阅读说明：本技术 一种毛蜂窝菌菌油的提取方法及应用 (Extraction method and application of honeycomb fungus oil ) 是由 李皓 姚欣 朱峰 于 2019-10-25 设计创作，主要内容包括：本发明公开了一种毛蜂窝菌菌油的提取方法及应用,所述提取方法包括：取毛蜂窝菌子实体,干燥后粉碎,加入溶剂50℃下连续回流渗漉提取,收集提取液,50℃下水浴蒸馏回收溶剂后得粗制菌油；粗制菌油通过吸附层析柱,用洗脱剂洗脱,收集洗脱液,50℃下真空旋转蒸馏回收溶剂后得所述毛蜂窝菌菌油；所述方法操作简单,得油率高,且不会破坏其有效成分的结构,溶剂可回收循环使用。所述毛蜂窝菌菌油具有优良的抗氧化性能,无毒无害,且来源丰富,可用于制备抗氧化保健品。(The invention discloses an extraction method and application of a honeycomb fungus strain oil, wherein the extraction method comprises the following steps: drying and pulverizing fruiting body of pileus, adding solvent, extracting at 50 deg.C by continuous reflux percolation, collecting extractive solution, and distilling with water bath at 50 deg.C to recover solvent to obtain crude fungus oil; passing the crude oil through an adsorption chromatography column, eluting with an eluent, collecting the eluent, and recovering the solvent by vacuum rotary distillation at 50 ℃ to obtain the crude oil; the method has simple operation and high oil yield, does not damage the structure of the effective components, and can recycle the solvent. The crude honeycomb fungus oil has excellent oxidation resistance, is non-toxic and harmless, has rich sources, and can be used for preparing oxidation-resistant health-care products.)

1. A method for extracting the honeycomb fungus oil is characterized by comprising the following steps:

drying and pulverizing fruiting body of pileus, adding solvent, extracting at 50 deg.C by continuous reflux percolation, collecting extractive solution, and distilling with water bath at 50 deg.C to recover solvent to obtain crude fungus oil;

and (3) passing the crude oil through an adsorption chromatography column, eluting with an eluant, collecting eluent, and performing vacuum rotary distillation at 50 ℃ to recover the solvent to obtain the crude oil.

2. The method for extracting the trichogramma fungi oil according to claim 1, wherein the material ratio of the extraction process is 1: 20.

3. The extraction method of the trichogramma fungi oil as claimed in claim 1, wherein the extraction time is 20-25 h.

4. The method for extracting trichogramma fungi oil as claimed in claim 1, wherein the extracting solution comprises one or more of petroleum ether, diethyl ether, carbon disulfide, pentane, hexane, cyclohexane, acetone, chloroform and ethyl acetate.

5. The method for extracting trichogramma fungi oil as claimed in claim 1, wherein the eluent comprises one or more of petroleum ether, diethyl ether, carbon disulfide, pentane, hexane, cyclohexane and ethyl acetate.

6. The method of claim 1, wherein the adsorbent of the adsorption chromatography column comprises one of silica gel, activated carbon, or alumina.

7. The application of the honeycomb fungus oil is characterized in that the honeycomb fungus oil is used for preparing an antioxidant health-care product.

8. The use of the oil of claim 7, wherein the active ingredient of the oil comprises methyl linoleate, glycerol trioleate, methyl linoleate, cis-10-tetradecen-1-ol acetate, ethyl linoleate, diisooctyl phthalate, methyl palmitate, oleic acid, (Z, Z) -2-methyl-3, 13-octadecadienol, (Z) -methyl oleate, butyl hept-2-yl phthalate.

Technical Field

The invention relates to the field of extraction technology and application of fungus oil, in particular to an extraction method and application of a pileus went fungus oil.

Background

The trichoderma apiaria (Pers.) Fr., named Latin as Hexagonia apiaria (Pers.) and its alias longan comb, is a rare medicinal fungus belonging to Basidiomycota (Basidiomycota), Aphyllophorales (Aphyllophorales), Polyporaceae (Polyporaceae) and Hexagona (Hexagona). Widely distributed in tropical and subtropical regions, mainly grown on broad-leaved tree trunks. According to records of Chinese herbage, the chaetoceros is slightly bitter, astringent and warm, and has the effects of benefiting intestines, regulating qi to alleviate pain and invigorating stomach. Lihahua research and other researches find that the ethyl acetate extract of the honeycombed fimbria fermentation broth can obviously inhibit the activity of lung cancer cells NCI-H460, central nervous system cancer cells SF-268 and breast cancer cells MCF-7. However, no reports and products related to the pileus fungi oil are found yet.

It is seen that improvements and enhancements to the prior art are needed.

Disclosure of Invention

In view of the defects of the prior art, the invention aims to provide an extraction method and application of the chaetoceros oil, and aims to provide the extraction method of the chaetoceros oil and the application of the chaetoceros oil in the aspect of preparing health-care products.

In order to achieve the purpose, the invention adopts the following technical scheme:

a method for extracting the oil of the honeycomb fungus comprises the following steps:

drying and pulverizing fruiting body of pileus, adding solvent, extracting at 50 deg.C by continuous reflux percolation, collecting extractive solution, and distilling with water bath at 50 deg.C to recover solvent to obtain crude fungus oil;

and (3) passing the crude oil through an adsorption chromatography column, eluting with an eluant, collecting eluent, and performing vacuum rotary distillation at 50 ℃ to recover the solvent to obtain the crude oil.

In the method for extracting the trichogramma oil, the material ratio in the extraction process is 1: 20.

In the method for extracting the trichogramma oil, the extraction time is 20-25 h.

In the extraction method of the chaetoceros oil, the extracting solution comprises one or more of petroleum ether, diethyl ether, carbon disulfide, pentane, hexane, cyclohexane, acetone, chloroform and ethyl acetate.

In the extraction method of the chaetoceros oil, the eluent comprises one or more of petroleum ether, diethyl ether, carbon disulfide, pentane, hexane, cyclohexane and ethyl acetate.

In the method for extracting the crude honeycomb fungus oil, the adsorbent of the adsorption chromatography column comprises one of silica gel, activated carbon or alumina.

The oil of the chaetoceros is applied to preparing antioxidant health products.

In the application of the chaetoceros oil, the effective components of the chaetoceros oil comprise methyl linoleate, glycerol trioleate, methyl linoleate, cis-10-tetradecene-1-ol acetate, ethyl linoleate, diisooctyl phthalate, methyl palmitate, oleic acid, (Z, Z) -2-methyl-3, 13-octadecadienol, (Z) -methyl oleate and butyl hept-2-yl phthalate.

Has the advantages that:

the invention provides an extraction method and application of a crude honeycomb fungus oil, the crude honeycomb fungus oil is rich in wild resources, and can be artificially cultured, the fungus oil has effective antioxidant activity, the invention provides a method for effectively extracting the crude honeycomb fungus oil, the operation is simple, the implementation is easy, the extraction efficiency is high, and the used solvent can be recycled; the fungus oil extracted by the method has various effective components, does not destroy the activity of the fungus oil, and provides a good precondition for the application of the trichogramma fungus oil. The invention also provides the application of the fungus oil in the preparation of antioxidant health care products, and provides a good way for popularizing the application of the pileus fungus oil.

Drawings

FIG. 1 is a total ion flow diagram of GC-MS analysis of the oil of the trichogramma bacteria.

FIG. 2 is a graph showing the effect of the oil of the fungus Porphyra hirsuta on scavenging DPPH free radicals.

Detailed Description

The invention provides an extraction method and application of a pileus bacteria oil, and in order to make the purpose, technical scheme and effect of the invention clearer and clearer, the invention is further described in detail by referring to the attached drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

A method for extracting the oil of the honeycomb fungus comprises the following steps:

drying and crushing the sporocarp of the pileus, adding a solvent according to the material-liquid ratio of 1: 20, continuously refluxing and percolating at 50 ℃ for 20-25 h, collecting an extracting solution, and distilling in a water bath at 50 ℃ to recover the solvent to obtain crude fungus oil;

and (3) passing the crude oil through an adsorption chromatography column, eluting with an eluant, collecting eluent, and performing vacuum rotary distillation at 50 ℃ to recover the solvent to obtain the crude oil.

For the effective components of the pileus oil and the adopted solvent, the extraction and recovery of the solvent at 50 ℃ can not only accelerate the precipitation efficiency of the pileus oil and accelerate the volatilization of the solvent, but also can not damage the structure of the product. The extracted crude fungus oil contains impurities with large polarity, and the impurities with large polarity are adsorbed in the adsorption chromatographic column after adsorption and elution, so that the impurities can be effectively removed.

Specifically, the extract comprises one or more of petroleum ether, diethyl ether, carbon disulfide, pentane, hexane, cyclohexane, acetone, chloroform and ethyl acetate. The solution has good compatibility with the fungus oil, and has polarity close to that of effective components in the fungus oil.

In a preferred embodiment, the extract is diethyl ether. The boiling point of the ether is low, the polarity is small, most non-polar or low-polar lipid compounds are dissolved in the ether, and the compounds with larger polarity are difficult to dissolve in the ether; the extraction rate of the fungus oil is improved, and the polar compound impurities in the crude fungus oil are reduced; and the extracted extract is easy to recover.

The eluent comprises one or more of petroleum ether, diethyl ether, carbon disulfide, pentane, hexane, cyclohexane and ethyl acetate. The solvent has low polarity, and can elute low polarity oil compounds while retaining high polarity compounds in the adsorption chromatographic column.

In a preferred mode, the eluent is n-hexane-ethyl acetate with the volume ratio of 9: 1.

The adsorbent of the adsorption chromatographic column comprises one of silica gel, activated carbon or alumina, has good adsorption effect, and does not influence the effective components of the fungus oil.

The oil of the chaetoceros is applied to preparing antioxidant health products. The crude honeycomb fungus oil has excellent oxidation resistance, can effectively remove free radicals, has rich sources, and is suitable for being used as an effective component of an oxidation-resistant health-care product.

The dosage form of the health care product comprises one or more of tablets, sugar-coated pills, gelatin capsules, drinkable suspensions or disintegrable pastes; it can be administered orally or topically.

In the application of the chaetoceros oil, the effective components of the chaetoceros oil comprise methyl linoleate, glycerol trioleate, methyl linoleate, cis-10-tetradecene-1-ol acetate, ethyl linoleate, diisooctyl phthalate, methyl palmitate, oleic acid, (Z, Z) -2-methyl-3, 13-octadecadienol, (Z) -methyl oleate and butyl hept-2-yl phthalate.

Some examples are provided below to further illustrate the preparation of the oil of the chaetoceros and its antioxidant properties.