阅读说明：本技术 一种含啶菌恶唑与氯啶菌酯的杀菌组合物及其应用 (Bactericidal composition containing pyridinconazole oxazole and nitrapyrin methyl and application thereof ) 是由 周明国 段亚冰 宋修仕 王建新 武健 于 2018-08-06 设计创作，主要内容包括：本发明涉及一种含啶菌恶唑与氯啶菌酯的杀菌组合物,其有效活性成分含有啶菌恶唑与氯啶菌酯,啶菌恶唑和氯啶菌酯的重量比为1-50:50-1,增效效果明显,可用于油菜菌核病、作物灰霉病、水稻纹枯病、小麦纹枯病、黄瓜白粉病、小麦白粉病的防治。(The invention relates to a bactericidal composition containing pyridinconazole and nitrapyrin methyl, the effective active ingredients of the bactericidal composition contain the pyridinconazole and the nitrapyrin methyl, the weight ratio of the pyridinconazole to the nitrapyrin methyl is 1-50:50-1, the synergistic effect is obvious, and the bactericidal composition can be used for preventing and treating sclerotinia rot of colza, gray mold of crops, rice sheath blight disease, wheat sheath blight disease, cucumber powdery mildew and wheat powdery mildew.)

1. The bactericidal composition containing the pyridinconazole and the nitrapyrin strobilurin is characterized in that the active ingredients are the pyridinconazole and the nitrapyrin strobilurin, the weight ratio of the pyridinconazole to the nitrapyrin strobilurin is 1-50:50-1, and the preferable weight ratio is 1-30: 30-1.

2. The bactericidal composition of claim 1, wherein the active ingredient is present in the composition in an amount ranging from 1% to 90%, preferably from 20% to 75%.

3. The bactericidal composition according to any one of claims 1 to 2, wherein the active ingredient is formulated into any formulation that is agriculturally acceptable with conventional adjuvants that are agriculturally acceptable in the formulation of the agricultural chemical.

4. The bactericidal composition of claim 3, wherein the formulation is water dispersible granules, wettable powder, suspoemulsion, aqueous emulsion, or microemulsion.

5. The use of the fungicidal compositions of claims 1-4 for the control of sclerotinia rot of colza, gray mold of crops, rice sheath blight, wheat sharp eyespot, cucumber powdery mildew, wheat powdery mildew.

Technical Field

The invention belongs to the field of agricultural bactericides, and particularly relates to a bactericidal composition containing pyridinconazole and nitrapyrin-methyl and application thereof.

Background

Pyrisoxazole, also known as: pyrisoxazole, chemical name: the 5- (4-chlorphenyl) -2, 3-dimethyl-3- (3-pyridyl) isoxazoline is an isoxazole heterocyclic substituted compound with a novel chemical structure, has high bactericidal activity, prevention and treatment effects, good systemic conductivity and good control effect on various plant diseases caused by ascomycetes, basidiomycetes and deuteromycetes. The structure formula of the pyrisoxazole is as follows:

the nitrapyrin-methyl is a novel methoxy acrylate (QoIs) bactericide, has the advantages of wide bactericidal spectrum, long persistent period and high bactericidal activity, is particularly excellent in the aspect of preventing and treating powdery mildew which is the most important crop disease, and is safe to crops and environment. In a bactericidal spectrum screening test, the nitrapyrin strobilus-B has good inhibitory activity on corn small spot disease, rice blast, false smut, rice sheath blight, wheat root rot, tomato gray mold, rape sclerotinia rot, peronophythora litchi and the like, and has good commercial development prospect and competitive advantage. The chemical structural formula is as follows:

practice shows that the long-term use of chemical agents easily causes the generation of drug resistance of pathogenic bacteria, the dosage is continuously increased, the risk is increased, and the sustainable development is not facilitated. Under the condition of lacking of disease-resistant varieties, in order to delay or overcome the formation and development of drug resistance of pathogenic bacteria, a single bactericide is prevented from being continuously used as much as possible, and the bactericide is used alternately or in a mixed manner with other types of bactericides, and pesticide compounding is one of the main ways for solving the occurrence of resistance. The pyridinconazole and the nitrapyrin are bactericides with proprietary intellectual property rights and developed by Shenyang chemical research institute, and the preliminary search of the applicant does not find a report that the pyridinconazole and the nitrapyrin are used in combination to control plant diseases.

The inventor compounds the pyridine bacterium oxazole and the nitrapyrin bacterium ester on the basis of indoor screening and field tests, finds that the pyridine bacterium oxazole and the nitrapyrin bacterium ester have obvious synergistic action on sclerotinia rot of colza, and completes the invention through further research.

Disclosure of Invention

The invention aims to solve the technical problem of providing a composition which has good bactericidal effect, low medication cost, wide bactericidal spectrum and obvious synergistic effect on sclerotinia rot of colza, gray mold of crops, rice sheath blight disease, wheat sharp eyespot, powdery mildew of cucumber and wheat powdery mildew. In order to solve the technical problems, the invention provides the following technical scheme:

the bactericidal composition containing the pyridinyloxy and the boscalid is characterized in that the active ingredients are the pyridinyloxy and the cloransulam-methyl, the weight ratio of the pyridinyloxy to the cloransulam-methyl is 1-50:50-1, the preferable weight ratio is 1-30:30-1, and in some preferable technical schemes: the weight ratio of the pyridinconazole to the nitrapyrin can be 1:50, 1:40, 1:30, 1:20, 1:10, 1:1, 10:1, 20:1, 30:1, 40:1, 50: 1; the content of effective components is 1-90%, preferably 20-75%.

The composition of the invention is mainly used for preventing and treating sclerotinia rot of colza, and if the sclerotinia rot of colza can be used for preventing and treating other plant fungal diseases, such as crop gray mold, rice sheath blight, wheat sharp eyespot, cucumber powdery mildew, wheat powdery mildew and the like.

The composition can be prepared into different preparation formulations according to a method known by a person skilled in the art, wherein the preparation formulations comprise water dispersible granules, wettable powder, suspending agents, aqueous emulsion, microemulsion and the like, and when the preparation formulations are prepared, known auxiliary agents such as emulsifying agents, wetting dispersing agents, suspending agents, disintegrating agents, binding agents, antifreezing agents, thickening agents, stabilizing agents, defoaming agents and other suitable auxiliary agents in pesticides can be added into the preparation formulations according to needs.

The advantages of the invention are obvious and mainly appear as follows:

1. the pyrisoxazole and the clodinafop-propargyl have no antagonistic action, and have obvious synergistic effect on sclerotinia sclerotiorum;

2. the composition of the invention expands the bactericidal spectrum and has better bactericidal effect; meanwhile, the application of the composition can delay or overcome the drug resistance of pathogenic bacteria and prolong the service life of the medicament.

3. The composition disclosed by the invention can reduce the use amount of each single agent by compounding and synergism, is low in cost and is easy to popularize.

Detailed Description

The present invention is further illustrated by the following examples, wherein the percentages are by weight, but the present invention is not limited thereto.

1. Indoor activity determination:

the indoor toxicity determination method comprises the following steps: preparing the pyridinconazole oxazole and the nitrapyrin strobilurin into required liquid medicine according to the set proportioning concentration for later use. The test method refers to part 2 of indoor bioassay test criteria bactericides of NY/T1156.2-2006 pesticide: the plate method of pathogenic fungus hypha growth inhibition test.

Under aseptic condition, quantitatively adding the pre-melted culture medium into an aseptic conical flask, sequentially and quantitatively sucking the liquid medicine from low concentration to high concentration, respectively adding into the above conical flasks, and sufficiently shaking. Then pouring the mixture into more than 3 culture dishes with the diameter of 9cm in equal amount to prepare the drug-containing plates with corresponding concentrations. Treatment without agent served as a blank. Repeat 4 times.

The cultured pathogenic bacteria are used for punching bacterial dishes from the edges of bacterial colonies by a puncher with the diameter of 5mm under the aseptic condition, the bacterial dishes are inoculated to the center of a drug-containing flat plate by an inoculation needle, the bacterial dishes are cultured for 2 days at the temperature of 25 ℃, the diameter of the bacterial colonies is measured by a cross method, and the effective medium dose (EC) for inhibiting the growth of the pathogenic bacteria by 50 percent by different treatments is calculated according to the following formula₅₀Value), and comparing the antibacterial activity, and evaluating the synergistic effect of the bactericidal composition containing the pyrisoxazole and the nitrapyrin-strobin on sclerotinia sclerotiorum by adopting a cotoxicity coefficient method. The joint action of the compound medicament on sclerotinia sclerotiorum is evaluated by the co-toxicity coefficient>120 is expressed as synergistic effect, the co-toxicity coefficient is expressed as additive effect between 80 and 120, and the co-toxicity coefficient<80 is shown as antagonistic.

TABLE 1 results of the combined action of compositions of different ratios on Sclerotinia sclerotiorum

Medicament	Proportioning	EC50(mg/L)	Co-toxicity coefficient
				Pyridinioxazole	——	0.192	——
Chloropyricarb ester	——	0.028	——
				Pyrisoxazole: chloropyricarb ester	1:50	0.022	129.44
Pyrisoxazole: chloropyricarb ester	10:30	0.028	127.15
				Pyrisoxazole: chloropyricarb ester	20:20	0.036	135.76
Pyrisoxazole: chloropyricarb ester	30:10	0.062	125.67
				Pyrisoxazole: chloropyricarb ester	50:1	0.132	130.47

The determination results in table 1 show that the composition of the pyrisoxazole and the nitrapyrin strobilurin has obvious synergistic effect on sclerotinia sclerotiorum within the ratio range of 1-50:50-1, and the drug effect is obviously better than the effect of each single-dose single test.

2. Formulation examples

The present invention is further illustrated by the following examples, wherein the percentages are by weight, but the present invention is not limited thereto, and the processing of the various dosage forms of the composition of the present invention is well known in the art and may vary depending on the circumstances.