阅读说明：本技术 一种基于石墨烯纳米材料的植物萃取液等液体物质的低温灭菌方法和装置 (Low-temperature sterilization method and device for liquid substances such as plant extract liquid based on graphene nano material ) 是由 程金生 于 2018-08-08 设计创作，主要内容包括：本发明涉及一种基于石墨烯纳米材料的植物萃取液等液体物质的低温灭菌方法,包括以下步骤：使待灭菌液体流过石墨烯灭菌物,得到灭菌后的液体并储存；所述石墨烯灭菌物为氧化石墨烯、氟化石墨烯、氟化石墨烯氧化物以及石墨烯复合材料灭菌物中的一种或多种；所述石墨烯复合材料灭菌物为氧化石墨烯/银纳米颗粒复合材料、氧化石墨烯量子点/银纳米颗粒复合材料、氧化石墨烯/γ-Fe<Sub>2</Sub>O<Sub>3</Sub>纳米复合材料、氧化石墨烯量子点/Fe<Sub>3</Sub>O<Sub>4</Sub>复合材料、氧化石墨烯/氧化亚铜纳米复合材料、氧化锌/氧化石墨烯复合材料中的任意一种或多种。本发明通过使用具有灭菌功能的物质——石墨烯,使得常温下就可完成液体灭菌,从而避免了液体中的热不稳定物质被降解。(The invention relates to a low-temperature sterilization method for liquid substances such as plant extract liquid based on a graphene nano material, which comprises the following steps: enabling the liquid to be sterilized to flow through the graphene sterilization object to obtain sterilized liquidAnd storing the body; the graphene sterilization substance is one or more of graphene oxide, fluorinated graphene oxide and graphene composite sterilization substances; the graphene composite sterilization substance is a graphene oxide/silver nanoparticle composite material, a graphene oxide quantum dot/silver nanoparticle composite material, and graphene oxide/gamma-Fe 2 O 3 Nanocomposite and graphene oxide quantum dot/Fe 3 O 4 Any one or more of a composite material, a graphene oxide/cuprous oxide nanocomposite material and a zinc oxide/graphene oxide composite material. According to the invention, the graphene which is a substance with a sterilization function is used, so that liquid sterilization can be completed at normal temperature, and thus, the thermal unstable substances in the liquid are prevented from being degraded.)

1. A low-temperature sterilization method for liquid substances such as plant extract based on graphene nano materials is characterized by comprising the following steps:

enabling the liquid to be sterilized to flow through the graphene sterilization object to obtain sterilized liquid and storing the sterilized liquid;

the graphene sterilization substance is graphene oxide or fluorinated grapheneOne or more of fluorinated graphene oxide and graphene composite sterilization substances; the graphene composite sterilization substance is a graphene oxide/silver nanoparticle composite material, a graphene oxide quantum dot/silver nanoparticle composite material, and graphene oxide/gamma-Fe₂O₃Nanocomposite and graphene oxide quantum dot/Fe₃O₄Any one or more of a composite material, a graphene oxide/cuprous oxide nanocomposite material and a zinc oxide/graphene oxide composite material.

2. The method for low-temperature sterilization of liquid substances such as graphene nanomaterial-based plant extract according to claim 1, wherein the method comprises the following steps: the liquid to be sterilized is sterilized by ultrasonic wave with the frequency of 20KHz-95 KHz.

3. A low temperature sterilization device of liquid substances such as plant extract based on graphene nano-materials is characterized in that: comprises a pump-in valve, a low-temperature sterilization unit and a storage tank; the pump-in valve presses the liquid to be sterilized into the low-temperature sterilization unit; the low-temperature sterilization unit performs low-temperature sterilization on liquid to be sterilized to obtain sterilized liquid; the collector collects and stores the sterilized liquid; a graphene sterilizing object is arranged in the low-temperature sterilizing unit; the graphene sterilization substance is one or more of graphene oxide, fluorinated graphene oxide and graphene composite sterilization substances; the graphene composite sterilization substance is a graphene oxide/silver nanoparticle composite material, a graphene oxide quantum dot/silver nanoparticle composite material, and graphene oxide/gamma-Fe₂O₃Nanocomposite and graphene oxide quantum dot/Fe₃O₄Any one or more of a composite material, a graphene oxide/cuprous oxide nanocomposite material and a zinc oxide/graphene oxide composite material.

4. The device for low-temperature sterilization of liquid substances such as plant extract liquid based on graphene nano-materials according to claim 3, is characterized in that: the low temperature sterilization unit includes the post that disinfects, the inside sterilization screen cloth that is provided with a plurality of levels along the direction of liquid flow of post that disinfects, sterilization screen cloth surface coating the graphite alkene sterilization thing.

5. The device for low-temperature sterilization of liquid substances such as plant extract liquid based on graphene nano-materials according to claim 6, is characterized in that: a sterilization bag is further arranged inside the sterilization column and is positioned between the sterilization screens of adjacent levels; the graphene sterilization objects are wrapped on the sterilization bag, and meshes are arranged on the sterilization bag.

6. The device for low-temperature sterilization of liquid substances such as plant extract liquid based on graphene nano-materials according to claim 5, is characterized in that: the sterilization screen is a high-defect graphene oxide screen and/or a laser-induced graphene screen.

7. The device for low-temperature sterilization of liquid substances such as plant extract liquid based on graphene nano-materials according to claim 4, is characterized in that: the mesh number of the high-defect graphene oxide screen is sequentially increased along the flowing direction of the liquid, and the mesh number of the laser-induced graphene screen is sequentially increased along the flowing direction of the liquid.

8. The device for low-temperature sterilization of liquid substances such as plant extracts based on graphene nano-materials according to any one of claims 3 or 7, characterized in that: the low-temperature sterilization unit further comprises a sterilization tank located between the sterilization column and the sump; the inside graphite alkene sterilization thing of placing of sterilizing tank to be provided with supersonic generator.

9. The device for low-temperature sterilization of liquid substances such as plant extract liquid based on graphene nano-materials according to claim 8, wherein: the graphene sterilization substance in the sterilization bag is fluorinated graphene oxide; the graphene sterilizing material in the sterilizing tank is grapheneThe graphene composite sterilization substance is a graphene oxide/silver nanoparticle composite material, a graphene oxide quantum dot/silver nanoparticle composite material, and graphene oxide/gamma-Fe₂O₃Nanocomposite and graphene oxide quantum dot/Fe₃O₄One or more of a composite material, a graphene oxide/cuprous oxide nanocomposite material, and a zinc oxide/graphene oxide composite material; the ultrasonic generator emits ultrasonic waves with the frequency of 20KHz-95 KHz.

10. The device for low-temperature sterilization of liquid substances such as plant extracts based on graphene nano-materials according to any one of claims 8 or 9, characterized in that: the sterilizing column comprises a first sterilizing column and a second sterilizing column; the high-defect graphene oxide screens are arranged in the first sterilization column in a plurality of levels, the sterilization bag and the laser-induced graphene screens are arranged in the second sterilization column in a plurality of levels, and the sterilization bag and the laser-induced graphene screens are arranged in sequence along the flowing direction of liquid; valves are respectively arranged among the first sterilizing column, the second sterilizing column, the sterilizing pool and the storage tank.

Technical Field

The invention relates to the technical field of sterilization processes, in particular to a low-temperature sterilization method and device for liquid substances such as plant extract liquid based on graphene nano materials.

Background

In order to preserve these heat-unstable substances in the plant extract, various bacteria in the extract cannot be removed by adopting a high-temperature sterilization method, because the heat-unstable nutritive substances are decomposed while the microorganisms such as bacteria are removed at high temperature, the utilization value of the liquid substance is greatly reduced.

Therefore, it is necessary to develop a low-temperature sterilization method to effectively remove microorganisms such as bacteria in plant extracts and other liquids and simultaneously protect thermally unstable nutrients in the liquids from pyrolysis, so as to maximally retain nutritional or medicinal effective components in liquid substances such as plant extracts and improve the utilization value of the liquid substances.

Disclosure of Invention

In view of the above, an object of the present invention is to provide a method and an apparatus for low-temperature sterilization of liquid materials such as plant extracts based on graphene nanomaterials.

A low-temperature sterilization method for liquid substances such as plant extract based on graphene nano materials comprises the following steps:

enabling the liquid to be sterilized to flow through the graphene sterilization object to obtain sterilized liquid and storing the sterilized liquid;

the graphene sterilization substance is one or more of graphene oxide, fluorinated graphene oxide and graphene composite sterilization substances; the graphene composite sterilization substance is a graphene oxide/silver nanoparticle composite material or a graphene oxide quantum dot/silver nanoparticle composite materialGraphene oxide/gamma-Fe₂O₃Nanocomposite and graphene oxide quantum dot/Fe₃O₄Any one or more of a composite material, a graphene oxide/cuprous oxide nanocomposite material and a zinc oxide/graphene oxide composite material.

Compared with the prior art, the low-temperature sterilization method for liquid substances such as the plant extract based on the graphene nanomaterial utilizes the graphene, as the diameter of carbon atoms forming the graphene is very small and belongs to the nanometer level, and the diameter of bacteria is 0.5-5 microns, when the bacteria in the liquid substances such as the plant extract are contacted with the carbon atoms in the graphene, bacterial cells are cut by the carbon atoms with smaller diameters, so that the bacteria die, and the sterilization effect is achieved; therefore, bacteria can be killed at normal temperature without high temperature and high pressure, so that thermally unstable substances in the liquid to be sterilized are prevented from being degraded, and the plant extract to be sterilized is still rich in various nutrient substances after being sterilized. Meanwhile, the graphene oxide has a smaller diameter than graphene, and the surface of the graphene oxide is provided with a plurality of irregular columnar or needle-point-shaped protrusions, so that bacterial cells can be broken easily; the fluorinated graphene contains a fluorocarbon bond, the fluorocarbon bond can release a small amount of fluorine ions, the fluorine ions can inhibit the synthesis of polysaccharide and lipoteichoic acid, and the polysaccharide and the lipoteichoic acid are important components forming bacterial cell walls and cell membranes, so that the fluorine example can inhibit the multiplication of bacteria, and the higher the concentration of the fluorine ions is, the more the bacterial number is reduced; the fluorinated graphene oxide has the sterilization advantages of both fluorinated graphene and oxidized graphene. This application jointly uses the graphene sterilization material that after multiple improvement, have stronger bactericidal effect to disinfect, has effectively promoted bactericidal effect, obtains disinfecting more thorough liquid.

Further, the liquid to be sterilized is sterilized by ultrasonic wave with the frequency of 20KHz-95 KHz. The ultrasonic wave can destroy the cell structures of bacteria and parasitic ova, thereby playing the role of assisting the graphene sterilizing material in sterilizing and killing or blocking parasites.

The invention also provides a low temperature of liquid substances such as plant extract of the graphene nano materialA sterilization apparatus comprising a pump-in valve, a cryogenic sterilization unit, and a sump; the pump-in valve presses the liquid to be sterilized into the low-temperature sterilization unit; the low-temperature sterilization unit performs low-temperature sterilization on liquid to be sterilized to obtain sterilized liquid; the collector collects and stores the sterilized liquid; and a graphene sterilizing object is arranged in the low-temperature sterilizing unit. The graphene sterilization substance is one or more of graphene oxide, fluorinated graphene oxide and graphene composite sterilization substances; the graphene composite sterilization substance is a graphene oxide/silver nanoparticle composite material, a graphene oxide quantum dot/silver nanoparticle composite material, and graphene oxide/gamma-Fe₂O₃Nanocomposite and graphene oxide quantum dot/Fe₃O₄Any one or more of a composite material, a graphene oxide/cuprous oxide nanocomposite material and a zinc oxide/graphene oxide composite material.

Compared with the prior art, the low-temperature sterilization device for liquid substances such as plant extract based on graphene nano materials utilizes graphene, because the diameter of carbon atoms forming the graphene is very small and belongs to a nano level, and the diameter of bacteria is 0.5-5 microns, when the bacteria in the liquid substances such as the plant extract are contacted with the carbon atoms in the graphene, bacterial cells are cut by the carbon atoms with smaller diameters, so that the bacteria die, and the sterilization effect is achieved; therefore, bacteria can be killed at normal temperature without high temperature and high pressure, so that thermally unstable substances in the liquid to be sterilized are prevented from being degraded, and the plant extract to be sterilized is still rich in various nutrient substances after being sterilized. Meanwhile, the graphene oxide has a smaller diameter than graphene, and the surface of the graphene oxide is provided with a plurality of irregular columnar or needle-point-shaped protrusions, so that bacterial cells can be broken easily; the fluorinated graphene contains carbon-fluorine bonds capable of sterilization; the fluorinated graphene oxide has the advantages of both fluorinated graphene and oxidized graphene. Simultaneously, the graphene sterilization substance with various improved and stronger sterilization effects is used for sterilization, so that the sterilization effect is effectively improved, and more thorough liquid for sterilization is obtained. In addition, the low-temperature sterilization device only comprises the pumping valve, the low-temperature sterilization unit and the storage tank, and has the advantages of simple integral structure, easy manufacture and installation and easy popularization.

Further, the low temperature sterilization unit includes the sterilization post, the sterilization screen cloth of a plurality of levels is provided with along the direction of liquid flow inside the sterilization post, sterilization screen cloth surface coating the graphite alkene fungus thing that disinfects.

Further, a sterilization bag is arranged inside the sterilization column and is positioned between the sterilization screens of adjacent levels; the graphene sterilization objects are wrapped on the sterilization bag, and meshes are arranged on the sterilization bag.

Further, the sterilization screen is a high-defect graphene oxide screen and/or a laser-induced graphene screen.

Furthermore, the mesh number of the high-defect graphene oxide screen is sequentially increased along the flowing direction of the liquid, and the mesh number of the laser-induced graphene screen is sequentially increased along the flowing direction of the liquid.

Further, the low-temperature sterilization unit further comprises a sterilization tank located between the sterilization column and the sump; the graphene sterilizing material is placed in the sterilizing tank, and an ultrasonic generator is arranged in the sterilizing tank.

Further, the graphene sterilization substance in the sterilization bag is fluorinated graphene oxide; the graphene sterilizing material in the sterilizing tank is a graphene composite sterilizing material, and the graphene composite sterilizing material is a graphene oxide/silver nanoparticle composite material, a graphene oxide quantum dot/silver nanoparticle composite material, graphene oxide/gamma-Fe₂O₃Nanocomposite and graphene oxide quantum dot/Fe₃O₄One or more of a composite material, a graphene oxide/cuprous oxide nanocomposite material, and a zinc oxide/graphene oxide composite material; the ultrasonic generator emits ultrasonic waves with the frequency of 20KHz-95 KHz. The sterilization bag increases the sterilization strength by using the fluorinated graphene oxide which has the respective sterilization characteristics of fluoride and graphene oxide.

Further, the sterilization column comprises a first sterilization column and a second sterilization column; the high-defect graphene oxide screens are arranged in the first sterilization column in a plurality of levels, the sterilization bag and the laser-induced graphene screens are arranged in the second sterilization column in a plurality of levels, and the sterilization bag and the laser-induced graphene screens are arranged in sequence along the flowing direction of liquid; valves are respectively arranged among the first sterilizing column, the second sterilizing column, the sterilizing pool and the storage tank.

Drawings

Fig. 1 is a schematic structural diagram of a low-temperature sterilization device for liquid substances such as plant extract based on graphene nano-materials, and the like, according to the present invention;

FIG. 2 is a photograph of fluorinated graphene oxide used in the present invention, wherein FIG. 2a is a real image of fluorinated graphene oxide, and FIG. 2b is a transmission electron micrograph of fluorinated graphene oxide;

FIG. 3 is a flow chart of the low-temperature sterilization of the Camellia nitidissima extract from Fanghong in example II;

FIG. 4 is a GC-MS detection result diagram of various amino acids in the anti-hong Kong golden camellia extract liquid after the sterilization treatment of the low-temperature sterilization device of liquid substances such as the plant extract liquid based on the graphene nano material and the like;

FIG. 5 is a graph showing the GC-MS detection results of various amino acids in the extract of Camellia nitidissima from Turkey harbor after sterilization treatment by the conventional high-temperature sterilization technique; wherein, the high-temperature sterilization conditions are as follows: treating at 121 deg.C for 30 min.

Detailed Description

The principle of the low-temperature sterilization method of liquid substances such as plant extract liquid based on graphene nano materials is as follows: the graphene is a two-dimensional carbon nanomaterial, the diameter of carbon atoms forming the graphene is very small and is in a nanometer level, the diameter of bacteria is 0.5-5 micrometers, and after bacteria in liquid substances such as plant extract liquid and the like are contacted with the carbon atoms in the graphene, bacterial cells can be cut by the carbon atoms with the smaller diameter, so that the bacteria die, and the sterilization effect is achieved. Meanwhile, the ultrasonic wave with the frequency of 20KHz-95KHz has high energy quickly contained in vibration, and can disturb the normal metabolism of bacteria so as to play a sterilization role. The graphene sterilizing material and the ultrasonic wave can kill bacteria at normal temperature by utilizing the performance of the graphene sterilizing material and the ultrasonic wave without high temperature and high pressure, thereby preventing thermally unstable substances in the liquid to be sterilized from being damaged, and leading the plant extract liquid to be sterilized to be still rich in various nutrient substances after being sterilized.

Furthermore, the graphene oxide has a smaller diameter compared with graphene, and the surface of the graphene oxide is provided with a plurality of irregular columnar or needle-point-shaped protrusions, so that bacterial cells can be broken easily; the fluorinated graphene contains a fluorocarbon bond, the fluorocarbon bond can release a small amount of fluorine ions, the fluorine ions can inhibit the synthesis of polysaccharide and lipoteichoic acid, and the polysaccharide and the lipoteichoic acid are important components forming bacterial cell walls and cell membranes, so that the fluorine example can inhibit the multiplication of bacteria, and the higher the concentration of the fluorine ions is, the more the bacterial number is reduced; the fluorinated graphene oxide has the advantages of fluorinated graphene and oxidized graphene, and has stronger bactericidal capacity.

The invention discloses a low-temperature sterilization method for liquid substances such as plant extract based on graphene nano materials, which comprises the following steps of:

s1: enabling the liquid to be sterilized to flow through the graphene sterilization object to obtain sterilized liquid;

the graphene sterilization substance is one or more of graphene oxide, fluorinated graphene oxide and graphene composite material sterilization substances; the graphene composite sterilization substance is a graphene oxide/silver nanoparticle composite material, a graphene oxide quantum dot/silver nanoparticle composite material, and graphene oxide/gamma-Fe₂O₃Nanocomposite and graphene oxide quantum dot/Fe₃O₄Any one or more of a composite material, a graphene oxide/cuprous oxide nanocomposite material and a zinc oxide/graphene oxide composite material.

S2: storing the sterilized liquid;

and (5) introducing the sterilized liquid into a storage device for storage.

In the low-temperature sterilization method of liquid substances such as plant extract based on graphene nano materials, if the liquid to be sterilized is the extract, the extract needs to be obtained by a low-temperature extraction method; the low-temperature extraction method is any one of a supercritical fluid technology, a subcritical extraction technology, a molecular distillation technology and a low-temperature solute extraction technology.

The specific structure of the low-temperature sterilization apparatus for liquid substances such as a graphene nanomaterial-based plant extract according to the present invention will be described below. Please refer to fig. 1, which is a schematic structural diagram of a low-temperature sterilization apparatus for liquid substances such as plant extract based on graphene nano-materials according to the present invention. The low-temperature sterilization device for liquid substances such as plant extract based on graphene nano materials comprises a pumping valve 10, a low-temperature sterilization unit 20 and a collecting and storing device 30 in sequence from front to back. Wherein the pump-in valve 10 is used to press the liquid to be sterilized into the sterilization unit 20; the low-temperature sterilization unit 20 is used for performing low-temperature sterilization on the liquid to be sterilized to obtain sterilized liquid; the sump 30 is used to collect and store the sterilized liquid.

The low-temperature sterilization unit 20 comprises a first sterilization column 21, a second sterilization column 22 and a sterilization pool 23; and a first valve 24 is arranged between the first sterilizing column 21 and the second sterilizing column 22, a second valve 25 is arranged between the second sterilizing column 22 and the sterilizing pond 23, and a third valve 26 is arranged between the sterilizing pond 23 and the sump 30. The first valve 24, the second valve 25 and the third valve 26 are responsible for controlling the flow of the liquid sterilized in the previous part into the next part, so as to prevent the liquid from flowing into the next part due to insufficient sterilization in the previous part and prevent the liquid which enters the next part and is sterilized from flowing back to the previous part.

The first sterilizing column 21 is internally provided with a 3-15-level high-defect graphene oxide screen 211. The mesh number of the high-defect graphene oxide screen 211 is gradually increased along the liquid flowing direction, and the adding proportion of the high-defect graphene oxide in the high-defect graphene oxide screen is 5% -12.5%.

The interior of the second sterilization column 22 is filled with fluorinated graphene oxide 221, and a 3-8-level laser-induced graphene screen 222 is disposed at one end close to the sterilization tank 23. Please refer to fig. 2, which is a picture of the fluorinated graphene oxide used in the present invention, wherein fig. 2a is a picture of the fluorinated graphene oxide, and fig. 2b is a transmission electron microscope picture of the fluorinated graphene oxide. The fluorinated graphene oxide 221 is wrapped in 100-325 mesh regenerated fiber cloth or glass fiber cloth, and the fluorine doping proportion in the fluorinated graphene oxide 221 is 0.25% -1.25%. The mesh number of the graphene sterilization grids 222 increases along the flowing direction of the liquid.

The sterilizing tank 23 is filled with graphene sterilizing material 231 and is provided with an ultrasonic generator 232. The graphene sterilization object 231 is a graphene oxide/silver nanoparticle composite material, a graphene oxide quantum dot/silver nanoparticle composite material, and graphene oxide/gamma-Fe₂O₃Nanocomposite and graphene oxide quantum dot/Fe₃O₄Any one or more of a composite material, a graphene oxide/cuprous oxide nanocomposite material and a zinc oxide/graphene oxide composite material. The frequency of the ultrasonic wave generated by the ultrasonic wave generator 232 is 20KHz-95KHz, and the working time is 15-30 min.

The first sterilizing column, the second sterilizing column, the high-defect graphene oxide screen, the laser-induced graphene screen and the sterilizing tank are made of one of stainless steel, ceramic, glass, polyvinylidene fluoride, polytetrafluoroethylene and polyether ether ketone; the pump of the pump-in valve can be one of a peristaltic pump, a plunger pump or a diaphragm pump; the size of the laser-induced graphene screen is matched with the inner diameter of the second sterilization column, and the edge of the laser-induced graphene screen is provided with a protrusion device matched with the longitudinal groove on the inner wall of the second sterilization column, so that the laser-induced graphene screen is convenient to replace and clean; high defect oxidation graphite alkene screen cloth size matches with first sterilization post internal diameter, and its edge is provided with the vertical recess assorted projection means of first sterilization post inner wall, is convenient for change, washs. In addition, the high-defect graphene oxide screen can be prepared by directly forming a high-defect graphene oxide coating of 0.01-0.8 μm on a screen of a relevant size. The laser-induced graphene screen is prepared by forming a coating with the thickness of 0.01-0.8 mu m on a screen with a relevant size by adopting a laser-induced graphene nano material; in particular, laser-induced graphene nanomaterialsThe preparation method comprises the following steps: soaking a 6H-SiC silicon carbide wafer in acetone, removing organic residues on the surface by ultrasonic treatment for 15 minutes, and then irradiating a sample for 60 nanoseconds by using a xenon chloride excimer laser with the wavelength of 308nm, wherein the specific parameter of the xenon chloride excimer laser is that the single pulse energy density is 1.08J/cm²Frequency 5Hz, pulse number 60, defocus amount 3cm²The area of the light spot is 0.45cm²Mask area 8mm²And obtaining two layers of substances after laser irradiation, namely a polysilicon layer on the upper layer and a laser-induced graphene layer on the lower layer, then irradiating for 60 nanoseconds by using 193nm argon fluorine laser or 248nm krypton fluorine laser, wherein the frequency is 9Hz, the pulse number is 90, and laser irradiation is performed again to sublimate silicon, so that only the laser-induced graphene nano material is left.

The use method of the low-temperature sterilization device for liquid substances such as plant extract liquid based on the graphene nano material comprises the following steps: pumping the liquid to be sterilized into the first sterilizing column 21 by using the pump-in valve 10, and allowing the liquid to be sterilized to flow through the plurality of layers of high-defect graphene oxide screens 211 inside the first sterilizing column 21 in sequence for first sterilization to obtain a liquid for first sterilization; the liquid subjected to primary sterilization flows into the second sterilization column 22 through the first valve 24, and flows through the graphene fluoride oxide 221 and the 3-8-level graphene sterilization grid 222 in sequence for secondary sterilization to obtain liquid subjected to secondary sterilization; the liquid after the secondary sterilization flows into the sterilization tank 23 through the second valve 25, flows through the graphene sterilization object 231, and is subjected to ultrasonic sterilization at 20KHz-95KHz for 15-30min to obtain liquid after the tertiary sterilization; the liquid after the third sterilization is guided into the sump through the third valve 26 and the conduit, and meanwhile, the inert gas is introduced for sealing and storing.

The low-temperature sterilization apparatus for liquid substances such as plant extracts based on graphene nanomaterials of the present invention may have various modifications, for example, the sterilization screen may have a mesh structure such as a sterilization grid or a sterilization permeable membrane, as well as the shape of the screen; the sterilization screen can be not only a high-defect graphene oxide screen and a laser-induced graphene screen, but also a fluorinated graphene screen and the like; the number of the layers of the high-defect graphene oxide screen can be any, more than 3-15 layers; likewise, the number of layers of the laser-induced graphene screen can be any number of layers, more than 3-8 layers.