阅读说明：本技术 抗病促生复合芽孢杆菌水分散粒剂及其制备方法和应用 (Disease-resistant growth-promoting composite bacillus water dispersible granule as well as preparation method and application thereof ) 是由 王小兵 汪晓丽 封克 董君能 王海潮 麦帕雯 于 2019-10-12 设计创作，主要内容包括：本发明公开了一种抗病促生复合芽孢杆菌水分散粒剂,包括复合芽孢杆菌、载体和助剂,所述复合芽孢杆菌包括同温层芽孢杆菌SC-12和解淀粉芽孢杆菌BZ6-1,所述同温层芽孢杆菌SC-12和解淀粉芽孢杆菌BZ6-1的活菌数之比为2:1。本发明还公开了一种抗病促生复合芽孢杆菌水分散粒剂的制备方法,将混合培养的解淀粉芽孢杆菌BZ6-1和同温层芽孢杆菌SC-12混合培养的母液离心,将混合母液干燥后制成菌粉,在载体、湿润剂、分散剂、崩解剂、粘结剂中加入1%的菌粉,混合均匀后加水捏合,经过造粒、筛分、烘干得到产品。本发明抗病促生复合芽孢杆菌水分散粒剂可用于植物耐盐促生和生防抗病,提高植物产量和品质。(The invention discloses a disease-resistant growth-promoting composite bacillus water dispersible granule which comprises composite bacillus, a carrier and an auxiliary agent, wherein the composite bacillus comprises stratospheric bacillus SC-12 and bacillus amyloliquefaciens BZ6-1, and the ratio of the viable count of the stratospheric bacillus SC-12 to the viable count of the bacillus amyloliquefaciens BZ6-1 is 2: 1. The invention also discloses a preparation method of the disease-resistant growth-promoting composite bacillus water dispersible granule, which comprises the steps of centrifuging mother liquor obtained by mixed culture of bacillus amyloliquefaciens BZ6-1 and bacillus stratosphericus SC-12, drying the mixed mother liquor to prepare bacterial powder, adding 1% of bacterial powder into a carrier, a wetting agent, a dispersing agent, a disintegrating agent and a binder, uniformly mixing, adding water, kneading, granulating, screening and drying to obtain the product. The disease-resistant growth-promoting composite bacillus water dispersible granule can be used for salt-resistant growth promotion, biocontrol and disease resistance of plants, and can improve the yield and quality of the plants.)

1. The disease-resistant growth-promoting composite bacillus water dispersible granule is characterized by comprising composite bacillus, a carrier and an auxiliary agent, wherein the composite bacillus comprises bacillus pumilus SC-12 and bacillus amyloliquefaciens BZ6-1, and the ratio of the viable count of the bacillus pumilus SC-12 to the viable count of the bacillus amyloliquefaciens BZ6-1 is 2: 1.

2. The disease-resistant growth-promoting composite bacillus water dispersible granule as claimed in claim 1, wherein the auxiliary agent comprises a wetting agent, a dispersing agent, a disintegrating agent and a binder.

3. The disease-resistant growth-promoting composite bacillus water dispersible granule according to claim 1, wherein the carrier is one or more of talcum powder, calcium carbonate, ammonium sulfate and diatomite.

4. The disease-resistant growth-promoting composite bacillus water dispersible granule according to claim 2, wherein the content of the composite bacillus is 1%, the content of the wetting agent is 6%, the content of the dispersing agent is 6%, the content of the disintegrating agent is 4%, the content of the binder is 4%, and the balance of the carrier is up to 100%.

5. The disease-resistant growth-promoting composite bacillus water dispersible granule according to claim 2, wherein the wetting agent is one or more of sodium dodecyl sulfate, sodium dodecyl benzene sulfonate and saponin powder; the dispersing agent is one or more of sodium lignosulphonate, sodium carboxymethyl cellulose and sodium tripolyphosphate; the disintegrating agent is one or more of calcium chloride, ammonium sulfate and sodium chloride; the binder is one or more of polyvinyl alcohol, polyethylene glycol and soluble starch.

6. The disease-resistant growth-promoting composite bacillus water dispersible granule according to claim 2, wherein the carrier is diatomite, the wetting agent is sodium dodecyl sulfate, the dispersing agent is sodium carboxymethyl cellulose, the disintegrating agent is ammonium sulfate, and the binder is polyvinyl alcohol.

7. A preparation method of a disease-resistant growth-promoting composite bacillus water dispersible granule is characterized in that mother liquor obtained by mixed culture of bacillus amyloliquefaciens BZ6-1 and bacillus pumilus SC-12 is centrifuged, the mixed mother liquor is dried to prepare bacterial powder, 1% of the bacterial powder is added into a carrier, a wetting agent, a dispersing agent, a disintegrating agent and a binder, the bacterial powder is uniformly mixed and added with water to be kneaded, and the product is obtained through granulation, screening and drying.

8. The preparation method of the disease-resistant growth-promoting composite bacillus water dispersible granule according to claim 7, wherein the particle size of a screened sieve pore is 0.8mm, the drying temperature is 40 ℃, and the drying time is 45 min.

9. The disease-resistant growth-promoting composite bacillus water dispersible granule according to any one of claims 1-6 or the application of the disease-resistant growth-promoting composite bacillus water dispersible granule prepared by any one of claims 7-8 in salt tolerance growth promotion and biocontrol disease resistance of plants.

10. The application of the disease-resistant growth-promoting composite bacillus water dispersible granule according to any one of claims 1 to 6 or the disease-resistant growth-promoting composite bacillus water dispersible granule prepared by the method of any one of claims 7 to 8 in improving the yield and quality of plants.

Technical Field

The invention relates to a disease-resistant growth-promoting composite bacillus water dispersible granule as well as a preparation method and application thereof, belonging to the technical field of agricultural microorganisms.

Background

China is a big country of facility agriculture, farmers generally rely on big water to increase crop yield in order to pursue higher economic benefit, and secondary salinization of facility soil and serious occurrence of soil-borne diseases are caused. In recent years, researches on improving crop yield, preventing diseases and improving tolerance to various environments by utilizing rhizosphere salt-tolerant growth-promoting bacteria and biocontrol bacteria are one of the hot spots of researches on agricultural application microorganisms in recent years. The existing biological agent has single action and poor action effect and cannot meet the production requirement.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provide a disease-resistant growth-promoting composite bacillus water dispersible granule as well as a preparation method and application thereof, wherein a bacillus pumilus SC-12 strain and a bacillus amyloliquefaciens BZ6-1 strain are compositely cultured to construct a composite microbial inoculum to exert the efficacy of the composite microbial inoculum in a synergistic manner, and a multifunctional biological microbial inoculum with double effects of salt resistance, growth promotion, biological control and disease resistance is developed.

In order to solve the technical problems, the invention provides a disease-resistant growth-promoting composite bacillus water dispersible granule which comprises composite bacillus, a carrier and an auxiliary agent, wherein the composite bacillus comprises bacillus pumilus SC-12 and bacillus amyloliquefaciens BZ6-1, and the ratio of the viable count of the bacillus pumilus SC-12 to the viable count of the bacillus amyloliquefaciens BZ6-1 is 2: 1.

Bacillus pumilus SC-12(Bacillus pumilus) is preserved in the China general microbiological culture Collection center of China Committee for culture Collection of microorganisms, and the preservation address is as follows: west road No. 1 of north chen of chaoyang district, beijing, institute of microbiology of china academy of sciences, the date of deposit is No. 5 month 15 in 2013, the number of deposit is: CGMCC NO. 7622.

Bacillus amyloliquefaciens BZ6-1(Bacillus amyloliquefaciens) is deposited in a depositary designated by the national intellectual property office, wherein the deposition date is 2009, 01 and 22 days, and the name of the depositary is as follows: china general microbiological culture Collection center, preservation Address: the microbial research institute of China academy of sciences, university of Inward Yangtze district, Beijing, has a preservation number: CGMCC No. 2892.

Preferably, the auxiliaries include wetting agents, dispersants, disintegrants and binders.

Preferably, the carrier is one or more of talcum powder, calcium carbonate, ammonium sulfate and diatomite.

Preferably, the content of the bacillus compositus is 1%, the content of the wetting agent is 6%, the content of the dispersing agent is 6%, the content of the disintegrating agent is 4%, the content of the binder is 4%, and the balance of the carrier is 100%.

Preferably, the wetting agent is one or more of sodium dodecyl sulfate, sodium dodecyl benzene sulfonate and saponin powder; the dispersing agent is one or more of sodium lignosulphonate, sodium carboxymethyl cellulose and sodium tripolyphosphate; the disintegrating agent is one or more of calcium chloride, ammonium sulfate and sodium chloride; the binder is one or more of polyvinyl alcohol, polyethylene glycol and soluble starch.

Preferably, the carrier is diatomite, the wetting agent is sodium dodecyl sulfate, the dispersing agent is sodium carboxymethyl cellulose, the disintegrating agent is ammonium sulfate, and the binder is polyvinyl alcohol.

The invention also provides a preparation method of the disease-resistant growth-promoting composite bacillus water dispersible granule, which comprises the steps of centrifuging mother liquor obtained by mixed culture of bacillus amyloliquefaciens BZ6-1 and bacillus pumilus SC-12, drying the mixed mother liquor to prepare bacterial powder, adding 1% of bacterial powder into a carrier, a wetting agent, a dispersing agent, a disintegrating agent and a binder, uniformly mixing, adding water, kneading, granulating, screening and drying to obtain the product.

Preferably, the particle size of the screened mesh is 0.8mm, the drying temperature is 40 ℃, and the drying time is 45 min.

The invention also provides application of the disease-resistant growth-promoting composite bacillus water dispersible granule in salt-tolerant growth promotion, biocontrol and disease resistance of plants.

The invention also provides application of the disease-resistant growth-promoting composite bacillus water dispersible granule in improving the yield and quality of plants.

The invention achieves the following beneficial effects:

(1) the ACC deaminase specific activity of the Bacillus pumilus SC-12 strain (Bacillus pumilus) reaches 0.087U/mg, and the Bacillus pumilus SC-12 strain has strong siderophore capacity, and can remarkably promote the germination rate and the growth of buds of plants under the stress conditions of 100mmol/L nitrate and 200 mmol/L nitrate; the bacillus amyloliquefaciens BZ6-1 strain (Bacillus amyloliquefaciens) has stronger inhibiting effect on bacterial diseases such as ralstonia solanacearum and the like and fungal diseases such as rice sheath blight bacteria and rice blast bacteria and the like, and has wider antibacterial spectrum; the invention compositely cultures the Bacillus pumilus SC-12 strain and the Bacillus amyloliquefaciens BZ6-1 strain to construct a composite microbial inoculum to cooperatively exert the efficacy of the composite microbial inoculum and develop a multifunctional biological microbial inoculum which achieves the double effects of salt tolerance, growth promotion, biocontrol and disease resistance.

(2) When the water dispersible granule is used, the water dispersible granule is put into water to be rapidly disintegrated and dispersed to form a high-suspension system, and has the advantages of high dispersibility, high effectiveness, high stability and environmental friendliness.

(3) The addition of the auxiliary agent can enhance the control effect of the water dispersible granule, improve the physicochemical property and the service performance of the water dispersible granule, reduce the dosage of the water dispersible granule, save the cost and protect the environment.

(4) The spore content of the compound water dispersible granule prepared by the invention is 2.5 multiplied by 10⁸cfu·g^-1The suspension percentage is 79.3%, the pH value is 6.8, the water content is 4.5%, the wetting time is 19.6s, the disintegration time is 86.4s, the granule strength is moderate, and the application of the water dispersible granule improves the yield and the quality of plants.

Drawings

FIG. 1 is a process flow chart of the disease-resistant growth-promoting composite bacillus water dispersible granule of the invention;

FIG. 2 is a graph showing the effect of different storage modes on the activity of bacillus water dispersible granules.

Detailed Description

The invention is further described below with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and the protection scope of the present invention is not limited thereby.