阅读说明：本技术 一种血浆病毒灭活方法 (Plasma virus inactivation method ) 是由 吕茂民 武岳 朱凤宣 梁梦雅 孙珍珠 董秋瑛 屈洒洒 方迟 张梦君 李庆保 于 2019-08-20 设计创作，主要内容包括：本发明提供了一种血浆病毒灭活方法,包括向血浆中加入药用芦丁0.3-0.8g/L或芸香苷0.5-1.5mM作为灭活病毒的增强剂和血浆蛋白的保护剂,在一定强度UVC的辐照处理后能有效的灭活血浆中的有囊膜病毒和无囊膜病毒,解决了亚甲蓝光化学法不能灭活无囊膜病毒的问题,该方法还能减少血浆病毒灭活中蛋白质,特别是凝血因子的损失,有效保护了血浆中凝血因子的活性。(The invention provides a plasma virus inactivation method, which comprises the steps of adding 0.3-0.8g/L of medicinal rutin or 0.5-1.5mM of rutin into plasma as a virus inactivation reinforcing agent and a plasma protein protective agent, and effectively inactivating enveloped viruses and non-enveloped viruses in the plasma after irradiation treatment of certain strength UVC, thereby solving the problem that the non-enveloped viruses cannot be inactivated by a methylene blue photochemical method, reducing the loss of proteins, particularly blood coagulation factors, in plasma virus inactivation and effectively protecting the activity of the blood coagulation factors in the plasma.)

1. A method for inactivating virus in blood plasma comprises adding rutin 0.3-0.8g/L or rutin 0.5mM-1.5mM as virus inactivation synergist and plasma protein protectant into blood plasma; then, UVC light irradiation treatment is carried out.

2. A method for inactivating plasma viruses according to claim 1, wherein the rutin is a rutin injection or rutin.

3. The method for inactivating plasma viruses according to claim 2, wherein the pharmaceutical rutin is added in an amount of 0.3 to 0.8g, preferably 0.5g, by mass.

4. A method for virus inactivation of plasma according to claim 2, wherein rutin is added in an amount of 0.5-1.5mM, preferably 1.0 mM.

5. A method for the inactivation of plasma viruses according to claim 1, wherein the intensity of UVC light irradiation is: 500 to 5000. mu.w/cm²The light irradiation treatment time is as follows: and (3) 120-1200s, the light irradiation intensity and the irradiation time are mutually complementary, the irradiation treatment time is short when the light irradiation intensity is strong, and the irradiation time is prolonged when the light irradiation intensity is weak.

6. A method for the inactivation of a virus in plasma according to any of claims 1 to 5, wherein the UVC wavelength is in the range of 190 to 280nm, preferably 254 nm.

7. The method for inactivating plasma viruses according to claims 1 to 6, wherein the reduced titer of the indicator viruses in the plasma can reach not less than 4logs after the inactivation treatment.

8. Use of a method for the inactivation of a plasma virus according to any one of claims 1-7 for the preparation of a blood or plasma product.

9. The method for inactivating viruses in plasma according to any one of claims 1 to 8, wherein the plasma is derived from any source, including but not limited to human, porcine, canine, murine, bovine, equine, etc., and the collection route is derived from a living body after collecting blood or from an animal after sacrifice.

EXAMPLE 1 Primary reagents, raw materials and apparatus

The experimental plasma is fresh frozen dog plasma purchased from Beijing Tianqin I and Biotech limited;

pseudorabies virus and encephalomyocarditis virus were purchased from the Chinese veterinary microbial strain preservation management center;

PK-15 and BHK-21 cells were purchased from China veterinary microbial culture collection management center;

the medicinal rutin is produced by Sichuan synergetic pharmaceutical products GmbH;

rutin is produced by SIGMA/MERCK;

the plasma virus inactivation instrument is developed by Chinese medicine science and technology limited company;

the rest reagents and instruments are all conventional domestic models.