Pesticide effect experimental device and method for indoor space insect repellents

文档序号:1612447 发布日期:2020-01-10 浏览:19次 中文

阅读说明:本技术 一种室内空间驱虫剂药效实验装置及实验方法 (Pesticide effect experimental device and method for indoor space insect repellents ) 是由 陈斌 罗德跃 闫振天 于 2019-10-21 设计创作,主要内容包括:本发明公开了一种室内空间驱虫剂药效实验装置及实验方法,该实验装置广泛用于空间型驱避剂,其结构简单,操作便捷。该实验装置由三个有机玻璃方箱,方箱由两个带活门页的有机玻璃管链接以及两个排风装置和两个吸风装置,另外中间方箱正面还设有一个放蚊口,本发明实验装置操作简单,结果可靠,重复性强,能满足空间驱避类药物药效测试的要求。(The invention discloses an indoor space insect repellant pesticide effect experimental device and an indoor space insect repellant pesticide effect experimental method. The experimental device is composed of three organic glass square boxes, wherein each square box is formed by connecting two organic glass tubes with valve leaves, two air exhaust devices and two air suction devices, and the front surface of the middle square box is also provided with a mosquito placing port.)

1. The utility model provides an indoor space expelling parasite agent drug effect experimental apparatus which characterized in that: including control box, mosquito case and proof box, the control box the mosquito case with the proof box, one side of control box with communicate with each other through the pipe between one side of mosquito case and be connected, the opposite side of mosquito case with communicate with each other through the pipe between one side of proof box and be connected, the front end of mosquito case be provided with inboard communicating mosquito bag, the control box with be provided with the fan system that is used for the circulation of air on the proof box.

2. The indoor space insect repellent pesticide effect experimental facility of claim 1, characterized in that: the fan system comprises a first suction fan, a second suction fan, a first exhaust fan and a second exhaust fan, the first suction fan is arranged at the upper end of the comparison box, the second suction fan is arranged at the upper end of the test box, the first discharge machine is arranged at the lower end of the comparison box, and the second discharge machine is arranged at the lower end of the test box.

3. The indoor space insect repellent pesticide effect experimental apparatus of claim 2, wherein: the motor models of the first suction fan, the second suction fan, the first exhaust fan and the second exhaust fan are AP-3000, the power is 16w, and the maximum air volume is 50L/min.

4. An experiment method for pesticide effect of an indoor space insect repellant is characterized by comprising the following steps:

(one) blank test

50 female mosquitoes which do not suck blood are placed in the mosquito box, the mosquito placing opening is closed, the valve leaf on the connecting pipe is opened, and the number of the mosquitoes in the control box and the test box is observed;

(II) repellency test

(1) A proper amount of qualified mosquitoes are put into the mosquito box;

(2) putting a reference substance and an object to be detected, and simultaneously opening an air suction and exhaust system;

(3) observing the number of the test insects in the two boxes, closing a valve page on the connecting pipe after 20min, recording data, and clearly confirming the experiment box after each experiment;

(4) each substance to be tested is repeated for 3 times, so that the repeatability of the experiment is ensured;

(5) the positions of the experiment box and the comparison box are exchanged to ensure the randomness of the experiment;

(III) calculation of the repellency Rate

According to the following formula:

Figure FDA0002240842290000021

Technical Field

The invention relates to the technical field of space mosquito repellent testing devices, in particular to an indoor space insect repellent drug effect experimental device and an indoor space insect repellent drug effect experimental method.

Background

Along with the improvement of the living standard of people, more and more mosquito repellent products are on the market, such as mosquito repellent pastes, mosquito repellent liquid, mosquito repellent bracelet etc. but do not have a standard drug effect experimental apparatus to this kind of spatial type repellent, are urgent needs at present. Among them, mosquitoes are a very important sanitary pest, which suck blood by stinging, thus disturbing people and spreading various infectious diseases. Various diseases such as dengue fever, malaria, filariasis, etc. can be transmitted by blood-sucking. At present, mosquito-proof repellents at home and abroad are researched a lot, repellent products are more and more, except that a liquid smearing type repellent needs to be used for an experiment by a human body, other repellent products also have a lot of devices for detecting repellent effects, and a set of standard method and a set of unified experimental device do not exist, so that an improvement space exists.

Disclosure of Invention

The invention aims to solve the problems and provide an indoor space insect repellant drug effect experimental device and an indoor space insect repellant drug effect experimental method.

The invention realizes the purpose through the following technical scheme:

the invention relates to an indoor space insect repellant pesticide effect experimental device, which is characterized in that: including control box, mosquito case and proof box, the control box the mosquito case with the proof box, one side of control box with communicate with each other through the pipe between one side of mosquito case and be connected, the opposite side of mosquito case with communicate with each other through the pipe between one side of proof box and be connected, the front end of mosquito case be provided with inboard communicating mosquito bag, the control box with be provided with the fan system that is used for the circulation of air on the proof box.

Further, the fan system comprises a first suction fan, a second suction fan, a first exhaust fan and a second exhaust fan, the first suction fan is arranged at the upper end of the comparison box, the second suction fan is arranged at the upper end of the test box, the first exhaust machine is arranged at the lower end of the comparison box, and the second exhaust machine is arranged at the lower end of the test box.

Preferably, the motor models of the first suction fan, the second suction fan, the first exhaust fan and the second exhaust fan are AP-3000, the power is 16w, and the maximum air volume is 50L/min.

The invention discloses a method for testing the pesticide effect of an indoor space insect repellant, which is characterized by comprising the following steps of:

(one) blank test

50 female mosquitoes which do not suck blood are placed in the mosquito box, the mosquito placing opening is closed, the valve leaf on the connecting pipe is opened, and the number of the mosquitoes in the control box and the test box is observed;

(II) repellency test

(1) A proper amount of qualified mosquitoes are put into the mosquito box;

(2) putting a reference substance and an object to be detected, and simultaneously opening an air suction and exhaust system;

(3) observing the number of the test insects in the two boxes, closing a valve page on the connecting pipe after 20min, recording data, and clearly confirming the experiment box after each experiment;

(4) each substance to be tested is repeated for 3 times, so that the repeatability of the experiment is ensured;

(5) the positions of the experiment box and the comparison box are exchanged to ensure the randomness of the experiment;

(III) calculation of the repellency Rate

According to the following formula:

Figure BDA0002240842300000021

in the formula: NC represents the number of mosquitoes in the control group, NT represents the number of mosquitoes in the experimental group, and N represents the number of mosquitoes to be detected placed in the mosquito box.

The invention has the beneficial effects that:

the invention relates to an indoor space insect repellant drug effect experimental device and an indoor space insect repellant drug effect experimental method, and compared with the prior art, the indoor space insect repellant drug effect experimental device has the following beneficial effects:

1. the experimental device designed by the invention utilizes the air suction device and the air exhaust device to enable the air in the control box and the air in the test box to be in an independent circulation state in the glass boxes of the control and test groups, thereby avoiding the mutual influence between the object to be tested and the control object.

2. The device disclosed by the invention can be used for evaluating the repellent effect and carrying out a fumigation test, and when the movable door leaf of the connecting pipe is closed, the mosquito box becomes a fumigation test device, so that the dual purposes of one device and one device are achieved.

Drawings

Fig. 1 is a schematic structural view of the present invention.

In the figure: 1-a comparison box, 2-a mosquito box, 3-a test box, 4-a first suction fan, 5-a second suction fan, 6-a first exhaust fan, 7-a second exhaust fan and 8-a mosquito bag.

Detailed Description

The invention will be further described with reference to the accompanying drawings in which:

as shown in fig. 1: aiming at the defects of the prior device and the detection method and the test device of other mosquito repelling products without scientific standard on mosquito repelling effect, the experimental device is developed and provides a new reference for testing the drug effect of the mosquito repellent. The invention adopts the following technical scheme that the experimental device consists of three specially-made organic glass boxes, and the size of each box is 40cm multiplied by 40 cm. The top surface and the bottom surface of the comparison box and the test box are provided with holes with the diameter of 10cm, the holes on the top surface are used for installing air suction devices, the holes on the bottom surface are used for installing air exhaust devices, and the air suction devices and the air exhaust devices are installed to enable air in a laboratory to be in an independent flowing state so as to eliminate experiment interference; the front surface of the mosquito box is provided with a mosquito placing opening with the size of 20cm multiplied by 20 cm; the control box, the mosquito box and the test box are connected by a connecting pipe provided with a flap, and the length of the connecting pipe is 20 cm.

The invention relates to an indoor space insect repellant pesticide effect experimental device, which is characterized in that: including control box, mosquito case and proof box, the control box the mosquito case with the proof box, one side of control box with communicate with each other through the pipe between one side of mosquito case and be connected, the opposite side of mosquito case with communicate with each other through the pipe between one side of proof box and be connected, the front end of mosquito case be provided with inboard communicating mosquito bag, the control box with be provided with the fan system that is used for the circulation of air on the proof box.

Further, the fan system comprises a first suction fan, a second suction fan, a first exhaust fan and a second exhaust fan, the first suction fan is arranged at the upper end of the comparison box, the second suction fan is arranged at the upper end of the test box, the first exhaust machine is arranged at the lower end of the comparison box, and the second exhaust machine is arranged at the lower end of the test box.

Preferably, the motor models of the first suction fan, the second suction fan, the first exhaust fan and the second exhaust fan are AP-3000, the power is 16w, and the maximum air volume is 50L/min.

The invention discloses a method for testing the pesticide effect of an indoor space insect repellant, which is characterized by comprising the following steps of:

(one) blank test

50 female mosquitoes which do not suck blood are placed in the mosquito box, the mosquito placing opening is closed, the valve leaf on the connecting pipe is opened, and the number of the mosquitoes in the control box and the test box is observed;

(III) repelling experiments

(1) A proper amount of qualified mosquitoes are put into the mosquito box;

(2) putting a reference substance and an object to be detected, and simultaneously opening an air suction and exhaust system;

(3) observing the number of the test insects in the two boxes, closing a valve page on the connecting pipe after 20min, recording data, and clearly confirming the experiment box after each experiment;

(4) each substance to be tested is repeated for 3 times, so that the repeatability of the experiment is ensured;

(5) the positions of the experiment box and the comparison box are exchanged to ensure the randomness of the experiment;

(III) calculation of the repellency Rate

According to the following formula:in the formula: NC represents the number of mosquitoes in the control group, NT represents the number of mosquitoes in the experimental group, and N represents the number of mosquitoes to be detected placed in the mosquito box.

The principle of the invention is as follows: placing a certain amount of tested mosquitoes into a mosquito box and standing for 15 +/-5 min; adding a contrast agent and an insect repellent into a contrast box and an experimental box respectively, then opening an exhaust system and an air suction system simultaneously, opening a valve page of a connecting pipe, observing the number of mosquitoes to be tested in the contrast box and the experimental box after the experiment test is finished, and calculating the space repelling rate of the object to be tested to the mosquitoes according to a specific formula.

During the test, the tested mosquitoes are led into a No. ② glass box from a gauze screen at the temperature of 26 +/-2) DEG C and the relative humidity of 65 +/-10 percent, a shutter and the gauze screen are closed, then the mosquito box is covered by shading cloth and kept stand for 15min, a reference substance and an object to be tested are placed into a control box and a test box, an air suction device and an air exhaust device are opened simultaneously to enable air in the test box and the control box to flow through, the shutter on a connecting pipe is opened at the moment, the test is carried out for a certain time, finally, the number of the mosquitoes in the control box and the test box and the number of the knocked down mosquitoes are counted, and the mosquito repelling rate is calculated according to the number of the mosquitoes in the two boxes and the number of the knocked down mosquitoes.

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