阅读说明：本技术 一种干细胞抗衰老试剂制备方法 (Preparation method of stem cell anti-aging reagent ) 是由 江晔 程志强 于 2021-08-30 设计创作，主要内容包括：本发明公开了一种干细胞抗衰老试剂制备方法,包括以下步骤：选取羊的脐带血,并往脐带血中加入抗凝剂,然后采用氯化钠溶液稀释羊的脐带血,倒入活性提取液中,充分震荡,制得细胞悬浮液,对细胞悬浮液采用磷酸盐缓冲液进行冲洗,制得干细胞提取液,将干细胞提取液在冰浴中进行超声破碎,并使用高速离心机离心干细胞提取液,制得干细胞精华液,往干细胞精华液中添加六胜肽原液、视黄醇溶液、和植物提取物从而制得试剂。本发明利用干细胞制成的抗衰老试剂,通过在干细胞制成的抗衰老试剂中加入六胜肽原液、视黄醇溶液和植物精华,促进了胶原蛋白的生长,同时添加植物成分天然安全、环保,提高了试剂抗衰老功效,满足了人们的需求。(The invention discloses a preparation method of a stem cell anti-aging reagent, which comprises the following steps: the preparation method comprises the steps of selecting sheep umbilical cord blood, adding an anticoagulant into the umbilical cord blood, diluting the sheep umbilical cord blood by using a sodium chloride solution, pouring the diluted sheep umbilical cord blood into an active extracting solution, fully shaking to obtain a cell suspension, washing the cell suspension by using a phosphate buffer solution to obtain a stem cell extracting solution, carrying out ultrasonic crushing on the stem cell extracting solution in an ice bath, centrifuging the stem cell extracting solution by using a high-speed centrifuge to obtain a stem cell essence, and adding a hexapeptide stock solution, a retinol solution and a plant extract into the stem cell essence to obtain the reagent. The anti-aging reagent prepared from the stem cells is added with the hexapeptide stock solution, the retinol solution and the plant essence, so that the growth of collagen is promoted, meanwhile, the plant components are added, the anti-aging effect of the reagent is improved, and the requirements of people are met.)

1. The preparation method of the stem cell anti-aging reagent is characterized by comprising the following steps:

the method comprises the following steps: selecting sheep umbilical cord blood, adding an anticoagulant into the umbilical cord blood, mixing vitamin C into a sodium chloride solution, and diluting the sheep umbilical cord blood by using the sodium chloride solution mixed with the vitamin C;

step two: pouring the diluted cord blood into the active extracting solution, and then fully shaking to prepare a cell suspension;

step three: carrying out centrifugal separation on the cell suspension, and then repeatedly washing by adopting a phosphate buffer solution to prepare a stem cell extracting solution;

step four: carrying out ultrasonic crushing on the stem cell extracting solution in an ice bath, centrifuging the stem cell extracting solution by using a high-speed centrifuge, and taking supernate to prepare stem cell essence;

step five: adding the hexapeptide stock solution into the stem cell essence solution, and fully stirring;

step six: adding a retinol solution into the stem cell essence, and fully stirring;

step seven: processing folium Eucalypti Globueli, semen Maydis, radix et caulis Opuntiae Dillenii, herba Houttuyniae, Ginseng radix Rubri, semen euryales and fructus Pyracanthae, and extracting plant materials;

step eight: mixing Eucalyptus globulus Labill leaf extract, semen Maydis extract, radix et caulis Opuntiae Dillenii extract, herba Houttuyniae extract, Ginseng radix Rubri extract, semen euryales extract and fructus Pyracanthae extract with deionized water, and heating to obtain emulsified product;

step nine: adding the prepared emulsified product into stem cell essence, fully stirring, and performing vacuum degassing to obtain the reagent.

2. The method for preparing the stem cell anti-aging agent according to claim 1, wherein the volume ratio of the sheep umbilical cord blood to the sodium chloride solution in the first step is 1.1: 1, the content of the maintainer C in the sodium chloride solution is 100MG/L, and the first step needs to be carried out in an environment of 2-6 ℃.

3. The method for preparing the stem cell anti-aging agent according to claim 1, wherein the number of times of washing with the sodium phosphate buffer solution in step three is 2-4.

4. The method for preparing the stem cell anti-aging reagent according to claim 1, wherein in the fourth step, the stem cell extracting solution is centrifuged for 10 minutes, the temperature is controlled at 2-6 ℃, and the rotating speed of a height centrifuge is 11000R.

5. The method for preparing the stem cell anti-aging agent according to claim 1, wherein the stock solution of the hexapeptide in the fourth step is a small molecular protein consisting of 6 amino acids, and the retinol solution in the fifth step is one of the forms of vitamin A.

6. The method for preparing the stem cell anti-aging reagent according to claim 1, wherein in the sixth step, the eucalyptus globulus leaf, the corn, the cactus, the houttuynia cordata, the red ginseng, the gorgon fruit and the pyracantha fortuneana fruit are all extracted by removing impurities, washing, drying in the sun and mashing, and the cactus needs to be sliced before removing the impurities, washing, drying in the sun and mashing.

Technical Field

The invention relates to the technical field of biomedicine, in particular to a preparation method of a stem cell anti-aging reagent.

Background

Aging refers to the phenomenon that the physiological and psychological adaptive capacity of the body to the environment is gradually reduced and gradually dies. Senescence is a corollary of the combined effects of many pathological, physiological and psychological processes, the biological psychological process of the final stages of growth and development of an individual. Stem cells are a class of cells that have unlimited or immortal self-renewal capacity, capable of producing at least one type of highly differentiated progeny cells. The function of the stem cell population is to control and maintain the regeneration of cells.

Along with the continuous improvement of living standard, people also attach more importance to having a healthy health, wherein the aging of body skin is a problem that a plurality of beauty lovers attach more importance, and the expression of the phenomenon of skin excessive aging is: the skin care products at present only have the moisturizing effect, so that the requirements of people cannot be met

Disclosure of Invention

The invention aims to provide a preparation method of a stem cell anti-aging reagent, which aims to solve the problems in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme: a preparation method of a stem cell anti-aging reagent comprises the following steps:

the method comprises the following steps: selecting sheep umbilical cord blood, adding an anticoagulant into the umbilical cord blood, mixing vitamin C into a sodium chloride solution, and diluting the sheep umbilical cord blood by using the sodium chloride solution mixed with the vitamin C;

step two: pouring the diluted cord blood into the active extracting solution, and then fully shaking to prepare a cell suspension;

step three: carrying out centrifugal separation on the cell suspension, and then repeatedly washing by adopting a phosphate buffer solution to prepare a stem cell extracting solution;

step four: carrying out ultrasonic crushing on the stem cell extracting solution in an ice bath, centrifuging the stem cell extracting solution by using a high-speed centrifuge, and taking supernate to prepare stem cell essence;

step five: adding the hexapeptide stock solution into the stem cell essence solution, and fully stirring;

step six: adding a retinol solution into the stem cell essence, and fully stirring;

step seven: processing folium Eucalypti Globueli, semen Maydis, radix et caulis Opuntiae Dillenii, herba Houttuyniae, Ginseng radix Rubri, semen euryales and fructus Pyracanthae, and extracting plant materials;

step eight: mixing Eucalyptus globulus Labill leaf extract, semen Maydis extract, radix et caulis Opuntiae Dillenii extract, herba Houttuyniae extract, Ginseng radix Rubri extract, semen euryales extract and fructus Pyracanthae extract with deionized water, and heating to obtain emulsified product;

step nine: adding the prepared emulsified product into stem cell essence, fully stirring, and performing vacuum degassing to obtain the reagent.

Preferably, the volume ratio of the sheep umbilical cord blood to the sodium chloride solution in the first step is 1.1: 1, the content of the maintainer C in the sodium chloride solution is 100MG/L, and the first step needs to be carried out in an environment of 2-6 ℃.

Preferably, the number of times of washing with the sodium phosphate buffer in the third step is 2-4.

Preferably, in the fourth step, the stem cell extracting solution is centrifuged for 10 minutes, the temperature is controlled to be 2-6 ℃, and the rotating speed of the height centrifuge is 11000R.

Preferably, the stock solution of the hexapeptide in the fourth step is a small molecular protein consisting of 6 amino acids, and the retinol solution in the fifth step is one of the forms of vitamin A.

Preferably, in the sixth step, the eucalyptus globulus leaf, the corn, the cactus, the houttuynia cordata, the red ginseng, the gorgon fruit and the pyracantha fortuneana fruit are subjected to impurity removal, cleaning, sun drying and mashing to obtain the extract, and the cactus needs to be sliced before the impurity removal, cleaning, sun drying and mashing.

The invention has the technical effects and advantages that:

(1) the anti-aging reagent prepared from the stem cells is added with the hexapeptide stock solution, the retinol solution and the plant essence, so that the growth of collagen is promoted, meanwhile, the plant components are added, the anti-aging effect of the reagent is improved, and the requirements of people are met.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The invention provides a preparation method of a stem cell anti-aging reagent, which comprises the following steps:

the method comprises the following steps: selecting sheep umbilical cord blood, adding an anticoagulant into the umbilical cord blood, adding the anticoagulant for preventing the sheep umbilical cord blood from solidifying, mixing vitamin C into a sodium chloride solution, wherein the vitamin C is an important coenzyme and antioxidant in vivo, has a reversible redox effect, participates in the action of an intracellular respiratory chain, maintains the normal energy metabolism of tissue cells and organs, and is favorable for the complete survival, proliferation and development of the cells; the key component of the intercellular substance is collagen, vitamin C participates in the synthesis of the collagen and is beneficial to healing of human body wounds, the content of the vitamin C in the sodium chloride solution is maintained to be 100MG/L, the sodium chloride solution mixed with the vitamin C is adopted to dilute the cord blood of sheep, and the volume ratio of the cord blood of sheep to the sodium chloride solution is 1.1: 1, the above processes are carried out in an environment of 2-6 ℃;

step two: pouring the diluted cord blood into the active extracting solution, and then fully shaking to prepare a cell suspension;

step three: carrying out centrifugal separation on the cell suspension, then repeatedly washing by adopting a phosphate buffer solution, wherein the washing frequency by adopting a sodium phosphate buffer solution is 2-4 times, so as to prepare a stem cell extracting solution;

step four: ultrasonically crushing the stem cell extracting solution in an ice bath, centrifuging the stem cell extracting solution by using a high-speed centrifuge, wherein the centrifuging time of the stem cell extracting solution is 10 minutes, the temperature is controlled at 2-6 ℃, the rotating speed of the high-speed centrifuge is 11000R, and taking supernate to prepare stem cell essence;

step five: adding a hexapeptide stock solution into the stem cell essence, and fully stirring, wherein the hexapeptide stock solution is micromolecular protein consisting of 6 amino acids and can promote the growth of collagen cells and fibroblasts;

step six: adding a retinol solution into the stem cell essence, and fully stirring, wherein the retinol solution is one of the forms of vitamin A, and the retinol can prevent the collagen of the dermis from being decomposed and promote the synthesis of the collagen of the dermis;

step seven: the eucalyptus globulus leaf, the corn, the cactus, the houttuynia cordata, the red ginseng, the gorgon fruit and the pyracantha fortuneana fruit are processed to extract plant raw materials, the eucalyptus globulus leaf, the corn, the cactus, the houttuynia cordata, the red ginseng, the gorgon fruit and the pyracantha fortuneana fruit are subjected to impurity removal, cleaning, sun drying and mashing to obtain extracts, the cactus needs to be sliced before impurity removal, cleaning, sun drying and mashing, the eucalyptus globulus leaf has excellent curative effects on burn and scald of human beings, cellulitis, mastitis, paddy field skin, skin eczema and other common skin diseases, the corn contains rich vitamin B, the integrity of cell skin can be guaranteed, the cactus extract can remove wrinkles, the skin is more thoroughly and smooth, the skin elasticity and moisture can be increased, the regeneration and repair of cells can be promoted, the balance regulation effect on various nutrients can be realized, and the houttuynia cordata variety of minerals and trace elements are contained in the houttuynia cordata, in metabolism, the microelements can promote local cell proliferation, especially have obvious effect on gliadin, the red ginseng has the effect of enhancing cell regeneration capacity, the gorgon fruit contains vitamin C, vitamin B group, protein paper and the like, which are beneficial to anti-aging, and the pyracantha fortuneana fruit can improve internal circulation and promote metabolism;

step eight: mixing Eucalyptus globulus Labill leaf extract, semen Maydis extract, radix et caulis Opuntiae Dillenii extract, herba Houttuyniae extract, Ginseng radix Rubri extract, semen euryales extract and fructus Pyracanthae extract with deionized water, and heating to obtain emulsified product;

step nine: adding the prepared emulsified product into stem cell essence, fully stirring, and performing vacuum degassing to obtain the reagent.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.