阅读说明：本技术 一种清除杉虎斑寄生鱼蛭的方法 (Method for removing parasitic leech of fir tiger spot ) 是由 熊向英 蔡昭进 赵艳飞 刘旭佳 姚坤志 王志成 于 2019-09-03 设计创作，主要内容包括：本发明公开了一种清除杉虎斑寄生鱼蛭的方法,主要包括下述步骤：向养殖有总体质量为240-250kg杉虎斑的面积为15m<Sup>2</Sup>,水深1m水泥池中加入硫酸铜硫酸亚铁粉40-50g,浸泡18-20分钟；加入阿维菌素10-20ml浸泡50-60分钟；全部换水后加入多元有机酸类解毒剂7-11g解毒；等待6-7天,重复以上(1)-(3)步骤。本发明利用硫酸铜硫酸亚铁粉中的Cu<Sup>2+</Sup>可与蛋白质结合形成螯合物,使蛋白质变性、沉淀,从而使杉虎斑鱼身上的寄生鱼蛭体内酶失去活性,同时硫酸亚铁具有收敛作用,辅助杀虫,阿维菌素则通过干扰鱼蛭神经生理活动,刺激释放Y-氨基丁酸,鱼蛭与阿维菌素接触后会出现麻痹症状,不活动不取食而死亡。残余虫卵未发育成性成熟能够产卵的成虫时重复一遍即能解决鱼蛭病反复暴发的问题。(The invention discloses a method for removing Chinese fir tiger spot parasitic leech, which mainly comprises the following steps: the area of the China fir tiger spot with the total mass of 240- 2 Adding 40-50g of copper sulfate ferrous sulfate powder into a cement pool with the water depth of 1m, and soaking for 18-20 minutes; adding 10-20ml of abamectin and soaking for 50-60 minutes; adding 7-11g of polybasic organic acid antidote after all water is changed for detoxification; waiting for 6-7 days, and repeating the steps (1) - (3). The invention utilizes Cu in the copper sulfate ferrous sulfate powder 2+ Can be combined with protein to form chelate, so that the protein is denatured and precipitated, so that the enzyme in the parasitic leech on the tiger fish body is inactivated, simultaneously, the ferrous sulfate has the function of convergence and assists in killing insects, the abamectin stimulates to release Y-aminobutyric acid by interfering the nerve physiological activity of the leech, and the leech can generate paralysis after contacting with the abamectin and die without eating. The problem of repeated outbreak of the leech disease can be solved by repeating the process once when the residual worm eggs are not developed into sexually mature adults capable of laying eggs.)

1. A method for removing parasitic leech of fir tiger spot is characterized by mainly comprising the following steps:

(1) the tiger spot parasitizing the leech is 250kg/15m³The density of the culture medium is 15m²Draining water in a cement pond with the water depth of 1m to the water depth of 30cm, adding 40-50g of copper sulfate ferrous sulfate powder, and soaking for 18-20 minutes;

(2) adding 10-20ml of abamectin with the concentration of 1% and soaking for 50-60 minutes;

(3) adding 7-11g of polybasic organic acid antidote after all water is changed for detoxification;

(4) and (3) waiting for 6-7 days, and repeating the steps (1) to (3) to obtain the product.

2. The method for removing the leech parasitic on the fir tiger spot as claimed in claim 1, which comprises the following steps: the soaking time of the copper sulfate ferrous sulfate powder is 20 minutes.

3. The method for removing the leech parasitic on the fir tiger spot as claimed in claim 1, which comprises the following steps: the soaking time of the abamectin is 60 minutes.

Technical Field

The invention relates to a method for removing Chinese fir tiger spot parasitic leech, belonging to the field of aquaculture.

Background

The fir tiger spot is a hybrid of a male fir spot and a female tiger spot, has stronger environmental adaptability, transportation resistance and delicious meat quality compared with the tiger spot, enters the market as a high-end fish and has considerable profit.

The leech is commonly called seawater leech, ground beetle, black beetle and the like, is an ectoparasite in grouper culture, is mainly parasitic on the pectoral fin and the base part of the pectoral fin of the grouper body, is parasitic on the head, the dorsal fin and the tail fin of the fish body, and is also parasitic on the eyes of the grouper. The leech has high propagation speed, can cause the cultured grouper to be rotten, thin, malnutrition and serious yield reduction, is especially used as a vector for disease transmission such as blood bacteria, viruses, parasites and the like to cause large-scale morbidity of the cultured fish, and causes great loss to the large-scale culture of the fir tiger spots.

The leech is adsorbed on the body surface of tiger spot and can be seen by naked eyes, the length of the leech is about 0.9-1.5cm, the leech is black brown, has a link and can stretch out and draw back, the front sucker is small and is used for being adsorbed on a host to suck host blood, the rear sucker is large and is used for adsorbing the host to assist the front sucker to crawl or crawl on the body surface of a diseased fish, and the leech is good in flexibility and not easy to pull out.

Disclosure of Invention

The invention aims to solve the problem of providing a method for removing Chinese fir tiger spot parasitic leeches, and overcomes the harm that Chinese fir tiger spots are easy to be parasitic by leeches and relapse in the culture process, so that the large-scale disease attack and even the yield reduction of the Chinese fir tiger spots are caused.

In order to solve the problems, the following technical scheme is adopted, and the method for removing the parasitic leech of the fir tiger spot mainly comprises the following steps:

(1) the tiger spot parasitizing the leech is 250kg/15m³The density of the culture medium is 15m²Draining water in a cement pond with the water depth of 1m to the water depth of 30cm, adding 40-50g of copper sulfate ferrous sulfate powder, and soaking for 18-20 minutes;

(2) adding 10-20ml of abamectin with the concentration of 1% and soaking for 50-60 minutes;

(3) adding 7-11g of polybasic organic acid antidote after all water is changed for detoxification;

(4) and (3) waiting for 6-7 days, and repeating the steps (1) to (3) to obtain the product.

Preferably, the soaking time of the copper sulfate ferrous sulfate powder is 20 minutes.

Preferably, the soaking time of the abamectin is 60 minutes.

The invention utilizes Cu in the copper sulfate ferrous sulfate powder²⁺Can be combined with protein to form chelate, so that the protein is denatured and precipitated, and the enzyme in the parasitic leech on the tiger fish body is inactivated, thereby playing the role of killing parasites, and simultaneously, the ferrous sulfate has the convergence function and plays the role of assisting in killing insects. The abamectin stimulates the release of Y-aminobutyric acid by interfering the neurophysiological activity of the leech, and the leech can generate paralysis after contacting with the abamectin and die without eating. The polybasic organic acid can quickly complex or degrade harmful substances in water such as ammonia, nitrous acid, organic acid, heavy metal and the like, is used for degrading residual copper sulfate ferrous sulfate powder in water, and plays a role in detoxifying. Meanwhile, the medicament has no effect on the leech eggs, so the leech disease is easy to relapse. Therefore, the residual leech eggs in the cement pond develop into imagoes visible to the naked eye, the steps of killing insects are repeated once when sexual maturity is not reached and eggs can lay, and the problem of repeated relapse of leech diseases can be well solved.

The method of the invention can achieve the purpose of killing the leech parasitized by fir tiger spots and can better solve the problem of repeated outbreak of the leech disease. Practice and detection prove that after the method is used, all the leeches parasitizing on the fir tiger spots die of the disease, and the fir tiger spots have no adverse reaction.

Detailed Description

The technical solution of the present invention will be further described below by way of examples.