阅读说明：本技术 利用旋转盘系统对重金属进行定性分析和定量分析的装置及方法 (Device and method for qualitatively and quantitatively analyzing heavy metal by using rotating disc system ) 是由 朴炳贤 金炳贤 金东炫 韩秀妍 于 2018-10-25 设计创作，主要内容包括：本发明涉及一种对重金属进行定性分析和定量分析的装置及方法,更具体地,提供了一种使用旋转盘系统对重金属进行定性分析和定量分析的装置及方法。(The invention relates to devices and methods for qualitative analysis and quantitative analysis of heavy metals, and more particularly provides devices and methods for qualitative analysis and quantitative analysis of heavy metals by using a rotating disc system.)

1, apparatus for qualitative and quantitative analysis, the apparatus for qualitative and quantitative analysis comprising a rotatable platform and a plurality of microfluidic structures radially and symmetrically disposed on the rotatable platform, wherein each of the plurality of microfluidic structures comprises:

a sample injection unit into which a fluid sample containing a heavy metal is injected;

a microfluidic channel (siphon channel) which is a channel for the sample to move to the detection unit and connects the sample injection unit to the side of the detection unit;

the detection unit is coated with an organic substance capable of performing a color reaction with the heavy metal of the sample; and

a scale for measuring a color development distance,

wherein each of the plurality of microfluidic structures hold a different species of the sample,

wherein the sample is moved from the sample injection unit to the microfluidic channel and then to the detection unit by controlling rotation of the device, and

wherein the qualitative analysis is enabled by the color development reaction of the heavy metal in the detection unit, and the quantitative analysis is enabled by measuring the color development distance.

2. The device for qualitative and quantitative analysis according to claim 1, wherein the detection unit comprises a color development region coated with an organic substance capable of undergoing the color development reaction with the heavy metal of the fluid sample to develop the color of the fluid sample, and a reservoir region connecting the microfluidic channel with the color development region, wherein the reservoir region is disposed at an end of the detection unit, and the microfluidic channel is connected with a side of the reservoir region of the detection unit.

3. The apparatus for qualitative and quantitative analysis according to claim 1, wherein the sample injection unit comprises a space capable of receiving the sample and an opening for the sample to be injected.

4. The apparatus for qualitative and quantitative analysis according to claim 2, wherein the control of the rotation of the apparatus is achieved by:

rotating the device and then stopping the rotation to move the sample injected into the sample injection unit to the microfluidic channel;

rotating the device a second time to move the sample moved to the microfluidic channel to the reservoir region; and

stopping the device to allow the sample moved to the reservoir region to develop color in the detection unit.

5. The device for qualitative and quantitative analysis according to claim 4, wherein the microfluidic channel comprises partial "U" shaped tubes to accommodate the sample in the microfluidic channel after the th rotation and before the second rotation of the device.

6. The device for qualitative and quantitative analysis according to claim 4, wherein the th rotation of the device is performed at 2000RPM to less than 4000RPM for 5 to 20 seconds, and the second rotation of the device is performed at 4000RPM to 6000RPM for 3 to 10 seconds.

7. The device for qualitative and quantitative analysis according to claim 1, wherein the rotatable platform is a disc having a diameter of 12cm to 20 cm.

8. The apparatus for qualitative and quantitative analysis according to claim 1, wherein the detection unit is made of paper.

9. The method of claim 1 for determiningDevice for sexual and quantitative analysis, wherein said heavy metal contained in said sample comprises Fe²⁺、Zn²⁺、Hg²⁺、Cr⁶⁺、Ni²⁺Or Cu²⁺。

10. The apparatus for qualitative and quantitative analysis according to claim 9, wherein the organic substance pre-coated to the detection unit comprises dimethylglyoxime, bathophenanthroline, dithiooxamide, dithizone, dibenzazide or 1- (2-pyridylazo) -2-naphthol.

A method of analysis of a fluid sample containing a heavy metal using an apparatus according to any of claims 1 to 10, comprising:

(S1): injecting the sample into the sample injection unit;

(S2): controlling rotation of the device; and

(S3) performing at least of qualitative analysis and quantitative analysis on the sample developed in the detection unit.

12. The method for analyzing a fluid sample according to claim 11, wherein the injecting the sample into the sample injection unit of the step (S1) comprises:

injecting the fluid sample containing the heavy metal of a different species into each of the plurality of microfluidic structures, or

Injecting the fluid sample containing the same species of the heavy metal at different concentrations into each of the plurality of microfluidic structures.

method for analysis of fluid samples containing heavy metals using the device according to any of claims 2 to 10, comprising:

(S1): injecting the sample into the sample injection unit;

(S2): controlling rotation of the device; and

(S3) performing at least of qualitative analysis and quantitative analysis on the sample developed in the detection unit,

wherein the controlling of the rotation of the device of the step (S2) includes:

(S2-1) rotating the device th time and then stopping the th rotation to move the sample injected into the sample injection unit to the microfluidic channel;

(S2-2): rotating the device a second time to move the sample moved to the microfluidic channel to the reservoir region; and

(S2-3): stopping rotating the device to allow the sample moved to the reservoir region to develop color in the detection unit.

14. The method of analyzing a fluid sample of claim 11, wherein the performing at least of a qualitative analysis and a quantitative analysis on the sample of step (S3) comprises:

performing at least of (S3-1) and (S3-2), the (S3-1) being a qualitative analysis by the color reaction of the specimen developed in the detection unit, the (S3-2) being the quantitative analysis by measuring the color development distance.

Technical Field

The present application claims priority from korean patent application No.10-2017-0154395, filed on 20/11/2017, and korean patent application No.10-2018-0053637, filed on 10/5/2018, the disclosures of which are incorporated herein by reference.

The present invention relates to apparatuses and methods for qualitative and quantitative analysis of heavy metals, and more particularly, to apparatuses and methods for qualitative and quantitative analysis of heavy metals using a rotating disc system.

Background

, most widely used methods for detecting heavy metals are spectroscopic analysis methods such as inductively coupled plasma mass spectrometry or atomic absorption/emission spectrometry methods heavy metal detection methods based on mass spectrometry and spectrometry are accurate and have high detection limits, but are expensive and require skilled analytical techniques, making it difficult to perform heavy metal analysis in this field quickly and easily.

There is a need to develop an economical and cost-effective chromogenic-based heavy metal analysis system to replace expensive mass spectrometry and spectroscopy-based heavy metal analysis equipment, and to develop a miniaturized analysis system that can be conveniently applied to this field. In addition, there is a need to develop a system capable of performing quantitative analysis and qualitative analysis on heavy metals while shortening the analysis time by performing simultaneous detection on a plurality of heavy metals.

Further, when a fluid sample containing a heavy metal is developed in a detection unit and quantitative analysis is performed according to the development distance, a more accurate quantitative analysis method needs to be performed.

Disclosure of Invention

The present invention relates to kinds of devices for qualitative analysis and quantitative analysis, which include a rotatable platform and a plurality of microfluidic structures (microfluidic structures) radially and symmetrically disposed on the rotatable platform, each of the plurality of microfluidic structures includes a sample injection unit into which a fluid sample containing a heavy metal is injected, a microfluidic channel (siphon channel) which is a channel through which the sample can move to a detection unit and connects the sample injection unit to a end of the detection unit, the detection unit coated with an organic substance capable of undergoing a color development reaction with the heavy metal of the sample, and a scale for measuring a color development distance, each of the plurality of microfluidic structures can accommodate different kinds of the sample.

Further, in the apparatus for qualitative analysis and quantitative analysis according to the present invention, the detection unit includes a color development region coated with an organic substance capable of undergoing the color development reaction with the heavy metal of the fluid sample so that the fluid sample can be developed, and a reservoir region (reservoirrea) not coated with the organic substance, the reservoir region is disposed at an end of the detection unit, and the microfluidic channel may be connected to a side of the reservoir region of the detection unit.

Further, in the apparatus for qualitative analysis and quantitative analysis according to the present invention, the sample injection unit may include a space capable of receiving the sample and an opening into which the sample may be injected.

Further, in the device for qualitative analysis and quantitative analysis according to the present invention, the control of the rotation of the device may be achieved by rotating the device times and then stopping the times to move the sample injected into the sample injection unit to the microfluidic channel, rotating the device a second time to move the sample moved to the microfluidic channel to the reservoir region, and stopping the device to develop the sample moved to the reservoir region in the detection unit.

Further, in the device for qualitative analysis and quantitative analysis according to the present invention, the microfluidic channel may include partial "U" -shaped tubes, so that the sample may be accommodated in the microfluidic channel after the -th rotation and before the second rotation of the device.

Further, in the apparatus for qualitative analysis and quantitative analysis according to the present invention, the th rotation may be performed at 2000RPM to 4000RPM for 5 seconds to 20 seconds, and the second rotation may be performed at 4000RPM to 6000RPM for 3 seconds to 10 seconds.

Furthermore, in the device for qualitative and quantitative analysis according to the present invention, the rotatable platform is a disk and may have a diameter of 12cm to 20 cm.

Further, in the apparatus for qualitative analysis and quantitative analysis according to the present invention, the detection unit may be made of paper.

Further, in the apparatus for qualitative analysis and quantitative analysis according to the present invention, the heavy metal that may be contained in the sample may include Fe²⁺、Zn²⁺、Hg²⁺、Cr⁶⁺、Ni²⁺Or Cu²⁺。

Further, in the apparatus for qualitative analysis and quantitative analysis according to the present invention, the organic substance previously coated to the detection unit may include dimethylglyoxime, bathophenanthroline, dithiooxamide, dithizone, dibenzazide or 1- (2-pyridylazo) -2-naphthol.

Further, the present invention relates to methods of analyzing a fluid sample containing heavy metals using the apparatus for qualitative and quantitative analysis according to the present invention, the analyzing method including (S1) injecting the sample into the sample injection unit, (S2) controlling rotation of the apparatus, and (S3) performing at least of qualitative and quantitative analysis on the sample developed in the detection unit.

Further, in the method of analyzing a fluid sample containing a heavy metal according to the present invention, the injecting the sample into the sample injection unit of the step (S1) may include injecting the fluid sample containing the heavy metal of a different kind into each of the plurality of microfluidic structures, or injecting the fluid sample containing the heavy metal of the same kind at a different concentration into each of the plurality of microfluidic structures.

Further, in the method for analyzing a fluid sample containing a heavy metal according to the present invention, the controlling of the rotation of the device of the step (S2) may include (S2-1) rotating the device a th time and then stopping the th rotation to move the sample injected into the sample injection unit to the microfluidic channel, (S2-2) rotating the device a second time to move the sample moved to the microfluidic channel to the reservoir region, and (S2-3) stopping the rotation of the device to develop the sample moved to the reservoir region in the detection unit.

Further, in the method for analyzing a fluid sample containing a heavy metal according to the present invention, at least of the step (S3) of performing qualitative analysis and quantitative analysis on the sample may include performing at least of (S3-1) and (S3-2), the (S3-1) being the qualitative analysis by the color reaction of the sample developed in the detection unit, and the (S3-2) being the quantitative analysis by measuring the color development distance.

Advantageous effects

According to the device 1, 1' for qualitative analysis and quantitative analysis and the analysis method 2 of a sample using the same according to embodiments of the present invention, the detection limit of heavy metal can be improved by controlling the automatic fluid control and controlling the torque and capillary force.

According to the apparatus 1, 1 ' for qualitative and quantitative analysis and the method 2 for analyzing a sample using the same of embodiments of the present invention, it is possible to perform qualitative and quantitative analysis on several heavy metals using apparatuses 1, 1 ', according to the present invention, it is possible to perform economic and rapid qualitative/quantitative analysis of various metals, which is more economical than the conventional expensive heavy metal detector based on spectroscopy or mass spectrometry, and it is possible to shorten the analysis time, in addition, the configuration for qualitative and quantitative analysis can be integrated into miniaturized apparatuses 1, 1 ', and it can be rapidly and conveniently applied to the field requiring qualitative/quantitative analysis of heavy metals.

Furthermore, since both the channels (microfluidic channels) and the detection cells were patterned in devices, the device 1, 1' for qualitative and quantitative analysis was simple to manufacture.

Drawings

Fig. 1A shows an apparatus for qualitative analysis and quantitative analysis according to embodiments of the present invention, and fig. 1B and 1C show a microfluidic structure of the apparatus for qualitative analysis and quantitative analysis of fig. 1A.

Fig. 2A shows an apparatus for qualitative and quantitative analysis according to another embodiments of the present invention, and fig. 2B shows a microfluidic structure of the apparatus for qualitative and quantitative analysis of fig. 2A.

Fig. 3 shows each layers of the rotatable platform comprising a microfluidic structure of the device for qualitative and quantitative analysis according to fig. 1A.

Fig. 4A to 4D show each layers of the rotatable platform comprising the microfluidic structure of the device for qualitative and quantitative analysis according to fig. 2A.

Fig. 5 shows an example of a color reaction between a heavy metal ion and an organic complexing agent.

Fig. 6 shows an example of simultaneous qualitative analysis of heavy metals using the apparatus for qualitative and quantitative analysis according to the present invention.

Fig. 7A and 7B illustrate an example of quantitative analysis of heavy metals using the apparatus for qualitative and quantitative analysis according to the present invention.

Fig. 8 shows a flow chart of a method of analyzing a sample using the apparatus for qualitative and quantitative analysis according to the present invention.

Fig. 9 shows a system for qualitative and quantitative analysis, which comprises a device for qualitative and quantitative analysis according to the invention and which is capable of rotating the device for qualitative and quantitative analysis according to the invention.

Detailed Description

In an apparatus for qualitative analysis and quantitative analysis according to the present invention, which includes a rotatable platform and a plurality of microfluidic structures radially and symmetrically disposed on the rotatable platform, each of the plurality of microfluidic structures includes a sample injection unit into which a fluid sample containing a heavy metal is injected, a microfluidic channel which is a channel through which the sample can move to a detection unit and which connects the sample injection unit to a end of the detection unit, the detection unit being coated with an organic substance capable of undergoing a color development reaction with the heavy metal of the sample, and a scale for measuring a color development distance, each of the plurality of microfluidic structures can accommodate different kinds of samples, rotation of the apparatus is controlled so that the sample moves from the sample injection unit to the microfluidic channel and then to the detection unit, and qualitative analysis and quantitative analysis can be performed by measuring the color development distance by the color development reaction of the heavy metal in the detection unit.

Further, in the apparatus for qualitative analysis and quantitative analysis according to the present invention, the detection unit includes a color development region coated with an organic substance capable of developing a color reaction with a heavy metal of the fluid sample to thereby allow the fluid sample to develop a color, and a reservoir region not coated with the organic substance, the reservoir region is disposed at the end of the detection unit, and the microfluidic channel may be connected to the side of the reservoir region of the detection unit.

Further, in the apparatus for qualitative analysis and quantitative analysis according to the present invention, the sample injection unit may include a space capable of receiving the sample and an opening into which the sample may be injected.

Further, in the device for qualitative analysis and quantitative analysis according to the present invention, the control of the rotation of the device may be achieved by rotating the device th time and then stopping th time rotation to move the sample injected into the sample injection unit to the microfluidic channel, rotating the device a second time to move the sample moved to the microfluidic channel to the reservoir region, and stopping the device to develop the sample moved to the reservoir region in the detection unit.

Further, in the device for qualitative analysis and quantitative analysis according to the present invention, the microfluidic channel may include partial "U" shaped tubes so that the sample may be contained in the microfluidic channel after th rotation and before the second rotation of the device.

Further, in the apparatus for qualitative analysis and quantitative analysis according to the present invention, the th rotation may be performed at 2000RPM to less than 4000RPM for 5 seconds to 20 seconds, and the second rotation may be performed at 4000RPM to 6000RPM for 3 seconds to 10 seconds.

Furthermore, in the device for qualitative and quantitative analysis according to the present invention, the rotatable platform is a disk and may have a diameter of 12cm to 20 cm.

Further, in the apparatus for qualitative analysis and quantitative analysis according to the present invention, the detection unit may be made of paper.

In addition, the device for qualitative and quantitative analysis according to the present inventionIn the method, the heavy metal that may be contained in the sample may be Fe²⁺、Zn²⁺、Hg²⁺、Cr⁶⁺、Ni²⁺Or Cu²⁺。

Further, in the apparatus for qualitative analysis and quantitative analysis according to the present invention, the organic substance previously coated to the detection unit may include dimethylglyoxime, bathophenanthroline, dithiooxamide, dithizone, diphenylcarbazide or 1- (2-pyridylazo) -2-naphthol.

Further, in the method for analyzing a fluid sample containing heavy metals using the apparatus for qualitative and quantitative analysis according to the present invention, the method includes (S1) injecting the sample into the sample injection unit, (S2) controlling rotation of the apparatus, and (S3) performing at least of qualitative and quantitative analysis on the sample developed in the detection unit.

Further, in the method of analyzing a fluid sample containing heavy metals according to the present invention, the injecting the sample into the sample injection unit of the step (S1) may include injecting the fluid sample containing different kinds of heavy metals into each of the plurality of microfluidic structures, or injecting the fluid sample containing the same kind of heavy metals at different concentrations into each of the plurality of microfluidic structures.

Further, in the method for analyzing a fluid sample containing a heavy metal according to the present invention, the rotating of the control means of step (S2) may include (S2-1) th rotation means and then stopping th rotation to move the sample injected into the sample injection unit to the microfluidic channel, (S2-2) second rotation means to move the sample moved to the microfluidic channel to the reservoir region, and (S2-3) stopping the rotation means to develop the sample moved to the reservoir region in the detection unit.

Further, in the method for analyzing a fluid sample containing a heavy metal according to the present invention, at least of the step (S3) of performing qualitative analysis and quantitative analysis on the sample may include performing at least of (S3-1) and (S3-2), (S3-1) of performing qualitative analysis by color reaction developed in the detection unit, and (S3-2) of performing quantitative analysis by measuring a color-developed distance.

The accompanying drawings, which are included to provide a further understanding of the present invention, illustrate embodiments of the present invention and do not limit the technical scope of the present invention thereto.

Further, the same or corresponding components are denoted by the same reference numerals regardless of the drawings, and redundant description thereof will be omitted. The size and shape of each member shown may be exaggerated or minimized for convenience of explanation.

Fig. 1A shows an apparatus 1 for qualitative and quantitative analysis according to embodiments of the present invention, and fig. 1B and 1C show the microfluidic structure 20 of the rotating disk system of fig. 1A.

Referring first to fig. 1A, an apparatus 1 for qualitative and quantitative analysis includes a rotatable platform 10 and a plurality of microfluidic structures 20 disposed on the rotatable platform 10, for example, the rotatable platform 10 may be a disk, as an example, in embodiments, the size may be 12cm to 20cm in diameter, and in another embodiments, less than 12cm in diameter.

The rotatable platform 10 comprises a plurality of microfluidic structures 20 located radially and symmetrically on the rotatable platform 10. For example, the plurality of microfluidic structures 20 may include two, four, six, eight, ten, or twelve structures. In fig. 1A, six microfluidic structures 20 are shown disposed on a rotatable platform 10.

Referring to fig. 1B, each microfluidic structure 20 of the plurality of microfluidic structures 20 is shown. The microfluidic structure 20 includes a top layer (see fig. 3), a detection unit 120 coated with an organic substance capable of undergoing a color reaction with a heavy metal in a fluid sample, and a bottom layer (see fig. 3). The top layer comprises: a sample injection unit 100 into which a fluid sample containing heavy metals is injected; a microfluidic channel 110 through which the fluid sample can move to the detection unit; a portion where the detection unit 120 can be inserted; and a scale 130 for measuring the color development distance. The bottom layer is an unpatterned pressure-sensitive adhesive layer.

Each microfluidic structure 20 of the plurality of microfluidic structures 20 may contain a fluid sample containing a different type of heavy metal. For example, heavy metals that may be included in a fluid sample may include Fe²⁺、Zn²⁺、Hg²⁺、Cr⁶⁺、Ni²⁺Or Cu²⁺。

The sample injection unit 100 includes a space for receiving a fluid sample containing a heavy metal and an opening 100a into which the fluid sample can be injected, the ends of the sample injection unit 100 and the detection unit 120 may be connected to the microfluidic channel 110. furthermore, the sample injection unit 100 may include a blocking unit 100b, the blocking unit 100b prevents the sample injected through the opening 100a from directly flowing into the microfluidic channel 110, and stores the sample in an inner space of the sample injection unit 100 by using a drop (drop) of the channel, for example, since the vicinity of a rear end portion 100c of the sample injection unit 100 (at the rear end portion 100c, in the sample injection unit 100, the microfluidic channels 110 are connected to each other) has a streamlined shape, when the fluid sample injected into the sample injection unit 100 moves to the microfluidic channel 110, resistance of the fluid sample is minimized, and all the fluid sample injected into the sample injection unit 100 moves to the microfluidic channel 110.

For example, the microfluidic channel 110 may include partial "U" shaped tubes, as described below, due to the hydrophilicity of the microfluidic channel 110, after th rotation and before the second rotation of the device 1 for qualitative and quantitative analysis, the fluid sample containing the heavy metal has a channel for the fluid sample to move, and thus, the fluid sample may be accommodated in the microfluidic channel 110.

The detection unit 120 may be made of a porous hydrophilic material (e.g., paper, nitrocellulose, cotton, a silica-based sol-gel matrix, etc.), and may preferably be made of paper, in addition, the detection unit 120 may include a color development region 120a coated with an organic substance (organic ligand) capable of developing a color reaction with a heavy metal of the fluid sample so that the fluid sample may be developed, and a reservoir region 120b connecting the microfluidic channel 110 to the color development region 120 a. the reservoir region 120b may be coated with an organic substance or not coated with an organic substance. the microfluidic channel 110 is connected to the side of the reservoir region 120b of the detection unit 120. the fluid sample moving from the sample injection unit 100 to the microfluidic channel 110 during the -th rotation of the rotatable platform 10 moves from the microfluidic channel 110 to the reservoir region 120b of the detection unit 120 during the second rotation of the rotatable platform 10. at this time, the fluid sample remains in the reservoir region 120b due to the centrifugal force caused by the rotation, but does not move from the color development region 120a of the reservoir 120 to the rotatable platform 120a, when the second rotation of the rotatable platform 10 is further described below.

The scale 130 is located beside the detection unit 120 in the vicinity of the detection unit 120. For example, the scale 130 may be graduated in millimeters (mm). Alternatively, in the scale unit 130, it may be scaled in concentration units such as ppm, ppb, and the like, in addition to the length units such as mm. In the case where the scale in the scale 130 is expressed in concentration units, the scale may be expressed in concentration units obtained by substituting the color development distances of the heavy metals into the correction curve (see fig. 6).

Fig. 1C shows exemplary dimensions of the microfluidic structure 20 of the rotating disk system of fig. 1B. Exemplary dimensions of microfluidic structure 20 are not limited to those shown in fig. 1C, but may be modified or changed in accordance with various embodiments presented herein.

Fig. 2A shows an apparatus 1 ' for qualitative and quantitative analysis according to another embodiments of the present invention, and fig. 2B shows a microfluidic structure 20 ' of the rotating disk system of fig. 2A similar to the apparatus 1 for qualitative and quantitative analysis of fig. 1A, the apparatus 1 ' for qualitative and quantitative analysis of fig. 2A includes a rotatable platform 10 and a plurality of microfluidic structures 20 ' provided in the rotatable platform 10, the top layer of the rotatable platform 10 includes a sample injection unit 100 into which a fluid sample containing a heavy metal is injected, and a microfluidic channel 110, which is a channel (see fig. 4B) through which the sample can move to a detection unit, the bottom layer includes a portion into which the detection unit 120 ' can be inserted and a scale 130 for measuring a color development distance.

Meanwhile, unlike the device 1 for qualitative analysis and quantitative analysis of FIG. 1A, the device 1 ' for qualitative analysis and quantitative analysis of FIG. 2A includes an air circulation channel 140. the air circulation channel 140 is connected between the sample injection unit 100 and the other end of the detection unit 120 ', whereby the sample injection unit 100, the microfluidic channel 110, the detection unit 120 ', the air circulation channel 140, and the sample injection unit 100 are sequentially circularly connected, by introducing the air circulation channel 140, the evaporation rate of the fluid sample of the detection unit 120 ' is increased, and the moisture condensation phenomenon in the detection unit 120 ' is prevented, in addition, , with respect to the sample injection unit 100, since the air circulation channel 140 is located at the center of the disk-shaped rotatable platform 10, and the microfluidic channel (110) is disposed toward the edge of the rotatable platform 10, when the rotatable platform 10 rotates, the sample of the sample injection unit 100 is moved to the microfluidic channel 110 by centrifugal force without being moved to the air circulation channel 140. furthermore, in order to prevent the possibility of movement, a capillary valve is provided at a point where the sample injection unit 100 and the air circulation channel 140 are connected to each other, a depth of about 1mm, 8mm, to prevent the sample injection from being injected from being moved to the capillary tube valve formed by the capillary operation of the capillary tube.

Further, in the device 1 ' for qualitative and quantitative analysis of FIG. 2A, the entire detection unit 120 ' is coated with an organic substance capable of undergoing a color-developing reaction with a heavy metal of the fluid sample so that the fluid sample can be developed, and includes a reservoir region 150 provided separately from the detection unit 120 ', an end of the detection unit 120 ' is accommodated in the reservoir region 150. the reservoir region 150 is a recessed patterned region in each of the lower surface of the top layer and the upper surface of the bottom layer of the rotatable platform 10 of FIG. 4A so as to accommodate the fluid sample therein. the fluid sample accommodated in the microfluidic channel 110 during the th rotation of the rotatable platform 10 moves from the microfluidic channel 110 to the reservoir region 150 during the second rotation of the rotatable platform 10, and then is stored (i.e., trapped) in the reservoir region 150 due to a centrifugal force generated by the rotation without developing color in the detection unit 120 '.

In the device for qualitative and quantitative analysis 1 ' of fig. 2A, the end of the detection unit 120 is accommodated in the reservoir region 150 while the fluid sample is injected from the microfluidic channel 110 located in the top layer of the rotatable platform 10 to the end of the detection unit 120 inserted in the bottom layer of the rotatable platform 10, i.e., downward at the same time, in fig. 1A and 1B, the detection unit 120 is located in the top layer of the rotatable platform 10 and thus the sample is injected to the side of the detection unit 120 ' through the microfluidic channel 110. therefore, the sample injection method in the device for qualitative and quantitative analysis 1 ' of fig. 2A and 2B may more uniformly develop color on the detection unit compared to the sample injection method in the device for qualitative and quantitative analysis 1 of fig. 1A and 1B, in the description of the device for qualitative and quantitative analysis 1 ' of fig. 2A and the microfluidic structure 20 ' of fig. 2B, those of the assembly of the microfluidic assembly 1A and B described in the microfluidic assembly 20 of the microfluidic device for qualitative and 1B of fig. 1A.

FIG. 3 shows each layers of the rotatable platform 10 including the microfluidic structure 20 of FIG. 1A. the rotatable platform 10 including the microfluidic structure 20 is mainly composed of two layers. the sample injection unit 100, the microfluidic channel 110, a space in which the detection unit 120 can be inserted, and the scale 130 are located in the top layer. for example, the thickness of the top layer may be 1.0mm, and for example, the material of the top layer may include Polycarbonate (PC), Polymethylmethacrylate (PMMA), and the like. the sample injection unit 100 and the microfluidic channel 110 are disposed in the top layer, the sample injection unit 100 and the microfluidic channel 110 may be formed by using a patterning process of micro milling. various modifications and changes may be made to the portion of the top layer where the detection unit 120 is located (including a recess according to the shape of the detection unit 120) so as to enable the insertion of the detection unit 120. further, the depth of the recess may be modified and changed differently depending on the environment in which the present invention is actually implemented.

4A-4D show each layer of the rotatable platform 10 ' of FIG. 2A including the microfluidic structure 20 ', as shown in FIG. 4A, the rotatable platform 10 including the microfluidic structure 20 ' is composed primarily of three layers, each of which corresponds to a top layer (see FIG. 4B) in which the sample injection unit 100 and the microfluidic channel 110 are disposed, a bottom layer (see FIG. 4D) in which the detection unit is interposed, and a PSA (pressure sensitive adhesive) layer (see FIG. 4C) for bonding the top and bottom layers, for example, the materials of the top and bottom layers may include Polycarbonate (PC), polymethyl methacrylate (PMMA), etc. the sample injection unit 100 and the microfluidic channel 110 are disposed within the top layer, and the sample injection unit 100 and the microfluidic channel 110 may be formed by using a micro-milling patterning process, various modifications and changes may be made to the lower surface of the top layer disposed detection unit 120 ' (including recesses conforming to the shape of the detection unit 120 ') so as to enable the insertion of the detection unit 120 ', and further, the depth of recesses may be modified according to the environment in which the invention is implemented, and the bottom layer may be formed by a transparent adhesive tape 120 ' as a top layer 120, a transparent adhesive tape 120, a bottom layer, a transparent adhesive tape, a bottom layer, a transparent adhesive, a bottom layer, a sheet.

According to the device 1, 1 'for qualitative and quantitative analysis of the present invention, the rotation of the device 1 for qualitative and quantitative analysis is controlled such that the fluid sample containing heavy metal moves from the sample injection unit 100 to the microfluidic channel 110 and then to the detection unit 120, 120'. for example, after the fluid sample containing heavy metal is injected into the sample injection unit 100, when the device 1, 1 'for qualitative and quantitative analysis is rotated times at 3000RPM for 10 seconds and then stops times of rotation, the fluid sample containing heavy metal moves to the microfluidic channel 110. when the device 1, 1' for qualitative and quantitative analysis is rotated for a second time at 5000RPM, the fluid sample containing heavy metal in the microfluidic channel 110 of the top layer is injected to the liquid storage region 120b, 150 interposed in the bottom layer by centrifugal force.

The fluid sample containing the heavy metal developed on the sensing unit 120, 120 ' reacts with the reagent previously coated on the sensing unit 120, 120 ' to indicate the color related to the heavy metal, for example, an organic complexing agent may be used as an organic substance that may be previously coated on the sensing unit 120, 120 ', in embodiments, an organic substance based on a reaction list between the heavy metal ions and the organic complexing agent shown in the following table 1 may be used.

TABLE 1

Heavy metals	Form(s) of	Complexing agent (concentration)
			Nickel (Ni)2+)	Sulfates of sulfuric acid	Dimethyl glyoxime (100mM)
Iron (Fe)2+)	Sulfates of sulfuric acid	Bathophenanthroline (5mM)
			Copper (Cu)2+)	Sulfates of sulfuric acid	Dithiooxamide (20mM)
Mercury (Hg)2+)	Sulfates of sulfuric acid	Dithizone (5mM)
			Chromium (Cr)6+)	Oxide compound	Benzylcarbazine (10mM)
Zinc (Zn)2+)	Sulfates of sulfuric acid	1- (2-pyridylazo) -2-naphthol (5mM)

Fig. 5 shows the color reaction between heavy metal ions and an organic complexing agent according to table 1. In the example of fig. 5, PAN (1- (2-pyridylazo) -2-naphthol), Bphen (bathophenanthroline), DMG (dimethylglyoxime), DTO (dithiooxamide), DCB (diphenylcarbazide), and DTZ (dithizone) were used as organic complexing agents. 1% of H₂SO₄Added to Cr⁶⁺In DCB to increase Cr⁶⁺Selectivity of ion reaction to DCBAnd improve the color reaction.

The device 1, 1' for qualitative and quantitative analysis according to the invention can simultaneously treat for example Fe within 15 minutes²⁺、Zn²⁺、Hg²⁺、Cr⁶⁺、Ni²⁺Or Cu²⁺Provides a qualitative analysis of up to 25ppm of heavy metals.

Qualitative analysis of the heavy metals contained in the fluid sample may be performed using hue (hue) according to the color reaction on the detection units 120, 120'. For example, when the hue according to the color reaction is observed with the naked eye, the type of heavy metal contained in the fluid sample can be identified. In this regard, fig. 6 shows an example of simultaneous qualitative analysis of six heavy metals (100ppm) using the apparatus for qualitative and quantitative analysis 1' of fig. 2A.

In addition, the degree of color development of the fluid sample containing the heavy metal on the detection units 120, 120 ' may be quantitatively analyzed using the scales 130 of fig. 1B and 2B referring to the example of fig. 6, it can be seen that the degree of color development of the fluid sample containing the heavy metal on each of the detection units 120 ' of of the plurality of microfluidic structures (20) is different from each other, the degree of color development of the fluid sample containing the heavy metal may be measured using the scales 130, the color development distance of the corresponding fluid sample on the detection unit 120 is measured using the corresponding scales 130, the type of the heavy metal contained in the fluid sample is determined by the above-described qualitative analysis, and the quantitative analysis of the heavy metal may be performed by substituting the color development distance into the correction curve (see fig. 7A and 7B) of the heavy metal, fig. 7A shows, as examples of the quantitative analysis, the quantitative analysis using the apparatus 1 ' for qualitative analysis and quantitative analysis of fig. 2A⁶⁺FIG. 7B shows the quantitative analysis of Fe using the apparatus for qualitative and quantitative analysis 1' of FIG. 2A²⁺The case (1). For example, the numbers 1ppm, 5ppm, 10ppm, 25ppm, 50ppm and 100ppm depicted in FIG. 7A are Cr⁶⁺ methods in which a scale 130 is used to measure the corresponding Cr on six test elements 120⁶⁺Then substituting the measured color development distance into Cr⁶⁺Correction curve ofTo obtain the concentration on the x-axis of the correction curve corresponding to the degree of color development on the y-axis, so that Cr can be performed⁶⁺Quantitative analysis of (3). Fe in FIG. 7B²⁺In the case of (2), the quantitative analysis may be performed in the same manner. At this time, in the presence of Cr⁶⁺In the case of (3), the detection limit of the qualitative analysis was 1ppm, and the detection limit of the quantitative analysis was 5 ppm. In Fe²⁺In the case of (3), the detection limit of the qualitative analysis was 25ppm, and the detection limit of the quantitative analysis was 50 ppm.

Hereinafter, a method 2 of analyzing a fluid sample containing heavy metals using the apparatus 1, 1' for qualitative and quantitative analysis according to embodiments of the present invention will be described with reference to fig. 8, the steps of the method 2 of analyzing a sample according to an embodiment of the present invention are as follows:

step 1: injecting the fluid sample into the sample injection unit 100 of the apparatus for qualitative and quantitative analysis 1, 1' (S1);

step 2: controlling the rotation of the apparatus 1, 1' for qualitative and quantitative analysis (S2); and

and 3, performing at least of qualitative analysis and quantitative analysis (S3).

Step 1: sample injection unit 100 for injecting a fluid sample into a device 1, 1' for qualitative and quantitative analysis Middle (S1)

Fluid samples are injected into each of the plurality of microfluidic structures 20 of the apparatus for qualitative and quantitative analysis 1, 1 ' of each sample injection unit 100, for example, about 40 μ l of the fluid samples may be injected into each of the sample injection units 100, however, the present invention is not limited to this embodiment and the injection amount may be variously adjusted according to various environments in which the present invention is implemented, fluid samples containing different kinds of heavy metals are respectively injected into each of the plurality of microfluidic structures 20, 20 ' (S1-1) to perform qualitative and/or quantitative analysis as described below, and fluid samples containing the same kind of heavy metals at different concentrations are respectively injected into each of the plurality of microfluidic structures 20, 20 ' (S1-2) to perform qualitative and/or quantitative analysis as described below.

Step 2: controlling the rotation of the apparatus 1, 1' for qualitative and quantitative analysis (S2)

The apparatus for qualitative analysis and quantitative analysis 1, 1 ' is mounted on a system for qualitative analysis and quantitative analysis 3 (for example, a system for qualitative analysis and quantitative analysis (3) rotatable as shown in fig. 9) capable of rotating the apparatus for qualitative analysis and quantitative analysis 1, 1 ', and the apparatus for qualitative analysis and quantitative analysis 1, 1 ' is rotated. This step (S2) includes the following detailed steps:

step 2-1: th rotation of the apparatus for qualitative and quantitative analysis 1 at 2000RPM to less than 4000RPM for 5 seconds to 20 seconds and then stop th rotation to move the fluid sample containing heavy metals injected into the sample injection unit 100 located at the top layer of the microfluidic structure 20, 20' to the microfluidic channel 110 (S2-1).

Step 2-2: the apparatus for qualitative and quantitative analysis 1, 1 'is rotated for a second time at 4000RPM to 6000RPM for 3 seconds to 10 seconds to flow the fluid sample containing the heavy metal transferred to the microfluidic channel 110 at step 2-1 into the reservoir region 120b, 150 of the microfluidic structure 20, 20' (S2-2).

Step 2-3 stopping the rotation of the apparatus for qualitative and quantitative analysis 1, 1 ' so that the fluid sample containing the heavy metal is guided from the reservoir region 120b, 150 to the color development region 120a at the end of the detection cell 120/detection cell 120 ' by capillary force to develop color on the detection cell 120, 120 ' (S2-3).

Step 3 of performing at least of qualitative analysis and quantitative analysis (S3)

The qualitative analysis may be performed on the fluid sample developed on the sensing unit 120, 120 'by analyzing the color reaction on the sensing unit 120, 120' with the naked eye (S3-1); alternatively, the quantitative analysis may be performed by measuring the degree of color development of the fluid sample developed on the detection unit 120, 120' using the scale 130, and then substituting the measured value into the correction curve of the corresponding heavy metal developed on the scale (S3-2); alternatively, both qualitative and quantitative analyses may be performed (S3-1 and S3-2). Examples related thereto are described above with reference to fig. 6, 7A, and 7B.

In summary, the apparatus 1, 1 ' for qualitative and quantitative analysis according to the embodiments of the present invention includes a microfluidic structure 20 on a rotatable platform 10 (e.g., a disk), the microfluidic structure 20 having the same structure that can detect a plurality of (e.g., six) heavy metals, wherein each microfluidic structure 20 is radially and symmetrically arranged along a rotation direction of the rotatable platform 10, and each microfluidic structure 20 includes a detection unit 120, 120 ', and the detection unit 120, 120 ' is coated with an organic substance that can undergo a color reaction with the heavy metals.

According to the apparatus for qualitative and quantitative analysis 1, 1 ' and the method for analyzing a sample using the same 2 according to the embodiment of the present invention, a centrifugal force generated when the apparatus for qualitative and quantitative analysis 1, 1 ' rotates may move a fluid sample containing a heavy metal to the detection unit 120, 120 ', and the qualitative analysis may be performed by a color reaction. Further, when the device stops rotating, the fluid may be colored by the paper capillary force, and quantification may be performed by recognizing the color development distance using the scale 130 patterned on the device 1, 1' for qualitative analysis and quantitative analysis. The detection limit of heavy metals can be increased by automatic fluid control and control of torque and capillary force. The detection limit of the heavy metal ions can be increased by controlling the torque. That is, the centrifugal force and the capillary force are adjusted by controlling the rotation, so that the detection limit can be improved by controlling the color development reaction time and the color development region. Specifically, on the detecting element, when the color development speed of the sample containing the heavy metal caused by the capillary force is faster than the speed at which the heavy metal and the organic complexing agent react with each other, the sample containing the heavy metal fails to sufficiently react with the organic complexing agent and develops color on the entire detecting element. In the case where the heavy metal sample has a high concentration, there is no problem in detection due to color development, but there is a possibility that the quantitative characteristics are degraded. In the case of a low concentration heavy metal sample, color development may not occur due to insufficient reaction with the organic complexing agent of the detection unit, and detection sensitivity and detection limit may be lowered. However, according to the present invention, since a centrifugal force acts on the opposite side of the capillary force, the centrifugal force is applied to control the solution color development speed by the capillary force, so that the color development reaction can be sufficiently performed on the detection unit to increase the detection limit.

Further, the apparatus 1, 1' for qualitative and quantitative analysis and the method 2 for analyzing a sample using the same according to the embodiment of the present invention are economical and rapid in qualitative/quantitative analysis of various heavy metals. Which is more economical than conventional expensive spectroscopy or mass spectrometry based heavy metal detectors and enables shorter analysis times. Therefore, it can be rapidly and conveniently applied to the field requiring qualitative/quantitative analysis of heavy metals.

It will be understood by those skilled in the art that the technical constitution of the present invention as described above may be variously changed in form and detail without departing from the spirit and scope of the present invention. It is therefore to be understood that the above embodiments are illustrative in all respects and not restrictive. Furthermore, the scope of the invention is indicated by the appended claims rather than by the detailed description of the invention. Furthermore, all changes or modifications derived from the meaning and scope of the claims and equivalents thereof should be understood as being included in the scope of the present invention.

[ description of reference numerals ]

1. 1': device for qualitative and quantitative analysis

2: method for analyzing a sample

3: system for qualitative and quantitative analysis

10: rotatable platform

20. 20': microfluidic structures

100: sample injection unit

110: microfluidic channel

120. 120': detection unit

130: scale with a measuring device