Preparation for producing fermentation liquid fertilizer with livestock and poultry manure odor reducing effect and fermentation liquid fertilizer circulating device for reducing livestock and poultry
阅读说明:本技术 具有畜禽粪尿臭味减少效果的发酵液肥制造用制剂及畜禽粪尿臭味减少用发酵液肥循环装置 (Preparation for producing fermentation liquid fertilizer with livestock and poultry manure odor reducing effect and fermentation liquid fertilizer circulating device for reducing livestock and poultry) 是由 李相钟 李慧譞 金米姬 秋金星 李承协 于 2018-12-04 设计创作,主要内容包括:本发明提供具有畜禽粪尿臭味减少效果的发酵液肥制造用制剂及发酵液肥循环装置。本发明的发酵液肥制造用制剂的特征在于,包含由序列号1所表示的碱基序列构成且具有畜禽粪尿的臭味减少效果的新型微生物郭霍氏杆菌(Bacillus kochii)4584菌株(KCTC 13552BP)的菌体或其培养物。根据本发明,通过将硝化细菌有效微生物大量增殖而制造的发酵液肥持续循环至畜舍粪浆池,从而使畜舍粪浆池内有效微生物占优势,由此畜舍内所产生的粪尿的臭味被有效减少,同时有害微生物的增殖也被抑制,因此大幅改善畜舍饲育环境。(The invention provides a preparation for producing a fermentation liquid fertilizer and a fermentation liquid fertilizer circulating device, wherein the preparation has an effect of reducing the odor of livestock and poultry manure. The preparation for producing a fermented liquid fertilizer of the present invention is characterized by comprising a cell of a novel microorganism, strain Hoodia Guoshi (Bacillus kochii)4584 (KCTC13552BP), which is composed of the base sequence represented by SEQ ID NO. 1 and has an effect of reducing the odor of livestock and poultry feces and urine, or a culture thereof. According to the present invention, the fermented liquid manure produced by proliferating the nitrifying bacteria effective microorganisms in a large amount is continuously circulated to the livestock house manure pit, so that the effective microorganisms in the livestock house manure pit are dominant, whereby the odor of manure and urine produced in the livestock house is effectively reduced, and the proliferation of harmful microorganisms is also suppressed, thereby greatly improving the livestock house raising environment.)
1. A preparation for producing a fermented liquid fertilizer having an effect of reducing the odor of livestock and poultry feces and urine, which comprises a bacterial cell of a novel microorganism, Bacillus Guo-hicii (Bacillus kochii)4584 strain KCTC13552BP, or a culture thereof, which is composed of a base sequence represented by SEQ ID NO. 1 and has an effect of reducing the odor of livestock and poultry feces and urine.
2. The preparation for fermented liquid fertilizer production having an effect of reducing livestock manure odor according to claim 1, which contains a mixed strain further comprising any one or more of Bacillus kochii bacteriodans, Staphylococcus pasteurianus (Staphylococcus aureus), Bacillus velezensis (Bacillus velezensis), Geobacillus stearothermophilus, Geobacillus geophilus (Geobacillus georgillus) and Bacillus ginsengium as a nitrifying bacteria-effective microorganism, and a culture of the mixed strain as an effective ingredient.
3. The preparation for producing a fermented liquid fertilizer having an effect of reducing feces and urine odor of livestock and poultry according to claim 2, wherein the nitrifying bacteria are mixed with effective microorganisms, and further, any one or more of Lactobacillus casei (Lactobacillus casei), Bacillus methylotrophicus (Bacillus methylotrophicus), Lactobacillus chaff (Lactobacillus parafarraginis), Bacillus subtilis (Bacillus subtilis), Bacillus megaterium (Acetobacter lovaensis) and Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) are added.
4. The utility model provides a poultry excrement urine stink reduces with fertile circulating device of fermentation liquid which characterized in that includes:
a raw liquid tank (100) for mixing after feeding livestock manure transferred from a livestock house manure pit (10), the preparation for producing the fermentation liquid manure and chelated phyllite as a carbon source in an amount of 2-5 wt% based on the weight of the preparation,
An aerobic fermentation liquid manure tank (200) for producing an aerobic fermentation liquid manure by fermenting the livestock and poultry manure mixed with the preparation for producing a fermentation liquid manure supplied from the stock solution tank (100), and
a fermented liquid fertilizer supply pipe (300) for supplying the aerobic fermented liquid fertilizer in the aerobic fermented liquid fertilizing tank (200) to the livestock house manure pit (10),
the aerobic fermentation liquid manure supplied to the livestock house manure pit (10) is mixed with livestock manure and urine, and is supplied to the livestock house manure pit (10) through the raw liquid tank (100), the aerobic fermentation liquid manure tank (200), and the fermentation liquid manure supply pipe (300), thereby being repeatedly circulated.
5. The recycling apparatus of fermented liquid manure for reducing the odor of feces and urine of livestock and poultry according to claim 4, wherein,
the aerobic fermentation liquid fertilizing tank (200) comprises 4 fermentation tanks, namely a fermentation tank (220), a fermentation tank (230), a fermentation tank (240), a fermentation tank (250), a settling tank (260) and an activation liquid tank (270).
Technical Field
The present invention relates to a preparation for producing a fermented liquid manure having an effect of reducing the odor of livestock and poultry manure and a fermented liquid manure circulation device for reducing the odor of livestock and poultry manure using the same, and more particularly, to the following fermented liquid manure circulation device for reducing the odor of livestock and poultry manure: a preparation for producing a fermented liquid manure, which is produced by continuously feeding a preparation for producing a fermented liquid manure, which is produced by using, as an active ingredient, a nitrifying bacteria effective microorganism having an effect of reducing the odor of livestock and poultry manure, into a livestock house manure pit and circulating the same, so that the nitrifying bacteria effective microorganism remaining in the livestock house manure pit is dominant, thereby reducing the odor of the manure produced in the livestock house, and also, as the livestock and poultry manure is circulated in a storage amount of 120 days or more, not only is there no sludge decomposition effect and no proliferation of harmful microorganisms, but also the proliferation of the nitrifying bacteria effective microorganism can be carried out even if a disinfectant or an antibiotic which is generally used is used in a basic amount, thereby enabling the odor and the environment of the livestock and poultry manure to be improved more rapidly, and the fermented liquid manure is applied by a circulating breeding method, thereby industrially economically removing or reducing the odor and the environment of the livestock and poultry.
Background
Livestock environment maintenance organization livestock environment technical research institute has settled the odor problem and the coping technology of the most complaint livestock facilities recently because the odor problem is serious. Among complaints derived from livestock and poultry operations, complaints about odor account for about 60%, and odor countermeasures are problems to be solved urgently as one of environmental problems of livestock and poultry.
The difficulty of the odor countermeasure in livestock and poultry is that the odor generation source is extensive, the odor is not easy to be collected, and the odor is a compound odor, so that the technology capable of dealing with odor substances with different properties is required, according to the difference of the generation source, the small air volume can also be high-concentration odor, or the low-concentration air volume can also be high-air volume, and the concentration and the air volume can be obviously different due to the generation source.
In terms of the costs required for the odor countermeasures, if there is no help to the production of livestock and poultry but costs are required, it becomes a burden of business, and therefore the order of priority of the odor countermeasures is often left behind.
After the execution of the odor prevention law, a deodorizing technique for an odor generated in a fecal treatment facility has been developed in japan. Although the deodorizing apparatus according to the biological odor method using microorganisms is an effective technique as a solution for dealing with complaints, it is introduced only in a common composting center and a place where complaints need to be dealt with because of the cost of facilities.
On the other hand, livestock house ventilation odor has recently become a subject of complaints due to environmental changes around livestock houses, and low-concentration, high-air-volume odor countermeasures are required for livestock house environments and the like. Livestock houses and poultry houses in europe and the usa are mostly windowless livestock houses, and in recent years, technical development has been promoted due to the need for odor countermeasures.
As a related art, there is korean registered patent No. 10-1120095. The above patent discloses a method of using citrus tree sawdust and horse manure compost together with odor reducing microorganisms to pass or adsorb pig manure odor.
Further, korean laid-open patent No. 10-2012-0068194 discloses a method for deodorizing livestock manure by mixing a composition consisting of a mixture of bamboo leaves and pine leaves as forestry byproducts, indigenous microorganisms including Lactobacillus parakefir and Candida glabrata (Candida glabrata), and glucose anhydride in 10 to 30% by weight with the livestock manure fertilizer. However, in the case of the above patent, the treatment efficiency of artificially cultured microorganisms is greatly varied depending on the degree of putrefaction of feces and urine, and the financial resources used for reducing the odor of feces and urine of livestock and poultry are not easily available, so that it is not suitable for the large-scale treatment of feces and urine of livestock and poultry.
Further, Korean registered patent No. 10-0378667 discloses a method of composting by mixing a composition of odor-decomposing microorganisms with 5 to 10% of livestock manure and mixing sawdust, rice hulls, and fallen leaf powder with them and then aerobically fermenting them.
Further, Korean registered patent No. 10-0848677 discloses a method of mixing 30 to 50 parts by weight of leaf mold, 10 to 30 parts by weight of wood clay, 5 to 20 parts by weight of quicklime, 5 to 20 parts by weight of waste stone, and 0.5 to 2 parts by weight of thermophilic microorganisms with respect to 100 parts by weight of chicken manure and fermenting at a temperature of 50 to 60 ℃ for 5 to 7 weeks. However, in the case of the above patent, the amount of excipient to be charged is large relative to the amount of feces and urine to be treated, and therefore, the efficiency is extremely low, and the investment in mechanical equipment required for large-scale treatment is required, and therefore, it is not practical. Therefore, for effective treatment of livestock and poultry manure, development of a method for more rapidly and economically removing or reducing the odor of the livestock and poultry manure is required.
Disclosure of Invention
Problems to be solved
The present invention has an object to provide a preparation for producing a fermented liquid manure having an effect of reducing the odor of the manure generated in a livestock house and an effect of reducing the odor of the manure of livestock and poultry by circulating the manure of livestock and poultry for 120 days or more without causing the destruction of the decomposition effect of sludge and the proliferation of harmful microorganisms and allowing the proliferation of nitrifying bacteria effective microorganisms even when a disinfectant or an antibiotic which is generally used is used in a basic amount, thereby removing or reducing the odor of the manure of livestock and poultry more rapidly and economically and improving the environment, and a fermented liquid manure circulation device for reducing the odor of the manure of livestock and poultry using the preparation.
Means for solving the problems
The preparation for producing a fermented liquid fertilizer having an effect of reducing the odor of livestock and poultry manure according to the present invention for achieving the above object is characterized by comprising a cell of a novel microorganism, Bacillus kochii 4584 strain (KCTC13552BP), which is composed of the base sequence represented by sequence No. 1 and has an effect of reducing the odor of livestock and poultry manure, or a culture thereof.
The preparation is characterized in that the nitrifying bacteria effective microorganism further comprises a mixed strain containing at least one of Bacillus subtilis, Staphylococcus pasteurianus, Bacillus velezensis, Bacillus stearothermophilus and Bacillus georgillus, and a culture containing the mixed strain as an effective ingredient, more preferably, the nitrifying bacteria effective microorganism further comprises Lactobacillus casei, Bacillus methylotrophicus, Lactobacillus chaffeensis, Bacillus subtilis, Bacillus aceticus, Bacillus amyloliquefaciens, Bacillus subtilis, Bacillus aceticus, Bacillus amyloliquefaciens, and Bacillus amyloliquefaciens.
As another aspect of the present invention, a fermentation liquid manure circulation device for reducing the odor of livestock and poultry manure is characterized by comprising: a
The aerobic fermentation
The technical problem to be solved by the present invention is not limited to the above-mentioned technical problem, and other technical problems not mentioned can be clearly understood by those skilled in the art to which the present invention pertains from the following description.
Effects of the invention
According to the present invention, the fermented liquid manure produced by proliferating the nitrifying bacteria effective microorganisms in a large amount is continuously circulated to the livestock house manure pit, so that the effective microorganisms in the livestock house manure pit are dominant, whereby the odor of manure and urine produced in the livestock house is effectively reduced, and the proliferation of harmful microorganisms is also suppressed, thereby greatly improving the livestock house raising environment.
Further, since the liquid phase obtained by composting the livestock and poultry manure, which is stored for 120 to 150 days or more of the daily production amount of the manure, with the effective microorganism liquid is circulated through the pigsty and the fermentation tank, the odor of the livestock and poultry manure is rapidly and economically removed or reduced without additionally using a disinfectant or antibiotic, which is generally used, and the proliferation of harmful microorganisms is prevented.
Drawings
Fig. 1 is a view showing a fermented liquid manure recycling apparatus for reducing the odor of livestock and poultry manure using the fermented liquid manure preparation of example 1 of the present invention.
Description of the compliance
10: animal house excrement and milk pond
20: solid-liquid separator
30: livestock and poultry excrement and urine transport pipe
100: stock solution tank
200: aerobic fermentation liquid fertilizing tank
220. 230, 240, 250: fermentation tank
260: settling tank
270: activating liquid tank
280: microorganism culture device
300: fermentation liquid fertilizer supply pipe
400: circulation liquid storage tank
Detailed Description
Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. Detailed descriptions of well-known functions and constructions that may unnecessarily obscure the gist of the present invention will be omitted.
As shown in FIG. 1, the fermented liquid manure circulating apparatus for reducing the odor of livestock and poultry manure according to the present invention comprises a
In this case, the carbon source as an active ingredient of the preparation for producing a fermented liquid fertilizer is characterized in that 2 to 5 wt% of chelated phyllite is added to rapidly proliferate effective microorganisms. In addition, the odor of the livestock house is effectively reduced by adding the specific nitrobacteria compounded with the effective microorganisms, and particularly, the livestock manure is treated by the liquid phase circulation formed by composting the livestock manure with the effective microorganisms in 120-150 days of the daily production amount of the manure by the fermentation liquid manure circulation device for reducing the odor of the livestock manure, so that the environment in the livestock
In the present invention, the livestock manure treatment of 120 to 150 days or more of the amount of manure produced per day is disclosed, but the treatment is not limited thereto, and for example, if the amount of effective microorganisms added is increased to 2 times, 3 times, or 10 times or more, the livestock manure of as little as 15 days and as much as 150 days can be treated, and the livestock manure can be treated in a short time with a smaller amount of input.
Hereinafter, as an embodiment of the fermented liquid manure circulation device for reducing the odor of livestock and poultry manure according to the present invention, the main components included in the device and the active ingredients of the specific fermented liquid manure preparation used in the fermented liquid manure circulation device for reducing the odor of livestock and poultry manure are specifically as follows.
(main constituent element of fermentation liquid manure circulation device for reducing odor of livestock and poultry feces and urine)
1) A livestock
The livestock
The livestock manure collected in the livestock
2)
The preparation for producing a specific fermented liquid fertilizer transferred by the
In this case, the chelated phyllite is one produced by ultrafinely pulverizing crushed particles of phyllite (phylllite) to increase the absorption capacity of phyllite, and is an ore produced by hydrolysis, denaturation, weathering, corrosion, etc. of clay-based sedimentary layer, and is excellent in physical adsorption capacity and chemical cationic substitution, etc. since a large amount of mineral is properly mixed and a large amount of nano-sized minute spaces are formed inside the phyllite, the germanium (ge) content is 0.11ppm, and shows a value more excellent than the germanium content of ginseng or spring water, and thus the action of neutralizing or removing harmful substances is exerted by the physical adsorption capacity of phyllite and the chemical cationic substitution, etc.
The humic acid (humic acid) is a large polymer substance having a molecular weight of 10,000 to 100,000, which is solidified under an acidic condition and liquefied under an alkaline condition, and is known to have a function of improving digestion and absorption capacity, improving nutrient absorption, enhancing blood circulation, decomposing and chelating components of proteins and carbohydrates which are constituent molecules of pathogenic bacteria, viruses and physical substances, and excreting them to the outside of the body, and to have a function of preventing putrefaction and oxidation.
When the amount of the chelated phyllite or humic acid is less than 2 wt%, the growth of useful microorganisms is not easily achieved by addition, and when the amount is more than 5 wt%, the growth of useful microorganisms in the fermentation tank stages described below is rather decreased as the growth of useful microorganisms is rapidly increased, and the improvement effect by addition of additional substances may not be achieved.
The
The species of the effective microorganism of nitrifying bacteria is characterized by using a novel microorganism, Bacillus kochii 4584 strain (KCTC13552BP), which is composed of the base sequence represented by SEQ ID NO. 1 and has an effect of reducing the odor of livestock and poultry feces.
In other words, it was confirmed that the above-mentioned novel microorganisms exhibited excellent odor-reducing effects on livestock and poultry feces and urine, and the inventors of the present invention have deposited such a strain of Bacillus koehmannii at 20.06.months in 2018 at the Korean type culture Collection (KCTC) (accession No.: KCTC13552 BP).
Further, the nitrifying bacteria-effective microorganism may be a mixed strain obtained by further mixing more than one of Bacillus kochii bacteriohrurans, Staphylococcus pasteurii (Staphylococcus pasteurii), Bacillus velezensis (Bacillus velezensis), Geobacillus stearothermophilus and Geobacillus ginseng (Bacillus ceregerhuami) with the novel microorganism Bacillus kochii 4584 strain (KCTC13552BP), and more preferably, the mixed strain may further comprise more than one species selected from the group consisting of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), Lactobacillus casei (Lactobacillus casei), Bacillus methylotrophicus (Bacillus methylotrophicus), Lactobacillus chaffeensis (Lactobacillus paracasei), Bacillus subtilis (Bacillus subtilis) and Bacillus subtilis (Bacillus subtilis).
Although such a mixed strain of specific nitrifying bacteria effective microorganisms has been mentioned as an effect of promoting growth of crops, there is no precedent for using the mixed strain for reducing odor, and by constituting the mixed strain in the above-mentioned state, odor reducing ability higher than that of the strain used alone can be obtained, and the desired effect of the present invention can be obtained.
In the present specification, the culture means a substance containing all substances present in a medium in which a strain is cultured, and may mean, for example, a substance containing a metabolite or secretion which is a product of culture of the strain, or a pulverized product thereof, or the strain itself may be contained in the culture. Further, the above culture may be cultured according to a method generally used in the art.
The culture medium may be a normal culture medium, wherein the culture medium is prepared by culturing the nitrifying bacteria effective microorganism strains at 23 to 35 ℃ for 92 to 240 hours to prepare a culture of an effective microorganism mixture having a concentration of 1 × 10CFU/ml or more, more specifically, the culture medium may be inoculated with all of the nitrifying bacteria effective microorganism strains in an equal amount, and then cultured at 20 to 35 ℃ for 100 to 120 hours at an aeration rate of 0.05 to 0.4vvm (vol/vol/min), a stirring rate of 100 to 200rpm (rotation)/min, and an internal pressure of 0.1 to 1kgf/cm2, and the pH of the culture medium is preferably 6.0 to 9.0.
The preparation for producing a fermented liquid fertilizer thus produced may be constituted by using 100% by weight of the culture and further adding an excipient. In the case of the above excipient, a carbon source or a nitrogen source or a mixture thereof is used, and starch, amino acid, vitamin C, vitamin E, glucose, glycerol are preferably used alone or in combination of two or more thereof, but not limited thereto. The above-mentioned starch may be used for the purpose of preventing film formation caused by the sugar component. The starch may be used in an amount of 10 to 20 vol%, but is not limited thereto. When the amount of starch is increased, the microorganisms are diluted and the total number of bacteria is reduced. Vitamin C (ascorbic acid) can be used as a pH adjuster as well as vitamin use. The vitamin C may be used in an amount of 0.1 to 0.5 vol%, but is not limited thereto. The above vitamin C lowers pH when it is excessively input to possibly have an influence on the growth and activity of the strain. The glucose or glycerol may be used for stabilizing the microorganism.
The preparation for producing a fermented liquid fertilizer thus produced is stored in the
To explain, in the present invention, how much microbial activity can be achieved after the aerobic fermentation liquid manure is supplied to the
The preparation for producing a fermented liquid manure is mixed with the liquid-phase livestock and poultry manure in the
The livestock and poultry dung or urine may be dung or urine of mammals such as cow, pig, sheep and goat, and poultry such as pheasant, chicken, duck and turkey, but is not limited thereto.
3) Aerobic fermentation
The aerobic fermentation broth
As shown in fig. 1, an aerobic fermentation
In this case, the
The oxygen supply, that is, aeration, may be achieved by an air diffuser, and the air diffuser preferably reduces the capacity of a blower and power costs by providing a high-efficiency air diffuser having high oxygen transfer efficiency in the
In addition, in order to minimize the killing rate of the number of bacteria of the nitrifying bacteria effective microorganism mixture as an effective component of the preparation for producing a fermented liquid fertilizer of the present invention and to maximize the activity thereof so as to obtain the maximum odor reducing ability, the preparation for producing a fermented liquid fertilizer of the present invention has the following features: the reaction temperature of the
4) Fermentation liquid
The fermented liquid
As described above, the aerobic fermentation liquid manure produced by proliferating the nitrifying bacteria effective microorganisms in a large amount is continuously and cyclically supplied to the
Further, the total treatment capacity of the
The present invention will be described in more detail with reference to examples and experimental examples, which are provided for illustrative purposes only and do not limit the scope of the present invention.
<Example 1>The invention has the functions of the livestock and poultry manurePreparation of preparation 1 for producing fermented liquid fertilizer with reduced odor Make
A novel microorganism, Bacillus kochia 4584 strain (KCTC13552BP), which is a microorganism having a base sequence represented by SEQ ID NO. 1 and has an effect of reducing the odor of livestock manure, was prepared as a nitrifying bacteria-effective microorganism, and the microorganism was cultured at 30 ℃ for about 240 hours to prepare a culture having a concentration of 1X 10CFU/ml, thereby producing preparation 1 for producing a fermented liquid fertilizer of the present invention containing the microorganism as an active ingredient.
<Example 2>Preparation of preparation 2 for producing fermented liquid manure having effect of reducing odor of livestock and poultry feces and urine of the present invention Make
A preparation 2 for producing a fermented liquid fertilizer of the present invention, which contains a base sequence represented by SEQ ID NO. 1 and has an effect of reducing the odor of livestock and poultry manure, is produced by mixing together an equivalent amount of nitrifying bacteria effective microorganisms such as Bacillus guohii 4584 strain (KCTC13552BP), Bacillus kochiipsychrudorans, Staphylococcus pasteurii (Staphylococcus pasteurii), Bacillus belgii (Bacillus velezensis), Bacillus stearothermophilus (Geobacillus stearothermophilus) and Bacillus geophilus (Bacillus ginsengihumi), culturing the mixture at 30 ℃ for about 240 hours, and preparing a culture having a concentration of
<Example 3>Preparation 3 for producing a fermentation liquid fertilizer having an effect of reducing the odor of livestock and poultry feces and urine according to the present invention
In addition to the effective microorganisms of preparation 2 of example 2, Lactobacillus casei (Lactobacillus casei), Bacillus methylotrophicus (Bacillus methylotrophicus), Lactobacillus chaff (Lactobacillus parafarraginis), Bacillus subtilis (Bacillus subtilis), Acetobacter roxburghii (Acetobacter lovaniensis), and Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) were further added in equal amounts to produce preparation 3 for a fermented liquid fertilizer of the present invention.
<Example 4>Preparation 2 for producing fermented liquid fertilizer having effect of reducing odor of livestock and poultry manure Fermentation liquid fertilizer circulation for reducing livestock and poultry manure odorMethod of using a ring device
And respectively putting pig manure into 3 parts of the livestock
The pig manure contained in the 3-position of the livestock
The preparation for producing a fermented liquid fertilizer of example 3 contained in the
In the
The aerobic fermented liquid manure thus produced is transferred to a circulating
In this case, after the pig manure is treated for 120 days (about 3 months later), when the above-mentioned recycling process is completed, the aerobic fermentation liquid manure can be treated so that the solid-liquid separation process of pig manure does not occur, and thus the aerobic fermentation liquid manure can be used more economically.
<Comparative example 1>Preparation of preparation 1 for fermentation liquid fertilizer production
Preparation 1 for a fermented liquid fertilizer was prepared by the same method as in example 2 above, using as the applied strain an effective microorganism mixture consisting of only Lactobacillus casei (Lactobacillus casei), Bacillus methylotrophicus (Bacillus methylotrophicus), Lactobacillus chaff (Lactobacillus parafarraginis), Bacillus subtilis (Bacillus subtilis), Acetobacter lovans (Acetobacter lovaniensis), and Bacillus amyloliquefaciens (Bacillus amyloliquefaciens).
<Comparative example 2>Preparation for producing fermented liquid fertilizer 2
The mixed strain of the previously registered patent No. 10-1670651 is applied, and in detail, it is composed of Lactobacillus chaffeensis (Lactobacillus parafarraginis), Lactobacillus paracasei (Lactobacillus paracasei), Lactobacillus tolerans, Lactobacillus buehensis (Lactobacillus buchneri), Lactobacillus harbourneis (Lactobacillus harbinensis), Lactobacillus falciparum (Lactobacillus perolens), Lactobacillus rhamnosus (Lactobacillus rhamnosus), Lactobacillus bovis (Lactobacillus vacinus), Acetobacter rapae (Acetobacter lovaniensis), Acetobacter peroxidatus (Acetobacter peroxidans), Pichia pastoris (Pichia pastoris), Candida albicans (Bacillus subtilis), Lactobacillus acidophilus, Saccharomyces cerevisiae, Acetobacter oxysulfide (Weissense) and Bacillus subtilis), Bacillus subtilis7Preparation 2 for producing a fermentation liquid fertilizer containing an effective microorganism culture solution having a concentration of CFU/ml as an active ingredient.
<Experimental example 1>Confirmation of odor reducing ability of livestock and poultry feces and urine
1. Experimental methods
The preparation for producing fermented liquid manure of examples 1, 2 and 3 and the fermentation of comparative examples 1 and 2 were added to livestock manureThe liquid manure preparation was mixed with 20 wt% of the above livestock manure, fermented at 30 ℃ for 10 days, and hydrogen sulfide (H) was measured2S) gas concentration and ammonia (NH)3) The gas concentration.
At this time, the concentration of the test gas was measured by using a gas detection tube (old KS I2218: 2009).
2. Results of the experiment
The above experimental results show the results shown in table 1 below.
[ Table 1]
Hydrogen sulfide reduction ratio (%)
Ammonia reduction ratio (%)
Comparative example 1
70.1%
65.2%
Comparative example 2
75.1%
70.9%
Example 1
80.2%
80.0%
Example 2
85.2%
85.0%
Practice ofExample 3
89.5%
89.6%
As shown in table 1, it was confirmed that the preparations for producing fermentation liquid fertilizers of examples 1, 2 and 3 of the present invention exhibited high odor reducing ability in a short time even at a low bacterial count concentration.
<Experimental example 2>Confirmation of odor reducing ability in various stages of a fermenter
1. Experimental methods
The odor of pig manure was reduced by the method of using the fermentation liquid manure circulation device for reducing odor of livestock and poultry manure in example 4, and the activity of the preparation for producing a fermentation liquid manure of example 2 produced in each fermentation tank was confirmed (the number of bacteria of the mixed strain of effective microorganisms of each nitrifying bacterium was confirmed and ammonia (NH) was measured3) Gas concentration). In this case, as comparative examples, the preparation for producing a fermented liquid fertilizer of comparative example 1 (comparative example 3) was applied using the circulation device of example 4, or 1 or 3 fermentation tanks (comparative examples 4 and 5) were provided in the aerobic fermented
2. Results of the experiment
The above experimental results show the results shown in table 2 below.
[ Table 2]
As shown in table 2, it was confirmed that the number of bacteria in the strain of comparative example 3 increased through the aerobic fermentation liquid composting tank, but the ammonia reduction rate was not high relative to the increased number of bacteria, and in the case of comparative example 4 (using 1 fermentation tank) and comparative example 5 (using 3 fermentation tanks), the microbial activity between the fermentation tanks was maintained, but since the fermentation process was carried out for a long time in one fermentation tank, it was confirmed that harmful microorganisms (coliform bacteria, salmonella) were detected due to the re-production of harmful microorganisms produced in the pig manure, while in the case of example 4 of the present invention, it was confirmed that the harmful microorganisms were not detected, the odor reduction ability was excellent, and the strain was usable as a circulation device desired in the present invention.
In this way, by continuously circulating the fermented liquid manure produced by proliferating the nitrifying bacteria effective microorganisms in a large amount to the livestock house manure pit, it was confirmed that the effective microorganisms in the livestock house manure pit prevailed, whereby the odor of manure produced in the livestock house was effectively reduced, and the proliferation of harmful microorganisms was also suppressed, and the livestock house raising environment was greatly improved, and with the cyclic treatment of livestock manure for 120 days or more, it was found that there was no proliferation of harmful microorganisms, and the odor of livestock manure could be more quickly and economically removed or reduced even without additionally using a disinfectant or antibiotic that is generally used.
The present invention has been described above mainly in terms of preferred embodiments, and those skilled in the art to which the present invention pertains can realize embodiments different from the above detailed description of the present invention within the scope of the essential technology of the present invention. The essential technical scope of the present invention is presented here in the scope of claims, and it should be understood that all differences within the equivalent scope thereof are included in the present invention.
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