阅读说明：本技术 一种酯类浓缩复合剂在提高苹果抵抗盐碱胁迫中的新应用 (Novel application of ester concentrated complexing agent in improving saline-alkali stress resistance of apples ) 是由 王彩虹 袭祥利 郑晓东 李玉琪 杜丽慧 于 2019-10-23 设计创作，主要内容包括：本发明涉及一种酯类浓缩复合剂在提高苹果抵抗盐碱胁迫中的新应用。以油菜素内酯和独角金内酯为有效成分的产品施于受盐碱胁迫的植株上,能显著增强植物抵抗盐碱胁迫的能力,减轻盐碱胁迫对植物造成的氧化伤害,从而缓解盐碱导致的产量和品质下降。(The invention relates to a new application of an ester concentrated complexing agent in improving saline-alkali stress resistance of apples. The product taking the brassinolide and the strigolactone as effective components is applied to plants stressed by saline-alkali, so that the saline-alkali stress resistance of the plants can be obviously enhanced, the oxidative damage of the saline-alkali stress to the plants is relieved, and the yield and quality reduction caused by the saline-alkali are relieved.)

1. A new application of an ester concentrated complexing agent containing brassinolide and strigolactone as effective components in improving saline-alkali stress resistance of apple is provided.

2. The new application of claim 1, characterized in that: the product containing brassinolide and strigolactone as effective components is a composite of brassinolide and strigolactone.

3. The new application of claim 2, characterized in that: the concentration of the brassinolide-monolaurate complex is 0.1 mM.

4. The new application of claim 3, characterized in that: the brassinolide and strigolactone compound is prepared by diluting 0.1M ethanol solution of brassinolide and strigolactone.

5. The new application of claim 1, characterized in that: the plant is a dicotyledonous plant.

6. The new application of claim 5, wherein: the dicotyledonous plant is Malus hupehensis Rehd.

7. The new use as claimed in any one of claims 1 to 6, wherein: the method for improving the saline-alkali stress resistance of the plants comprises the following steps: irrigating saline-alkali treatment liquid containing 0.1mM of brassinolide and strigolactone compound, and spraying 0.1mM of brassinolide and strigolactone compound.

8. The new application of claim 7, wherein: in the step, the irrigation frequency is 3 days and 1 time, and the total time is 5 times; the spraying frequency was 1 time for 1 day for a total of 15 times.

9. The new application of claim 8, wherein: the spraying amount of each plant is the same each time.

Technical Field

The invention relates to a new application of an ester concentrated complexing agent in improving saline-alkali stress resistance of apples.

Background

The stress to the plant during growth and development mainly includes two types, i.e., abiotic stress and biotic stress. In the abiotic stress suffered by plants in the growth and development process, the salinization of soil is not only a difficult problem in agricultural production in China, but also a well-known worldwide problem, which not only affects the normal production process of agriculture, but also hinders the high yield and stable production of agricultural products, and the saline-alkali soil contains more saline-alkali components, thus seriously affecting the quality of soil and the ecological process of the soil, and further affecting the growth of animals and plants. The saline-alkali soil area of China accounts for about 10% of the saline-alkali soil area of the world, the saline-alkali soil area is mainly distributed in northeast, northwest, north China and coastal areas, the saline-alkali soil area is too large, and the development of the agricultural industry of China is severely restricted.

The damage to plants by saline-alkali stress can be roughly divided into rapid osmotic stress and thereafter slow ionic toxicity. The former is mainly characterized in that the difficulty of water permeation and absorption caused by root system drought is similar to that of plant water potential reduction, physiological drought of plants is caused, and the diffusion resistance of CO2 is increased and the photosynthetic rate of leaves is limited in order to limit water consumption, reduce transpiration and close stomata of the plants. The ion toxicity is caused by Na in the salt⁺And Cl^-The toxicity caused by the large accumulation in the leaves can block the growth of new leaves of plants, reduce the accumulation of biomass, and cause oxidative damage and generation of active oxygen substances. A large amount of Na⁺Accumulate in photosynthetic cells, cause non-stomatal restriction, and reduce the photosynthetic capacity of plants. Therefore, the improvement of the saline-alkali stress resistance of the cultivated plants is imminent, which is of great significance in agricultural production.

The salt and alkali stress is different from the simple salt stress, and the alkaline salt in the salt is the main factor different from the salt and alkali stress. The damage of the saline-alkali stress to the plants has the characteristics of salt stress and also has the characteristics different from the salt stress, namely, the alkaline salt has high pH which is usually more than 8, and some soil even reaches more than 10, so the damage of the saline-alkali stress to the plants is far greater than the damage of the salt stress. In addition to osmotic stress and ionic toxicity, high pH stress is also a mechanism by which saline and alkaline stresses compromise plants. The influence of high pH stress on the roots of plants is huge, the roots of rice seedlings in alkaline solution can turn yellow immediately, the high pH around the roots of the plants can cause the accumulation and precipitation of metal ions and phosphorus, the tissue structure of the roots is damaged, the activity of the roots is reduced, the absorption function of the roots is weakened, the plants cannot normally absorb water and nutrition from the roots, the phenomena that the cells of the roots die and turn yellow, and then the leaves wither, the plants cannot normally carry out photosynthesis are shown, and therefore the normal physiological function is lost. Meanwhile, high pH can also cause nutrient elements in soil such as iron and phosphorus to be coagulated and agglomerated, so that the nutrient elements can not be normally absorbed by plants, and the growth of the plants is influenced.

Researches on the stress resistance of plants by using brassinolide and strigolactone as a class of antioxidants are increasing, but no one researches the influence of a composite solution of brassinolide and strigolactone on the saline-alkali stress of the plants at present. The invention adopts the brassinolide and strigolactone compound solution, reduces the damage of salt and alkali stress to plants, and obviously improves the capability of the plants for resisting the salt and alkali stress.

Disclosure of Invention

The technical problem to be solved by the invention is to overcome the defects of the prior art and provide a new application of an ester concentrated complexing agent in improving saline-alkali stress resistance of apples, which can remarkably enhance the saline-alkali stress resistance and reduce the oxidative damage of saline-alkali stress to plants.

In order to achieve the purpose, the invention adopts the following technical scheme:

new application of product containing brassinolide and strigolactone as effective components in improving saline-alkali stress resistance of plants is provided.

The product containing brassinolide and strigolactone as effective components is a composite of brassinolide and strigolactone.

The concentration of the brassinolide and strigolactone complex is 0.1 mM.

The brassinolide and strigolactone compound is prepared by diluting an ethanol solution of the brassinolide and strigolactone compound with distilled water.

The plant is dicotyledonous Malus hupehensis Rehd.

The method for improving the saline-alkali stress resistance of the plants comprises the following steps: the product with brassinolide and strigolactone as effective components is irrigated to the root and sprayed on the leaf surface of the plant seedling.

The frequency of irrigation was 3 days 1 time, for a total of 5 times; the spraying frequency was 1 time for 1 day for a total of 15 times.

The invention has the beneficial effects that: the brassinolide and strigolactone compound is applied to improving the saline-alkali stress resistance of plants, so that the saline-alkali stress resistance of the plants can be obviously improved, the oxidation damage to the plants caused by the saline-alkali stress is relieved, and the yield and quality reduction caused by the saline-alkali stress is relieved; in addition, it is convenient to apply and has good stability.

Detailed Description

The present invention will be further described with reference to the following examples.

Materials and reagents used in the following examples are all commercially available products unless otherwise specified.

The 0.1mM brassinolide and monolaurate complex used in the examples described below was diluted with 0.1M ethanol solution of brassinolide and monolaurate complex.

The products used in the examples below are brassinolide and monolaurate complexes.