阅读说明：本技术 一种抗呼吸道合胞病毒口罩及其制备方法 (Respiratory syncytial virus resistant mask and preparation method thereof ) 是由 王平 杜海涛 孙铁锋 韩莉 李玮 梁瑞雪 袁敏 李晓沫 于 2019-10-30 设计创作，主要内容包括：本发明涉及一种抗呼吸道合胞病毒口罩及其制备方法,口罩包含一个药物内芯夹层、两个无纺布外层和两个束紧带,药物内芯夹层上浸润有连翘花提取物,所述的药物内芯夹层是将连翘花粉末经超声提取、离心过滤制得的连翘花染料粉经染色在棉布内芯层而得。该口罩不仅能够有效阻挡空气中颗粒状物质,还能将有效隔离病毒经呼吸入体内的途径,适宜推广。(The invention relates to a respiratory syncytial virus resistant mask and a preparation method thereof, the mask comprises a drug inner core interlayer, two non-woven fabric outer layers and two tightening belts, wherein a forsythia suspensa flower extract is infiltrated on the drug inner core interlayer, and the drug inner core interlayer is obtained by dyeing forsythia suspensa flower dye powder prepared by ultrasonic extraction and centrifugal filtration of forsythia suspensa flower powder on a cotton cloth inner core layer. The mask can effectively block granular substances in the air, can effectively isolate viruses in the way of breathing into the human body, and is suitable for popularization.)

1. A respiratory syncytial virus resistant mask comprises a drug inner core interlayer, two non-woven fabric outer layers and two tightening belts, and is characterized in that a forsythia flower extract is soaked on the drug inner core interlayer, and the drug inner core interlayer is obtained by dyeing forsythia flower dye powder prepared by ultrasonic extraction and centrifugal filtration of forsythia flower powder on a cotton cloth inner core layer.

2. The method for preparing a respiratory syncytial virus resistant mask according to claim 1, comprising the steps of preparing an inner drug core interlayer, sewing the inner drug core interlayer to an outer non-woven fabric layer and fixing a tightening band, wherein the step of preparing the inner drug core interlayer comprises the steps of:

(1) adding mixture of ethyl acetate and ethanol into the powder of Forsythia flower in a flask, and performing ultrasonic extraction for the first time at room temperature for 20-30 min;

(2) filtering the extractive solution, adding the residue into the mixture of ethyl acetate and ethanol, and performing ultrasonic extraction at room temperature for 20-30 min;

(3) filtering the extractive solution, mixing the filtrates, and centrifuging to obtain supernatant;

(4) concentrating the supernatant liquid, sterilizing, and spray drying to obtain flos forsythiae dye powder;

(5) dyeing: taking an inner core cotton cloth layer, and dyeing in a 10-20% (owf) dyeing bath of forsythia suspensa dye powder, wherein the bath ratio is 1: 20-50, adjusting the pH value of the dye bath to 4-7, keeping the initial dyeing temperature at 30 ℃, keeping the heating rate at 1-3 ℃/min, treating at 50-80 ℃ for 30-60min, cooling to room temperature at the rate of 1-5 ℃ per minute after dyeing is finished, and finally washing and airing to obtain the dye.

3. The method for preparing a respiratory syncytial virus resistant mask according to claim 2, wherein the first ultrasonic extraction in step (1) is performed at an ultrasonic parameter of 250W at 40 kHz; the proportion of the mixture of ethyl acetate and ethanol added into the forsythia suspense flower powder is 0.1 g/ml; the volume ratio of ethyl acetate to ethanol is 1: 8, selecting 95% ethanol as ethanol.

4. The method for preparing a respiratory syncytial virus resistant mask according to claim 2, wherein the second ultrasonic extraction in step (2) is performed at an ultrasonic parameter of 250W at 40 kHz; the amount of the mixture of ethyl acetate and ethanol added in the second ultrasonic extraction is 50-70% of the first dosage, and the volume ratio of ethyl acetate to ethanol is 1: 8, selecting 95% ethanol as ethanol.

5. The method for preparing a respiratory syncytial virus resistant mask according to claim 2, wherein the rotational speed of the centrifugal machine in step (3) is 8000r/s for 10 min.

6. The method for preparing a respiratory syncytial virus resistant mask according to claim 2, wherein said dyeing process of step (5) is carried out using a low noise oscillating dyeing machine of the type XW-ZDR-25 x 12, bath ratio 1: the bath pH was adjusted to 5 with disodium hydrogen phosphate-citric acid buffer.

Technical Field

The invention relates to a preparation method of a respiratory syncytial virus resistant mask, belonging to the technical field of application of natural medicines.

Background

Respiratory syncytial virus is one of the most common pathogens causing respiratory infections, is highly contagious and can be transmitted by droplets at coughing or sneezing, and U.S. epidemiological investigations have shown that essentially all children are infected at least once by the age of 2. The susceptible population of the virus is infants and old people, severe pneumonia and bronchitis can be caused after infection, and the virus is popular in China in winter and spring every year in a large scale. At present, no reliable vaccine is available on the market, no special drug treatment is available, and the prevention method only stays on disinfection, ventilation and isolation after infection, and the risk that virus isolation is possibly incomplete exists, so that the method for blocking virus breathing by wearing an antiviral mask is a simple, economical and efficient method.

There are many kinds of masks, and the selection and treatment of the medicine have great influence on the realization of the functions of the masks. Fructus forsythiae is a plant of Oleaceae (Oleaceae), is mainly produced in Shanxi, Henan, Shanxi, Shandong and other places in China, has the effects of clearing heat and removing toxicity, eliminating stagnation and reducing swelling, has a flowering period of 3-4 months per year, is long in flowering period and large in flower quantity, and is a resource with rich sources. Research shows that the forsythia suspense flower contains rich forsythoside and forsythin and is subjected to multiple tanning, the forsythin compound has obvious RSV (respiratory syncytial virus) resisting activity, and the tanning has obvious oxidation resistance and free radical scavenging capacity. Therefore, the compound has the potential of preventing and treating RSV. There are many methods for extracting and processing drugs, and the microwave extraction of drugs is different from the traditional extraction method in the aspects of mechanism and process, and can be summarized into three aspects: in the microwave extraction process, the microwave irradiation can realize the simultaneous heating inside and outside the extraction system (solvent and sample), and after the system absorbs microwave energy, the movement of drug molecules is accelerated, and the temperature is rapidly increased; meanwhile, molecules in the drug cells absorb microwave energy, so that the temperature in the cells is rapidly increased, the cells are instantaneously ruptured, and the dissolution and diffusion of drug components are accelerated; in addition, the solute molecules also absorb microwave energy, and the movement is accelerated, thereby rapidly dissolving into the solvent. Therefore, the microwave extraction has the characteristics of high speed, high efficiency, energy conservation, environmental protection and the like, is known as a green extraction technology, and accords with the development direction of green chemistry. The efficiency of microwave extraction is influenced by various factors, such as the type of solvent, the amount of solvent used, the microwave power, the extraction temperature, the extraction time, the nature and the granularity of the medicine, and the like, and the factors have mutual influence. How to prepare a mask with a good antiviral effect by using natural medicines is the key point.

Disclosure of Invention

The invention aims to overcome the defects and provide the respiratory syncytial virus resistant mask and the preparation method thereof.

The technical scheme adopted by the invention is as follows:

a respiratory syncytial virus resisting mask comprises a medicine inner core interlayer, two non-woven fabric outer layers and two tightening belts, wherein a forsythia suspensa flower extract is soaked on the medicine inner core interlayer, and the medicine inner core interlayer is obtained by dyeing forsythia suspensa flower dye powder which is prepared by carrying out ultrasonic extraction and centrifugal filtration on forsythia suspensa flower powder on a cotton cloth inner core layer.

The preparation method of the respiratory syncytial virus resistant mask comprises the steps of preparation of a medicine inner core interlayer, sewing of the medicine inner core interlayer and an outer layer of non-woven fabric and fixing of a tightening belt, wherein the preparation of the medicine inner core interlayer comprises the following steps:

(1) adding mixture of ethyl acetate and ethanol into the powder of Forsythia flower in a flask, and performing ultrasonic extraction for the first time at room temperature for 20-30 min;

(2) filtering the extractive solution, adding the residue into the mixture of ethyl acetate and ethanol, and performing ultrasonic extraction at room temperature for 20-30 min;

(3) filtering the extractive solution, mixing the filtrates, and centrifuging to obtain supernatant;

(4) concentrating the supernatant liquid, sterilizing, and spray drying to obtain flos forsythiae dye powder;

(5) dyeing: taking an inner core cotton cloth layer, and dyeing in a 10-20% (owf) dyeing bath of forsythia suspensa dye powder, wherein the bath ratio is 1: 20-50, adjusting the pH value of the dye bath to 4-7, keeping the initial dyeing temperature at 30 ℃, keeping the heating rate at 1-3 ℃/min, treating at 50-80 ℃ for 30-60min, cooling to room temperature at the rate of 1-5 ℃ per minute after dyeing is finished, and finally washing and airing to obtain the dye.

The first ultrasonic extraction in the step (1) in the preparation method is carried out, and the ultrasonic parameters are 250W and 40 kHz; the proportion of the mixture of ethyl acetate and ethanol added into the forsythia suspense flower powder is 0.1 g/ml; the volume ratio of ethyl acetate to ethanol is 1: 8, the ethanol is preferably 95% ethanol.

Performing ultrasonic extraction for the second time in the step (2), wherein ultrasonic parameters are 250W and 40 kHz; the amount of the mixture of ethyl acetate and ethanol added in the second ultrasonic extraction is 50-70% of the first dosage, and the volume ratio of ethyl acetate to ethanol is 1: 8, the ethanol is preferably 95% ethanol.

And (4) centrifuging for 10min at the rotating speed of 8000r/s of the centrifugal centrifuge in the step (3).

The dyeing process in the step (5) is preferably carried out by using an XW-ZDR-25X 12 type low-noise oscillation dyeing machine, and the bath ratio is 1: the bath pH was adjusted to 5 with disodium hydrogen phosphate-citric acid buffer.

The beneficial effects of the invention are as follows:

the preparation method adopts ultrasonic extraction, has the characteristics of high speed, high extraction rate, low temperature, solvent saving and the like, is not limited by the polarity and molecular mass of components, and has small influence on the structure and activity of the extract; the centrifugal treatment can promote the effective components to exert the curative effect, and the micromolecule active substances are remained in the centrifugal liquid, and the large impurities in the extracted stock solution are removed after centrifugation, so that the therapeutic index of the liquid medicine is improved compared with that of the stock solution; the extract liquid in dyeing belongs to natural pigment, the main component is flavonoid compound, the extract liquid is light yellow powder at room temperature, the extract liquid can be easily dissolved in warm water (40-80 ℃), the extract liquid is suitable for a medicine inner sandwich layer, respiratory airflow passes through the extract liquid when the extract liquid is used, the extract liquid can be replaced frequently, and mordant does not need to be added.

The medicine inner core interlayer is soaked with the prepared forsythia suspense flower extract which contains rutin, forsythoside, phillyrin and the like which are antiviral active substances of the forsythia suspense flower, the extract has the effect of resisting respiratory syncytial virus, and particularly, the effect of an extract obtained after centrifugation on the respiratory syncytial virus is obviously better than that of an extraction stock solution and a filter membrane solution. The mask adopts the interlayer design, so that the filter layer can be conveniently replaced in the later period; meanwhile, the method makes up the defects of the existing method for preventing the respiratory syncytial virus, not only can effectively block granular substances in the air, but also can effectively isolate the virus and inhale the virus into the human body through breath, and is suitable for popularization.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.