阅读说明：本技术 一种沙葱专用菌肥及其制备方法 (Special bacterial fertilizer for allium mongolicum regel and preparation method thereof ) 是由 姚拓 兰晓君 金艳丽 于 2019-09-16 设计创作，主要内容包括：本发明公开了一种沙葱专用菌肥及其制备方法,属于微生物技术领域。所述沙葱专用菌肥包括：苜蓿剑菌(Ensifer meliloti)GAU-00438和载体。本发明中通过该沙葱专用菌肥具有的固氮、解磷和分泌植物激素等能力,使得该沙葱专用菌肥可以在盐碱地使用,既可以减少化肥用量,提高土壤有机质含量,又可以改善了土壤结构,促进植物生长。(The invention discloses a special bacterial fertilizer for allium mongolicum regel and a preparation method thereof, and belongs to the technical field of microorganisms. The special bacterial fertilizer for allium mongolicum regel comprises: medicago sativa (Ensifer meliloti) GAU-00438 and a vector. According to the invention, the special bacterial fertilizer for allium mongolicum regel has the capabilities of fixing nitrogen, dissolving phosphorus, secreting phytohormone and the like, so that the special bacterial fertilizer for allium mongolicum regel can be used in saline-alkali soil, the using amount of a chemical fertilizer can be reduced, the organic matter content of the soil can be increased, the soil structure can be improved, and the plant growth can be promoted.)

1. The special bacterial fertilizer for allium mongolicum regel is characterized by comprising the following components in parts by weight: medicago sativa (Ensifer meliloti) GAU-00438 and a vector.

2. The special bacterial fertilizer for allium mongolicum regel of claim 1, wherein the carrier comprises: charcoal powder and humic acid.

3. The special bacterial fertilizer for allium mongolicum regel as claimed in claim 1, wherein the medicago sativum (Ensifermeliloti) GAU-00438 is preserved in the China center for type culture Collection in 2019 with the preservation number of CCTCC NO: m2019565.

4. A preparation method of a special bacterial fertilizer for allium mongolicum regel is characterized by comprising the following steps:

step (1), preparing a carrier: uniformly mixing charcoal powder and humic acid to obtain the carrier, and then sterilizing the carrier;

step (2), selecting strains: selecting the medicago sativa (Ensifer meliloti) GAU-00438;

step (3), preparing a strain culture solution: performing liquid culture on the medicago sativa (Ensifer meliloti) GAU-00438 to obtain a strain culture solution;

step (4), preparing the special bacterial fertilizer for allium mongolicum regel: and uniformly mixing the strain culture solution with the sterilized carrier, and performing standing culture to obtain the special bacterial fertilizer for allium mongolicum regel.

5. The method of claim 3, wherein step (1) comprises: mixing the charcoal powder and the humic acid according to the mass ratio of 1: 1 to obtain the carrier, wherein the pH value of the carrier after being uniformly mixed is 7.0 +/-0.2, and then the carrier after being uniformly mixed is subjected to high-pressure steam sterilization (103.4KPa, 121 ℃, 30 minutes) or gamma-ray sterilization treatment.

6. The method of claim 3, wherein the step (2) comprises: 17 plant rhizosphere growth-promoting bacteria are obtained from root systems of wild allium mongolicum regel, excellent phosphorus-solubilizing bacteria are screened by combining a PKO and Menkina culture medium with a molybdenum-antimony colorimetric resistance method, plant growth hormone (IAA) capacity is screened and quantitatively detected by a CCM-tryptophan culture medium and a method described by Goudjal, and 1 medicago sativa (Ensifer meliloti) GAU-00438 is selected.

7. The method of claim 3, wherein step (3) comprises: inoculating the medicago sativa (Ensifer meliloti) GAU-00438 into YMA culture medium, culturing at 28 deg.C and 180r/min for 48-72h until the content of viable growth-promoting bacteria in the culture solution reaches 1 × 10⁹cfu/ml, and obtaining a culture solution of the strain.

8. The method of claim 3, wherein the step (4) comprises: and uniformly mixing the strain culture solution and the sterilized carrier according to the volume mass ratio of 1:4, and then standing and culturing for 1 week at 28 ℃.

Technical Field

The invention relates to the technical field of microorganisms, and particularly relates to a special bacterial fertilizer for allium mongolicum regel and a preparation method thereof.

Background

The allium mongolicum regel, belonging to the genus allium of the family liliaceae of the angiosperma, is entitled Mongolian leek, wild allium mongolicum regel, shallot, cumuli and the like, and mainly grows on desert sand lands, grassland sand lands and arid sand slopes with the elevation of 800-2800 meters in inner Mongolia, Liaoning, Gansu, Xinjiang, Shanxi and the like in China. Researches show that the allium mongolicum regel contains more nutritional ingredients such as protein, fat, amino acid, vitamins and mineral substances, has extremely high nutritional value, contains active ingredients such as flavone, sulfide, volatile oil and polysaccharide, has special effects of reducing blood pressure, reducing blood fat, resisting tumors, resisting bacteria, diminishing inflammation, resisting oxidation, delaying aging, improving immunity of the organism and the like, and has excellent medicinal value. In addition, allium mongolicum regel is favored by various livestock, and parasitic infection of nasal cavities and eye cavities can be reduced after horses, sheep and camels eat the allium mongolicum regel; the sheep quickly and durably grips the pork when eating the allium mongolicum regel, and the pork does not smell the mutton and is not greasy; after horses are poisoned by eating crazyweed herbs, they can be grazed on grassland with allium mongolicum regel for detoxification. Therefore, the allium mongolicum regel is a green sandy herbaceous plant which has high nutritional value and can be used as both medicine and food, and is praised as 'good vegetables in sandy world' and 'lucid ganoderma in vegetables'. In addition, the role of allium mongolicum regel in ecology such as wind prevention, sand fixation, water and soil conservation and the like cannot be ignored.

Disclosure of Invention

In order to solve the problems in the prior art, the embodiment of the invention provides a special bacterial fertilizer for allium mongolicum regel and a preparation method thereof. The technical scheme is as follows:

on the one hand, the special bacterial fertilizer for allium mongolicum regel is provided, and comprises the following components: medicago sativa (Ensifermeliloti) GAU-00438 and a vector.

Further, the carrier includes: charcoal powder and humic acid.

Further, the medicago sativa (Ensifer meliloti) GAU-00438 is preserved in China center for type culture Collection in 2019, and the preservation number is CCTCC NO: m2019565.

On the other hand, the preparation method of the special bacterial fertilizer for allium mongolicum regel is provided, and comprises the following steps:

step (1), preparing a carrier: uniformly mixing charcoal powder and humic acid to obtain the carrier, and then sterilizing the carrier;

step (2), selecting strains: selecting the medicago sativa (Ensifer meliloti) GAU-00438;

step (3), preparing a strain culture solution: performing liquid culture on the medicago sativa (Ensifer meliloti) GAU-00438 to obtain a strain culture solution;

step (4), preparing the special bacterial fertilizer for allium mongolicum regel: and uniformly mixing the strain culture solution with the sterilized carrier, and performing standing culture to obtain the special bacterial fertilizer for allium mongolicum regel.

Further, the step (1) includes: mixing the charcoal powder and the humic acid according to the mass ratio of 1: 1 to obtain the carrier, wherein the pH value of the carrier after being uniformly mixed is 7.0 +/-0.2, and then the carrier after being uniformly mixed is subjected to high-pressure steam sterilization (103.4KPa, 121 ℃, 30 minutes) or gamma-ray sterilization treatment.

Further, the step (2) includes: 17 plant rhizosphere growth-promoting bacteria are obtained from root systems of wild allium mongolicum regel, excellent phosphorus-solubilizing bacteria are screened by combining a PKO and Menkina culture medium with a molybdenum-antimony colorimetric resistance method, plant growth hormone (IAA) capacity is screened and quantitatively detected by a CCM-tryptophan culture medium and a method described by Goudjal, and 1 medicago sativa (Ensifer meliloti) GAU-00438 is selected.

Further, the step (3) includes: inoculating the medicago sativa (Ensifer meliloti) GAU-00438 into YMA culture medium, culturing at 28 deg.C and 180r/min for 48-72h until the content of viable growth-promoting bacteria in the culture solution reaches 1 × 10⁹cfu/ml, and obtaining a culture solution of the strain.

Further, the step (4) includes: and uniformly mixing the strain culture solution and the sterilized carrier according to the volume mass ratio of 1:4, and then standing and culturing for 1 week at 28 ℃.

The technical scheme provided by the embodiment of the invention has the following beneficial effects: the special bacterial fertilizer for allium mongolicum regel has the advantages of low cost, safe use, good lasting effect, low non-renewable energy consumption, high economic benefit, no environmental pollution, no food pollution and the like; meanwhile, the special bacterial fertilizer for allium mongolicum regel can be used in saline-alkali soil to improve the content of organic matters in the soil, so that the using amount of the chemical fertilizer is reduced, the soil structure is improved, and the growth of plants is promoted.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.

FIG. 1 is a flow chart of a method for preparing a bacterial manure special for allium mongolicum regel provided by the embodiment of the invention;

fig. 2 is a flow chart of another method for preparing a special bacterial manure for allium mongolicum regel provided by the embodiment of the invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

Plant growth-promoting rhizobacteria (PGPR) refers to bacteria that live around, on, and in the root surface and root of a plant and secrete specific metabolites during its life cycle to promote the growth of the plant. Research shows that the bacteria generally have the capabilities of fixing nitrogen, dissolving phosphorus, secreting plant hormone, antibiotics and the like. In addition, the PGPR strain can also improve the soil structure, improve the content of organic matters in the soil, improve the saline-alkali soil and restore the capacity of the heavy metal polluted soil.

At present, research on allium mongolicum regel rhizosphere growth-promoting bacteria does not draw attention at home and abroad, so that allium mongolicum regel rhizosphere growth-promoting bacteria are screened, excellent strains with growth-promoting characteristics are obtained, and bacterial fertilizers prepared from the strain fertilizers are applied to artificial planting of allium mongolicum regel to improve the yield and the quality of the allium mongolicum regel, so that the allium mongolicum regel rhizosphere growth-promoting bacteria not only has positive significance for promoting the development of the planting industry of allium mongolicum regel, but also has.