阅读说明：本技术 一种液相芯片工作站 (Liquid phase chip workstation ) 是由 车团结 徐红 李春 沈颂东 张莹 李潇玲 杨涛 于 2019-08-09 设计创作，主要内容包括：本发明涉及生物学分析检测技术领域,具体涉及一种液相芯片工作站,主要解决的技术问题在于克服现有技术中的检测效率的缺陷,其技术要点在于：包括液相芯片,包括聚合物微球、标识分子和探针分子；反应模块,包括激发光源、微流通道和光学板块,所述激发光源折射入所述微流通道；分析模块,包括计算机硬件和图像分析软件,接收并处理来自反应模块的数据,反应板块的存在能够将微球多排、连续、均匀、平稳、匀速地通过宽微流通道系统,使检测速度和效率得到显著的提高,每秒可检测几千个微球,简化了需要人工设定的步骤,显著的提高了检测的效率。(The invention relates to the technical field of biological analysis and detection, in particular to a liquid-phase chip workstation, which mainly solves the technical problem of overcoming the defect of detection efficiency in the prior art and has the technical key points that: comprises a liquid phase chip which comprises polymer microspheres, marking molecules and probe molecules; the reaction module comprises an excitation light source, a microfluidic channel and an optical plate, wherein the excitation light source is refracted into the microfluidic channel; the analysis module comprises computer hardware and image analysis software, receives and processes data from the reaction module, the existence of the reaction plate can enable the microspheres to pass through the wide microchannel system in a multi-row, continuous, uniform, stable and uniform mode, so that the detection speed and efficiency are obviously improved, thousands of microspheres can be detected per second, the steps needing manual setting are simplified, and the detection efficiency is obviously improved.)

1. A liquid phase chip workstation, comprising:

the liquid phase chip (1) comprises polymer microspheres (11), identification molecules (12) and probe molecules (13);

the reaction module (2) comprises an excitation light source, a microfluidic channel (22) and an optical plate (23), wherein the excitation light source refracts into the microfluidic channel (22);

an analysis module (3), comprising computer hardware (31) and image analysis software (32), receives and processes data from the reaction module (2).

2. The liquid phase chip (1) workstation according to claim 1, wherein the liquid phase chip (1) is mixed and added into the sample solution to be tested, and the marker molecule (12) and the probe molecule (13) are independently dispensed or premixed.

3. The liquid chip workstation as claimed in claim 2, characterized in that the identification molecule (12) is a fluorescent protein with one or more spectral properties.

4. The liquid phase chip workstation as claimed in claim 3, wherein the microfluidic channel (22) is controlled by a stepping motor (221), and a micro vortex oscillator (222) is fixed below the microfluidic channel (22) through a bracket.

5. The LCP workstation according to claim 4, wherein said excitation light source is a two-way laser, and said laser light sources (21) are coaxial.

6. The liquid phase chip (1) workstation as claimed in claim 5, wherein the optical plate (23) comprises a collecting mirror (231) and a blocking filter (232), the collecting mirror (231) is used for collecting the excitation fluorescence emitted from the microfluidic channel (22), and the blocking filter (232) is used for blocking the reflected light of the excitation light source.

7. The liquid phase chip workstation according to claim 6, wherein said optical plate (23) further comprises a filtered shutter (233) and a detector (234), said filtered shutter (233) being controlled by said computer hardware (31) to have a speed at least four times faster than the flow rate in the microsphere channel, said detector (234) being in communication with said computer hardware (31), said detector (234) and said computer hardware (31) performing mutual checking of the working status by the interaction of handshake signals.

8. The liquid phase chip workstation as claimed in claim 7, wherein said computer hardware (31) is capable of acquiring image data from said detector (234), said image analysis software (32) comprises a detection data statistical method and a data result judgment standard, and said computer hardware (31) and said image analysis software (32) perform mutual checking of working states through interaction of heartbeat signals.

Technical Field

The invention relates to the technical field of biological analysis and detection, in particular to a liquid-phase chip workstation.

Background

With the rapid development of analytical diagnosis technology, the liquid-phase chip which is known as the later gene era and developed in the middle of the 90 th century is a novel protein research platform which combines the flow cytometry detection technology and the traditional chip technology. The system organically integrates colored microspheres, modern immunity technology, laser technology, applied fluidics, latest high-speed digital signal processor and computer algorithm, and achieves extremely high detection specificity and sensitivity. Can be used for clinical disease diagnosis, such as cytokine, allergen and autoimmune reaction detection, HLA typing, SNP detection, tumor specific antigen quantitative detection, multiple microorganism quantitative detection, etc.; or in basic research applications, such as: genotyping, protein expression typing, enzyme-substrate analysis, nucleic acid research, etc.; the method can also be applied to the aspects of food safety, multiple quantitative detection of pesticide and veterinary drug residues, judicial identification and the like.

The liquid phase chip detector on the market all adopts the same or similar principle, and its principle is: the method is characterized in that a plurality of round microspheres (usually 5.6um in diameter) with uniform size are used as main substrates, the microspheres are coded by two organic fluorescent dyes, different probe molecules are fixed on each microsphere, the microspheres are suspended in a liquid phase system to form a liquid phase chip, an object to be detected and a report fluorescent molecule are added to carry out immunoreaction or nucleic acid hybridization reaction, the coded fluorescence and the report fluorescence on the microspheres are excited by two beams of laser, and the species and the number of the detected species are obtained through the calculation of a laser reader and a computer.

Due to the limitation of the fluorescence spectral bandwidth and the signal intensity detection dynamic range, when the total number of required codes is large, the group number differentiation adopted by each level of codes is usually small, so that the detection performance index of an instrument is more dependent, the optical system is complex, the size is large, the cost is high, and the detection speed is also limited, so that a liquid-phase chip workstation is required to solve the defects.

Disclosure of Invention

Therefore, the technical problem to be solved by the present invention is to overcome the defect of detection efficiency in the prior art, and to provide a liquid phase chip workstation.

The technical purpose of the invention is realized by the following technical scheme:

a liquid phase chip workstation comprising:

the liquid phase chip comprises polymer microspheres, a marker molecule and a probe molecule;

the reaction module comprises an excitation light source, a microfluidic channel and an optical plate, wherein the excitation light source is refracted into the microfluidic channel;

an analysis module, including computer hardware and image analysis software, receives and processes data from the reaction module.

Further, the liquid phase chip is mixed and added into the sample solution to be detected, and the identification molecule and the probe molecule are independently subpackaged or premixed.

Further, the marker molecule is a fluorescent protein having one or more spectral characteristics.

Furthermore, the micro-flow channel is controlled by a stepping motor, and a micro vortex oscillator is fixed below the micro-flow channel through a support.

Furthermore, the excitation light source is two lasers, and the laser light sources share an optical axis.

Furthermore, the optical plate comprises a collecting lens and a blocking filter plate, wherein the collecting lens is used for collecting the excitation fluorescence emitted from the microfluidic channel, and the blocking filter plate is used for blocking the reflected light of the excitation light source.

Furthermore, the optical plate also comprises a filter shutter and a detector, the speed of the filter shutter is controlled by the computer hardware to be at least four times faster than the flow speed in the microsphere channel, the detector is communicated with the computer hardware, and the detector and the computer hardware carry out mutual check of working states through the interaction of handshake signals.

Further, the computer hardware can acquire image data from the detector, the image analysis software comprises a detection data statistical method and a data result judgment standard, and the computer hardware and the image analysis software perform mutual check of working states through interaction of heartbeat signals.

The technical scheme of the invention has the following advantages:

1. according to the liquid-phase chip workstation provided by the invention, the marker molecules and the probe molecules have the advantages of stable fluorescence, strong fluorescence intensity, difficulty in photobleaching and the like, so that the stability, the sensitivity and the resolution are higher, the vortex oscillation structure can be added to uniformly mix the marker molecules and the probe molecules, and the uniformity of the uniform mixing effect is ensured.

2. According to the liquid-phase chip workstation provided by the invention, the micro-flow channel can enable microspheres to pass through the wide micro-flow channel system in a multi-row, continuous, uniform, stable and uniform manner, so that the detection speed and efficiency are obviously improved, thousands of microspheres can be detected per second, the steps needing manual setting are simplified, and the detection efficiency is obviously improved.

3. The liquid phase chip workstation provided by the invention can be used for shooting the fluorescence with different wavelengths through the detector in the optical plate, distinguishing and processing the image through image analysis software, sending the image to a computer for processing and analyzing the signal, and finally outputting an analysis report. Thus making the entire structure simple and compact.

4. The liquid-phase chip workstation provided by the invention can be used for mutually checking the working states through the interaction of the handshake signals, so that the stability of the system is improved; mutual check of working states is carried out through interaction of heartbeat signals, and information loss is effectively prevented.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a schematic diagram of a liquid phase chip workstation according to an embodiment of the present invention;

FIG. 2 is a block diagram of a liquid phase chip workstation according to an embodiment of the present invention;

fig. 3 is a schematic structural diagram of an optical plate of a liquid phase chip workstation according to an embodiment of the present invention.

Description of reference numerals:

1. a liquid phase chip; 11. polymeric microspheres; 12. a marker molecule; 13. a probe molecule; 2. a reaction module; 21. a laser light source; 22. a microfluidic channel; 221. a stepping motor; 222. a micro vortex oscillator; 23. an optical plate; 231. a condenser lens; 232. blocking the filter plate; 233. a light filtering gate; 234. a detector; 3. an analysis module; 31. computer hardware; 32. image analysis software.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

A liquid phase chip workstation, as shown in figure 1, the liquid phase chip 1 obtains the result through the analysis module 3 through the reaction module 2 finally, the reaction module 2 includes the excitation light source, microflow passage 22 and optical plate 23, the excitation light source refracts into the microflow passage 22, the microflow passage 22 is controlled through the step motor 221, the microflow passage 22 below is fixed with the miniature vortex oscillator 222 through the support, the microflow passage 22 can pass through the wide microflow passage 22 system with the microballon multirow, continuously, evenly, steadily, at the uniform velocity, the vortex oscillation structure that adds can mix it evenly, guarantee simultaneously that the mixing effect is unanimous, make detection speed and efficiency obtain the obvious improvement, can detect thousands of microballons per second, the step that needs artifical settlement has been simplified, the apparent efficiency that has improved the detection.

As shown in fig. 1 and 2, the liquid chip 1 is mixed and added into the sample solution to be tested, the marker molecules 12 and the probe molecules 13 are independently packaged or premixed, the marker molecules 12 have one or more kinds of fluorescent proteins with spectral characteristics, and the marker molecules 12 and the probe molecules 13 have the advantages of stable fluorescence, strong fluorescence intensity, difficulty in photobleaching and the like, so that the stability, the sensitivity and the resolution are higher.

As shown in fig. 2, the detector 234 is communicated with the computer hardware, the computer hardware 31 can acquire image data from the detector 234, the detector 234 is communicated with the computer hardware, the detector 234 and the computer hardware 31 perform mutual check of working states through interaction of handshake signals, the computer hardware 31 can acquire image data from the detector 234, the image analysis software 32 includes a detection data statistical method and a data result judgment standard, the computer hardware 31 and the image analysis software 32 perform mutual check of working states through interaction of heartbeat signals, and perform mutual check of working states through interaction of handshake signals, thereby improving stability of the system; mutual check of working states is carried out through interaction of heartbeat signals, and information loss is effectively prevented.

As shown in fig. 3, the excitation light source is two lasers, the laser light source 21 is coaxial, the optical plate 23 includes a collecting lens 231 and a blocking filter 232, the collecting lens 231 is used for collecting the excitation fluorescence emitted from the microfluidic channel 22, the blocking filter 232 is used for blocking the reflected light of the excitation light source, the optical plate 23 further includes a filter gate 233 and a detector 234, the speed of the filter gate 233 is controlled by the computer hardware 31 to be at least four times faster than the flow speed in the microsphere channel, the fluorescence with different wavelengths is captured by the detector 234 in the optical plate 23, the image is resolved and processed by the image analysis software 32, and then the image is handed to the computer for processing and analyzing the signal, and finally an analysis report is given. Thereby making the whole structure simple and compact

The working principle of the liquid phase chip workstation is as follows: the liquid phase chip 1 is premixed and added into the liquid to be detected, the fluid flows through the microfluidic channel 22, the stepping motor 221 and the micro vortex oscillator 222 work, the reaction is carried out in the optical plate 23, and the obtained signal data is transmitted to the analysis module 3. It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.