阅读说明：本技术 一种金纳米团簇-金纳米棒新型免疫传感器对睾酮的检测方法 (Method for detecting testosterone by using gold nanocluster-gold nanorod novel immunosensor ) 是由 丁伟华 孙斐 顾亚云 赵喜 李文清 于 2019-10-09 设计创作，主要内容包括：本发明公开了一种金纳米团簇-金纳米棒新型免疫传感器对睾酮的检测方法,包括：步骤1、金纳米团簇(荧光供体)和金纳米棒(荧光受体)的制备；步骤2、金纳米团簇(荧光供体)和金纳米棒(荧光受体)分别修饰连接；步骤3、对睾酮分子的检测,金纳米团簇(荧光供体)与睾酮修饰连接,金纳米棒(荧光受体)与睾酮抗体修饰连接；该发明基于FRET机理设计新型免疫传感器,选用具有生物相容性、光学性能好、易于合成的金纳米团簇为荧光供体,选用LSPR峰可调的金纳米棒为荧光受体,将睾酮、睾酮抗体分别与金纳米团簇、金纳米棒进行连接,通过调控合成条件及进行功能修饰,提高两者间的FRET效率,实现对睾酮分子的特异性、高效性检测。(The invention discloses a method for detecting testosterone by a gold nanocluster-gold nanorod novel immunosensor, which comprises the following steps: step 1, preparing gold nanoclusters (fluorescence donors) and gold nanorods (fluorescence acceptors); step 2, respectively modifying and connecting the gold nanoclusters (fluorescence donors) and the gold nanorods (fluorescence acceptors); step 3, detecting testosterone molecules, namely modifying and connecting the gold nanoclusters (fluorescence donors) and testosterone, and modifying and connecting the gold nanorods (fluorescence acceptors) and testosterone antibodies; the invention designs a novel immunosensor based on FRET mechanism, selects gold nanoclusters with biocompatibility, good optical performance and easy synthesis as fluorescence donors, selects gold nanorods with adjustable LSPR peak as fluorescence acceptors, connects testosterone and testosterone antibodies with the gold nanoclusters and the gold nanorods respectively, improves FRET efficiency between the two by regulating synthesis conditions and performing functional modification, and realizes specificity and high-efficiency detection of testosterone molecules.)

1. A method for detecting testosterone by a gold nanocluster-gold nanorod novel immunosensor comprises the following steps: step 1, preparing gold nanoclusters (fluorescence donors) and gold nanorods (fluorescence acceptors); step 2, respectively modifying and connecting the gold nanoclusters (fluorescence donors) and the gold nanorods (fluorescence acceptors); step 3, the detection of testosterone molecules is characterized in that: the gold nanoclusters (fluorescence donors) are connected with testosterone modification, and the gold nanorods (fluorescence acceptors) are connected with testosterone antibody modification; the step 3 is as follows:

1) mixing, namely mixing gold nanocluster-testosterone solution with the same volume with testosterone antibody-gold nanorod solution for reaction to prepare mixed solution A;

2) testing the fluorescence property of the mixed solution A, detecting the change of the fluorescence property under different conditions, selecting the corresponding detection condition when the absolute value of the change of the fluorescence emission intensity is maximum, and determining the detection condition as the optimal condition;

3) and (3) detecting, namely mixing the mixed solution A with a testosterone molecular solution (to-be-detected solution), detecting under the optimal condition in the step 2), and obtaining the concentration of testosterone according to the change of fluorescence emission intensity and ultraviolet visible absorption intensity.

2. The method for detecting testosterone by using the gold nanocluster-gold nanorod novel immunosensor as claimed in claim 1, wherein the method comprises the following steps: the preparation method of the gold nanocluster (fluorescence donor) comprises the following steps:

step A1: adding HAuCl₄Diluting with 10.0mM water, adding Glutathione (GSH) (10.0mM), and adding HAuCl into the above components₄The volume ratio of water to glutathione is 1 (1-2) to 0.5-1, and the reaction is carried out for 30-70 minutes under the stirring of 1200rpm at the temperature of 90 ℃ to obtain a reaction solution a 1;

step A2: centrifuging the reaction solution a1 at 11000rpm for 20-40 min to obtain supernatant a 2;

step A3: dialyzing the supernatant a2 with a dialysis membrane for 40-60 hours to remove unreacted organic matters to obtain gold nanocluster liquid a 3;

step A4: and (3) freeze-drying the gold nanocluster liquid a3 to obtain the gold nanoclusters (fluorescence donors).

3. The method for detecting testosterone by using the gold nanocluster-gold nanorod novel immunosensor as claimed in claim 2, wherein the method comprises the following steps: the preparation method of the gold nanorod (fluorescence acceptor) comprises the following steps:

step B1: ice cold sodium oleate solution is added to HAuCl prepared in CTAB solution under stirring₄In solution, the solution turned yellow to brown, indicating the formation of gold species b 1;

step B2: gold seed b1 was aged for 1-3 hours to allow unreacted NaBH₄Hydrolyzing, and amplifying the growth process in proportion to obtain gold nanorods;

step B3: mixing 0.1mM CTAB solution, 4mM silver nitrate solution, 25mM HAuCl₄The aqueous solution and the 1mM HCl solution were sequentially added to mix, and 0.0788mM antibody was added theretoAscorbic acid as a reducing agent, and homogenizing the mixture by stirring to obtain a mixture b 3; CTAB solution, silver nitrate solution, and HAuCl₄The volume ratio of the aqueous solution, the HCl solution and the ascorbic acid is 100: (5-10): (1-2): 0.6-2): 0.5-1.2);

step B4: adding 0.5-1.5% of gold seeds in volume ratio into the mixture b3, and standing the whole solution for 36 hours to obtain gold nanorods.

4. The method for detecting testosterone by using the gold nanocluster-gold nanorod novel immunosensor as claimed in claim 3, wherein the testosterone detection method comprises the following steps: the gold nanoclusters (fluorescence donors) and testosterone modification connection are as follows:

step C1: adding the gold nanocluster solution into a mixed solution containing PBS (0.01mol/L, pH 7.4) and 4mg/mL EDC to prepare a mixed solution c1, and stirring the mixed solution c1 for 5 minutes and then for 20 minutes to activate free carboxylic acid groups on the gold nanoclusters;

step C2: adding 0.15mg/mL sulfo-NHS to the mixed solution c1 to prepare a mixed solution c2, and stirring the mixed solution c2 for 20 minutes;

step C3: adding 0.01mg/mL testosterone solution into the mixed solution c2 to obtain a mixed solution c3, reacting at 37 ℃ in the dark for 2 hours, and storing the mixed solution c3 at 4 ℃; the volume ratio of the gold nanocluster solution, the PBS, the EDC, the sulfo-NHS and the testosterone solution is 1: 3:(0.8-1.2): (0.04-0.08): (0.01-0.02);

step C4: performing tests through a fluorescence spectrum and an ultraviolet visible absorption spectrum to obtain the spectrum overlapping of the gold nanocluster-testosterone and testosterone antibody-gold nanorod system; modifying and connecting the gold nanoclusters (fluorescence donors) and testosterone antibodies to obtain a gold nanocluster-testosterone solution;

the gold nanorods (fluorescent receptors) are modified and connected with testosterone antibodies as follows:

step D1: adding 10 mug/mL testosterone antibody to 47.8 mug/mL gold nanorods under magnetic stirring; after stirring for 10 minutes, 3% PEG20000 is added as a stabilizer to obtain a mixture d 1; the volume ratio of the testosterone antibody, the gold nanorods and the PEG20000 of each component is as follows: 1:(50-85): (0.13-0.4);

step D2: and stirring the mixture d1 for 15 minutes and storing at 4 ℃ to realize the modified connection of the gold nanorods and the testosterone antibody, so as to obtain a gold nanorod and testosterone antibody solution.

Technical Field

The invention belongs to the technical field of hormone detection and analysis, and particularly relates to a method for detecting testosterone by a gold nanocluster-gold nanorod novel immunosensor.

Background

Testosterone is secreted from male testis or female ovary, adrenal gland also secretes a small amount of testosterone, has the effects of maintaining muscle strength and quality, maintaining bone density and strength, refreshing and improving physical performance, and is a main endogenous androgen. Decreased testosterone concentrations may cause a variety of disorders such as anxiety, irritability, sexual dysfunction, memory loss, abdominal obesity, and the like; however, excessive testosterone levels can also cause a variety of diseases such as precocious puberty, adrenal disorders, testicular disorders, and the like. In addition, the abuse of testosterone as a sex hormone stimulant during sporting events has also been a concern. Therefore, monitoring testosterone levels in biological fluids is of practical interest for clinical, biochemical and motor endocrinological studies.

The traditional detection means, such as electrochemical analysis, atomic spectrometry, liquid chromatography, radioactive labeling and the like, have the defects of complex operation, poor sensitivity and the like, so that the application of the detection on testosterone molecules is greatly limited. With the continuous development of bioengineering technology, biosensors have been gradually applied to the fields of environmental detection, food, industry, and clinical medicine. The immunosensor is an emerging biosensor, and based on the principle of specific binding of antigen and antibody, the high specificity, sensitivity and stability of the identified substance are favored by a plurality of researchers, and the emergence of the immunosensor greatly changes the traditional immunoassay technology. The immunosensor integrates the biosensing technology and the traditional immunoassay technology, integrates the advantages of the biosensing technology and the traditional immunoassay technology, improves the detection sensitivity and the detection accuracy, reduces the analysis time, simplifies the determination process, is easy to realize automation, and has potential application prospect.

In recent years, immunosensors based on Surface Plasmon Resonance (SPR) have attracted research interest in the analysis and monitoring of biological and chemical substances. However, such immunosensors also have a number of drawbacks, such as poor sensitivity for detecting small molecule substances. Although Huang and Komatsu et al have successfully used different nanoparticles to improve the response signals and sensitivity of SPR-based immunosensors to protein molecules and oligoglycoside nucleic acids, such sensors have not made significant progress in the study of the measurement of small molecules.

Fluorescence Resonance Energy Transfer (FRET) is a sensitive spectroscopic detection technique, and is a non-radiative energy transfer process of Fluorescence resonance energy from an excited state fluorophore as a donor to a ground state acceptor through a long-range dipole-dipole interaction, and can be used for studying structural changes of molecules in a homogeneous system. Among them, one of the most commonly used techniques is to combine a fluorescence donor and an acceptor with a biomolecule (e.g., an antigen and an antibody) respectively to allow specific interaction, thereby becoming an "artificial immunosensor". Therefore, an immunosensor based on the FRET mechanism has significant specificity for a target molecule, and is considered to be a very sensitive detection method in the aspect of biological analysis. In such immunosensors, the sensitivity of the detection method depends primarily on the FRET efficiency between the fluorescent donor and acceptor. The following two factors are the main factors for determining FRET efficiency: (1) the degree of overlap of the fluorescence donor emission spectrum and the acceptor absorption spectrum; (2) the spatial distance between the donor molecule and the acceptor molecule. Generally, the optimal distance between the donor molecule and the acceptor molecule for the FRET process is 2-9 nm. Therefore, it is necessary to consider shortening the distance between the donor and the acceptor as much as possible and increasing the overlap of the emission spectrum of the donor and the absorption spectrum of the acceptor in the experimental design.

At present, immunosensors based on the FRET mechanism have been widely reported for the detection of biomolecules. For example, Wang et al have designed a homogeneous immunosensor for the detection of Human Serum Albumin (HSA) based on the FRET mechanism, wherein HSA antigens and antibodies are combined with two DNA segments, respectively, to allow specific interactions, thereby achieving rapid and accurate detection of human serum albumin in serum, urine and saliva. Bazan et al, which connects fluorescein to the end of dsDNA as a fluorescence donor, makes it have FRET effect with organic polymer molecules, and explores the cascade energy transfer process through fluorescence decay kinetics and fluorescence anisotropy tests. In the above immunosensor, both an organic dye and a fluorescent protein are used as a fluorescence donor or acceptor. However, such immunosensors suffer from a number of drawbacks, such as fixed emission/absorption wavelengths, poor stability, photobleaching, and the like. In order to overcome the above disadvantages, researchers have considered quantum dot materials as fluorescence donors because quantum dots have high quantum yields, strong photostability, narrow emission peaks, and wide excitation wavelength ranges. For example, Cai et al use CdTe quantum dots as fluorescence donors, quantum dots-lysozyme and lysozyme antibody-gold nanomaterials as immunosensors, and detect lysozyme molecules based on FRET mechanism, with a detection limit of 33.43 ng/mL. However, the potential human toxicity and cytotoxicity of quantum dot materials are two major problems for their in vitro and in vivo applications. Therefore, the selection of fluorescent donor and acceptor with good fluorescence performance, good stability, easy synthesis, low toxicity or no toxicity is important for designing fluorescence immunosensor based on FRET mechanism.

Disclosure of Invention

Based on the defects of the prior art, the technical problem to be solved by the invention is to provide a method for detecting testosterone by using a gold nanocluster-gold nanorod novel immunosensor, wherein the gold nanocluster which has biocompatibility and good optical performance and is easy to synthesize is used as a fluorescence donor, the gold nanorod with an adjustable LSPR peak is used as a fluorescence acceptor, testosterone and testosterone antibodies are respectively connected with the gold nanocluster and the gold nanorod, and the FRET efficiency between the testosterone and the gold nanocluster is improved by regulating and controlling synthesis conditions and performing functional modification, so that the specificity and high-efficiency detection of testosterone molecules are realized.

In order to solve the technical problems, the invention is realized by the following technical scheme: the invention provides a method for detecting testosterone by a gold nanocluster-gold nanorod novel immunosensor, which comprises the following steps: step 1, preparing gold nanoclusters (fluorescence donors) and gold nanorods (fluorescence acceptors); step 2, respectively modifying and connecting the gold nanoclusters (fluorescence donors) and the gold nanorods (fluorescence acceptors); step 3, detecting testosterone molecules, wherein the gold nanoclusters (fluorescence donors) are in modification connection with testosterone, and the gold nanorods (fluorescence acceptors) are in modification connection with testosterone antibodies; the step 3 is as follows:

1) mixing, namely mixing gold nanocluster-testosterone solution with the same volume with testosterone antibody-gold nanorod solution for reaction to prepare mixed solution A;

2) testing the fluorescence property of the mixed solution A, detecting the change of the fluorescence property under different conditions, selecting the corresponding detection condition when the absolute value of the change of the fluorescence emission intensity is maximum, and determining the detection condition as the optimal condition;

3) and (3) detecting, namely mixing the mixed solution A with a testosterone molecular solution (to-be-detected solution), detecting under the optimal condition in the step 2), and obtaining the concentration of testosterone according to the change of fluorescence emission intensity and ultraviolet visible absorption intensity.

Further, the preparation method of the gold nanocluster (fluorescence donor) comprises the following steps:

step A1: adding HAuCl₄Diluting with 10.0mM water, adding Glutathione (GSH) (10.0mM), and adding HAuCl into the above components₄The volume ratio of water to glutathione is 1 (1-2) to 0.5-1, and the reaction is carried out for 30-70 minutes under the stirring of 1200rpm at the temperature of 90 ℃ to obtain a reaction solution a 1;

step A2: centrifuging the reaction solution a1 at 11000rpm for 20-40 min to obtain supernatant a 2;

step A3: dialyzing the supernatant a2 with a dialysis membrane for 40-60 hours to remove unreacted organic matters to obtain gold nanocluster liquid a 3;

step A4: and (3) freeze-drying the gold nanocluster liquid a3 to obtain the gold nanoclusters (fluorescence donors).

Further, the preparation method of the gold nanorod (fluorescence acceptor) comprises the following steps:

step B1: ice cold sodium oleate solution is added to HAuCl prepared in CTAB solution under stirring₄In solution, the solution turned yellow to brown, indicating the formation of gold species b 1;

step B2: gold seed b1 was aged for 1-3 hours to allow unreacted NaBH₄Hydrolyzing, and amplifying the growth process in proportion to obtain gold nanorods;

step B3: mixing 0.1mM CTAB solution, 4mM silver nitrate solution, 25mM HAuCl₄The aqueous solution and the 1mM HCl solution were sequentially added to mix, and 0.0788mM ascorbic acid was then added thereto asReducing agent, and homogenizing the mixture by stirring to obtain a mixture b 3; CTAB solution, silver nitrate solution, and HAuCl₄The volume ratio of the aqueous solution, the HCl solution and the ascorbic acid is 100: (5-10): (1-2): 0.6-2): 0.5-1.2);

step B4: adding 0.5-1.5% of gold seeds in volume ratio into the mixture b3, and standing the whole solution for 36 hours to obtain gold nanorods.

Further, the gold nanoclusters (fluorescence donors) are linked to testosterone modifications as follows:

step C1: adding the gold nanocluster solution into a mixed solution containing PBS (0.01mol/L, pH 7.4) and 4mg/mL EDC to prepare a mixed solution c1, and stirring the mixed solution c1 for 5 minutes and then for 20 minutes to activate free carboxylic acid groups on the gold nanoclusters;

step C2: adding 0.15mg/mL sulfo-NHS to the mixed solution c1 to prepare a mixed solution c2, and stirring the mixed solution c2 for 20 minutes;

step C3: adding 0.01mg/mL testosterone solution into the mixed solution c2 to obtain a mixed solution c3, reacting at 37 ℃ in the dark for 2 hours, and storing the mixed solution c3 at 4 ℃; the volume ratio of the gold nanocluster solution, the PBS, the EDC, the sulfo-NHS and the testosterone solution is 1: 3:(0.8-1.2): (0.04-0.08): (0.01-0.02);

step C4: performing tests through a fluorescence spectrum and an ultraviolet visible absorption spectrum to obtain the spectrum overlapping of the gold nanocluster-testosterone and testosterone antibody-gold nanorod system; modifying and connecting the gold nanoclusters (fluorescence donors) and testosterone antibodies to obtain a gold nanocluster-testosterone solution;

the gold nanorods (fluorescent receptors) are modified and connected with testosterone antibodies as follows:

step D1: adding 10 mug/mL testosterone antibody to 47.8 mug/mL gold nanorods under magnetic stirring; after stirring for 10 minutes, 3% PEG20000 is added as a stabilizer to obtain a mixture d 1; the volume ratio of the testosterone antibody, the gold nanorods and the PEG20000 of each component is as follows: 1:(50-85): (0.13-0.4);

step D2: and stirring the mixture d1 for 15 minutes and storing at 4 ℃ to realize the modified connection of the gold nanorods and the testosterone antibody, so as to obtain a gold nanorod and testosterone antibody solution.

Therefore, the invention has the following beneficial effects:

the invention relates to a method for detecting testosterone by a gold nanocluster-gold nanorod novel immunosensor, which selects gold nanoclusters with biocompatibility, good optical performance and easiness in synthesis as fluorescence donors, selects gold nanorods with adjustable LSPR peaks as fluorescence acceptors, connects testosterone and testosterone antibodies with the gold nanoclusters and the gold nanorods respectively, improves FRET efficiency between the testosterone antibodies and the gold nanoclusters by regulating synthesis conditions and performing functional modification, and realizes specificity and high-efficiency detection of testosterone molecules:

1. by means of the current methods for preparing and characterizing the gold nanoclusters and the gold nanorods, the emission spectrum of the gold nanoclusters and the absorption spectrum of the gold nanorods are maximally overlapped by regulating and controlling synthesis conditions and selecting the type of a protective ligand, and the FRET performance of the immunosensor is improved.

2. The surfaces of the gold nanoclusters and the gold nanorods are functionally modified to be respectively and effectively connected with testosterone and testosterone antibodies, the distance between a donor and an acceptor is shortened by utilizing the principle of combining the antibodies and antigen specificity, and the FRET efficiency is further enhanced.

3. The testosterone molecule is subjected to sensing detection application, and the sensitive, rapid and specific detection of the testosterone molecule is realized by exploring the optimal detection condition.

The foregoing description is only an overview of the technical solutions of the present invention, and in order to make the technical means of the present invention more clearly understood, the present invention may be implemented in accordance with the content of the description, and in order to make the above and other objects, features, and advantages of the present invention more clearly understood, the following detailed description is given in conjunction with the preferred embodiments, together with the accompanying drawings.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings of the embodiments will be briefly described below.

FIG. 1 is a reaction scheme of the present invention.

Detailed Description

Other aspects, features and advantages of the present invention will become apparent from the following detailed description, taken in conjunction with the accompanying drawings, which form a part of this specification, and which illustrate, by way of example, the principles of the invention. In the referenced drawings, the same or similar components in different drawings are denoted by the same reference numerals.

Referring to fig. 1, in the first embodiment:

a method for detecting testosterone by a gold nanocluster-gold nanorod novel immunosensor comprises the following steps: step 1, preparing gold nanoclusters (fluorescence donors) and gold nanorods (fluorescence acceptors); step 2, respectively modifying and connecting the gold nanoclusters (fluorescence donors) and the gold nanorods (fluorescence acceptors); step 3, detecting testosterone molecules, wherein the gold nanoclusters (fluorescence donors) are in modification connection with testosterone, and the gold nanorods (fluorescence acceptors) are in modification connection with testosterone antibodies; the step 3 is as follows:

1) mixing, namely mixing gold nanocluster-testosterone solution and testosterone antibody-gold nanorod solution with the same volume under the conditions of normal temperature and pH value of 5.5 to react to prepare mixed solution A;

2) testing the fluorescence property of the mixed solution A, detecting the change of the fluorescence property under different conditions, selecting the corresponding detection condition when the absolute value of the change of the fluorescence emission intensity is maximum, and determining the detection condition as the optimal condition;

3) and (3) detecting, namely mixing the mixed solution A with a testosterone molecular solution (to-be-detected solution), detecting under the optimal condition in the step 2), and obtaining the concentration of testosterone according to the change of fluorescence emission intensity and ultraviolet visible absorption intensity.

Specifically, the preparation method of the gold nanocluster (fluorescence donor) comprises the following steps:

step A1: adding HAuCl₄Diluting with 10.0mM water, adding Glutathione (GSH) (10.0mM), and adding HAuCl into the above components₄Reacting water and glutathione at the volume ratio of 1:1:0.5 for 30 minutes under the stirring of 1200rpm at the temperature of 90 ℃ to obtain a reaction solution a 1;

step A2: centrifuging the reaction solution a1 at 11000rpm for 20-minutes to obtain supernatant a 2;

step A3: dialyzing the supernatant a2 by a dialysis membrane for 40 hours to remove unreacted organic matters to obtain gold nanocluster liquid a 3;

step A4: the gold nanocluster liquid a3 was freeze-dried to obtain gold nanoclusters (fluorescence donors) that fluoresce at 840 nm.

Specifically, the preparation method of the gold nanorod (fluorescence acceptor) comprises the following steps:

step B1: ice cold sodium oleate solution is added to HAuCl prepared in CTAB solution under stirring₄In solution, the solution turned yellow to brown, indicating the formation of gold species b 1;

step B2: gold seed b1 was aged for 1 hour to allow unreacted NaBH₄Hydrolyzing, and amplifying the growth process in proportion to obtain gold nanorods;

step B3: mixing 0.1mM CTAB solution, 4mM silver nitrate solution, 25mM HAuCl₄The aqueous solution and 1mM HCl solution were added in this order to mix, and then 0.0788mM ascorbic acid was added thereto as a reducing agent, and the mixture was homogenized by stirring to give a mixture b 3; CTAB solution, silver nitrate solution, and HAuCl₄The volume ratio of the aqueous solution, the HCl solution and the ascorbic acid is 100: 5:1:0.6: 0.5;

step B4: 0.5% by volume of gold seed was added to the above mixture b3, and the whole solution was allowed to stand for 36 hours to obtain gold nanorods at 842 nm.

Specifically, the gold nanoclusters (fluorescence donors) and testosterone modification connection are as follows:

step C1: adding the gold nanocluster solution into a mixed solution containing PBS (0.01mol/L, pH 7.4) and 4mg/mLEDC to prepare a mixed solution c1, stirring the mixed solution c1 for 5 minutes, and then stirring for 20 minutes to activate free carboxylic acid groups on the gold nanoclusters;

step C2: adding 0.15mg/mL sulfo-NHS to the mixed solution c1 to prepare a mixed solution c2, and stirring the mixed solution c2 for 20 minutes;

step C3: adding 0.01mg/mL testosterone solution into the mixed solution c2 to prepare a mixed solution c3, reacting for 2 hours at the temperature of below 37 ℃ in the dark, and storing the mixed solution c3 at the temperature of 4 ℃, wherein the volume ratio of the gold nanocluster solution, the PBS, the EDC, the sulfo-NHS and the testosterone solution is 1:3:0.8:0.04: 0.01;

step C4: performing tests through a fluorescence spectrum and an ultraviolet visible absorption spectrum to obtain the spectrum overlapping of the gold nanocluster-testosterone and testosterone antibody-gold nanorod system; modifying and connecting the gold nanoclusters (fluorescence donors) and testosterone antibodies to obtain a gold nanocluster-testosterone solution;

the gold nanorods and the testosterone antibody are modified and connected as follows:

step D1: adding 10 mug/mL testosterone antibody to 47.8 mug/mL gold nanorods under magnetic stirring; after stirring for 10 minutes, 3% PEG20000 was added as stabilizer mixture d 1; the volume ratio of the testosterone antibody, the gold nanorods and the PEG20000 of each component is as follows: 1:50: 0.13;

step D2: and stirring the mixture d1 for 15 minutes and storing at 4 ℃ to realize the modified connection of the gold nanorods and the testosterone antibody, so as to obtain a gold nanorod and testosterone antibody solution.