阅读说明：本技术 一种硫酸新霉素发酵培养基及发酵方法 (Neomycin sulfate fermentation medium and fermentation method ) 是由 刘铭 晏涛 何连明 于 2019-11-26 设计创作，主要内容包括：一种硫酸新霉素发酵培养基,所述培养基包括以下重量份配比的组分：菜籽饼粉150～250、米粉4000～8000、酵母粉50～100、氯化钠40～70、碳酸钙30～60、碳酸铵40～80、磷酸氢二钠2～100、磷酸二氢钾10～50、硅油0～50、a-淀粉酶10～20。本发明提供的一种硫酸新霉素发酵培养基及发酵方法,可以有效地降低发酵原材料成本,采用菜籽饼粉替代黄豆粕粉,提高发酵罐的装料系数,促进产量的提升,取得市场上的成本竞争优势。(A neomycin sulfate fermentation culture medium comprises the following components in parts by weight: 150-250 parts of rapeseed cake powder, 4000-8000 parts of rice flour, 50-100 parts of yeast powder, 40-70 parts of sodium chloride, 30-60 parts of calcium carbonate, 40-80 parts of ammonium carbonate, 2-100 parts of disodium hydrogen phosphate, 10-50 parts of monopotassium phosphate, 0-50 parts of silicone oil and 10-20 parts of alpha-amylase. The neomycin sulfate fermentation culture medium and the fermentation method provided by the invention can effectively reduce the cost of fermentation raw materials, and adopt rapeseed cake powder to replace soybean meal powder, thereby improving the charging coefficient of a fermentation tank, promoting the increase of yield and obtaining the cost competitive advantage in the market.)

1. A neomycin sulfate fermentation culture medium is characterized by comprising the following components in parts by weight:

150-250 parts of rapeseed cake powder, 4000-8000 parts of yeast powder and 50-100 parts of

40-70 parts of sodium chloride, 30-60 parts of calcium carbonate and 40-80 parts of ammonium carbonate

10-50 silicone oils of 2-100% potassium dihydrogen phosphate disodium salt

10-20 parts of alpha-amylase.

2. The neomycin sulfate fermentation medium of claim 1, which is characterized by comprising the following components in parts by weight:

rapeseed cake powder 200 rice flour 6000 yeast powder 75

Sodium chloride 60 calcium carbonate 50 ammonium carbonate 60

Disodium hydrogen phosphate 45 monopotassium phosphate 35 silicone oil 20

a-amylase 15.

3. A neomycin sulfate fermentation method is characterized by comprising the following steps:

step 1: sealing, drying and culturing the sandy soil spores to obtain a bevel spore suspension;

step 2: putting the bevel spore suspension prepared in the step 1 into a seeding tank in which a fermentation culture medium is placed in advance, and culturing for 36-48 h to obtain a culture solution;

and step 3: feeding the culture solution prepared in the step 2) into a fermentation tank with a preset fermentation culture medium for inoculation and fermentation for 140-160 h to obtain a fermentation solution, and continuously supplementing the fermentation culture medium in the fermentation process;

and 4, step 4: terminating the culture of the fermentation liquor, putting the fermentation liquor into an adsorption tank, adding resin into the fermentation liquor, diluting the fermentation liquor to a concentration of less than 13000u/ml, and neutralizing the pH value to 6.4-7.0 to obtain dilute fermentation liquor, thereby completing the fermentation step.

4. The neomycin sulfate fermentation process of claim 3, wherein: the incubation time in step 2 was 40 hours.

Technical Field

The invention relates to the technical field of neomycin sulfate fermentation, in particular to a neomycin sulfate fermentation culture medium and a fermentation method, wherein cheap organic nitrogen source rapeseed cake powder is used for replacing soybean meal.

Background

The neomycin producing strain needs to provide nutrients such as carbon source, nitrogen source, inorganic salt, trace elements and the like required by the growth of the neomycin producing strain in the fermentation, growth and metabolism processes. The carbon source mainly comprises organic carbohydrate substances of grains such as starch, rice flour and the like, the nitrogen source mainly comprises organic substances such as soybean meal powder, yeast powder, peanut cake powder and the like, the inorganic salt mainly comprises ammonium sulfate, sodium chloride, phosphate and the like, all the nutrient substances are combined together according to different proportions and called as a fermentation culture medium formula, the fermentation culture medium formula is further divided into a basic material formula and a fermentation intermediate supplement formula, the fermentation basic material formula refers to nutrient substances which are added when a fermentation tank is arranged on the fermentation tank, the content of the nutrient components is comprehensive, and the basic material formula is the most key part in the fermentation production process and is the basis for determining the fermentation level.

At present, the price of raw materials of an organic nitrogen source in a fermentation base material formula used for producing neomycin is generally higher, so that alternative raw materials are required to be continuously searched for effectively reducing the cost of fermentation raw materials and enhancing the competitiveness of products in the market, the fermentation process is innovative, and the cost of the raw materials is reduced to the maximum extent.

Disclosure of Invention

The invention aims to solve the technical problem of providing a neomycin sulfate fermentation culture medium and a fermentation method, which can effectively reduce the cost of fermentation raw materials, adopt rapeseed cake powder to replace soybean meal powder, improve the charging coefficient of a fermentation tank, promote the improvement of yield and obtain the cost competitive advantage in the market.

In order to solve the technical problems, the technical scheme adopted by the invention is as follows: a neomycin sulfate fermentation culture medium comprises the following components in parts by weight:

preferably, the weight parts of each component are as follows:

a neomycin sulfate fermentation process, comprising the steps of:

step 1: sealing, drying and culturing the sandy soil spores to obtain a bevel spore suspension;

step 2: putting the bevel spore suspension prepared in the step 1 into a seeding tank in which a fermentation culture medium is placed in advance, and culturing for 36-48 h to obtain a culture solution;

and step 3: feeding the culture solution prepared in the step 2) into a fermentation tank with a preset fermentation culture medium for inoculation and fermentation for 140-160 h to obtain a fermentation solution, and continuously supplementing the fermentation culture medium in the fermentation process;

and 4, step 4: and (3) terminating the culture of the fermentation liquor, putting the fermentation liquor into an adsorption tank, adding resin into the fermentation liquor, diluting the fermentation liquor to a concentration of less than 13000u/ml, and neutralizing the pH value to 6.4-7.0 to obtain dilute fermentation liquor, thereby completing the fermentation step.

Preferably, the incubation time in step 2 is 40 hours.

The neomycin sulfate fermentation medium and the fermentation method provided by the invention have the following beneficial effects:

1. effectively reduces the cost of fermentation raw materials and improves the competitive advantage of the product in the market.

2. The raw material source is wide: the rapeseed cake powder is derived from rapeseeds, and the rape is mainly planted in a wide area of the Yangtze river basin, so that the yield is huge; because the rapeseed cake powder which is a byproduct after oil extraction of rapeseed cakes has no wide application as compared with soybean meal powder, the rapeseed cake powder is supplied all the year round, and the product source can meet the production requirement.

3. The defoaming is facilitated, the charging coefficient of the fermentation tank is improved, the batch yield of the tank is improved, and the equipment utilization rate and the production efficiency are improved:

the foam is easily produced in the fermentation production process, the foam can reduce the dissolved oxygen in the fermentation liquor, the normal metabolism of hypha is influenced, the charging coefficient of a fermentation tank can be reduced, the tank placing volume is reduced, and therefore the tank batch yield is reduced. Therefore, good foam control is an important link for improving the fermentation level. Normally, fermentation often uses antifoams to eliminate foam. The antifoaming agent is expensive and too much amount causes side effects on fermentation. The rapeseed cake powder has a large oil content, can provide a certain amount of rapeseed oil while providing an organic nitrogen source, plays a role in inhibiting bubbles of fermentation liquor, and effectively increases the tank placing volume.

4. Considerable economic benefits are generated: if the new formula and the production process are implemented, preliminary statistics is carried out, the market price of the rapeseed cake powder is 1000 yuan/ton cheaper than that of the soybean meal powder, the raw material cost of an enterprise can be saved by about 20 ten thousand yuan per month, and the economic benefit of the enterprise can be increased by more than 200 ten thousand yuan per year.

Detailed Description