阅读说明：本技术 一种葛仙米育种装置 (Nostoc sphaeroids kutz breeding device ) 是由 刘晓宏 于 2021-05-12 设计创作，主要内容包括：本发明涉及一种葛仙米育种装置,包括温控箱、诱导箱、培养箱、纯化水输送系统、培养液输送系统、光照系统、视觉系统、超声系统、通气系统、控制系统、废液系统、滤液离心系统。本发明能够自动进行葛仙米的藻殖段诱导和培育,自动化程度高、效率高、不易出错、劳动强度低,适合葛仙米规模化生产育种。(The invention relates to a nostoc sphaeroides breeding device which comprises a temperature control box, an induction box, an incubator, a purified water conveying system, a culture solution conveying system, a lighting system, a vision system, an ultrasonic system, a ventilation system, a control system, a waste liquid system and a filtrate centrifugal system. The induction and cultivation method can automatically induce and cultivate the algae cultivation section of nostoc sphaeroides, has high automation degree, high efficiency, low labor intensity and is not easy to make mistakes, and is suitable for mass production and breeding of nostoc sphaeroides.)

1. The utility model provides a nostoc sphaeroides breeding device which characterized in that: comprises a temperature control box, an induction box, an incubator, an illumination system, an ultrasonic system, a ventilation system and a control system;

the temperature control box comprises a first temperature control box and a second temperature control box;

the induction box comprises an induction box body, a slurry discharge pipe and a slurry discharge valve, the induction box body is placed in the first temperature control box, the top of the induction box body is provided with an opening, and the slurry discharge valve and the slurry discharge pipe are arranged at the bottom of the induction box body;

the incubator comprises an incubator body, a culture solution discharge pipe and a culture solution discharge valve, the incubator body is placed in the second temperature control box, an opening is formed in the top of the incubator body for the entry of a slurry, and the culture solution discharge pipe and the culture solution discharge valve are arranged at the bottom of the incubator body;

the illumination system comprises a first illumination device and a second illumination device, the first illumination device is arranged inside the induction box body and/or the first temperature control box, and the second illumination device is arranged inside the culture box body and/or the second temperature control box;

the ultrasonic system is arranged inside the induction box body and is used for promoting the cracking of the nostoc sphaeroides;

the aeration system comprises an air compressor and an air outlet pipe, one end of the air outlet pipe is connected with the air compressor, the other end of the air outlet pipe is inserted into the culture solution of the culture box body, and a plurality of air holes are formed in the pipe wall of the pipe section inserted into the culture box body;

the control system is used for controlling each system.

2. The nostoc sphaeroides breeding device according to claim 1, wherein: still include purified water conveying system, it still includes purified water feed liquor pipe to induce the case, purified water feed liquor pipe is established the top or the lateral part of induced box, purified water conveying system includes purification water tank, purified water delivery pump, purified water conveyer pipe, purified water ooff valve, the purification water tank is used for depositing purified water, the one end of purification water delivery pump with the purification water tank links to each other, the other end with the purified water conveyer pipe links to each other, the one end of purified water ooff valve with the purified water conveyer pipe links to each other, the other end with the purified water feed liquor pipe links to each other.

3. The nostoc sphaeroides breeding device according to claim 1, wherein: still include culture solution conveying system, the incubator still includes culture solution feed liquor pipe, culture solution feed liquor pipe is established the top or the lateral part of incubator body, culture solution conveying system includes culture solution box, culture solution delivery pump, culture solution conveyer pipe, culture solution switch valve, the culture solution box is used for depositing the culture solution, the one end of culture solution delivery pump with the culture solution box links to each other, and the other end with the culture solution conveyer pipe links to each other, the one end of culture solution switch valve with the culture solution conveyer pipe links to each other, the other end with culture solution feed liquor pipe links to each other.

4. The nostoc sphaeroides breeding device according to claim 1, wherein: still include the waste liquid system, the waste liquid system is placed the below of culture solution discharge valve for deposit discarded culture solution.

5. The nostoc sphaeroides breeding device according to claim 1, wherein: still include filtrating centrifugal system, filtrating centrifugal system includes nostoc sphaeroids kutz box, rotary mechanism, the lateral part and/or the bottom of nostoc sphaeroids kutz box are equipped with the liquid hole, rotary mechanism's one end is installed the bottom of induction box, the other end with the nostoc sphaeroids kutz box links to each other, rotary mechanism is arbitrary actuating mechanism who produces rotary motion.

6. The nostoc sphaeroides breeding device according to claim 1, wherein: the visual system is arranged inside the induction box body and/or the first temperature control box and is used for observing the color of the nostoc sphaeroides.

7. The nostoc sphaeroides breeding device according to claim 1, wherein: the induction box body is made of a light-transmitting material.

8. The nostoc sphaeroides breeding device according to claim 1, wherein: the frequency of the ultrasonic system is 20 KHz-100 KHz.

9. The nostoc sphaeroides breeding device according to claim 1, wherein: the temperature of the first temperature control box is 35-40 ℃, and the temperature of the second temperature control box is 15-33 ℃.

10. The nostoc sphaeroides breeding device according to claim 1, wherein: the illumination intensity of the first illumination device is 4000-12000 lux, and the illumination intensity of the second illumination device is 500-10000 lux.

Technical Field

The invention belongs to the technical field of agricultural biology, and relates to a nostoc sphaeroides breeding device.

Background

Nostoc sphaeroides is a nitrogen-fixing blue algae in fresh water, and is one of typical nostoc. The algae breeding section propagation is a main propagation mode of nostoc, a fast and rapid algae seed breeding technology is a core technology of nostoc scale culture, and the core of the algae seed breeding technology lies in possessing an algae breeding section induction method with high speed, high activity and large quantity. In the related nostoc breeding technology, the nostoc microsphere with filamentous distribution of internal algae filaments is bred by respectively adopting an algae breeding section and homogenized algae filaments as starting points, compared with the homogenized algae filaments, the nostoc microsphere bred by the algae breeding section has more superiority, the algae breeding section has fast growth and short period, and the nostoc sphere formed by the algae breeding section has higher capacity of coping with environmental mutation. The algae breeding section is a short-chain algae filament with 'pseudo vacuole' and is released by the nostoc sphaeroids body through spontaneous rupture under the condition that the nostoc sphaeroids body is not suitable for the change of the external environment, when the environment is changed violently, the large-particle size nostoc sphaeroids is weaker in the response capability to the environmental mutation, the initial expression is that the color of the nostoc sphaeroids is changed from black to grey green, then the surfaces of nostoc sphaeroids groups begin to be dissolved, the nostoc sphaeroids loses the penetration protection effect of a gum sheath, the algae breeding section is released gradually, but in a natural state, the nostoc sphaeroids releases the algae breeding section at a low frequency to breed, the number of the released algae breeding section is extremely small, and the requirement of mass production and breeding of the nostoc sphaeroids is difficult to meet.

Therefore, it is urgently needed to develop a device for inducing the nostoc sphaeroides algae breeding section to meet the requirement of mass production and breeding of nostoc sphaeroides.

Disclosure of Invention

Aiming at the problems, the invention provides a nostoc sphaeroides breeding device which can automatically induce the algae breeding section of nostoc sphaeroides, has high automation degree and is suitable for mass production and breeding of nostoc sphaeroides.

The technical scheme adopted by the invention for solving the technical problems is as follows: a nostoc sphaeroides breeding device comprises a temperature control box, an induction box, an incubator, an illumination system, an ultrasonic system, a ventilation system and a control system.

Preferably, the temperature control box comprises a first temperature control box and a second temperature control box, the temperature of the first temperature control box is 35-40 ℃, and the temperature of the second temperature control box is 15-33 ℃.

Preferably, the induction tank includes an induction tank body placed in the first temperature control tank, a slurry discharge pipe having an open top, and a slurry discharge valve provided at a bottom of the induction tank body.

Preferably, the induction box further comprises a purified water inlet pipe, and the purified water inlet pipe is arranged at the top or the side of the induction box body.

Preferably, the induction box body is made of a light-transmitting material.

Preferably, the material of the induction box body is one of transparent acrylic plates, glass, resin, polycarbonate, organic glass and polystyrene.

Preferably, the incubator comprises an incubator body, an incubation liquid discharge pipe and an incubation liquid discharge valve, the incubator body is placed in the second temperature control box, an opening is formed in the top of the incubator body for allowing the entry of the plasma, and the incubation liquid discharge pipe and the incubation liquid discharge valve are arranged at the bottom of the incubator body.

Preferably, the incubator further comprises a culture solution inlet pipe, and the culture solution inlet pipe is arranged at the top or the side of the incubator body.

Preferably, the culture solution comprises the following components: adding MgSO4 & 7H 2O: 75 mg/L; k2HPO4 & 3H 2O: 40 mg/L; EDTA-2 Na: 1 mg/L; CaCl2 & 2H 2O: 9 mg/L; citric acid: 6 mg/L; ferric ammonium citrate: 6 mg/L; na2CO 3: 20 mg/L; h3 BO 3: 2.86 g/L; MnCl2.4H2O: 1.81 g/L; ZnSO4 & 7H 2O: 222 mg/L; na2MoO4 · 2H 2O: 39 mg/L; CuSO4 & 5H 2O: 79 mg/L; co (NO3) 2.6H 2O, 49 mg/L.

Preferably, the illumination system comprises a first illumination device and a second illumination device, the first illumination device is installed inside the induction box body and/or the first temperature control box, and the second illumination device is installed inside the culture box body and/or the second temperature control box.

Preferably, the first illumination device has an illumination intensity of 4000 to 12000 lux.

Preferably, the illumination intensity of the second illumination device is 500-10000 lux.

Preferably, the ultrasonic system is installed inside the induction box body, is used for promoting the cracking of the nostoc sphaeroides, and has adjustable ultrasonic frequency, and the technology adopted by the ultrasonic system is the prior art and is not detailed here.

Preferably, the frequency of the ultrasonic system is 20 KHz-100 KHz.

Preferably, the ventilation system comprises an air compressor and an air outlet pipe, one end of the air outlet pipe is connected with the air compressor, the other end of the air outlet pipe is inserted into the culture solution of the culture box body, and a plurality of air holes are formed in the pipe wall of the pipe section inserted into the culture box body.

Preferably, the control system is used for controlling each system.

Preferably, the induction box further comprises a vision system, wherein the vision system is arranged inside the induction box body and/or the first temperature control box and is used for observing the color of the nostoc sphaeroides.

Preferably, still include purified water conveying system, purified water conveying system includes purification water tank, purified water delivery pump, purified water conveyer pipe, purified water ooff valve, the purification water tank is used for depositing purified water, the one end of purified water delivery pump with the purification water tank links to each other, the other end with the purified water conveyer pipe links to each other, the one end of purified water ooff valve with the purified water conveyer pipe links to each other, the other end with the purified water feed liquor pipe links to each other.

Preferably, the culture medium conveying system comprises a culture medium box, a culture medium conveying pump, a culture medium conveying pipe and a culture medium switch valve, wherein the culture medium box is used for storing culture medium, one end of the culture medium conveying pump is connected with the culture medium box, the other end of the culture medium conveying pump is connected with the culture medium conveying pipe, one end of the culture medium switch valve is connected with the culture medium conveying pipe, and the other end of the culture medium switch valve is connected with a culture medium liquid inlet pipe.

Preferably, a waste liquid system is further included, the waste liquid system being disposed below the culture liquid discharge valve for storing the discarded culture liquid.

Preferably, the liquid filter further comprises a filtrate centrifugal system, the filtrate centrifugal system comprises a nostoc sphaeroides box body and a rotating mechanism, liquid passing holes are formed in the side part and/or the bottom of the nostoc sphaeroides box body, one end of the rotating mechanism is installed at the bottom of the induction box body, and the other end of the rotating mechanism is connected with the nostoc sphaeroides box body.

Preferably, the rotating mechanism is any driving mechanism generating a rotating motion.

Preferably, the rotating mechanism is one of an electric motor, a hydraulic motor and a pneumatic motor.

Compared with the prior art, the invention has the following beneficial effects:

1. the algae breeding section induction of the nostoc sphaeroides can be automatically carried out;

2. the control system is used for accurately controlling the breeding process, so that the influence of manual operation on breeding is reduced;

3. is suitable for automation, has low requirement on operators, reduces the labor intensity of the operators and is suitable for mass production and breeding of the nostoc sphaeroides.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic structural view of the induction box 12 of the present invention;

FIG. 3 is a schematic view of the structure of the incubator 13 of the present invention;

FIG. 4 is a schematic diagram of the purified water delivery system 14 of the present invention;

FIG. 5 is a schematic view showing the structure of a culture solution feeding system 15 of the present invention.

Detailed Description

The present invention will be described in detail with reference to fig. 1 to 5, wherein the exemplary embodiments and descriptions of the present invention are provided to explain the present invention, but not to limit the present invention.

An induced breeding device for a nostoc sphaeroides breeding section comprises a temperature control box 11, an induction box 12, an incubator 13, a purified water conveying system 14, a culture solution conveying system 15, an illumination system 16, a visual system 17, an ultrasonic system 18, a ventilation system 19, a control system 20, a waste liquid system 21 and a filtrate centrifugal system 22.

The temperature control box 11 includes a first temperature control box 111 and a second temperature control box 112, wherein the temperature of the first temperature control box 111 is set to 35-40 ℃, and the temperature of the second temperature control box 112 is set to 15-33 ℃.

The induction box 12 includes an induction box body 121, a slurry discharge pipe 122, a purified water inlet pipe 123, and a slurry discharge valve 124, the induction box body 121 is placed in the first temperature control box 111, the top of the induction box body 121 is open, the purified water inlet pipe 123 is provided at the top or side of the induction box body 121, and the slurry discharge valve 124 and the slurry discharge pipe 122 are provided at the bottom of the induction box body 121.

The incubator 13 includes an incubator body 131, a culture solution inlet pipe 132, a culture solution outlet pipe 133, and a culture solution outlet valve 134, wherein the incubator body 131 is disposed in the second temperature control box 112, an opening is formed at the top of the incubator body for the entry of the culture solution, the culture solution inlet pipe 132 is disposed at the top or side of the incubator body 131, and the culture solution outlet pipe 133 and the culture solution outlet valve 134 are disposed at the bottom of the incubator 131.

The purified water delivery system 14 includes a purified water tank 141, a purified water delivery pump 142, a purified water delivery pipe 143, and a purified water switch valve 144, wherein the purified water tank 141 is used for storing purified water, one end of the purified water delivery pump 142 is connected to the purified water tank 141, the other end thereof is connected to the purified water delivery pipe 143, one end of the purified water switch valve 144 is connected to the purified water delivery pipe 143, and the other end thereof is connected to the purified water inlet pipe 123.

The culture solution delivery system 15 includes a culture solution tank 151, a culture solution delivery pump 152, a culture solution delivery pipe 153, and a culture solution switch valve 154, wherein the culture solution tank 151 is used for storing culture solution, one end of the culture solution delivery pump 152 is connected with the culture solution tank 151, the other end is connected with the culture solution delivery pipe 153, one end of the culture solution switch valve 154 is connected with the culture solution delivery pipe 153, and the other end is connected with the culture solution inlet pipe 132.

The illumination system 16 comprises a first illumination device 161 and a second illumination device 162, wherein the first illumination device 161 is installed inside the induction box body 121 and/or the first temperature control box 111, the illumination intensity is 4000-12000 lux, and the second illumination device 162 is installed inside the culture box body 131 and/or the second temperature control box 112, the illumination intensity is 500-10000 lux.

The vision system 17 is installed inside the induction box body 121 and/or the first temperature control box 111 for observing the color of nostoc sphaeroides.

The ultrasonic system 18 is installed inside the induction box body 121, the ultrasonic frequency is adjustable, and the frequency of the ultrasonic system is 20 KHz-100 KHz.

The aeration system 19 comprises an air compressor 191 and an air outlet pipe 192, one end of the air outlet pipe 192 is connected with the air compressor 191, the other end of the air outlet pipe 192 is inserted into the culture solution of the culture box body 131, and the air outlet pipe 192 is provided with a plurality of air holes on the pipe wall of the pipe section inserted into the culture box body 131.

The control system 20 is used for controlling the respective systems.

The waste liquid system 21 is disposed below the culture liquid discharge valve 134 for storing the discarded culture liquid.

The filtrate centrifugal system 22 includes a nostoc sphaeroides box 221 and a rotating mechanism 222, wherein the side and/or bottom of the nostoc sphaeroides box 221 is provided with a liquid passing hole, one end of the rotating mechanism 222 is installed at the bottom of the inducing box 121, the other end is connected with the nostoc sphaeroides box 221, and the rotating mechanism 222 is a motor.

The culture solution comprises the following components: adding MgSO4 & 7H 2O: 75 mg/L; k2HPO4 & 3H 2O: 40 mg/L; EDTA-2 Na: 1 mg/L; CaCl2 & 2H 2O: 9 mg/L; citric acid: 6 mg/L; ferric ammonium citrate: 6 mg/L; na2CO 3: 20 mg/L; h3 BO 3: 2.86 g/L; MnCl2.4H2O: 1.81 g/L; ZnSO4 & 7H 2O: 222 mg/L; na2MoO4 · 2H 2O: 39 mg/L; CuSO4 & 5H 2O: 79 mg/L; co (NO3) 2.6H 2O, 49 mg/L.

The specific implementation process comprises the following implementation steps:

s1, starting and initializing the system;

s2, putting the selected nostoc sphaeroides with mellow shape and large particle size into a nostoc sphaeroides box body 221;

s3, opening the purified water switch valve 144, starting the purified water delivery pump 142, delivering the purified water from the purified water tank 141 to the induction tank 121, entering the nostoc sphaeroides tank 221 through the liquid inlet hole of the nostoc sphaeroides tank 221, and stopping the purified water delivery pump 142 after the set flow rate is reached;

s4, starting the first illumination device 161, wherein the illumination intensity is 4000-12000 lux;

s5, standing and culturing for a set time or judging that the nostoc sphaeroides is gray green according to the vision system 17 to obtain a gray green nostoc sphaeroides group with a release signal of a algae breeding section, starting the ultrasonic system 18, and closing the ultrasonic system 18 after the set time is reached to break the nostoc sphaeroides;

s6, starting the rotating mechanism 222 to drive the nostoc sphaeroides box body 221 to rotate, so that filtrate containing nostoc sphaeroides is discharged into the induction box body 121 through the liquid inlet hole;

s7, opening the slurry discharge valve 124 to discharge the filtrate containing nostoc sphaeroides section to the culture box 131;

s8, opening the culture solution switch valve 154, starting the culture solution delivery pump 152, delivering the culture solution from the culture solution box 151 to the culture box body 131, and stopping the culture solution delivery pump 152 after the set flow rate;

s9, starting the second illumination device 162, wherein the illumination intensity is 500-10000 lux;

s10, starting the air compressor 191, and allowing air to enter the culture solution through the air outlet pipe 192 to culture the algae section to obtain nostoc sphaeroides in a microsphere form.

The technical solutions provided by the embodiments of the present invention are described in detail above, and the principles and embodiments of the present invention are explained herein by using specific examples, and the descriptions of the embodiments are only used to help understanding the principles of the embodiments of the present invention; meanwhile, for a person skilled in the art, according to the embodiments of the present invention, there may be variations in the specific implementation manners and application ranges, and in summary, the content of the present description should not be construed as a limitation to the present invention.

In the description of the present invention, it is to be understood that the terms indicating an orientation or positional relationship are based on the orientation or positional relationship shown in the drawings only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings or the orientations or positional relationships that the products of the present invention are conventionally placed in use, and are only used for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the devices or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.

Furthermore, the terms "horizontal", "vertical", "overhang" and the like do not imply that the components are required to be absolutely horizontal or overhang, but may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.

In the present invention, unless otherwise expressly stated or limited, the first feature may be present on or under the second feature in direct contact with the first and second feature, or may be present in the first and second feature not in direct contact but in contact with another feature between them. Also, the first feature being above, on or above the second feature includes the first feature being directly above and obliquely above the second feature, or merely means that the first feature is at a higher level than the second feature. A first feature that underlies, and underlies a second feature includes a first feature that is directly under and obliquely under a second feature, or simply means that the first feature is at a lesser level than the second feature.