阅读说明：本技术 一种具有生物活性的盖髓剂及其制备方法 (Pulp capping agent with bioactivity and preparation method thereof ) 是由 宋佳 孙德刚 王玲香 李�杰 谈波 于 2019-11-12 设计创作，主要内容包括：本发明属于牙体修复技术领域,尤其是一种具有生物活性的盖髓剂及其制备方法,现提出如下解决方案,包括主料和辅料,主料包括氢氧化钙、硬脂酸锌、氧化锌、氨磺酰乙基甲苯、二氧化钛溶液、硫酸钙溶液、丁二醇酯溶液和碳酸钙溶液,所述辅料包括骨形成蛋白、纤维蛋白粘结剂和转化生长因子。通过在原料中添加骨形成蛋白、纤维蛋白粘结剂和转化生长因子作为生物活性物质添加至盖髓剂内,能够诱导牙髓成纤维细胞、内皮细胞增殖并抑制炎症反应,提高牙髓修复能力,促进牙本质桥形成和牙髓组织的修复工作,具有抗菌、灭活内毒素、中和细菌酸性产物、为组织矿化提供碱性环境、诱导硬组织形成的功效。(The invention belongs to the technical field of tooth restoration, and particularly relates to a marrow capping agent with biological activity and a preparation method thereof. The bone morphogenetic protein, the fibrin adhesive and the transforming growth factor are added into the raw materials as bioactive substances and are added into the pulp capping agent, so that the proliferation of dental pulp fibroblasts and endothelial cells can be induced, the inflammatory reaction can be inhibited, the dental pulp repair capacity can be improved, the formation of a dental root bridge and the repair work of dental pulp tissues can be promoted, and the effects of resisting bacteria, inactivating endotoxin, neutralizing bacteria acidic products, providing an alkaline environment for tissue mineralization and inducing the formation of hard tissues can be achieved.)

1. The marrow capping agent with biological activity comprises main materials and auxiliary materials, and is characterized in that the main materials comprise calcium hydroxide, zinc stearate, zinc oxide, sulfamoylethyl toluene, titanium dioxide solution, calcium sulfate solution, butanediol ester solution and calcium carbonate solution, the auxiliary materials comprise bone morphogenetic protein, fibrin adhesive and transforming growth factors, and the main materials and the auxiliary materials are prepared from the following components in parts by weight: 46-58 parts of calcium hydroxide, 0.2-0.6 part of zinc stearate, 12-14 parts of zinc oxide, 34-46 parts of sulfamoyl ethyl toluene, 42-48 parts of titanium dioxide solution, 0.3-0.8 part of calcium sulfate solution, 34-46 parts of butanediol ester solution, 12-20 parts of calcium carbonate solution, 4-6 parts of bone morphogenetic protein, 6-8 parts of fibrin adhesive and 3-7 parts of transforming growth factor.

2. The preparation method of the pulp capping agent with bioactivity is characterized by comprising the following preparation steps:

the method comprises the following steps: adding a butanediol ester solution and a titanium dioxide solution into a reaction kettle, slowly adding a calcium carbonate solution, calcium hydroxide, sulfamoyl ethyl toluene and zinc oxide into the reaction kettle, and fully stirring for 2-4h after the calcium carbonate solution, the calcium hydroxide, the sulfamoyl ethyl toluene and the zinc oxide are added;

step two: adding zinc stearate, bone morphogenetic protein, fibrin adhesive and transforming growth factor into a reaction kettle, continuously dropwise adding a calcium sulfate solution in the adding process, fully stirring for 1h after dropwise adding is finished, and then standing;

step three: and separating the solution in the reaction kettle by a centrifugal machine to extract supernatant, and filtering the supernatant to obtain the pulp capping agent.

3. The bioactive myeloablative agent of claim 1, wherein the bone morphogenetic protein is a protein extracted from vertebrates and arthropods, and the transforming growth factor is transforming growth factor- β extracted from mammalian bones.

4. The method for preparing a pulp capping agent with bioactivity according to claim 1, wherein the reaction kettle in the first step is stirred at 45r/min, and the reaction kettle in the second step is stirred at 25 r/min.

5. The method for preparing a bioactive pulp capping agent according to claim 1, wherein the pulp capping agent prepared in the step five is used in combination with clove oil at a ratio of 3:2-5: 2.

Technical Field

The invention relates to the technical field of tooth restoration, in particular to a pulp capping agent with bioactivity and a preparation method thereof.

Background

Caries has become a major disease endangering oral cavity and even human health currently, WHO has classified caries as the third disease endangering human health, with the incidence increasing year by year. The pain is severe, and the physical and mental health, mood and work of patients are seriously affected. Further progression of the disease can cause pulposis, periapical disease, and osteomyelitis of the jaw, severely affecting the general health of the patient. In addition, caries eventually causes tooth loss due to development, affects digestive function, and causes malnutrition and physical deterioration of the whole body.

At present, the superficial caries and the middle caries can be treated by silver mercury filling, glass ion cement filling, resin filling and the like, and the deep caries is required to preserve living marrow as far as possible under the condition of correctly judging the pulp condition. Once the pulp is removed, the dental tissue is weakened because good nutrition is not supplied, and the chewing function is not performed. The pulp capping operation is a treatment method for protecting living pulp, and a preparation with the effect of recovering pulp lesions is covered on the dentin surface close to the pulp or the exposed pulp wound surface to protect the pulp and eliminate the lesions.

Disclosure of Invention

The invention provides a pulp capping agent with bioactivity and a preparation method thereof, which solve the problems that the biocompatibility of calcium hydroxide is not ideal and the induced dental pulp generates incomplete dentinal bridges in the use process of the existing pulp capping agent.

In order to achieve the purpose, the invention adopts the following technical scheme:

the marrow capping agent with biological activity comprises main materials and auxiliary materials, wherein the main materials comprise calcium hydroxide, zinc stearate, zinc oxide, sulfamoyl ethyl toluene, a titanium dioxide solution, a calcium sulfate solution, a butanediol ester solution and a calcium carbonate solution, the auxiliary materials comprise bone morphogenetic protein, a fibrin adhesive and a transforming growth factor, and the main materials and the auxiliary materials are prepared from the following components in parts by weight: 46-58 parts of calcium hydroxide, 0.2-0.6 part of zinc stearate, 12-14 parts of zinc oxide, 34-46 parts of sulfamoyl ethyl toluene, 42-48 parts of titanium dioxide solution, 0.3-0.8 part of calcium sulfate solution, 34-46 parts of butanediol ester solution, 12-20 parts of calcium carbonate solution, 4-6 parts of bone morphogenetic protein, 6-8 parts of fibrin adhesive and 3-7 parts of transforming growth factor.

Preferably, the invention also provides a preparation method of the pulp capping agent with biological activity, which specifically comprises the following preparation steps:

the method comprises the following steps: adding a butanediol ester solution and a titanium dioxide solution into a reaction kettle, slowly adding a calcium carbonate solution, calcium hydroxide, sulfamoyl ethyl toluene and zinc oxide into the reaction kettle, and fully stirring for 2-4h after the calcium carbonate solution, the calcium hydroxide, the sulfamoyl ethyl toluene and the zinc oxide are added;

step two: adding zinc stearate, bone morphogenetic protein, fibrin adhesive and transforming growth factor into a reaction kettle, continuously dropwise adding a calcium sulfate solution in the adding process, fully stirring for 1h after dropwise adding is finished, and then standing;

step three: and separating the solution in the reaction kettle by a centrifugal machine to extract supernatant, and filtering the supernatant to obtain the pulp capping agent.

Preferably, the bone morphogenetic protein is a protein extracted from a vertebrate or an arthropod, and the transforming growth factor is transforming growth factor- β extracted from a bone of a mammal.

Preferably, the reaction kettle in the first step is stirred at 45r/min, and the reaction kettle in the second step is stirred at 25 r/min.

Preferably, the pulp capping agent prepared in the fifth step is matched with clove oil for use, and the matching ratio is set to be 3:2-5: 2.

Compared with the prior art, the invention has the beneficial effects that:

according to the invention, the bone morphogenetic protein, the fibrin adhesive and the transforming growth factor are added into the pulp capping agent as bioactive substances, so that the dental pulp fibroblast and endothelial cell proliferation can be induced, the inflammatory reaction can be inhibited, the dental pulp repair capability can be improved, the dental pulp bridge formation and the dental pulp tissue repair work can be promoted, the dental pulp repairing agent has the effects of resisting bacteria, inactivating endotoxin, neutralizing bacteria acidic products, providing an alkaline environment for tissue mineralization and inducing hard tissue formation, can form a dental pulp bridge on the exposed dental pulp surface, has high physical strength, improves the dental pulp repair capability and has obvious effect.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.