阅读说明：本技术 一种紫茉莉提取物的应用及含其的皮肤外用剂 (Application of Mirabilis jalapa extracts and skin external preparation containing the same ) 是由 李慧 摩根多斯桑托斯 章漳 蒋丹丹 秦优 于 2018-07-20 设计创作，主要内容包括：本发明提供了一种紫茉莉提取物的应用及含其的皮肤外用剂。该应用具体为,紫茉莉提取物在制备抗氧化剂、黑色素合成抑制剂、弹性纤维水解抑制剂和抗皮肤衰老蛋白表达上调剂中的一种或多种的应用。紫茉莉提取物尤其是紫茉莉花提取物,具有清除自由基活性、抑制酪氨酸酶活性、抑制弹性蛋白酶活性和提高人成纤维细胞中抗衰老蛋白表达的作用,能够调控真皮中胶原蛋白的合成,具有延缓衰老功效。(The invention provides application of Mirabilis jalapa extracts and a skin external agent containing the same, wherein the application specifically comprises application of or more of Mirabilis jalapa extracts in preparing antioxidants, melanin synthesis inhibitors, elastic fiber hydrolysis inhibitors and anti-skin-aging protein expression up-regulating agents.)

Application of Mirabilis jalapa extracts in preparing or more of antioxidant, melanin synthesis inhibitor, elastic fiber hydrolysis inhibitor and skin aging protein expression up-regulating agent is provided.

2. The use of claim 1, wherein the antioxidant is a free radical scavenger;

and/or the melanin synthesis inhibitor is a tyrosinase inhibitor;

and/or the inhibitor of elastane hydrolysis is an elastase inhibitor;

and/or, the skin aging resistant protein expression up-regulator is or more of BMP-1 expression up-regulator, collagen I expression up-regulator and collagen V expression up-regulator.

3. The use according to claim 1 or 2,

when the mirabilis jalapa extract is used for preparing an antioxidant, the concentration of the mirabilis jalapa extract in the antioxidant is 0.025-0.075 mg/mL, and preferably 0.05 mg/mL;

when the mirabilis jalapa extract is used for preparing a melanin synthesis inhibitor, the concentration of the mirabilis jalapa extract in the melanin synthesis inhibitor is 0.25-0.75 mg/mL, preferably 0.5 mg/mL;

when the mirabilis jalapa extract is used for preparing the elastic fiber hydrolysis inhibitor, the concentration of the mirabilis jalapa extract in the elastic fiber hydrolysis inhibitor is 0.5-1.5 mg/mL, preferably 1 mg/mL;

when the mirabilis jalapa extract is used for preparing the skin aging resistant protein expression up-regulator, the concentration of the mirabilis jalapa extract in the skin aging resistant protein expression up-regulator is 0.05-1 mg/mL; preferably: when the anti-skin aging protein is BMP-1, the concentration of the Mirabilis jalapa extract in the up-regulator of the anti-skin aging protein expression is 0.05 mg/mL; when the anti-skin aging protein is collagen I or collagen V, the concentration of the mirabilis jalapa extract in the anti-skin aging protein expression up-regulator is 1 mg/mL.

4. The use of claim 1, wherein the Mirabilis jalapa extract is prepared by a method comprising the steps of: mixing Mirabilis jalapa L.with solvent, and extracting to obtain extractive solution.

5. The use of claim 4, wherein the Mirabilis jalapa extraction site is Mirabilis jalapa flower;

and/or the solvent is ethanol water solution, the concentration of the ethanol water solution is preferably 60-80%, and the percentage refers to volume percentage;

and/or the ratio of the mass of the mirabilis jalapa to the volume of the solvent is 1: (8-12) g/mL, preferably 1: 10 g/mL;

and/or the extraction method comprises the steps of cold soaking extraction and heating extraction in sequence; the time of the cold leaching extraction is preferably 12 hours; the temperature for heating and extracting is preferably 50-70 ℃, more preferably 60 ℃; the heating extraction time is preferably 1-3 h, more preferably 2 h; the number of times of the heating extraction is preferably 3;

and/or, the extracting solution is also subjected to purification treatment; preferably, the extract is chromatographed by macroporous adsorption resin; preferably, the extracting solution is dried, redissolved by water and then subjected to macroporous adsorption resin chromatography; the macroporous adsorption resin is preferably AB-8; the eluent for chromatography is preferably ethanol water solution, the concentration of the ethanol water solution is preferably 25-35%, more preferably 30%, and the percentage refers to volume percentage; the volume of eluent for the chromatography is preferably 5 column volumes; the chromatography is preferably further subjected to impurity removal treatment, more preferably to impurity removal by elution with water, and the elution volume of the water is preferably 1 column volume.

6, kinds of external preparations for skin, characterized in that, it contains 0.001-10% Mirabilis jalapa extract, the percentage is the weight percentage in the external preparations for skin;

preferably, the preparation method of the mirabilis jalapa extract comprises the following steps: mixing Mirabilis jalapa L.with solvent, and extracting to obtain extractive solution.

7. The external preparation for skin as claimed in claim 6, wherein the extract site of Mirabilis jalapa is Mirabilis jalapa flower;

and/or the solvent is ethanol water solution, the concentration of the ethanol water solution is preferably 60-80%, and the percentage refers to volume percentage;

and/or the ratio of the mass of the mirabilis jalapa to the volume of the solvent is 1: (8-12) g/mL, preferably 1: 10 g/mL;

and/or the extraction method comprises the steps of cold soaking extraction and heating extraction in sequence; the time of the cold leaching extraction is preferably 12 hours; the temperature for heating and extracting is preferably 50-70 ℃, more preferably 60 ℃; the heating extraction time is preferably 1-3 h, more preferably 2 h; the number of times of the heating extraction is preferably 3;

and/or, the extracting solution is also subjected to purification treatment; preferably, the extract is chromatographed by macroporous adsorption resin; preferably, the extracting solution is dried, redissolved by water and then subjected to macroporous adsorption resin chromatography; the macroporous adsorption resin is preferably AB-8; the eluent for chromatography is preferably ethanol water solution, the concentration of the ethanol water solution is preferably 25-35%, more preferably 30%, and the percentage refers to volume percentage; the volume of eluent for the chromatography is preferably 5 column volumes; the chromatography is preferably further subjected to impurity removal treatment, more preferably to impurity removal by elution with water, and the elution volume of the water is preferably 1 column volume.

8. The external preparation for skin as claimed in claim 6 or 7,

the content of the mirabilis jalapa extract is 0.0025-0.15%, preferably 0.005%, 0.05% or 0.1%, and the percentage refers to the weight percentage of the mirabilis jalapa extract in the skin external preparation;

when the mirabilis jalapa linnaeus extract is used as an antioxidant in the skin external preparation, the content of the mirabilis jalapa linnaeus extract is preferably 0.0025 to 0.0075 percent, more preferably 0.005 percent, and the percent refers to the weight percent in the skin external preparation;

when the mirabilis jalapa extract is used as a melanin synthesis inhibitor in the skin external preparation, the content of the mirabilis jalapa extract is preferably 0.025-0.075%, more preferably 0.05%, and the percentage refers to the weight percentage in the skin external preparation;

when the mirabilis jalapa extract is used as a skin elastic fiber hydrolysis inhibitor in the skin external preparation, the content of the mirabilis jalapa extract is preferably 0.05-0.15%, more preferably 0.1%, and the percentage refers to the weight percentage in the skin external preparation;

when the mirabilis jalapa extract is used as an up-regulator of the expression of anti-skin aging proteins in the skin external preparation, the content of the mirabilis jalapa extract is preferably 0.005-0.1%, more preferably 0.005% or 0.1%, and the percentage refers to the weight percentage in the skin external preparation; more preferably: when the anti-skin aging protein is BMP-1, the content of the Mirabilis jalapa extract is 0.005%, and the percentage refers to the weight percentage in the skin external preparation; when the skin aging resistant protein is collagen I or collagen V, the content of the Mirabilis jalapa extract is 0.1%, and the percentage refers to the weight percentage in the skin external preparation.

9. The external preparation for skin as claimed in claim 6 or 7, further comprising kinds of other active ingredients, preferably moisture-retaining active ingredient, antioxidant active ingredient, oil-controlling active ingredient, whitening active ingredient and anti-aging active ingredient;

and/or, the skin external agent further comprises a medium or a matrix excipient, wherein the excipient is preferably in the form of a water phase, an oil phase, a gel, a wax-in-water type emulsion, an oil-in-water type emulsion or a water-in-oil type emulsion;

and/or, the skin external agent further comprises or more of a preservative, a fragrance, a hydrophilic active agent and a lipophilic active agent;

and/or, the skin external agent further comprises a particulate phase, wherein the particulate phase is or more of pigments, pearling agents and fillers.

10. The external preparation for skin as claimed in claim 9,

when the external skin preparation is a toning lotion, the external skin preparation comprises, by weight, 5% of glycerin, 3-6% of a polyol A, 1% of betaine, 0.5% of panthenol, 0.05-0.1% of dipotassium glycyrrhizinate, 0.15-0.2% of polyethylene glycol-320.5%, 0.15-0.2% of a surfactant A, 0.4-0.45% of a preservative A, 0-0.15% of xanthan gum, 0.03% of a perfume and 0.001-10% of a mirabilis jalapa extract, and the balance being 100% by weight with deionized water, wherein the polyol A is or more of butylene glycol, 1, 2-pentanediol and dipropylene glycol, the surfactant A is polysorbate-20 and/or PEG-40 hydrogenated castor oil, the preservative A is methyl hydroxybenzoate and/or phenoxyethanol, the mirabilis jalapa preferably is a mirabilis jalapa jasmine extract, and the content of the mirabilis jalapa preferably is 0.0025-0.15%, more preferably 0.005-0.1%, and the percentage by weight of the external skin preparation is in percentage by weight;

when the external skin preparation is essence, the external skin preparation comprises, by weight, 5% of glycerin, 3-6% of a polyol B, 1% of panthenol, 0.1% of dipotassium glycyrrhizinate, 0.1% of allantoin, 0.05-3.6% of a surfactant B, 0.25-0.45% of a preservative B, 0.2-0.4% of xanthan gum, 0.20.3% of an acrylic resin Pemulen TR-20.3% of a fragrance, and 0.05-10% of a Mirabilis jalapa extract, and the balance being supplemented to 100% with deionized water, wherein the polyol B is butylene glycol and/or 1, 3-propylene glycol, the surfactant B is polyglycerol-2 oleate and/or polyglycerol-10 oleate, the preservative B is or more of propylparaben, methylparaben and phenoxyethanol, the Mirabilis jalapa extract preferably contains 0.0025-0.15%, more preferably 0.005-0.005% of the weight percentage of the external skin preparation;

when the skin external agent is emulsion, the skin external agent comprises the following components in percentage by weight: 5% of glycerol, 5% of 1, 3-propylene glycol, 1% of panthenol, 0.5% of tocopherol acetate, 2% of polydimethylsiloxane, 1652% of emulsifier A, 2-3% of oil C, 3% of isopropyl isostearate, 0.5% of cetostearyl alcohol, 0.3% of preservative C, 0.5% of xanthan gum and 0.1% of EDTA disodium; the skin external agent also comprises triethanolamine, and the content of the triethanolamine is based on adjusting the pH value of the skin external agent to 5.5-7; and 0.001% -10% of the mirabilis jalapa extract; the rest is supplemented to 100 percent by deionized water; wherein the oil C is mineral oil and/or isohexadecane; the preservative C is methyl hydroxybenzoate and/or propyl hydroxybenzoate; the mirabilis jalapa extract is preferably a mirabilis jalapa extract, and the content of the mirabilis jalapa extract is preferably 0.0025-0.15%, more preferably 0.005-0.1%, and the percentage refers to the weight percentage of the mirabilis jalapa extract in the skin external preparation;

when the external skin preparation is a cream, the external skin preparation comprises the following components in percentage by weight: 5% of glycerol, 5% of 1, 3-propylene glycol, 1% of panthenol, 0.5% of tocopherol acetate, 2% of polydimethylsiloxane, 1652% of emulsifier A, 2-8% of oil D, 3% of isopropyl isostearate, 2-3% of cetostearyl alcohol, 0.3% of preservative D, 2% of hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer and 0.1% of disodium EDTA; the skin external agent also comprises triethanolamine, and the content of the triethanolamine is based on adjusting the pH value of the skin external agent to 5.5-7; 0.001% -10% of mirabilis jalapa extract; the rest is supplemented to 100 percent by deionized water; wherein the oil D is mineral oil and/or isohexadecane; the preservative D is methyl hydroxybenzoate and/or propyl hydroxybenzoate; the mirabilis jalapa extract is preferably a mirabilis jalapa extract, and the content of the mirabilis jalapa extract is preferably 0.0025-0.15%, more preferably 0.005-0.1%, and the percentage refers to the weight percentage of the skin external preparation.

Technical Field

The invention relates to application of Mirabilis jalapa extracts and a skin external preparation containing the same.

Background

With the continuous development and the gradual maturity of the skin care product market, more and more brands emerge, and the requirement of consumers for the efficacy of the skin care product is expected to see the practical and effective skin care effect, so the research level of the efficacy mechanism of the active substances of the skin care product needs to be improved, the explanation of the efficacy mechanism becomes deeper and deeper, the simple description of the images cannot be realized, the advanced biotechnology needs to be fused, the discovery of the skin biology is deepened continuously with the continuous development of the cytobiology technical level, and more targets and regulation mechanisms related to the functional structure of the skin are discovered.

The skin is the largest organ of the human body, covers the whole body, and mainly plays a role in protecting the body, removing sweat, sensing cold and heat, pressure and the like, so that various tissues and organs in the body are prevented from being invaded by physical, mechanical, chemical and pathogenic microorganisms.

Skin aging is classified into endogenous aging and exogenous aging. Intrinsic aging is natural aging, an aging process caused by irresistible physiological factors with increasing age, belongs to gene-level aging, and is obviously characterized by the appearance of wrinkles and the laxity of the skin. Extrinsic aging is mainly aging caused by environmental factors, such as ultraviolet radiation, high temperature, cold, environmental pollution, nutritional deficiency, diseases, bad living habits, and the like, wherein ultraviolet radiation is the most important factor, and is therefore called photoaging, which is expressed by rough and dry skin exposed parts, wrinkle deepening and thickening, irregular pigmentation, vasodilatation, epidermal keratosis, dermal elasticity fibrosis, accumulation of degradation products, and the like, and can be theoretically prevented and slowed down. The theory of free radical aging states that excessive free radicals are considered to be an important cause of skin photoaging, and because the skin is exposed to external environments such as ultraviolet rays, active oxygen generated by the ultraviolet rays and active oxygen in other external environments generate various free radicals in the skin, so that the skin is damaged by oxidative stress, and finally, the skin is aged.

in general, dermal aging is manifested by a decrease in thickness, a decrease in synthesis and degradation of collagen and elastin, an increase in degradation of catabolic enzyme activity, a decrease in dermal thickness of 20-80% in the aging process, and a gradual degradation of tissues and functions, collagen is protein having the highest content in the human body, which imparts toughness, elasticity, water-proofing function, etc., and is an important raw material for maintaining the morphology and structure of the skin and tissue organs and repairing each damaged tissue, or is a main component of extracellular matrix.

BMP-1 (bone morphogenetic protein-1), which is the major protease in the assembly of extracellular matrix molecules, is involved in processing, primarily in the cleavage of peptide chains from proteins in the extracellular matrix (ECM), and can modify precursors of many biomolecules into biologically functional structural proteins and active enzymes, including many matrix molecules such as procollagen types I, III, V, and VII, Lipoxygenase (LOX), small leucine-rich proteoglycan (SLRP), etc. simply, BMP-1 functionalizes collagen, ensuring that collagen is modified in an ideal manner during biosynthesis, and that the correct protein conformation is formed.

Collagen is the gold standard in various indexes for resisting skin aging, but the efficacy and mechanism of most products on the cosmetic market are only about how to increase the collagen content (such as promoting generation, supplying and protecting). Recent studies have shown that the three-dimensional conformation and structure of collagen also have a significant effect on skin gloss. For the skin, collagen is a light propagation channel, and collagen fiber-mediated light scattering plays an important role in light propagation penetration inside the dermis. If the skin has high collagen content and normal and orderly arrangement structure, light can be directly projected into the skin, and the appearance of the skin has transparent luster; if the collagen is less, the structure is abnormal, and the arrangement is disordered, the diffuse reflection of the light path (the scattering effect of the collagen beam) is formed, so that the appearance is dark, yellow and matt.

Photoaging caused by UV exposure causes increased activity of skin elastase. Elastase is distributed in various tissues and cells, and the increase of elastase activity can cause the hydrolysis of skin elastic fibers, thereby accelerating the decomposition of collagen and elastin in dermis, leading the skin to lose elasticity, and causing skin aging phenomena such as wrinkles and the like.

The process of pigmentation is complex processes, mainly due to biochemical reactions within melanocytes, i.e. tyrosine, under the action of tyrosinase as a catalyst, produces dopaquinone, which in turn, under enzymatic or non-enzymatic oxidation, forms melanin, wherein the key is the synthesis of melanin, which is controlled by tyrosinase.

Therefore, the research on how to improve the anti-aging capability of the skin has important significance on delaying skin aging.

Extracts from natural plant sources are increasingly used in the field of skin care due to their long history of use, excellent multiple efficacy.

Mirabilis jalapa L is a plant of genus Mirabilis of family Mirabilis, also called as primula, Sophora subprostrata, Nostoc nocturna, Megalobus, and Eugenia caryophyllata. The native tropical America is cultivated in various places in China, namely south and north, and is an ornamental flower and sometimes a wild flower. The medicine is originally seen in Bencao gang mu Shi Yi (compendium of materia Medica), is cold in nature, sweet in taste, applicable to all kinds of medicines such as root, leaf, flower and seed, and can be used for treating symptoms such as heat stranguria, whitish and turbid urine, edema, leucorrhea with reddish discharge, joint swelling and pain, carbuncle, sore and pyogenic infections, and the seed powdery mildew can remove facial freckles and nevus. The research reports that the water extract of the mirabilis jalapa contains amino acids, saccharides, glycosides, organic acids, phenols, volatile oil and other substances, the alcohol extract contains phenols, cardiac glycosides, lactones, coumarins, flavones and other substances, and the petroleum ether extract mainly contains saponins and other substances.

Disclosure of Invention

The invention provides application of mirabilis jalapa extracts and a skin external preparation containing the same, aiming at solving the technical problem that active substances capable of improving the antioxidant and anti-aging capabilities of skin are lacked in the prior art.

The inventor discovers through a large amount of researches that: 1) mirabilis jalapa extract has free radical scavenging effect; 2) mirabilis jalapa extract has tyrosinase activity inhibiting effect; 3) the Mirabilis jalapa extract has effects of inhibiting elastase activity, improving anti-aging protein expression in human fibroblast, regulating synthesis of collagen in dermis, and delaying aging, and is suitable for cosmetics.

The invention mainly solves the technical problems by the following technical means:

the invention provides application of Mirabilis jalapa extracts in preparing or more of antioxidants, melanin synthesis inhibitors, elastic fiber hydrolysis inhibitors and anti-skin aging protein expression up-regulating agents.

In the present invention, the antioxidant may be a substance conventional in the art as , which helps to trap and neutralize free radicals, such as a free radical scavenger.

In the present invention, the melanin synthesis inhibitor may be a substance that inhibits melanin synthesis, such as a tyrosinase inhibitor, which is conventional in the art.

In the present invention, the elastic fiber hydrolysis inhibitor may be a substance that inhibits the hydrolysis of elastic fibers, which is conventional in the art, such as elastase inhibitor , wherein the hydrolysis of elastic fibers in the skin accelerates the decomposition of collagen and elastin in the dermis, thus causing the skin to lose elasticity and wrinkles.

In the present invention, the anti-skin aging protein may be a protein that is conventional in the art and is associated with skin aging, such as collagen and elastin.

In the present invention, the skin aging-resistant protein expression up-regulator may be any substance conventionally used in the art to affect the expression of skin aging-related proteins, such as or more of a BMP-1 (bone morphogenetic protein-1) expression up-regulator, a collagen I expression up-regulator and a collagen V expression up-regulator.

It is well known to those skilled in the art that BMP-1 can be processed to modify procollagen types I, III, V and VII to functionalize the collagen.

In the invention, the concentration of the mirabilis jalapa extract in the antioxidant is preferably 0.025-0.075 mg/mL, and more preferably 0.05 mg/mL.

In the present invention, the concentration of the mirabilis jalapa extract in the melanin synthesis inhibitor is preferably 0.25-0.75 mg/mL, and more preferably 0.5 mg/mL.

In the present invention, the concentration of the mirabilis jalapa extract in the elastic fiber hydrolysis inhibitor is preferably 0.5-1.5 mg/mL, and more preferably 1 mg/mL.

In the invention, the concentration of the mirabilis jalapa extract in the skin aging resistant protein expression up-regulator is preferably 0.05-1 mg/mL, such as 0.05mg/mL or 1 mg/mL.

When the anti-skin aging protein is BMP-1, the concentration of the Mirabilis jalapa extract in the up-regulator of the expression of the anti-skin aging protein is preferably 0.05 mg/mL.

When the anti-skin aging protein is collagen I or collagen V, the concentration of the mirabilis jalapa extract in the up-regulator of anti-skin aging protein expression is preferably 1 mg/mL.

In the present invention, the antioxidant, melanin synthesis inhibitor, elastic fiber hydrolysis inhibitor and anti-skin aging protein expression up-regulator are suitably used in cosmetics or skin care products.

In the invention, the Mirabilis jalapa extract can be prepared by a conventional method in the field, for example, the Mirabilis jalapa L is mixed with a solvent and extracted to obtain an extracting solution.

The extract part of Mirabilis jalapa can be parts of Mirabilis jalapa which can be used as medicine, such as kinds or more of Mirabilis jalapa root, Mirabilis leaf, Mirabilis jalapa flower and Mirabilis jalapa seed, preferably Mirabilis jalapa flower, and Mirabilis jalapa flower is obtained by harvesting Mirabilis jalapa L in bud stage.

When the extracting part of the mirabilis jalapa is mirabilis jalapa, the mirabilis jalapa extract is a mirabilis jalapa extract.

Wherein, the solvent can be an extraction solvent which is conventional in the field, and preferably ethanol. The concentration of the ethanol can be the conventional concentration in the field, and is preferably 60-80% ethanol water solution, and the percentage refers to volume percentage.

Wherein, the ratio of the quality of the mirabilis jalapa to the volume of the solvent can be a conventional ratio in the field, such as 1: (8-12) g/mL, preferably 1: 10 g/mL.

The extraction method can be conventional extraction method in the field, such as cold soaking extraction and heating extraction in sequence.

The time for the cold leaching extraction may be a time conventional in the art, for example 12 h.

The temperature for the heating extraction may be a temperature conventional in the art, for example, 50 to 70 ℃, preferably 60 ℃.

The time for the heating extraction can be a time conventional in the art, such as 1-3 h, preferably 2 h.

The number of times of the heating extraction may be a number of times conventional in the art, for example, 3 times. It is known to those skilled in the art that when the number of times of extraction is more than 1 by heating, the extracts should be combined.

Wherein the extract can be purified by conventional procedures in the art, for example, subjecting the extract to macroporous adsorbent resin chromatography.

Preferably, the extract is dried, redissolved with water and chromatographed on macroporous adsorbent resin.

The macroporous adsorption resin can be AB-8.

The eluent for chromatography may be an aqueous ethanol solution, the concentration of the aqueous ethanol solution is preferably 25 to 35%, more preferably 30%, and the percentage refers to volume percentage.

The volume of eluent for the chromatography may be 5 column volumes.

The chromatography may also be preceded by a depuration treatment, for example by elution with water, preferably in a volume of 1 column.

Preferably, the preparation method of the mirabilis jalapa extract comprises the following steps: mixing Mirabilis jalapa flower with ethanol water solution, sequentially performing cold soaking extraction and heating extraction to obtain extractive solution; the concentration of the ethanol water solution is 60-80%, and the percentage refers to volume percentage; the cold leaching extraction time is 12 hours; the temperature for heating and extracting is 50-70 ℃; the heating and extracting time is 1-3 h.

The invention also provides application of Mirabilis jalapa extracts as antioxidant active ingredients, whitening active ingredients and anti-aging active ingredients in cosmetics or skin care products.

Wherein the Mirabilis jalapa extract is as defined above.

The invention also provides skin external preparations, which contain 0.001-10% of mirabilis jalapa extract, wherein the percentage refers to the weight percentage (g/100mL) in the skin external preparation.

Wherein the Mirabilis jalapa extract is as defined above.

Wherein, the content of the mirabilis jalapa extract is preferably 0.0025-0.15%, such as 0.005%, 0.05% or 0.1%, and the percentage refers to the weight percentage in the skin external preparation.

When the mirabilis jalapa extract is used as an antioxidant in the skin external preparation, the content of the mirabilis jalapa extract is preferably 0.0025 to 0.0075%, more preferably 0.005%, and the percentage refers to the weight percentage in the skin external preparation.

When the mirabilis jalapa extract is used as a melanin synthesis inhibitor in the skin external preparation, the content of the mirabilis jalapa extract is preferably 0.025-0.075%, more preferably 0.05%, and the percentage refers to the weight percentage in the skin external preparation.

When the mirabilis jalapa extract is used as a skin elastic fiber hydrolysis inhibitor in the skin external preparation, the content of the mirabilis jalapa extract is preferably 0.05-0.15%, more preferably 0.1%, and the percentage refers to the weight percentage in the skin external preparation.

When the mirabilis jalapa extract is used as an up-regulator of expression of a skin aging-resistant protein in the skin external preparation, the content of the mirabilis jalapa extract is preferably 0.005 to 0.1%, for example, 0.005% or 0.1%, and the percentage refers to the weight percentage in the skin external preparation.

When the anti-skin aging protein is BMP-1, the content of the mirabilis jalapa extract is preferably 0.005%, and the percentage refers to the weight percentage in the external preparation for skin.

When the anti-skin aging protein is collagen I or collagen V, the content of the mirabilis jalapa extract is preferably 0.1%, by weight, in the skin external preparation.

In the present invention, the external preparation for skin refers to a general concept of all ingredients generally used for the external skin, and may be, for example, cosmetics or medicines. The cosmetic can be basic cosmetics, face makeup cosmetics, body makeup cosmetics, head care products and the like, and the dosage form of the cosmetic is not particularly limited and can be reasonably selected according to different purposes.

In the present invention, the external preparation for skin may further comprise other active ingredients, preferably or more of moisturizing active ingredients, antioxidant active ingredients, oil-controlling active ingredients, whitening active ingredients and anti-aging active ingredients, in an amount conventional in the art.

The external preparation for skin may further contain a vehicle or a base excipient conventionally used in the art according to various formulations and purposes, as long as the excipient is an acceptable excipient in the cosmetic or pharmaceutical field, in accordance with the general knowledge in the art. The excipient content is the content conventional in the art.

Wherein the excipient may be in the form of a water phase, an oil phase, a gel, a wax-in-water emulsion, an oil-in-water emulsion or a water-in-oil emulsion.

The skin external preparation of the present invention may further comprise step any other components conventionally used in the cosmetic field, for example, or more of preservatives, fragrances, hydrophilic active agents and lipophilic active agents.

In the present invention, the external preparation for skin may further comprise a particulate phase, and the particulate phase may be or more selected from pigments, pearling agents and fillers.

In the present invention, the form of the skin external preparation may be any suitable product form including, but not limited to, or more of aerosol type spray, cream, emulsion, solid, liquid, dispersion, foam, gel, lotion, mousse, ointment, powder, patch, pomade, hand pump type spray, stick, pack and towelette.

In a preferred embodiment of the present invention, the external skin preparation is a cosmetic water, and the external skin preparation comprises, by weight, 5% of glycerin, 3 to 6% of a polyhydric alcohol A, 1% of betaine, 0.5% of panthenol, 0.05 to 0.1% of dipotassium glycyrrhizinate, 0.15 to 0.2% of polyethylene glycol-320.5%, 0.15 to 0.2% of a surfactant A, 0.4 to 0.45% of a preservative A, 0 to 0.15% of xanthan gum, 0.03% of a fragrance, and 0.001 to 10% of the mirabilis jalapa extract, and the balance to 100% of deionized water, wherein the polyhydric alcohol A is or more selected from butylene glycol, 1, 2-pentanediol, and dipropylene glycol, the surfactant A is or more selected from polysorbate-20 and PEG-40 hydrogenated castor oil, and the preservative A is methylparaben and/or phenoxyethanol.

Wherein, the mirabilis jalapa extract is preferably a mirabilis jalapa extract. The content of the mirabilis jalapa flower extract is preferably 0.0025 to 0.15%, more preferably 0.005 to 0.1%, for example 0.005%, by weight in the skin external preparation.

In a second preferred embodiment of the present invention, the external skin preparation is a serum, and the external skin preparation comprises, by weight, 5% of glycerin, 3 to 6% of a polyol B, 1% of panthenol, 0.1% of dipotassium glycyrrhizinate, 0.1% of allantoin, 0.05 to 3.6% of a surfactant B, 0.25 to 0.45% of a preservative B, 0.2 to 0.4% of xanthan gum, 0.05% of an acrylic resin Pemulen TR-20.3% and 0.05% of a fragrance, 0.001 to 10% of a Mirabilis jalapa extract, and the balance of deionized water to 100%, wherein the polyol B is butylene glycol and/or 1, 3-propylene glycol, the surfactant B is polyglycerol-2 oleate and/or polyglycerol-10 oleate, and the preservative B is or more of propylhydroxybenzoate, methylparaben and phenoxyethanol.

According to the common knowledge in the field, the acrylic resin Pemulen TR-2 is acrylic acid (ester)/C_10-30Alkanol acrylate cross-linked polymers, produced by nuch chemical company, usa.

The xanthan gum is a saccharide, produced by fermentation of xanthomonas sp, according to common knowledge in the art, as extracellular microbial polysaccharides.

Wherein, the mirabilis jalapa extract is preferably a mirabilis jalapa extract. The content of the mirabilis jalapa flower extract is preferably 0.0025 to 0.15%, more preferably 0.005 to 0.1%, for example 0.1%, and the percentage refers to the weight percentage in the skin external preparation.

In a third preferred embodiment of the present invention, the external skin preparation is an emulsion, and the external skin preparation comprises the following components in percentage by weight: 5% of glycerol, 5% of 1, 3-propylene glycol, 1% of panthenol, 0.5% of tocopherol acetate, 2% of polydimethylsiloxane, 1652% of emulsifier A, 2-3% of oil C, 3% of isopropyl isostearate, 0.5% of cetostearyl alcohol, 0.3% of preservative C, 0.5% of xanthan gum and 0.1% of EDTA disodium; the skin external agent also comprises triethanolamine, and the content of the triethanolamine is based on adjusting the pH value of the skin external agent to 5.5-7; and 0.001% -10% of the mirabilis jalapa extract; the rest is supplemented to 100 percent by deionized water; wherein the oil C is mineral oil and/or isohexadecane; the preservative C is methyl hydroxybenzoate and/or propyl hydroxybenzoate.

The emulsifier A165 is a commercially available product containing glyceryl stearate and PEG-100 stearate, as is common in the art. The hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer is a thickening agent which is produced by Sabic corporation and has the mark of Sepinov EMT 10.

Wherein, the mirabilis jalapa extract is preferably a mirabilis jalapa extract. The content of the mirabilis jalapa flower extract is preferably 0.0025 to 0.15%, more preferably 0.005 to 0.1%, for example 0.005%, by weight in the skin external preparation.

In a fourth preferred embodiment of the present invention, the external preparation for skin is a cream, and the external preparation for skin comprises the following components in percentage by weight: 5% of glycerol, 5% of 1, 3-propylene glycol, 1% of panthenol, 0.5% of tocopherol acetate, 2% of polydimethylsiloxane, 1652% of emulsifier A, 2-8% of oil D, 3% of isopropyl isostearate, 2-3% of cetostearyl alcohol, 0.3% of preservative D, 2% of hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer and 0.1% of disodium EDTA; the skin external agent also comprises triethanolamine, and the content of the triethanolamine is based on adjusting the pH value of the skin external agent to 5.5-7; 0.001% -10% of mirabilis jalapa extract; the rest is supplemented to 100 percent by deionized water; wherein the oil D is mineral oil and/or isohexadecane; the preservative D is methyl hydroxybenzoate and/or propyl hydroxybenzoate.

Wherein, the mirabilis jalapa extract is preferably a mirabilis jalapa extract. The content of the mirabilis jalapa flower extract is preferably 0.0025 to 0.15%, more preferably 0.005 to 0.1%, for example 0.1%, and the percentage refers to the weight percentage in the skin external preparation.

On the basis of the common knowledge in the field, the above preferred conditions can be combined randomly to obtain the preferred embodiments of the invention.

The reagents and starting materials used in the present invention are commercially available.

The positive progress effects of the invention are as follows:

(1) the application discovers that the mirabilis jalapa extract, particularly the mirabilis jalapa extract, has the effects of eliminating free radical activity, inhibiting tyrosinase activity, inhibiting elastase activity and improving anti-aging protein expression in human fibroblasts, can regulate and control synthesis of collagen in dermis, has the effect of delaying aging, and particularly has the effect of improving anti-aging protein expression in human fibroblasts. Compared with a blank control, the generation rates of collagen I, collagen V and BMP1 in the fibroblasts are 133%, 125% and 150% respectively, and the method has important significance for developing skin care products or cosmetics related to anti-aging in the future.

(2) The application discovers for the first time that the mirabilis jalapa extract, particularly the mirabilis jalapa extract, can promote the generation of bone morphogenetic protein-1 (BMP-1), and has important significance for the research on the action mechanism of the mirabilis jalapa extract and the active ingredients acting on the BMP-1 target.

Detailed Description

The invention is further illustrated by the following examples , but is not intended to be limited thereby within the scope of the examples.