阅读说明：本技术 一种智能化集菌系统 (Intelligent bacteria collecting system ) 是由 黄雪峰 李盛姬 龚友平 徐江荣 于 2021-06-29 设计创作，主要内容包括：本发明涉及一种智能化集菌系统。本发明开瓶模块和蠕动泵模块相对设置,在开瓶模块和蠕动泵模块中间设置有导轨,托盘模块放置在导轨上；机械手模块、蠕动泵模块固定在输液操作平台上。控制输液模块放置在托盘模块上,能够通过机械手模块移动至输液操作平台上并固定。本发明代替了传统的人工集菌过程,使集菌过程更加快速,同时降低了因人工检测操作过程中的可能存在的二次污染,提高了集菌检测的准确性,提高了工作效率。自动化程度高,在集菌过程中,无需人工操作,完成整个集菌的过程。适应性程度高,能够实现多种试液瓶样品的集菌检测。(The invention relates to an intelligent bacterium collecting system. The bottle opening module and the peristaltic pump module are arranged oppositely, a guide rail is arranged between the bottle opening module and the peristaltic pump module, and the tray module is placed on the guide rail; the manipulator module and the peristaltic pump module are fixed on the infusion operation platform. The control infusion module is placed on the tray module, and can be moved to the infusion operation platform through the manipulator module and fixed. The invention replaces the traditional manual bacteria collection process, so that the bacteria collection process is faster, the possible secondary pollution in the manual detection operation process is reduced, the bacteria collection detection accuracy is improved, and the working efficiency is improved. The automation degree is high, and the whole bacteria collecting process is completed without manual operation in the bacteria collecting process. The adaptability degree is high, and the bacteria collection detection of various test solution bottle samples can be realized.)

1. The utility model provides an intelligent collection fungus system which characterized in that: comprises a guide rail, a tray module, a manipulator module, a peristaltic pump module, a turnover mechanism module and a control transfusion module; the tray module is placed on the guide rail, a peristaltic pump module is arranged on one side of the tray module, and a tray positioning device is arranged on the other side of the tray module; the manipulator module and the peristaltic pump module are fixed on the transfusion operation platform; a liquid outlet device is arranged on the transfusion operation platform; the control infusion module is placed on the tray module, and is moved to the infusion operation platform through the manipulator module and fixed;

the tray module comprises a tray, a moistening membrane cleaning liquid bottle, a culture medium bottle, a test article, a container adapting conversion piece and a control infusion module arranged on the tray; the tray is used for placing other articles, and the film moistening and cleaning liquid bottle is used for controlling the infusion module to clean the culture bottle; the container adapting conversion piece is used for placing a culture medium bottle, so that the turnover mechanism can grab the culture medium bottle conveniently; the control infusion module is used for infusion and bacterium collection of the culture bottle;

the control infusion module comprises a first hose mounting assembly, a first needle, a fixed supporting plate, a fixed pressure pipe seat, a horizontal clamp, an incubator tool, a pipe clamping clamp, a movable blade knife and a culture bottle; the fixed supporting plate plays a role in fixing and supporting all the components; the first hose installation component comprises a hose clamp and a single/multiple infusion tube, the single/multiple infusion tube is fixed on the hose clamp, one end of each infusion tube is connected with the first needle through a pipeline, and the other end of each infusion tube is connected with a liquid inlet of the culture bottle through a horizontal clamp, a tube clamping clamp and a movable blade knife in sequence; the culture bottle is placed in the incubator tool, the top of the incubator tool is provided with a cap plug fixing component, a pipe clamping clamp and a movable blade cutter, and a fixed supporting plate at the bottom of the incubator tool is provided with a lower bottle pipeline stop component; one side of the movable blade knife is connected with a tension spring; the top of the culture bottle is provided with a liquid conveying pipe mounting hole and an upper cap plug, the bottom of the culture bottle is provided with a lower bottle pipeline, and the interior of the culture bottle is provided with a filter screen; the liquid in the culture bottle is discharged to a liquid outlet device through a pipe below the bottle; the cap plug fixing component is arranged opposite to the upper cap plug and can drive the upper cap plug to vertically move relative to the incubator tool; the lower bottle pipeline and the lower cap plug in the lower bottle pipeline stop assembly are arranged oppositely, and the lower bottle pipeline stop assembly can drive the lower cap plug to vertically move;

the peristaltic pump module comprises a first peristaltic pump and a second peristaltic pump, and each peristaltic pump is provided with a hose mounting assembly mounting groove; the turnover mechanism module comprises a first turnover mechanism and a second turnover mechanism; the first turnover mechanism is arranged on one side of the first peristaltic pump, and the second turnover mechanism is arranged on one side of the second peristaltic pump; the first turnover mechanism and the first peristaltic pump as well as the second turnover mechanism and the second peristaltic pump are respectively arranged at two sides of the manipulator module;

the first bottle simulating head is arranged on the container adaptive conversion piece, the second bottle simulating head is arranged on the first hose mounting assembly, the third bottle simulating head is arranged on the first needle, the fourth bottle simulating head is arranged on the fixing pipe seat, the fifth bottle simulating head is arranged on the cap plug fixing assembly, and the sixth bottle simulating head is arranged on the under-bottle pipeline stop assembly.

2. The intelligent bacteria collection system of claim 1, wherein: the bottle opening module is arranged opposite to the peristaltic pump module; the tray module further comprises a transition bottle, a multi-needle head tool, a blanking groove, a second needle head and a second hose mounting assembly, the second hose mounting assembly comprises a hose clamp and a single/multiple infusion tube, the single/multiple infusion tube is fixed on the hose clamp, one end of each infusion tube is connected with the second needle head through a pipeline, and the other end of each infusion tube is connected with a liquid outlet of the multi-needle head tool; the number of the liquid conveying pipes is the same as that of the liquid outlets of the multi-needle head tool, and a seventh bottle imitating head is arranged on the multi-needle head tool; the bottle opening module is internally provided with a needle head mounting plate, and the charging chute is used for placing bottle head waste materials generated after the bottle opening module opens bottles.

3. The intelligent bacteria collection system of claim 2, wherein: the transition bottle is filled with a dissolving solution.

4. The intelligent bacteria collection system of claim 2, wherein: the bottle opening module comprises a linear module, a bottle opening lifting cylinder, a liquid pumping lifting cylinder, a bottle head nesting plate, a breaking plate, a top cover cylinder, a bottle opening breaking cylinder, a needle head mounting plate and a top pin cylinder; the bottle opening lifting cylinder and the liquid pumping lifting cylinder are connected with the linear module and horizontally move under the driving of the linear module; the bottle opening lifting cylinder is connected with the bottle head nesting plate and drives the bottle head nesting plate to move vertically; a top cover plate is arranged above the bottle head nesting plate, and the top cover plate is connected with a top cover cylinder and moves horizontally under the driving of the top cover cylinder; the top cover plate and the top cover cylinder are both arranged on the bottle opening lifting cylinder and move along with the bottle head nesting plate; the bottle head nesting plate is connected with the bottle opening breaking cylinder through a breaking plate and is driven by the bottle opening breaking cylinder to move horizontally; the liquid pumping lifting cylinder is connected with the needle head mounting plate and drives the needle head mounting plate to vertically move; the needle head mounting plate is provided with a knock pin cylinder and a clamping groove which are matched with a mounting hole of the multi-needle head tool to complete the fixation of the multi-needle head tool; the bottle head nesting plate is provided with a bottle head nesting hole, and a rubber ring is arranged in the bottle head nesting hole.

5. The intelligent bacteria collection system of claim 2, wherein: the bottle opening module comprises a machine body, a linear module, a bottle opening device, a material pouring device and a liquid pumping device, wherein the linear module, the bottle opening device, the material pouring device and the liquid pumping device are all arranged on the machine body; the linear module is arranged at the top of the machine body and is connected with the bottle opening device, the material pouring device and the liquid pumping device through a module adapter plate; the module adapter plate is connected with the connecting plate on the linear module; a bottle opening device and a material pouring device are arranged on one side of the module adapter plate, and a liquid pumping device is arranged on the other side of the module adapter plate; the bottle opening device comprises a first push rod motor, a slope pressing and breaking plate and a spring push rod, wherein the first push rod motor is fixed on one side of the module adapter plate, and an output shaft of the first push rod motor is fixedly provided with the slope pressing and breaking plate; the inclined plane pressure breaking plate is driven by a first push rod motor to vertically move; when opening the bottle, the discharging side of the bottle opening device is arranged opposite to the feeding side of the pouring device; the liquid pumping device comprises a second push rod motor, a middle connecting plate, a suction needle head adapter and a needle head mounting plate, wherein the second push rod motor is arranged on the other side of the module adapter plate; the output shaft of the second push rod motor is fixedly provided with a middle connecting plate, the middle connecting plate is connected with the suction needle head adapter, and the needle head mounting plate is connected with the suction needle head adapter; the needle head mounting plate is used for fixing a multi-needle head tool.

6. The intelligent bacteria collection system according to any one of claims 1, 2, 4 and 5, wherein: and tray supporting rods are arranged at four corners of the tray and used for stacking a plurality of trays.

7. The intelligent bacteria collection system of claim 1, wherein: the horizontal clamp, the incubator tool, the tube clamping clamp, the movable blade knife and the culture bottle are the same in number and are in one-to-one correspondence; the number of the first hose mounting component is consistent with the number of the infusion tubes in the first hose mounting component.

8. The intelligent bacteria collection system of claim 1, wherein: the incubator tool comprises a first incubator tool, a second incubator tool, a third incubator tool and a fourth incubator tool, and a cap plug fixing assembly, a pipe clamping clamp and a movable blade cutter are arranged at the top of the incubator tool; the first incubator tool and the second incubator tool are fixed on the fixed supporting plate, the left side and the right side of the culture bottle are fixed, and the cap plug fixing assembly is limited; the third incubator tool is arranged at the middle upper parts of the first incubator tool and the second incubator tool and used for fixing the top of the culture bottle; meanwhile, the limiting and fixing functions of the pipe clamping and the movable blade knife are also realized; the fourth incubator tool is arranged in the middle of the first incubator tool and the second incubator tool and used for fixing the middle of the culture bottle; and a bottle lower pipeline stop component is arranged on the fixed supporting plate at the bottom of the incubator tool.

9. The intelligent bacteria collection system according to claim 1 or 8, wherein: the cap plug fixing assembly comprises an upper cap plug clamping plate and an upper cap plug fixing plate, and holes matched with each other are formed between the upper cap plug clamping plate and the upper cap plug fixing plate.

10. The intelligent bacteria collection system according to claim 1 or 8, wherein: the lower pipeline stopping component adopts a lever structure.

11. The intelligent bacteria collection system of claim 1, wherein: the liquid outlet device comprises a liquid outlet diversion trench, a feeding cylinder and a waste liquid recovery trench; the top of the liquid outlet diversion trench is provided with a hole, the interior of the liquid outlet diversion trench is hollow, the bottom of the liquid outlet diversion trench is provided with a liquid outlet, and the liquid outlet is provided with a waste liquid recovery trench; the feeding cylinder is connected with the liquid outlet diversion trench to drive the liquid outlet diversion trench to move back and forth, so that the top opening of the liquid outlet diversion trench is in butt joint with the liquid outlet of the pipeline under the culture bottle.

12. The intelligent bacteria collection system of claim 1, wherein: the tray positioning device comprises an air cylinder and a positioning pin, and the positioning pin is driven by the air cylinder to be matched with the hole on the side wall of the tray, so that positioning is realized.

13. The intelligent bacteria collection system of claim 1, wherein: the mechanical arm module comprises a mechanical arm and a mechanical clamping jaw, and the mechanical clamping jaw comprises a bearing connecting piece, a sliding cylinder and a bottle head holding piece; the bottle head clamping device is connected with the mechanical arm through a bearing connecting piece, and the movement of the sliding cylinder drives the bottle head clamping piece to be opened and closed, so that the bottle head/the imitated bottle head is clamped.

14. The intelligent bacteria collection system of claim 1, wherein: every tilting mechanism all include slip table, slip table cylinder, upset base, the slip table setting is on the upset base, the slip table is connected with the slip table cylinder, at opening and shutting of the drive slip table of slip table cylinder.

Technical Field

The invention belongs to the technical field of intelligentization of sterility test systems, and relates to an intelligent bacterium collecting system for test articles of different test varieties and different packaging specifications.

Background

Sterility test is an important item for qualified product inspection by enterprises and regulatory agencies as a key for quality control of food and drugs, and has definite requirements in the latest 2011 edition of Good Manufacturing Practice (GMP) for drug production, 2015 edition of pharmacopoeia of the people's republic of China (abbreviated as Chinese pharmacopoeia) and United states pharmacopoeia USP31-NF26 sterility test method 71. The sterility test of the test article can be carried out by using a membrane filtration method and a direct inoculation method. Typically, sterility testing of a test article is tested using membrane filtration, and if membrane filtration techniques are not appropriate, direct inoculation of the medium is used. The membrane filtration method generally employs a closed filter comprising a microporous membrane, and the membrane for sterility testing should have a pore size of not more than 0.45 μm, preferably about 50mm in diameter. When the membrane filtration method is used, dilution and washing can be carried out according to the situation, and finally culture is carried out for 14 days by using a culture medium to judge whether the test sample is polluted. Due to the differences of the test products, the dilution amount, the washing amount and the type of the added culture medium are different, so the sterility test device needs to adapt to the changes of the differences of the test products. Typical test articles include: aqueous solution test samples, water-soluble solid test samples, water-insoluble test samples, isopropyl myristate-soluble pharmaceutical and viscous oil test samples, sterile aerosol (spray) test samples, drug-containing syringe test samples, catheter-equipped medical instrument (blood transfusion, infusion bag, etc.) test samples, etc. Meanwhile, the inspection quantity and inspection amount of the test sample are also specified clearly.

Currently, the bacteria collecting operation related to the sterility test depends on manual operation, the automation, integration and intelligence levels are low, and the sterility test efficiency is low; the key processes of bacteria collection operation, cleaning and the like are influenced by personnel, and the significant difference between the sterile inspection result and the efficiency can be brought by the operation of different personnel. In the existing sterility test process, the problems of multiple sample types, multiple-specification sample packaging, multiple-process analysis, discontinuous operation and low bacteria collection analysis efficiency are faced, so that the problems need to be solved by inventing an intelligent bacteria collection operation system and method.

Disclosure of Invention

The invention aims to provide an intelligent bacterium collecting system.

The invention comprises a guide rail, a tray module, a manipulator module, a peristaltic pump module, a turnover mechanism module and a control transfusion module; the tray module is placed on the guide rail, a peristaltic pump module is arranged on one side of the tray module, and a tray positioning device is arranged on the other side of the tray module; the manipulator module and the peristaltic pump module are fixed on the transfusion operation platform; a liquid outlet device is arranged on the transfusion operation platform; the control infusion module is placed on the tray module, and is moved to the infusion operation platform through the manipulator module and fixed;

the tray module comprises a tray, a moistening membrane cleaning liquid bottle, a culture medium bottle, a test article, a container adapting conversion piece and a control infusion module arranged on the tray; the tray is used for placing other articles, and the film moistening and cleaning liquid bottle is used for controlling the infusion module to clean the culture bottle; the container adapting conversion piece is used for placing a culture medium bottle, so that the turnover mechanism can grab the culture medium bottle conveniently; the control infusion module is used for infusion and bacterium collection of the culture bottle;

the control infusion module comprises a first hose mounting assembly, a first needle, a fixed supporting plate, a fixed pressure pipe seat, a horizontal clamp, an incubator tool, a pipe clamping clamp, a movable blade knife and a culture bottle; the fixed supporting plate plays a role in fixing and supporting all the components; the first hose installation component comprises a hose clamp and a single/multiple infusion tube, the single/multiple infusion tube is fixed on the hose clamp, one end of each infusion tube is connected with the first needle through a pipeline, and the other end of each infusion tube is connected with a liquid inlet of the culture bottle through a horizontal clamp, a tube clamping clamp and a movable blade knife in sequence; the culture bottle is placed in the incubator tool, the top of the incubator tool is provided with a cap plug fixing component, a pipe clamping clamp and a movable blade cutter, and a fixed supporting plate at the bottom of the incubator tool is provided with a lower bottle pipeline stop component; one side of the movable blade knife is connected with a tension spring; the top of the culture bottle is provided with a liquid conveying pipe mounting hole and an upper cap plug, the bottom of the culture bottle is provided with a lower bottle pipeline, and the interior of the culture bottle is provided with a filter screen; the liquid in the culture bottle is discharged to a liquid outlet device through a pipe below the bottle; the cap plug fixing component is arranged opposite to the upper cap plug and can drive the upper cap plug to vertically move relative to the incubator tool; the lower bottle pipeline and the lower cap plug in the lower bottle pipeline stop assembly are arranged oppositely, and the lower bottle pipeline stop assembly can drive the lower cap plug to vertically move;

the peristaltic pump module comprises a first peristaltic pump and a second peristaltic pump, and each peristaltic pump is provided with a hose mounting assembly mounting groove; the turnover mechanism module comprises a first turnover mechanism and a second turnover mechanism; the first turnover mechanism is arranged on one side of the first peristaltic pump, and the second turnover mechanism is arranged on one side of the second peristaltic pump; the first turnover mechanism and the first peristaltic pump as well as the second turnover mechanism and the second peristaltic pump are respectively arranged at two sides of the manipulator module;

the first bottle simulating head is arranged on the container adaptive conversion piece, the second bottle simulating head is arranged on the first hose mounting assembly, the third bottle simulating head is arranged on the first needle, the fourth bottle simulating head is arranged on the fixing pipe seat, the fifth bottle simulating head is arranged on the cap plug fixing assembly, and the sixth bottle simulating head is arranged on the under-bottle pipeline stop assembly.

The invention also comprises a bottle opening module which is arranged opposite to the peristaltic pump module; the tray module further comprises a transition bottle, a multi-needle head tool, a blanking groove, a second needle head and a second hose mounting assembly, the second hose mounting assembly comprises a hose clamp and a single/multiple infusion tube, the single/multiple infusion tube is fixed on the hose clamp, one end of each infusion tube is connected with the second needle head through a pipeline, and the other end of each infusion tube is connected with a liquid outlet of the multi-needle head tool; the number of the liquid conveying pipes is the same as that of the liquid outlets of the multi-needle head tool, and a seventh bottle imitating head is arranged on the multi-needle head tool; the bottle opening module is internally provided with a needle head mounting plate, and the charging chute is used for placing bottle head waste materials generated after the bottle opening module opens bottles.

The transition bottle can be filled with a dissolving solution.

The bottle opening module comprises a linear module, a bottle opening lifting cylinder, a liquid pumping lifting cylinder, a bottle head nesting plate, a breaking plate, a top cover cylinder, a bottle opening breaking cylinder, a needle head mounting plate and a top pin cylinder; the bottle opening lifting cylinder and the liquid pumping lifting cylinder are connected with the linear module and horizontally move under the driving of the linear module; the bottle opening lifting cylinder is connected with the bottle head nesting plate and drives the bottle head nesting plate to move vertically; a top cover plate is arranged above the bottle head nesting plate, and the top cover plate is connected with a top cover cylinder and moves horizontally under the driving of the top cover cylinder; the top cover plate and the top cover cylinder are both arranged on the bottle opening lifting cylinder and move along with the bottle head nesting plate; the bottle head nesting plate is connected with the bottle opening breaking cylinder through a breaking plate and is driven by the bottle opening breaking cylinder to move horizontally; the liquid pumping lifting cylinder is connected with the needle head mounting plate and drives the needle head mounting plate to vertically move; the needle head mounting plate is provided with a knock pin cylinder and a clamping groove which are matched with a mounting hole of the multi-needle head tool to complete the fixation of the multi-needle head tool; the bottle head nesting plate is provided with a bottle head nesting hole, and a rubber ring is arranged in the bottle head nesting hole.

The bottle opening module can also comprise a machine body, a linear module, a bottle opening device, a material pouring device and a liquid pumping device, wherein the linear module, the bottle opening device, the material pouring device and the liquid pumping device are all arranged on the machine body; the linear module is arranged at the top of the machine body and is connected with the bottle opening device, the material pouring device and the liquid pumping device through a module adapter plate; the module adapter plate is connected with the connecting plate on the linear module; a bottle opening device and a material pouring device are arranged on one side of the module adapter plate, and a liquid pumping device is arranged on the other side of the module adapter plate; the bottle opening device comprises a first push rod motor, a slope pressing and breaking plate and a spring push rod, wherein the first push rod motor is fixed on one side of the module adapter plate, and an output shaft of the first push rod motor is fixedly provided with the slope pressing and breaking plate; the inclined plane pressure breaking plate is driven by a first push rod motor to vertically move; when opening the bottle, the discharging side of the bottle opening device is arranged opposite to the feeding side of the pouring device; the liquid pumping device comprises a second push rod motor, a middle connecting plate, a suction needle head adapter and a needle head mounting plate, wherein the second push rod motor is arranged on the other side of the module adapter plate; the output shaft of the second push rod motor is fixedly provided with a middle connecting plate, the middle connecting plate is connected with the suction needle head adapter, and the needle head mounting plate is connected with the suction needle head adapter; the needle head mounting plate is used for fixing a multi-needle head tool.

And tray supporting rods are arranged at four corners of the tray and used for stacking a plurality of trays.

The horizontal clamp, the incubator tool, the tube clamping clamp, the movable blade knife and the culture bottle are the same in number and are in one-to-one correspondence; the number of the first hose mounting component is consistent with the number of the infusion tubes in the first hose mounting component.

The incubator tool comprises a first incubator tool, a second incubator tool, a third incubator tool and a fourth incubator tool, and a cap plug fixing assembly, a pipe clamping clamp and a movable blade cutter are arranged at the top of the incubator tool; the first incubator tool and the second incubator tool are fixed on the fixed supporting plate, the left side and the right side of the culture bottle are fixed, and the cap plug fixing assembly is limited; the third incubator tool is arranged at the middle upper parts of the first incubator tool and the second incubator tool and used for fixing the top of the culture bottle; meanwhile, the limiting and fixing functions of the pipe clamping and the movable blade knife are also realized; the fourth incubator tool is arranged in the middle of the first incubator tool and the second incubator tool and used for fixing the middle of the culture bottle; and a bottle lower pipeline stop component is arranged on the fixed supporting plate at the bottom of the incubator tool.

The cap plug fixing assembly comprises an upper cap plug clamping plate and an upper cap plug fixing plate, and holes matched with each other are formed between the upper cap plug clamping plate and the upper cap plug fixing plate.

The lower pipeline stopping component adopts a lever structure.

The liquid outlet device comprises a liquid outlet diversion trench, a feeding cylinder and a waste liquid recovery trench; the top of the liquid outlet diversion trench is provided with a hole, the interior of the liquid outlet diversion trench is hollow, the bottom of the liquid outlet diversion trench is provided with a liquid outlet, and the liquid outlet is provided with a waste liquid recovery trench; the feeding cylinder is connected with the liquid outlet diversion trench to drive the liquid outlet diversion trench to move back and forth, so that the top opening of the liquid outlet diversion trench is in butt joint with the liquid outlet of the pipeline under the culture bottle.

The tray positioning device comprises an air cylinder and a positioning pin, and the positioning pin is driven by the air cylinder to be matched with the hole on the side wall of the tray, so that positioning is realized.

The mechanical arm module comprises a mechanical arm and a mechanical clamping jaw, and the mechanical clamping jaw comprises a bearing connecting piece, a sliding cylinder and a bottle head holding piece; the bottle head clamping device is connected with the mechanical arm through a bearing connecting piece, and the movement of the sliding cylinder drives the bottle head clamping piece to be opened and closed, so that the bottle head/the imitated bottle head is clamped.

Every tilting mechanism all include slip table, slip table cylinder, upset base, the slip table setting is on the upset base, the slip table is connected with the slip table cylinder, at opening and shutting of the drive slip table of slip table cylinder.

The invention replaces the traditional manual bacteria collection process, so that the bacteria collection process is faster, the possible secondary pollution in the manual detection operation process is reduced, the bacteria collection detection accuracy is improved, and the working efficiency is improved. The automation degree is high, manual operation is not needed in the bacteria collecting process, and the operation of the bacteria collecting device is controlled by a Programmable Logic Controller (PLC) and the like, so that the whole bacteria collecting process is completed. The adaptability degree is high, and the bacteria collection detection of a plurality of test solution bottle samples such as ampoule bottles, penicillin bottles and large-volume bottles can be detected. The reliability degree is high: the whole device is carried out under the aseptic condition, so that the external indirect pollution is avoided, and the detection precision is high.

Drawings

FIG. 1 is a schematic view of the entire structure of embodiment 1;

FIG. 2 is a schematic view of the overall structure of the embodiment 1 in operation;

FIG. 3 is a structural schematic of a tray module in embodiment 1;

FIG. 4 is a schematic diagram of a robot module;

FIG. 5 is an enlarged schematic view of the mechanical jaw of FIG. 4;

FIG. 6 is a schematic diagram of a relative position structure of a peristaltic pump module and a turnover module;

FIG. 7 is an enlarged view of the structure of the flipping module;

FIG. 8 is a schematic diagram of a right view of the control infusion module;

FIG. 9 is a schematic view of the structure of the flask of FIG. 8;

FIG. 10 is a rear view of the present invention;

FIG. 11 is a schematic structural view of the cap plug retaining assembly of FIG. 8;

FIG. 12 is a schematic mechanical view of the underbar duct shutoff assembly of FIG. 8;

FIG. 13 is a left side view of the control infusion module;

FIG. 14 is a schematic structural view of the moving blade knife module of FIG. 13;

FIG. 15 is a schematic view of the entire structure of embodiment 2;

FIG. 16 is a schematic view showing the overall structure of the working example 2;

FIG. 17 is a structural diagram of a tray module according to embodiment 2;

FIG. 18 is a schematic structural view of the three-headed needle fixture in FIG. 17;

FIG. 19 is a schematic view showing the overall structure of an uncork module according to embodiment 2;

FIG. 20 is an enlarged partial schematic view of FIG. 19;

FIG. 21 is another enlarged partial schematic view of FIG. 19;

FIG. 22 is a schematic view showing a forward overall structure of another decapping module in example 2;

FIG. 23 is a schematic diagram of a press-break plate structure of the bevel of FIG. 21;

FIG. 24 is a schematic view of the pouring device shown in FIG. 21;

FIG. 25 is an enlarged view of the structure at A in FIG. 23;

FIG. 26 is a schematic view showing a reverse overall structure of another decapping module in example 2;

FIG. 27 is a schematic view of the entire structure of embodiment 3;

FIG. 28 is a schematic view showing the entire structure in operation of embodiment 3;

fig. 29 is a schematic structural view of a tray module in embodiment 3.

Detailed Description

The invention is described in detail below with reference to the accompanying drawings, in which the entire apparatus is operated under sterile conditions.

The test samples were taken in 10ml ampoules, 10ml penicillin bottles, 250ml multi-layer bottles and 100ml aqueous solutions. In the embodiment, each cylinder is provided with a magnetic switch sensor for feeding back the motion state of the cylinder.

Example 1: the test article was contained in a multi-layer large-volume bottle of 250ml and 100ml of aqueous solution.

As shown in fig. 1-2, the intelligent bacteria collecting system in this embodiment includes a guide rail (not shown), a first tray module 100, a manipulator module 400, a peristaltic pump module 500, a turnover mechanism module 600, and a control infusion module 700. The tray module 100 is placed on the guide rail, a peristaltic pump module 500 is arranged on one side of the tray module 100, and a tray positioning device 333 is arranged on the other side of the first tray module 100; the manipulator module 400 and the peristaltic pump module 500 are fixed on the infusion operation platform 344. The control infusion module 700 is placed on the tray module 100, can be moved to the infusion operation platform 344 by the manipulator module 400, and is fixed on the infusion operation platform 344 by the cylinder and the positioning pin.

The transfusion operation platform 344 is provided with a liquid outlet device, and the liquid outlet device comprises a liquid outlet diversion trench 196, a feeding cylinder 197 and a waste liquid recovery trench 198. Three holes are formed in the top of the liquid outlet diversion trench 196 and used for being respectively butted with liquid outlets of lower bottle pipelines of three culture bottles, the middle of the liquid outlet diversion trench is hollow, the gathered liquid flows out from an outlet at the bottom of the liquid outlet diversion trench 196, and a waste liquid recovery trench is arranged right below the outlet and used for recovering waste liquid; the feeding cylinder 197 is connected with the liquid outlet diversion trench for controlling the back and forth movement of the liquid outlet diversion trench 196. The liquid outlet diversion trench 196 and the feeding cylinder 197 are all placed on a bracket with a set height, and the bracket is connected with the control transfusion platform 344 through bolts and fixed at a designated position. When liquid is discharged, the lower cap plug is opened, and the feeding cylinder 197 controls the liquid discharge diversion trench 196 to move forward to the position opposite to the liquid outlet of the pipeline under the culture bottle, so that the waste liquid can flow into the waste liquid recovery trench 198 through the liquid discharge diversion trench 196.

As shown in FIG. 3, the first tray module 100 includes a tray, a bottle of a membrane cleaning solution 111, a bottle of medium 104, a large volume bottled water test article 121, a container adapter 608, and a control infusion module 700 placed on the tray. The container adapting conversion piece 608 is used for placing a small reagent bottle, so that the overturning mechanism can clamp the reagent bottle conveniently; the container adapter 608 is provided with a first dummy head 607, and the infusion control module 700 is provided with a plurality of dummy heads. The manipulator module 400 clamps the dummy heads on the tool module 700, and the infusion control module 700 on the first tray module 100 is movably placed on the infusion operation platform 344 and is fixed on the infusion operation platform 344 through the cylinder and the positioning pin. The tray is provided with holes which cooperate with the tray positioning means 333 to effect positioning of the first tray module 100. In this embodiment, the tray positioning device 333 includes a cylinder and a positioning pin, and the positioning pin is driven by the cylinder to realize positioning.

As shown in fig. 4 and 5, the robot module 400 includes a robot arm and a robot clamping jaw 401, and the robot arm used by the robot module 400 in this embodiment is a four-axis robot arm (e.g., a four-axis robot arm with model number LS6 SCARA). The robotic arm gripper 401 is used in conjunction with the bottle head/dummy bottle head for grasping the bottle head/dummy bottle head of each module, thereby transporting the corresponding module/structure to the designated location. Mechanical gripper 401 includes bearing connection 402, slide cylinder 403, and bottle head hugger 404. The bottle head/dummy head is held tightly by the opening and closing of the bottle head holding member 404 driven by the movement of the sliding cylinder 403 through the connection of the bearing connecting member 402 and the mechanical arm.

As shown in fig. 6, the peristaltic pump module 500 includes two peristaltic pumps (only one is used in this embodiment): a first peristaltic pump 501 and a second peristaltic pump 502 (used in this embodiment), each peristaltic pump having a hose mounting assembly mounting slot 503; the flipping mechanism module 600 includes two flipping mechanisms (only one is used in this embodiment): a first flipping mechanism 601 and a second flipping mechanism 602 (used in this embodiment); the first turnover mechanism 601 is arranged on one side of the first peristaltic pump 501, and the second turnover mechanism 602 is arranged on one side of the second peristaltic pump 502; in use, first canting mechanism 601 is engaged with first peristaltic pump 501 and second canting mechanism 602 (used in this embodiment) is engaged with second peristaltic pump 502.

For convenience of use, the first turnover mechanism 601 and the first peristaltic pump 501, and the second turnover mechanism 602 and the second peristaltic pump 502 are respectively arranged on two sides of the manipulator module.

As shown in fig. 7, each turnover mechanism includes a slide table 605, a slide table cylinder 604, and a turnover base 609. The movement of the sliding table cylinder 604 drives the sliding table 605 to clamp the test solution bottle. The sliding table 605 is arranged on the turning base 609, the sliding table 605 is connected with the sliding table cylinder 604, and the sliding table cylinder 604 drives the sliding table 605 to open and close.

As shown in fig. 8, the control infusion module 700 comprises a first hose mounting assembly 106, a first needle 118, a fixed support plate 777, a fixed pressure pipe seat 701, a horizontal clamp, an incubator fixture, a pipe clamp, a movable blade knife and a culture bottle; the fixing support plate 777 has fixing and supporting functions for all components, and ensures the stability of the whole infusion and collection control device. The horizontal clamp, the incubator tool, the tube clamping clamp, the movable blade knife and the culture bottle are the same in number and are in one-to-one correspondence; the number of which corresponds to the number of infusion tubes in the first hose fitting assembly 106.

The first hose installation component 106 comprises a hose clamp and a single/multiple infusion tube, the single/multiple infusion tube is fixed on the hose clamp, one end of each infusion tube is connected with the first needle 118 through a pipeline, and the other end of the infusion tube is connected with a liquid inlet of the culture bottle through a horizontal clamp, a tube clamping clamp and a movable blade knife in sequence; a second dummy bottle head is arranged on the first hose mounting assembly 106, and a third dummy bottle head is arranged on the first needle 118, so that the manipulator module can grab the first dummy bottle head conveniently.

The fixed pressure pipe seat 701 is used for installing a horizontal clamp and fixing a liquid conveying pipe in the first hose installation assembly 106, so that liquid conveyed in the pipe is accurately and smoothly conveyed into the culture bottle. The horizontal clamp plays a role in opening and closing the infusion tube: when the liquid conveying pipe conveys liquid, the horizontal clamp is opened, and the liquid is conveyed into the culture bottle through the liquid conveying pipe. When the liquid in the culture bottle is fully collected, the horizontal clamp is closed, and the liquid delivery to the culture bottle is stopped. The fourth bottle head 721 is arranged on the fixed pressure pipe seat, so that the mechanical arm can grab the bottle conveniently.

The incubator tool comprises a first incubator tool 714, a second incubator tool 715, a third incubator tool 716 and a fourth incubator tool 717, and a cap plug fixing assembly is arranged at the top of the incubator tool. The first incubator tool 714 and the second incubator tool 715 are fixed on the fixed supporting plate 777, the left side and the right side of the culture bottle are fixed, and the cap plug fixing component is limited; the third incubator tool 716 is arranged at the middle upper part of the first incubator tool 714 and the second incubator tool 715, and is used for fixing the top of the culture bottle; meanwhile, the limiting and fixing functions of the clamp and the movable blade knife are realized, and the later-stage cutting of the infusion tube is facilitated. The fourth incubator assembly 717 is disposed in the middle of the first and second incubator assemblies 714 and 715 for fixing the middle of the culture bottle.

The movable blade knife is used for cutting a pipeline connected between the infusion tube and the culture bottle. The right side of the movable blade knife is connected with a tension spring, the movable blade knife is pushed by the mechanical arm to cut off the infusion tube, and after the infusion tube is cut off, the mechanical arm returns to the original position, and the movable blade knife returns to the original position through the tension of the tension spring.

As shown in fig. 9, the top of the culture bottle is provided with a liquid conveying pipe mounting hole and an upper cap plug, the bottom of the culture bottle is provided with a lower bottle pipeline, a filter screen is arranged inside the culture bottle, and the culture bottle is used for collecting flora existing in an input liquid sample; the culture bottle is placed in the incubator tool, and the incubator tool plays a role in fixing the culture bottle; after the liquid in the culture bottle is discharged into a waste liquid recovery tank 198 of the liquid outlet device through a pipeline under the culture bottle, the flora in the liquid sample conveyed to the culture bottle through a liquid conveying pipe is collected in the culture bottle.

As shown in fig. 10 and 11, a fifth dummy head 702 is disposed on the cap plug fixing assembly, the cap plug fixing assembly includes an upper cap plug clamping plate 703 and an upper cap plug fixing plate 704, and a hole is disposed between the upper cap plug clamping plate 703 and the upper cap plug fixing plate 704 for fixing the upper cap plug of the culture bottle, and the tightness degree of the upper cap plug is adjusted by a tightness adjusting screw fixed on the upper cap plug fixing plate 704. When the fifth dummy head 702 is lifted upward, the upper cap stopper is opened. When the fifth dummy head 702 is pressed downward, the upper cap plug is closed.

As shown in fig. 12, the sixth dummy head 713 plays a role in controlling the lower tube stopping assembly of the culture bottle, and the lower tube stopping assembly adopts a lever structure: when the sixth dummy bottle head 713 is lifted upwards, the under-bottle pipe stop assembly is opened; when the sixth dummy head 713 is pressed downward, the under-bottle conduit stop assembly is closed.

The sixth dummy bottle head 713 is fixedly disposed on a lower bottle pipe stopping assembly, and the lower bottle pipe stopping assembly includes a lower cap plug 705, an optical axis 706, a linear bearing 707, a lever 708, a connecting piece 709, a bracket 710, a front clamp 711, and a rear clamp 712. The lower cap plug 705 is clamped and fixed by a front clamping plate 711 and a rear clamping plate 712; the liquid outlet of the pipeline under the bottle is arranged opposite to the lower cap plug 705; linear bearings 707 are mounted on the front and rear clamping plates, and the linear bearings 707 are connected to the optical axis 706 so that the clamping plates move up and down through the optical axis. The sixth bottle-imitating head 713 is connected with the lever 708, a connecting sheet 709 is arranged below the front clamping plate and the rear clamping plate, the left clamping plate component is connected with the sixth bottle-imitating head 713 on the right side through the connecting sheet, the sixth bottle-imitating head 713 is pressed or pulled to realize that the left clamping plate component moves up and down to drive the lower cap plug 705 to move up and down, and therefore the liquid of the culture bottle is controlled to flow out through the lower cap plug 705.

The tube clamping clip is a switch between the infusion tube and the culture bottle: after the bacteria collection process is completed, the manipulator module 400 presses the tube clamp downwards to close the infusion tube and prevent the mixed bacteria in the air from entering the culture bottle.

As shown in fig. 13, in the present embodiment, three horizontal clamps, three tube clamps and three culture bottles are disposed in the control infusion module 700, and three infusion tubes are disposed in the corresponding first hose installation assembly 106 and are respectively connected to a first pressure tube infusion tube 722, a second pressure tube infusion tube 723 and a third pressure tube infusion tube 724 of the control infusion module 700; the fixed pressure pipe holder 701 is used for mounting a first horizontal clamp 718, a second horizontal clamp 719 and a third horizontal clamp 720, and fixing a first pressure pipe infusion pipe 722, a second pressure pipe infusion pipe 723 and a third pressure pipe infusion pipe 724, so that liquid delivered in the pipes is ensured, and the liquid can be accurately and smoothly delivered to the first culture bottle 731, the second culture bottle 732 and the third culture bottle 733. The first, second, and third infusion lines 722, 723, and 724 ensure that the liquid delivered in the lines (by the peristaltic pump) can be smoothly delivered into the culture flask.

The first flask 731, the second flask 732, and the third flask 733 are all placed in the incubator fixture. The first culture bottle 731 holds the liquid transferred from the first liquid transfer tube 722, and after the liquid in the first culture bottle 731 is discharged through the tube below the bottle, the colonies in the liquid transferred to the first culture bottle 731 through the first liquid transfer tube 722 are collected in the first culture bottle 731. The second culture bottle 732 collects the bacterial flora in the liquid transferred to the second culture bottle 732 through the second transfer tube 723. The bacteria in the liquid transferred to the third flask 733 through the third transfer tube 724 are collected in the third flask 733.

The fifth dummy head 702 functions as a switch for closing and opening the upper caps of the first flask 731, the second flask 732, and the third flask 733. The sixth dummy bottle head 713 functions as a switch for the lower line of the first 731, the lower line of the second 732, and the lower line of the third 733 flask.

As shown in fig. 14, the first clamp 741 is a switch between the first pressure tube 722 and the first flask 731. The second clamp 742 is a switch between the second pinch tube 723 and the second flask 732. The third clamp 743 is a switch between the third pressure tube 724 and the third flask 733.

The first movable blade 751 is used for cutting off the infusion tube connected between the first pressure tube infusion tube 722 and the first culture bottle 731. The second movable blade 752 is used to cut off the infusion tube connected between the second pressure tube infusion tube 723 and the second culture bottle 732. The third movable blade knife 753 is used to cut off the infusion tube connected between the third pressure tube infusion tube 724 and the third culture bottle 733.

The operation process of the embodiment is as follows:

initial state of the control infusion module 700: the upper cap plug blocks the hole at the upper end of the culture bottle, the lower cap plug 705 blocks the liquid outlet at the lower end of the culture bottle, and the three horizontal clamps are in an open state.

Step one, rinsing a culture flask:

the mechanical clamping jaw 401 clamps the fourth dummy bottle head 721 to move the control infusion module 700 on the tray to the control infusion operation platform 344, and fixes the control infusion module 700 on the infusion operation platform 344 through a cylinder and a positioning pin, wherein the liquid outlet device is positioned on one side of a liquid outlet at the lower end of a culture bottle in the control infusion module 700;

then, the manipulator clamping jaws 401 clamp and move the film moistening and cleaning solution bottle 111 on the tray, and the bottle moistening and cleaning solution bottle is placed in a second turnover mechanism 602, and the second turnover mechanism 602 holds the bottle body tightly for positioning;

then, the mechanical gripper 401 grips and moves the first hose mounting assembly 106 into the hose mounting assembly mounting slot 503 of the second peristaltic pump 502;

then, the mechanical clamping jaw 401 lifts the fifth dummy bottle head 702 upwards, and a liquid inlet at the upper end of the culture bottle is opened;

then, the mechanical gripper 401 grips and moves the first needle 118, and inserts the first needle 118 into the bottle 111 of the moisturizing film cleansing liquid. The second turnover mechanism 602 turns over the membrane-wetting cleaning solution bottle 111, opens the second peristaltic pump 502, transfers the membrane-wetting cleaning solution to the culture bottle of the control infusion module 700, wets the microporous filter membrane in the culture bottle, and closes the second peristaltic pump 502 after the membrane-wetting cleaning solution reaches the set injection amount.

Then, the manipulator module 400 pushes the fifth dummy bottle head 702 downwards, and closes the liquid inlet at the upper end of the culture bottle; the manipulator module 400 pulls up the sixth dummy bottle head 713, and opens the liquid outlet at the lower end of the culture bottle; the feeding cylinder 197 is started to drive the liquid outlet diversion trench 196 to horizontally move, so that three holes at the top of the liquid outlet diversion trench 196 are butted with liquid outlet ports (liquid outlet ports of lower pipelines of three culture bottles) at the lower ends of the three culture bottles; the turnover mechanism 602 turns over the upright membrane moistening and cleaning liquid bottle, starts the second peristaltic pump 502, pumps gas into the culture bottle for pressurization, so that the membrane moistening and cleaning liquid is completely discharged from the lower pipelines of the three culture bottle bottles to the waste liquid recovery tank 198 of the liquid discharge device, and closes the peristaltic pump 502 after the membrane moistening and cleaning liquid is completely discharged;

the manipulator module 400 lifts the fifth dummy bottle head 702 upwards, and opens the liquid inlet at the upper end of the culture bottle; the manipulator module 400 pushes the sixth dummy bottle head 713 downwards, and closes the liquid outlet at the lower end of the culture bottle;

the manipulator module 400 then pulls the first needle 118 out of the bottle of the emollient rinse and places it back into the control infusion module 700. The manipulator module 400 clamps and moves the film moistening and cleaning liquid bottle 111 to the tray to complete the moistening and cleaning of the culture bottle.

Step two, transferring and filtering the high-capacity bottled water solution sample:

the manipulator module 400 grips and moves the large-capacity bottle 121 containing the water solution test sample in the tray, and places the large-capacity bottle 121 into the second turnover mechanism 602, and the following transfer process of the water solution of the large-capacity bottle is the same as the process of transferring the membrane moistening cleaning solution to the culture bottle, except that the membrane moistening solution bottle is replaced with a large-capacity bottle.

Step three, transferring the medium in the medium bottle 104 into a culture bottle:

the manipulator module 400 clamps and moves the container adapter converter 608 in the tray into the turnover mechanism 602, and then clamps and moves a bottle of culture medium bottle filled with culture medium in the tray into the turnover mechanism 602, and holds and positions the bottle;

then, the manipulator module 400 clamps and moves the first needle 118 in the tool module 700, inserts the first needle 118 into the bottle filled with culture medium, and the manipulator module 400 presses the second horizontal clamp 719 and the third horizontal clamp 720 to block the second pressure pipe infusion tube 723 and the third pressure pipe infusion tube 724 and prevent the culture medium in the bottle filled with culture medium from flowing in; the turnover mechanism module 600 turns over the inverted culture medium bottle and starts the peristaltic pump 502 to pump the culture medium into the first culture bottle 731, and closes the peristaltic pump 502 when the culture medium reaches the set injection amount; the turnover mechanism module 600 turns over the upright culture medium bottle, and the manipulator module 400 pulls out the first needle in the culture medium bottle and puts the first needle back to the original position; the manipulator module 400 grips and moves the medium bottles to the tray.

Then, the manipulator module 400 clamps and moves another culture medium bottle filled with culture medium in the tray, and places the culture medium bottle into the turnover mechanism 602, and holds and positions the culture medium bottle; the manipulator module 400 grasps and moves the first needle 118, inserting the needle into the medium bottle; the manipulator module 400 presses the first horizontal clamp 719, lifts the second horizontal clamp 719, opens the second pressure pipe infusion tube 723, and repeats the same medium pumping process as the first culture bottle 731; a bottle of culture medium bottle filled with culture medium is placed in the turnover mechanism 602 and is tightly held and positioned in the manipulator module 400 for clamping and moving the tray; the manipulator module 400 grasps and moves the first needle 118, inserting the needle into the medium bottle; the manipulator module 400 presses the second horizontal clamp 719, lifts the third horizontal clamp 720, opens the third pressure tube infusion tube 724, repeats the same medium pumping process, and presses the third horizontal clamp 720 to be in a closed state when the third culture bottle reaches a medium with a set injection amount; the manipulator module 400 grips, moves the container adapter transition 608 and puts back to the tray;

at the moment, the three horizontal clamps are all in a closed state of tightly pressing the three pressure pipe infusion tubes;

step four, bacterium collection is completed:

the manipulator module 400 grips and moves the first hose mount assembly 106 to the control infusion module 700; the manipulator module 400 pushes the fifth dummy bottle head 702 downwards, and closes the liquid inlet at the upper end of the culture bottle; the manipulator module 400 presses the clamping pieces of the first tube clamping clamp 741, the second tube clamping clamp 742 and the third tube clamping clamp 743 in the control infusion module 700 into the fixed slots to clamp infusion tubes connected with the culture bottle, and then the manipulator module 400 presses the first movable blade 751, the second movable blade 752 and the third movable blade to cut off the three infusion tubes. The control infusion module 700 placed on the infusion operation platform 344 is unlocked, and the manipulator module 400 grips and moves the control infusion module 700 back to the tray.

The guide rails transport the first tray module 100 out of the bacteria-collecting operation area, and the next tray module enters the bacteria-collecting operation area for next bacteria collection.

EXAMPLE 2: 10ml ampoules were filled with the aqueous solution test article.

As shown in fig. 15 and 16, the intelligent bacteria-collecting system in this embodiment includes a second tray module 130, an open bottle module 300, a manipulator module 400, a peristaltic pump module 500, a turnover mechanism module 600, and a control infusion module 700.

The bottle opening module 300 and the peristaltic pump module 500 are oppositely arranged, a guide rail is arranged between the bottle opening module 300 and the peristaltic pump module 500, and the second tray module 130 is placed on the guide rail; the bottle opening module 300 is fixed by a bottle opening module fixing plate; the manipulator module 400 and the peristaltic pump module 500 are fixed on the infusion operation platform 344. The control infusion module 700 is placed on the tray module 100, can be moved to the infusion operation platform 344 by the manipulator module 400, and fixes the infusion operation platform 344 by the cylinder and the positioning pin.

This embodiment is different from embodiment 1 in that: the tray modules differ in composition and the present embodiment further includes a decapping module 300.

As shown in fig. 17, the second tray module 130 comprises a tray, ampoule assemblies (ampoule 312, ampoule fixing plate 313), a chute 310, a control infusion module 700, a membrane cleaning solution bottle 111, a transition bottle 119, a triple needle fixture 319, a tray support rod 120, a second needle 103, a second hose mounting assembly 102 and a culture medium bottle 104.

In this embodiment, the transition bottle 119 is an empty bottle and only plays a role of transitioning the ampoule bottle to the bacteria collecting bottle.

The ampoule bottle assembly and the blanking groove 310 are arranged on the side close to the bottle opening module 300, so that the bottle opening module 300 can open bottles and blank bottle heads conveniently. Set up tray bracing piece 120 in tray four corners and be used for the stack of a plurality of trays, convenient transportation.

The second hose mounting assembly 102 has the same structure as the first hose mounting assembly 106, and includes a hose clamp and a single/multiple infusion tube fixed to the hose clamp, one end of each infusion tube is connected to the second needle 103 through a pipeline, and the other end of the infusion tube is connected to a liquid outlet of the triple needle fixture 319, so as to suck a sample into the transition bottle.

As shown in fig. 18, the triple needle fixture 319 includes a first needle jaw 324, a second needle jaw 325, three needles 323, and a seventh dummy bottle head 322. The first needle gripper 324 and the second needle gripper 325 grip the three needles 323 to constitute a liquid suction device. The seventh dummy vial 322 is disposed on the first needle gripper 324 for gripping by the manipulator module 400.

As shown in fig. 19 and 22, the decapping module 300 of the present embodiment has two types.

As shown in fig. 19-21, the bottle opening module includes a linear module 301 (in this example, a linear sliding table of a ball screw with silver KK86-740a 1), a bottle opening and lifting cylinder 210, a liquid pumping and lifting cylinder 203, a bottle head nesting plate 211, a breaking plate 213, a top cover plate 212, a top cover cylinder 208, a bottle opening and breaking cylinder 206, a needle mounting plate 318, and a knock pin cylinder 204.

The bottle opening lifting cylinder 210 and the liquid pumping lifting cylinder 203 are connected with the linear module 301 and are driven by the linear module 301 to move horizontally; the bottle opening lifting cylinder 210 is connected with the bottle head nesting plate 211 and drives the bottle head nesting plate 211 to move vertically; a top cover plate 212 is arranged above the bottle head embedded plate 211, and the top cover plate 212 is connected with the top cover cylinder 208 and moves horizontally under the driving of the top cover cylinder 208; the top cover plate 212 and the top cover cylinder 208 are both arranged on the bottle opening lifting cylinder 210 and move together with the bottle head nesting plate 211; the bottle head embedded plate 211 is connected with the bottle opening breaking cylinder 206 through a breaking plate 213 and is driven by the bottle opening breaking cylinder 206 to move horizontally; the liquid pumping lifting cylinder 203 is connected with the needle mounting plate 318 and drives the needle mounting plate 318 to move vertically. The needle mounting plate 318 is provided with a knock pin cylinder 204 and a clamping groove, and the knock pin cylinder and the clamping groove are matched with a mounting hole of the triple needle fixture 319 to fix the triple needle fixture 319. The bottle head nesting plate 211 is provided with a bottle head nesting hole, and a rubber ring is arranged in the bottle head nesting hole.

When the three-needle-head clamping device works, the manipulator module 400 clamps the three-needle-head tool 319 to the needle head mounting plate 318, and the knock pin cylinder 204 is matched with the clamping groove to fix the three-needle-head tool 319; the linear module 301 drives the whole bottle opening mechanism to move, so that the bottle head embedding plate 211 reaches the position above the first row of ampoule bottles, the bottle opening lifting cylinder 210 descends, and the bottle head embedding plate 211 is embedded in the first row of ampoule bottles; the bottle opening and breaking cylinder 206 moves to drive the bottle head embedding plate 211 to move to break the ampoule bottle, and the bottle head is clamped under the action of a rubber ring inside the bottle head embedding plate 211; the linear module 301 moves to enable the triple needle to be located above the opened ampoule bottle, the liquid pumping lifting cylinder 203 descends, and the needle on the triple needle tooling 319 enters the ampoule bottle to be extracted to obtain a test article. The straight line module 301 moves and sends the bottle head nested plate 211 with the ampoule bottle heads to the top of the blanking groove 310, the top cover cylinder 208 moves to drive the top cover plate 212 to move downwards, so that the bottle heads are pressed into the blanking groove 310, and then the straight line module 301 drives the whole bottle opening mechanism to repeat bottle opening and liquid pumping processes to the top of the next row of ampoule bottles.

As shown in fig. 22 to 24, another bottle opening module includes a machine body, a linear module 301, a bottle opening device, a material pouring device and a liquid pumping device, wherein the linear module 301, the bottle opening device, the material pouring device and the liquid pumping device are all disposed on the machine body. The linear module 301 is arranged at the top of the machine body and is connected with the bottle opening device, the material pouring device and the liquid pumping device through a module adapter plate 302; the module adapter plate 302 is connected with a connecting plate on the linear module 301 through a bolt; a bottle opening device and a material pouring device are arranged on one side of the module adapter plate 302, and a liquid pumping device is arranged on the other side of the module adapter plate 302; during operation, the discharging side of the bottle opening device is opposite to the feeding side of the pouring device.

In the embodiment, the linear module 301 adopts a linear sliding table with a silver feeding KK86-740A1 ball screw to drive the bottle opening device, the material pouring device and the liquid pumping device to horizontally move along the track;

the bottle opening device comprises a first push rod motor 303, an inclined plane press-breaking plate 306 and a spring push rod 321, wherein the first push rod motor 303 is fixed on one side of the module adapter plate 302, and an output shaft of the first push rod motor 303 is fixedly provided with the inclined plane press-breaking plate 306; the inclined plane press-breaking plate 306 is driven by the first push rod motor 303 to move vertically to press-break the head of the ampoule bottle 312.

The middle and two sides of the inclined plane breaking plate 306 are provided with plate surfaces to prevent broken ampoule bottle heads from toppling left and right after being broken, a spring push rod 321 is arranged below the inclined plane breaking plate, a spring 320 is arranged in the spring push rod, and the spring push rod 321 pushes the ampoule bottle heads into the front material pouring lower plate 307 at the moment when the ampoule bottle heads are broken.

As shown in fig. 24, the material pouring device includes an ampoule bottle adapter plate 311, a material pouring lower plate 307, a rear baffle 308, a short connecting rod 326, a long connecting rod 309, a slider 304, a slider connecting rod 317 (see fig. 25) and a rotating motor 305, the ampoule bottle adapter plate 311 is disposed at the bottom of the module adapter plate 302, the ampoule bottle adapter plate 311 in this embodiment is connected with an edge plate surface of the module adapter plate 302 through a bolt, and the ampoule bottle adapter plate 311 is tangent to an upper end surface of an ampoule bottle fixing plate 313 and can be driven by the linear module 301 to move horizontally, so as to achieve the purpose of respectively breaking each row of ampoule bottles. As shown in fig. 25, the front end surface of the lower pouring plate 307 has an inclined top angle 327 for supporting the neck of the ampoule bottle, so as to press the head of the ampoule bottle in cooperation with the inclined press-breaking plate.

The material pouring lower plate 307 is fixedly arranged on the ampoule bottle adapter plate 311, the rear baffle plate 308 is arranged on the discharging side of the material pouring lower plate 307 through a short connecting rod 326 and a long connecting rod 309 which are arranged on two sides of the material pouring lower plate 307, and the rear baffle plate 308 can rotate along the mounting point; one end of a long connecting rod 309 is fixed with the ampoule bottle adapter plate 311, the other end of the long connecting rod 309 is provided with a sliding block 304, and the sliding block 304 is connected with an output shaft of the rotating motor 305 through a sliding block connecting rod 317; the rotary electric machine 305 is fixed to a lateral extension plate 328 connected to the module adapter plate 302.

As shown in fig. 26, the fluid pumping device comprises a second push rod motor 314, an intermediate connecting plate 315, a suction needle adaptor 316 and a needle mounting plate 318, which are connected in sequence from top to bottom in this embodiment: the second push rod motor 314 is arranged at the other side of the module adapter plate 302; an intermediate connecting plate 315 is fixedly arranged on an output shaft of the second push rod motor 314, the intermediate connecting plate 315 is connected with a suction needle adaptor 316, and the needle mounting plate 318 is connected with the suction needle adaptor 316.

During operation, the manipulator module grasps the bottle-shaped head 322 of the triple needle head tooling 319, conveys the bottle-shaped head to the upper side of the needle head mounting plate 318 and then moves the bottle-shaped head to the clamping groove of the needle head mounting plate 318, and meanwhile, the triple needle head tooling 319 is clamped tightly by the fastening screws on two sides of the triple needle head tooling 319. The sharp module 301 drives whole bottle opening mechanism and removes for slope pressure break board 306 reachs the first row of ampoule top, and the push rod of first push rod motor 303 descends, drives slope pressure break board 306 and descends, and the front end apex angle top of falling material hypoplastron 307 terminal surface is at the weakest position of first row of ampoule bottleneck, through the interact with slope pressure break board 306, thereby presses disconnected first row and treats fungus collection ampoule bottle head. After the ampoule bottle head is pushed into the lower pouring plate 307 by the spring push rod 321, the linear module 301 moves to above the chute 310. The rotating motor 305 rotates to drive the sliding block adapter plate 317 to rotate, a sliding block is connected to the sliding block adapter plate 317, the sliding block 304 is connected with the long connecting rod 309, and the long connecting rod 309 can swing along the installation point due to the action of the sliding block 304. The long connecting rod 309 is respectively connected with the ampoule bottle adapter plate 311 and the rear baffle 308, and the connection points of the long connecting rod and the ampoule bottle adapter plate are respectively connected through cylindrical pins. A turnover mechanism is formed among the long connecting rod 309, the dumping lower plate 307, the rear baffle 308 and the ampoule bottle fixing plate 311, a fixed hinge point is formed by the cylindrical pin top and the ampoule bottle fixing plate 311, and the long connecting rod 309 is driven by the rotating motor 305 to rotate left and right around the fixed hinge point, so that the long connecting rod 309 serves as a driving rod of the tipping mechanism to rotate. The long connecting rod is connected with the rear baffle through a cylindrical pin top, the rotation of the long connecting rod 309 drives the rear baffle 308 to move forwards, the rear baffle 308 is connected with the material pouring lower plate 307 through a cylindrical pin, and the material pouring lower plate 307 is connected with the ampoule bottle fixing plate 311, so that the rear baffle 308 drives the material pouring lower plate 307 to turn around the cylindrical pin connected with the ampoule bottle fixing plate 311, and the ampoule bottle head is poured into the blanking groove 310. The linear module 301 drives the whole bottle opening mechanism to move, so that the triple needle head is positioned above the opened ampoule bottle; the second push rod motor 314 pushes the push rod downwards, so as to drive the triple needle head tooling 319 to suck the test sample in the broken ampoule bottle. And repeating the operation process to complete the opening and liquid extraction of the next row of ampoule bottles in sequence.

The operation process of the embodiment is as follows:

initial state of the control infusion module 700: the upper cap plug blocks the hole at the upper end of the culture bottle, the lower cap plug 705 blocks the liquid outlet at the lower end of the culture bottle, and the three horizontal clamps are in an open state.

Step one, rinsing a culture flask: the procedure for rinsing the flask was the same as that of example 1.

Step two, extracting the sample in the ampoule bottle to a transition bottle:

the bottle opening module 300 opens all the ampoules filled with the aqueous solution test sample, and loses the waste heads to the discharging chute 310;

the manipulator module 400 clamps and moves the empty transition bottle 119 on the tray, places the empty transition bottle in the turnover mechanism 601, and holds the empty transition bottle tightly for positioning; the manipulator module 400 grips and moves the second hose mounting assembly 102 to place in the hose mounting assembly mounting slot in the first peristaltic pump 501; the triple needle head tooling 319 in the mechanical arm gripping and moving module fixes the triple needle head tooling with the needle head mounting plate 318. The robotic arm picks up, moves the second needle 103 in the tray and inserts the needle into the transition bottle 119. The second push rod motor 314 drives the push rod to move downwards to insert the second needle 103 into a group of ampoule bottles filled with the aqueous solution test sample, and simultaneously the peristaltic pump 501 is started to transfer the aqueous solution test sample in the row of ampoule bottles into the transition bottle 119; the second push rod motor 314 drives the push rod to move upwards to pull out the second needle 103, the movement of the linear module 301 drives the triple needle tool 319 to be above the next group of ampoule bottles filled with the aqueous solution test article, then the needle is inserted into the ampoule bottles, and the aqueous solution test article in the ampoule bottles is transferred into the transition bottles; repeating the operation until all the water solution test products in the ampoule bottles are transferred into the transition bottle, and closing the first peristaltic pump 501;

then, the manipulator module 400 sequentially clamps and moves the triple needle fixture 319, the second needle 103, and the transition bottle 119 to the original position on the tray.

Step three, transferring the sample in the transition bottle 119 to a culture bottle of the control infusion module 700: the same procedure as the transfer of the membrane-wetting rinse to the culture flask was followed, except that the membrane-wetting rinse bottle was replaced with a transition bottle.

Step four, transferring the medium in the medium bottle 104 into a culture bottle: this procedure was the same as the procedure for transferring the medium into the culture flask in example 1.

Step five, bacterium collection is completed: the procedure was the same as in example 1.

Example 3: a water-soluble solid test sample is bottled in a 10ml penicillin bottle.

As shown in fig. 27 and 28, the present embodiment includes an open bottle module 300 (only for a pump function, not related to an open bottle), a robot module 400, a peristaltic pump module 500, a flip module 600, a control infusion module 700, and a third tray module 160.

This embodiment is different from embodiment 2 in that: the third tray module 160 is different in the sample, and the transition bottle 119 is filled with a dissolving solution, which can dissolve the powder in the vial.

As shown in FIG. 29, the third tray module 160 includes a tray, a vial assembly 161 (vial, vial holder), a chute 310, a control infusion module 700, a bottle 111 of a membrane cleaning solution, a transition bottle 119, a triple needle fixture 319, a second needle 103, a second hose mounting assembly 102, a medium bottle 104, and a container adapter 608.

The operation process of the embodiment is as follows:

initial state of the control infusion module 700: the upper cap plug blocks the hole at the upper end of the culture bottle, the lower cap plug 705 blocks the liquid outlet at the lower end of the culture bottle, and the three horizontal clamps are in an open state.

Step one, rinsing a culture flask: the procedure for rinsing the flask was the same as that of example 1.

Step two, dissolving penicillin bottle powder:

the manipulator module 400 clamps and moves the transition bottle 119 with the dissolving liquid on the tray, and the transition bottle is placed in the turnover mechanism 601 and is tightly held and positioned; the manipulator module 400 grips and moves the second hose mounting assembly 102 to place in the hose mounting assembly mounting slot in the first peristaltic pump 501; the manipulator module 400 grips and moves the triple needle head fixture 319 in the tray, and fixes it with the needle head mounting plate 318; the manipulator module 400 grabs and moves the second needle 103 in the tray and inserts the needle into the transition bottle. The second push rod motor 314 drives the push rod to move downwards to insert the needle head of the triple needle head tooling 319 into a group of penicillin bottles filled with water-soluble solid test articles; the turnover mechanism module 600 turns over the inverted transition bottle 119, turns on the first peristaltic pump 501 to turn it back, transfers the solution to the penicillin bottle, and turns off the first peristaltic pump 501 when the solution reaches a set volume; the second push rod motor 314 drives the push rod to move downwards to pull the needle inserted into the penicillin bottle to the bottle mouth without pulling out, the turnover mechanism module 600 turns over the upright transition bottle 119, the first peristaltic pump 501 is started to rotate forwards, so that the solution remained in the hose returns to the transition bottle 119, and then the first peristaltic pump 501 is closed; the second push rod motor 314 drives the push rod to move downwards to pull out the needle head inserted into the penicillin bottle and move to the next group of penicillin bottles filled with water-soluble solid test samples; the step is repeated, so that all the penicillin bottles are filled with the dissolving solution.

Step three, transferring the dissolved penicillin bottle powder to a transition bottle 119: the procedure was the same as in example 2, in which the sample was drawn from the ampoule into the transition flask.

Step four, transferring the test solution in the transition bottle 119 to a culture bottle: the same procedure as the transfer of the membrane-wetting rinse to the culture flask was followed, except that the membrane-wetting rinse bottle was replaced with a transition bottle.

Step five, transferring the culture medium in the culture medium bottle 104 into a culture bottle: this procedure was the same as the procedure for transferring the medium into the culture flask in example 1/2.

Step six, completing bacterium collection: the procedure was the same as in example 1/2.