阅读说明：本技术 甘草苷在防治作物卵菌及真菌病害中的用途 (Application of liquiritin in preventing and treating crop oomycetes and fungal diseases ) 是由 刘裴清 陈庆河 李本金 翁启勇 王荣波 于 2019-10-24 设计创作，主要内容包括：本发明公开了一种甘草苷在防治卵菌及真菌病害中的用途,其具体是利用甘草苷作为活性成分,加上农药学上可接受的辅料制成的植物杀菌剂,或直接使用甘草苷用于防治作物卵菌及真菌病害。本发明所用甘草苷来源天然,无毒副作用,对人畜安全,可作为一种低毒、有效的抑菌成分,且其不易导致病原微生物产生抗药性,可用于防治辣椒疫霉菌、隐地疫霉菌、灰葡萄孢菌和核盘菌等引起的番茄灰霉病、花菜菌核病、辣椒疫病和草霉疫病等作物病害的发生,具有巨大的潜力。(The invention discloses an application of liquiritin in preventing and treating oomycetes and fungal diseases, which is a plant bactericide prepared by using liquiritin as an active ingredient and adding auxiliary materials acceptable in agriculture and pharmacy, or directly using liquiritin to prevent and treat oomycetes and fungal diseases of crops. The liquiritin used in the invention is natural in source, has no toxic or side effect, is safe to people and livestock, can be used as a low-toxicity and effective antibacterial component, is not easy to cause pathogenic microorganisms to generate drug resistance, can be used for preventing and treating crop diseases such as tomato gray mold, cauliflower sclerotinia, pepper phytophthora blight, phytophthora blight and the like caused by phytophthora capsici, cryptophyte phytophthora crypthecae and the like, and has great potential.)

1. Application of liquiritin in preventing and treating crop oomycetes and fungal diseases.

2. The application of the plant bactericide prepared by utilizing liquiritin in preventing and treating crop oomycetes and fungal diseases.

3. The use of the plant bactericide prepared from glycyrrhizin for controlling crop oomycetes and fungal diseases according to claim 2, wherein the concentration of glycyrrhizin in the plant bactericide is 200-1600 μ g/mL.

4. The use of the plant bactericide prepared from liquiritin for controlling oomycetes and fungal diseases of crops as claimed in claim 2, wherein the plant bactericide further comprises an agriculturally and pharmaceutically acceptable auxiliary material.

5. Use according to claim 1 or 2 for controlling oomycete and fungal diseases of crops caused by phytophthora capsici, phytophthora cryptogeotrichum, botrytis cinerea and sclerotinia sclerotiorum.

Technical Field

The invention relates to application of liquiritin in preventing and treating crop oomycetes and fungal diseases.

Background

Plant diseases caused by plant pathogenic oomycetes cause great harm to the production of crops and forest trees in China, and the annual average economic loss is as high as hundreds of billions of yuan. At present, the main means for fungus and oomycete diseases in agricultural production is to use a large amount of chemical pesticides. However, since the action site of the chemical pesticide is single, the resistance is more and more obvious and shows a more and more serious trend along with the increase of the use times and the prolonging of the service life. Therefore, the search for safe alternative medicaments of high-efficiency broad-spectrum chemical bactericides is urgent, and natural products such as active ingredients of traditional Chinese medicines show great application potential in the aspect of disease control.

Radix Glycyrrhizae (Glycyrrhiza uralensis: (C)Glycyrrhizae Radix et Rhizoma) Widely distributed in northeast, Anhui, Yunnan, Xinjiang, inner Mongolia and other places of China. The composition is rich and complex, and mainly contains compounds such as flavonoid, triterpenes, coumarin and the like. The research on the pharmacological action of liquorice mainly focuses on compounds such as glycyrrhizin, total flavonoids, glycyrrhetinic acid, glycyrrhizin, polysaccharides and the like. Isoliquiritigenin, liquiritigenin, isoliquiritin and liquiritin are flavonoid components in liquorice, wherein isoliquiritin and liquiritin are flavonoid compounds. Since the 60's of the 20 th century, the pharmacological actions of licoflavone have received much attention. Many researches show that certain flavonoids compounds in liquorice not only have an inhibiting effect on various pathogens, but also can reduce the toxicity of antibacterial drugs in other chemical drugs, and are natural antibacterial drugs with great potential. The result of researches on the bacteriostatic effect of flavonoid quercetin on staphylococcus aureus and escherichia coli by Hongxiangwei and the like shows that the quercetin can achieve the bacteriostatic purpose by destroying cell wall structures and the like to cause the loss or decline of normal physiological functions of bacteria. A large number of documents show that the flavonoids have obvious inhibition effects on different bacteria and have no obvious inhibition effect on filamentous fungi, and the action research of licoflavone-liquiritin on the prevention and control of crop fungi such as botrytis cinerea, sclerotinia sclerotiorum, phytophthora capsici and phytophthora crypthecoralis and oomycete diseases is not seen at home and abroad at present.

Disclosure of Invention

Aiming at the problems in the prior art, the invention provides a new application of liquiritin.

In order to achieve the purpose, the invention adopts the following technical scheme:

the invention claims and protects the application of liquiritin in preventing and treating crop oomycetes and fungal diseases, or the application of a plant bactericide prepared from liquiritin in preventing and treating crop oomycetes and fungal diseases.

The concentration of liquiritin in the plant bactericide is 200-1600 mug/mL, and the plant bactericide also contains auxiliary materials acceptable in the agricultural pharmacy.

It can be used for preventing and treating phytophthora capsici (a)Phytophthora capsici) Phytophthora crypthecogenica (A), (B), (CPhytophthora cryptogea) Botrytis cinerea (A. cinerea)Botrytis cinerea Pers.) And Sclerotinia sclerotiorum (A), (B), (C), (Sclerotinia sclerotiorum) And the like, and particularly comprises crop epidemic diseases and fungal diseases such as tomato gray mold, cauliflower sclerotinia rot, pepper epidemic disease, phytophthora blight of grass and the like.

The disease control is mainly carried out by prevention, the principle of medicine application is adopted in advance, and preventive medicine application and early medicine application are carried out when diseases occur under the conditions of high temperature and high humidity. The method comprises mixing liquiritin or plant bactericide prepared from liquiritin with 0.02% (V/V) Tween 20 to obtain medicinal liquid, and spraying onto leaf surface to prevent dripping; the liquid medicine was diluted 10-fold (0.1 g/mL) with water at the time of actual spraying.

The invention has the following remarkable advantages:

the liquiritin is natural, has no toxic or side effect, is an effective bacteriostatic component with low toxicity, is not easy to cause pathogenic microorganisms to generate drug resistance, can be used for preventing and treating crop diseases such as tomato gray mold, cauliflower sclerotinia rot, pepper phytophthora blight, phytophthora blight and the like and fungal diseases, and has great potential.

Drawings

FIG. 1 shows the effect of different concentrations of glycyrrhizin on the growth of hyphae of phytophthora capsici and other plant pathogens, wherein A is 200, 400, 800, 1200, 1600 mug/mL (from left to right) and B is the effect of different concentrations of glycyrrhizin on the growth of phytophthora cryptophyrae hyphaeStatistical analysis of hyphal growth of bacteria, Phytophthora capsici, Botrytis cinerea and Sclerotinia sclerotiorumP< 0.05，**P<0.01。

FIG. 2 shows the effect of glycyrrhizin treatment on the hyphal morphology of Phytophthora crypthecodinii (Pm), Phytophthora capsici (Pc), Botrytis cinerea (Bc) and Sclerotinia sclerotiorum (Ss) (200 ×), wherein the left panel is a control group and the right panel is a treatment group.

FIG. 3 shows the effect of glycyrrhizin treatment on pathogenic spore production, wherein the left panel is the control group and the right panel is the treatment group.

FIG. 4 is a graph showing the effect of liquiritin treatment on Sclerotinia sclerotiorum sporulation, wherein A is a control group to which liquiritin was not added and B is a treatment group to which liquiritin was added.

FIG. 5 shows the variation of the conductivity of different mycelia after liquiritin treatment.

FIG. 6 shows SY TOX staining test (200X) of different hyphae after liquiritin treatment.

FIG. 7 shows the disease resistance of the capsicum (A), pea (B) and tomato (C) after the liquiritin treatment.

FIG. 8 is the fluorescence quantitative amplification peak diagram of Phytophthora capsici pathogenic gene after liquiritin treatment and the expression state diagram of the genes encoding CRN and 76RTP<0.05，**P<0.01。

Detailed Description

In order to make the present invention more comprehensible, the technical solutions of the present invention are further described below with reference to specific embodiments, but the present invention is not limited thereto.