阅读说明：本技术 一种源于沉香树叶片的生物发酵洗涤液的制备方法 (Preparation method of biological fermentation washing liquid derived from aquilaria sinensis leaves ) 是由 史学文 于 2019-11-20 设计创作，主要内容包括：本发明涉及微生物发酵技术,具体是指一种源于沉香树叶片的生物发酵洗涤液的制备方法,包括以下步骤：(1)筛选纤维素酶、蛋白酶高产菌株；(2)混合发酵；(3)粗滤,将发酵液过滤离心,得到澄清黄色浓稠液体；(4)精滤,向粗滤液中加入4％～5％的粉状活性炭,加热到50℃,充分搅拌2h后过滤,得到澄清液体；(5)制作洗涤剂。本发明充分地利用了农业废料,提高能量利用率,利用了沉香树叶片以及甘蔗渣的资源,变废为宝,生产出了十分良好的洗涤剂,实现了资源利用与避免环境污染的双丰收,除此之外,生物发酵洗涤液具有原料便宜、工艺环保以及排放少等优点,非常适合商业应用。(The invention relates to a microbial fermentation technology, in particular to a preparation method of biological fermentation washing liquid derived from aquilaria sinensis leaves, which comprises the following steps: (1) screening high-yield strains of cellulase and protease; (2) mixing and fermenting; (3) coarse filtering, filtering the fermentation liquor, and centrifuging to obtain clear yellow thick liquid; (4) fine filtering, adding 4-5% of powdered activated carbon into the coarse filtering liquid, heating to 50 ℃, fully stirring for 2h, and filtering to obtain clear liquid; (5) and (5) preparing a detergent. The invention fully utilizes agricultural waste, improves the energy utilization rate, utilizes the resources of the aquilaria sinensis leaves and bagasse, changes waste into valuable, produces a very good detergent, realizes the double harvest of resource utilization and environmental pollution avoidance, and in addition, the biological fermentation washing liquid has the advantages of cheap raw materials, environment-friendly process, less discharge and the like, and is very suitable for commercial application.)

1. A preparation method of biological fermentation washing liquid derived from aquilaria sinensis leaves is characterized by comprising the following steps: (1) screening high-yield strains of cellulase and protease; (2) mixing and fermenting; (3) coarse filtering, filtering the fermentation liquor, and centrifuging to obtain clear yellow thick liquid; (4) fine filtering, adding 4-5% of powdered activated carbon into the coarse filtering liquid, heating to 50 ℃, fully stirring for 2h, and filtering to obtain clear liquid; (5) and (5) preparing a detergent.

2. The method for preparing a bio-fermentation washing solution derived from leaves of an aquilaria sinensis tree according to claim 1, wherein the step (1) specifically comprises the following steps: A. collecting soil, namely collecting a soil sample in soil with rotten leaves under a bush, placing the soil sample in a test tube filled with 5mL of sterilized water, and vibrating and uniformly mixing;

B. coating a flat plate and culturing, taking 1mL of liquid in a test tube, sequentially diluting the liquid to 10-1, 10-2, 10-3, 10-4, 10-5, 10-6 and 10-7 in an equal gradient manner, taking 0.2mL of soil sample liquid with dilution times of 10-4, 10-5, 10-6 and 10-7, respectively adding the soil sample liquid to the casein flat plate and the CMC-Na flat plate for coating, then placing the soil sample liquid in an incubator at 37 ℃ for culturing for 48 hours, and observing the soil sample liquid;

C. selecting strains, wherein obvious transparent circles can appear around colonies on the surface of a casein plate after culture, dyeing a CMC-Na plate for 30min by using 0.2% congo red, then completely washing off a dye solution by using distilled water and 1mol/L NaCl in sequence, wherein the transparent circles can be seen around the colonies, respectively selecting 5 colonies with the diameters of the transparent circles larger than that of the colonies on the two plates, carrying out continuous streak culture until the colonies are pure, and inoculating the purified strains into a nutrient agar culture medium for storage and later use.

3. The method for preparing a bio-fermentation cleaning solution derived from aloeswood leaves as claimed in claim 1, wherein the step (2) comprises mixing 2-3 parts of bagasse, 2-3 parts of agilawood leaves and 7-10 parts of water by weight in a container, adding 0.1-0.2% by volume of slurry of cellulase with OD600 of 0.6-0.8, a protease high-yield strain culture solution and a bacillus megaterium culture solution into the container, and sealing. Fermenting at room temperature for 2 months.

4. The method according to claim 1, wherein the step (5) comprises, by weight, 3-5 parts of ferment filtrate, 18-22 parts of plant surfactant, and 1-4 parts of acid adjuvant; 0.1-0.5 part of caustic soda flakes; 0.3-0.5 part of chelating agent; 0.1-0.5 part of preservative; 1-1.5 parts of a thickening agent; 0.5-1 part of anti-redeposition agent; and mixing 67-74 parts of deionized water to prepare the detergent.

Technical Field

The invention relates to the technical field of microbial fermentation, in particular to a preparation method of biological fermentation washing liquid derived from aquilaria sinensis leaves.

Background

The enzyme is a high molecular substance having a biocatalytic function, and is an enzyme in nature, in a catalytic reaction system of the enzyme, a reactant molecule is called a substrate, and the substrate is converted into another molecule through catalysis of the enzyme. Almost all cellular processes require the involvement of enzymes to increase efficiency. Similar to other non-biological catalysts, enzymes accelerate the reaction rate by reducing the activation energy of the chemical reaction, and most enzymes can increase the rate of the reaction catalyzed by the enzymes by millions of times; likewise, the enzyme, acting as a catalyst, is not itself consumed during the reaction, nor does it affect the chemical equilibrium of the reaction. For example, proteases may catalyze the breakdown of proteins as biologically active catalysts. The lipase can be used as a bioactive catalyst to catalyze the decomposition of the lipase.

The enzyme detergent has the following advantages: (1) the enzyme can be added to help remove stains and oil from clothes by breaking down protein and fat. (2) The fermentation raw materials are usually rich in plant care essence, so that fabric fibers can be protected from being damaged while stains are removed, and the skin is not damaged. (3) The detergent is rich in specific dirt separation factors and bioactive enzymes, and can thoroughly decompose stubborn stains inside clothing fibers and on the surfaces of houses. (4) The fabric is rich in plant essence oil components, does not damage fabrics, effectively prevents the fabric of clothes from fading due to chemical reaction, and is softer after being washed. (5) The enzyme lotion has wider application range, can clean various machine washable clothes, and can wash objects on the surface, such as range hoods, gas stoves, wooden, ceramic tiles or marble floors, automobile bodies, water tanks, toilets, air conditioners and the like. (6) The enzyme lotion can effectively kill escherichia coli, staphylococcus and some harmful bacteria and can effectively inhibit bacterial spore propagation and generation after being diluted and dissolved and soaked for about twenty minutes. (7) The enzyme lotion has less foam, is easy to rinse, saves water, protects environment, and is convenient to clean articles which are not easy to be unpicked and washed, such as sofas, curtains and the like. (8) The enzyme lotion is mild and non-irritant, and is suitable for cleaning children clothes, underwear and the like.

The current detergents on the market generally contain phosphorus with a certain concentration, the phosphorus is a high-efficiency builder, and 15% of polyphosphate is added into washing powder, so that dirt in clothes can be effectively decomposed, and the clothes can be cleaned. However, after the washed sewage is discharged into rivers and lakes by taking phosphate as a main auxiliary agent, the phosphorus content in the water is increased, the water quality tends to eutrophication, various algae and aquatic weeds are bred in a large amount, the water quality is turbid, the water body is anoxic, and aquatic organisms such as fishes, shrimps and the like are dead. In addition, medical research shows that the phosphorus in the washing powder can directly influence the absorption of calcium by a human body when the washing powder with high phosphorus content is used for a long time, so that the human body is lack of calcium or the infantile osteomalacia is induced; when the high-phosphorus washing powder is used for washing clothes, the skin often has a burning feeling, namely, the high-phosphorus washing powder changes the acid-base environment in water to make the water more alkaline. If the laundry cannot be washed clean of residual phosphorus (which is difficult to do in practice because the phosphorus content can be reduced by washing the laundry for at least 5 minutes with running water), the residual phosphorus in the laundry can have irritating effects on the skin, especially on delicate skin of infants, after long-term accumulation. In addition, the strong alkaline phosphorus-containing washing powder is easy to damage the fabrics, especially the pure cotton, and the clothes can be burnt after the washing powder which achieves the decontamination purpose by strong alkaline is used for a long time.

In the process of making the old enzyme detergent, although the raw materials are changed into things of value by using fresh plant kitchen garbage, sugar is still put as one of the raw materials, and the making cost is increased. In addition, the old enzyme detergent is not prepared by adsorbing and decoloring fermented viscous liquid, and the fermented detergent is colored. In addition, the production of the old enzyme detergent uses natural bacteria for fermentation, and the quality of the protease of the strain is not high enough, so that the washing effect is influenced.

Disclosure of Invention

In order to solve the problems in the background art, the invention aims to provide a preparation method of a bio-fermentation cleaning solution derived from aquilaria sinensis leaves, which fully utilizes agricultural wastes, improves the energy utilization rate, utilizes the resources of the aquilaria sinensis leaves and bagasse, changes waste into valuable, produces a very good detergent, realizes the double harvest of resource utilization and environmental pollution avoidance, and in addition, the bio-fermentation cleaning solution has the advantages of cheap raw materials, environment-friendly process, less emission and the like, and is very suitable for commercial application.

In order to solve the technical problems, the technical scheme provided by the invention is as follows: a preparation method of biological fermentation washing liquid derived from aquilaria sinensis leaves comprises the following steps: (1) screening high-yield strains of cellulase and protease; (2) mixing and fermenting; (3) coarse filtering, filtering the fermentation liquor, and centrifuging to obtain clear yellow thick liquid; (4) fine filtering, adding 4-5% of powdered activated carbon into the coarse filtering liquid, heating to 50 ℃, fully stirring for 2h, and filtering to obtain clear liquid; (5) and (5) preparing a detergent.

Further, the step (1) specifically comprises the following steps: A. collecting soil, namely collecting a soil sample in soil with rotten leaves under a bush, placing the soil sample in a test tube filled with 5mL of sterilized water, and vibrating and uniformly mixing;

B. coating a flat plate and culturing, taking 1mL of liquid in a test tube, sequentially diluting the liquid to 10-1, 10-2, 10-3, 10-4, 10-5, 10-6 and 10-7 in an equal gradient manner, taking 0.2mL of soil sample liquid with dilution times of 10-4, 10-5, 10-6 and 10-7, respectively adding the soil sample liquid to the casein flat plate and the CMC-Na flat plate for coating, then placing the soil sample liquid in an incubator at 37 ℃ for culturing for 48 hours, and observing the soil sample liquid;

C. selecting strains, wherein obvious transparent circles can appear around colonies on the surface of a casein plate after culture, dyeing a CMC-Na plate for 30min by using 0.2% congo red, then completely washing off a dye solution by using distilled water and 1mol/L NaCl in sequence, wherein the transparent circles can be seen around the colonies, respectively selecting 5 colonies with the diameters of the transparent circles larger than that of the colonies on the two plates, carrying out continuous streak culture until the colonies are pure, and inoculating the purified strains into a nutrient agar culture medium for storage and later use.

Further, the step (2) specifically comprises the steps of putting 2-3 parts of bagasse, 2-3 parts of agilawood leaves and 7-10 parts of water into a container, mixing, adding cellulase with an OD600 (OD 600) of 0.6-0.8, which is 0.1-0.2% of the volume of slurry, a protease high-yield strain culture solution and a bacillus megaterium culture solution into the container, and sealing. Fermenting at room temperature for 2 months.

Further, the step (5) is that 3-5 parts of ferment filtrate, 18-22 parts of plant surfactant and 1-4 parts of acid additive are mixed according to the weight proportion; 0.1-0.5 part of caustic soda flakes; 0.3-0.5 part of chelating agent; 0.1-0.5 part of preservative; 1-1.5 parts of a thickening agent; 0.5-1 part of anti-redeposition agent; and mixing 67-74 parts of deionized water to prepare the detergent.

The invention has the beneficial effects that: the process has the advantages that waste materials are fully utilized, the energy utilization rate is improved, the resources of the aquilaria sinensis leaves and bagasse are fully utilized, waste materials are changed into valuable materials, a good detergent is produced, the purposes of resource utilization and environmental pollution avoidance are achieved, the biological fermentation washing liquid is rich in plant care essence, the formula is mild, no harm is caused to fabric fibers and skins, the cleaning power of the biological fermentation washing liquid is strong, specific dirt separation factors and bioactive enzymes are rich, stubborn stains inside the clothes fibers and on the household surface are thoroughly decomposed, and the foaming capacity of the biological fermentation washing liquid is strong. Easy rinsing and more worry-saving washing, wherein the rich potassium ions have the function of promoting foaming, and the biological fermentation washing liquid has stronger sterilization function. The biological fermentation cleaning solution can effectively kill escherichia coli, staphylococcus and some harmful bacteria, can effectively inhibit reproduction and generation of bacterial spores, has the advantages of cheap raw materials, environment-friendly process, less emission and the like, and is very suitable for commercial application.

Detailed Description

The invention is illustrated below by means of specific examples, without being restricted thereto.