阅读说明：本技术 一种水酶法提取抗氧化芝麻油的方法 (Method for extracting antioxidant sesame oil by aqueous enzymatic method ) 是由 纪南军 于 2019-11-21 设计创作，主要内容包括：本发明公开一种水酶法提取抗氧化芝麻油的方法,采用水酶法对掺有葡萄籽的芝麻进行提取处理；本发明制备的一种水酶法提取抗氧化芝麻油的方法,提取的芝麻油具有优异的抗氧化性能,同时出油率高。(The invention discloses a method for extracting antioxidant sesame oil by an aqueous enzymatic method, which adopts the aqueous enzymatic method to extract and treat sesame doped with grape seeds; according to the method for extracting the antioxidant sesame oil by the aqueous enzymatic method, the extracted sesame oil has excellent antioxidant performance and high oil yield.)

1. A method for extracting antioxidant sesame oil by an aqueous enzymatic method is characterized in that the sesame oil doped with grape seeds is extracted by the aqueous enzymatic method;

the aqueous enzymatic method adopts compound enzyme for enzymolysis treatment;

the sesame doped with grape seeds is subjected to freezing and crushing treatment before the grape seeds are doped;

the freezing treatment of the sesame doped with grape seeds in the freezing and crushing treatment is to adopt a low-temperature calcium chloride solution to perform dipping treatment in a closed reaction kettle;

the dipping treatment is carried out in vacuum;

the vacuum degree of the vacuum is lower than 0.08 Mpa.

2. The method for extracting the antioxidant sesame oil by the aqueous enzymatic method as claimed in claim 1, wherein the extraction treatment is:

(1) screening complete and plump sesame, and drying until the water content is 8%;

(2) freezing and crushing the sesame obtained in the step (1), and sieving with a 60-mesh sieve to obtain sesame powder;

(3) crushing grape seeds, and sieving with a 100-mesh sieve to obtain grape seed powder;

(4) adding the grape seed powder obtained in the step (3) into the sesame powder obtained in the step (2), and uniformly stirring to obtain mixed powder, wherein the mixing mass ratio of the grape seed powder to the sesame powder is 1: 90-100;

(5) mixing the obtained mixed powder with distilled water according to a certain material-liquid ratio, uniformly stirring to obtain a mixed solution, adjusting the pH of the mixed solution to acidity, adding a complex enzyme with the mass of 1.5-2% into the mixed solution, heating to 36 ℃, stirring at the rotating speed of 60r/min for 3-4 hours, then carrying out steam enzyme deactivation treatment, and finally carrying out centrifugal separation to obtain the sesame oil.

3. The method for extracting the antioxidant sesame oil by the aqueous enzymatic method according to claim 1 or 2, wherein the complex enzyme is cellulase and acid protease, wherein the mixing mass ratio of the cellulase to the acid protease is 5-7: 3.

4. The method for extracting the antioxidant sesame oil by the aqueous enzymatic method according to claim 1 or 2, wherein the step of freeze-crushing is to perform a freezing treatment, the freezing treatment is to perform an immersion treatment in a closed reaction kettle by using a low-temperature calcium chloride solution, wherein the mass fraction of the calcium chloride solution is 17.8%, the freezing point of calcium chloride is-14.2 ℃, the initial temperature of the low-temperature calcium chloride solution is-10 ℃, and sesame and the low-temperature calcium chloride solution are mixed according to a ratio of 30 g: mixing 100mL of the mixture in a ratio, adding the mixture into a reaction kettle, soaking for 15min under vacuum, soaking by using a low-temperature calcium chloride solution, continuously reducing the temperature to-18 ℃, then soaking for 20min under vacuum, taking out, crushing, and crushing the sesame which is taken out.

5. The method for extracting antioxidative sesame oil by an aqueous enzymatic method according to claim 4, wherein the vacuum degree of the vacuum is 0.04 to 0.06 MPa.

6. The method for extracting the antioxidant sesame oil by the aqueous enzymatic method as claimed in claim 2, wherein the ratio of the materials to the liquids is 1: 7-9.

7. The method for extracting the antioxidant sesame oil by the aqueous enzymatic method as claimed in claim 2, wherein the pH adjustment is performed by using 0.1% by mass of hydrochloric acid solution.

8. The method for extracting the antioxidant sesame oil by the aqueous enzymatic method as claimed in claim 2, wherein the centrifugal speed of the centrifugal separation is 4000r/min, and the centrifugal separation time is 15 min.

Technical Field

The invention belongs to the technical field of sesame oil extraction, and particularly relates to a method for extracting antioxidant sesame oil by an aqueous enzymatic method.

Background

Sesame is one of main oil crops in China, the oil content of seeds of the sesame is high, the sesame oil is rich in fatty acid, linoleic acid and oleic acid which are necessary for human bodies, and the sesame oil has high application value. China is one of the main planting and producing countries of sesame, the yield of the sesame is the first world, and the sesame plays a very important role in daily life of people. Sesame has the reputation of "queen" oil crop, belongs to a large variety of oil in small variety of oil crop, and has extremely high nutritive value, health care function and medicinal value. Sesame oil is one of the three best edible oils published by the WHO, has high nutritional value and medicinal value, and is listed as the national drug dictionary of china, the united states, japan and the like, so the sesame oil is called as the king of oil. The sesame oil is rich in oleic acid and linoleic acid, and is very beneficial to softening blood vessels, reducing cholesterol and preventing atherosclerosis; also contains antioxidant substances such as vitamin E, sesamin, sesamol, etc., and has the function of remarkably delaying aging.

At present, the conventional sesame oil extraction methods mainly comprise a water extraction method, a mechanical squeezing method, a leaching method, an enzyme method and the like. Among them, the water substitution method is the most common traditional method for producing sesame oil, the mechanical squeezing method and the leaching method are the main methods for industrial production, and the enzyme method is a novel oil extraction method which is researched more in recent years. The water extraction method has the advantages of low extraction rate, low mechanization degree, high labor intensity, small workshop production as the main part, low automatic continuous degree, and high water content of the obtained sesame dregs, and is easy to decay, thereby reducing the storage life of the sesame oil. Although the squeezing method has simple process and is easy to realize industrial production, the oil yield is low, the residual oil content of cake residues is high, the impurity content is high, the process flow is long, the energy consumption is high, high temperature is formed inside the squeezing process, unsaturated fatty acid is easy to oxidize, the prepared crude oil has poor quality, the color is deep and easy to deteriorate, the oxidation resistance of the sesame oil is greatly reduced, the decay resistance of the sesame oil is further reduced, and the storage life of the sesame oil cannot be effectively prolonged.

Disclosure of Invention

The invention is realized by the following technical scheme.

A method for extracting antioxidant sesame oil by aqueous enzymatic method comprises extracting sesame oil doped with grape seed by aqueous enzymatic method;

the vacuum degree of the vacuum is lower than 0.08 Mpa.

Wherein the extraction process is as follows:

(1) screening complete and plump sesame, and drying until the water content is 8%;

(2) freezing and crushing the sesame obtained in the step (1), and sieving the sesame with a 60-mesh sieve to obtain sesame powder, wherein the freezing and crushing is to perform freezing treatment firstly, the freezing treatment is to perform dipping treatment in a closed reaction kettle by adopting a low-temperature calcium chloride solution, the mass fraction of the calcium chloride solution is 17.8%, the freezing point of calcium chloride is-14.2 ℃, the initial temperature of the low-temperature calcium chloride solution is-10 ℃, and the sesame and the low-temperature calcium chloride solution are mixed according to the proportion of 30 g: mixing 100mL of the mixture in a ratio, adding the mixture into a reaction kettle, soaking the mixture in vacuum for 15min, continuously reducing the temperature to 18 ℃ below zero after the mixture is soaked in a low-temperature calcium chloride solution, then soaking the mixture in vacuum for 20min, taking out the mixture, crushing the mixture, and crushing the sesame, wherein the vacuum degree of the vacuum is 0.04-0.06 MPa;

(3) crushing grape seeds, and sieving with a 100-mesh sieve to obtain grape seed powder;

(4) adding the grape seed powder obtained in the step (3) into the sesame powder obtained in the step (2), and uniformly stirring to obtain mixed powder, wherein the mixing mass ratio of the grape seed powder to the sesame powder is 1: 90-100;

(5) mixing the obtained mixed powder with distilled water according to the material-liquid ratio of 1:7-9, uniformly stirring to obtain a mixed solution, adjusting the pH of the mixed solution to acidity, wherein the pH is adjusted by adopting 0.1% hydrochloric acid solution, adding 1.5-2% of complex enzyme by mass into the mixed solution, the complex enzyme is cellulase and acid protease, the mass ratio of the cellulase to the acid protease is 5-7: 3, heating to 36 ℃, stirring for 3-4 hours at the rotating speed of 60r/min, then carrying out steam enzyme deactivation treatment, and finally carrying out centrifugal separation, wherein the centrifugal speed of the centrifugal separation is 4000r/min, and the centrifugal separation time is 15min to obtain the sesame oil.

According to the technical scheme, the beneficial effects of the invention are as follows:

according to the method for extracting the antioxidant sesame oil by the aqueous enzymatic method, the extracted sesame oil has excellent antioxidant performance and high oil yield; the sesame is crushed by adopting a freezing and crushing mode, the nutrition loss of the sesame can be reduced to a certain degree, the sesame is frozen by adopting a low-temperature calcium chloride solution, chloride ions, calcium ions and water molecules can permeate into oil material cells which can be degraded by using the sesame under the vacuum condition, then ice crystals can be generated by freezing, and grow gradually in the cell gaps inside the sesame through the ice crystals, so that the cells inside the sesame are extruded, the effect of internally destroying the cell structure is further played, particularly, the integrity of a grease body is damaged to a certain degree, the grease body is a complete, stable and small sphere which is independently existed in cytoplasm and is formed by a layer of Triacylglycerol (TAG) surrounded by a phospholipid membrane, and the protein is embedded on the phospholipid membrane, so that a complex enzyme can enter the phospholipid membrane for enzymolysis, and the tissue structure of the grease body can be further damaged, Hydrolyzing the macromolecular complex, and finally separating by utilizing the oil-water density difference; the sesame oil contains various unsaturated fatty acids, is easy to rancidity, oxidize and deteriorate, and the sesame oil extraction method has the advantages that a small amount of grape seeds are added to carry out synchronous extraction treatment at the initial extraction stage of the sesame oil, so that the grape seeds and the sesame oil can be uniformly combined together, chain reaction initiated by free radicals generated in the sesame oil can be greatly inhibited, the chain reaction is blocked by capturing the free radicals, and the effects of removing the free radicals and inhibiting lipid peroxidation can be achieved.

Drawings

FIG. 1 is a graph showing the effect of different feed liquid ratios on the oil yield of sesame oil.

Detailed Description