阅读说明：本技术 一种获取评估消化道损伤模型腹胀指数的方法 (Method for obtaining abdominal distension index of digestive tract injury assessment model ) 是由 蒋雪薇 魏鹏举 金朋 邓水香 宫晔 田觅 于 2019-09-23 设计创作，主要内容包括：本发明涉及生物技术领域,尤其涉及一种获取评估消化道损伤模型腹胀指数的方法,包括如下步骤：对实验体进行预训练；采集经预训练后的实验体在实验所需时间节点的腹正面图像,并将其存储；调取腹正面图像,提取第一数据和第二数据；计算第一数据与第二数据之比值,并将其作为腹胀指数；应用腹胀指数评估实验体的消化道肿胀程度。本发明通过对同一动物不同时间节点进行连续多次评估,提高了评估结果的准确度,且方便快捷,高效无创,降低成本,减少设备依赖性,间接量化消化道肿胀程度进而便于对消化道损伤模型的消化道进行评估。(The invention relates to the technical field of biology, in particular to a method for obtaining and evaluating an abdominal distension index of a digestive tract injury model, which comprises the following steps: pre-training an experimental body; acquiring an abdominal front image of the pre-trained experimental body at a time node required by the experiment, and storing the abdominal front image; calling a front image of the abdomen, and extracting first data and second data; calculating the ratio of the first data to the second data, and taking the ratio as an abdominal distension index; the degree of gut swelling in the subjects was assessed using the abdominal distension index. According to the invention, the nodes of the same animal at different times are evaluated continuously and repeatedly, so that the accuracy of the evaluation result is improved, the method is convenient, rapid, efficient and noninvasive, the cost is reduced, the equipment dependence is reduced, and the swelling degree of the digestive tract is quantified indirectly, thereby facilitating the evaluation of the digestive tract injury model.)

1. A method for obtaining an abdominal distension index of an assessment digestive tract injury model is characterized by comprising the following steps:

pre-training an experimental body;

acquiring an abdominal front image of the pre-trained experimental body at a time node required by the experiment, and storing the abdominal front image;

calling the abdomen front image, and extracting first data and second data in the abdomen front image;

and calculating the ratio of the first data to the second data, and taking the ratio as an abdominal distension index.

2. The method for obtaining the abdominal distension index of the digestive tract injury model for evaluation according to claim 1, wherein the pre-training comprises the following specific steps:

continuously training the experimental body for 3 days before the digestive tract injury model is established; the tail part is lifted to invert the experimental body, so that the head part of the experimental body is downward and the body is naturally stretched;

training is carried out 3-5 times per day, and the training has no physical contracture and muscle tension stress reaction when the training is inverted.

3. The method for obtaining the abdominal distension index of the digestive tract injury model according to claim 1, wherein the specific step of acquiring the abdominal frontal image of the pre-trained experimental body at the time node required by the experiment comprises:

setting a body position: lifting the pre-trained experimental body by the tail part according to the time node required by the experiment, enabling the head part of the experimental body to stretch downwards to the body naturally, and grasping forelimbs on a pre-fixed metal grid;

acquiring an image: shooting the abdomen front of the pre-trained experimental body, and storing the abdomen front images obtained by shooting and corresponding time nodes in a one-to-one correspondence mode.

4. The method for obtaining the abdominal distension index of the digestive tract injury model according to claim 3, wherein the metal mesh is formed by splicing a plurality of metal strips and is fixed on a test bench table, and the metal mesh is at least 15cm higher than the test bench table.

5. The method for obtaining the abdominal distension index of the digestive tract injury model according to claim 3, wherein a 12MP wide-angle lens is used and is spaced 30-40 cm away from the experimental body so as to take a picture of the abdominal front.

6. The method for obtaining the abdominal distension index of the digestive tract injury model according to claim 1, wherein the specific steps of retrieving the abdominal frontal image and extracting the first data and the second data include:

and (3) image calling: using image J software to call a pre-trained experiment abdomen front image corresponding to the experiment time node;

extracting data in the image: measuring the image by using a straight line measuring tool in image J software, and taking the width of the widest part of the abdomen of the pre-trained experimental body as first data, namely L1; and taking a connecting line which is perpendicular to the body axis and from the intersection point of the skin on the outer side of the inguinal and femoral head of the pre-trained experimental body to the outer side of the homonymous humerus root as second data, recording the second data as L2, and storing and exporting the result.

7. The method for obtaining the abdominal distension index of the digestive tract injury model according to claim 1, wherein the step of calculating the ratio of the first data to the second data as the abdominal distension index comprises:

extracting the first data and the second data through Excel software;

and calculating the ratio of the second data to the first data, and recording the ratio as an abdominal distension index.

8. The method for obtaining the abdominal distension index of the digestive tract injury model according to claim 1, wherein the abdominal distension index is used to evaluate the degree of digestive tract swelling of the pre-trained subjects after obtaining the abdominal distension index.

Technical Field

The invention relates to the technical field of biology, in particular to a method for obtaining and evaluating an abdominal distension index of a digestive tract injury model.

Background

The damage of the digestive tract can cause dysfunction of the digestive tract and influence physiological activities of nutrition absorption, immunity and the like of patients. In daily life, the damage of the digestive tract is caused by various factors, such as the stimulation of toxic substances, intestinal infection, mechanical stimulation and other factors to the digestive tract, and secondary digestive tract damage can be caused by trauma, burn, stroke and other organ injuries. With the progress of research on various diseases related to intestinal injury, various rat intestinal injury models are widely applied. The manifestations of gastrointestinal mucosa injury, permeability enhancement, intestinal peristalsis reduction and gastrointestinal swelling are observed in a rat intestinal injury model, and the manifestations are related to survival conditions.

The swelling of the digestive tract is one of important characteristics reflecting the injury of the digestive tract, and the existing assessment method for the injury of the digestive tract is classified into invasive type and non-invasive type according to the degree of trauma. The common invasive methods generally include carbon powder propelling method, laparotomy direct observation method, histological damage assessment and the like, and the common noninvasive methods include disease activity index assessment, imaging assessment and the like.

The invasive methods are all to observe the change of body tissues at the death time point and the death time point of a dead experimental animal, the operation process is complex, the change of the body tissues at different time points can not be observed on the same animal, and the animal cost is higher; the non-invasive method is expensive in equipment, high in professional technical requirement, mainly depends on subjective judgment of technicians, and is not convenient to be widely used.

Disclosure of Invention

In view of the problems of complex operation, high cost or high equipment dependence of the conventional common method for evaluating the alimentary canal swelling, the invention provides the method for obtaining the abdominal distension index of the alimentary canal injury model, which can be used for continuously evaluating the same animal for multiple times at different time points, improves the accuracy of an evaluation result, and is efficient, noninvasive, low in cost and low in equipment dependence.

In order to achieve the purpose, the implementation case of the invention adopts the following technical scheme:

the invention provides a method for obtaining an abdominal distension index of an assessment digestive tract injury model, which comprises the following steps: pre-training an experimental body; acquiring an abdominal front image of the pre-trained experimental body at a time node required by the experiment, and storing the abdominal front image; calling a belly front image, and extracting first data and second data in the belly front image; and calculating the ratio of the first data to the second data, and taking the ratio as the abdominal distension index.

According to one aspect of the invention, the pre-training comprises the following specific steps: carrying out adaptability training on the experimental body: continuously carrying out 3 days before establishing a digestive tract injury model, lifting the tail of the experimental body, inverting the tail of the experimental body to enable the head of the experimental body to be downward, and naturally stretching the body; training 3-5 times per day to avoid physical contracture and muscular stress reaction when the patient is inverted; autologous blood was injected into the basal ganglia to construct a test body with gastrointestinal lesions caused by cerebral hemorrhage.

According to one aspect of the invention, the specific step of acquiring the abdominal front image of the pre-trained experimental body at the time node required by the experiment comprises the following steps: setting a body position: inverting the pre-trained experimental body by the lifting tail part according to the time node required by the experiment, enabling the head part of the experimental body to stretch downwards and naturally, and grabbing forelimbs on a pre-fixed metal grid; acquiring an image: shooting the abdomen front of the pre-trained experimental body, and storing the abdomen front images obtained by shooting and corresponding time nodes in a one-to-one correspondence mode.

According to one aspect of the invention, according to the time nodes required by the experiment, namely 2 hours, 3 days and 7 days after the pre-trained experimental body is generated, the tail part is lifted upside down to invert the pre-trained experimental body, the head part is downward, the body is naturally stretched, and the forelimbs are held on the pre-fixed metal grid.

According to one aspect of the invention, the metal grid is formed by splicing a plurality of metal strips and is fixed on the table top of the test bed, and the metal grid is at least 15cm higher than the table top of the test bed.

According to one aspect of the present invention, a 12MP wide angle lens is used at a distance of 30 to 40cm from the subject to photograph the ventral front.

According to one aspect of the invention, the specific steps of retrieving the image and extracting the first data and the second data comprise: calling an image corresponding to a time node required by an experiment; the specific steps of measuring the image to obtain the first data and the second data include: and (3) image calling: using image J software to call a pre-trained experiment abdomen front image corresponding to the experiment time node; extracting data in the image: measuring the image by using a straight line measuring tool in image J software, and taking the width of the widest part of the abdomen of the pre-trained experimental body as first data, and recording as L1; and (3) taking a connecting line which is perpendicular to the body axis and from the intersection point of the skin on the outer side of the inguinal and femoral head of the pre-trained experimental body to the outer side of the homonymous humerus root as second data, recording the second data as L2, and storing and exporting the result.

According to one aspect of the present invention, the specific step of calculating the ratio of the first data to the second data as the abdominal distension index comprises: extracting first data and second data through Excel software; the first data is compared to the second data and the ratio is recorded as the abdominal distension index.

In accordance with one aspect of the invention, after obtaining the bloating index, the bloating index is used to assess the degree of gut swelling in pre-trained subjects.

The implementation of the invention has the advantages that: the experimental body is adaptively exercised to be adaptive to the reverse lifting state, so that the experimental body in the reverse lifting state is conveniently photographed at the time point required by the experiment, the photographed picture is measured and calculated, the continuous and multiple evaluations are carried out on the same animal at different time points, the accuracy of the evaluation result is improved, the method is convenient and rapid, efficient and noninvasive, the cost is reduced, the equipment dependence is reduced, and the gastrointestinal swelling degree is indirectly quantified so as to conveniently evaluate the gastrointestinal tract of the gastrointestinal injury model.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without creative efforts.

FIG. 1 is a flow chart of a method for evaluating the degree of swelling of the digestive tract of a model of digestive tract injury according to the present invention;

FIG. 2 is a schematic diagram of a front measurement of the abdomen of an experimental rat according to the present invention;

FIG. 3 is a graph comparing the weight of the digestive tract in the non-swollen group and the swollen group according to the present invention;

FIG. 4 is a graph comparing the abdominal distension index of the no swelling group and the swollen group according to the present invention;

FIG. 5 is a graph of the relationship between the weight of the digestive tract and the abdominal distension index according to the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In order to solve the technical problem that the operation is complex and an accurate evaluation result of the swelling degree of the digestive tract is not easy to obtain, the invention provides the method for obtaining the abdominal distension index of the digestive tract injury evaluation model, which can be used for continuously evaluating the same animal for multiple times at different time points, improves the accuracy of the evaluation result, and is efficient, noninvasive and low in cost. The method for obtaining the abdominal distension index of the digestive tract injury model will be described in detail with reference to fig. 1 to 4.

As shown in fig. 1, fig. 1 is a flowchart of a method for obtaining an abdominal distension index of an assessment digestive tract injury model provided by the present invention, where the method for obtaining an abdominal distension index of an assessment digestive tract injury model includes the following steps:

s1, pre-training the subject.

Since there are many diseases causing the gastrointestinal injury, in order to avoid repeated explanation, the present application may select an experimental rat as an experimental subject, and select the gastrointestinal injury caused by cerebral hemorrhage as an example for research, during the actual research process, other animals may be selected as experimental subjects according to the experimental purpose, experimental conditions, etc., or the gastrointestinal injury caused by other diseases may be selected for research, which is not further limited in the present application. In this embodiment, the experimental rat needs to be pre-trained, and the specific steps include: the experimental rats were subjected to adaptive training. Specifically, the experimental body is inverted by lifting the tail part of the experimental body 3 days before the digestive tract injury model is established, so that the head part of the experimental body is downward and the body is naturally stretched; training 3-5 times per day to avoid stress reaction such as body curl and muscle tension when the patient is inverted; and constructing a digestive tract injury model. In this embodiment, the specimen with the damaged digestive tract caused by cerebral hemorrhage can be constructed by injecting autologous blood to the basal ganglia, so as to construct a digestive tract damage model.

In the actual training process, the time that different experimental bodies adapt to the inversion training is different, and the training days of different experimental bodies are different promptly, and the setting of training days is according to the experimental body setting of actually choosing for use, does not further prescribe this in this application.

In the present application, the specific steps of injecting autologous blood into the basal ganglia to construct a model of digestive tract injury caused by cerebral hemorrhage include: the specific injection location parameters are 1.5mm from the anterior bregma of the experimental rat, 3.5mm from the lateral side of the right side, 6.0mm deep, and the injection blood volume is 100 mul.

And S2, acquiring the abdominal front image of the pre-trained experimental body at the time node required by the experiment, and storing the abdominal front image.

In a specific implementation manner, the specific step of acquiring the abdomen front image of the pre-trained experimental rat at the time node required by the experiment comprises: setting a body position: lifting the pre-trained experimental rat by the tail part according to the time node required by the experiment, enabling the head part of the pre-trained experimental rat to stretch downwards to the body naturally, and grabbing forelimbs on a pre-fixed metal grid; acquiring an image: shooting the front of the abdomen of the pre-trained experimental rat, and storing the front image of the abdomen obtained by shooting and the corresponding time node in a one-to-one correspondence manner. In the present application, the number of back-lifts is set according to whether the pre-trained experimental rat is in a relaxed state, and generally the number of back-lifts is set to be one or two.

In this embodiment, the time nodes required for the experiment are two hours, three days and seven days after the pre-trained experimental rat digestive tract injury model is generated, and further, 2 hours, 3 days and 7 days after the pre-trained experimental rat is generated, the pre-trained experimental rat is inverted by lifting the tail, the head is downward, the body is naturally stretched, and the forelimb is held on the pre-fixed metal grid. In the actual experiment process, in order to improve the accuracy of the evaluation result, one or more time nodes can be provided, and the time nodes are selected according to the experiment requirements and the specifically selected experimental body.

In this embodiment, the metal grid is formed by splicing a plurality of metal strips and is fixed on the test table, in order to facilitate the pre-trained forelimb of the experimental rat to grab the metal grid, the metal grid needs to be at least 15cm higher than the test table, in addition, the diameter of the metal strips is 1mm, and the size of the metal grid is 1cm x 8 cm.

In order to collect a clearer image, the image in the application adopts a 12MP wide-angle lens to shoot the pre-trained experimental rat, a focal length of 27mm and 1200 ten thousand pixels are selected, the distance between the wide-angle lens and the abdominal front of the pre-trained experimental rat is 30-40 cm, and the shot image needs to take the abdominal front of the pre-trained experimental rat as the center and be parallel to the abdominal front of the experimental rat.

S3, the abdomen front image is retrieved, and the first data and the second data in the abdomen front image are extracted.

Specifically, a pre-trained abdominal front image of an experimental rat corresponding to an experimental time node is called through image J software; measuring the image by using a linear measuring tool in image J software, and taking the width of the widest part of the abdomen of the pre-trained experimental rat obtained by measurement as first data, wherein the first data is marked as L1 and the unit is pixel; a line from the intersection point of the skin on the outer side of the femoral head and the groin of the pre-trained experimental rat to the outer side of the humerus root on the same side, which is perpendicular to the body axis, is used as second data, which is recorded as L2 and has a unit of pixel, as shown in fig. 2.

And S4, calculating the ratio of the first data to the second data, and storing the ratio as the abdominal distension index.

After the first data and the second data are obtained through image J software measurement, the first data and the second data are extracted through the ecxel software, and the ratio of the first data to the second data is calculated and recorded as an abdominal distension index.

Specifically, the first data L1 is divided by the second data L2, and the obtained value is referred to as Abdominal Distension Index (ADI), and the calculation formula is L1/L2.

In the application, after the abdominal distension index is obtained, the abdominal distension index can be used for evaluating the digestive tract swelling degree of the experimental rat, and further, the Abdominal Distension Index (ADI) is used in combination with other experimental indexes, such as digestive tract pathology and the like, for evaluating the digestive tract swelling degree of the experimental rat; or continuous evaluation of the digestive tract swelling of experimental rats based on the Abdominal Distension Index (ADI) measured at different time nodes required for the experiment.

And after the corresponding abdominal distension index is obtained for any time node, evaluating the swelling degree of the digestive tract according to the multiple abdominal distension indexes. In this example, the experimental rat was subjected to laparotomy to observe the actual swelling degree of the digestive tract thereof, and was weighed by taking the gastrointestinal tract from the cardia to the anus thereof, and then the model was divided into a digestive tract non-swelling group and a swelling group according to the laparotomy observation results, and the non-swelling group was compared with the swelling group.

The experiment shows that the weight of the digestive tract of the swollen group is increased extremely remarkably (P)<0.01), as shown in fig. 3, the abdominal distension index calculated by the present invention is significantly increased, as shown in fig. 4, and the weight of the digestive tract has a significant positive correlation (P) with the Abdominal Distension Index (ADI)<0.05,R²0.4602) as shown in fig. 5, thereby indicating that the present invention is capable of non-invasively assessing the degree of gut swelling.

In summary, the experimental rat is adaptively exercised to adapt to the reverse lifting state, so that the experimental rat in the reverse lifting state can be conveniently photographed at the time point required by the experiment, the photographed picture is measured and calculated, and the same animal can be continuously evaluated for multiple times at different time points, so that the accuracy of the evaluation result is improved, and the method is convenient, rapid, efficient and noninvasive, reduces the cost, reduces the equipment dependence, indirectly quantifies the swelling degree of the digestive tract, and further facilitates the evaluation of the digestive tract injury model.

The above description is only an embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention disclosed herein are intended to be covered by the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.