阅读说明：本技术 促进人体角质形成细胞增殖的原料组合物及其应用 (Raw material composition for promoting human keratinocyte proliferation and application thereof ) 是由 甘智斌 林娴婷 杨建中 于 2018-07-16 设计创作，主要内容包括：本发明提供一种促进人体角质形成细胞增殖的原料组合物,包括如下质量百分比的组分：麦芽低聚糖0.5～15％；葡聚糖0.001～2.5％；甘草提取物0.01～5％；蓝桉叶提取物1～40％；溶剂0～98.489％；余量的水。所述溶剂为醇类溶剂。本发明的原料组合物,具有保湿、抗炎、杀菌和止痒、促进人体角质形成细胞增殖等多种功能,可用于制备皮肤护理产品。在特定的配比前提下,麦芽低聚糖、葡聚糖等组分有效抑制了蓝桉叶提取物的絮凝,具有良好的稳定性,制备的皮肤护理产品同样具有良好的稳定性。用于头皮护理,可有效提高头皮的抗炎症能力,使头皮保持润湿、增强头皮的免疫力和抵抗力,本能调节皮肤细胞活性,促进头皮角质形成细胞增殖,进而防止脱发。(The invention provides a raw material composition for promoting human keratinocyte proliferation, which comprises the following components in percentage by mass: 0.5-15% of malto-oligosaccharide; 0.001-2.5% of glucan; 0.01-5% of licorice extract; 1-40% of a eucalyptus globulus leaf extract; 0-98.489% of a solvent; the balance of water. The solvent is an alcohol solvent. The raw material composition has multiple functions of moisturizing, resisting inflammation, sterilizing, relieving itching, promoting proliferation of human keratinocytes and the like, and can be used for preparing skin care products. Under the premise of a specific proportion, the components such as maltooligosaccharide and glucan effectively inhibit the flocculation of the eucalyptus globulus leaf extract, so that the stability is good, and the prepared skin care product also has good stability. The scalp care product is used for scalp care, can effectively improve the anti-inflammatory capability of the scalp, keep the scalp moist, enhance the immunity and the resistance of the scalp, intrinsically regulate the activity of skin cells, promote the proliferation of scalp keratinocytes and further prevent alopecia.)

1. The raw material composition for promoting the proliferation of human keratinocytes is characterized by comprising the following components in percentage by mass:

the solvent is an alcohol solvent.

2. The raw material composition for promoting the proliferation of human keratinocytes according to claim 1, comprising the following components in percentage by mass:

the solvent is an alcohol solvent.

3. The starting composition for promoting the proliferation of human keratinocytes according to claim 1 or 2, wherein said alcoholic solvent is selected from the group consisting of 1, 3-butanediol, ethanol, glycerol, and mixtures thereof.

4. The raw material composition for promoting proliferation of human keratinocytes according to claim 1 or 2, wherein the maltooligosaccharide comprises maltotriose and maltotetraose, and the mass percentage of the maltotetraose is 1 to 96%.

5. The raw material composition for promoting proliferation of human keratinocytes according to claim 1 or 2, wherein the glucan is a glucan isolated and purified from a culture solution of Saccharomyces nigricans.

6. The composition as claimed in claim 1 or 2, wherein the effective components of the licorice extract comprise dipotassium glycyrrhizinate and glabridin, wherein the dipotassium glycyrrhizinate accounts for 80-98% of the weight of the licorice extract, and the glabridin accounts for 0.001-0.2% of the weight of the licorice extract.

7. The raw material composition for promoting proliferation of human keratinocytes according to claim 1 or 2, wherein the solid content of the eucalyptus globulus leaf extract is 0.01 to 5 wt%.

8. The method for preparing a starting composition for promoting the proliferation of human keratinocytes according to any one of claims 1 to 7, comprising the following steps:

1) adding the liquorice extract, the eucalyptus globulus labill leaf extract and the solvent into a reaction container according to the formula ratio, and uniformly stirring to obtain a mixture A;

2) adding malto-oligosaccharide, glucan and water in the formula ratio into the mixture A prepared in the step 1), and uniformly stirring to obtain the raw material composition for promoting the proliferation of human keratinocytes.

9. Use of a starting composition for promoting proliferation of human keratinocytes as defined in any one of claims 1 to 8, for promoting proliferation of human keratinocytes.

10. Use of a starting composition for promoting the proliferation of human keratinocytes according to claim 9, characterized in that it is used for the preparation of scalp care products, preventing hair loss.

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to a raw material composition for promoting human keratinocyte proliferation and application thereof.

Background

Almost every consumer wants to have beautiful and healthy hair. The scalp is the soil in which hair grows. Just as the quality of the soil can directly affect crop harvest, the health of the scalp can directly affect the health of the hair, and thus the overall aesthetics of the individual.

Alopecia is one of the most common health problems of the scalp. Alopecia is caused by a number of causes, among which the effects of hormones on hair life cycle leading to alopecia are undisputed, but other factors can also affect and exacerbate alopecia. When the scalp is exposed to external environmental pressure and irritants such as pollutants, such as sunlight, air pollutants, smoke and other irritants, the scalp can trigger the immune function of the scalp to cause inflammation. The overstimulation of inflammatory reaction can affect blood circulation, on one hand, the nutrition needed by cell growth is insufficient, and the renewal activity of epidermal cells is delayed; on the other hand, the inflammation reaction products can not be excreted in time, so that the inflammation reaction products are accumulated continuously, the scalp becomes dry, the elasticity is reduced, and the scalp is aged. If the hair follicle becomes inflamed and the surrounding tissue hardens, resulting in poor hair anchorage and premature hair loss, the hair cannot grow properly. Therefore, scalp care is not always necessary for hair care, and attention to scalp care has been increasingly paid in recent years.

Healthy scalp care is primarily to eliminate adverse factors affecting scalp health, such as the removal of bacteria, fungi, excess unsaturated fatty acids, sebum oxide, and the like. Secondly, the scalp is protected from stimulation of excessive ultraviolet rays and pollutants such as PM 2.5, industrial waste gas and the like, and the defense functions such as barrier function, blood circulation, anti-inflammatory capability and the like of the scalp are improved by improving the environment. The core of scalp care is to maintain the normal metabolism of scalp cells, promote the proliferation of human keratinocytes and provide a guarantee for maintaining the normal metabolism of scalp cells.

Therefore, it is very important to develop a raw material composition for promoting the proliferation of human keratinocytes, which can be used for scalp care and thus prevent alopecia.

Disclosure of Invention

The inventor of the invention has intensively studied and found that the eucalyptus globulus labill leaf extract, the malto-oligosaccharide, the glucan and other components can form a stable raw material composition under a specific proportion, can effectively promote the proliferation of human keratinocytes, and can be used for preparing skin care products, such as scalp care products, body care products and cosmetics for preventing alopecia.

The first object of the present invention is to provide a raw material composition for promoting the proliferation of human keratinocytes, which can be used for preparing scalp care products, body care products and cosmetics.

In order to achieve the purpose, the invention adopts the following technical scheme:

the raw material composition for promoting the proliferation of human keratinocytes comprises the following components in percentage by mass:

the solvent is an alcohol solvent.

According to the invention, the eucalyptus globulus leaf extract, the malto-oligosaccharide, the glucan and other components can form a stable raw material composition in a specific proportion, and the proliferation of human keratinocytes can be effectively promoted. Wherein the malto-oligosaccharide can also act as a humectant and help to inhibit flocculation of the Eucalyptus globulus Labill leaf extract. When the mass percent of the malto-oligosaccharide is more than 0.5 percent, the raw material composition mixture is stable. The glucan also has moisturizing effect. Meanwhile, the glucan and the maltooligosaccharide act synergistically to inhibit the agglomeration of the eucalyptus globulus leaf extract. When the mass percentage of the glucan is less than 0.001%, the raw material composition mixture is unstable.

According to the present invention, when the content of the licorice extract exceeds 5% by mass, the stability of the raw material composition mixture is affected, and if the content is less than 0.01%, the effect expected by the present invention is weak.

According to the invention, the eucalyptus globulus leaf extract can cause skin irritation if the mass percentage of the eucalyptus globulus leaf extract exceeds 40%. When the content of the blue eucalyptus leaf extract is less than 1%, the effect expected by the present invention is weak.

The cell proliferation rate of human keratinocytes of the composition prepared under the specific formula conditions is 10-60%. Preferably 20 to 60%.

According to the present invention, preferably, the raw material composition for promoting proliferation of human keratinocytes comprises the following components by mass:

the solvent is an alcohol solvent.

Under the condition of the mass percentage, the raw material composition has better effect of promoting the proliferation of human keratinocytes.

According to the invention, the alcohol solvent is selected from one or more of 1, 3-butanediol, ethanol and glycerol.

Preferably, the alcoholic solvent is 1, 3-butanediol or ethanol or a mixture of the two.

Further preferably, the alcohol solvent is 1, 3-butanediol.

The water is preferably deionized water or purified water.

According to the invention, the malto-oligosaccharide is a polymer composed of alpha-glucose monomers, and comprises maltotriose and maltotetraose, wherein the mass percent of the maltotetraose is 1-96%.

Further, the compound also comprises maltose pentasaccharide.

According to the invention, the glucan is selected from glucan separated and purified from a saccharomyces cerevisiae culture solution.

According to the invention, the effective components of the licorice extract comprise dipotassium glycyrrhizinate and glabridin, wherein the mass percent of the dipotassium glycyrrhizinate in the licorice extract is 80-98%, and the mass percent of the glabridin in the licorice extract is 0.001-0.2%.

According to the invention, the extract of the leaves of the eucalyptus globulus is selected from the extract of the young leaves of the eucalyptus globulus in the growing period of 2-3 years. The young leaves of the blue eucalyptus in the growth period of 2-3 years are used as the raw material of the blue eucalyptus leaf extract, so that the high content of effective components in the blue eucalyptus leaf extract can be ensured.

In the invention, the eucalyptus globulus leaf extract can be in a liquid state without spray drying after extraction, and contains a solvent and a solid substance.

The Eucalyptus globulus Labill leaf extract can also be powder obtained by spray drying after extraction. In application, the powdered Eucalyptus globulus Labill leaf extract is diluted with solvent to form liquid.

The solvent is preferably a solvent used for extracting eucalyptus globulus leaves.

Preferably, the solid content of the eucalyptus globulus leaf extract is 0.01-5 wt%.

The second purpose of the invention is to provide a preparation method of the raw material composition, which comprises the following preparation steps:

1) adding the liquorice extract, the eucalyptus globulus labill leaf extract and the solvent into a container according to the formula ratio, and uniformly stirring to obtain a mixture A;

2) adding the malto-oligosaccharide, the glucan and the water in the formula ratio into the mixture A prepared in the step 1), and uniformly stirring to obtain the raw material composition.

In the step 1), the eucalyptus globulus labill leaf extract is preferably added with 1, 3-butanediol in a formula amount if the extract is extracted by 1, 3-butanediol. If ethanol is used for extraction, the amount of ethanol to be formulated is preferably added.

The third purpose of the invention is to provide the application of the raw material composition, which is used for promoting the proliferation of human keratinocytes.

According to the present invention, the above-described raw material composition can be used for preparing skin care products including scalp care products, body care products and cosmetics.

Further, according to the present invention, the above raw material composition can be used for preparing a scalp care product for promoting the proliferation of keratinocytes and preventing hair loss.

Compared with the prior art, the invention has the following beneficial technical effects:

(1) the raw material composition has multiple functions of moisturizing, resisting inflammation, sterilizing, relieving itching, promoting the proliferation of human keratinocytes and the like, and can be used for preparing skin care products, such as scalp care products, body care products and cosmetics.

(2) On the premise of a specific proportion, the components such as malto-oligosaccharide and glucan act together with the eucalyptus globulus labill leaf extract, and the glucan is used as an inclusion agent to uniformly disperse the eucalyptus globulus labill leaf extract and finally form an oil-in-water type water phase dispersion system, so that the flocculation of the eucalyptus globulus labill leaf extract is effectively inhibited, and the eucalyptus globulus labill leaf extract has good stability. The skin care products prepared with the composition of the present invention also have good stability, and thus, the use of the eucalyptus globulus leaf extract in the field of skin care products such as scalp care products, body care products and cosmetics is significantly improved.

(3) The scalp care agent is used for scalp care, can effectively improve the anti-inflammatory capacity of the scalp, keep the scalp moist, enhance the immunity and the resistance of the scalp, intrinsically regulate the activity of skin cells, promote the proliferation of scalp keratinocytes and further prevent alopecia.

Detailed Description

The present invention will be further described with reference to the following examples. It should be understood that the following examples are illustrative only and are not intended to limit the scope of the present invention.

In the following examples, the starting materials and equipment were all commercially available.

The malto-oligosaccharide is derived from hydrolyzed corn starch.

The glucan is selected from glucan separated and purified from a black yeast culture solution.

The Glycyrrhrizae radix extract is commercially available product and is purchased from pill-type pharmaceutical Co

The Eucalyptus globulus leaf extract is a commercially available product and is purchased from Nichio oil Co. Or extracting with alcohol solvent such as 1, 3-butanediol, ethanol and glycerol or mixture of alcohol solvents by conventional method.