senilism nematodes. The results indicate that the mulberry leaf water extract not only has the potential of delaying premature senility, but also has the potential of treating diseases related to mitochondrial dysfunction. Surprisingly, the anti-aging effect of the mulberry leaves in spring is more remarkable compared with the conventionally used frosted mulberry leaves.

The technical solution of the present invention is further described in detail with reference to the following specific examples, but the scope of the present invention is not limited to the following.

Drawings

FIG. 1 Effect of different aqueous extracts of folium Mori on the longevity of mev-1 senilising caenorhabditis elegans

FIG. 2 Effect of different aqueous extracts of folium Mori on longevity of isp-1 C.elegans

FIG. 3 Effect of different aqueous extracts of Mori folium on longevity of N2 normal wild type C.elegans

Detailed Description