阅读说明：本技术 一种红托竹荪生长促进剂及其制备方法和应用 (Dictyophora rubrovalvata growth promoter and preparation method and application thereof ) 是由 刘涛 陆荣生 湛年勇 唐璇 韩美丽 韦爱兰 陈进宁 邓莉杰 卢中强 李本丽 肖达 于 2019-10-15 设计创作，主要内容包括：本发明提供了一种红托竹荪生长促进剂及其制备方法和应用,属于食用菌栽培技术领域,所述生长促进剂,每升包括灵芝发酵液产物850～950mL,枸杞粉200～300g,氯吡脲1.6～2.0g；本发明将所述灵芝发酵液产物、枸杞粉和氯吡脲混合后,95～100℃加热25～35min获得所述生长促进剂。本发明提供的生长促进剂,能够促进红托竹荪菌丝生长,缩短红托竹荪的生长周期、提高产量。(The invention provides a Dictyophora rubrovalvata growth promoter and a preparation method and application thereof, and belongs to the technical field of edible fungus cultivation, wherein each liter of the growth promoter comprises 850-950 mL of lucid ganoderma fermentation liquid product, 200-300 g of medlar powder and 1.6-2.0 g of forchlorfenuron; the growth promoter is obtained by mixing the lucid ganoderma fermentation liquid product, the medlar powder and the forchlorfenuron and heating the mixture at the temperature of 95-100 ℃ for 25-35 min. The growth promoter provided by the invention can promote the growth of dictyophora rubrovolvata hyphae, shorten the growth period of the dictyophora rubrovolvata and improve the yield.)

1. A growth promoter for Dictyophora rubrovalvata is characterized by comprising 850-950 mL of lucid ganoderma fermentation liquid products, 200-300 g of Chinese wolfberry powder and 1.6-2.0 g of forchlorfenuron per liter.

2. The growth promoter according to claim 1, wherein the ganoderma lucidum fermentation broth product is prepared by the following method: inoculating the solid ganoderma lucidum mother strain into a primary fermentation culture medium, culturing at 25-28 ℃ for 4-6 days to obtain a primary liquid strain, and transferring the primary liquid strain to a secondary fermentation culture medium for culturing for 5-6 days to obtain a ganoderma lucidum fermentation liquid product.

3. The growth promoter according to claim 2, wherein the primary fermentation medium comprises the following components in 1000 g: 180-220 g of crushed folium cercis negundo, 180-220 g of dried Chinese yam slices, 30-40 g of milk powder, 20-30 g of pigeon pea powder, 2.0-3.0 g of yeast powder, 2.0-3.0 g of cellulose, 0.08-0.12 g of aspergillus oryzae bacterial powder, 0.08-0.12 g of bacillus bacterial powder, 1.5-2.5 g of monopotassium phosphate, 1.0-2.0 g of magnesium sulfate, 20-30 g of glucose and 0.5mL of tween-800.4;

the secondary fermentation medium comprises the following components in 1000 g: 340-360 g of crushed folium cercis negundo, 110-130 g of dried rhizoma dioscoreae slices, 10-20 g of milk powder, 10-20 g of pigeon pea powder, 2.0-3.0 g of yeast powder, 1.0-2.0 g of cellulose, 0.08-0.12 g of aspergillus oryzae bacterial powder, 0.08-0.12 g of bacillus bacterial powder, 1.5-2.5 g of monopotassium phosphate, 1.0-2.0 g of magnesium sulfate, 20-30 g of glucose and 0.3-0.5 mL of food antifoaming agent.

4. A process for producing a growth promoter according to any one of claims 1 to 3, comprising the steps of: and mixing the ganoderma lucidum fermentation liquid product produced by the secondary fermentation, medlar powder and forchlorfenuron, and heating at 95-100 ℃ for 25-35 min to obtain the growth promoter.

5. The Dictyophora rubrovalvata stock culture medium is characterized by comprising the following components in 1000 g: 350-450 g of cotton seed hulls, 150-250 g of cajan leaves, 200-300 g of cajan sawdust, 120-140 g of bagasse, 8-12 g of gypsum powder, 8-12 g of calcium superphosphate, 200-300 ml of the growth promoter according to any one of claims 1-3 and 300-450ml of water.

6. The Dictyophora rubrovalvata cultivar culture medium is characterized by comprising the following components in 1000 g: 350-450 g of cotton seed hulls, 100-200 g of cajan leaves, 200-260 g of cajan sawdust, 80-120 g of cassava dregs, 80-120 g of bagasse, 8-12 g of gypsum powder, 8-12 g of calcium superphosphate, 250ml of the growth promoter according to any one of claims 1-3 and 300-350ml of water.

7. The Dictyophora rubrovalvata fruiting bag culture medium is characterized by comprising the following components in 1000 g: 400-500 g of pigeon pea sawdust, 200-250 g of longan sawdust, 120-140 g of pigeon pea leaves, 80-120 g of cottonseed hulls, 70-90 g of bagasse, 8-12 g of gypsum powder, 8-12 g of calcium superphosphate, 200ml of the growth promoter according to any one of claims 1-3 and 400-350ml of water.

8. The soil filling culture material for fruiting Dictyophora rubrovalvata is characterized by comprising the following components in 1000 g: 350-500 g of pigeon pea sawdust, 300-400 g of longan sawdust, 100-150 g of pigeon pea leaves, 80-120 g of bagasse, 8-12 g of gypsum powder, 8-12 g of calcium superphosphate, 200ml of the growth promoter according to any one of claims 1-3 and 400-350ml of water.

9. The use of the growth promoter according to any one of claims 1 to 3 for promoting the growth of dictyophora rubrovolvata, shortening the growth cycle of dictyophora rubrovolvata, and improving the quality of dictyophora rubrovolvata hyphae.

10. A method for culturing Dictyophora rubrovalvata comprises the following steps:

1) inoculating Dictyophora rubrovalvata mother strain to stock culture medium, and culturing to obtain solid stock;

2) inoculating the solid stock into a culture medium for culturing to obtain a culture;

3) inoculating the cultivated species into a fruiting culture medium to culture to obtain a fruiting bag full of mycelia;

4) and moving the fruiting bags to a fruiting shed, peeling off the plastic bags, flatly placing the plastic bags on the sterilized ground, filling soil culture materials between the fruiting bags, and covering 2-5cm of soil on the fruiting bags for fruiting management.

11. The culture method according to claim 10, wherein in the step 4), the fruiting temperature is 22-28 ℃, and the air humidity of fruiting is 80-90%; in the fruiting process, water is sprayed once every 5-7 days before the fruiting buds of the Dictyophora rubrovalvata come out of the soil; when more than 50% of Dictyophora rubrovalvata has formed mushroom buds, spraying water once every 3 days; 4-5 tide mushrooms can be picked, and the interval of every tide of mushrooms is 20-25 days.

Technical Field

The invention belongs to the technical field of edible fungus cultivation, and particularly relates to a Dictyophora rubrovalvata growth promoter, and a preparation method and application thereof.

Background

Dictyophora rubrovolvata (Dictyophora rubrovolvata) belongs to Basidiomycota (Basidiomycotina), Abdominal class (Gasteromycetes), Coprinales (Phallales), Coprinaceae (Phallaceae) and Dictyophora (Dictyophora), is a moderate-temperature type rare edible fungus integrating medicinal and edible purposes in areas such as Guizhou and Yunnan provinces, and has the mushroom growing suitable temperature of 18-28 ℃. The dictyophora rubrovolvata fungus has the advantages of fleshy flesh, rich nutrition, delicious taste and higher edible and economic values, and is a top-quality product in edible fungi. Due to the high nutritional and medicinal values of dictyophora rubrovolvata, the market demand for the dictyophora rubrovolvata is high, the retail price of the current fresh dictyophora rubrovolvata fungus flowers (fruiting bodies of the opened umbrellas) in the market reaches 120-130 yuan/jin of the market, and the fungus eggs reach 80-100 yuan/jin of the market.

The most urgent problem in dictyophora rubrovolvata production is that the production period is long due to slow growth of dictyophora rubrovolvata hypha. The average growth speed of dictyophora rubrovolvata hyphae is 0.08-0.10 cm/day, the time required for a producer to culture an original seed, a cultivation bag and a fruiting bag is 90-100 days, 100-110 days and 100-110 days respectively, and the whole time from the original seed to the fruiting bag is up to 9-10 months. The consequences of slow hyphal growth are: (1) the growth cycle is too long; (2) aging of strains caused by the age of the strains; (3) high cost and low yield caused by overlong culture process; (3) the fruiting bags produced by the strains are not only slow in recovery growth due to uneven fungus age and aging, but also further prolong the time for hyphae to overgrow the bags, and meanwhile, the hyphae have low resistance to adverse environments, the yield of the fruiting bags is low, the yield is poor, the yield is uneven, and the income of mushroom farmers and the positivity of planting are seriously influenced.

Disclosure of Invention

In view of the above, the present invention aims to provide a dictyophora rubrovolvata growth promoter, and a preparation method and an application thereof. The growth promoter can promote the growth of dictyophora rubrovolvata hyphae, shorten the growth period of the dictyophora rubrovolvata and improve the yield.

In order to achieve the above purpose, the invention provides the following technical scheme:

the invention provides a growth promoter for Dictyophora rubrovalvata, wherein each liter of the growth promoter comprises 850-950 mL of lucid ganoderma fermentation liquid product, 200-300 g of medlar powder and 1.6-2.0 g of forchlorfenuron.

Preferably, the ganoderma lucidum fermentation liquor product is prepared by the following method: inoculating the solid ganoderma lucidum mother strain into a primary fermentation culture medium, culturing at 25-28 ℃ for 4-6 days to obtain a primary liquid strain, and transferring the primary liquid strain to a secondary fermentation culture medium for culturing for 5-6 days to obtain a secondary liquid fermentation product.

Preferably, the primary fermentation medium comprises the following components in 1000 g: 180-220 g of crushed folium cercis negundo, 180-220 g of dried Chinese yam slices, 30-40 g of milk powder, 20-30 g of pigeon pea powder, 2.0-3.0 g of yeast powder, 2.0-3.0 g of cellulose, 0.08-0.12 g of aspergillus oryzae bacterial powder, 0.08-0.12 g of bacillus bacterial powder, 1.5-2.5 g of monopotassium phosphate, 1.0-2.0 g of magnesium sulfate, 20-30 g of glucose and 0.5mL of tween-800.4;

the secondary fermentation medium comprises the following components in 1000 g: 340-360 g of crushed folium cercis negundo, 110-130 g of dried rhizoma dioscoreae slices, 10-20 g of milk powder, 10-20 g of pigeon pea powder, 2.0-3.0 g of yeast powder, 1.0-2.0 g of cellulose, 0.08-0.12 g of aspergillus oryzae bacterial powder, 0.08-0.12 g of bacillus bacterial powder, 1.5-2.5 g of monopotassium phosphate, 1.0-2.0 g of magnesium sulfate, 20-30 g of glucose and 0.3-0.5 mL of food antifoaming agent.

The invention provides a preparation method of the growth promoter, which comprises the following steps: and mixing the lucid ganoderma fermentation liquid product, the medlar powder and the forchlorfenuron, and heating at 95-100 ℃ for 25-35 min to obtain the growth promoter.

The invention provides a Dictyophora rubrovalvata stock culture medium which comprises the following components in 1000 g: 350-450 g of cotton seed hulls, 150-250 g of cajan leaves, 200-300 g of cajan sawdust, 120-140 g of bagasse, 8-12 g of gypsum powder, 8-12 g of calcium superphosphate, 200-300 ml of growth promoter and 300-450ml of water.

The invention provides a Dictyophora rubrovalvata culture medium, which comprises the following components in 1000 g: 350-450 g of cotton seed hulls, 100-200 g of cajan leaves, 200-260 g of cajan sawdust, 80-120 g of cassava dregs, 80-120 g of bagasse, 8-12 g of gypsum powder, 8-12 g of calcium superphosphate, 250ml of growth promoters and 300-350ml of water.

The invention provides a dictyophora rubrovolvata fruiting bag culture medium which comprises the following components in 1000 g: 400-500 g of pigeon pea sawdust, 200-250 g of longan sawdust, 120-140 g of pigeon pea leaves, 80-120 g of cotton seed hulls, 70-90 g of bagasse, 8-12 g of gypsum powder, 8-12 g of calcium superphosphate, 200ml of growth promoter and 350ml of water 400-.

The invention provides a soil filling culture material for fruiting Dictyophora rubrovalvata, which comprises the following components in 1000 g: 350-500 g of pigeon pea sawdust, 300-400 g of longan sawdust, 100-150 g of pigeon pea leaves, 80-120 g of bagasse, 8-12 g of gypsum powder, 8-12 g of calcium superphosphate, 200ml of growth promoter and 400-350ml of water.

The invention provides application of the growth promoter in promoting growth of dictyophora rubrovolvata, shortening growth period of the dictyophora rubrovolvata and improving quality of dictyophora rubrovolvata hypha.

The invention provides a method for culturing Dictyophora rubrovalvata, which comprises the following steps:

1) inoculating Dictyophora rubrovalvata stock to stock culture medium, and culturing to obtain solid stock;

2) inoculating the solid stock into a culture medium for culturing to obtain a culture;

3) inoculating the cultivated species into a mushroom culture medium to culture to obtain mushroom bags;

4) and (4) moving the fruiting bags out, stripping plastic bags on the surfaces of the fruiting bags, and placing the plastic bags on the sterilized ground, wherein the distance between the fruiting bags is 8-10 cm. And after filling soil culture materials between the fruiting bags, covering 2-5cm of soil on the fruiting bags for fruiting.

Preferably, the fruiting temperature in the step 4) is 22-28 ℃, and the air humidity of fruiting is 80-90%; in the fruiting process, water is sprayed once every 5-7 days before the fruiting buds of the Dictyophora rubrovalvata come out of the soil; when more than 50% of Dictyophora rubrovalvata has formed mushroom buds, water is sprayed once every 3 days.

The invention has the beneficial effects that: the growth promoter for Dictyophora rubrovalvata provided by the invention comprises a lucid ganoderma fermentation liquid product, medlar powder and forchlorfenuron; the combination of the ganoderma lucidum fermentation liquor product, the plant hormone and the polysaccharide promotes the growth of hypha, and achieves a very good effect.

The growth promoter provided by the invention can promote the growth of dictyophora rubrovolvata hyphae, shorten the growth period of the dictyophora rubrovolvata and improve the yield. According to the records of the embodiments, when the growth promoter and the method provided by the invention are used for culturing dictyophora rubrovolvata, the hypha growth speed of the dictyophora rubrovolvata is increased to 0.70-0.75 cm/d, and the time for the hypha of the stock species, the cultivated species and the fruiting bag of the dictyophora rubrovolvata to grow over the fungus bag is respectively shortened to 25-30 d, 30-35 d and 30-35 d. The method for culturing dictyophora rubrovolvata has the advantages that the biological conversion rate is improved, the yield of the dictyophora rubrovolvata reaches 1990-2011 kg/mu, and the biological conversion rate is 56-58%; the culture method of dictyophora rubrovolvata has the advantages that the yield of mushroom bags is improved, the yield of mushroom bag production is 98.7%, and is improved by 9.1% compared with a contrast.

Detailed Description

The invention provides a growth promoter for Dictyophora rubrovalvata, wherein each liter of the growth promoter comprises 850-950 mL of lucid ganoderma fermentation liquid product, 200-300 g of medlar powder and 1.6-2.0 g of forchlorfenuron.

In the invention, each liter of the growth promoter preferably comprises 900mL of lucid ganoderma fermentation liquid product, 250g of medlar powder and 1.8g of forchlorfenuron. The sources of the medlar powder and the forchlorfenuron are not particularly limited, and the medlar powder and the forchlorfenuron can be prepared from conventional commercial products in the field.

In the invention, the ganoderma lucidum fermentation liquor product is preferably prepared by the following method: inoculating the solid ganoderma lucidum mother seeds into a primary fermentation culture medium, culturing for 4-6 days at 25-28 ℃ to obtain liquid mother seeds, and transferring the liquid mother seeds to a secondary fermentation culture medium for culturing for 5-6 days to obtain the ganoderma lucidum solid mother seeds.

In the present invention, the primary fermentation medium, in 1000g, preferably comprises the following components: 180-220 g of crushed folium cercis negundo, 180-220 g of dried Chinese yam slices, 30-40 g of milk powder, 20-30 g of pigeon pea powder, 2.0-3.0 g of yeast powder, 2.0-3.0 g of cellulose, 0.08-0.12 g of aspergillus oryzae bacterial powder, 0.08-0.12 g of bacillus bacterial powder, 1.5-2.5 g of monopotassium phosphate, 1.0-2.0 g of magnesium sulfate, 20-30 g of glucose and 0.5mL of Tween-800.4; more preferably, the following components are included: 200g of folium cercis negundo crushed material, 200g of Chinese yam dry tablet, 35g of milk powder, 25g of pigeon pea powder, 2.5g of yeast powder, 2.5g of cellulose, 0.1g of aspergillus oryzae bacterial powder, 0.1g of bacillus bacterial powder, 2.0g of monopotassium phosphate, 1.5g of magnesium sulfate, 25.0g of glucose and 800.5mL of tween-80. In the invention, the pigeon pea (Cajanus cajan) is a perennial bean plant which is cultivated in most in Guangxi in recent years, has nitrogen fixation effect, the seed of the pigeon pea contains rich protein, and the leaf of the pigeon pea contains more cellulose and has higher nitrogen content; the pigeon pea is used in the primary fermentation culture medium, so that the yield of ganoderma lucidum fermentation liquid products can be increased, the value of pigeon pea can be increased, and wastes can be changed into valuables.

In the invention, the primary fermentation medium is used after being sterilized, and the solid ganoderma lucidum mother seeds are preferably solid ganoderma lucidum mother seeds with the density of 1.0cm multiplied by 1.0 cm; the temperature of the primary fermentation culture is preferably 26-27 ℃, and the time of the primary fermentation culture is preferably 5 d; in the primary fermentation culture process, stirring is preferably carried out, and the rotating speed of the stirring is preferably 120-130 rpm, more preferably 125 rpm; after the primary fermentation culture is finished, the primary liquid strain with the hypha density of more than 80% of the culture solution is preferably obtained.

In the invention, the primary fermentation medium is counted by 1L, and the preparation method comprises the following steps: mixing folium Cercis chinensis pulverized material, rhizoma Dioscoreae dry pieces, semen Cajani powder, Aspergillus oryzae powder, Bacillus subtilis powder, cellulose, and 700mL of water, shake culturing the mixture for a certain time, heating and boiling, maintaining for 10min, filtering, removing residue, and collecting filtrate; mixing milk powder, yeast powder, potassium dihydrogen phosphate, magnesium sulfate, glucose and tween-80 with the filtrate, stirring to dissolve, and adding water to desired volume of 1L. In the invention, the rotation speed of the shaking culture is preferably 120rpm, and the time of the shaking culture is preferably 20-24 h.

After the primary liquid strain is obtained, the primary liquid strain is transferred to a secondary fermentation medium for culture for 5-6 days. In the present invention, the secondary fermentation medium preferably comprises the following components in an amount of 1000 g: 340-360 g of crushed folium cercis negundo, 110-130 g of dried Chinese yam slices, 10-20 g of milk powder, 10-20 g of pigeon pea powder, 2.0-3.0 g of yeast powder, 1.0-2.0 g of cellulose, 0.08-0.12 g of aspergillus oryzae bacterial powder, 0.08-0.12 g of bacillus bacterial powder, 1.5-2.5 g of monopotassium phosphate, 1.0-2.0 g of magnesium sulfate, 20-30 g of glucose and 0.3-0.5 mL of food antifoaming agent; more preferably, the food comprises 350g of the crushed folium cercis chinensis, 120g of Chinese yam dry slices, 15g of milk powder, 15g of pigeon pea powder, 2.5g of yeast powder, 1.5g of cellulose, 0.1g of aspergillus oryzae bacterial powder, 0.1g of bacillus bacterial powder, 2.0g of monopotassium phosphate, 1.5g of magnesium sulfate, 25.0g of glucose and 0.4mL of food antifoaming agent.

In the invention, the secondary fermentation medium is counted by 1L, and the preparation method comprises the following steps: mixing folium Cercis chinensis pulverized material, rhizoma Dioscoreae dry pieces, semen Cajani powder, Aspergillus oryzae powder, Bacillus subtilis powder, cellulose, and 700mL of water, shake culturing the mixture for a certain time, heating and boiling, maintaining for 10min, filtering, removing residue, and collecting filtrate; mixing milk powder, yeast powder, potassium dihydrogen phosphate, magnesium sulfate, glucose, food antifoaming agent and the filtrate, stirring for dissolving, and adding water to desired volume of 1L. In the invention, the rotation speed of the shaking culture is preferably 120rpm, and the time of the shaking culture is preferably 20-24 h. In the present invention, the secondary fermentation medium is used after sterilization; in the present invention, the secondary fermentation culture is preferably carried out in a fermentor, and the inoculation amount of the liquid mother culture is preferably 0.5% to 0.7%, more preferably 0.625%. In the invention, the temperature of the secondary fermentation culture is preferably 28 ℃, the pressure of the fermentation tank is preferably 0.03-0.05MPa, and the pressure gauge of the air pump is 0.15 MPa. The invention obtains the ganoderma lucidum fermentation liquor product with the mycelium pellet density of 80 percent after the secondary fermentation culture is finished.

The invention provides a preparation method of the growth promoter, which comprises the following steps: and mixing the lucid ganoderma fermentation liquid product, the medlar powder and the forchlorfenuron, and heating at 95-100 ℃ for 25-35 min to obtain the growth promoter. In the present invention, the heating time is preferably 30 min; the growth promoter is preferably stored at 4-10 ℃ for later use.

The invention provides application of the growth promoter in promoting growth of dictyophora rubrovolvata, shortening growth period of the dictyophora rubrovolvata and improving quality of dictyophora rubrovolvata hypha.

The invention also provides a method for culturing Dictyophora rubrovalvata, which comprises the following steps: 1) inoculating Dictyophora rubrovalvata mother strain to stock culture medium, and culturing to obtain solid stock; 2) inoculating the solid stock into a culture medium for culturing to obtain a culture; 3) inoculating the cultivated species into a mushroom culture medium to culture to obtain mushroom bags; 4) and (4) moving the fruiting bags out, stripping plastic bags on the surfaces of the fruiting bags, and placing the plastic bags on the sterilized ground, wherein the distance between the fruiting bags is 8-10 cm. Filling soil filling compost between the fruiting bags, and then covering 2-5cm of soil on the fruiting bags for fruiting management; the stock culture medium, the cultivated species culture medium, the mushroom culture medium and the soil filling culture medium comprise the growth promoter.

In the invention, Dictyophora rubrovalvata mother strain is inoculated into a stock culture medium to be cultured to obtain a solid stock. In the present invention, the stock culture medium comprises the following components in 1000 g: 350-450 g of cotton seed hulls, 150-250 g of cajan leaves, 200-300 g of cajan sawdust, 120-140 g of bagasse, 8-12 g of gypsum powder, 8-12 g of calcium superphosphate, 200-300 ml of growth promoter and 300-450ml of water, and preferably comprises the following components: 400g of cotton seed hulls, 200g of cajan leaves, 250g of cajan sawdust, 130g of bagasse, 10g of gypsum powder, 10g of calcium superphosphate, 250ml of growth promoter and 300-450ml of water, wherein the water content of the stock culture medium is preferably 60-65%. After the stock culture medium is obtained, the stock culture medium is subpackaged in polypropylene plastic bags, sterilized and cooled, and then the stock is inoculated; the mother seed is preferably a solid of 1.0cm × 1.0cm × 1.0cm size; the temperature of stock culture is preferably 24-28 ℃, and solid stock is obtained after the stock culture is finished until hyphae grow over a culture medium.

The solid stock is inoculated into a culture medium of a cultivated species for cultivation to obtain the cultivated species. The temperature of the cultivated species is preferably 24-28 ℃, and the cultivated species is obtained after the hypha grows over the culture medium. In the invention, the culture medium comprises the following components in 1000 g: 350-450 g of cotton seed hulls, 100-200 g of cajan leaves, 200-260 g of cajan sawdust, 80-120 g of cassava dregs, 80-120 g of bagasse, 8-12 g of gypsum powder, 8-12 g of calcium superphosphate, 250ml of growth promoter and 300-350ml of water, and preferably comprises the following components: 400g of cotton seed hulls, 150g of cajan leaves, 230g of cajan sawdust, 100g of cassava dregs, 100g of bagasse, 10g of gypsum powder, 10g of calcium superphosphate, 250ml of growth promoter and 300-350ml of water, wherein the water content of the culture medium for the cultivated species is preferably 60-65%. The temperature of the cultivated species is preferably 24-28 ℃, and the cultivated species is obtained after the hypha grows over the culture medium.

The invention inoculates the cultivated species into a mushroom culture medium to culture to obtain mushroom bags. In the invention, the mushroom culture medium is counted by 1000g and comprises the following components: 400-500 g of pigeon pea sawdust, 200-250 g of longan sawdust, 120-140 g of pigeon pea leaves, 80-120 g of cotton seed hulls, 70-90 g of bagasse, 8-12 g of gypsum powder, 8-12 g of calcium superphosphate, 200ml of growth promoter and 350ml of water 400-; preferably comprising the following components: 450g of pigeon pea sawdust, 220g of longan sawdust, 130g of pigeon pea leaves, 100g of cottonseed hulls, 80g of bagasse, 10g of gypsum powder, 10g of calcium superphosphate, 200ml of growth promoter and 350ml of water 400, and the water content of the mushroom culture medium is preferably 60-65%. In the invention, the temperature for culturing the fruiting bag is 20-26 ℃, and the fruiting bag is cultured until hypha grows full of the culture medium to obtain the mushroom bag.

The mushroom growing bags are moved out, plastic bags on the surfaces of the mushroom growing bags are stripped, the mushroom growing bags are placed on the sterilized ground, and the space between the mushroom growing bags is 8-10 cm. And after filling soil culture materials between the fruiting bags, covering 2-5cm of soil on the fruiting bags for fruiting. In the invention, the fruiting temperature is preferably 22-28 ℃, and the air humidity for fruiting is preferably 80-90%. In the fruiting process, preferably, water is sprayed once every 5 to 7 days before the fruiting buds of the dictyophora rubrovolvata come out; when more than 50% of Dictyophora rubrovalvata has formed mushroom buds, water is sprayed once every 3 days.

The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.