阅读说明：本技术 毒品的远红外检测方法 (Far infrared detection method for drugs ) 是由 马霞 张志远 田桢干 于 2019-11-18 设计创作，主要内容包括：本发明公开了一种毒品的远红外检测方法,所述毒品包括可卡因、盐酸氯胺酮、大麻酚、吗啡、海洛因。毒品的远红外检测方法包括如下步骤：1.制备毒品标准品样品；2.应用远红外检测技术对毒品标准品进行检测并构建标准指纹谱库；3.利用构建的标准指纹谱库对未知物进行检测。本发明通过应用远红外检测技术构建五种毒品指纹图谱库并利用指纹谱库能快速检测未知物是否为上述毒品。远红外波光子能量低穿透力强,不会对人体造成伤害可实现无损检测。(The invention discloses a far infrared detection method of drugs, wherein the drugs comprise ***e, ketamine hydrochloride, cannabinol, morphine and heroin. The far infrared detection method of the drugs comprises the following steps: 1. preparing a drug standard sample; 2. detecting the drug standard substance by using a far infrared detection technology and constructing a standard fingerprint spectrum library; 3. and detecting the unknown substance by using the constructed standard fingerprint spectrum library. The invention constructs five drug fingerprint spectrum libraries by applying a far infrared detection technology and can quickly detect whether an unknown substance is the drug by utilizing the fingerprint spectrum libraries. The far infrared wave photon energy is low, the penetrating power is strong, and the nondestructive detection can be realized without causing damage to human bodies.)

1. A far infrared detection method for drugs is characterized by comprising the following steps:

step 1: preparing a drug standard substance;

step 2: detecting a drug standard substance by using a far infrared detection technology and constructing a standard substance fingerprint atlas database;

and step 3: and detecting the unknown substance by using the constructed standard fingerprint atlas library.

2. The far infrared detection method for drugs according to claim 1, wherein the drug in step 1 is one of ***e, ketamine hydrochloride, cannabinol, morphine or heroin.

3. The far infrared detection method for drugs according to claim 1, wherein the step 1 specifically comprises: preparing the drug into a liquid standard substance with the concentration of 1mg/mL and the volume of 1mL, dripping the liquid standard substance on a filter membrane with the diameter of 13mm, and drying at 40 ℃ to obtain the drug standard substance.

4. The far infrared detection method of drugs according to claim 1, wherein in the step 2, the testing environment of the far infrared detection technology is vacuum condition, the temperature is 21 ℃, and the frequency range of the far infrared absorption spectrum of the drug standard is 30-350cm^-1(ii) a The instrument scans 128 times with a resolution of 2cm^-1(ii) a Each sample is tested 8 times under the same condition, and the average value is taken as the signal of the sample, so that human errors are eliminated, and the signal-to-noise ratio is improved.

5. The far infrared detection method for drugs of claim 1, wherein the characteristic absorption of the fingerprint of ***e in the standard fingerprint library of step 2 is 50.94, 71.59, 119.54, 167.16, 191.53, 240.42, 284.71, 300.03cm^-1To (3).

6. The far infrared detection method for drugs of abuse of claim 1, wherein the characteristic absorption of the fingerprint of ketamine hydrochloride in the standard fingerprint spectrum library in step 2 is 97.90, 145.18, 200.79, 232.76, 260.73, 286.71, 341.65cm^-1To (3).

7. The far infrared method for detecting drugs according to claim 1, wherein the characteristic absorption of the fingerprint of cannabinol in the standard fingerprint library of step 2 is 45.95, 72.92, 101.02, 143.19, 189.06, 224.25, 234.76, 256.98, 286.81, 298.36, 321.46cm^-1To (3).

8. The far infrared method for detecting drugs according to claim 1, wherein the characteristic absorption of the fingerprint of morphine in the standard fingerprint library of step 2 is 50.48, 65.38, 80.77, 99.04, 124.04, 67.80, 181.74, 197.61, 237.51, 274.54, 288.48, 302.90, 319.01cm^-1To (3).

9. The far infrared detection method for drugs according to claim 1, wherein the characteristic absorption of the heroin fingerprint in the standard fingerprint library of step 2 is 36.57, 46.19, 66.41, 95.43, 139.93, 185.75, 199.23, 245.41, 267.52, 283.95, 315.69cm^-1To (3).

Technical Field

The invention belongs to the technical field of drug detection, and particularly relates to a detection method for constructing a drug standard fingerprint spectrum by using far infrared waves.

Background

Drugs seriously harm human health and social peace, and are a public nuisance. The drug addicts have a very strong craving for drugs after addiction, and in order to meet the requirement that the drug addicts can obtain the drugs without means, thereby seriously disturbing social security and causing national and national crisis. Prevention and control of drug abuse is an important content of government work, and how to quickly discover and detect drugs is a difficult problem which needs to be solved urgently by drug enforcement and drug prohibition in customs, public security and other departments.

Some existing technologies for detecting drugs include: chemical methods, spectroscopic methods, ion mobility spectrometry, chromatography, mass spectrometry, and combination techniques, among others. Each of these methods has advantages, but all have limitations. For example, drugs with low content and similar chemical structure are not easy to detect by a chemical detection method, and the contained impurities affect the accuracy of the detection result, so that the method is mostly used in laboratories and cannot realize rapid detection. Drugs can only see the external shape under X-ray, cannot be qualitatively analyzed, and the X-ray has certain radiation hazard to human bodies, and especially sensitive people such as pregnant women, children and the like need to be prevented from being exposed to the X-ray as far as possible. Therefore, X-rays are often used only for article detection and not for human body detection. Ion mobility spectrometry has low resolution and poor quantitativity, and can only detect in a relatively low concentration range. The chromatography and mass spectrometry are complex in operation and high in cost, and are not suitable for on-site detection of drugs.

The far infrared radiation has low photon energy, shows higher specificity to different configurations or crystal form changes of chemical molecules, vibration or rotation modes, intermolecular force and the like, and is suitable for qualitative identification of molecules with different types or similar structures. In addition, the far infrared light wave can penetrate through various packaging materials, and nondestructive testing can be performed on drugs.

Disclosure of Invention

The technical problem to be solved by the invention is as follows: the far infrared detection method for the drugs is provided, and is used for detecting drug standard samples, constructing a standard fingerprint spectrum library and judging whether the position samples are the drugs and the types of the drugs.

In order to solve the technical problem, the invention provides a far infrared detection method of drugs, which is characterized by comprising the following steps:

step 1: preparing a drug standard substance;

step 2: detecting a drug standard substance by using a far infrared detection technology and constructing a standard substance fingerprint atlas database;

and step 3: and detecting the unknown substance by using the constructed standard fingerprint atlas library.

Preferably, the drug in step 1 is one of ***e, ketamine hydrochloride, cannabinol, morphine or heroin.

Preferably, the step 1 specifically includes: preparing the drug into a liquid standard substance with the concentration of 1mg/mL and the volume of 1mL, dripping the liquid standard substance on a filter membrane with the diameter of 13mm, and drying at 40 ℃ to obtain the drug standard substance.

Preferably, in the step 2, the testing environment of the far infrared detection technology is under vacuum condition, the temperature is 21 ℃, and the frequency range of the far infrared absorption spectrum of the drug standard is 30-350cm^-1(ii) a The instrument scans 128 times with a resolution of 2cm^-1(ii) a Each sample is tested 8 times under the same condition, and the average value is taken as the signal of the sample, so that human errors are eliminated, and the signal-to-noise ratio is improved.

Preferably, the characteristic absorption of the ***e fingerprint in the standard fingerprint spectrum library in the step 2 is 50.94, 71.59, 119.54, 167.16, 191.53, 240.42, 284.71 and 300.03cm^-1To (3).

Preferably, the characteristic absorption of the fingerprint pattern of ketamine hydrochloride in the standard fingerprint pattern library in the step 2 is 97.90, 145.18, 200.79, 232.76, 260.73, 286.71 and 341.65cm^-1To (3).

Preferably, the characteristic absorption of the fingerprint of cannabinol in the standard fingerprint spectrum library in step 2 is 45.95, 72.92, 101.02, 143.19, 189.06, 224.25, 234.76, 256.98, 286.81, 298.36 and 321.46cm^-1To (3).

Preferably, the characteristic absorption of the morphine fingerprint in the standard fingerprint spectrum library in the step 2 is 50.48, 65.38, 80.77, 99.04, 124.04, 67.80, 181.74, 197.61, 237.51, 274.54, 288.48, 302.90 and 319.01cm^-1To (3).

Preferably, the characteristic absorption of the heroin fingerprint in the standard fingerprint spectrum library in the step 2 is 36.57, 46.19, 66.41, 95.43, 139.93, 185.75, 199.23, 245.41, 267.52, 283.95 and 315.69cm^-1To (3).

Compared with the prior art, the invention has the beneficial effects that:

the technical method of the invention is used to obtain the far infrared characteristic absorption spectra of five drugs, and a standard fingerprint spectrum library is established, namely the standard for detecting unknown samples. Compared with the traditional drug detection means, the far infrared technology has the greatest advantage that the far infrared technology has strong penetrating power, can penetrate through non-polar materials such as cartons, plastics, cloth, wood and the like for packaging, and therefore can realize the rapid nondestructive detection of drugs under the condition of not opening the package, thereby shortening the detection time and improving the detection efficiency. The far infrared detection technology overcomes the defects of the existing detection technology means, and can accurately judge the types of drugs in the packaging materials. In addition, the photon energy of the far infrared wave is low, so that the ionization phenomenon can not be generated, the detected substance can not be damaged, and the personal injury to the detection personnel can not be caused.

Drawings

FIG. 1: an optical path diagram of a far infrared band of a Fourier transform spectrometer;

FIG. 2: a far infrared fingerprint spectrum of ***e;

FIG. 3: the far infrared fingerprint spectrum of ketamine hydrochloride;

FIG. 4: a far infrared fingerprint of cannabinol;

FIG. 5: far infrared fingerprint spectrum of morphine;

FIG. 6: far infrared fingerprint spectrum of heroin.

Detailed Description

The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teaching of the present invention, and such equivalents may fall within the scope of the present invention as defined in the appended claims.

The five drugs were tested on a Fourier transform spectrometer (FTIR) as shown in FIG. 1, a Fourier transform infrared spectrometer model Vertex80V from Bruker. The system resolution is larger than 13.5GHz, and the signal-to-noise ratio is better than 50000: 1, the instrument is provided with a sample cavity, and the instrument is vacuumized during measurement so as to reduce the influence of moisture in the air on a test result and improve the signal-to-noise ratio. When the test is carried out in the far infrared wave band,the far infrared beam splitter needs to be replaced. There are different beam splitters for different wavebands: 6 μm Mylar film (500-100 cm)^-1) 25 μm Myalr membrane (135-40 cm)^-1) 50 μm Mylar film (90-25 cm)^-1) 125 mu m Mylar film (30-4 cm)^-1) Because of the different precision in each frequency band, the frequency band range of the sample can be selectively reduced or enlarged.

The FTIR system adopted by the invention mainly comprises a light source (mercury lamp), a beam splitter, an interferometer, a detector, a computer and the like, and the working principle of the FTIR system is as shown in figure 1. The mercury lamp light source emits stable and high-intensity infrared light with continuous wavelength, the infrared light is collected and reflected through the parabolic mirrors P1, P2 and P3, reflected light reaches the collimating mirror M2 through the parabolic mirror P4 after passing through the diaphragm, parallel reflected light reflected from the collimating mirror is emitted to the interferometer, the parallel interference light emitted from the interferometer is emitted to the sample chamber after being reflected through the parabolic mirror P5, when the vibration frequency of a certain group in a sample molecule is the same as the far infrared light frequency, resonance can be generated, and at the moment, the group can absorb the far infrared light with certain frequency. Far infrared light penetrating through the sample reaches the detector after being focused by the parabolic mirror P6, the condition that molecules absorb the far infrared light is recorded by the detector, namely the change of light intensity, an interference pattern is obtained, Fourier mathematical transformation processing is carried out by a computer, and finally a far infrared spectrogram of the sample is obtained.

Measuring five standard samples of the drugs by utilizing FTIR far infrared wave band to obtain the five standard samples of the drugs in the far infrared wave band (30-350 cm)^-1) A fingerprint spectrum. Most of drugs with real names in a large number of theories and experiments have obvious fingerprint characteristics continuously at far infrared, and the absorption peak of the far infrared corresponds to the vibration or rotation energy level of molecules and is 30-350cm^-1In the frequency range, each drug has a plurality of characteristic absorption peaks related to the molecular structure or the crystal state, the absorption peak positions of different drugs are different, and the absorption peak positions of the same drug are kept unchanged at different time, environments and states. Therefore, five standard samples of drugs including ***e, ketamine hydrochloride, cannabinol, morphine and heroin were tested using the above method.

The preparation method is adopted when preparing five drug standard substancesThe samples were identical, i.e. they were prepared by dropping the liquid standard on a filter membrane with a diameter of 13mm and drying at 40 ℃. And (3) putting the dried sample into a sample groove of an instrument, and detecting at the temperature of 21 ℃ under a vacuum condition. The far infrared absorption spectrum of the drug standard substance has a frequency range of 30-350cm^-1. The instrument scans 128 times with a resolution of 2cm^-1. Each sample is tested 8 times under the same condition, and the average value is taken as the signal of the sample, so that human errors are eliminated, and the signal-to-noise ratio is improved. And (3) carrying out data acquisition by using the self-contained software OPUS6.5 of the instrument and carrying out relevant preprocessing.