Active cell microalgae repairing liquid and preparation method thereof

文档序号:1623514 发布日期:2020-01-14 浏览:6次 中文

阅读说明:本技术 一种活性细胞微藻修复液及其制备方法 (Active cell microalgae repairing liquid and preparation method thereof ) 是由 杨新年 段鹏程 赵杰 于 2019-10-10 设计创作,主要内容包括:本发明公开了一种活性细胞微藻修复液,具体涉及农业技术领域,包含多种呈活体状态的微藻,按微藻活性细胞个体数量计,添加的微藻包括有红毛藻10-50份、蓝藻5-40份、轮门藻10-30份、金鱼藻30-50份,且按质量份数计;本发明还公开了上述微藻修复液的制备方法,包括如下操作步骤:步骤一:分别扩大培植红毛藻、蓝藻、轮门藻和金鱼藻,得到四种微藻液,备用。本发明通过在微藻液中加入植物生长调节剂、藻类提取物和硒、锌、铁元素提取物,不仅能够提高土壤的肥效,并且具有杀虫、杀菌作用,可灭杀土壤内部以及表面的细菌,提高土壤的分解能力,分解出有机质,从而使得土壤修复效率高。(The invention discloses an active cell microalgae repairing liquid, which particularly relates to the technical field of agriculture, and comprises a plurality of microalgae in a living state, wherein the added microalgae comprise 10-50 parts of Haematococcus, 5-40 parts of blue algae, 10-30 parts of Phlomis verticillata and 30-50 parts of Goldfish algae according to the individual number of active cells of the microalgae, and the added microalgae comprise the following components in parts by weight; the invention also discloses a preparation method of the microalgae repairing liquid, which comprises the following operation steps: the method comprises the following steps: respectively culturing red hair algae, blue algae, stonewort and goldfish algae to obtain four kinds of microalgae solutions for later use. According to the invention, the plant growth regulator, the algae extract and the selenium, zinc and iron element extracts are added into the microalgae liquid, so that the fertilizer efficiency of the soil can be improved, the soil has the effects of killing insects and bacteria, killing bacteria in the soil and on the surface of the soil, improving the decomposition capability of the soil and decomposing organic matters, and the soil remediation efficiency is high.)

1. An active cell microalgae repairing liquid is characterized in that: the biological microalgae culture medium comprises a plurality of microalgae in a living state, wherein the added microalgae comprise 10-50 parts of Haematococcus, 5-40 parts of blue algae, 10-30 parts of stonewort and 30-50 parts of Goldfish algae according to the individual number of active cells of the microalgae, and 5 parts of a plant growth regulator is also added according to the parts by weight.

2. The active cell microalgae repairing fluid as claimed in claim 1, which is characterized in that: according to the individual quantity of the microalgae active cells, the added microalgae comprise 5-35 parts of the red hair algae, 10-30 parts of the blue algae, 10-25 parts of the stonewort and 35-50 parts of the goldfish algae.

3. The active cell microalgae repairing fluid as claimed in claim 1, which is characterized in that: the plant growth regulator in the raw materials comprises three types of plant growth regulators for improving amino acid content, protein content and stress resistance, and the plant growth regulator for improving the amino acid content comprises the following components: plant growth regulator for increasing protein content: stress-resistance-improving plant growth regulators =1: 2.

4. the active cell microalgae repairing fluid as claimed in claim 3, which is characterized in that: the plant growth regulator for increasing the amino acid content is prepared by mixing paclobutrazol, atrazine and indole mature ester, the plant growth regulator for increasing the protein content is prepared by mixing atrazine, simazine, atrazine and naphthylacetic acid, and the plant growth regulator for increasing the stress resistance is prepared by mixing abscisic acid, paclobutrazol, daminozide and chlormequat chloride.

5. The active cell microalgae repairing fluid as claimed in claim 1, which is characterized in that: the preparation raw materials of the repairing liquid also comprise algae extracts and selenium, zinc and iron element extracts, wherein the algae raw materials in the algae extracts are the bifidobacterium brevicornum, the chlorella vulgaris and the anabaena, and the parent raw materials of the selenium, zinc and iron element extracts are oyster mushroom, oyster meat and sedum.

6. A method for preparing the active cell microalgae repairing liquid according to any one of claims 1 to 5, which is characterized by comprising the following steps:

the method comprises the following steps: respectively carrying out expanded cultivation on the Rhodophyta, the blue algae, the stonewort and the Goldfish algae to obtain four microalgae solutions for later use;

step two: preparing three types of plant growth regulators capable of improving the amino acid content, the protein content and the stress resistance for later use;

step three: preparing algae extract and selenium, zinc and iron element extract for later use;

step four: and (3) fully mixing the four microalgae solutions in the steps with a plant growth regulator, the algae extract and the selenium, zinc and iron element extracts in sequence to obtain the active cell microalgae repairing solution.

7. The method for preparing the active cell microalgae repairing liquid as claimed in claim 6, which is characterized in that: the culture medium solution for expanding culture in the first step comprises the following components in concentration: 2-6mg/L disodium ethylene diamine tetraacetate, 5-13mg/L citric acid and 0.05-0.15mg/L FeSO4•7H2O、0.15-0.35g/L NaHCO3、0.03-0.1g/L CaCl2、0.05-0.2g/L MgSO4•7H2O、1-3.5g/L NaNO3、0.05-0.15g/L K2HPO4、0.2-1g/L KH2PO4、1.5-2.5mg/L MnCl2•4H2O、0.02-0.05mg/L ZnSO4•7H2O、0.05-0.1mg/L CuSO4•5H2O、0.02-0.05mg/L Na2MoO4•2H2O、2-4mg/L H3BO3

8. The method for preparing the active cell microalgae repairing liquid as claimed in claim 6, which is characterized in that: the expanding cultivation process in the first step comprises the following steps: selecting and separating single microalgae cells, and carrying out hierarchical expansion cultivation on the single microalgae cells, wherein in each stage of expansion cultivation process, when the number of the microalgae cells is more than or equal to 106/mL, the next stage of expansion cultivation process is carried out, namely the single microalgae cells are cultivated in 15mL of culture medium solution for 10-15 days, 15mL of algae solution → transfer inoculation is carried out in 85mL of culture medium solution for continuous cultivation for 10-15 days, 100mL of algae solution is obtained → transfer inoculation is carried out in 0.9L of culture medium solution for continuous cultivation for 10-15 days, 1L of algae solution → transfer inoculation is carried out in 4L of culture medium solution for continuous cultivation for 10-15 days, 5L of algae solution is obtained → transfer inoculation is carried out in 13L of culture medium solution for continuous cultivation for 10-15 days, 18L of algae solution → transfer inoculation is carried out in 782L of culture medium solution for continuous cultivation for 10-15 days, and 800L of algae solution is obtained.

9. The method for preparing the active cell microalgae repairing liquid as claimed in claim 6, which is characterized in that: the extraction process of the algae extract in the third step comprises the following steps: centrifuging and collecting algae cells of Bifidobacterium minutissima, Chlorella vulgaris and Anabaena for 3 times, continuously washing with distilled water, lyophilizing at-50 deg.C, storing for use, weighing appropriate amount of lyophilized powder of algae of three kinds, and adding appropriate amount of PBS buffer solution with pH of 7.0 to obtain algae extract with density of 1.0 g/L.

10. The method for preparing the active cell microalgae repairing liquid as claimed in claim 6, which is characterized in that: the extraction process of the selenium, zinc and iron element extracts in the three steps comprises the steps of respectively mashing oyster mushroom, oyster meat and sedum aizoon, centrifuging and filtering to obtain filtrate, namely the corresponding extracts.

Technical Field

The invention relates to the technical field of agriculture, in particular to an active cell microalgae repairing liquid and a preparation method thereof.

Background

When the soil fertility level can not reach the nutrients required by plant growth, the soil needs to be fertilized, chemical fertilizer is widely used at present, but the negative effects brought by the application of the chemical fertilizer cannot be ignored: the raw materials for producing the chemical fertilizer contain heavy metals such as Zn, Cu, Co, Cr and the like, and the heavy metals are applied to the soil along with the chemical fertilizer, so that the heavy metal elements in the soil are enriched and enter the plant body through the absorption of the plant, and the growth of the plant and the quality of agricultural products and the like are influenced; the chemical fertilizer can reduce the quantity and activity of soil microorganisms, thereby reducing the conversion of organic matters in soil by the soil microorganisms, the decomposition of minerals and the degradation of toxic substances; the long-term application of chemical fertilizers can aggravate the acidification of soil, aggravate the hardening of the soil, inhibit the growth of plant roots and seriously reduce the stress resistance of plants.

Microalgae are autotrophic plants which are widely distributed in land soil, rivers and lakes and have high photosynthetic availability, are micro algae groups of which the forms can be distinguished under a microscope, are rich in nutrition, can generate nutrient substances such as polysaccharide and protein through cell metabolism, and have high application value in the fields of food, medicine, feed and the like.

However, the existing remediation liquid cannot kill bacteria in the soil and on the surface of the soil, and the soil remediation rate is low.

Disclosure of Invention

In order to overcome the above-mentioned drawbacks of the prior art, embodiments of the present invention provide an active cell microalgae repairing liquid and a preparation method thereof, in which a plant growth regulator, an algae extract, and selenium, zinc, and iron element extracts are added to a microalgae liquid, so that not only can the fertilizer efficiency of soil be improved, but also the microalgae repairing liquid has insecticidal and bactericidal effects, can kill bacteria in and on the surface of the soil, improve the decomposition capability of the soil, and decompose organic matters, so as to improve the soil repairing efficiency, thereby solving the problems in the background art.

In order to achieve the purpose, the invention provides the following technical scheme: the active cell microalgae repairing liquid comprises a plurality of microalgae in a living state, wherein the added microalgae comprise 10-50 parts of hirsutella, 5-40 parts of blue algae, 10-30 parts of stonewort and 30-50 parts of goldfish algae by mass of the individual number of active cells of the microalgae, and 5 parts of plant growth regulator is also added.

In a preferred embodiment, the added microalgae comprise 5-35 parts of red hair algae, 10-30 parts of blue algae, 10-25 parts of stonewort and 35-50 parts of goldfish algae in terms of the number of the individual microalgae active cells.

In a preferred embodiment, the plant growth regulator in the raw material for preparation comprises three types of plant growth regulators for increasing amino acid content, increasing protein content and increasing stress resistance, and the plant growth regulator for increasing amino acid content comprises: plant growth regulator for increasing protein content: stress-resistance-improving plant growth regulators =1: 2.

in a preferred embodiment, the amino acid content-increasing plant growth regulator is formed by mixing paclobutrazol, atrazine and indole mature ester, the protein content-increasing plant growth regulator is formed by mixing atrazine, simazine, atrazine and naphthylacetic acid, and the stress resistance-increasing plant growth regulator is formed by mixing abscisic acid, paclobutrazol, damycin and chlormequat chloride.

In a preferred embodiment, the raw materials for preparing the repairing liquid further include an algae extract and selenium, zinc and iron extracts, wherein the algae raw materials in the algae extract are the bifidobacterium unicum, the chlorella and the anabaena, and the parent raw materials of the selenium, zinc and iron extracts are the oyster mushroom, the oyster meat and the sedum.

The invention also provides a preparation method of the active cell microalgae repairing liquid, which comprises the following operation steps:

the method comprises the following steps: respectively carrying out expanded cultivation on the Rhodophyta, the blue algae, the stonewort and the Goldfish algae to obtain four microalgae solutions for later use;

step two: preparing three types of plant growth regulators capable of improving the amino acid content, the protein content and the stress resistance for later use;

step three: preparing algae extract and selenium, zinc and iron element extract for later use;

step four: and (3) fully mixing the four microalgae solutions in the steps with a plant growth regulator, the algae extract and the selenium, zinc and iron element extracts in sequence to obtain the active cell microalgae repairing solution.

In a preferred embodiment, the medium solution for expanding cultivation in the first step comprises the following components in concentration: 2-6mg/L disodium ethylene diamine tetraacetate, 5-13mg/L citric acid and 0.05-0.15mg/L FeSO4•7H2O、0.15-0.35g/L NaHCO3、0.03-0.1g/L CaCl2、0.05-0.2g/L MgSO4•7H2O、1-3.5g/L NaNO3、0.05-0.15g/L K2HPO4、0.2-1g/L KH2PO4、1.5-2.5mg/L MnCl2•4H2O、0.02-0.05mg/L ZnSO4•7H2O、0.05-0.1mg/L CuSO4•5H2O、0.02-0.05mg/L Na2MoO4•2H2O、2-4mg/L H3BO3

In a preferred embodiment, the expanding cultivation process in the first step comprises: selecting and separating single microalgae cells, and carrying out hierarchical expansion cultivation on the single microalgae cells, wherein in each stage of expansion cultivation process, when the number of the microalgae cells is more than or equal to 106/mL, the next stage of expansion cultivation process is carried out, namely the single microalgae cells are cultivated in 15mL of culture medium solution for 10-15 days, 15mL of algae solution → transfer inoculation is carried out in 85mL of culture medium solution for continuous cultivation for 10-15 days, 100mL of algae solution is obtained → transfer inoculation is carried out in 0.9L of culture medium solution for continuous cultivation for 10-15 days, 1L of algae solution → transfer inoculation is carried out in 4L of culture medium solution for continuous cultivation for 10-15 days, 5L of algae solution is obtained → transfer inoculation is carried out in 13L of culture medium solution for continuous cultivation for 10-15 days, 18L of algae solution → transfer inoculation is carried out in 782L of culture medium solution for continuous cultivation for 10-15 days, and 800L of algae solution is obtained.

In a preferred embodiment, the extraction process of the algae extract in the third step is as follows: centrifuging and collecting algae cells of Bifidobacterium minutissima, Chlorella vulgaris and Anabaena for 3 times, continuously washing with distilled water, lyophilizing at-50 deg.C, storing for use, weighing appropriate amount of lyophilized powder of algae of three kinds, and adding appropriate amount of PBS buffer solution with pH of 7.0 to obtain algae extract with density of 1.0 g/L.

In a preferred embodiment, the extraction process of the selenium, zinc and iron element extract in the third step is that oyster mushroom, oyster meat and sedum are respectively mashed, centrifuged and filtered, and the obtained filtrate is the corresponding extract.

The invention has the technical effects and advantages that:

compared with the existing microalgae repairing liquid, the microalgae active cell nutrient repairing liquid has the advantages that the plant growth regulator, the algae extract and the selenium, zinc and iron element extracts are added, so that the fertilizer efficiency of soil can be improved during actual use, the insecticidal and bactericidal effects are achieved, bacteria in and on the surface of the soil can be killed, the decomposition capability of the soil is improved, organic matters are decomposed, the soil repairing efficiency is high, the growth environment of crops is improved, and the yield is increased.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The invention provides an active cell microalgae repairing liquid which comprises a plurality of microalgae in a living state, wherein the added microalgae comprises 10-50 parts of hirsutella, 5-40 parts of blue algae, 10-30 parts of verticillium, 8-10 parts of rice hull extract, 5-8 parts of sweet potato extract, 6-9 parts of mixed bacteria extract and 30-50 parts of hornworts according to the individual number of active cells of the microalgae, and 5 parts of plant growth regulator is also added according to the parts by weight.

9页详细技术资料下载
上一篇:一种医用注射器针头装配设备
下一篇:一种基于水热炭肥的养分控释方法

网友询问留言

已有0条留言

还没有人留言评论。精彩留言会获得点赞!

精彩留言,会给你点赞!