阅读说明：本技术 一种复合微生物菌剂提高土壤肥力的方法 (Method for improving soil fertility by using compound microbial agent ) 是由 李辉 王廉馨 林丹妮 韩昀穌 纪月 陈俊良 白若彤 于 2019-11-25 设计创作，主要内容包括：本发明涉及土壤修复技术领域,具体涉及一种复合微生物菌剂提高土壤肥力的方法,方法如下：将醋酸菌接入醋酸菌液体培养基中,在30℃、120r/min摇床中培养18h,得到醋酸菌种子液；将假单胞菌接入营养肉汤液体培养基中,在30℃、120r/min摇床中培养18h,得到假单胞菌种子液。按体积比为1:1,将醋酸菌种子液和假单胞菌种子液在30℃、120r/min摇床中共同培养,发酵液培养至第8小时,取0.1ml发酵液分梯度进行平板涂布,并在30℃恒温培养箱中培养48h；发酵液培养至第40小时,取0.1ml发酵液分梯度进行平板涂布,并在30℃恒温培养箱中培养48h,48小时终止发酵；每亩土地喷施500mL复合菌剂的稀释液后种植植物。(The invention relates to the technical field of soil remediation, in particular to a method for improving soil fertility by using a compound microbial agent, which comprises the following steps: inoculating acetic acid bacteria into an acetic acid bacteria liquid culture medium, and culturing for 18h in a shaking table at 30 ℃ and 120r/min to obtain an acetic acid bacteria seed liquid; inoculating Pseudomonas into nutrient broth liquid culture medium, and culturing in shaking table at 30 deg.C and 120r/min for 18h to obtain Pseudomonas seed liquid. Co-culturing acetic acid bacteria seed liquid and pseudomonas seed liquid in a shaking table at 30 ℃ and 120r/min according to the volume ratio of 1:1, culturing fermentation liquor for 8 hours, taking 0.1ml of fermentation liquor to perform plate coating in a gradient manner, and culturing for 48 hours in a constant-temperature incubator at 30 ℃; culturing the fermentation liquid for 40 hours, taking 0.1ml of fermentation liquid for plate coating in a gradient manner, culturing for 48 hours in a constant-temperature incubator at 30 ℃, and stopping fermentation for 48 hours; and (3) spraying 500mL of the diluent of the compound microbial inoculum on each mu of land, and then planting the plants.)

1. A method for improving soil fertility by using a compound microbial agent is characterized by comprising the following steps:

1) culturing acetic acid bacteria seed liquid: inoculating acetic acid bacteria into an acetic acid bacteria liquid culture medium, and culturing for 18h in a shaking table at 30 ℃ and 120r/min to obtain an acetic acid bacteria seed liquid;

2) culturing a pseudomonas seed solution: inoculating pseudomonas into a nutrient broth liquid culture medium, and culturing for 18h in a shaking table at 30 ℃ and 120r/min to obtain pseudomonas seed liquid;

3) culturing the compound microbial agent: culturing acetic acid bacteria seed liquid and pseudomonas seed liquid in a shaking table at 30 ℃ and 120r/min according to the volume ratio of 1:1, culturing fermentation liquor for 8 hours, taking 0.1ml of fermentation liquor to perform plate coating in a gradient manner in an acetic acid bacteria solid culture medium and a nutrient broth solid culture medium, and culturing for 48 hours in a 30 ℃ constant-temperature incubator; culturing the fermentation liquid for 40 h, performing plate coating on 0.1ml of fermentation liquid in a gradient manner in an acetic acid bacteria solid culture medium and a nutrient broth solid culture medium, culturing for 48h in a constant-temperature incubator at 30 ℃, and stopping fermentation for 48 h;

4) spraying or drip irrigation: and (3) spraying 500mL of the diluent of the compound microbial inoculum on each mu of land, and then planting the plants.

2. The method for improving soil fertility by using compound microbial inoculant according to claim 1, wherein the acetic acid bacteria culture medium in step 1) is: 10-20g of yeast extract, 10-20g of glucose, 20-30g of calcium carbonate and 1000ml of distilled water, and adjusting the pH value to 6.8.

3. The method for improving soil fertility by using the compound microbial inoculant according to claim 1, wherein in the step 1), the mass ratio of acetic acid bacteria: acetic acid bacteria liquid culture medium 1: 100-500.

4. The method for improving soil fertility by using compound microbial inoculant according to claim 1, wherein in the step 2), the nutrient broth culture medium: 3-5g of beef extract, 10-20g of peptone, 5-10g of sodium chloride and 1000ml of distilled water, and adjusting the pH value to 7.2-7.4.

5. The method for improving soil fertility by using the compound microbial inoculant according to claim 1, wherein in the step 2), the mass ratio of pseudomonas: nutrient broth liquid medium ═ 1: 100-500.

6. The method for improving soil fertility by using the compound microbial inoculant according to claim 1, wherein in the step 4), the diluent of the compound microbial inoculant is 1000 times as high as the diluent of the compound microbial inoculant.

Technical Field

The invention relates to the technical field of soil remediation, and particularly relates to a method for improving soil fertility by using a compound microbial agent.

Background

The microbial agent is developed according to the soil microecology principle, the plant nutrition principle and the basic concept of modern organic agriculture. The microbial agent is a novel fertilizer biological product which leads crops to obtain required nutrients (fertilizer) through the life activities of microorganisms, and is a fertilizer in agricultural production (also called third-generation fertilizer).

The microbial agent contains efficient active beneficial microorganisms, is suitable for crops, improves the nutrient utilization rate, breaks through the inherent weaknesses of the 'specificity', 'limitation' and 'special fertilizer' of common biological fertilizers, and is incomparable with other biological fertilizers. The soil degradation agent can be suitable for various types of soil and helps to degrade pollutants in soil, water and atmosphere.

In the development of modern agriculture, microbial agents are used more and more widely, and the research on the microbial agents at the present stage is more and more focused. The microbial agent can promote the growth of crops, improve the yield, increase the number and activity of beneficial microorganisms in soil, and improve the soil fertility, water retention and disease resistance.

Disclosure of Invention

In order to achieve the purpose, the invention adopts the technical scheme that: a method for improving soil fertility by using a compound microbial agent comprises the following steps:

1) culturing acetic acid bacteria seed liquid: inoculating acetic acid bacteria into an acetic acid bacteria liquid culture medium, and culturing for 18h in a shaking table at 30 ℃ and 120r/min to obtain an acetic acid bacteria seed liquid;

2) culturing a pseudomonas seed solution: inoculating Pseudomonas into nutrient broth liquid culture medium, and culturing in shaking table at 30 deg.C and 120r/min for 18h to obtain Pseudomonas seed liquid.

3) Culturing the compound microbial agent: culturing acetic acid bacteria seed liquid and pseudomonas seed liquid in a shaking table at 30 ℃ and 120r/min according to the volume ratio of 1:1, culturing fermentation liquor for 8 hours, taking 0.1ml of fermentation liquor to perform plate coating in a gradient manner in an acetic acid bacteria solid culture medium and a nutrient broth solid culture medium, and culturing for 48 hours in a 30 ℃ constant-temperature incubator; culturing the fermentation liquid for 40 h, performing plate coating on 0.1ml of fermentation liquid in a gradient manner in an acetic acid bacteria solid culture medium and a nutrient broth solid culture medium, culturing for 48h in a constant-temperature incubator at 30 ℃, and stopping fermentation for 48 h;

4) spraying or drip irrigation: and (3) spraying 500mL of the diluent of the compound microbial inoculum on each mu of land, and then planting the plants.

Preferably, in the method for improving soil fertility by using the compound microbial agent, in step 1), the acetic acid bacteria culture medium is: 10-20g of yeast extract, 10-20g of glucose, 20-30g of calcium carbonate and 1000ml of distilled water, and adjusting the pH value to 6.8.

Preferably, in the method for improving soil fertility by using the compound microbial agent, in the step 1), the ratio of acetic acid bacteria: acetic acid bacteria culture medium is 1: 100-500.

Preferably, in the method for improving soil fertility by using the compound microbial agent, in step 2), the nutrient broth culture medium: 3-5g of beef extract, 10-20g of peptone, 5-10g of sodium chloride and 1000ml of distilled water, and adjusting the pH value to 7.2-7.4.

Preferably, in the method for improving soil fertility by using the compound microbial agent, in the step 2), the mass ratio of pseudomonas: nutrient broth medium ═ 1: 100-500.

Preferably, in the method for improving soil fertility by using the compound microbial inoculant, in the step 4), the diluent of the compound microbial inoculant is 1000 times of the diluent of the compound microbial inoculant which is diluted by 100 times.

The invention has the beneficial effects that:

1. in the invention, the acetic acid bacteria can directly convert glucose into acetic acid when the sugar source is sufficient, or convert ethanol into acetaldehyde and then into acetic acid when the sugar source is lacking, thus being beneficial to the growth of crops. The pseudomonas can be used for biological control and plant growth promotion, and can also be used for biological disinsection, and the main action of biological control is plant pathogenic fungi, such as fusarium and rhizoctonia solani.

2. High yield increasing effect and high yield increasing rate. And spraying 500ml of diluent of the compound microbial inoculum to the turned field by adopting a spraying or drip irrigation method, wherein the yield of the potatoes is increased by 600 to 1400 jin on average, and the yield is increased by about 20 to 25 percent.

3. The invention is nontoxic, harmless and pollution-free, can effectively improve the soil structure, enhance the physical properties of the soil, obviously improve the soil fertility and reduce the fertilizer consumption. The use of a large amount of microbial agents can also reduce the emission of greenhouse gases, and really achieves no pollution.

4. The soil treated by the method has no fertilizer residue, can effectively improve the soil structure, obviously improve the soil fertility, is suitable for the growth of crops, and improves the yield and the quality of the crops.

Drawings

FIG. 1 is a photograph of potatoes planted with the microbial composite inoculant prepared in example 1.

In the figure, the first row of potatoes are potatoes planted by using the microbial composite inoculant, and the second row of potatoes are potatoes planted without using the microbial composite inoculant.

Fig. 2 is a picture of potatoes planted with the microbial composite inoculant prepared in example 2.

In the figure, the first row of potatoes are potatoes planted by using the microbial composite inoculant, and the second row of potatoes are potatoes planted without using the microbial composite inoculant.

FIG. 3 is a photograph of potatoes planted with the microbial composite inoculant prepared in example 3.

In the figure, the first row of potatoes are potatoes planted by using the microbial composite inoculant, and the second row of potatoes are potatoes planted without using the microbial composite inoculant.

FIG. 4 shows growth curves of Acetobacter xylinum and Pseudomonas bacteria cultured in the respective media at 30 ℃ and 120r/min, respectively.

Detailed Description

The present invention is further illustrated by the following examples.

Materials: the invention selects acetic acid bacteria and pseudomonas screened from soil on the surface of rice with good growth vigor.