阅读说明：本技术 一种壳聚糖纳米颗粒乳牙根管充填材料及其制备方法 (Chitosan nano-particle deciduous tooth root canal filling material and preparation method thereof ) 是由 沈仁泽 叶展超 柯淑女 郭丽丽 李延鑫 王雪 童泽榕 姜海敏 苏美旋 黄振贤 于 2019-10-31 设计创作，主要内容包括：一种壳聚糖纳米颗粒乳牙根管充填材料及其制备方法,属于医用材料领域。壳聚糖纳米颗粒乳牙根管充填材料的组分按质量百分比为壳聚糖99％～50％,所载药物1％～50％,所述壳聚糖为主体支架材料,所述所载药物为调节材料降解速率及增加治疗效果的调节材料。在冰醋酸溶液加入壳聚糖,搅拌溶解,利用碱性液体调节pH值,将壳聚糖溶液置于磁力搅拌器上,750～1000r/min进行搅拌；将多聚磷酸钠溶液加入壳聚糖溶液中,待形成沉淀后离心,去除沉淀,去离子水清洗,超声处理后,再次离心,取沉淀,风干,得壳聚糖纳米颗粒乳牙根管充填材料。使用方便,可降解,安全性高,可提高手术疗效。(A chitosan nanoparticle deciduous tooth root canal filling material and a preparation method thereof belong to the field of medical materials. The chitosan nanoparticle deciduous tooth root canal filling material comprises 99-50% of chitosan and 1-50% of a loaded drug by mass percent, wherein the chitosan is a main body bracket material, and the loaded drug is an adjusting material for adjusting the degradation rate of the material and increasing the treatment effect. Adding chitosan into a glacial acetic acid solution, stirring for dissolving, adjusting the pH value by using an alkaline liquid, placing the chitosan solution on a magnetic stirrer, and stirring at 750-1000 r/min; adding a sodium polyphosphate solution into a chitosan solution, centrifuging after a precipitate is formed, removing the precipitate, washing with deionized water, performing ultrasonic treatment, centrifuging again, taking the precipitate, and drying in the air to obtain the chitosan nanoparticle deciduous tooth root canal filling material. Convenient use, degradability and high safety, and can improve the curative effect of the operation.)

1. The chitosan nanoparticle deciduous tooth root canal filling material is characterized by comprising 99-50% of chitosan and 1-50% of a loaded drug by mass percent, wherein the chitosan is a main body bracket material, and the loaded drug is an adjusting material for adjusting the degradation rate of the material and increasing the treatment effect.

2. The root canal filling material of claim 1, wherein the chitosan nanoparticle-containing deciduous teeth is formed by uniformly coating the chitosan nanoparticle with the drug to be loaded or mixing the chitosan nanoparticle-containing deciduous teeth with the drug to be loaded, and the chitosan nanoparticle-containing deciduous teeth and the drug to be loaded are independently present.

3. The chitosan nanoparticle deciduous tooth root canal filling material according to claim 1, wherein the carried drug is a powdered drug, a water-soluble drug, a fat-soluble drug, an acid-base liquid-soluble drug.

4. The chitosan nanoparticle deciduous tooth root canal filling material according to claim 1, wherein the carried drug is selected from ZnO, growth factors or other antibacterial and anti-inflammatory drugs.

5. The chitosan nanoparticle deciduous tooth root canal filling material according to claim 4, wherein the growth factor is selected from one of fibroblast growth factor, connective tissue growth factor, platelet-derived growth factor- β; the other antibacterial and anti-inflammatory medicines are nano-scale granular medicines, the nano-scale granular medicines are selected from one of nano-silver, soluble medicines, Chinese herbal medicines and Chinese patent medicines, and the Chinese patent medicines are selected from one of tetracycline, amoxicillin, nitrofiling, emodin, puerarin, hedysarum polybotrys polysaccharide and lactoferritin; the soluble medicine is selected from vitamin C and lidocaine hydrochloride which has the functions of promoting tissue healing, inhibiting bacteria and relieving pain; the Chinese herbal medicine is selected from epimedium and a medicine with the functions of promoting blood circulation, inhibiting bacteria and promoting tissue growth.

6. The root canal filling material of claim 1, wherein the chitosan nanoparticle is selected from the group consisting of ionic gel method, covalent cross-linking method, desolvation method, emulsion droplet agglomeration method, emulsion diffusion method, macromolecular complex method, and self-assembly method.

7. The chitosan nanoparticle deciduous tooth root canal filling material according to claim 1, wherein the loaded drug is mixed with the chitosan nanoparticle by mixing the drug with a chitosan solution to form the nanoparticle, or by directly physically mixing the drug with the chitosan nanoparticle, or by drying a chitosan-soaked drug solution; the mass fraction of the chitosan solution can be 2-40%.

8. The method for preparing the chitosan nanoparticle deciduous tooth root canal filling material according to claim 1, comprising the steps of:

1) adding chitosan into a glacial acetic acid solution, stirring for dissolving, adjusting the pH value by using an alkaline liquid, placing the chitosan solution on a magnetic stirrer, and stirring at 750-1000 r/min;

2) adding a sodium polyphosphate solution into a chitosan solution, centrifuging after a precipitate is formed, removing the precipitate, washing with deionized water, performing ultrasonic treatment, centrifuging again, taking the precipitate, and drying in the air to obtain the chitosan nanoparticle deciduous tooth root canal filling material.

9. The method for preparing the chitosan nanoparticle deciduous tooth root canal filling material according to claim 8, wherein in the step 1), the ratio of the glacial acetic acid solution to the chitosan is (15-30 mL): 15-30 mg, wherein the glacial acetic acid solution is calculated by volume and the chitosan is calculated by mass; the percentage concentration of the glacial acetic acid solution is 0.7-1.5%; the alkaline liquid can be selected from sodium hydroxide or potassium hydroxide and the like, and the pH value can be 4.6-6.0.

10. The method for preparing the chitosan nanoparticle deciduous tooth root canal filling material according to claim 8, wherein in the step 2), the addition amount of the sodium polyphosphate solution is 3 to 8mL, and the concentration of the sodium polyphosphate solution is 0.7 to 2 mg/mL; centrifuging at 12000-20000 r/min after the precipitate is formed; the deionized water cleaning can be performed for 2-5 times.

Technical Field

The invention belongs to the field of medical materials, and particularly relates to an absorbable drug-carrying chitosan nanoparticle deciduous tooth root canal filling material and a preparation method thereof.

Background

Since the first application of PGA (polyglycolic acid) to clinical orthopedics in Rokkane in 1984, experiments and clinical researches on biodegradable absorbable materials have been rapidly developed, and certain results have been achieved. Such materials are currently rapidly developing in trauma orthopedics.

Deciduous teeth, which are a special body component, are gradually absorbed by the tooth root with age and eventually fall off, so that the root canal filling material of the deciduous teeth must be absorbable. The deciduous tooth root tip is absorbed, and the material in the root canal is in direct contact with the bone tissue, so the material must be a biosafety material. The root canal treatment of deciduous teeth is difficult to fill tightly, and therefore, it is preferable that the filled drug sustainably release a bacteriostatic component or a drug containing a curable component. The deciduous tooth root canal therapy is difficult to fill tightly, and the nano particles can well simulate the human tissue structure, thereby showing obvious advantages. Combining the above characteristics, PLGA nanoparticles are a suitable choice.

Disclosure of Invention

The invention aims to provide an absorbable drug-loaded chitosan nanoparticle deciduous tooth root canal filling material and a preparation method thereof.

The chitosan nanoparticle deciduous tooth root canal filling material comprises 99-50% of chitosan and 1-50% of a loaded drug by mass percent, wherein the chitosan is a main body bracket material, and the loaded drug is an adjusting material for adjusting the degradation rate of the material and increasing the treatment effect.

The chitosan and the carried medicine form a structure that the medicine is uniformly wrapped in chitosan nano particles or mixed with the chitosan, and the chitosan and the carried medicine exist independently.

The carried medicine can be powdered medicine, water-soluble medicine, fat-soluble medicine, acid-base liquid dissolved medicine and the like; the carried medicine can be selected from ZnO, growth factor or other antibacterial and anti-inflammatory medicines, and the growth factor can be one of fibroblast growth factor, connective tissue growth factor, platelet-derived growth factor-beta, and the like; the other antibacterial and anti-inflammatory medicines can be nano-scale granular medicines, the nano-scale granular medicines can be selected from one of nano-silver, soluble medicines, Chinese herbal medicines, Chinese patent medicines and the like, and the Chinese patent medicines can be selected from one of tetracycline, amoxicillin, metronidazole, emodin, puerarin, hedysari polysaccharide, lactoferritin and the like; the soluble medicine can be selected from vitamin C, lidocaine hydrochloride and other medicines with effects of promoting tissue healing, inhibiting bacteria and relieving pain; the Chinese herbal medicine can be selected from herba Epimedii, radix Zanthoxyli, etc. with effects of promoting blood circulation, inhibiting bacteria and promoting tissue growth.

The chitosan nanoparticle deciduous tooth root canal filling material can adopt one of, but not limited to, an ionic gel method, a covalent cross-linking method, a solvent removing method, an emulsion droplet aggregation method, an emulsion diffusion method, a macromolecule composite method, a self-assembly method and the like.

The method for mixing the loaded drug and the chitosan nanoparticles can be that the drug is mixed with chitosan solution to form nanoparticles, or the drug and the chitosan nanoparticles are directly physically mixed, or chitosan is soaked in the drug solution and dried to form nanoparticles; the mass fraction of the chitosan solution can be 2-40%.

The preparation method of the chitosan nanoparticle deciduous tooth root canal filling material comprises the following steps:

1) adding chitosan into a glacial acetic acid solution, stirring for dissolving, adjusting the pH value by using an alkaline liquid, placing the chitosan solution on a magnetic stirrer, and stirring at 750-1000 r/min;

in the step 1), the ratio of the glacial acetic acid solution to the chitosan can be (15-30 mL): 15-30 mg), wherein the glacial acetic acid solution is calculated by volume, and the chitosan is calculated by mass; the percentage concentration of the glacial acetic acid solution can be 0.7-1.5%; the alkaline liquid can be selected from sodium hydroxide or potassium hydroxide and the like, and the pH value can be 4.6-6.0.

2) Adding a sodium polyphosphate solution into a chitosan solution, centrifuging after a precipitate is formed, removing the precipitate, washing with deionized water, performing ultrasonic treatment, centrifuging again, taking the precipitate, and drying in the air to obtain the chitosan nanoparticle deciduous tooth root canal filling material.

In the step 2), the addition amount of the sodium polyphosphate solution can be 3-8 mL, and the concentration of the sodium polyphosphate solution can be 0.7-2 mg/mL; centrifuging at 12000-20000 r/min after the precipitate is formed; the deionized water cleaning can be performed for 2-5 times.

The invention is provided on the basis of fully considering the characteristics of pathogenic mechanism, treatment, prognosis and the like of deciduous tooth root canal filling, and the structure and the components of the prepared chitosan nanoparticle deciduous tooth root canal filling material are beneficial to the recovery and the functional recovery of dental pulp apicitis and the reduction of the recurrence rate.

The invention has the following outstanding advantages:

1) the prepared chitosan nanoparticle deciduous tooth root canal filling material can be absorbed and can not be remained in a body along with the absorption of deciduous tooth roots.

2) The prepared chitosan nanoparticle deciduous tooth root canal filling material is nanoparticles and can be well filled in deciduous tooth root canals.

3) The prepared chitosan nanoparticle deciduous tooth root canal filling material has good biocompatibility, is in contact with bone tissues in the apical area, and does not produce side effects.

4) The prepared chitosan nanoparticle deciduous tooth root canal filling material is a particle-bound medicine, can be an antibacterial medicine or a therapeutic medicine, and can promote the recovery of inflammation.

5) The prepared chitosan nanoparticle deciduous tooth root canal filling material is a novel medical drug-carrying biodegradable material, is convenient to use, degradable and high in safety, and can improve the surgical curative effect.

Detailed Description

The following examples further illustrate the invention.