阅读说明：本技术 光激化学发光检测装置 (Light-activated chemiluminescence detection device ) 是由 方泉 练子富 赵卫国 刘宇卉 李临 于 2018-08-10 设计创作，主要内容包括：本发明涉及一种光激化学发光检测装置,涉及化学发光免疫分析技术领域,用于解决现有技术中存在无法避免因HOOK效应造成误报检测结果的技术问题。本发明的光激化学发光检测装置,包括孵育装置和检测机构,孵育装置通过旋转将同一待测物质多次移动到检测位置,使得检测机构对待测物质进行多次检测,进而判断免疫测定是否存在HOOK风险,避免HOOK效应所导致的被检测样本不能被正确区分是由于其浓度超出检测试剂盒的线性范围还是本身浓度就是该值,从而避免实验误诊。(The invention relates to a light-activated chemiluminescence detection device, relates to the technical field of chemiluminescence immunoassay, and is used for solving the technical problem that false alarm detection results caused by the HOOK effect cannot be avoided in the prior art. The light-activated chemiluminescence detection device comprises an incubation device and a detection mechanism, wherein the incubation device moves the same substance to be detected to a detection position for multiple times through rotation, so that the detection mechanism detects the substance to be detected for multiple times, and further judges whether the immunoassay has a HOOK risk, thereby avoiding the condition that the concentration of the detected sample cannot be correctly distinguished because the concentration exceeds the linear range of the detection kit or the concentration is the value caused by the HOOK effect, and further avoiding misdiagnosis in experiments.)

1. A light activated chemiluminescent detection device comprising:

the incubation device is used for incubating the substance to be tested; the incubation device moves the same substance to be detected to the detection position for multiple times through periodic motion;

and the detection mechanism is arranged on one side of the incubation device and is used for carrying out multiple light excitations on the substance to be detected at the detection position and detecting chemiluminescence generated by the substance to be detected after each light excitation.

2. The apparatus of claim 1, wherein the detection mechanism comprises an excitation portion for emitting excitation light and exciting the analyte, and a detection portion for receiving and detecting a luminescence signal generated by the analyte.

3. The apparatus of claim 2, wherein the excitation portion and the detection portion do not operate simultaneously.

4. The light-activated chemiluminescence detection device according to claim 2 or 3, wherein the excitation portion comprises an exciter capable of emitting 600-700 nm red excitation light.

5. The apparatus according to claim 4, wherein the detection unit includes a detector, and the detector is a single-photon counter, a photomultiplier tube, a silicon photocell, or a photometric integrating sphere.

6. The apparatus according to claim 2 or 3, wherein the wavelength of the luminescence signal detectable by the detecting section is 520 to 620 nm.

7. The light-activated chemiluminescent detection device of any one of claims 1-3 wherein the detection mechanism is disposed above the incubation device.

8. The light-activated chemiluminescent detection device of claim 5 wherein the excitation portion comprises an excitation light pathway and the detection portion comprises a signal light pathway, the excitation light pathway and the signal light pathway being neither simultaneously on nor off.

9. The apparatus according to claim 8, wherein a first switch is disposed on the excitation light path for controlling the activation or deactivation of the excitation light path, a second switch is disposed on the signal light path for controlling the activation or deactivation of the signal light path, and the first switch and the second switch are linked in reverse.

10. The apparatus of claim 9, wherein the first switch and the second switch are connected to two ends of a driving unit, and the driving unit drives the first switch and the second switch to be linked in opposite directions.

11. The apparatus of claim 10, wherein the first switch comprises a shaft, and a first through hole is disposed on the shaft in a radial direction of the shaft, and the first through hole is periodically aligned with the excitation light path.

12. The apparatus of claim 9, wherein the second switch comprises a crank mechanism, the crank mechanism having a second through hole disposed therein, the second through hole periodically aligned with the signal light path.

13. The light-activated chemiluminescent detection device of claim 12 wherein the crank mechanism comprises a first rotating plate and a second rotating plate hinged to each other, the first rotating plate being connected to the driving portion and the second through hole being provided at a lower portion of the second rotating plate.

14. The photoluminescence detection device of claim 9, wherein the excitation portion further comprises a second lens and a transflective lens, the exciter is disposed above the excitation light path, the second lens is disposed between the exciter and the first switch, and the transflective lens is disposed below the first switch.

15. The apparatus according to claim 9, wherein the detection portion further comprises a first lens and a filter, the detector is disposed on one side of the signal light path, and the first lens and the filter are sequentially disposed between the transflective lens and the detector.

16. The light-activated chemiluminescent detection device of claim 8 wherein the excitation light passage and the signal light passage are both disposed on a housing with the axis of the excitation light passage perpendicular to the axis of the signal light passage.

17. The light-activated chemiluminescent detection device of claim 16 wherein the housing comprises a lower base disposed on the incubation device, an upper base fixed on the lower base and a baffle disposed on the side of the lower base, the excitation light path runs through the upper base and the lower base, the signal light path runs through the side wall of the lower base and the baffle.

18. The apparatus of claim 17, wherein the upper base is provided with an exciter holder for fixing the exciter and a lens holder for fixing the second lens.

19. The apparatus according to any one of claims 1 to 3, wherein the incubating apparatus comprises a reagent chamber for accommodating the reaction cup and a rotating member for rotating the reagent chamber, and the detecting mechanism is fixed at a detecting position on the reagent chamber.

20. The apparatus of claim 19, wherein a fixing device is disposed inside the reagent chamber, the reaction cup is disposed on the fixing device, and the fixing device rotates with the reagent chamber.

21. The apparatus of claim 20, wherein the fixing means is configured in a disc shape, fixing grooves are formed at equal intervals on the circumference of the fixing means, and the reaction cups are disposed in the fixing grooves.

22. The apparatus of claim 20, wherein the rotation member comprises a support, and a rotation shaft and a motor respectively disposed on the support, the motor is connected to the rotation shaft through a synchronous belt, and the rotation shaft is connected to the rotation connection portion at the bottom of the reagent chamber.

23. The apparatus according to claim 22, wherein the supporting body has a mounting hole, and a supporting column is disposed in the mounting hole and connected to the bottom of the reagent chamber.

24. The light-activated chemiluminescent detection device of claim 23 wherein the rotating member is provided with a positioning device for reading the position of the reagent cartridge.

25. The apparatus of claim 24, wherein the positioning device comprises a sensor fixed on the support and a sensor flag disposed at the bottom of the rotating shaft, the sensor flag being flush with the height of the sensor and rotating with the rotating shaft.

Technical Field

The invention relates to the technical field of chemiluminescence immunoassay, in particular to a light-activated chemiluminescence detection device.

Background

Chemiluminescence immunoassay is a non-radioactive immunoassay which is developed rapidly in recent years, and the principle is that a chemiluminescence substance is used for amplifying signals and an immunological binding process is directly measured by virtue of the luminous intensity, and the method is one of important directions of immunological detection. In the double-antibody sandwich detection mode, when the concentration of a substance to be detected is higher than a certain concentration, a phenomenon that a double-antibody sandwich compound cannot be formed so that a signal value is lower is called a high dose-HOOK effect (HD-HOOK effect), and the phenomenon that false alarm detection results are caused by the HOOK effect is difficult to avoid in the existing chemiluminescence instruments.

Disclosure of Invention

The invention provides a light-activated chemiluminescence detection device, which is used for solving the technical problem that false alarm detection results caused by the HOOK effect cannot be avoided in the prior art.

The invention provides a light-activated chemiluminescence detection device, which comprises:

the incubation device is used for incubating the substance to be tested; the incubation device moves the same substance to be detected to the detection position for multiple times through periodic motion;

and the detection mechanism is arranged on one side of the incubation device and is used for carrying out multiple light excitations on the substance to be detected at the detection position and detecting chemiluminescence generated by the substance to be detected after each light excitation.

In one embodiment, the detection mechanism includes an excitation portion for emitting excitation light and exciting the analyte, and a detection portion for receiving and detecting a luminescence signal generated by the analyte.

In one embodiment, the excitation portion and the detection portion do not operate simultaneously.

In one embodiment, the excitation part comprises an exciter capable of emitting red excitation light of 600-700 nm.

In one embodiment, the detection section includes a detector which is a single photon counter, a photomultiplier tube, a silicon photocell, or a photometric integrating sphere.

In one embodiment, the wavelength of the emission signal detectable by the detection unit is 520 to 620 nm.

In one embodiment, the detection mechanism is disposed above the incubation device.

In one embodiment, the excitation portion includes an excitation light path, and the detection portion includes a signal light path, the excitation light path being on and off at different times from the signal light path.

In one embodiment, a first switch for controlling the excitation light path to be turned on or off is disposed on the excitation light path, a second switch for controlling the signal light path to be turned on or off is disposed on the signal light path, and the first switch and the second switch are linked in reverse.

In one embodiment, the first switch and the second switch are connected to two ends of a driving part, and the driving part enables the first switch and the second switch to be linked in opposite directions.

In one embodiment, the first switch includes a rotating shaft, and the rotating shaft is provided with a first through hole penetrating through the rotating shaft in a radial direction, and the first through hole is periodically aligned with the excitation light passage.

In one embodiment, the second switch includes a crank mechanism having a second through hole disposed therein, the second through hole periodically aligned with the signal light path.

In one embodiment, the crank mechanism includes a first rotation plate and a second rotation plate hinged to each other, the first rotation plate is connected to the driving part, and the second through hole is provided at a lower portion of the second rotation plate.

In one embodiment, the excitation portion further includes a second lens and a half mirror, the exciter is disposed above the excitation light path, the second lens is disposed between the exciter and the first switch, and the half mirror is disposed below the first switch.

In one embodiment, the detection unit further includes a first lens and a filter, the detector is disposed on one side of the signal light path, and the first lens and the filter are sequentially disposed between the half mirror and the detector.

In one embodiment, the excitation light passage and the signal light passage are both provided on a housing, and an axis of the excitation light passage is perpendicular to an axis of the signal light passage.

In one embodiment, the housing includes a lower base disposed on the incubation device, an upper base fixed on the lower base, and a baffle disposed at a side of the lower base, the excitation light path penetrates through the upper base and the lower base, and the signal light path penetrates through a sidewall of the lower base and the baffle.

In one embodiment, the upper base is provided with an exciter holder for fixing the exciter and a lens holder for fixing the second lens.

In one embodiment, the incubation device comprises a reagent bin for accommodating the reaction cup and a rotating component for driving the reagent bin to rotate, and the detection mechanism is fixed at a detection position on the reagent bin.

In one embodiment, a fixing device is arranged inside the reagent bin, the reaction cup is arranged on the fixing device, and the fixing device rotates along with the reagent bin.

In one embodiment, the fixing device is configured in a disk shape, fixing grooves are provided at equal intervals on the circumference of the fixing device, and the reaction cups are disposed in the fixing grooves.

In one embodiment, the rotating component comprises a support body, and a rotating shaft and a motor which are respectively arranged on the support body, wherein the motor is connected with the rotating shaft through a synchronous belt, and the rotating shaft is connected with a rotating connecting part at the bottom of the reagent cabin.

In one embodiment, the support body is provided with a mounting hole, and a support column connected with the bottom of the reagent cabin is arranged in the mounting hole.

In one embodiment, the rotating member is provided with a positioning device for reading the position of the reagent cartridge.

In one embodiment, the positioning device comprises a sensor fixed on the support body and a sensor flag arranged at the bottom of the rotating shaft, and the sensor flag is flush with the height of the sensor and rotates along with the rotating shaft.

Compared with the prior art, the invention has the advantages that: the incubation device moves the same substance to be detected to the detection position for multiple times through rotation, so that the detection mechanism detects the substance to be detected for multiple times, and then judges whether the immunoassay has a HOOK risk, and avoids the condition that the detected sample caused by the HOOK effect cannot be correctly distinguished because the concentration of the detected sample exceeds the linear range of the detection kit or the concentration of the detected sample is the value, thereby avoiding misdiagnosis in experiments.

Drawings

The invention will be described in more detail hereinafter on the basis of embodiments and with reference to the accompanying drawings.

FIG. 1 is a perspective view of an optical chemiluminescence detection apparatus according to an embodiment of the invention;

FIG. 2 is a top view of an embodiment of the light-activated chemiluminescent detection device of the present invention;

FIG. 3 is a perspective view of the detection mechanism shown in FIG. 1;

FIG. 4 is a front view of the detection mechanism shown in FIG. 1;

FIG. 5 is a cross-sectional view (cross-sectional line is not shown) taken along line A-A when the excitation light path is turned on in the detection mechanism shown in FIG. 4;

FIG. 6 is a cross-sectional view (cross-sectional line is not shown) at A-A when the passage of excitation light is closed in the detection mechanism shown in FIG. 4;

FIG. 7 is a cross-sectional view (cross-sectional line not shown) taken at B-B when the signal light path is turned on in the detection mechanism shown in FIG. 4;

fig. 8 is a cross-sectional view (cross-sectional line not shown) at B-B when the signal light path is closed in the detection mechanism shown in fig. 4.

In the drawings, like components are denoted by like reference numerals. The figures are not drawn to scale.

Reference numerals:

1-an incubation device; 2-a detection mechanism; 3-an excitation section;

4-a detection section; 5-a shell; 6-reagent cabin;

7-a rotating member; 8-a positioning device; 31-excitation light path;

32-a first switch; 33-an exciter; 34-a second lens;

35-a semi-transparent semi-reflective lens; 41-signal light path; 42-a second switch;

43-a detector; 44-a first lens; 45-optical filters;

51-a drive section; 52-a lower base; 53-upper base;

54-a baffle; 61-a fixation device; 62-reaction cup;

63-fixing groove; 71-a support; 72-a rotating shaft;

73-a motor; 74-a synchronous belt; 75-support column;

81-a sensor; 82-sensor flag; 321-a rotating shaft;

322-a first via; 421-crank mechanism; 422-a second via;

422-a first rotating plate; 423-second rotating plate; 531-exciter mount;

532-lens holder; 711-mounting holes.

Detailed Description

The invention will be further explained with reference to the drawings.

As shown in fig. 1 and 2, the present invention provides a light-activated chemiluminescence detection apparatus, which includes an incubation apparatus 1 and a detection mechanism 2, wherein the incubation apparatus 1 is configured to incubate a substance to be detected and move the same substance to be detected to a detection position multiple times through periodic motion; the detection mechanism 2 is arranged on one side of the incubation device 1 and is used for carrying out multiple light excitations on the substance to be detected at the detection position and detecting chemiluminescence generated by the substance to be detected after each light excitation.

Specifically, the incubation device 1 rotates to move the same substance to be detected to the detection position for multiple times, so that the detection mechanism 2 detects the substance to be detected for multiple times, and then judges whether the immunoassay has a HOOK risk.

Preferably, the detection mechanism 2 is disposed above the incubation device 1, so as to facilitate the emission of excitation light to the substance to be measured.

Optionally, the detection means 2 is arranged at the side or bottom of the incubation device 1.

In some embodiments, the detection position refers to a position where the detection mechanism is located (i.e., a position where the excitation light is generated).

Of course, the detection position may be a position on the incubation device 1 where the substance to be detected is located.

It should be noted that the periodic motion of the present invention includes rotation, linear reciprocating motion or oscillation.

The detection mechanism 2 of the present invention will be described in detail below.

As shown in fig. 3 to 8, the detection mechanism 2 includes an excitation portion 3 for emitting excitation light and exciting the object to be measured and a detection portion 4 for receiving and detecting a luminescence signal generated by the object to be measured, and the excitation portion 3 and the detection portion 4 do not operate at the same time.

In one embodiment, the excitation section 3 comprises an exciter 33, said exciter 33 being capable of emitting red excitation light of 600-700 nm.

The exciter 33 is disposed above the substance to be measured, and in the excitation portion 3, except that the exciter 33 does not move periodically with the incubation device 1, the rest of the parts of the excitation portion 3 can move periodically with the incubation device 1, which is not limited in the present invention.

In one embodiment, the detection section 4 includes a detector 43, wherein the detector 43 is a single photon counter, a photomultiplier tube, a silicon photocell, or a photometric integrating sphere.

Wherein the wavelength of the light emission signal that can be detected by the detection section 4 is 520 to 620 nm.

Similarly, in the detecting section 4, the other components of the detecting section 4 may move periodically with the incubator 1, except that the detector 43 does not move periodically with the incubator 1, and the present invention is not limited thereto.

Further, as shown in fig. 3, the excitation portion 3 includes an excitation light path 31, and the detection portion 4 includes a signal light path 41, and the excitation light path 31 and the signal light path 41 are not simultaneously turned on and off.

The excitation light path 31 is provided with a first switch 32 for controlling the on/off of the excitation light path 31, the signal light path 41 is provided with a second switch 42 for controlling the on/off of the signal light path 41, and the first switch 32 and the second switch 42 are linked in reverse direction. Thereby simultaneously driving the excitation light path 31 and the signal light path 41 to open and close, as follows: when the excitation light path 31 is opened, the signal light path 41 is closed; when the excitation light path 31 is closed, the signal light path 41 is opened.

Specifically, when the excitation light is required to excite the object to be measured, the driving portion 51 rotates, the driving portion 51 drives the first switch 32 to rotate, the excitation light path 31 is turned on (as shown in fig. 5), and simultaneously the driving portion 51 drives the second switch 42 to rotate, and the signal light path 41 is in the off state (as shown in fig. 8).

Similarly, when the light emitting signal generated by the object to be detected is received and detected, the driving portion 51 rotates again, the driving portion 51 drives the first switch 32 to rotate, the first switch 32 blocks the excitation light path 31 (as shown in fig. 6), and simultaneously the driving portion 51 drives the second switch 42 to rotate, and the signal light path 41 is in an open state (as shown in fig. 7). Thus, the driving section 51 controls the opening and closing of the excitation light path 31 and the signal light path 41 at the same time.

As shown in fig. 3, the first switch 32 and the second switch 42 are connected to both ends of the driving unit 51, respectively, and the driving unit 51 causes the first switch 32 and the second switch 42 to be interlocked in opposite directions.

The driving unit 51 is a rotary electromagnet or a motor. The driving portion 51 has output shafts respectively provided at both ends thereof, one end of which is connected to the first switch 32 and the other end of which is connected to the second switch 42.

The first switch 32 includes a rotating shaft 321, a first through hole 322 penetrating the rotating shaft 321 in a radial direction is disposed on the rotating shaft 321, and the first through hole 322 is periodically aligned with the excitation light path 31.

Specifically, as shown in fig. 5, the driving portion 51 drives the rotating shaft 321 to rotate, when the first through hole 322, to which the rotating shaft 321 rotates, is aligned with the excitation light path 31, the excitation light path 31 is conducted, and the excitation light emitted by the excitation portion 3 can irradiate on the substance to be measured; as shown in fig. 6, when the first through hole 322 to which the rotation shaft 321 is rotated is misaligned with the excitation light path 31, the excitation light path 31 is closed, and the excitation light emitted from the excitation portion 3 cannot be irradiated on the material to be measured.

The second switch 42 includes a crank mechanism 421, and a second through hole 422 is disposed on the crank mechanism 421, and the second through hole 422 is periodically aligned with the signal light path 41.

The crank mechanism 421 includes a first rotating plate 423 and a second rotating plate 423 hinged to each other, the first rotating plate 423 being connected to the driving part 51, and a second through hole 422 being provided at a lower portion of the second rotating plate 423.

Specifically, as shown in fig. 8, when the driving portion 51 drives the second rotating plate 423 to rotate around the rotation center thereof counterclockwise, the second rotating plate 423 drives the first rotating plate 424 to rotate around the rotation center thereof clockwise, so that the second through hole 422 aligns with the signal light path 41, the signal light path 41 is conducted, and the light-emitting signal generated by the object to be detected enters the detecting portion 4 for detection; as shown in fig. 7, the driving portion 51 drives the second rotating plate 423 to rotate clockwise, so that the first rotating plate 424 rotates counterclockwise around the rotation center thereof, the second through hole 422 is misaligned with the signal light path 41, and the signal light path 41 is closed.

When the driving portion 51 drives the rotating shaft 321 to rotate clockwise, the first through hole 322 is aligned with the excitation light path 31, and simultaneously the driving portion 51 rotates the first rotating plate 424 counterclockwise, and the second through hole 422 is misaligned with the signal light path 41; similarly, when the driving portion 51 drives the rotating shaft 321 to rotate counterclockwise, and the first through hole 322 is dislocated from the excitation light path 31, the driving portion 51 makes the first rotating plate 424 rotate clockwise, and the second through hole 422 is aligned to the signal light path 41, so as to ensure that the process of detecting the light-emitting signal generated by the object to be detected and the process of exciting the object to be detected by the excitation light do not interfere with each other, thereby improving the accuracy of the detection information.

As shown in fig. 5, the excitation section 3 further includes a second lens 34 and a half mirror 35, the exciter 33 is disposed above the excitation light path 31, the second lens 34 is disposed between the exciter 33 and the first switch 32, and the half mirror 35 is disposed below the first switch 32.

The exciting light emitted by the exciter 33 excites the object to be detected for multiple times, so that the object to be detected generates multiple light-emitting signals; the second lens 34 is used for focusing the excitation light; the half mirror 35 can not only cut off the excitation light with the non-target wavelength by the excitation light with the target wavelength, but also reflect the emission signal with the target wavelength generated by the object to be measured.

The detection section 4 further includes a first lens 44 and a filter 45, the detector 43 is disposed on one side of the signal light path 41, and the first lens 44 and the filter 45 are sequentially disposed between the half mirror 35 and the detector 43.

Wherein the detector 43 detects the luminescence signal generated by the object to be detected for a plurality of times and records the corresponding detection result.

The light emission signal generated by the object to be measured reflected by the half mirror 35 can enter the detection section 4 through the first lens 44. The light emitting signal generated by the object to be detected is reflected by the half-mirror 35 and then enters the detecting part 4 through the first lens 44 and the filter 45 in sequence. The filter 45 can extract a signal with a desired wavelength from the light-emitting signal generated by the object to be measured, and cut off the stray light signals except the wavelength.

Wherein, the excitation light passage 31 and the signal light passage 41 are both disposed on the housing 5, and the axis of the excitation light passage 31 is perpendicular to the axis of the signal light passage 41. As shown in fig. 3, the axis L1 of the excitation light path 31 is in the Z-axis direction, and the axis L2 of the signal light path 41 is in the X-axis direction.

The housing 5 includes a lower base 52 provided on the incubation device 1, an upper base 53 fixed to the lower base 52, and a baffle 54 provided on the side of the lower base 52, the excitation light path 31 penetrates the upper base 53 and the lower base 52, and the signal light path 41 penetrates the side wall of the lower base 52 and the baffle 54.

Wherein the excitation light passage 31 includes a first excitation light passage (e.g., a through hole) penetrating the upper base 53 and a second excitation light passage (e.g., a through hole) penetrating the lower base 52, and axes of the first and second excitation light passages coincide with each other; the signal light path 41 includes a first signal light path (e.g., a through hole) penetrating the sidewall of the lower base 52 and a second signal light path (e.g., a through hole) penetrating the shutter 54, and axes of the first signal light path and the second signal light path coincide with each other.

As shown in fig. 4, the barrier 54 is disposed at one side of the lower base 52, the second switch 42 is disposed between the barrier and the lower base 52, and the detector 43 and the second switch 42 are disposed at the outer side and the inner side of the barrier 54, respectively.

The end of the rotating shaft 321 with the first through hole 322 penetrates through the upper base 53, the first through hole 322 is disposed inside the upper base 53, and the end of the rotating shaft 321 is rotatably connected with a side wall of one side of the upper base 53. When the excitation light needs to be controlled to excite the object to be detected, the driving portion 51 rotates, and the output shaft at the first end thereof drives the rotating shaft 321 to rotate, so that the first through hole 322 is aligned with the excitation light passage 31, and the excitation light emitted by the exciter 33 passes through the excitation light passage 31 to excite the object to be detected (at this time, the signal light passage 41 is in a closed state).

Further, an actuator holder 531 for fixing the actuator 33 and a lens holder 532 for fixing the second lens 34 are provided on the upper base 53.

Preferably, when the analyte is a solution after chemiluminescence immune reaction, the excitation light emitted from the exciter 33 is used to excite the analyte twice to generate two chemiluminescence signals, and the detector 43 records the readings of the two chemiluminescence signals. After the two readings are finished, the two readings are respectively processed, and when the amplification of the second reading and the first reading is larger than the maximum value of the standard curve, whether Hook risk exists in the immunoassay can be judged. According to the two readings of chemiluminescence, the difference between the second reading and the first reading is amplified to be A, and a standard curve is respectively made according to the first reading of a series of known standard substances containing target antigens (or antibodies) to be detected and the amplification A of the two readings; the concentration of the test substance can be determined by comparing the first reading of the test substance containing the target antigen (or antibody) to be tested and the amplification A of the two readings with the standard curve.

In addition, because the first switch 32 for controlling the excitation light path 31 and the second switch 42 for controlling the signal light path 41 are linked in the opposite direction, the detection process of the reactant in the reaction cup excited by the excitation light and the reactant luminescent signal can not be interfered with each other, thereby improving the accuracy of the detection information of the reactant luminescent signal and shortening the detection period.

The incubation apparatus 1 of the present invention will be described in detail below.

As shown in fig. 1 and 2, the incubation device 1 includes a reagent chamber 6 for accommodating the reaction cup 62 and a rotating member 7 for rotating the reagent chamber 6, and the detection mechanism 2 is fixed at a detection position on the reagent chamber 6.

The fixing device 61 is arranged in the reagent bin 6, the reaction cup 62 is arranged on the fixing device 61, and the fixing device 61 rotates along with the reagent bin 6.

The fixing device 61 is configured in a disk shape, fixing grooves 63 are formed at equal intervals on the circumference of the fixing device 61, and the cuvettes 62 are disposed in the fixing grooves 63.

Further, in order to keep the cuvette 62 stable during the rotation following the reagent cartridge 6, a stopper may be provided in each of the fixing grooves 63, respectively.

Alternatively, the stopper is an elastic protrusion provided on an inner sidewall of the fixing groove 63, and the elastic protrusion is compressed when the reaction cups 62 are inserted into the corresponding fixing grooves 63, respectively, so as to fix the positions of the reaction cups 62.

As shown in fig. 1, an opening is provided on a sidewall of the reagent cartridge 6, through which the cuvette 62 is inserted into the fixing groove 63.

The rotating member 7 includes a supporting body 71, and a rotating shaft 72 and a motor 73 respectively provided on the supporting body 71, the motor 73 is connected to the rotating shaft 72 through a timing belt 74, and the rotating shaft 72 is connected to a rotation connecting portion at the bottom of the reagent cartridge 6.

Specifically, the support body 71 is a flat plate, and a large synchronizing wheel and a small synchronizing wheel connected to a timing belt 74 and the timing belt 74 are respectively provided on the upper portion of the support body 71, wherein the large synchronizing wheel is connected to the rotary shaft 72, and the small synchronizing wheel is driven by a motor 73. Of course, the rotating shaft 72 and the motor 73 can also be connected through a gear mechanism and a chain mechanism, which are not described in detail herein.

The support body 71 is provided with four mounting holes 711, and the four mounting holes 711 are provided at four corners of the support body 71, respectively. The mounting hole 711 is provided therein with a support column 75 connected to the bottom of the reagent cartridge 6.

The rotary member 7 is provided with a positioning device 8 for reading the position of the reagent cartridge 6.

The positioning device 8 includes a sensor 81 fixed to the support 71 and a sensor flag 82 provided at the bottom of the rotary shaft 72, the sensor flag 82 being flush with the height of the sensor 81 and rotating with the rotary shaft 72.

The initial position of the carousel can be determined by the sensor 81 and the sensor flag 82, and the desired test receptacles can be positioned in the current position by this initial position.

Specifically, the sensor 81 includes a zero position sensor and a position sensor disposed below the zero position sensor, and the sensor light blocking sheet 82 includes a zero position blocking sheet and a code wheel, wherein the zero position blocking sheet and the code wheel are respectively fixed at the upper end and the lower end of the large synchronizing wheel, and the zero position blocking sheet intermittently passes through the zero position sensor when rotating along with the rotating shaft 72, and can be used for calibrating the zero position of rotation; a plurality of grooves are arranged on the coded disc at equal angles, each groove corresponds to one test container, and when the coded disc rotates along with the rotating shaft, different grooves pass through the position sensors respectively, and the position information of the corresponding test containers can be acquired.

While the invention has been described with reference to a preferred embodiment, various modifications may be made and equivalents may be substituted for elements thereof without departing from the scope of the invention. In particular, the technical features mentioned in the embodiments can be combined in any way as long as there is no structural conflict. It is intended that the invention not be limited to the particular embodiments disclosed, but that the invention will include all embodiments falling within the scope of the appended claims.