阅读说明：本技术 一种蔬菜种植用土壤活化植物调控菌剂及其制备方法 (Soil-activated plant regulating microbial inoculum for vegetable planting and preparation method thereof ) 是由 赵桂根 胡德强 方志平 张金龙 王瑞俊 阮阳阳 陈先发 于 2021-07-14 设计创作，主要内容包括：本发明公开了一种蔬菜种植用土壤活化植物调控菌剂及其制备方法,属于植物调控菌剂技术领域,包括由以下质量百分比的组分构成：内生真菌25～35％、菌体活化液45～80％、土壤活化菌10～20％和微量元素0.1～0.2％。该菌剂喷洒于土壤中后不仅能够依据喷洒浓度调节蔬菜生长,而且更易被蔬菜吸收利用；并添加固氮梭菌,捕捉游离的氮元素,提高蔬菜利对空气中氮元素的利用率。(The invention discloses a soil-activated plant regulating microbial inoculum for vegetable planting and a preparation method thereof, belonging to the technical field of plant regulating microbial inoculum and comprising the following components in percentage by mass: 25-35% of endophytic fungi, 45-80% of thallus activating solution, 10-20% of soil activating bacteria and 0.1-0.2% of trace elements. After the microbial inoculum is sprayed in soil, the growth of vegetables can be adjusted according to the spraying concentration, and the microbial inoculum is easier to be absorbed and utilized by the vegetables; and the nitrogen-fixing clostridium is added to capture free nitrogen elements, so that the utilization rate of the nitrogen elements in the air by the vegetables is improved.)

1. A soil-activated plant regulating microbial inoculum for vegetable planting is characterized by comprising the following components in percentage by mass: 25-35% of endophytic fungi, 45-80% of thallus activating solution, 10-20% of soil activating bacteria and 0.1-0.2% of trace elements;

the endophytic fungi are fungi living in stems and leaves of the seedling stage of the planted vegetables, and the endophytic fungi comprise the following components in percentage by mass: 5-20% of epicoccum nigrum, 5-20% of Geobacillus gracilis, 20-80% of penicillium, 5-20% of Acremonium and 5-20% of Clostridium azotoformans.

2. The soil-activated plant regulating microbial inoculum for vegetable planting as claimed in claim 1, which comprises the following components by mass percent: 29 percent of endophytic fungi, 56.2 percent of thallus activating solution, 14.6 percent of soil activating bacteria and 0.2 percent of trace elements.

3. The soil-activated plant regulating microbial inoculum for vegetable planting as claimed in claim 1, wherein the microbial inoculum activating solution comprises the following components in percentage by mass: 2-5% of ammonia nitrogen, 2-5% of nitrate nitrogen and nitrate P₂O₅2-5% of soluble potassium carbonate K₂O2-5%, humic acid 2-5%, gum sugar 55-72%, and the balance of water.

4. The soil-activated plant regulating microbial inoculum for vegetable planting as claimed in claim 1, which is characterized in that the soil-activated microbial inoculum comprises the following components in percentage by mass: 8-12% of clostridium rubrum, 8-12% of acetobacter gluconicum, 8-12% of pseudomonas fluorescens, 8-12% of lactobacillus paracasei, 8-12% of lactobacillus pentosus, 8-12% of lactobacillus, 8-12% of bacillus, 8-12% of lactobacillus plantarum and 8-12% of acinetobacter jejuni.

5. The soil-activating plant-regulating bacterial agent for vegetable cultivation as claimed in claim 1, wherein said trace element is selected from any one or a mixture of at least two of B, Zn, Mn, Cu and Co.

6. The soil-activated plant regulating microbial inoculum for vegetable planting according to claim 1, wherein the endophytic fungi mainly comprises the following components in percentage by mass: 5% of epicoccum nigrum, 5% of Geosporium gracilis, 20% of penicillium, 5% of Acremonium and 5% of Clostridium azocum.

7. The soil-activated plant regulating microbial inoculum for vegetable planting according to claim 1, wherein the endophytic fungi mainly comprises the following components in percentage by mass: 5% of epicoccum nigrum, 5% of Geosporium gracilis, 40% of penicillium, 5% of Acremonium and 5% of Clostridium azocum.

8. The soil-activated plant regulating microbial inoculum for vegetable planting according to claim 1, wherein the endophytic fungi mainly comprises the following components in percentage by mass: 5% of epicoccum nigrum, 5% of Geosporium gracilis, 60% of penicillium, 5% of Acremonium and 5% of Clostridium azocum.

9. The soil-activated plant regulating microbial inoculum for vegetable planting according to claim 1, wherein the endophytic fungi mainly comprises the following components in percentage by mass: 5% of epicoccum nigrum, 5% of Geosporium gracilis, 80% of penicillium, 5% of Acremonium and 5% of Clostridium azocum.

10. A method for preparing a soil-activating plant-regulating microbial inoculum for vegetable planting according to any one of claims 1 to 9, comprising the steps of:

s101: preparing ammonia nitrogen, nitrate, soluble potassium carbonate, humic acid, gum sugar, water and trace elements into activating solution according to a certain proportion;

s102: soil activating bacteria, namely selecting red clostridium, gluconacetobacter, pseudomonas fluorescens, lactobacillus paracasei, lactobacillus pentosus, lactobacillus sporogenes, lactobacillus plantarum and acinetobacter jejuni from a strain bank, respectively culturing, and putting a certain number of colonies into an activating solution;

s103: extracting corresponding strains of epiphytic coccus nigricans, alternaria tenuis, penicillium, acremonium and clostridium azotoformans from vegetable stalks, sprouts and fruits, respectively culturing, taking a certain number of colonies, and putting into an activating solution to obtain the soil activating plant regulating microbial inoculum.

Technical Field

The invention relates to the technical field of plant regulating and controlling microbial inoculum, in particular to a soil-activated plant regulating and controlling microbial inoculum for vegetable planting and a preparation method thereof.

Background

The use of chemical pesticide and inorganic fertilizer can effectively promote the yield increase of crops, and plays an important role in the rapid development of agriculture in China. However, due to the long-term application of a large amount of inorganic fertilizers and chemical pesticides, the quality of agricultural products in China is reduced on one hand; on the other hand, due to the long-term use of inorganic fertilizers and chemical pesticides in large quantities, soil hardening, salinization and more severe drug resistance of plant diseases and insect pests are caused, and a series of problems of food safety, environmental pollution and the like are brought. Therefore, biological pesticides are advocated to prevent and treat plant diseases and insect pests, organic fertilizers are applied to improve soil, resistance of the plant diseases and insect pests is relieved to a certain extent, and organic matter content in the soil is increased.

Patent No. CN202011526329.3 discloses a method for promoting crop growth and disease resistance by using multiple bactericides in a synergistic manner, belonging to the field of agricultural planting. Different microbial agents are used in different growth periods of crops, and firstly, the microbial agent containing bacillus subtilis and a base fertilizer are applied simultaneously; secondly, the paenibacillus polymyxa suspending agent is used for dipping roots when the paenibacillus polymyxa is used for seed dressing during sowing or transplanting, and the paenibacillus polymyxa microbial inoculum is used for flushing after seedlings emerge neatly or seedlings are transplanted and slowed; and finally spraying the bacillus amyloliquefaciens on the overground part of the crop. The microbial inoculum has the advantages of multiple strains, high preparation difficulty, great difficulty in regulating and controlling the growth of crops, slow synergism, long time for synergism, and unobvious effect, and can not achieve the purpose of manual intervention and control basically, so that the purpose of regulating and controlling the growth of plants can not be achieved.

Disclosure of Invention

The invention aims to provide a soil-activated plant regulating microbial inoculum for vegetable planting and a preparation method thereof, wherein the microbial inoculum can regulate the growth of vegetables according to the spraying concentration after being sprayed in soil, and is easier to be absorbed and utilized by the vegetables; and the nitrogen-fixing clostridium is added to capture free nitrogen elements and improve the utilization rate of the nitrogen elements in the air by the vegetables so as to solve the problems in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme: a soil-activated plant regulating microbial inoculum for vegetable planting comprises the following components in percentage by mass: 25-35% of endophytic fungi, 45-80% of thallus activating solution, 10-20% of soil activating bacteria and 0.1-0.2% of trace elements;

the endophytic fungi are fungi living in stems and leaves of the seedling stage of the planted vegetables, and the endophytic fungi comprise the following components in percentage by mass: 5-20% of epicoccum nigrum, 5-20% of Geobacillus gracilis, 20-80% of penicillium, 5-20% of Acremonium and 5-20% of Clostridium azotoformans.

Further, the paint comprises the following components in percentage by mass: 29 percent of endophytic fungi, 56.2 percent of thallus activating solution, 14.6 percent of soil activating bacteria and 0.2 percent of trace elements.

Further, the thallus activating solution comprises the following components in percentage by mass: 2-5% of ammonia nitrogen, 2-5% of nitrate nitrogen and nitrate P₂O₅2-5% of soluble potassium carbonate K₂O2-5%, humic acid 2-5%, gum sugar 55-72%, and the balance of water.

Further, the soil activating bacteria comprise the following components in percentage by mass: 8-12% of clostridium rubrum, 8-12% of acetobacter gluconicum, 8-12% of pseudomonas fluorescens, 8-12% of lactobacillus paracasei, 8-12% of lactobacillus pentosus, 8-12% of lactobacillus, 8-12% of bacillus, 8-12% of lactobacillus plantarum and 8-12% of acinetobacter jejuni.

Further, the trace elements are selected from any one or a mixture of at least two of B, Zn, Mn, Cu and Co.

Further, the endophytic fungi mainly comprise the following components in percentage by mass: 5% of epicoccum nigrum, 5% of Geobacillus gracilis, 20% of penicillium, 5% of Acremonium, 5% of Clostridium azotobacter and the balance of water.

Further, the endophytic fungi mainly comprise the following components in percentage by mass: 5% of epicoccum nigrum, 5% of Geobacillus gracilis, 40% of penicillium, 5% of Acremonium, 5% of Clostridium azotobacter and the balance of water.

Further, the endophytic fungi mainly comprise the following components in percentage by mass: 5% of epicoccum nigrum, 5% of Geobacillus gracilis, 60% of penicillium, 5% of Acremonium, 5% of Clostridium azotobacter and the balance of water.

Further, the endophytic fungi mainly comprise the following components in percentage by mass: 5% of epicoccum nigrum, 5% of Geobacillus gracilis, 80% of penicillium, 5% of Acremonium, 5% of Clostridium azotobacter and the balance of water.

According to another aspect of the invention, a preparation method of a soil-activated plant regulating microbial inoculum for vegetable planting is provided, which comprises the following steps:

s101: preparing a thallus activating solution, namely preparing ammonia nitrogen, nitrate, soluble potassium carbonate, humic acid, gum sugar, water and trace elements into the activating solution according to a certain proportion;

s102: preparing soil activating bacteria, selecting red clostridium, gluconacetobacter, pseudomonas fluorescens, lactobacillus paracasei, lactobacillus pentosus, lactobacillus plantarum and acinetobacter jejuni from a strain bank, respectively culturing, and putting a certain number of colonies into an activating solution;

s103: preparing a microbial inoculum, extracting corresponding strains of epiphytic coccus, alternaria tenuissima, penicillium, acremonium and clostridium azotoformans from vegetable stalks, sprouts and fruits, respectively culturing and taking a certain number of colonies to be put into an activating solution to obtain the soil activating plant regulating microbial inoculum.

Compared with the prior art, the invention has the beneficial effects that:

the penicillium contains a certain amount of penicillin, the penicillin has the effects of promoting the growth of vegetables at low concentration and inhibiting the growth of the vegetables at high concentration, the penicillium is endophytic fungi extracted from the vegetables, and after the fungicide is sprayed in the soil, the growth of the vegetables can be regulated according to the spraying concentration, and the fungicide is more easily absorbed and utilized by the vegetables; and the nitrogen-fixing clostridium is added to capture free nitrogen elements, so that the utilization rate of the nitrogen elements in the air by the vegetables is improved.

Drawings

FIG. 1 is a flow chart of a preparation method of the soil-activated plant regulating microbial inoculum for vegetable planting.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example one

A soil-activated plant regulating microbial inoculum for vegetable planting comprises the following components in percentage by mass: 29 percent of endophytic fungi, 56.2 percent of thallus activating solution, 14.6 percent of soil activating bacteria and 0.2 percent of trace elements;

the endophytic fungi are fungi living in stems and leaves of the seedling stage of the planted vegetables, and the endophytic fungi comprise the following components in percentage by mass: 5% of epicoccum nigrum, 5% of Geobacillus gracilis, 20% of penicillium, 5% of Acremonium ramosum, 5% of Clostridium azotobacum and the balance of water;

the thallus activating solution comprises the following components in percentage by mass: 4% of ammonia nitrogen, 4% of nitrate nitrogen and 4% of nitrate P₂O₅4% soluble potassium carbonate K₂O4%, humic acid 4%, gum sugar 62% and the balance of water;

the soil activating bacteria comprise the following components in percentage by mass: 10% of clostridium rubrum, 10% of acetobacter gluconicum, 10% of pseudomonas fluorescens, 10% of lactobacillus paracasei, 10% of lactobacillus pentosus, 10% of lactobacillus sporogenes, 10% of lactobacillus plantarum and 10% of acinetobacter jejuni;

the microelements are selected from equal mass mixtures of B, Zn, Mn, Cu and Co.

Referring to fig. 1, in order to better show the preparation process of the soil-activated plant-regulating microbial inoculum for vegetable planting, the embodiment now provides a preparation method of the soil-activated plant-regulating microbial inoculum for vegetable planting, which includes the following steps:

s101: preparing a thallus activating solution, namely preparing ammonia nitrogen, nitrate, soluble potassium carbonate, humic acid, gum sugar, water and trace elements into the activating solution according to a certain proportion;

s102: preparing soil activating bacteria, selecting red clostridium, gluconacetobacter, pseudomonas fluorescens, lactobacillus paracasei, lactobacillus pentosus, lactobacillus plantarum and acinetobacter jejuni from a strain bank, respectively culturing, and putting a certain number of colonies into an activating solution;

s103: preparing a microbial inoculum, extracting strains of epiphytic coccus, alternaria tenuissima, penicillium, acremonium and clostridium azotoformans corresponding to vegetable stalks, sprouts and fruits, respectively culturing and taking a certain number of bacterial colonies and putting the bacterial colonies into an activating solution to obtain a soil activating plant regulating microbial inoculum, selecting a deficient strain from a strain library, taking pepper as an example, selecting leaves, stalks and fruits of a healthy pepper plant with good growth, respectively sampling in a germination period, a seedling period, a flowering and fruit setting period and a fruiting period, then obtaining the strain by adopting a separation prevention method, namely washing the sample twice by distilled water, then soaking the sample in 70% alcohol for 1 minute and 3% sodium hypochlorite for 4 minutes, then soaking in 70% alcohol for 30s, washing in sterilized water for 3 times, washing for one minute each time, cutting the tissue after surface disinfection into tissue blocks with the size of 5-7 mm, transferring the tissue blocks to a culture medium under the aseptic operation, adding 100ug/ml oxytetracycline before pouring to inhibit the growth of bacteria to obtain fungus bacterial liquid, coating for several times by coating method to obtain multiple fungus colonies, and sequentially identifying and selecting out required strains.

Example two

The difference between the present embodiment and the first embodiment is only that the mass ratios of the components of the endophytic fungi in the present embodiment are different, and the endophytic fungi in the present embodiment mainly comprises the following components in mass percentage: 5% of epicoccum nigrum, 5% of Geobacillus gracilis, 40% of penicillium, 5% of Acremonium, 5% of Clostridium azotobacter and the balance of water.

EXAMPLE III

The difference between the present embodiment and the first embodiment is only that the mass ratios of the components of the endophytic fungi in the present embodiment are different, and the endophytic fungi in the present embodiment mainly comprises the following components in mass percentage: 5% of epicoccum nigrum, 5% of Geobacillus gracilis, 60% of penicillium, 5% of Acremonium, 5% of Clostridium azotobacter and the balance of water.

Example four

The difference between this embodiment and the first embodiment is only that the mass ratios of the components of the endophytic fungi in this embodiment are different, and the endophytic fungi in this embodiment mainly comprises the following components in mass percentage: 5% of epicoccum nigrum, 5% of Geosporium gracilis, 80% of penicillium, 5% of Acremonium and 5% of Clostridium azocum.

Comparative example

The difference between the comparative example and the first example is that the endophytic fungi in the comparative example does not contain penicillium and the mass ratio of the components is different, and the endophytic fungi in the comparative example mainly comprises the following components in percentage by mass: 5% of epicoccum nigricans, 5% of Geobacillus gracilis, 5% of Acremonium cladosporium, 5% of Clostridium azotobacter and the balance of water.

Statistics is carried out on the strain types and the mass ratios of the components of the strain types in the endophytic fungi of the soil activating plant regulating and controlling microbial inoculum in the above examples and comparative examples, and the obtained data are shown in the following table 1:

TABLE 1 statistic data of strain species and component mass ratio in endophytic fungi

Selecting 500 mu of adjacent vegetable fields with the same area, the same planting density, the same planting and cultivating process and the same planted vegetables which are all peppers in the same product, adopting the soil activated plant regulating and controlling microbial inoculum in the first to fourth examples and the comparative example, spraying the soil activated plant regulating and controlling microbial inoculum in each example to the pepper seed stage, the pepper seedling stage, the flowering and fruit setting stage and the fruiting stage at 100 ml/mu, spraying twice in each cycle, and calculating the cycle T1 from the seed planting day to the germination day, the cycle T2 from the germination day to the flowering day, the cycle T3 from the flowering day to the fruiting day and the cycle T4 from the fruiting day to the fruit shedding day. The statistical measurement result data are shown in the following table 2:

TABLE 2 influence of soil activating plant regulating microbial inoculum on growth cycle of Capsicum annuum

According to the detection data in the first to fourth examples and the comparative example, the endophytic fungi mainly comprise the following components in percentage by mass: 15% of epicoccum nigrum, 15% of Geosporium gracilis, 40% of penicillium, 15% of Acremonium and 15% of Clostridium azotoformans, the growth period of the pepper is shortest, particularly the growth time of the pepper seedling is shortened by 11 days and 7 days compared with that of the pepper seedling without spraying penicillium in the comparative example, and the high-concentration soil-activated plant regulating and controlling microbial inoculum can inhibit the growth of the pepper seedlings and prolong the growth period of the pepper seedlings.

In summary, the following steps: the penicillium contains a certain amount of penicillin, the penicillin has the effects of promoting the growth of vegetables at low concentration and inhibiting the growth of the vegetables at high concentration, the penicillium is endophytic fungi extracted from the vegetables, and after the fungicide is sprayed in the soil, the growth of the vegetables can be regulated according to the spraying concentration, and the fungicide is more easily absorbed and utilized by the vegetables; and the nitrogen-fixing clostridium is added to capture free nitrogen elements, so that the utilization rate of the nitrogen elements in the air by the vegetables is improved.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to cover the technical solutions and the inventive concepts of the present invention within the technical scope of the present invention.