阅读说明：本技术 一种含墨角藻提取液和甘油葡萄糖苷的组合物 (A composition containing Fucus vesiculosus extract and glycerol glucoside ) 是由 金巍伟 黄爱清 林彦斌 于 2019-10-24 设计创作，主要内容包括：本发明公开了含墨角藻提取液和甘油葡萄糖苷的组合物。本发明的制备含墨角藻提取液和甘油葡萄糖苷的组合物的方法包括分别制备墨角藻提取液和甘油葡萄糖苷,然后将这两种原料按照一定比例进行混合,墨角藻提取液与甘油葡萄糖苷最适比例为3：1,保湿效果良好。(The invention discloses a composition containing a fucus extract and glycerol glucoside. The method for preparing the composition containing the fucus extract and the glycerol glucoside comprises the steps of respectively preparing the fucus extract and the glycerol glucoside, and then mixing the two raw materials according to a certain proportion, wherein the optimal proportion of the fucus extract to the glycerol glucoside is 3: 1, the moisturizing effect is good.)

1. A composition containing an extract of Fucus vesiculosus and a glyceroglucoside, comprising: preparing single components of the fucus extract and the glycerol glucoside, and mixing the components.

2. The composition of claim 1 comprising a fucus extract and a glycerol glucoside, comprising: the dried fucus vesiculosus powder is 1.0-3.0% (wt%, weight percentage concentration), the used solvent is ethanol, the used concentration is 40-70% (wt%, weight percentage concentration), the extraction heating is carried out for 50-70 ℃, and the reflux extraction is carried out for 5-8 hours. The extract is then recovered by filtration equipment. The extract is concentrated and the ethanol is removed. And then adding active carbon for decolorization, wherein the concentration of the active carbon is 0.5-1.5% (wt%, weight percentage concentration), the decolorization temperature is 70 ℃, and the decolorization time is 1 hour. And after the decolorization is finished, filtering to obtain decolorized liquid. Finally, 0.5% (v/v) caprylyl hydroximic acid and 1.5% (v/v)1, 2-hexanediol are added as preservatives according to the volume ratio of the destaining solution.

3. The fucus extract-containing composition according to claim 2, wherein the preferred characteristics comprise: dried Fucus vesiculosus powder 1.5% (wt%, wt% concentration), the solvent used was ethanol, which was used at a concentration of 50% (wt%, wt% concentration), and extraction was carried out at 60 ℃ and reflux extraction was carried out for 6 hours. The extract is then recovered by filtration equipment. The extract is concentrated and the ethanol is removed. Then adding active carbon for decoloring, wherein the concentration of the active carbon is 0.5-1.5% (wt%, weight percentage concentration).

4. The composition of claim 1 comprising a fucus extract and a glycerol glucoside, comprising: dissolving glycerol and glycosyl donor into a buffer solution, wherein the concentration of the glycerol is 5.0-9.2% (wt%, weight percentage concentration), and the concentration of the glycosyl donor is 18-34%. Carrying out enzyme catalysis synthesis on the glycerol glucoside at the temperature of 30-55 ℃, wherein the catalytic reaction time is 24-48 hours. The glycoside donor herein includes glucose, maltose, sucrose, and the glycosyl converting enzyme includes a-glucosidase, maltose phosphorylase, sucrose phosphorylase.

5. The fucus extract-containing composition according to claim 4, wherein the preferred characteristics comprise: a preferred combination of glycerol with a glycosyl donor and a glycosyltransferase is glycerol-maltose phosphorylase. The preferred concentration of glycerol is 7.5% (wt%, concentration by weight); maltose, preferably at a concentration of 34% (wt%, concentration by weight); the dosage of the maltose phosphorylase is 1500U/L; preferably, the enzyme catalysis temperature is 40 ℃ and the enzyme catalysis time is 24 h.

6. The composition of claim 1 comprising a fucus extract and a glycerol glucoside, comprising: the ratio of the fucus extract to the glycerol glucoside comprises 1: 1. 2: 1. 3: 1. 4: 1. 1: 2. 1: 3. 1: 4; the preferable mixture ratio is 2: 1 and 3: 1; the most preferable mixture ratio is 3: 1.

Technical Field

The invention relates to a preparation method of a composition of a fucus extract and glycerol glucoside, belonging to the field of cosmetics.

Background

Fucus vesiculosus (Fucus vesiculosus) is an obligate marine brown algae growing on rock attached to growing seaside, and its algae body is black brown, and its growth form is bifurcate branch, and is a flat horn-shaped leaf. Fucus vesiculosus extract is rich in fucoidan polysaccharide components, and also contains bioactive substances such as mucoid, algin, mannitol, beta-carotene, zeaxanthin and brown algae polyphenol. Fucoidan (figure 1) is an important fucoidan active substance in Fucus vesiculosus extractive solution, and has biological activities of regulating immunity, promoting cell growth, migration and adhesion, resisting virus, resisting blood coagulation and ascorbic acid, and scavenging free radicals. The Fucus vesiculosus extractive solution has effects of shrinking collagen gel, resisting aging and wrinkle, and keeping moisture.

Glycerol Glucoside (glycocide) is a substance formed by combining glycerol molecules and glucose molecules through glycosidic bonds, and the molecular structure of the glycerol Glucoside is shown in fig. 2:

it is a natural cell activator, is commonly used as a cosmetic raw material, and has the following functions: can protect the cell membrane and the cell structure from invariance and damage; the water-retaining agent has excellent water-retaining capacity, and water still exists in cells in an extremely dry environment; small molecular weight and easy absorption.

Disclosure of Invention

The invention aims to provide a composition containing a fucus extract and glycerol glucoside, which has the characteristics of good moisturizing effect, tenderness and whiteness, skin wrinkle and the like.

The invention can be realized by the following technical scheme:

the invention discloses a composition containing Fucus vesiculosus extract and glycerol glucoside, which comprises 60-80% (v/v, volume percentage) of the Fucus vesiculosus extract and 20-40% (v/v, volume percentage) of the glycerol glucoside.

Furthermore, the preparation method of the fucus extract comprises the following steps: selecting dry fucus vesiculosus powder, adding 40-70% (wt%, wt% concentration) ethanol according to 1.0-3.0% (wt%, wt% concentration), mixing uniformly, heating to 50-70 ℃, and performing reflux extraction for 5-8 hours. The extract is then recovered by filtration equipment. The extract is concentrated and the ethanol is removed. Then 0.5-1.5% (wt%, weight percentage concentration) of activated carbon is added into the concentrated solution, and the solution is heated to 70 ℃ for decolorization for 1 hour. And after the decolorization is finished, filtering to obtain decolorized liquid. Finally, 0.5% (v/v) caprylyl hydroximic acid and 1.5% (v/v)1, 2-hexanediol are added as preservatives according to the volume ratio of the destaining solution.

Preferably, the preparation method of the fucus extract comprises the following steps: selecting dry fucus, adding 50-60% (wt%, weight percentage concentration) ethanol according to the calculation of 1.5-2.0% (wt%, weight percentage concentration), uniformly mixing, heating to 55-60 ℃, and performing reflux extraction for 6-7 hours. The extract is then recovered by filtration equipment. The extract is concentrated and the ethanol is removed. Then 0.8-1.0% (wt%, weight percentage concentration) of activated carbon is added into the concentrated solution, and the solution is heated to 70 ℃ for decolorization for 1 hour. And after the decolorization is finished, filtering to obtain decolorized liquid. Finally, 0.5% (v/v) caprylyl hydroximic acid and 1.5% (v/v)1, 2-hexanediol are added as preservatives according to the volume ratio of the destaining solution.

Further, the preparation method of the glycerol glucoside comprises the following steps: dissolving 5.0-9.2% (wt%, weight percentage concentration) of glycerol and 18-34% (wt%, weight percentage concentration) of glycosyl donor in a buffer solution, adding 1500U/L glycosyltransferase, and carrying out enzyme catalysis synthesis at the temperature of 30-55 ℃ for 24-48 hours. After the reaction is finished, raising the reaction temperature to 60-80 ℃, adjusting the pH value to 5.0, preserving the temperature for 30 minutes to inactivate the enzyme to terminate the reaction, and then separating and purifying to obtain the solution containing the glycerol glucoside with the concentration of 50%. The glycoside donor herein includes glucose, maltose, sucrose, and the glycosyl converting enzyme includes a-glucosidase, maltose phosphorylase, sucrose phosphorylase.

Drawings

FIG. 1 is a molecular structure diagram of fucoidan sulfate provided by the present application

FIG. 2 is a diagram of the molecular structure of glycerol glucoside provided by the present application

Detailed Description

The present invention is described in further detail below with reference to specific embodiments to help those skilled in the art to further understand the innovations of the present invention.