阅读说明：本技术 一种用于治疗黄褐斑的透明质酸钠凝胶及其制备方法 (Sodium hyaluronate gel for treating chloasma and preparation method thereof ) 是由 田晨颖 李超 庞萌萌 韩月梅 刘建建 郭学平 于 2019-12-05 设计创作，主要内容包括：本发明公开了一种用于治疗黄褐斑的透明质酸钠凝胶及其制备方法,所述用于治疗黄褐斑的透明质酸钠凝胶含有以下物质：透明质酸钠、川芎提取物、当归提取物、桔梗提取物、聚乙烯吡咯烷酮、甘油、尼铂金乙酯、纯化水。其中,川芎提取物、当归提取物、桔梗提取物为祛斑美白功效的活性成分,三者结合共达化瘀祛斑功效；透明质酸钠、甘油为皮肤保湿剂,二者的添加对于活性成分的发挥具有协同增效作用；聚乙烯吡咯烷酮为高分子表面活性剂,使凝胶澄明并提高制剂的稳定性；尼铂金乙酯为防腐剂。本发明成分简单,制得的凝胶剂成品均匀稳定,有较强的祛斑活性。(The invention discloses a sodium hyaluronate gel for treating chloasma and a preparation method thereof, wherein the sodium hyaluronate gel for treating chloasma comprises the following substances: sodium hyaluronate, ligusticum wallichii extract, angelica sinensis extract, platycodon grandiflorum extract, polyvinylpyrrolidone, glycerol, ethylplatinum and purified water. Wherein, the ligusticum wallichii extract, the angelica sinensis extract and the platycodon grandiflorum extract are active ingredients with the effects of removing freckles and whitening, and the combination of the three components achieves the effects of removing blood stasis and removing freckles; the sodium hyaluronate and the glycerin are skin moisturizers, and the addition of the sodium hyaluronate and the glycerin has a synergistic effect on the exertion of active ingredients; polyvinylpyrrolidone is a high molecular surfactant, so that the gel is clear and the stability of the preparation is improved; the ethylparaben is a preservative. The invention has simple components, and the prepared gel finished product is uniform and stable and has stronger freckle removing activity.)

1. A sodium hyaluronate gel for treating chloasma, which comprises sodium hyaluronate, a ligusticum wallichii extract, an angelica sinensis extract, a platycodon grandiflorum extract, polyvinylpyrrolidone, glycerol, ethylplatinum and purified water.

2. The sodium hyaluronate gel according to claim 1, wherein the sodium hyaluronate has a molecular weight of 100 to 300 ten thousand daltons, preferably 150 to 250 ten thousand daltons.

3. The sodium hyaluronate gel according to claim 1 or 2, wherein the sodium hyaluronate is 0.5 to 1.5 parts by weight, preferably 0.8 to 1.2 parts by weight, based on 100 parts by weight of the total gel.

4. The sodium hyaluronate gel according to any one of claims 1 to 3, characterized in that, with respect to 100 parts by weight of total gel,

the weight portion of the ligusticum wallichii extract is 0.7-14,

0.3-6 parts by weight of the angelica sinensis extract,

the platycodon extract is 0.5-10 parts by weight.

5. The sodium hyaluronate gel according to any one of claims 1 to 4, wherein the polyvinylpyrrolidone is present in an amount of 0.5 to 1 part by weight, preferably 0.6 to 0.9 part by weight, based on 100 parts by weight of the total gel.

6. The sodium hyaluronate gel according to any one of claims 1 to 5, wherein the pH value of the sodium hyaluronate gel is in the range of 6.0 to 7.0.

7. A method for preparing the sodium hyaluronate gel of any one of claims 1 to 6, comprising the steps of:

weighing polyvinylpyrrolidone, adding the polyvinylpyrrolidone into purified water, and stirring for dissolving to obtain a solution A; and

then weighing a ligusticum wallichii extract, an angelica sinensis extract and a platycodon grandiflorum extract, adding the ligusticum wallichii extract, the angelica sinensis extract and the platycodon grandiflorum extract into the solution A, and uniformly stirring to obtain a solution B; and

then weighing sodium hyaluronate, adding the sodium hyaluronate into the solution B, and uniformly stirring to obtain a solution C; and

weighing ethylparaben, adding the ethylplatinum into glycerol, and uniformly stirring to obtain a solution D; and

and slowly adding the solution D into the solution C, stirring while adding until the solution is uniformly mixed, and thus obtaining the sodium hyaluronate gel for treating chloasma.

8. The method for preparing sodium hyaluronate gel according to claim 8, wherein polyvinylpyrrolidone is added in an amount of 0.5 to 1 part by weight in step 1.

9. The method for preparing sodium hyaluronate gel according to claim 8, wherein the amount of the ligusticum wallichii extract added in step 2 is 0.7-14 parts by weight, the amount of the angelica sinensis extract added is 0.3-6 parts by weight, and the amount of the platycodon grandiflorum extract added is 0.5-10 parts by weight.

10. The method for preparing sodium hyaluronate gel according to claim 9, wherein the amount of sodium hyaluronate added in step 3 is 0.5 to 1.5 parts by weight.

Technical Field

The invention relates to the technical field of hyaluronic acid, in particular to a sodium hyaluronate gel containing traditional Chinese medicines and used for treating chloasma, and a preparation method of the sodium hyaluronate gel used for treating chloasma.

Background

Chloasma is a chronic skin disease caused by the accumulation of pigments on female faces and is represented by symmetrical brown spots and plaques on the faces. In recent years, the number of patients suffering from chloasma is increasing year by year due to the influence of social mental pressure, atmospheric environmental pollution and other factors, and epidemiological studies at home and abroad show that the incidence rate of women is higher than that of men, and most of the patients occur at the birth age, and the incidence rate is as high as 50%. On the other hand, the chloasma disease is repeated for a long time and does not decline for years, and the psychological diseases of the patient can be caused because the diseased part is the face, which has great influence on the life quality of the patient.

With the improvement of living standard and shape beauty of people, the treatment of chloasma is also becoming an important subject of common research in dermatology and cosmetology. At present, clinically, decolorants, opacifiers and the like are used for treating chloasma, but although the decolorants, the opacifiers and the like can produce certain short-term effects, the long-term use of the decolorants, the opacifiers and the opacifiers can stimulate the skin of a patient, the skin of the patient can produce adverse reactions such as allergy, even carcinogenic risks exist, and the recurrence rate is high. In comparison, the traditional Chinese medicine combines the traditional decoction preparation for oral administration and the traditional Chinese medicine preparation for external use in the aspect of treating chloasma, has the advantages of natural and wide raw materials, small toxic and side effects, good treatment effect, obvious resource advantages and wide development and application prospects.

Most of traditional Chinese medicine preparations are lotion, ointment, cream and the like, wherein the lotion has high liquidity, is inconvenient to use and carry, and is easy to volatilize to cause unnecessary loss of medicines; the disadvantages of not very well spreadable pastes and creams, often resulting in uneven application and contamination of the clothing during use, lead to reduced patient compliance with the dosage forms, which also greatly limits the clinical use of the dosage forms. Compared with the preparation formulation, the gel has uniform texture, can be uniformly coated on the surface of the skin, does not have the defect of high fluidity due to certain viscosity, is convenient to carry and use, does not pollute clothes, has the characteristics of high drug release speed, long retention time, no stimulation to the skin, high bioavailability and avoidance of first pass effect of the liver, and is popular with patients in recent years.

CN104645027B discloses a paste for removing chloasma and a preparation method thereof, wherein the paste for removing chloasma is a paste prepared by mixing 18-23 parts of radix angelicae, 15-20 parts of cassia twig, 22-27 parts of motherwort herb, 12-17 parts of wolfberry fruit, 5-10 parts of spina gleditsiae, 18-23 parts of gypsum, 12-17 parts of melastoma dodecandrum root, 16-21 parts of bovea, 10-15 parts of garden balsam, 23-28 parts of mint, 35-40 parts of fructus broussonetiae, 28-33 parts of American ginseng, 31-36 parts of radix melastoma dodecandrum, 27-32 parts of Chinese date, 29-34 parts of lithospermum, 12-17 parts of burdock and the like according to parts by weight. The paste for removing chloasma can be used for treating chloasma by regulating liver, regulating qi-flowing, resolving stagnation, invigorating spleen, promoting blood circulation, removing blood stasis, regulating and invigorating qi and blood, balancing yin and yang, and promoting qi and blood circulation. However, the paste is not very spread, which often causes uneven coating and also causes contamination of clothes during use.

CN106309627A discloses a chloasma-removing gel, which is composed of the following raw and auxiliary materials in parts by weight: 30-40 parts of cynoglossum zeylanicum root, 10-20 parts of feverfew leaves, 20-30 parts of angelica, 20-40 parts of ligusticum wallichii, 10-20 parts of peach kernel, 1-3 parts of 940 carbomer, 10-30 parts of propylene glycol, 10-20 parts of azone, 5-10 parts of sodium hyaluronate and a proper amount of triethanolamine. The preparation method comprises the following steps: mixing cynoglossum zeylanicum root, Chinese lizardtail leaf, angelica, ligusticum wallichii and peach kernel, crushing, treating in a traditional Chinese medicine pretreatment machine, adding water into the treated medicinal materials, decocting twice, wherein the water amount is 10 times of the weight of the medicinal materials, decocting for 2 hours, mixing the decoctions, concentrating until the relative density is 1.10 at 65 ℃, adding ethanol until the volume percentage of the alcohol content reaches 65%, stirring, standing, filtering, concentrating the filtrate under reduced pressure until the relative density is 1.20 at 65 ℃, recovering the ethanol, drying the concentrated solution in an extract dryer, adding 940 carbomer, propylene glycol, azone, sodium hyaluronate and triethanolamine, and melting at about 75 ℃ to obtain the cynoglossum zeylanicum extract. However, the gel has the defects that the carbomer matrix can form the gel only by adding alkaline components such as triethanolamine and the like, and is easily influenced by other components such as saline electrolyte and the like, so that the viscosity of the gel is reduced.

CN102302650A discloses a traditional Chinese medicine composition for beauty treatment and spot removal, which comprises the following raw materials of effective components in parts by weight: 10-15 parts of astragalus membranaceus, 10-15 parts of angelica sinensis, 6-10 parts of ligusticum wallichii, 6-10 parts of radix paeoniae alba, 10-15 parts of radix rehmanniae preparata, 6-10 parts of radix bupleuri, 6-10 parts of safflower carthamus, 6-10 parts of platycodon grandiflorum, 10-15 parts of Chinese yam, 10-15 parts of lucid ganoderma, 10-15 parts of polygonum multiflorum and 10-15 parts of medlar. The preparation method comprises weighing the above components, respectively cleaning and pulverizing, performing microwave extraction to extract effective components, concentrating and drying the effective components to obtain dry powder; then the dry powder is mixed evenly and is prepared into the required preparation according to the conventional preparation method after sterilization treatment. But has the defects of large prescription amount, different effective components of a plurality of traditional Chinese medicines and low utilization degree and slow effect of the effective components by adopting the same extraction mode.

CN107638370A discloses a whitening and freckle-removing skin care product, which comprises, by mass, 0.1-5% of a papaya stem cell extract, 0.5-6% of a lemon extract, 0.5-8% of an angelica sinensis extract, 0.8-6% of a platycodon grandiflorum extract, 5-10% of esters, 5-8% of oils, 0.1-0.4% of a thickening agent, 15-20% of a humectant, 2-8% of an emulsifier, 0.1-1.0% of a preservative, 0.1-0.4% of triethanolamine, 0.1-1.0% of an essence, and the balance deionized water; the thickening agent is one or a combination of more of carbomer 980, xanthan gum and acrylate, and the humectant is one or a combination of more of micromolecular sodium hyaluronate, trehalose, glucan and tremella polysaccharide. The lemon extract, the angelica extract and the platycodon root extract in the composition have synergistic effect with the pawpaw stem cell extract, so as to play a synergistic effect in whitening and removing freckles. But has the disadvantages that a certain amount of neutralizing agent needs to be added in the gelling process, and the lubricating effect is poor.

CN109745387A discloses a gel coating agent for treating acne, which comprises the following components in percentage by mass: 6.0 to 10.0 percent of film forming agent, 0.5 to 2.0 percent of excipient, 3.0 to 8.0 percent of filling agent, 6.0 to 10.0 percent of humectant/plasticizer, 1.0 percent of essence, 10 percent of ethanol, 5.0 to 10.0 percent of medicine and purified water which are added to the total amount of 100 percent. The medicine comprises 1 part of rhubarb, 1 part of phellodendron, 1 part of scutellaria, 1.2 parts of cortex mori, 1 part of angelica and 0.1 part of sulfur, wherein the film-forming agent is polyvinyl alcohol (PVA), the excipient is sodium carboxymethyl cellulose (CMC-Na), the filler is polyvinylpyrrolidone (PVP), the humectant/plasticizer is glycerol, and the essence is osmanthus essence. The traditional Chinese medicine compound for treating acne has definite curative effect, treats both symptoms and root causes, and has obvious long-term effect. However, the disadvantage is that the gel prepared from polyvinyl alcohol is prone to "slushing" after coating.

Disclosure of Invention

In order to solve the problems, the invention provides a sodium hyaluronate gel containing traditional Chinese medicines for treating chloasma and a preparation method thereof on the basis of the modern traditional Chinese medicine theory and in combination with the transdermal absorption theory.

In order to achieve the purpose, the invention adopts the following technical scheme.

1. A sodium hyaluronate gel for treating chloasma, which comprises sodium hyaluronate, a ligusticum wallichii extract, an angelica sinensis extract, a platycodon grandiflorum extract, polyvinylpyrrolidone, glycerol, ethylplatinum and purified water.

2. The sodium hyaluronate gel according to item 1, wherein the molecular weight of the sodium hyaluronate is 100 to 300 ten thousand daltons, preferably 150 to 250 ten thousand daltons.

3. The sodium hyaluronate gel according to item 1 or 2, wherein the sodium hyaluronate is 0.5 to 1.5 parts by weight, preferably 0.8 to 1.2 parts by weight, based on 100 parts by weight of the total gel.

4. The sodium hyaluronate gel according to any one of claims 1 to 3, wherein the gel is prepared by mixing the sodium hyaluronate and the water soluble polymer in an amount of 100 parts by weight based on the total weight of the gel,

the weight portion of the ligusticum wallichii extract is 0.7-14,

0.3-6 parts by weight of the angelica sinensis extract,

the platycodon extract is 0.5-10 parts by weight.

5. The sodium hyaluronate gel according to any one of claims 1 to 4, wherein the polyvinylpyrrolidone is 0.5 to 1 part by weight, preferably 0.6 to 0.9 part by weight, based on 100 parts by weight of the total gel.

6. The sodium hyaluronate gel according to any one of claims 1 to 5, wherein the pH value of the sodium hyaluronate gel is in the range of 6.0 to 7.0.

7. A method for preparing a sodium hyaluronate gel according to any one of claims 1 to 6, comprising the steps of:

step 1: weighing polyvinylpyrrolidone, adding the polyvinylpyrrolidone into purified water, and stirring for dissolving to obtain a solution A; and

step 2: then weighing a ligusticum wallichii extract, an angelica sinensis extract and a platycodon grandiflorum extract, adding the ligusticum wallichii extract, the angelica sinensis extract and the platycodon grandiflorum extract into the solution A, and uniformly stirring to obtain a solution B; and

and step 3: then weighing sodium hyaluronate, adding the sodium hyaluronate into the solution B, and uniformly stirring to obtain a solution C; and

and 4, step 4: weighing ethylparaben, adding the ethylplatinum into glycerol, and uniformly stirring to obtain a solution D; and

and 5: and slowly adding the solution D into the solution C, stirring while adding until the solution is uniformly mixed, and thus obtaining the sodium hyaluronate gel for treating chloasma.

8. The method for preparing a sodium hyaluronate gel according to item 8, wherein the polyvinylpyrrolidone is added in an amount of 0.5 to 1 part by weight in step 1.

9. The method for preparing sodium hyaluronate gel according to item 8, wherein the amount of the ligusticum wallichii extract added in step 2 is 0.7-14 parts by weight, the amount of the angelica sinensis extract added is 0.3-6 parts by weight, and the amount of the platycodon grandiflorum extract added is 0.5-10 parts by weight.

10. The method for preparing a sodium hyaluronate gel according to item 9, wherein the amount of sodium hyaluronate added in step 3 is 0.5 to 1.5 parts by weight.

11. The method for preparing a sodium hyaluronate gel according to item 10, wherein the amount of ethylplatinum added in step 4 is 0.1 to 0.5 parts by weight.

12. The method for preparing sodium hyaluronate gel according to any one of claims 7 to 11, wherein the method for preparing ligusticum wallichii extract comprises the following steps: weighing a proper amount of ligusticum wallichii medicinal materials, shearing into pieces, adding an ethanol solution, carrying out reflux extraction, and then filtering to obtain medicine residues and a primary filtering medicine liquid; adding the residue into ethanol solution, reflux extracting, filtering, collecting medicinal liquid, and mixing with the first-time filtered medicinal liquid; centrifuging the combined liquid medicine, and concentrating the centrifuged supernatant at low temperature under reduced pressure; and freeze-drying the obtained concentrated solution to obtain the ligusticum wallichii extract.

13. The method for preparing the sodium hyaluronate gel according to any one of claims 7 to 12, wherein the method for preparing the angelica sinensis extract comprises the following steps: weighing a proper amount of angelica sinensis medicinal materials, crushing, sieving by a 80-mesh sieve, adding an ethanol solution, performing reflux extraction, and filtering to obtain medicine residues and a primary filtering medicine liquid; adding the residue into ethanol solution, reflux extracting, filtering, collecting medicinal liquid, and mixing with the first-time filtered medicinal liquid; concentrating the combined liquid medicine under reduced pressure at low temperature; and carrying out freeze-drying treatment on the obtained concentrated solution to obtain the angelica sinensis extract.

14. The method for preparing a sodium hyaluronate gel according to any one of claims 7 to 13, wherein the method for preparing the platycodon grandiflorum extract comprises the following steps: weighing a proper amount of platycodon grandiflorum medicinal materials, crushing, sieving with a 80-mesh sieve, adding an ethanol solution, performing reflux extraction, and filtering to obtain medicine residues and a primary filtering medicine liquid; adding the residue into ethanol solution, reflux extracting, filtering, collecting medicinal liquid, and mixing with the first-time filtered medicinal liquid; concentrating the combined liquid medicine under reduced pressure at low temperature; and carrying out freeze-drying treatment on the obtained concentrated solution to obtain the platycodon grandiflorum extract.

15. The method for preparing a sodium hyaluronate gel according to any one of claims 7 to 14, wherein the molecular weight of the sodium hyaluronate used in step 3 is 100 to 300 ten thousand daltons, preferably 150 to 250 ten thousand daltons.

16. The method for preparing a sodium hyaluronate gel according to any one of claims 7 to 15, wherein the pH value of the obtained sodium hyaluronate gel is in the range of 6.0 to 7.0.

Effects of the invention

The sodium hyaluronate gel provided by the invention can overcome the defects of large dosage, low bioavailability, slow effect, inconvenience in medication, strong sticky feeling, poor skin feeling and the like of the existing medicine for treating chloasma, and has the advantages of simple components, high safety, uniformity and stability of the prepared gel finished product, good comfort and strong freckle removing effect.

Detailed description of the invention

The sodium hyaluronate gel for treating chloasma provided by the invention comprises sodium hyaluronate, ligusticum wallichii extract, angelica sinensis extract, platycodon grandiflorum extract, polyvinylpyrrolidone, glycerol, ethylplatinum and purified water. Sodium Hyaluronate (HA) as a substance essential to human physiology is widely present in intercellular substance in living body, HAs no species difference, immunogenicity, safety, no toxicity, good biodegradability and biocompatibility. Its water solubility has viscosity, water retention and lubricity. HA HAs a good moisturizing effect and is known as an ideal natural moisturizing factor. The skin care product added with HA is compatible with skin and can quickly permeate into the deep layer of the skin; a hydration film is formed on the surface of the skin, and the moisture of the stratum corneum is effectively kept. The HA is used in the whitening and freckle-removing skin care product and HAs a synergistic effect. The large ion function of HA can regulate the flow and change of positive ions on the surface of a cell membrane and around cells, change the diffusion rate of substances in the skin, participate in the delivery of hydrated electrolytes, maintain the normal permeability of an arterial wall, improve the blood circulation of capillary vessels after degradation, have potential advantages in the aspects of transmission and transportation in the skin, and can help to prevent and treat skin diseases.

In a specific embodiment of the present invention, the molecular weight of the sodium hyaluronate is 100 to 300 ten thousand daltons, and more preferably 150 to 250 ten thousand daltons. For example, the sodium hyaluronate of the present invention may have a molecular weight of 150 ten thousand, 200 ten thousand, 250 ten thousand daltons, or the like.

In one embodiment of the present invention, the amount of the sodium hyaluronate is 0.5 to 1.5 parts by weight, for example, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4 parts by weight, and more preferably 0.8 to 1.2 parts by weight, based on 100 parts by weight of the total amount of the sodium hyaluronate gel.

Chuan Xiong has the functions of promoting blood circulation and activating qi, dissipating blood stasis and relieving pain, dispelling wind and eliminating dampness, and belongs to the qi-in-blood medicine. Beginning with Shen nong Ben Cao Jing, Chuan Xiong has been recorded in ancient times for whitening skin because of the traditional Chinese medicine theory that Chuan Xiong has whitening effect. Wei Yongqi considers that the mechanism of chloasma is the obstruction of the channels due to the abundance of damp turbidity and toxin stasis in the body, and the treatment principle is to disperse and resolve dampness, remove blood stasis and dredge collaterals. The organic phenolic acid is a main characteristic component in the ligusticum wallichii, and pharmacological research shows that the ferulic acid can obviously improve the blood fluidity and achieve the effects of removing blood stasis and removing speckles.

Dang Gui is one of the most commonly used Chinese herbs, and often combined with Chuan Xiong. Dang Gui is good at nourishing blood and regulating blood, while Chuan Xiong is good at promoting blood circulation and dissipating blood, and the two herbs are used together to enhance the actions of activating blood and resolving stasis, nourishing blood and regulating blood. In addition, the two herbs are suitable for moistening dryness, so the moistening of Dang Gui can be used to treat pungent-dryness syndrome of Chuan Xiong, pungent-dryness syndrome of Chuan Xiong and preventing greasy syndrome of Dang Gui, remove blood stasis without consuming qi and blood, nourish blood without blood stagnation and qi stagnation. The record of the "Yi Zong jin Jian" (medical Zong golden seal): the angelica and the rhizoma ligustici wallichii are used as main medicines of blood system, are warm in nature and sweet and pungent in flavor, can harmonize blood with warm energy, can enrich blood with sweet energy, and can disperse blood with pungent energy, and the angelica is used for ancient people to tonify blood, or one time or more times of the angelica is used for ancient people, and the rhizoma ligustici wallichii is used for pungent movement, so that the angelica and the rhizoma ligustici wallichii are quick to ascend and disperse, and can hurt qi after passing. Therefore, the kou is famous and cannot be taken alone or for a long time. However, it is applied to qi depression and blood coagulation without any effect, so it is used to treat blood diseases with the assistance of Dang Gui, so it can remove blood stasis and regenerate blood.

The platycodon grandiflorum is a traditional Chinese medicinal material with homology of medicine and food in China, is bitter and pungent in taste, neutral in nature, enters lung channels, and has the effects of eliminating phlegm and stopping cough, ventilating lung qi, eliminating phlegm and expelling pus. Modern researches show that saponin components in platycodon grandiflorum have strong tyrosinase activity inhibiting effect, can effectively control the generation of melanin, meanwhile, platycodon grandiflorum saponin also has strong antioxidant activity, can slow the synthesis of the melanin through the removing effect on redundant oxygen free radicals, has good treatment effect on facial spots, and can be widely applied as an effective component for treating the spots.

In a specific embodiment of the present invention, the ligusticum wallichii extract is 0.7 to 14 parts by weight, for example, 0.7, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 parts by weight, the angelicae sinensis extract is 0.3 to 6 parts by weight, for example, 0.3, 1, 2, 3, 4, 5, 6 parts by weight, and the platycodon grandiflorum extract is 0.5 to 10 parts by weight, for example, 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 parts by weight, based on 100 parts by weight of the total gel. The ligusticum wallichii, the angelica sinensis and the platycodon grandiflorum are considered to have whitening effects in the traditional Chinese medicine, and the effects of promoting blood circulation, promoting qi circulation, removing blood stasis and removing speckles can be achieved by combining the ligusticum wallichii, the angelica sinensis and the platycodon grandiflorum.

In a specific embodiment of the present invention, the polyvinylpyrrolidone is 0.5 to 1 part by weight, for example, 0.5, 0.6, 0.7, 0.8, 0.9, 1 part by weight, and more preferably 0.6 to 0.9 part by weight, based on 100 parts by weight of the gel. The polyvinylpyrrolidone is a high molecular surfactant and can play a role in assisting dissolution and dispersing stability.

In an embodiment of the invention, the gel further comprises glycerol and ethylplatinum; the glycerin is contained in an amount of 0.2 to 1.2 parts by weight, for example, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2 parts by weight, and the ethylplatine is contained in an amount of 0.1 to 0.5 parts by weight, for example, 0.1, 0.2, 0.3, 0.4, 0.5 parts by weight, based on 100 parts by weight of the total gel. The glycerin is an excellent humectant, has no irritation to the skin, and can play a good moistening effect. The ethylplatinum is a good bacteriostatic preservative.

The pH value range of the sodium hyaluronate gel provided by the invention is 6.0-7.0. The pH value of the surface of normal skin is about 5.0-7.0, the skin can be in the best state of absorbing nutrition only within the normal pH value range, namely weak acidity, and the capability of resisting external erosion, elasticity, luster, moisture and the like of the skin are in the best state. The pH value of the sodium hyaluronate gel provided by the invention is 6.0-7.0, and the sodium hyaluronate gel is weak acidic and is most suitable for skin.

The preparation method of the sodium hyaluronate gel for treating chloasma, provided by the invention, comprises the following steps:

step 1: weighing polyvinylpyrrolidone, adding the polyvinylpyrrolidone into purified water, and stirring for dissolving to obtain a solution A; and

step 2: then weighing a ligusticum wallichii extract, an angelica sinensis extract and a platycodon grandiflorum extract, adding the ligusticum wallichii extract, the angelica sinensis extract and the platycodon grandiflorum extract into the solution A, and uniformly stirring to obtain a solution B; and

and step 3: then weighing sodium hyaluronate, adding the sodium hyaluronate into the solution B, and uniformly stirring to obtain a solution C; and

and 4, step 4: then, weighing the ethylparaben, adding the ethylplatinum into glycerol, and uniformly stirring to obtain a solution D; and

and 5: and slowly adding the solution D into the solution C, stirring while adding until the solution is uniformly mixed, and thus obtaining the sodium hyaluronate gel for treating chloasma.

In the step 1, the polyvinylpyrrolidone is added in an amount of 0.5 to 1 part by weight.

In step 2, the addition amount of the ligusticum wallichii extract is 0.7-14 parts by weight, the addition amount of the angelica sinensis extract is 0.3-6 parts by weight, and the addition amount of the platycodon grandiflorum extract is 0.5-10 parts by weight.

In the step 3, the addition amount of the sodium hyaluronate is 0.5-1.5 parts by weight.

In the step 4, the amount of ethylparaben added is 0.1-0.5 parts by weight.

In an embodiment of the present invention, the ligusticum wallichii extract in step 2 is prepared by the following steps: weighing a proper amount of ligusticum wallichii medicinal materials, shearing into pieces, adding an ethanol solution, carrying out reflux extraction, and then filtering to obtain medicine residues and a primary filtering medicine liquid; adding the residue into ethanol solution, reflux extracting, filtering, collecting medicinal liquid, and mixing with the first-time filtered medicinal liquid; centrifuging the combined liquid medicine, and concentrating the centrifuged supernatant at low temperature under reduced pressure; and freeze-drying the obtained concentrated solution to obtain the ligusticum wallichii extract.

In one embodiment of the present invention, the ligusticum wallichii extract in step 2 is prepared by the following steps: weighing a proper amount of ligusticum wallichii medicinal material, shearing into pieces, adding 70% ethanol solution which is 10 times of the weight of the medicinal material, carrying out reflux extraction for 3 hours, and then filtering to obtain medicine residues and primary filtering medicine liquid; adding the residue into 8 times of 70% ethanol solution, extracting under reflux for 3 hr, filtering, collecting medicinal liquid, and mixing with the first-time filtered medicinal liquid; treating the combined liquid medicine in a centrifuge at the speed of 4500r/min for 15 minutes, and carrying out low-temperature reduced pressure concentration on supernatant after centrifugal treatment at the temperature of 60 ℃ and the pressure of-0.06 to-0.09 MPa; and freeze-drying the obtained concentrated solution to obtain the ligusticum wallichii extract.

In an embodiment of the present invention, the preparation method of the angelica sinensis extract in step 2 comprises the steps of: weighing a proper amount of angelica sinensis medicinal materials, crushing, sieving by a 80-mesh sieve, adding an ethanol solution, performing reflux extraction, and filtering to obtain medicine residues and a primary filtering medicine liquid; adding the residue into ethanol solution, reflux extracting, filtering, collecting medicinal liquid, and mixing with the first-time filtered medicinal liquid; concentrating the combined liquid medicine under reduced pressure at low temperature; and carrying out freeze-drying treatment on the obtained concentrated solution to obtain the angelica sinensis extract.

In one embodiment of the present invention, the angelica sinensis extract in step 2 is prepared by the steps of: weighing a proper amount of Chinese angelica medicinal materials, crushing, sieving by a 80-mesh sieve, adding an ethanol solution with the concentration of 60 percent, the weight of which is 12 times that of the medicinal materials, carrying out reflux extraction for 1 hour, and then filtering to obtain medicine residues and a primary filtering liquid medicine; adding the residue into 60% ethanol solution 10 times the weight of the residue, reflux extracting for 1 hr, filtering, collecting medicinal liquid, and mixing with the first-time filtered medicinal liquid; carrying out low-temperature reduced pressure concentration on the combined liquid medicine at the temperature of 60 ℃ and the pressure of-0.06 to-0.09 MPa; and carrying out freeze-drying treatment on the obtained concentrated solution to obtain the angelica sinensis extract.

In an embodiment of the present invention, the method for preparing the platycodon grandiflorum extract in the step 2 comprises the steps of: weighing a proper amount of platycodon grandiflorum medicinal materials, crushing, sieving with a 80-mesh sieve, adding an ethanol solution, performing reflux extraction, and filtering to obtain medicine residues and a primary filtering medicine liquid; adding the residue into ethanol solution, reflux extracting, filtering, collecting medicinal liquid, and mixing with the first-time filtered medicinal liquid; concentrating the combined liquid medicine under reduced pressure at low temperature; and carrying out freeze-drying treatment on the obtained concentrated solution to obtain the platycodon grandiflorum extract.

In one embodiment of the present invention, the platycodon grandiflorum extract in the step 2 is prepared by the following steps: weighing a proper amount of platycodon grandiflorum medicinal material, crushing, sieving with a 80-mesh sieve, adding an ethanol solution with the concentration of 70% which is 10 times of the weight of the medicinal material, performing reflux extraction for 3 hours, and filtering to obtain medicine residues and a primary-filtered medicine liquid; adding the residue into 70% ethanol solution 8 times the weight of the residue, reflux extracting for 2 hr, filtering, collecting medicinal liquid, and mixing with the first-time filtered medicinal liquid; carrying out low-temperature reduced pressure concentration on the combined liquid medicine at the temperature of 60 ℃ and the pressure of-0.06 to-0.09 MPa; and carrying out freeze-drying treatment on the obtained concentrated solution to obtain the platycodon grandiflorum extract.

The pH value of the sodium hyaluronate gel obtained according to the specific embodiment of the invention ranges from 6.0 to 7.0.

Examples

The experimental methods used in the following examples are all conventional methods, unless otherwise specified.

Sodium hyaluronate used in the following examples was purchased from Huaxi Biotech Co., Ltd, polyvinylpyrrolidone was purchased from Miou chemical reagents Co., Tianjin City, and other materials, reagents, etc., were commercially available without specific description.